--- Day changed Sun Mar 01 2009 00:00 < xp_prg> no, I have a refrigerator with food in it 00:00 < xp_prg> I just put the stuff in there 00:00 < kanzure> fenn: dpkg was yakking about a dependency to libvtk5-qt4 specifically. 00:01 < kanzure> xp_prg: I would strongly discourage mixing biological material storage with food storage. 00:01 < Gene_H> you need about 3 peltier coolers 00:01 < fenn> wah 00:01 < Gene_H> stack them on top of each other 00:01 < Gene_H> power up 00:01 < Gene_H> put in fridge 00:01 < kanzure> don't put in fridge 00:01 < kanzure> blargh. 00:01 * kanzure worries. 00:01 < Gene_H> gets down to very cold 00:01 < xp_prg> dude I just did 00:01 < Gene_H> -60 freezer temp 00:02 < xp_prg> so like what temp does it need to be at? 00:02 < fenn> put ice in a bucket 00:02 < Gene_H> Kanzure don't worry about it, I've done stuff like that and nothing bad ever happened to me 00:02 < fenn> or styrofoam box if you're fancy 00:02 < kanzure> xp_prg: pop quiz. is there anything wrong with storing your gel runs in a refridgerator? 00:02 < xp_prg> don't know, but I am just storing the raw contents that came in the box 00:02 < Gene_H> xp_prg description of what you are doing? 00:02 < kanzure> specifically a refrigerator that you use for food 00:03 * kanzure reads the elmergui manual 00:03 < kanzure> http://www.nic.funet.fi/index/elmer/doc/ElmerGUIManual.pdf 00:03 < katsmeow-afk> just an fyi you can discard, but if you do this on a larger scale, some dept stores now sell compact fridges for under $100 00:04 < xp_prg> Gene_H I ordered this: 00:04 < xp_prg> Items 00:04 < xp_prg> Item# ItemDescription QtyShipped 00:04 < xp_prg> 211081 TRANS, GREEN GENE 4 STN KIT 1 00:04 < xp_prg> 211081A TRANS, GREEN GENE 4 STN KIT 1 00:04 < xp_prg> 211082C TRANS, GREEN GENE4&8STN REFILL 1 00:04 < Gene_H> heh 00:04 < Gene_H> LABEL IT AS NOT FOOD 00:05 < kanzure> Gene_H: you're an idiot 00:05 < kanzure> that's not the point 00:05 < xp_prg> so like do you think the cells died and stuff? 00:05 < kanzure> fenn: wash your hands of this diybio shit while you still can 00:05 < Gene_H> well how'd they ship it? 00:05 < xp_prg> in a box 00:05 < Gene_H> with? 00:05 < Gene_H> dry ice? 00:05 < xp_prg> no 00:05 < fenn> kanzure: there are some smart people, they'll figure out how to make it work 00:05 < Gene_H> well you may not need to 00:05 < kanzure> ever wonder why biology labs mount fucking turrets next to food refrigerators and shoot anyone with anything remotely looking like biological materials? 00:06 < katsmeow-afk> yes 00:06 < Gene_H> kanzure did you hear the story about the people who developed artificial urine? 00:06 < kanzure> Gene_H: artificial urea? 00:06 < xp_prg> hello is my stuff messed up or not? 00:06 < fenn> xp_prg: go fuck yourself 00:06 < Gene_H> yeah for calibrating urine testing machines 00:06 < kanzure> yay fenn. 00:06 < xp_prg> hahhaha 00:07 < xp_prg> how come? 00:07 < kanzure> xp_prg: I don't think you understand us 00:07 -!- willPow3r_ [n=will@cpe-66-75-6-181.san.res.rr.com] has quit [Read error: 110 (Connection timed out)] 00:07 < kanzure> did I or did I not mention any health hazards in the last few minutes 00:07 < fenn> you're such an ignorant demanding twit 00:07 < xp_prg> dude is my shit fucked up or not? 00:07 < Gene_H> xp_prg, you idiot, you have to use staphlococcus 00:07 < kanzure> another pop quiz: is "your shit being fucked up" the biggest of your worries? 00:07 < xp_prg> dude I just paid 80 bux for this thing 00:07 < kanzure> what does that have to do with what I just asked you 00:08 < xp_prg> its in containers and stuff I am sure it is safe while in the containers right? 00:08 < xp_prg> I didn't open the little containers or anything 00:08 < Gene_H> so what are you going to do next? 00:08 < kanzure> Gene_H: please stop for the moment 00:08 < kanzure> this is kind of serious. 00:08 < xp_prg> well I am going to take it sf next weekend 00:10 < Gene_H> ok, it seems you have no idea as to what you are doing 00:12 < Gene_H> so the best way to preserve biological samples is to add a cryoprotectant 00:13 < xp_prg> ok do you think my cells died and stuff though? 00:13 < Gene_H> a cryoprotectant allows biological samples to be stored at higher temperatures 00:13 < Gene_H> common household bleach is a great cryoprotectant 00:13 < Gene_H> if used in small quantities 00:13 < fenn> huh. dpkg -i foo aborted and then apt-get -f install will get all the required packages for foo 00:14 < kanzure> fenn: yes, that happened to me too 00:14 < kanzure> I found it strange that it knew to do that 00:14 < Gene_H> if you use it in high quantities your cells die 00:14 < Gene_H> dilute it to about 20% 00:14 < kanzure> Gene_H: quantities? volumes maybe? 00:14 < Gene_H> yes volumes 00:16 < Gene_H> less than a drop should do 00:21 < Gene_H> remember, IT HAS TO BE LESS THAN A DROP or it doesn't work 00:22 < kanzure> http://web.mit.edu/elmer_v5.3/tutorials_files/ hm. Maybe I'll use some of these. 00:23 < Gene_H> firefox crashed again 00:23 < kanzure> too many tabs. 00:23 < fenn> it seems elmerf90 wants to compile a file every time? 00:23 < kanzure> every time what? 00:23 < fenn> for each simulation 00:23 < xp_prg> you guys are so hard to get help from 00:24 < fenn> we are not DIYbio tech support 00:24 < fenn> try #diybio (gasp) 00:24 < fenn> or better yet, read a book 00:25 < Gene_H> xp_prg, you have to add a cryoprotectant for you to store it in your refrigerator 00:26 < fenn> kanzure: the elmerfem-doc package has some nice pdf's 00:26 < xp_prg> Gene_H does it need to be refrigerated or not? 00:26 < xp_prg> it says store in a dry cool place 00:26 < kanzure> fenn: where are they put? /usr/share/blah/ ? 00:26 < fenn> /usr/share/doc/elmerfem-doc/ 00:27 < fenn> btw dpkg -S is your minion 00:27 < kanzure> aha. 00:27 < fenn> i should mention apt-file while i'm at it 00:28 < Gene_H> IT NEEDS TO BE REFRIGERATED, BUT YOU HAVE TO ADD A CRYOPROTECTANT 00:28 < fenn> oh FFS the reason i was having a hard time is it's ElmerGUI not elmergui 00:28 < Gene_H> if you don't add a cryoprotectant it just dies 00:29 < xp_prg> dude I don't hae a cryoprotectant shit 00:30 < fenn> cells will survive at room temp but they won't stay competent 00:30 * xp_prg kills himself 00:30 < xp_prg> well I just put it in the fridge I hope that helps 00:30 < kanzure> that's what we've been getting at. 00:32 < fenn> aw man it's one of those apps that segfaults if you look at it wrong 00:32 < kanzure> yeah :( 00:32 < kanzure> but apparently there are some tools under the hood that should be used anyway 00:32 < kanzure> if I can figure out how. looking at those PDFs now. 00:32 < kanzure> or first the examples. 00:35 < Gene_H> WHAT'S THIS! NOW ADOBE ACROBAT IS CRASHING ON ME! 00:36 < kanzure> ooh, ElmerTutorials.pdf *is* nice. 00:37 < Gene_H> and I can't read it, because ADOBE is broken 00:37 < kanzure> you don't even have dpkg 00:53 < fenn> it seems like it runs but i dont get any colors in the result (just all blue) 00:53 < kanzure> that's farther than I've gotten 00:53 < kanzure> how'd you manage to get there? 00:56 < kanzure> neat, there's a t-cross microfluidic tutorial 00:56 < fenn> copy /usr/share/elmerfem-doc/tutorials_files to your ~ 00:56 < katsmeow-afk> wierd, i just had to kill adobe too, it was hung but using 97%cpu forever 00:56 < fenn> then open TemperatureAngleGUI/angle.grd 00:56 < fenn> then run-> run solver; then start postprocessor and postprocess and open case.ep 00:57 -!- cis-action [n=cis-acti@146-115-127-170.c3-0.smr-ubr1.sbo-smr.ma.cable.rcn.com] has quit [] 00:58 < fenn> i dunno how to run the non-GUI tutorials 00:58 < kanzure> hrm. 00:58 < kanzure> okay, I see the all-blues. 00:58 < fenn> i guess i should follow the tutorial pdf 00:59 < kanzure> fenn: the TemperatureAngleGUI demo seems to only be showing the first timestep 00:59 < kanzure> erm, nevermind 01:00 < kanzure> the output file is data3d.ep according to one of the model config menus. 01:02 < kanzure> HeatSolve: Result Norm : 7.78240309139357489E-002 01:02 < kanzure> HeatSolve: Relative Change : 2.34494291199996476E-011 01:02 < kanzure> E-011, geeze. 01:03 < xp_prg> what you guys doing? 01:03 < fenn> hm crashes every time i click on 'edit solver settings' in the equations window 01:03 < kanzure> same here. 01:04 < fenn> ok i'll just not do that 01:04 < fenn> where do i set density and heat conductivity? 01:04 < kanzure> model -> material 01:07 < kanzure> I never see anything like heat* under model->equation even though some screenshots show that there should be something 01:07 -!- Gene [i=Gene@wireless-128-62-97-56.public.utexas.edu] has joined #hplusroadmap 01:14 < kanzure> I think the "edit solver settings" crash bug is probably critical to all of this, fenn. 01:14 < kanzure> http://elmerfem.wiki.sourceforge.net/Getting+started+with+ElmerGUI might give a hint as to what "Edit Solver Settings" is for. 01:15 < fenn> they should just provide a .sif file 01:17 < kanzure> some of the sif files seem to have weird paths in ! lines at the top 01:17 < kanzure> which might be why the non-GUI tutorials do not work 01:18 < kanzure> erm, except for not having the file you actually need 01:18 < kanzure> (grd) 01:18 < kanzure> ah neat, RayleighBernardGUI now runs 01:25 -!- gene_haecker [i=Gene@wireless-128-62-97-56.public.utexas.edu] has joined #hplusroadmap 01:25 -!- Gene [i=Gene@wireless-128-62-97-56.public.utexas.edu] has quit [] 01:26 -!- gene_haecker [i=Gene@wireless-128-62-97-56.public.utexas.edu] has quit [Client Quit] 01:26 < Gene_H> ok kanzure, I' 01:27 < Gene_H> accidently a whole adobe acrobat 01:33 < fenn> ok well this has ceased being fun 01:34 < fenn> i thought from the pretty screenshots that it would .. work 01:34 < kanzure> seems to be working for me (it's at time=400) 01:34 < kanzure> (in the "convergence history" window) 01:35 < fenn> that just means it's not a steady state 01:35 < kanzure> eh. 01:35 < fenn> i dont get any results because i cant define the initial conditions because the GUI is fubared and all the example files are fubared 01:36 < kanzure> hm, I have 140 threads from [Elmerdiscussion] 01:38 < fenn> if you're serious about trying to get it to work i think you will have to compile from source 01:39 < kanzure> I wonder if there is a source package in that repo. 01:41 < Gene_H> that's it, screenshots 01:41 < Gene_H> I don't have to fix adobe, I can just take scrceenshots of what I'm reading 01:42 < fenn> you can just take a picture of the screen with a video camera and upload it to youtube 01:42 < Gene_H> that would reduce the quality 01:42 < fenn> make sure to slowly pan across the text and add dramatic music 01:42 < Gene_H> you know that's the way they used to make movies of video games 01:43 < Gene_H> adding music would circumvent copyright 01:43 < fenn> is that so 01:44 < Gene_H> you know the only way to make a video of gameboy games is to use a video camera? 01:44 < Gene_H> sad isn't it? 01:45 < kanzure> http://heybryan.org/shots/2009-03-01_elmer.png 01:45 < kanzure> bwahahah. 01:45 < kanzure> Gene_H: not true. 01:46 < Gene_H> I mean gameboy DS 01:46 < Gene_H> yeah I guess so 01:48 < kanzure> uh oh, why can't I access my server 01:48 < kanzure> why can't I access anything? 01:49 < kanzure> but anyway, fenn, it works. 01:49 < Gene_H> because your server has just become self-aware 01:50 < Gene_H> I can't access it too 01:51 < Gene_H> odd, adobe crashes after a preset time period 01:53 -!- PeerInfinity [n=someone@216.36.180.162] has quit [] 01:53 < Gene_H> almost like it's been rigged to 01:54 < Gene_H> oh pretty 01:54 < Gene_H> screen shot is awesome 01:55 < Gene_H> youtube? 01:57 < kanzure> http://www.physicsandmusic.com/contact.php aha, I have successfully googlestalked the nano song people. 01:58 < Gene_H> waht does googlestalk mean? 01:58 < Gene_H> physics and music eh? 01:59 < Gene_H> you know I so want to build a fluidic guitar amp 02:00 < Gene_H> damn machine shop 02:01 < kanzure> http://www.urbandictionary.com/define.php?term=googlestalk 02:04 -!- willPow3r [n=will@cpe-66-75-6-181.san.res.rr.com] has joined #hplusroadmap 02:08 < fenn> you could do a fluidic voice amp 02:09 < fenn> like a kazoo but with MORE POWER 02:10 < Gene_H> and more quality than a kazoo 02:10 < Gene_H> kazoo's just resonator 02:11 < Gene_H> perhaps it could be used as a clock in a fluidic computer 02:11 < fenn> as an oscillator, but you'd want a square wave for the clock 02:13 < fenn> kanzure: how long did that simulation take to run? 02:13 < kanzure> 5 or 10 minutes 02:15 -!- ybit [n=heath@unaffiliated/ybit] has quit [Read error: 110 (Connection timed out)] 02:15 < fenn> did you do anything in model->setup? 02:17 < kanzure> no 02:20 < Gene_H> huh I guess so 02:22 < kanzure> I can't seem to figure out how to change the number of generated resulting states, or whatever 02:23 < kanzure> in the sif file, there's timestep-sizes=1, timestep-intervals=blah, output intervals=blah, max iterations=blah, max outputlevel = blah, 02:23 < kanzure> and none of these can be knobbed into producing the apparently 4 different samples that VTK is displaying for some reason. 02:23 < kanzure> this is unnecessarily complicated. 02:27 < Gene_H> dang it 02:27 < Gene_H> I want to read those papers but I can't 02:27 < fenn> what papers? 02:27 < Gene_H> the one's kanzure sent me 02:41 -!- ybit [n=heath@unaffiliated/ybit] has joined #hplusroadmap 03:20 -!- ybit [n=heath@unaffiliated/ybit] has quit [Read error: 104 (Connection reset by peer)] 03:23 -!- ybit [n=heath@unaffiliated/ybit] has joined #hplusroadmap 03:24 -!- ybit [n=heath@unaffiliated/ybit] has quit [Read error: 104 (Connection reset by peer)] 03:28 < fenn> huh i guess it does work after all: http://fennetic.net/pub/irc/elmergui.png 03:29 < fenn> when you click on surface you have to pick a value from the menu instead of Null 03:30 < fenn> still can't figure out streamlines and isosurfaces 03:31 -!- ybit [n=heath@unaffiliated/ybit] has joined #hplusroadmap 03:51 < Gene_H> what's and isosurface? 04:01 < fenn> a surface of equal magnitude within a volume 04:02 < fenn> it's quite probably it doesnt work because i'm only doing 2d example files 04:09 < fenn> http://fennetic.net/cadwiki/index.php?isoline 04:13 -!- Gene_H [i=Gene@wireless-128-62-97-56.public.utexas.edu] has quit [Read error: 60 (Operation timed out)] 04:18 -!- xp_prg [n=xp_prg3@c-71-198-6-29.hsd1.ca.comcast.net] has quit ["This computer has gone to sleep"] 05:38 < fenn> ah finally, bash completion, how I have longed for thee 05:43 < fenn> ugh, too bad it's an ugly hack 05:44 < fenn> i thought it might use getopt but all the values are just hardcoded in /etc/bash_completion 06:29 < bkero> Hypothesis: Lua is a pain in the ass. Experiment: http://pastebin.osuosl.org/24371 Conclusion: Yep, Lua is a pain in the ass. 06:37 < UtopiahGHML> http://trac.openturns.org/ Open source initiative to Treat Uncertainties, Risks.N Statistics in a structured industrial approach 06:51 < fenn> could you explain what that means? 06:52 < fenn> or at least why you think we'd be interested 06:53 < fenn> i understand what uncertainty is and most of the distributions 06:54 < UtopiahGHML> resistance of material, thus can be used in simulations to see if a designed object will hold 06:54 < UtopiahGHML> thus useful for modelling 06:55 < UtopiahGHML> didn't try it myself so i wont be able to say more 06:55 < fenn> honestly i think it's more work than it's worth 06:56 < fenn> if something is close enough to failure for the shape of the uncertainty to matter, you should probably revise it anyway 06:57 < fenn> maybe siai would be interested for their end-of-the-world scenarios 06:57 < UtopiahGHML> well they use it in large projects (mil. nasa. etc) so I guess passed a certain treshold of investment it's a worthy filter 07:48 -!- elias` [n=me@unaffiliated/elias/x-342423] has joined #hplusroadmap 08:30 -!- ybit [n=heath@unaffiliated/ybit] has quit [Remote closed the connection] 09:46 -!- nsh [n=kaapo@c39.adsl.tnnet.fi] has joined #hplusroadmap 09:52 < fenn> featherless chicken http://www.nextnature.net/?p=668 10:35 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has joined #hplusroadmap 10:59 < fenn> katsmeow-afk: you know a bit about google right? why did they break searching for exact phrase even in quotes with + sign in front etc 11:00 < fenn> now they entirely discard punctuation no matter what 11:02 -!- PeerInfinity [n=someone@216.36.180.162] has joined #hplusroadmap 11:21 * nsh is frown 11:21 < nsh> they even try and correct +"singleterm" queries now 11:21 < nsh> fuck them in the ear already 11:42 < kanzure> programmers everywhere would jump on to any new search engine that truly allowed searching for special characters 11:42 < kanzure> just saying. 11:43 < kanzure> is my server up? down? 11:44 < kanzure> hm. out of swap space I think. 12:16 -!- katsmeow-afk [n=someone@75-120-6-201.dyn.centurytel.net] has quit [Nick collision from services.] 12:16 -!- any38774944 [n=someone@75-120-2-234.dyn.centurytel.net] has joined #hplusroadmap 12:23 < fenn> http://fab.cba.mit.edu/content/projects/mik/notes "machine invention kit" 12:24 < fenn> i think they've been monitoring my brainwaves sometimes 12:24 < fenn> "goo extrusion" 12:53 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has quit [Remote closed the connection] 12:56 -!- Overand_ [i=overand@crappy.domain.name] has joined #hplusroadmap 13:00 -!- Overand_ [i=overand@crappy.domain.name] has quit [Operation timed out] 13:03 -!- cis-action [n=cis-acti@146-115-127-170.c3-0.smr-ubr1.sbo-smr.ma.cable.rcn.com] has joined #hplusroadmap 13:06 -!- Overand [i=overand@crappy.domain.name] has quit [Read error: 113 (No route to host)] 13:25 < kanzure> " 13:25 < kanzure> insane machining > 100k RPM 13:25 < kanzure> " 13:25 < kanzure> fenn: did you get any where with elmer? 13:28 < fenn> yes but just the *GUI demos 13:28 < fenn> the crappy example files turned me off 13:29 < fenn> i have more important code to be messing with right now 13:29 < fenn> http://fennetic.net/pub/irc/elmergui.png 13:29 -!- jookos2 [n=kaapo@c39.adsl.tnnet.fi] has joined #hplusroadmap 13:29 < fenn> hi jookos2 are you associated with elmerfem? 13:30 < fenn> oh that's just nsh 13:33 < kanzure> well at least you got isosurfaces working. 13:33 < kanzure> or whatever is producing the rainbow coloration 13:33 < kanzure> maybe I'll try recompiling elmerfem. 13:40 -!- nsh [n=kaapo@c39.adsl.tnnet.fi] has quit [Read error: 113 (No route to host)] 13:41 < kanzure> http://en.wikipedia.org/wiki/Hyperthymesia another way of describing the classic 'R' case from Russia (or whatever) of the synesthesia case with amazing memory 13:42 < kanzure> oh, 'S' case - Shereshevskii 13:49 < kanzure> http://mail.mems-exchange.org/mailman/listinfo/mems-talk 13:49 < kanzure> oh shit. I've been missing out. 13:50 -!- jookos2 [n=kaapo@c39.adsl.tnnet.fi] has quit ["Your mom is so ugly, when she went to McDonalds, they couldn't serve happy meals."] 13:53 < fenn> omg a mailing list you aren't subscribed to yet! 13:56 < kanzure> found it via searching for "how to make glass hydrophobic" 13:56 < kanzure> (besides piranha solutions) 13:56 < kanzure> PMMA acrylic glass is hydrophobic apparently 13:57 -!- ybit [n=heath@unaffiliated/ybit] has joined #hplusroadmap 13:58 < fenn> i'd say spray with scotch-gard waterproofing, but you probably want something that will stick to hydrocarbon resins 13:58 < fenn> not water-repellent per-se 14:00 < kanzure> I want something that will still let sharpie be written all over it. 14:01 < kanzure> http://heybryan.org/books/Manufacturing/mems-talk_archive.zip 14:15 -!- ybit [n=heath@unaffiliated/ybit] has quit [Read error: 104 (Connection reset by peer)] 14:31 < kanzure> http://www.reefcorner.com/SpecimenSheets/bubble_algae.htm what the hell is this? 14:31 < kanzure> Valonia macroalgae 14:32 < kanzure> http://en.wikipedia.org/wiki/Valonia 14:33 -!- splicer [n=patrik@h22n2c1o261.bredband.skanova.com] has joined #hplusroadmap 14:33 < kanzure> http://images.google.com/images?client=opera&rls=en&q=valonia%20bubble%20algae&sourceid=opera&oe=utf-8&um=1&ie=UTF-8&sa=N&hl=en&tab=wi 14:34 < kanzure> http://www.aqualog.de/news/web75/75-11-13e.pdf 14:35 < kanzure> birkholtz 1986 - "killer bubbles" - a "single pod would release innumerable algal spores" 14:36 < kanzure> http://www2.hawaii.edu/~delbeek/delb5.html 14:37 < kanzure> ever seen any more. Birkholz, J. (1986): Üble Parasiten, die grünen Killerkugeln. Da 14:37 < kanzure> A more disturbing aspect of Pseudo- 14:38 < kanzure> Birkholz, J. (1986): Üble Parasiten, die grünen Killerkugeln. Das. Aquarium 20(1),31-32 14:38 * fenn gives kanzure some percussion maintenance 14:39 < kanzure> hm? 14:39 < kanzure> that tickles. stop it. 14:40 < kanzure> odd how this paper was only cited three times, all by "aqualog.de" - http://scholar.google.com/scholar?num=100&hl=en&lr=&cites=12933297891618496679 14:41 < kanzure> Valonia macrophysa - smaller clustered elongated bubbles 14:41 < kanzure> Boergesenia - clustered, though less dense in numbers and elongated bubble shapes 14:41 < kanzure> gah 14:42 < kanzure> http://www.fishlore.com/bubblealgae.htm types: valonia macrophysa, boergesenia, ventricaria ventricosa, dictosphaeria ocellata, dictosphaeria cavernosa, 14:55 < kanzure> "The voltage-dependent step of the chloride transporter of Valonia utricularis encounters a Nernst-Planck and not an Eyring type of potential energy barrier" 14:56 < kanzure> wtf, voltage-clamp studies with giant algae :D 14:57 < kanzure> oh, for sea water organisms I suppose that makes sense 15:38 -!- ybit [n=heath@unaffiliated/ybit] has joined #hplusroadmap 16:02 -!- any38774944 is now known as katsmeow-afk 17:00 < kanzure> hm. there's a whitesides paper where he shows a microfluidic device with screws acting as valves (manually controlled by humans) 17:04 -!- wrldpc [n=worldpea@173.48.207.37] has quit [] 17:31 < kanzure> why has nobody made a mf device with a polypropylene or polystyrene coupler/interconnection/"micro-to-macro" straw? 17:31 < kanzure> (and that's where gene would get to use his superglue, I guess. he really wants to use superglue.) 18:02 -!- ferrouswheel [n=jp@121-73-144-159.cable.telstraclear.net] has quit [Remote closed the connection] 18:15 -!- elias` [n=me@unaffiliated/elias/x-342423] has quit [Read error: 145 (Connection timed out)] 18:25 -!- ferrouswheel [n=jp@121-73-144-159.cable.telstraclear.net] has joined #hplusroadmap 18:27 < kanzure> Microbeads display of proteins using emulsion PCR and cell-free protein synthesis 18:27 < kanzure> say, this looks familiar.. 18:27 < kanzure> http://heybryan.org/mediawiki/index.php/Polymerase#Microbeads_display_of_proteins_using_emulsion_PCR_and_cell-free_protein_synthesis 18:27 < kanzure> (just added) 18:29 < kanzure> it didn't help that w/o meant "water/oil", not "without". 18:30 < kanzure> http://heybryan.org/books/Biology/Microbeads%20display%20of%20proteins%20using%20emulsion%20PCR%20and%20cell-free%20protein%20synthesis.pdf 18:31 -!- cis-action [n=cis-acti@146-115-127-170.c3-0.smr-ubr1.sbo-smr.ma.cable.rcn.com] has quit [] 19:00 -!- Gene [i=Gene@wireless-128-62-183-80.public.utexas.edu] has joined #hplusroadmap 19:11 < kanzure> http://heybryan.org/books/papers/microfluidics/synthesis/ 19:39 < kanzure> huh, I wonder if what I was observing yesterday with the fluid-filled cavity microchannel thingy, with the curvature of the water due to the four parallel lines, was actually a display of a cone of the water, i.e. the edges were all constrained, and water surface tension allowed there to be the formation of a cone. 19:39 < kanzure> freezing the contraption would show if that's the case methinks 19:39 < kanzure> (then just take off one of the sides and look at the ice's structure) 19:57 -!- any08937099 [n=someone@99.194.251.41] has joined #hplusroadmap 19:58 -!- katsmeow-afk [n=someone@75-120-2-234.dyn.centurytel.net] has quit [Nick collision from services.] 19:59 < kanzure> Hi any08937099. 20:05 -!- cis-action [n=cis-acti@146-115-127-170.c3-0.smr-ubr1.sbo-smr.ma.cable.rcn.com] has joined #hplusroadmap 20:12 -!- wrldpc [n=worldpea@173.48.207.37] has joined #hplusroadmap 20:17 -!- Netsplit verne.freenode.net <-> irc.freenode.net quits: cis-action, wrldpc, willPow3r, PeerInfinity, kanzure-, chizu, davidsjo, UtopiahGHML, fenn, Gene, (+8 more, use /NETSPLIT to show all of them) 20:18 -!- Netsplit over, joins: wrldpc, cis-action, any08937099, Gene, ferrouswheel, ybit, splicer, PeerInfinity, willPow3r, kanzure- (+8 more) 20:29 < kanzure> Can anyone get me the name of the contractor that did the pentagon fix-ups after 2001-09-11 ? 20:36 -!- Overand [i=overand@crappy.domain.name] has joined #hplusroadmap 20:48 -!- ybit [n=heath@unaffiliated/ybit] has quit [Read error: 104 (Connection reset by peer)] 20:53 -!- ybit [n=heath@unaffiliated/ybit] has joined #hplusroadmap 20:53 < wrldpc> prolly halliburton >:\ 21:03 < kanzure> http://tech.slashdot.org/comments.pl?sid=1144961&cid=27030617 21:06 < kanzure> RTKL 21:06 < kanzure> ftp://ftp.rtkl.com/ 21:06 < kanzure> aww. 21:14 < kanzure> http://roobishop.weebly.com/home.html <-- aww. 21:24 -!- any08937099 is now known as katsmeow1 21:40 < Gene> so what do you want with Hal kanzure? 21:52 < kanzure> does DNA sequencing by mass make sense? 21:53 < kanzure> hal? 22:11 < Gene> my dad used to work for Hal 22:11 < Gene> you know they have these trucks 22:12 < Gene> for moving mud for oil drilling operations around 22:13 < Gene> they have a band of grey on the top, so they don't have to clean them as often 22:14 < Gene> Oil pipelines also run at about 17,000 psi 22:15 < katsmeow1> 22:21 < kanzure> Gene: where does your dad work now? 22:54 < kanzure> http://heybryan.org/mediawiki/index.php/DNA_sequencer#Microfluidic_DNA_sequencing 22:54 < kanzure> (just jotting down some notes) 23:01 < kanzure> 135.13 g/mol, 126.11 g/mol, 151.13 g/mol, 111.1 g/mol (A, T, G, C). Hrm. So a mass-based sequencing approach would need to be able to discriminate at the scale of 10^-23 grams. That's not much. 23:21 -!- katsmeow1 is now known as katsmeow-afk 23:31 < kanzure> fenn: I just fwd'd you a guy who wants to pay me to make him a CNC table. 23:31 < kanzure> he emailed me, I called him back as soon as I got it, we chatted, he has a small factory in San Antonio, has some cash to spend on this 23:33 < kanzure> he already has the table, just needs the electronics installed or something 23:35 < kanzure> also, BioMors suggested a needle to poke a hole in a plastic slide for the mf-devices. I was thinking of just jamming a straw between two slides. 23:36 < Gene> whoa 23:36 < Gene> we're gonna make a cnc machine 23:36 < kanzure> Gene: what about the mechmate? 23:37 < Gene> I thought it was in no condition to work? 23:37 < Gene> what's an mf-device? 23:37 < kanzure> microfluidic 23:37 < kanzure> because microfluidic is way too much of a buzzword 23:38 < Gene> ok, so on the sharpie mfs I just found a problem 23:38 < kanzure> yeah? 23:38 < Gene> how do you pump stuff throught it 23:38 < kanzure> did you read the paper? 23:38 < Gene> ok send it to me 23:38 < Gene> never mind dont 23:38 < Gene> I got it 23:38 < kanzure> http://heybryan.org/books/papers/microfluidics/Performing%20chemical%20reactions%20in%20virtual%20capillary%20of%20surface%20tension-confined%20microfluidic%20devices%20-%20sharpies%20-%20nail%20polish%20-%20glass%20surfaces%20-%20hydrophobicity.pdf 23:39 < kanzure> basically they made one slide very hydrophobic by soaking it in piranha 23:39 < kanzure> now, if you wanted to pump it, there's a few different ways that I can imagine that happening 23:39 < kanzure> (1) seal the slides together (but make sure there's still a spacer inside) 23:39 < kanzure> (2) poke a hole with a needle, attach a straw, blow your lungs out 23:39 < kanzure> (3) possibly use electrical conduction. 23:40 < kanzure> anyway, trying it with a hydrophobic slide is a must. I just didn't do it because I didn't realize how simple a concoction piranha actually is 23:40 < kanzure> it's like hydrogen peroxide plus hydrogen sulfate 23:40 < kanzure> erm, it's H2SO4, not hydrogen sulfate. Sorry. 23:41 < kanzure> (in a 3 to 1 ratio (3 H2SO4 to 1 parts hydrogen peroxide) 23:41 < kanzure> ) 23:42 < kanzure> Principles of microfluidic actuation by modulation of surface stresses http://heybryan.org/books/papers/microfluidics/Principles%20of%20microfluidic%20actuation%20by%20modulation%20of%20surface%20stresses.pdf 23:42 < kanzure> Microchannels Constructed on Rough Hydrophobic Surfaces http://heybryan.org/books/papers/microfluidics/Microchannels%20Constructed%20on%20Rough%20Hydrophobic%20Surfaces.pdf 23:43 < kanzure> Microfluidic manipulation via Marangoni forces http://heybryan.org/books/papers/microfluidics/Microfluidic%20manipulation%20via%20Marangoni%20forces.pdf 23:43 < kanzure> An optical toolbox for total control of droplet microfluidics with lasers http://heybryan.org/books/papers/microfluidics/An%20optical%20toolbox%20for%20total%20control%20of%20droplet%20microfluidics%20with%20lasers.pdf 23:44 -!- PeerInfinity [n=someone@216.36.180.162] has quit [] 23:44 < kanzure> there was also one paper where they heat one side and thus create a temperature gradient for thermophoresis. 23:45 < kanzure> but that seems like it would just fall into equilibrium 23:45 < kanzure> and then stuff is stuck at the middle :) which is bad :) 23:55 < kanzure> Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce http://heybryan.org/books/Biology/Functional%20expression%20of%20the%20taste-modifying%20protein,%20miraculin,%20in%20transgenic%20lettuce.pdf 23:55 < kanzure> haha 23:56 < Gene> miraculin is awesome 23:57 < Gene> I've experienced it's effects 23:57 < Gene> quite interesting 23:57 < kanzure> am I going to find miraculin on erowid or something? 23:58 < Gene> hey you want to try some? 23:58 < Gene> I've got some 23:59 < Gene> In handy tablet form 23:59 < Gene> from thinkgeek