--- Day changed Tue May 26 2009 00:00 < genehacker> just a funny thing I saw online 00:00 < genehacker> convince someone to delete system32 00:47 -!- fenn_ [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has joined #hplusroadmap 01:05 -!- fenn [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has quit [Read error: 110 (Connection timed out)] 01:25 -!- fenn_ is now known as fenn 02:09 < fenn> paul really wrote 15 messages to OM today? 02:12 < genehacker> just posted my ez-DNA synth proposal to diybio 02:12 < genehacker> hopefully something useful will come of it 02:47 < fenn> budokai = martial arts society; tenkaichi = best under heaven 02:48 < fenn> literally: heaven below 1 02:49 < fenn> which would have been easy to figure out if it were written in japanese, btw 03:31 -!- elias` [n=me@resnet-pat-254.ucs.ed.ac.uk] has joined #hplusroadmap 03:46 -!- genehacker [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has quit [Read error: 104 (Connection reset by peer)] 03:46 -!- genehacker [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has joined #hplusroadmap 04:25 < faceface> genehacker: good luck! 04:29 -!- cis-action [n=cis-acti@146-115-127-170.c3-0.smr-ubr1.sbo-smr.ma.cable.rcn.com] has quit [] 06:01 -!- jm|space [n=jm@p57B9C967.dip.t-dialin.net] has joined #hplusroadmap 06:03 -!- fenn_ [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has joined #hplusroadmap 06:06 -!- jm [n=jm@p57B9F98B.dip.t-dialin.net] has quit [Read error: 60 (Operation timed out)] 06:23 -!- fenn [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has quit [Read error: 110 (Connection timed out)] 07:07 -!- cis-action [n=cis-acti@146-115-127-170.c3-0.smr-ubr1.sbo-smr.ma.cable.rcn.com] has joined #hplusroadmap 07:25 -!- Fair [n=Nofaris@adsl-75-42-83-191.dsl.scrm01.sbcglobal.net] has joined #hplusroadmap 07:54 -!- fenn [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has joined #hplusroadmap 08:12 -!- fenn_ [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has quit [Read error: 110 (Connection timed out)] 08:37 -!- Fair [n=Nofaris@adsl-75-42-83-191.dsl.scrm01.sbcglobal.net] has quit ["Leaving"] 08:48 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has joined #hplusroadmap 08:48 -!- fenn_ [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has joined #hplusroadmap 09:10 -!- fenn [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has quit [Read error: 110 (Connection timed out)] 09:53 < genehacker> downloadan opera 09:54 < genehacker> installing opera 09:54 < genehacker> does opera every experience periodic flash failure like firefox does? 09:55 < genehacker> done 10:00 < genehacker> I love opera 10:01 < genehacker> well i am now hooked 10:02 -!- kardan| [n=kardan@p54BE4E37.dip.t-dialin.net] has quit [Read error: 110 (Connection timed out)] 10:04 < genehacker> no extensions though 10:21 < kanzure> genehacker: configure it to make tabs on the left-hand side in a readable list instead of at the top 10:21 < kanzure> and then you're all set to go. 10:21 < kanzure> also, with a multi-monitor setup you can devote a monitor to bookmarks or something, since the bookmark manager is kind of spiffy 10:29 < nsh> trust kanz 10:29 < nsh> he knows bookmarking 10:36 < kanzure> Thermodynamic constraints on neural dimensions, firing rates, brain temperature and size 10:36 < kanzure> http://arxiv.org/abs/0905.3690 10:41 -!- kardan| [n=kardan@p54BE4E37.dip.t-dialin.net] has joined #hplusroadmap 10:53 < kanzure> cis-action: hey 11:21 -!- kardan| [n=kardan@p54BE4E37.dip.t-dialin.net] has quit [Read error: 110 (Connection timed out)] 11:38 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has joined #hplusroadmap 11:40 < kanzure> how close do you need to get two molecules before a chemical reaction occurs? isn't there some curve for different subatomic forces that are acting on molecules that describes how r is always in the denominator or something? 11:43 -!- xp_prg [n=xp_prg3@98.234.52.78] has quit ["This computer has gone to sleep"] 12:11 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has quit [Read error: 110 (Connection timed out)] 12:17 -!- xp_prg [n=xp_prg3@99.2.31.217] has joined #hplusroadmap 12:25 < genehacker> kanzure mechanosynthesis? 12:25 < kanzure> ping 12:25 < kanzure> http://heybryan.org/ 12:26 < kanzure> does this work for anyone 12:26 < genehacker> it loaded 50 milliseconds longer than usual 12:26 < genehacker> it works 12:28 < genehacker> can I get some adblock stuff for opera? 12:28 < genehacker> btw how do browser companies make money? 12:29 < kanzure> opera sells itself to nintendo/wii/cellphones 12:29 < kanzure> there's no adblock for opera :( 12:31 < genehacker> oh 12:31 < genehacker> what about firefox 12:31 < genehacker> ? 12:31 < kanzure> it's free/open source software 12:31 < genehacker> they sell t-shirts I presume? 12:32 < kanzure> Mozilla, the foundation that primarily develops firefox, gets money from Google for putting them as the primary search 12:32 < kanzure> yeah, they sell lots of crap too, but they are largely a non-profit 12:32 < kanzure> so they also work off of donations etc. 12:32 < genehacker> oh 12:32 < genehacker> so that's how 12:32 < genehacker> always wonder 12:32 < genehacker> ed 12:32 < kanzure> well people like me improve firefox for free 12:33 < kanzure> that's what's so great about open source .. people work on it, and then stuff happens 12:33 < genehacker> anyway I need a way to make phosphoramidite nucleosides 12:33 < kanzure> genehacker: you should read more papers from acs.org (american chemical society) about DNA synthesis so that you can get a feel for what the fuck the chemistry is all about 12:34 < genehacker> the papers say how to make the photolabile base things 12:34 < kanzure> there are possibly some simpler reactions for synthesizing DNA that are out there 12:34 < genehacker> here's the deal 12:34 < kanzure> usually the chemical reactions for just about anything are available somewhere via ACS :/ 12:34 < xp_prg> genehacker I am excited by your idea 12:34 < xp_prg> ! 12:34 < genehacker> phosphoramidite nucleosides cost a lot 12:34 < genehacker> do you know why? 12:34 < genehacker> they are really high purity 12:34 < genehacker> did you read my diybio post? 12:35 < kanzure> who are you asking 12:35 < xp_prg> yes I did genehacker 12:35 < genehacker> xp_prg 12:35 < genehacker> so now you understand my evil plan 12:35 < kanzure> ok 12:35 < kanzure> http://heybryan.org/books/papers/ 12:35 < kanzure> it's back up 12:36 < genehacker> so I'd like to know how they get high purity stuff 12:36 < xp_prg> genehacker it sounds way cool! 12:36 < genehacker> from what I hear, they use chromatography 12:36 -!- kardan| [n=kardan@84.190.59.217] has joined #hplusroadmap 12:37 < genehacker> it's cool and I believe would could make a DNA synthesizer, we just need to find a way to get the chemicals cheaply so it doesn't cost $100 each time we run it 12:38 < kanzure> genehacker: did you know that your email to diybio was so poorly formatted? 12:38 < genehacker> what? 12:38 < genehacker> how so? 12:39 < kanzure> it had line breaks after every other word 12:39 < kanzure> makes it kind of hard to read 12:39 < genehacker> well I did copy and paste from notepad 12:39 < kanzure> ah, that's why 12:39 < xp_prg> genehacker so you going to make it? 12:39 < kanzure> you need to enable wordwrapping or something 12:39 < kanzure> before you paste 12:39 < kanzure> er, disable wordwrapping I mean 12:40 < genehacker> WHOA 12:40 < genehacker> WTF happened? 12:41 < genehacker> it looked all nice when it posted it in there? 12:41 < kanzure> well it's because you had line breaks 12:41 < kanzure> when gmail sends out email, it splits it up into lines of 72 characters or something 12:42 < kanzure> but if you have a newline character after 72 characters, then the next line will just have "the stuff that you want to .." 12:42 < genehacker> ok can I edit my post? 12:42 < kanzure> no 12:42 < kanzure> I suggest resending it 12:42 < genehacker> remove and repost? 12:42 < kanzure> there is no "remove" 12:43 < kanzure> you can try sending it to yourself first if you want to see how it will look 12:44 < genehacker> there is a remove 12:44 < kanzure> where 12:46 < genehacker> on options 12:46 < kanzure> the "report abuse" thing? 12:46 < kanzure> that's for spam. 12:46 < genehacker> no it's not 12:48 < genehacker> reposted 12:48 < genehacker> ugh 12:48 < genehacker> http://groups.google.com/group/diybio/browse_thread/thread/f44fa4989fa799ab 12:49 < genehacker> is that somewhat readable? 12:49 < genehacker> I emailed it to my self and it had spaces.. 12:51 < xp_prg> genehacker you going to make this thing? 12:51 < genehacker> if I can get the chemicals to make it work 12:51 < genehacker> yes 12:52 < kanzure> I need to hop a bus. 12:52 -!- kanzure [n=bryan@cpe-66-68-183-235.austin.res.rr.com] has quit ["leaving"] 12:56 < ultraleibniz> gah. campbell! 12:56 * ultraleibniz sets everything up again. 13:04 < genehacker> is leibniz a computer? 13:04 < ultraleibniz> yes 13:04 < ultraleibniz> http://en.wikipedia.org/wiki/Leibniz 13:04 < genehacker> yeah I know] 13:04 < genehacker> I need to consider working for the ADL... 13:04 < ultraleibniz> it won't work out this summer 13:04 < ultraleibniz> he already hired all of the undergrads that he wants to 13:05 < genehacker> oh that's ok 13:05 < ultraleibniz> however, he was interested in your super microfluidic synthesize-the-shit-out-of-anything design 13:05 < genehacker> writing graph grammars for organic chemistry? 13:05 < ultraleibniz> yeah 13:05 < ultraleibniz> and also probably something involving a component library for the unit processes 13:05 < genehacker> I don't know that much about organic chemistry... 13:05 -!- kanzure [n=bryan@cpe-66-68-183-235.austin.res.rr.com] has joined #hplusroadmap 13:06 < genehacker> heh make the chem e 13:06 < genehacker> s obsolete 13:06 < kanzure> did you see the retrosynthetic route designer paper thingy? 13:06 < ultraleibniz> http://heybryan.org/books/papers/Route%20designer%20-%20a%20retrosynthetic%20analysis%20tool%20utilizing%20automated%20retrosynthetic%20rule%20generation.pdf 13:07 < kanzure> drazak_: can you find me any references on physical imprints of the size/shape of different nucleotides? 13:10 < kardan|> hi, whats a AFM cantilever? 13:11 < kardan|> (just read some pages back) 13:11 < kanzure> do you know what AFM is? 13:11 < kanzure> AFM is atomic force microscopy- one of the forms of analytical instrumentation- 13:12 < kanzure> http://heybryan.org/instrumentation/instru.html 13:12 < kanzure> where you use a super-fine tipped needle to poke a surface 13:13 < kanzure> using a very flimsy reflective 'cantilever', or 'long rod looking thingy' you can detect how much the reflective film bends due to force on the super-fine tipped needle 13:13 < kanzure> you can detect this by using a laser shinning at the cantilever. based off of the angle of deflection, you can figure out how much force is being exerted on your tip. 13:13 < kanzure> you detect the angle of detection via (typically) a four-segmented photodiode 13:13 < kanzure> although there are some that use CMOS image sensors or whatever 13:15 < kanzure> er, angle of deflection (sorry) 13:15 < kardan|> thats what i am interested too 13:15 < kanzure> are you the one that doesn't speak english? 13:16 < kanzure> :p 13:23 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has quit [Read error: 110 (Connection timed out)] 13:48 < bluekanzure> "username 'kanzure' is already registered on arxiv.org" 14:14 -!- wrldpc [n=worldpea@pool-173-48-214-204.bstnma.fios.verizon.net] has joined #hplusroadmap 14:20 -!- kardan| [n=kardan@84.190.59.217] has quit [Read error: 110 (Connection timed out)] 14:28 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has joined #hplusroadmap 15:20 < kanzure> On Tue, May 26, 2009 at 3:00 PM, BillK wrote: 15:20 < kanzure> > If you don't have all the legally required animal testing licenses, 15:20 < kanzure> > premises inspections, animal care permits, etc. then what you are 15:20 < kanzure> > doing is torturing animals for a hobby. 15:25 < Utopiah> watch out, in few days you might have hot celebrities from PETA pounding on your door... 15:26 < kanzure> do I get to bone them? 15:28 < Utopiah> only if you don't label that action "animal experiment" 15:28 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has quit [Connection timed out] 15:28 < Utopiah> (starting to sound a bit awkward) 15:29 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has joined #hplusroadmap 15:33 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has joined #hplusroadmap 15:57 < kanzure> > But what value do you think such research will have? 15:57 < kanzure> > 'Here, take these pills. A friend of mine fed them to some rabbits in 15:57 < kanzure> > Indonesia and they seemed to be stronger than usual'. Really??? 16:07 -!- mindspillage [n=kat@wikimedia/KatWalsh/x-0001] has quit [Read error: 113 (No route to host)] 16:52 < genehacker> WOOHOO 16:53 < genehacker> I have a damn tiny water pump! 16:55 < kanzure> a tiny wong? 16:56 < genehacker> no 16:57 < genehacker> I'm making a shirt with a heat exchanger 16:58 < genehacker> I needed a pump that would be small yet powerful enough to drive coolant through ~15 feet of aquarium tubing so I don't have to be hot in the summer 16:58 < genehacker> So I found a $5 motorized water gun 16:59 < fenn_> where did you find that? 16:59 -!- fenn_ is now known as fenn 16:59 < genehacker> happens to have a fitting that fits the aquarium tubing, on one end... 16:59 < genehacker> Toys R Us 17:00 < genehacker> went all over town looking for something like that 17:00 < genehacker> hmmm... 17:00 < genehacker> wonder if that pumps strong enough to drive my flamethrower... 17:00 -!- duzt [n=duzt@dsl093-216-054.aus1.dsl.speakeasy.net] has joined #hplusroadmap 17:02 < fenn> is it just me or are squirt guns steadily progressing towards 'covenant plasma rifle'? http://rsk.imageg.net/graphics/product_images/pRS1C-2305387w345.jpg 17:10 < genehacker> it is but just barely 17:10 < genehacker> it is strong enough but my flamethrower is a bit leakyier than anticipated 17:11 < genehacker> it is not just you 17:13 < genehacker> http://www.amazon.com/gp/product/B000LZFTY6/ref=s9_simx_gw_s0_p21_t2?pf_rd_m=ATVPDKIKX0DER&pf_rd_s=center-2&pf_rd_r=00K0WBGFZHZ3EJ70VN0R&pf_rd_t=101&pf_rd_p=470938631&pf_rd_i=507846 17:13 < genehacker> http://halo3blogs.files.wordpress.com/2009/03/plasmarifle-halo.jpg 17:13 < fenn> the wikipedia article on water guns is pretty awesome 17:14 < genehacker> I'm document this stuff 17:14 < genehacker> I'm going to document this pump 17:15 < genehacker> a water gun that uses a peristaltic pump? 17:15 < genehacker> interesting 17:16 < fenn> it's only to be expected 17:16 < fenn> white tubing? about 1/8"? 17:16 < genehacker> 0.17 inch 17:17 < genehacker> also this watergun was not meant to be dissassembled 17:17 < genehacker> part of the plastic was ultrasonically welded 17:18 < fenn> try using a brazilian screwdriver 17:25 < genehacker> a crow bar? 17:25 < genehacker> I just use my bear hands 17:27 < fenn> i didnt know you were a raccoon 17:29 < fenn> this is much harder to make it work than it seems: http://en.wikipedia.org/wiki/Assassin_(game) 17:30 < genehacker> article = dead 17:35 -!- genehacker_ [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has joined #hplusroadmap 17:36 < genehacker_> you're already registered in arxiv?' 17:36 < fenn> genehacker_: what article is dead? 17:36 < genehacker_> the assasin game 17:37 < fenn> copy and paste the link 17:38 < genehacker_> what is a brazilian screwdriver? 17:38 < genehacker_> a knife 17:40 < fenn> a hammer 17:40 < genehacker_> oh 17:40 < fenn> ... i think. i doubt anybody really knows 17:46 < fenn> how come texas doesnt have anything like this? http://en.wikipedia.org/wiki/Songkran 17:52 -!- genehacker [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has quit [Read error: 110 (Connection timed out)] 17:53 < genehacker_> we do 17:53 < genehacker_> acouple months ago with water balloons and colored powder instead of chalk 17:54 < genehacker_> that was the indian students association thing though 17:54 < genehacker_> so I don't know really 17:54 < fenn> it's not hot here in april though 17:54 -!- genehacker_ [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has left #hplusroadmap [] 17:54 -!- genehacker_ [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has joined #hplusroadmap 17:54 < genehacker_> oops 17:54 < genehacker_> what did I do? 17:55 < fenn> you used a web browser 17:55 < genehacker_> no 17:55 < genehacker_> I hit one of the exit keys 17:56 -!- nsh [i=sbp@wikipedia/nsh] has quit [Read error: 110 (Connection timed out)] 17:57 < fenn> genehacker_: do you know how to use ssh? 17:57 < genehacker_> no 17:58 < genehacker_> tell me 17:58 < genehacker_> hmmm 17:58 < genehacker_> pump is an impellor type 17:58 < fenn> er, well on a windows machine you'd download putty.exe 17:58 < genehacker_> ok 17:59 < fenn> nevermind, i'm just wondering why my mail server is rejecting ssh connections 18:17 -!- dizt [n=duzt@dsl093-216-054.aus1.dsl.speakeasy.net] has joined #hplusroadmap 18:18 < drazak_> kanzure: what do you want me to look up? 18:21 < kanzure> drazak_: how to make a hole in which only a certain nucleotide can become stuck in 18:22 < drazak_> could you use part of the substrate section from a reverse transcriptase? 18:22 < kanzure> drazak_: just so I get things straight, your name is ben too? 18:22 < drazak_> yeah 18:23 < kanzure> why is this channel so heavily b-biased when it comes to letters of first names 18:23 < drazak_> b for brain 18:23 < kanzure> drazak_: possibly, if you could find one of those papers that studies the angstrom-scale structure via x-ray crystallography or something, it might be a start 18:23 < drazak_> yeah 18:23 < drazak_> stupid java application 18:24 < drazak_> won't let me alt tab ut of it 18:24 < kanzure> "help! I'm trapped in a java applet!" condolences. 18:24 < kanzure> yay for Austin Che 18:24 < kanzure> http://www-lecb.ncifcrf.gov/~toms/latex.html 18:25 < drazak_> wtf 18:25 < drazak_> isn't makedir the command to make a folder? 18:25 * drazak_ losing it 18:25 < kanzure> mkdir 18:25 < drazak_> thanks 18:25 < kanzure> now you must sacrifice the gods 18:25 < drazak_> I'm just trying to get music onto my fucking mp3 player 18:25 < drazak_> it's a bitch 18:25 < kanzure> *to the gods 18:26 < drazak_> it won't transfer by usb because it's half broken, so I have to plug the base into the network 18:26 < fenn> sounds like it needs an exorcism 18:27 < kanzure> apple be gone? 18:27 < fenn> the worm that ate the apple 18:27 < drazak_> it's a rio karma 18:28 < fenn> ah, well if it's karma then there's nothing we can do, since obviously you deserve it 18:28 < kanzure> fenn: re: lisp stuff, I've continued to just comment the living hell out of the file and it seems to be making me think I'm making progress 18:28 < kanzure> whether or not there actually is any progress only time will tell 18:28 < kanzure> er, debug-print-statement-style comments 18:28 < fenn> well, here's how you know if you have someting useful 18:29 < kanzure> if it works? 18:29 < fenn> if you can feed it data and it spits out te result of some calculation 18:29 < kanzure> right. 18:29 < fenn> for example, f(x) = x^2 18:29 -!- splicer [n=patrik@h177n3c1o261.bredband.skanova.com] has joined #hplusroadmap 18:30 < fenn> hullo there splicer 18:30 -!- duzt [n=duzt@dsl093-216-054.aus1.dsl.speakeasy.net] has quit [Read error: 110 (Connection timed out)] 18:30 < genehacker_> now where do I get 5/8 in diameter pipe? 18:30 < genehacker_> od of course 18:30 < splicer> hi fenn 18:31 < kanzure> the fact that you have to ask "where do I get this standard-sized part" tells you that something is wrong with the system 18:31 < fenn> ey i had this idea too (probably aroun te same time, must be te hylaean theoric wave front) http://www.ccrnp.ncifcrf.gov/~toms/patent/molecularrotationengine/ 18:31 < genehacker_> yeah 18:31 < kanzure> is that the US obfuscation organization agency? 18:31 < drazak_> finally 18:31 < drazak_> got it transfering 18:32 < drazak_> but I think the base is only 10mbps 18:32 < fenn> kanzure: no it's a theory of why everyone comes up with te same tings at the same time 18:32 < genehacker_> is that a brownian rectifier? 18:32 < kanzure> fenn: no, the TLD/domain name in the URL. 18:32 < kanzure> ccrnp ncifcrf gov 18:32 < fenn> it's a muscle fiber powered rotary engine 18:32 < genehacker_> WHOA 18:33 < kanzure> ah neat 18:33 < kanzure> I'm glad somebody has done this 18:33 < genehacker_> I wonder if this is more efficient than muscle 18:33 < kanzure> how much power do you get out 18:33 < genehacker_> I wonder if one was ever built 18:33 < drazak_> any of you dudes going to be in boston over the summer? 18:33 < genehacker_> muscle is about 25% efficient 18:33 < fenn> drazak_: i wish 18:34 < kanzure> drazak_: I will be there in spirit 18:34 < drazak_> I might be a research assistant in boston 18:34 < drazak_> for my uncle 18:35 < fenn> where does your uncle work? 18:35 < drazak_> uh 18:35 < drazak_> research at harvard 18:35 < kanzure> isn't that cheating 18:36 < drazak_> a little :P 18:36 < drazak_> narcissism is horrible until it happens to you! 18:36 < fenn> nepotism 18:36 < drazak_> whatever 18:36 < splicer> hehe 18:36 < fenn> nihilism 18:36 < genehacker_> hmmm... 18:37 < genehacker_> I wonder if this pump would be submersible 18:37 * drazak_ nihilist 18:38 < drazak_> kanzure: what was the enzyme called? 18:38 < genehacker_> GASP-1? 18:38 < kanzure> for what 18:38 < drazak_> kanzure: the one ofr cleaving da 1 nucleotide at a time 18:38 < kanzure> it's related to an endonuclease 18:38 < kanzure> not a ligase .. it's a... er.. 18:39 < kanzure> line 18:39 < drazak_> fenn: were you there when I talked to him last night? 18:39 < fenn> hether for art thou, romeo 18:40 < fenn> drazak_: yes 18:40 < drazak_> what enzyme did he say? 18:40 < fenn> i wasn't really listening 18:40 < kanzure> have I been making up enzymes again? 18:40 < drazak_> maybe 18:40 < kanzure> I could have sworn this exists 18:40 < kanzure> you could use an endonuclease to cleave off one nucleotide if you attach the cleavage site to it 18:41 < drazak_> ok 18:41 < drazak_> I'll just say that 18:41 < drazak_> I'm trying to further impress my uncle so that he'll take me 18:41 < drazak_> :P 18:41 < fenn> "A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids." 18:42 < kanzure> drazak_: the real way to impress him is to read all of his papers and cite it in your proposal or something 18:42 < kanzure> what is his name / link? 18:42 < fenn> Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, in contrast to exonucleases, which cleave phosphodiester bonds at the end of a polynucleotide chain. 18:42 < drazak_> kanzure: e finkelstein 18:43 < kanzure> seriously? 18:43 < drazak_> yes? 18:43 < kanzure> hehe 18:43 < drazak_> you know his papers? 18:43 < drazak_> he works on lab on a chip stuff 18:44 < kanzure> hm. 18:44 < kanzure> no. 18:44 < drazak_> he married into the family 18:44 < fenn> i dont see any E. Finkelsteins 18:44 < drazak_> I'm not a finkelstein 18:44 < kanzure> the results on google scholar are nil 18:44 < kanzure> does this guy exist? 18:45 < drazak_> hold on 18:45 < splicer> https://www.researchgate.net/author/E+Finkelstein 18:45 < drazak_> he goes by eb finkelstein 18:45 < drazak_> I thought he did e finkelstein 18:45 < kanzure> I only see: The role of VEGF isoforms in tumor angiogenesis 18:46 < drazak_> yeah 18:46 < drazak_> I saw that one too 18:46 < drazak_> when he was working in syracuse 18:46 < drazak_> his stuff is like darpa now 18:46 < drazak_> so you might have to search patents 18:46 < kanzure> urhm. 18:46 < drazak_> http://www3.interscience.wiley.com/journal/104084493/abstract 18:46 < fenn> yay SCIENCE 18:47 < kanzure> fenn: do you have a UT login yet? 18:47 < drazak_> http://www.informaworld.com/smpp/content~content=a713811278~db=all 18:47 < kanzure> http://ezproxy.lib.utexas.edu/login?url=guess-what 18:47 < fenn> kanzure: sort of; they wont let me use it for anything 18:47 -!- samrose [n=samrose@c-24-11-214-181.hsd1.mi.comcast.net] has quit ["Ex-Chat"] 18:47 < kanzure> fenn: surely they will let you use the library's online services? 18:48 < fenn> oh, wow 18:49 < fenn> ok how do i use this 18:49 < kanzure> did it log you in? 18:49 < kanzure> it's just a proxy, so it does a search-and-replace on all URLs or something 18:50 < kanzure> and lets you access PDFs from the super-evil journals 18:50 < drazak_> I know my friends university access 18:50 < drazak_> I can use it to search 18:51 < kanzure> I wish I would have had a friend back in the day who gave me university access to scholarly literature 18:51 < kanzure> back in high school I had to steal it from the taliban or something 18:51 < fenn> i got a pdf! 18:51 < drazak_> rofl 18:51 < kanzure> go pokeball! 18:51 < drazak_> I lost it though 18:51 < drazak_> :( 18:52 < fenn> good old taliban password bbs'es 18:52 < fenn> we would trade opium and RPG's for journal articles 18:52 < drazak_> haha 18:53 < splicer> maybe they are 18:55 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has quit [Read error: 110 (Connection timed out)] 18:55 < splicer> is it true that lab assistants are underpaid? 18:55 < kanzure> yes 18:56 < fenn> how could it not be true? 18:57 < drazak_> I'll probably work for free 18:57 < drazak_> I'd be staying with him and my aunt in boston 18:57 < splicer> somehow i thougt it was a bit like the software business... not that most people make a lot of money.. but they get by at least. 18:57 < fenn> drazak_: make sure to go spy on the media lab for us 18:57 < genehacker_> TELL US EVERYTHING 18:57 < drazak_> I'm going to spy on the whole thing 18:57 < drazak_> they're growin HEARTS 18:57 < fenn> and MINDS 18:57 < drazak_> and BONES 18:57 < drazak_> and KIDNEYS 18:58 < genehacker_> I could use a second heart 18:58 < genehacker_> that way I could be just like doctor who 18:58 < drazak_> lol 18:58 < genehacker_> always build in redundancy... 18:59 < fenn> sigh.. all my old muds are wiped from the face of the internet 18:59 < splicer> where would you put it? 18:59 < drazak_> and he's growing skeletal muscle 19:00 < fenn> genehacker_: and make it run on a fusion reactor core so you dont have to recharge it every twenty minutes 19:00 < kanzure> is it wrong that I've played more MOOs than MUDs? :( 19:00 < genehacker_> now how I'm I supposed to build a handheld switch and battery case that doesn't look like a trigger to a suicide bomber vest? 19:00 < kanzure> just give it curves 19:00 < fenn> it's mostly a matter of which one you were exposed to first, i think 19:01 < genehacker_> deabbriviate those acronyms plz 19:01 < kanzure> fenn: unfortunately, I was exposed to neither 19:01 < fenn> either one will get you humiliated in public though, so i dont really see the difference 19:01 < kanzure> but for some reason I said hello on the Artemis Society MOO server 19:01 < fenn> MOO is more interactive 19:01 < kanzure> ah, that's right, MUDs had more backend servery goodness 19:01 < fenn> MUD is more .. solid 19:02 < genehacker_> That's it 19:02 < fenn> sort of like secondlife vs WoW 19:02 < genehacker_> I'll use a reed switch 19:02 < genehacker_> oh 19:02 < genehacker_> deabriviation plz? 19:02 < fenn> multi user dungeon/dimension, MUD object oriented (i guess) 19:02 < fenn> it doesnt really elp does it 19:03 < fenn> an IRC channel is like one 'room' or grid coordinate in a virtual mud world 19:03 < fenn> but on IRC we can only talk and emote (/me) 19:04 < fenn> whereas in a mud there are also items and actions and state variables like hitpoints etc 19:04 < kanzure> er, wait, did MUDs do IRC integration? 19:04 < drazak_> lol 19:04 < kanzure> it was like interactive fiction on steroids 19:04 < fenn> i wouldnt be surprised, but usually not 19:04 < kanzure> IIRC 19:04 < drazak_> this is takin for fucking ever 19:04 < fenn> there was a mud-wide IRC channel, channel for each clan or guild or whatever 19:04 < fenn> er, except it wasnt IRC 19:04 < fenn> the protocol i mean 19:05 < fenn> i think my mind was permanently shaped by early exposure to muds 19:05 < fenn> and that's why i feel comfortable with a command line (it's how i learned to type after all) 19:06 < splicer> people used to use muds as irc:s 19:06 < fenn> VieMUD was heavily influenced by unix concepts 19:06 < fenn> you could do variable substitution, set up aliases, shell history search, etc 19:06 < fenn> and of course tab completion 19:07 < kanzure> huh 19:07 < kanzure> sounds nice. 19:07 < kanzure> sadly I learned to roleplay on forums .. not quite the best place. 19:07 < fenn> my condolences 19:07 < kanzure> must increase post count .. 19:07 < kanzure> to get to level 2.. 19:07 < kanzure> blah. 19:07 < genehacker_> I don't roleplay 19:08 < fenn> anyway viemud was written in plain C and suffered under the decades of code hacking 19:08 < fenn> eventually it was down to just one Implementor 19:08 < kanzure> much like nethack? 19:08 < kanzure> heh 19:08 < fenn> nethack is gross 19:08 < fenn> more like interactive storytelling 19:08 < kanzure> fenn: I introduced you to Bill at The Castilian. he just sent me a link over to this feynman/connection-machine article: http://www.longnow.org/views/essays/articles/ArtFeynman.php 19:08 < fenn> but with guns and swords 19:08 < kanzure> nice to see someone I've known since high school reading longnow.org 19:09 < genehacker_> I like nethack 19:09 < kanzure> you play nethack? 19:09 < genehacker_> I played it a little 19:09 < genehacker_> I have it on my DS 19:09 < kanzure> wtf 19:09 < genehacker_> but it doesn't work with my flashcart 19:09 < genehacker_> ever hear of homebrew? 19:10 < kanzure> yeah 19:10 < kanzure> of course .. we've all done our own gameboy color games at one point or another 19:10 < genehacker_> I haven't yet... 19:10 < kanzure> hex hacking nastolgia. 19:10 < fenn> it's really kind of amazing how many awesome ideas people have ad in the past that were just too early 19:10 < genehacker_> too much freestuff 19:10 < fenn> stuff like transputer/connection machine, or engelbart's stuff 19:10 < genehacker_> too little freetime 19:11 < genehacker_> who is this bill person? 19:11 < kanzure> high school friend 19:12 < drazak_> jesus fuck 19:12 < kanzure> he's smart, but not in the way that makes him do anything 19:12 < drazak_> this album is a fucking gig 19:12 < kanzure> but he doesn't do nothing in the same sense that I do nothing 19:12 < kanzure> he seems to just play guitar or something :p 19:13 < genehacker_> well he might be useful 19:13 < genehacker_> one of my friends who builds ridiculous custom nerf guns also happens to play guitar 19:13 < fenn> heh "Instead, we planned to connect the processors in a 20-dimensional hypercube" 19:13 < kanzure> if you want to talk with him, he's bluesplayer@gmail.com 19:13 < genehacker_> he's trying to make his own dart factory 19:14 < kanzure> OR! a 20-d TIMECUBE! yeah! 19:14 < genehacker_> haha time cube 19:14 < genehacker_> The time cube ⬖ ⬗ ⬘ ⬙ 19:14 < genehacker_> THE GOVERNMENT ENCODED IT INTO UNICODE 19:15 < kanzure> isn't unicode ISO, and not US govt? 19:15 < fenn> i, er, yeah 19:15 < splicer> kanzure: Have you directed a swedish transhumanist named Alexander my way? 19:15 < kanzure> splicer: no, I don't think so 19:15 < splicer> k.. thanks 19:15 < kanzure> splicer: btw, do you know Anders Sandberg yet? 19:15 < fenn> there was someone in here trying to start wta.se? or transhumanism.se maybe 19:15 < kanzure> http://aleph.se/ 19:15 < splicer> yeah... alexander wanted transhumanism.se 19:15 < fenn> or maybe e was trying to make transhumanism.se more general and less biohacking focused 19:16 < splicer> so sandberg was here? 19:16 < kanzure> it's sad that Anders is just an ethicist at Oxford now 19:16 < kanzure> no, sandberg never talks with me 19:16 < splicer> i don't know any of them 19:19 < fenn> them? 19:19 < splicer> kanzure is the only one who sometimes calls himself transhumanist i know 19:20 < kanzure> I'd rather be a transhuman than an -ist :( 19:20 < splicer> i think their time has passed... but thats another topic 19:21 < splicer> i think it was probably great to be an extropian in the 80:ies 19:21 < fenn> pfff 19:21 < kanzure> what did they accomplish? 19:21 < fenn> the 2080's maybe 19:22 < fenn> if we're lucky 19:22 < splicer> they were a think tank... they had a lot of fun 19:22 < fenn> kanzure: they wrote a bunch of books 19:22 < fenn> and had some newsgroup flame wars 19:22 < fenn> about the same as now, pretty much 19:23 < splicer> it was the time to have flamewars 19:23 < kanzure> I should start using history as an example 19:23 < kanzure> "how is this any different from the 80s" 19:23 < fenn> it just seems so much more grand because we get a whole decade compressed into a single wiki article 19:26 < splicer> the garage biology culture needs to get started... we need the new apples and hp:s 19:26 < kanzure> how can we make sure that doesn't happen to me 19:26 < kanzure> splicer: nobody cares enough to listen to us 19:26 < kanzure> everybody's too busy talking about the ethics of biohacking and other bullshit 19:26 < kanzure> rather than actually fixing any of the problems they are talking about 19:26 -!- nsh [n=nsh@host86-141-178-190.range86-141.btcentralplus.com] has joined #hplusroadmap 19:27 < splicer> yup... it's like everyone thinks there are scientists in white lab coats taking care of business. 19:27 < genehacker_> well that's what I'm trying to do with my DNA synth project 19:28 < genehacker_> no response in diybio 19:28 < kanzure> nobody has replied to you 19:28 < genehacker_> because of the horrible formatting? 19:29 -!- kardan| [n=kardan@p54BE3BD9.dip.t-dialin.net] has joined #hplusroadmap 19:29 < kanzure> the second time you sent it, it looked better 19:29 < kanzure> so now it's just because people are lazy fucks 19:29 < bkero> kanzure: yo 19:29 < genehacker_> still it's a big pile of words 19:29 < genehacker_> maybe if I make it easier to read 19:30 < genehacker_> I'll get a response 19:30 < fenn> genehacker_: i'm pretty certain you'll have to build a working model before anyone gets excited 19:30 < genehacker_> I need some damn phosphoramidite nucleosides! 19:30 < genehacker_> ok 19:30 < fenn> so steal some 19:31 < genehacker_> hmmm 19:32 < genehacker_> so first I need to find out if they have a DNA synth here on campus, then I have to find a way to disable the alarm system... 19:32 < kanzure> ellington has one 19:32 < genehacker_> he does 19:32 < genehacker_> hmmm... 19:32 < kanzure> do you want the operating manuals? 19:32 < genehacker_> nah I can find those online 19:32 < kanzure> how could you if you don't know the model? 19:32 < genehacker_> but if you have em in PDF form send them to me 19:32 < fenn> i suspect the nucleosides will be in bottles in a cabinet next to the macine 19:33 < kanzure> or in the refirdgerator 19:33 < genehacker_> because I already found one 19:33 < splicer> they are expensive i think 19:33 < kanzure> refrigerator 19:33 < kanzure> you found one what? 19:33 < genehacker_> they will be in four different bottles connected to the machine 19:33 < kanzure> not if they aren't loaded. 19:33 < kanzure> also, it's more than four bottles 19:33 < kanzure> those are the dNTPs 19:33 < genehacker_> they tend to stay loaded 19:34 < genehacker_> what is that? 19:34 < kanzure> nucleotides. 19:34 < genehacker_> perhaps we could borrow some 19:34 < genehacker_> legitly borrow some 19:34 < fenn> sort of like borrowing a cup of sugar 19:35 < kanzure> http://heybryan.org/books/ellingtonlab/A_E-Lab_Stuff/Software/Programs/ 19:35 < fenn> you have to say it like 'pardon me, do you have any grey poupon' 19:36 < genehacker_> if we could make a microfluidic instant DNA synth we could ask for about 5 ml and we might be set for life 19:36 < kanzure> eh 19:36 < kanzure> you apparently don't know about debugging yet 19:37 < genehacker_> yeah... 19:37 < genehacker_> I wonder how much stuff is used up each nucleotide cycle with the maskless litho thing? 19:39 < fenn> depends how much you add 19:39 < fenn> higher concentration = faster reaction kinetics 19:39 < kanzure> why not vary temperature, pressure, concentration, etc. 19:40 < kanzure> I don't know why I don't see more experiments done under high pressure or something 19:40 < kanzure> wouldn't it be advantageous? 19:40 < fenn> temperature might cause bad side reactions like depolymerization 19:40 < fenn> pressure is expensive and somewhat dangerous 19:40 < genehacker_> huh 19:41 < fenn> you have to get into GPa to really see weird cemical reactions 19:41 < kanzure> not in a microfluidic system. you could apply pressure by turning a screw, or putting a giant brick on it or something 19:41 < fenn> unless they're gas phase reactions 19:41 < genehacker_> that is interesting because take a look at this: 19:41 < kanzure> fenn: GPa is probably easier to reach in smaller volumes 19:42 < fenn> of course 19:42 < drazak_> deacronym 19:42 < fenn> giga pascal = 145000 psi 19:42 < drazak_> oh 19:42 < drazak_> psi no help, but ok 19:42 < fenn> pounds per square inc 19:42 < genehacker_> hold on 19:42 < kanzure> I've sometimes thought about a method to make GPa or kPa at least with your finger force 19:42 < kanzure> but in sufficiently small volumes or something 19:42 < drazak_> I know what psi IS 19:43 < drazak_> oh right 19:43 -!- nsh [n=nsh@host86-141-178-190.range86-141.btcentralplus.com] has quit [Remote closed the connection] 19:43 < genehacker_> point is in the dna chips by maskless lithography patent, the reaction chamber looks like it is built for high pressure 19:43 < drazak_> 101.7kpa=atm gigapascal=10000atm 19:43 < genehacker_> or that it was over engineered 19:44 < fenn> what makes you say that 19:45 < genehacker_> well when I find it you'll see 19:46 -!- fenn_ [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has joined #hplusroadmap 19:46 < fenn_> gah 19:46 -!- fenn [n=fenn@cpe-72-177-52-180.austin.res.rr.com] has quit [Nick collision from services.] 19:46 -!- fenn_ is now known as fenn 19:47 < fenn> so you were making empty threats about your dna synthesizer 19:47 < splicer> there is probably a very elegant dna synthetization method just waiting to be discovered 19:47 < splicer> somewhere 19:47 < fenn> i have it 19:47 < splicer> the light thing? 19:48 < fenn> yes 19:48 < fenn> could it get any more elegant? 19:48 < splicer> you may be right 19:48 < genehacker_> blast 19:48 < fenn> if a mechanical computer were integrated somehow, peraps (not more elegant but infinitely more useful) 19:48 < genehacker_> I'm having trouble finding it 19:49 < genehacker_> mechanical integrators? 19:49 < genehacker_> they exist 19:49 < genehacker_> mechanical differential integrators 19:49 < fenn> mechanical computer + laser diodes + dna read/write head 19:49 < fenn> then you have a network between cells, eac cell is a network node 19:50 < genehacker_> maskless photolithography gene synthesis is pretty elegant 19:50 < fenn> laser link being te physical layer, sort of like wifi since you're below the diffraction limit 19:50 < fenn> then the computer could demultiplex single-frequency light, or other data transmission schemes 19:51 < fenn> instead of the write head being "hard wired" to write an A on 440 nm or whatever 19:52 < genehacker_> http://www.google.com/patents?id=YYIKAAAAEBAJ&dq=method+and+apparatus+for+synthesis+of+arrays+of+dna+probes 19:52 < fenn> there is someting hard stuck underneath my "h" key and i dont know how to get it out without breaking the key off 19:52 < genehacker_> take a look at this 19:52 < kanzure> fenn: laptop keyboards can be removed 19:52 < genehacker_> mechanical computers need to be made very accurately in order to be accurate 19:52 < fenn> yeas.. rmm but the laptop would probably disintegrate and i'll never get it back together 19:52 < splicer> fenn: the print head is a protein? 19:52 < fenn> splicer: yes, protein complex 19:53 < genehacker_> are laptop keyboards not supposed to be removable? 19:53 < kanzure> no, they are generally replaceable 19:53 < fenn> splicer: like PolIII but with some antennae stuck on it (in the hard wired version) 19:53 < genehacker_> Dell laptop keyboards are? 19:53 < kanzure> maybe. you should check the screws. 19:53 < splicer> fenn: checking 19:54 < genehacker_> see sheet seven on the patent 19:54 < genehacker_> that slide is bolted down 19:54 < genehacker_> oh 19:54 < genehacker_> for alignment 19:55 < fenn> genehacker_: what is the patent number? 19:56 < fenn> oh nm i see google now allows you to download a pdf 19:58 < fenn> splicer: the green and purple blob in http://www.youtube.com/watch?v=Y6FitehwJg4 19:58 < genehacker_> yeah 19:58 < genehacker_> which I why I like google patent 19:58 < fenn> splicer: you'd have to supply it with a fake template strand 19:59 < fenn> or else modify the enzyme someow so that the template strand won't interfere with bases in the new strand 19:59 < kanzure> conformational changes to make it only allow certain template nucleotides would be a nice way to go about it 19:59 < kanzure> then you immobilize the polymerase to an electrode or something 20:00 < fenn> and move the dna around? that would take forever 20:00 < kanzure> polymerase moves the dna 20:00 < splicer> fenn: thanks 20:00 < fenn> exponentially forever 20:00 < kanzure> er 20:00 < kanzure> polymerase moves it I thought? 20:00 < kanzure> is there any immobilized polymerase function studies out there? 20:00 < kanzure> I don't think I've seen any 20:00 < fenn> polymerase moves along the template strand like a train on train tracks 20:01 < fenn> yes, i'm sure there are 20:01 < kanzure> ok, so you don't have to move the strand then 20:01 < kanzure> the train doesn't move the planet :p 20:01 < kanzure> unless you're watching futurama 20:01 < fenn> the pacific biosciences sequencer works with an immobilized polymerase 20:01 < fenn> kanzure: generally DNA is much higher molecular weight than the polymerase molecule 20:02 < kanzure> bah, it's a strong 'lil fighter 20:02 < fenn> so i say the polymerase moves along the DNA 20:02 < fenn> now, in some PCR reaction it might be different 20:02 < fenn> but really who cares 20:03 < kanzure> I think an electrical method to change the conformation of an immobilized polymerase is somewhat more likely to work than engineering a laser/wavelength conformational system 20:03 < kanzure> if only because you can immobilize the polymerase and already have it at a specific location or something 20:04 < fenn> you still have to figure out how to make the correct conformational change, which i think is the hard part 20:04 < fenn> in a distributed amorphous system, i dont have to worry about attaching to a specific electrode 20:05 < fenn> i just have to make sure the floaty things are all stuck together 20:05 < genehacker_> cool 20:05 < fenn> and it's all bio-producible 20:05 < kanzure> maybe I should just go read up on protein engineering 20:05 < fenn> i mean, why would you want electrodes? 20:06 < genehacker_> I just saw some mammatus clouds 20:06 < genehacker_> electrodes? 20:06 < genehacker_> are you talking about the method of microarray DNA synthesis that uses electrodes 20:06 < kanzure> the azobenzene light-gated ion channels are a nice example 20:07 < kanzure> maybe a polymerase that has different tunnels for different nucleotides in the template would be ideal? 20:07 < kanzure> no 20:07 < kanzure> this is fenn's super writozyme thingy 20:07 < fenn> genehacker_: yes pretty much.. but the electrodes are the easy part 20:07 < fenn> and i think it'd work better without them anyway 20:07 < splicer> as I remember it inside the polymerase there is a mechanism that reads the nucleotides a bit ahead of time... so it's this mechanism that has to be modified? 20:08 < kanzure> fenn: be careful, the electrode methods are a different technique and might be what genehacker_ is talking about .. i.e., the methods that use acids 20:08 < kanzure> er, the acid/electrolysis versions or something 20:08 < fenn> oh 20:08 < fenn> well it woulnt be the first time he misunderstood 20:08 < genehacker_> there was a company made that uses some crazy electrochemical chips to synthesize DNA microarrays 20:08 < kanzure> see? 20:08 < genehacker_> chips full DNA strands 20:09 < fenn> kanzure: are there well understood (simple) models of protein conformational change in response to voltage/current? 20:09 < kanzure> fenn: voltage-gated ion channels? 20:09 < kanzure> I should hit you over the head for asking that question 20:09 < kanzure> :p 20:10 < fenn> wtf? ion channel isnt a conformational change 20:10 < kanzure> oh 20:10 < kanzure> er 20:10 < kanzure> it opens and closes in response to voltage. the closing is a blocking phenomena 20:10 < fenn> hrmm 20:10 < fenn> what makes it move? 20:10 < kanzure> hold on, doing some sys admin bullshit for the sata lab 20:11 < genehacker_> oh shoot 20:11 < genehacker_> I need to sign up for that 20:11 < fenn> splicer: i'm getting polymerase confused with old CNC paper tape machinery now.. are you sure there is readahead in polymerase? 20:12 < splicer> fenn: i'll check... i think there has to be 20:13 < splicer> in the back of my head i think it was like 10bases.. i'll check 20:13 < genehacker_> wouldn't a paper tape driven CNC be a ribosome? 20:13 < fenn> splicer: are you thinking of the mismatch detection? 20:14 < fenn> there's both pre- and post- error correction 20:14 < splicer> mmm.. yeah, 20:14 < splicer> i think you're right 20:14 < fenn> as far as i know the actual 'swap' occurs in one step 20:14 < fenn> there's no RAM or anything 20:14 < fenn> after all what would be the point? 20:14 < kanzure> http://heybryan.org/school/buildingbrains/all.html 20:14 < kanzure> search for "voltage-gated" or "voltage gated" 20:15 < kanzure> er 20:15 < kanzure> or just 'voltage' 20:15 < kanzure> "voltage dependent ion gates" 20:16 < fenn> the neuron stuff? dont they work on rate of change anyway? 20:17 < kanzure> http://en.wikipedia.org/wiki/Voltage-gated_ion_channel 20:17 < kanzure> ah, "voltage drop" 20:17 < kanzure> "it is possible to surmise that when a potential difference is introduced over the membrane, the associated electromagnetic field induces a conformational change in the potassium channel." 20:18 < kanzure> "The conformational change distorts the shape of the channel proteins sufficiently such that the cavity, or channel, opens to admit ion influx or efflux to occur across the membrane, down its electrochemical gradient. This subsequently generates an electrical current sufficient to depolarise the cell membrane." 20:18 < fenn> sorry kanzure but your massive page of transcribed professor speak doesn't really help 20:18 < kanzure> the wikipedia article is better :) 20:18 < fenn> as usual 20:18 < kanzure> The voltage-sensitive protein domain of these channels (the "voltage sensor") generally contains a region composed of S3b and S4 helices, known as the "paddle" due to its shape, which appears to be a conserved sequence, interchangeable across a wide variety of cells and species. Genetic engineering of the paddle region from a species of volcano-dwelling archaebacteria into rat brain potassium channels results in a fully functional 20:18 < kanzure> huh 20:18 < kanzure> there's a domain that is voltage-sensitive. how convenient. 20:19 < fenn> are you sure it's voltage sensitive or actually just ion flow sensitive? 20:19 < fenn> because without a membrane you have no ion flow 20:19 < kanzure> this article is lacking references 20:19 < kanzure> someone deserves to die 20:20 < fenn> meh 20:21 < fenn> i can see how that would work 20:21 < fenn> it could be like a snap action switch, or like a pressure regulator 20:22 < fenn> anyway if my thinking is right it's based on osmotic pressure 20:22 < fenn> or whatever the voltage-iduced equivalet is 20:22 < fenn> great now my n key is doig it too 20:23 < fenn> h seems to be better, maybe the thig just moved 20:23 * fenn shakes laptop upside dow vigorously 20:23 < genehacker_> hmmm... 20:23 < genehacker_> looks like It's going to hail here 20:24 < fenn> duck and cover 20:26 < fenn> one of these days we'll have a super duper protein simulation environment and we can just load the pdb and watch it go 20:26 < fenn> like nanorex 20:26 < splicer> fenn: I think you were right about the mismatch repair .. thanks. 20:27 < fenn> then i won't have to bother with whatever the protein scientists are calling it, i can just see how the damn thing works 20:28 -!- splicer is now known as splicer_zzz 20:28 < fenn> splicer_zzz: what does sthf stand for? 20:28 < genehacker_> sigh 20:28 < fenn> swedish transhumanist front? 20:29 * kanzure goes off to look for some food 20:29 < genehacker_> If we only had ridiculous quantum computers 20:30 < fenn> if only google would stop substituing every fucking letter in my search query 21:04 -!- xp_prg [n=xp_prg3@99.2.31.217] has quit ["This computer has gone to sleep"] 21:08 < kanzure> *** - NTH: (1) is not a non-negative integer 21:08 < kanzure> roar! 21:26 -!- mindspillage [n=kat@wikimedia/KatWalsh/x-0001] has joined #hplusroadmap 21:27 < kanzure> car of (1) is apparently a non-negative integer, however. 21:27 < kanzure> gee, that only took 20 minutes! at this rate, 21:27 * kanzure counts 21:27 < kanzure> it will only take forever. 21:34 -!- xp_prg [n=xp_prg3@98.234.52.78] has joined #hplusroadmap 21:45 < fenn> (forever) is not a non-negative integer 21:53 -!- nsh [i=sbp@59.176.232.72.static.reverse.ltdomains.com] has joined #hplusroadmap 21:58 < kanzure> #:TEETH_GOAL is not a string 21:58 < kanzure> hrm. 22:07 < drazak_> ugh 22:08 < drazak_> tomorrow I need to find papers to support my idea 22:09 -!- any62500538 [n=someone@75-121-63-19.dyn.centurytel.net] has joined #hplusroadmap 22:09 < kanzure> drazak_: have you considered doing it the other way around? 22:10 < drazak_> no :P 22:10 < drazak_> I already emailed the ide 22:10 < drazak_> atomorrow I need to email a bibliography to support it 22:10 < kanzure> maybe you should download the microfluidics paper archive in the mean time 22:11 < drazak_> got a link that I can wget? 22:12 * drazak_ going to bed 22:12 < drazak_> couple more trivia questions 22:14 < kanzure> http://heybryan.org/books/papers/microfluidics/ 22:14 < kanzure> there should be a zip file in there 22:14 < kanzure> or in the parent directory with an obvious name 22:24 -!- genehacker_ [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has quit ["ChatZilla 0.9.84 [Firefox 3.0.10/2009042316]"] 22:27 -!- katsmeow-afk [n=someone@75-120-45-44.dyn.centurytel.net] has quit [Read error: 110 (Connection timed out)] 22:27 < kanzure> "high voltage awareness day" 22:28 < kanzure> sounds like a job for 4hv.org 22:37 -!- genehacker [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has joined #hplusroadmap 22:43 < kanzure> *** - APPLY: argument (LAMBDA (A) (COND (A (LIST '/ A 'TEETH_GOAL)))) is not a function. 22:43 < kanzure> oh, I guess I should take the car of that 22:44 < kanzure> blah 22:48 < fenn> kanzure: you know.. maybe you should do some basic lisp exercises first, instead of pretending you know the language 22:49 < kanzure> if you stare at the code long enough, you somehow know it. 22:50 < fenn> or think you know it 22:50 < fenn> anyway.. what does sata want to do at the fab? 22:50 < kanzure> store stuff 22:50 < kanzure> and maybe do some wetlab stuff 22:51 < fenn> store as in hands-off or we-can-use-it 22:51 < kanzure> you know what, I'm not sure 22:51 < kanzure> he would probably be ok with sharing tools, but I doubt he'd be ok with sharing materials 22:52 -!- dizt [n=duzt@dsl093-216-054.aus1.dsl.speakeasy.net] has quit [Read error: 104 (Connection reset by peer)] 22:52 < fenn> storing materials? like chemicals? 22:52 < kanzure> I'm talking out of my ass 22:52 < kanzure> I forget what he was thinking of doing 22:53 < fenn> ok. i have no idea what sata does or wants to do 22:53 < kanzure> it's also kind of strange because he already has more than enough lab space 22:53 < fenn> right 22:53 < kanzure> one of the labs is a typical bio lab and classes go on in there 22:53 < kanzure> the other lab is a concrete attachment to the greenhouse on the sixth floor of another building 22:53 < kanzure> the greenhouse houses a few rooms, one of the rooms has our 400 gallon algae tanks 22:53 < kanzure> s/tanks/ponds/ 22:55 < kanzure> we also have some field acerage at brakenridge field labs 22:55 < kanzure> maybe he's interested in using it for development of some equipment. that would make sense. 22:55 < fenn> what does that mean exactly? 22:56 < kanzure> that means we struck ground a week ago with some machinery to dig up space for some ponds 22:56 < kanzure> 20x20x3 or so 22:56 < fenn> aha 22:56 < fenn> i want to do some aerostat/kite stuff in the indefinite future 22:56 < kanzure> random? 22:57 < fenn> for bouncing wifi or APRS around 22:57 < genehacker> heh class 22:57 < genehacker> es 22:57 < genehacker> I learned that the hard way 22:57 < kanzure> no, I mean, did we just change subjects somehow? 22:57 < kanzure> by seeing them in there? 22:57 < genehacker> there's also park that'd be great for aerostats 22:57 < genehacker> yeah... 22:57 < kanzure> what's so bad about that 22:58 < kanzure> ? 22:58 < genehacker> we got machinery? 22:58 < fenn> er.. all i meant was it'd be nice to have some open area to mess with airplanes and kites and not have some park ranger try to shoot me 22:58 < genehacker> graders or bulldozers? 22:58 < kanzure> not sure 22:58 < kanzure> I think we rented something 22:58 < kanzure> or, BFL did 22:58 < genehacker> no park ranger's gonna shoot you 22:58 < genehacker> you have to worry about dogs though 22:58 < genehacker> ] 22:58 < genehacker> it's a dog park 22:58 < fenn> well they might mess with my balloon 22:58 < genehacker> kites already fly there 22:59 < genehacker> nah dogs aren't too attracted to balloons 22:59 < fenn> heh 22:59 < fenn> where's this dog park? 22:59 < kanzure> do you mean zilker? 22:59 < genehacker> yeah 22:59 < genehacker> I've seen people kite buggy around places like that 23:00 < genehacker> I've seen kites in the trees there 23:01 < kanzure> I've seen people in the trees there 23:01 < fenn> people in the kites in the trees there? 23:02 < kanzure> thought it was some sort of failed college experiment 23:03 < fenn> is there some kind of austin wiki? 23:03 < kanzure> not that I know of. 23:05 < fenn> Connection to host www.urbanaustin.org is broken. 23:05 < fenn> oh well 23:06 < fenn> looks like it was up for a whole 4 months in 2007 23:06 < fenn> The goal of the Urban Austin project is to give the residents of Austin a complete, continually-updated online reference source of what's being planned and built. It will also serve as an online archive of this period of drastic change in the urban landscape, capturing images of a city in transition. 23:07 < kanzure> there are some commercial thingies out there, but take it with some salt 23:07 < kanzure> like austin360 23:07 < fenn> austin360 blows goats 23:08 < kanzure> yep 23:12 < genehacker> what is austin360? 23:12 < genehacker> explain why it blows goats 23:13 < kanzure> it's a terrible website 23:13 < genehacker> oh yeah 23:13 < genehacker> that... 23:14 < fenn> it's mostly ads for TV shows, and the rest is just journalists bitching 23:14 < genehacker> of course... 23:27 -!- any62500538 is now known as katsmeow 23:34 -!- xp_prg [n=xp_prg3@98.234.52.78] has quit ["This computer has gone to sleep"] 23:58 -!- genehacker [n=chatzill@pool-173-57-41-223.dllstx.fios.verizon.net] has quit [Read error: 104 (Connection reset by peer)]