--- Day changed Sat Jun 05 2010 00:07 -!- marainein [~marainein@220.253-235-112.VIC.netspace.net.au] has joined #hplusroadmap 00:08 -!- Incarnation [~lorem@66.51.250.71] has quit [] 00:43 -!- marainein [~marainein@220.253-235-112.VIC.netspace.net.au] has quit [Read error: Connection reset by peer] 01:56 -!- jrayhawk [~jrayhawk@orchitis.omgwallhack.org] has quit [Quit: leaving] 01:57 -!- jrayhawk [~jrayhawk@orchitis.omgwallhack.org] has joined #hplusroadmap 02:49 -!- wolfspraul [~wolfsprau@lucia.q-ag.de] has quit [Ping timeout: 265 seconds] 03:39 -!- Noahj [~noah@24.38.189.39] has quit [Ping timeout: 265 seconds] 03:41 -!- Noahj [~noah@24.38.189.39] has joined #hplusroadmap 03:49 -!- wolfspraul [~wolfsprau@lucia.q-ag.de] has joined #hplusroadmap 04:36 -!- marainein [~marainein@220.253-202-18.VIC.netspace.net.au] has joined #hplusroadmap 05:20 < fenn> interesting idea, pepakura repstrap http://forums.reprap.org/read.php?152,46623 05:24 -!- strages [~strages@c-76-29-243-225.hsd1.al.comcast.net] has quit [Read error: Connection reset by peer] 05:41 -!- Noahj [~noah@24.38.189.39] has quit [Ping timeout: 240 seconds] 06:52 -!- marainein [~marainein@220.253-202-18.VIC.netspace.net.au] has quit [Quit: Ex-Chat] 07:07 -!- wolfspraul [~wolfsprau@lucia.q-ag.de] has quit [Quit: leaving] 08:06 -!- randallagordon [~randallag@c-76-115-121-209.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 08:15 -!- randallagordon [~randallag@c-76-115-121-209.hsd1.or.comcast.net] has joined #hplusroadmap 08:53 < kanzure> fenn: do you read the reprap forums frequently? 09:21 < fenn> never 09:22 < fenn> wiggle stereoscopy: http://www.stereomaker.net/sample/ani/ani_e.htm 09:22 < fenn> if it doesn't make sense, you are probably a robot 09:36 -!- strages [~strages@c-76-29-243-225.hsd1.al.comcast.net] has joined #hplusroadmap 09:41 -!- CIA-46 [cia@208.69.182.149] has quit [Ping timeout: 260 seconds] 09:45 -!- Alystair [Alystair@bas1-toronto10-1279555684.dsl.bell.ca] has joined #hplusroadmap 09:53 < kanzure> http://openpcr.org/2010/06/why-we-built-openpcr/ 09:55 < kanzure> i find it weird that tito is still trying to sell $200 pieces of acrylic 09:55 < kanzure> apparently less than 10 people have purchased his gel box 09:55 < kanzure> (no surprise there) 09:58 < kanzure> "corporate anthropology" 09:58 < kanzure> i've been looking over http://crisiscommons.org/ and http://oilreporter.org/ 10:21 -!- Roy78 [~Tiger@ip68-11-187-208.br.br.cox.net] has joined #hplusroadmap 10:21 -!- Yocttar [~546f1f13@gateway/web/freenode/x-zinrlrdsuyhzdrzq] has joined #hplusroadmap 10:27 < kanzure> hi Roy78 10:27 < kanzure> hi Yocttar 10:28 -!- CIA-46 [cia@208.69.182.149] has joined #hplusroadmap 10:44 -!- ybit [~quassel@unaffiliated/ybit] has quit [Ping timeout: 252 seconds] 10:45 < Yocttar> Heya =) 10:46 < Yocttar> whats better nerd or geek? :D 10:46 < splicer> nerd... I don't understand the preoccupation with the fucking gelboxes 10:48 < Yocttar> what do you mean? which preoccupation? 10:50 < Yocttar> btw, realy thanks alot kanzure for referencing me over here, was looking for something of this "prison break" core idea kind :) 10:54 < Alystair> gelbox? 10:55 < splicer> it's the forever discussion on diybio 10:56 * Alystair should really join the mailing list 10:58 -!- lepton [~lepton@174-29-2-161.hlrn.qwest.net] has joined #hplusroadmap 11:00 < kanzure> Alystair: http://groups.google.com/group/diybio 11:18 < kanzure> re: biocurious: http://huff.to/crJuUh 11:18 < kanzure> which redirects to http://www.huffingtonpost.com/wires/2010/05/27/biocurious-the-bay-area-b_ws_592862.html 11:18 < kanzure> which is just a front for http://fora.tv/2010/05/23/BioCurious_The_Bay_Area_Biology_Collaborative_Lab_Space 11:18 < kanzure> gah i wish links didn't suck so much 11:30 -!- ybit [~quassel@unaffiliated/ybit] has joined #hplusroadmap 11:35 < Alystair> beat you to it 11:54 -!- genehacker [~notanemai@cpe-66-68-104-134.austin.res.rr.com] has joined #hplusroadmap 12:01 -!- ybit [~quassel@unaffiliated/ybit] has quit [Ping timeout: 240 seconds] 12:20 < splicer> (looking at Titos mullet I wonder who the first with a mohawk will be) 12:35 -!- InkBlob [~fsdffasdf@S01060016b6ee2b72.vf.shawcable.net] has joined #hplusroadmap 13:22 -!- parolang [~user@8e4a01246100775874c4f448e9887093.oregonrd-wifi-1261.amplex.net] has joined #hplusroadmap 13:54 -!- Roy78 [~Tiger@ip68-11-187-208.br.br.cox.net] has quit [Ping timeout: 240 seconds] 14:02 -!- Noahj [~noah@pool-96-237-189-146.bstnma.fios.verizon.net] has joined #hplusroadmap 14:51 -!- lepton [~lepton@174-29-2-161.hlrn.qwest.net] has quit [Read error: Connection reset by peer] 15:24 -!- ybit [~quassel@unaffiliated/ybit] has joined #hplusroadmap 15:26 -!- phryk [~phryk@yggdrasil.phryk.net] has joined #hplusroadmap 15:26 < kanzure> hello phyrk 15:26 < phryk> Hi there 15:26 < kanzure> what's up? 15:26 < phryk> Not much ybit told me to join ;P 15:27 < phryk> What exactly is hplusroadmap? 15:27 < ybit> !log 15:27 < ybit> hrm :) 15:28 < kanzure> hplusroadmap: You will never find a more wretched hive of scum and villainy on the internet. You must be cautious. 15:28 < ybit> http://designfiles.org/irclogs.txt 15:29 < kanzure> this is the one that is kept updated: http://gnusha.org/irclogs.txt 15:29 < ybit> gracias 15:30 < phryk> kanzure: That kinda sounds like /b/... 15:33 < kanzure> http://letsfreckle.com/ sounds an awful lot like http://rescuetime.com/ 15:33 < nsh> unicode ellipses look really stupid in fixed-width fonts 15:34 < kanzure> phryk: we focus on transhuman tech, singularities or accelerating trends, and uh 15:34 < kanzure> something else 15:36 < nsh> comfy chairs 15:36 < phryk> Ah nice 15:36 < phryk> Like Aubrey deGrey or whatever his name was? 15:37 < nsh> he's actually our telepresence hairbot 15:37 < kanzure> "Love the streamed sets because they don't interrupt your workflow. It's like being at a coding dance party; for hours." 15:37 < phryk> Okay^^ 15:38 < kanzure> http://trancearoundtheworld.com/ 15:38 < phryk> coding dance party sounds like some real fun 15:39 < phryk> Mh, not really my type of music 15:39 < phryk> Only stream i (seldomly) listen to is ... dead as i just had to witness now :/ 15:40 < phryk> Poor uppercut radio :'( 15:42 < genehacker> anyone here buy stuff from mcmaster-carr? 15:43 < genehacker> oh shoot where's the streams? 15:47 < kanzure> genehacker: here's some different streams: http://designfiles.org/~bryan/music/url.txt 15:47 < kanzure> and stuff i've found that i like is listed here: http://designfiles.org/~bryan/music/ 15:48 < kanzure> right now i'm on http://www.di.fm/mp3/vocaltrance.pls (doing some facebook app dev bullshit) 15:49 < genehacker> oh I thought you were at hplus 15:49 < kanzure> wait did i miss my flight? 15:49 < kanzure> i thought the event started on the 12th 15:49 < genehacker> I am having temporal awareness problems 15:50 < genehacker> anyway can I visit the space tomorrow 15:50 < kanzure> sure, do you need a ride? 15:51 < genehacker> yeah 15:51 < kanzure> alright 16:11 < phryk> You guys have a dedicated hackerspace? 16:12 < genehacker> #austinhackerspace for more info 16:13 < phryk> That's a creative name :P 16:15 < phryk> Ooh 16:15 < phryk> splicer: We meet again :D 16:16 < phryk> All that stuff around transhumanism, diybio and biopunk seems to be pretty small currently^^ 16:20 < kanzure> small? 16:21 < phryk> Yes. 16:21 < phryk> You keep running into the same people over different routes^^ 16:22 < phryk> I meant the community 17:48 < Alystair> not many people realize change is necessary ;) 17:49 < phryk> Well for life, change is always necessary. 17:50 < phryk> Stagnation practically is death... 17:50 < phryk> Also, I'm going to sleep now ;P 17:50 < phryk> Good night ;) 18:09 -!- Phreedom [~quassel@109.254.6.63] has quit [Ping timeout: 240 seconds] 18:16 -!- genehacker [~notanemai@cpe-66-68-104-134.austin.res.rr.com] has quit [Ping timeout: 258 seconds] 18:18 -!- wolfspraul [~wolfsprau@lucia.q-ag.de] has joined #hplusroadmap 18:29 -!- Roy78 [~Tiger@ip68-11-187-208.br.br.cox.net] has joined #hplusroadmap 18:44 -!- ybit [~quassel@unaffiliated/ybit] has quit [Remote host closed the connection] 18:44 -!- ybit [~quassel@unaffiliated/ybit] has joined #hplusroadmap 18:58 -!- lepton [~lepton@174-29-2-161.hlrn.qwest.net] has joined #hplusroadmap 19:06 < kanzure> /window 213 19:06 < kanzure> crap 19:06 < kanzure> second time today :( 19:20 -!- Incarnation [~lorem@66.51.250.71] has joined #hplusroadmap 19:20 -!- Incarnation [~lorem@66.51.250.71] has quit [Client Quit] 19:28 -!- genehacker [~notanemai@cpe-66-68-104-134.austin.res.rr.com] has joined #hplusroadmap 19:28 < genehacker> http://www.mother-of-robots.org/ 19:44 -!- Roy78 [~Tiger@ip68-11-187-208.br.br.cox.net] has quit [Ping timeout: 252 seconds] 19:51 < jrayhawk> do you seriously have 213 windows open 19:52 < jrayhawk> that made steve laugh 19:53 * nsh wouldn't be at all surprised 19:54 < kanzure> jrayhawk: do you even know who i am? 19:54 < kanzure> jrayhawk: http://heybryan.org/shots/2008-01-15-tabs.png is common 19:55 < nsh> reminds me, have you done a bookmark dump lately? 19:55 < kanzure> my bookmark managers all crashed and refuse to work with me 19:55 < kanzure> so i don't use them any more 19:55 < kanzure> back to memorizing urls! 19:56 < nsh> ah 19:56 < nsh> let me know when you do a brain dump then :) 20:00 * kanzure fiddles around with http://jambool.com/ for "virtual currency" 20:11 < nsh> there any other kind? 20:11 < kanzure> what? 20:11 < nsh> any other kind of currency than virtual 20:11 < nsh> fiat 20:11 < kanzure> no 20:12 < genehacker> sounds like you're making one of those facebook crack addiction simulations things... 20:12 < egeste> hello 20:13 < kanzure> genehacker: yep, that's exactly what i'm doing 20:13 < kanzure> hi egeste 20:13 < genehacker> with other people? 20:13 < kanzure> ? 20:13 < kanzure> what? 20:13 < nsh> was reading about lycurgus of sparta, who instituted a currency made of iron coins, dipped in vinegar to become brittle and intrinsically worthless 20:13 < genehacker> you said you were having a programming party earlier 20:13 < nsh> http://www.e-classics.com/lycurgus.htm 20:14 < nsh> to eliminate the desire to acrue wealth 20:14 < genehacker> well that would eliminate the inflation problem 20:14 < genehacker> unfortunately that would eliminate inflation 20:14 * nsh smiles 20:15 < nsh> yes lisa, crisotunity! 20:15 < kanzure> genehacker: no that was a quote that i copied-and-pasted 20:15 < kanzure> i'm doing the facebook app on my lonesome 20:15 < kanzure> just a learning experience 20:15 < kanzure> it will be demoed at h+ summit 2010 by andrew hessel,l though 20:15 < kanzure> presumably exxon is going to dump a few million into it.. but whatever 20:16 < kanzure> i don't expect that to actually happen 20:16 < genehacker> so it's something more useful than facecrack? 20:16 < nsh> app does what? 20:16 * nsh is considering writing a flashcard (recall aid) app for facebook 20:17 < nsh> or, more ambitiously, a collaborative rune goldberg machine construction game 20:17 < kanzure> it's a virtual currency on top of http://oilreporter.org/ and http://crisiscommons.org/ - an auction site for co2 fixation-related activities 20:18 < kanzure> nsh: fantastic contraption? :) or skdb? :D 20:18 < nsh> simulated :) 20:18 < kanzure> actually my 80/20 company might be interested in an app for collaborative 80/20 design bullshit 20:18 < kanzure> on facebook 20:18 < genehacker> so you can own virtual money that's as good as real money that you don't have to pay taxes on? 20:18 < kanzure> as a way to market it to engineers to get kiddos interested in actually building shit 20:18 < kanzure> genehacker: yep 20:18 < nsh> 80/20 company? 20:19 < kanzure> canadian oil companies have to pay $15/ton of CO2 in taxes 20:19 < genehacker> a cad application for facebook might be cool 20:19 < kanzure> nsh: structory.com-- basically it's a shopping cart frontend to skdb specifically for 80/20 modular beam components 20:19 < kanzure> genehacker: or totally lame 20:19 < nsh> oh 20:19 < genehacker> well it would be less lame than farmville 20:20 < genehacker> DESIGN A PNEUMATIC PROPORTIONAL VALVE AND GET 50 CAD COINS... 20:20 < genehacker> or something like that 20:21 < nsh> invite your friends to debug your Skynet defence system 20:21 < genehacker> and use classic facebook app user enslavement practices, and soon you've crowd sourced design 20:21 < genehacker> exactly 20:22 * nsh needs to read some good studies of wikipedian social development 20:22 < nsh> there are critical developmental phases 20:23 -!- parolang [~user@8e4a01246100775874c4f448e9887093.oregonrd-wifi-1261.amplex.net] has quit [Ping timeout: 258 seconds] 20:23 < nsh> steerage is all the more important for mechanical tower of babel 20:24 < jrayhawk> god your server is so slow 20:24 < jrayhawk> why aren't you using barthosting for that 20:24 < kanzure> jrayhawk: i should movei t somewhere 20:24 < kanzure> i have about 2 TB of bullshit on the server 20:24 < kanzure> can i just.. ship it to you? 20:24 < genehacker> time warner cable slow speed? 20:24 < jrayhawk> oh i might have to convince bart to do some upgrades, then. I think we only have a terabyte on there. 20:25 < kanzure> i can be convinced to only do a few hundred meg 20:25 < kanzure> ok convinced 20:25 < jrayhawk> I guess if you're willing to let me keep the drives, I can just shove 'em in the server. 20:25 < kanzure> my upload is terrible, that's why i've never moved it anywhere 20:25 < nsh> clone the disks 20:25 < kanzure> yeah i should. 20:25 < kanzure> blah 20:26 < kanzure> jrayhawk: can i use the gnusha.org virtual server for this 20:26 < genehacker> kanzure you wouldn't happen to know if the university has faster internet than you do? 20:26 < kanzure> the university definitely has faster internet than i have 20:26 < jrayhawk> Yeah, sure. Just let me know ahead of time if you seriously intend to fill up the 600GB we have available to you. 20:26 < genehacker> really? 20:26 < kanzure> jrayhawk: k 20:26 < kanzure> genehacker: yeah.. check out the speeds on designfiles.org 20:27 < jrayhawk> And yes, you can send me drives for me to deal with. 20:27 < genehacker> do you have wood and drills in the hacker space then? 20:27 < kanzure> lots of wood 20:27 < kanzure> lots and lots of wood 20:27 < kanzure> also lots of dust 20:27 < kanzure> but that's unrelated :) 20:27 < genehacker> I'm making an antenna mount then 20:27 < genehacker> dust is fine too 20:28 < genehacker> especially sawdust, we could try making a boat out of pykrete... 20:30 < jrayhawk> Kanzure: you've seen newsbeauter, right? 20:30 < jrayhawk> err newsbeuter 20:31 < kanzure> no. /me checks 20:32 < jrayhawk> It is the mutt of RSS readers. 20:32 < kanzure> hm 20:32 < kanzure> i need an automatic rss download script thingy so that i don't have to actually use twitter 20:32 < kanzure> i.e. because they delete their contents after 24 hours or something 20:32 < jrayhawk> More optimally you should be using rss2email along with sup or notmuch 20:35 < kanzure> my inbox is a bullshit free zone 20:35 < kanzure> well, actually.. 20:38 < jrayhawk> Well, that's why you use a sieve system to classify your mail into folders (if imap) or tags (if local storage) 20:39 < jrayhawk> I'm a little alarmed you don't do that already. 20:39 < kanzure> what? 20:40 < kanzure> i was making a witty comment 20:40 < jrayhawk> oh okay 20:40 < kanzure> i use tags and folders 20:40 < jrayhawk> tank goodness 20:40 < kanzure> my inbox is still 68,862 unread messages however 20:40 < jrayhawk> golly 20:41 < kanzure> however, according to the gmail.com interface my entire mail collectio nis 210,847 20:41 < kanzure> *collection is 20:41 < InkBlob> 68,000 unread? do you have a fan mailing list or something? 20:41 < kanzure> (also, i'm lagging today and am having rtouble typing) 20:41 < kanzure> considering 68k unread, and 210k total, i'd say that's pretty good 20:42 < InkBlob> i read every message sent to me. wuw kanzure. 20:42 < kanzure> jrayhawk: have you seen this? http://designfiles.org/~bryan/meetlog/meetlog.txt 20:42 < kanzure> InkBlob: so do i. a lot of it is not personally addressed to me 20:43 < jrayhawk> yes, and i am very pleased about your religious documentation of having talked to BUTT_PORBLEMS 20:43 < InkBlob> ow 20:43 < kanzure> jrayhawk: you're welcome 20:43 < kanzure> InkBlob: you're also welcome to send me fan mail 20:43 < kanzure> you know, if you feel like it 20:44 * nsh offers to send a single peanut in an envelope 20:44 < kanzure> ? 20:44 < kanzure> are you calling me a nut 20:44 < kanzure> :P 20:44 < nsh> no, i just have a bunch of peanuts :) 20:44 < kanzure> then by all means 20:44 < nsh> more seriously though 20:44 < nsh> if you had a million SD cards 20:44 < nsh> say 2 gig 20:45 < nsh> and you could send them to a million people who would look at their contents 20:45 < nsh> what would you send? 20:45 < nsh> i might ask this on reddit or something 20:45 < genehacker> you should 20:45 < jrayhawk> goatse 20:45 < kanzure> a trojan? 20:45 < nsh> i wonder what a million SD cards costs at price 20:45 < kanzure> probably peanuts 20:45 < kanzure> speaking of which.. 20:46 * nsh chews 20:46 < jrayhawk> do not chew on goatse that is gross you are gross 20:47 < nsh> hey, it's palliative chewing 20:47 < kanzure> i like how our first ideas were goatse and a trojan 20:49 < nsh> revealing 20:49 < nsh> damn, that last mouthful of peanuts tasted amazing 20:56 -!- GreydonSquare [~Zartek@ip68-2-35-220.ph.ph.cox.net] has joined #hplusroadmap 20:56 -!- jumpboy11j [~jumpboy11@pool-98-109-133-51.nwrknj.fios.verizon.net] has joined #hplusroadmap 20:57 < kanzure> hi GreydonSquare 20:57 < kanzure> hi jumpboy11j 20:58 < jumpboy11j> hi 20:58 < jumpboy11j> any discussion going on here? 20:58 * jumpboy11j doesnt want to interrupt any discussion that may be going on 20:58 < kanzure> only about world domination and cybernetics 20:58 < kanzure> what's up? 20:58 < GreydonSquare> Hey Kanzure, sorry i was over in #newmars 20:59 < jumpboy11j> eh not much 20:59 < GreydonSquare> i love both topics 20:59 < jumpboy11j> you? 20:59 < GreydonSquare> lol 20:59 < kanzure> ah, the mars society 20:59 < jumpboy11j> yeah 20:59 < GreydonSquare> yup 20:59 < jumpboy11j> although more New Mars than Mars Society 20:59 < jumpboy11j> even though NM is TMS forum 20:59 < kanzure> did you ever see openvirgle? 20:59 < kanzure> an open source go at the virgle project? 20:59 < jumpboy11j> no 21:00 < kanzure> http://openvirgle.net/ 21:00 < kanzure> kinda related to http://openmanufacturing.org/ 21:03 < kanzure> http://www.emcore.com/assets/photovoltaics/Paper_Navid_9-22-00.pdf 21:08 < kanzure> beep bloop 21:08 < jumpboy11j> doesn't look like we're talking about money anymore 21:09 < kanzure> fenn: how goes the goo squirter? 21:09 < jumpboy11j> how does resource allocation in the scientific sense sound? 21:09 < jumpboy11j> IE production and distribution but not money 21:10 < kanzure> you know, i really have too many irc channels open for me to split off conversations into yet another channel :) 21:10 < jumpboy11j> haha 21:11 < jumpboy11j> okay 21:11 < kanzure> but josh is welcome to come back in here :P 21:11 < jumpboy11j> haha 21:11 < jumpboy11j> so you're a transhumanist? 21:11 -!- GreydonSquare [~Zartek@ip68-2-35-220.ph.ph.cox.net] has quit [Quit: Leaving] 21:11 < kanzure> yeah, sure 21:12 -!- jasonwohlfahrt [~chatzilla@cpe-67-11-208-33.satx.res.rr.com] has joined #hplusroadmap 21:12 < jumpboy11j> I might or might not call myself one 21:12 < kanzure> hi jasonwohlfahrt 21:12 < jumpboy11j> except I'm not exactly sure what transhumanism is 21:12 < kanzure> basically it's the idea of augmenting, upgrading, and getting rid of your body (this is the minimal definition i guess) 21:12 < kanzure> well, not getting rid necessarily 21:13 < jumpboy11j> might be amalgated into a larger whole 21:13 < kanzure> primarily it's a movement of people who like to thin kabout the future and don't do much 21:13 < jumpboy11j> but that's inevitable with the progression of progress isnt it? 21:13 < genehacker> not necessarily 21:13 < jumpboy11j> how so? 21:14 < jumpboy11j> if technology keeps advancing, that is the clear next step 21:14 < kanzure> there's this concept of "recursive self-improvement" floating around, but nobody has nailed down a usable quantification of it 21:14 < genehacker> what if we develop nuclear bombs that anyone can make in their basement? 21:15 < jumpboy11j> well that would be antithetical to progress if they were used 21:15 < jumpboy11j> that's an existential threat 21:15 < genehacker> what scares me is someone releasing smallpox 21:15 < genehacker> north korea might have smallpox stocks 21:16 < jumpboy11j> anyone might 21:16 < lepton> All good reasons to spread civilization(s) beyond the earth 21:16 < jumpboy11j> hell I'm sure the US does 21:16 < jumpboy11j> yeah 21:16 < kanzure> lepton: nobody understands that :( 21:16 < genehacker> the US does 21:16 < lepton> I'm excited for SpaceX this week 21:16 < jumpboy11j> they launched already 21:16 < lepton> I've got a friend working with them 21:16 < genehacker> me too 21:16 < lepton> Successfully got into orbit 21:16 < kanzure> lepto i'd be interested in figuring out a way to convince people that we need to engineer reliability into our civilization 21:16 < genehacker> we really do need to expand beyond earth 21:16 < jumpboy11j> definitely 21:17 < genehacker> first define reliability 21:17 < genehacker> certainly you don't mean a factor we use when figuring out how long a part will last 21:17 < kanzure> there's a lot of quantifications of reliability 21:17 < kanzure> look into reliability theory 21:17 < kanzure> hell even the basic saying "don't put all of your eggs in one basket" 21:18 < lepton> Recently I've been trying to appreal the techo-utopian idealism of the 50's that a lot of people have in mind 21:18 < genehacker> that's redundancy 21:18 < lepton> reminding them that finding the prospect of space travel as silly is a recent social developtment 21:18 < kanzure> yes redundancy is one part of reliability 21:18 < jumpboy11j> also simplicity 21:18 < kanzure> yep 21:18 < jumpboy11j> but my question is still 21:19 < genehacker> somethings cannot be simplified 21:19 < kanzure> there are four main points as i see it: redundancy, simplicity, transparency, and uh 21:19 < kanzure> crap why do i always forget the other one? 21:19 < kanzure> http://en.wikipedia.org/wiki/Single_point_of_failure 21:19 < kanzure> diversity :) 21:19 < jumpboy11j> aren't simplicity and diversity antithetical to some extent? 21:20 < kanzure> well, there's simplicity in certain forms of emergence 21:20 < genehacker> heh where's that ghost in the shell quote about diversity... 21:20 < kanzure> whereas under neath that layer of emergence (or "emergent order") there's actually really complex things happening 21:20 < lepton> A fractal is a good example of both simplicity and diversity 21:21 < lepton> a mandelbrot of such 21:21 -!- jasonwohlfahrt [~chatzilla@cpe-67-11-208-33.satx.res.rr.com] has quit [Ping timeout: 240 seconds] 21:21 < genehacker> "If we all reacted the same way, we'd be predictable, and there's always more than one way to view a situation. What's true for the group is also true for the individual. It's simple: overspecialize, and you breed in weakness. It's slow death." 21:21 < genehacker> ah that one 21:22 < jumpboy11j> still 21:22 < kanzure> lepton: do you know about the seasteading insitute? 21:22 < kanzure> *institute 21:22 < jumpboy11j> how is transition different from any other form of futurism or progress 21:22 < jumpboy11j> or even consumerism? 21:22 < kanzure> what? 21:22 < kanzure> "how is transition" ? what is "transition"? 21:23 < jumpboy11j> sorry 21:23 < jumpboy11j> its late 21:23 < jumpboy11j> transhumanism 21:23 < genehacker> this is sounding like philosophy 21:23 < kanzure> EROR ABORT 21:23 < jumpboy11j> I just want to get a handle on what transhumanism is 21:23 < kanzure> ERROR 21:23 < kanzure> god damn it 21:23 < kanzure> jumpboy11j: http://heybryan.org/transhumanism_def.html read the part in green at the top maybe :)" 21:23 < kanzure> without the quote mark blah 21:24 < jumpboy11j> thanks 21:24 < lepton> Yeah, I know of seasteading 21:24 < lepton> I like the idea 21:24 < InkBlob> discuss most humanly possible way to extend your lifespan with today's tehnology. 21:24 < lepton> I like it most in the asteroid belt 21:24 < InkBlob> cell printing? 21:25 < jumpboy11j> does uploading onto a computer count? 21:25 < kanzure> InkBlob: did you read the papers yet? 21:25 < InkBlob> i didn't know it existed. 21:25 < jumpboy11j> well not yet 21:25 < kanzure> lepton: i think that petri friedman's idea is nice and all.. i like the idea of letting a thousand flowers bloom for different ways of doing civilization 21:25 < kanzure> and having a "civilization seed" lets that happen :) 21:25 < kanzure> lepton: more on that is here: http://groups.google.com/group/openmanufacturing/msg/e4c375acce772250 21:26 < kanzure> jumpboy11j: btw, the papers that i just mentioned to InkBlob are over here: http://designfiles.org/papers/longevity/ 21:26 < InkBlob> no kanzure not yet, but i will definetly go knee deep into it. right now, i'm trying to make cash. 21:27 < InkBlob> yup that link is in my list. 21:27 < kanzure> InkBlob: if i were you, i would focus on the blood-based stem cell therapies for longevity research 21:27 < nsh> don't try and make cash, for that is impossible, only try to realise the truth, that there is no cash 21:27 < kanzure> i.e. blood-based therapies i mean 21:27 < InkBlob> yes, stem cell therapy. i thought that was the most plausible topic. 21:27 < kanzure> nsh: :) 21:27 < InkBlob> blood based. got it. 21:28 < kanzure> nsh: actually i've kind of always imagined you looking like that bald kid 21:28 < InkBlob> is there anyother kind though? 21:28 * nsh smiles 21:28 * kanzure steals nsh's spoon 21:29 < nsh> aerosol stem-cell therapy would be interesting 21:29 < kanzure> good thing it doesn't exist huh 21:29 < nsh> uite 21:29 < nsh> *quite 21:29 < kanzure> some jerkwads were selling oxytocin sprays a while back 21:29 < kanzure> i don't recall any studies on the efficacy though 21:29 < genehacker> well oxytocin microdarts would be more interesting 21:29 < kanzure> oxytocin ninja darts 21:29 < kanzure> gene ninjas! 21:30 < InkBlob> spraying over the vody regenerative cells i read about that. it was amazing. is it even possible? 21:30 < InkBlob> kewl 21:30 < genehacker> if you make the dart thin enough they won't ever notice 21:30 < InkBlob> v=b 21:30 < kanzure> velocity equals the drag due to wind resistence? wut? 21:30 < kanzure> oh typo 21:30 < InkBlob> no way. i got a strand of hair in my skin once and it hurt. 21:31 < kanzure> lepton: obviously i meant to say patri friedman, not petri :) 21:31 < kanzure> i was thinking of mr. petri dish i guess 21:32 < genehacker> well then have someone accidently bump into your target with an umbrella 21:32 < lepton> ha, I didn't even notice, actually 21:33 < genehacker> http://en.wikipedia.org/wiki/Georgi_Markov 21:33 < kanzure> it was eating away at my soul 21:33 < kanzure> egeste: are you at the space right now, and do you have documentation? 21:33 < splicer> a problem with stem cell therapy is that one wants to regenerate as many cells that die... how does one regulate that? 21:33 < splicer> as die 21:33 < kanzure> splicer: there are certain genetic pathways that you want to trigger and manipuolate 21:33 < kanzure> before the cells die 21:33 < kanzure> *manipulate 21:34 < splicer> yeah... if they're bot already gone 21:34 * nsh thought of petri dishes earlier when he saw the bacterial colonies growing in the wok in his (shared with 11 ablutophobic flatmates) kitchen sink 21:34 < nsh> (ablution: washing shit) 21:34 < genehacker> nsh are they glowing? 21:34 < kanzure> i'm going to paste some stuff on aging, hope nobody hates me for the flood 21:34 < kanzure> Ageing works like this: 21:34 < kanzure> 1) Electrons leak from mitochondrial Complexes I & III forming superoxide anion. 21:34 < kanzure> 2) The increasing rate of leakage and the further breakdown of mitochondrial components with time under the superoxide (...and peroxynitrite & peroxide) onslaught is strongly determined by genetically controlled factors: 21:34 < genehacker> you aren't supposed to wash woks 21:34 < nsh> genehacker, haven't checked 21:34 < kanzure> i.e., the structure of mitochondrial proteins and membranes. Hence leakage is species-specific. 21:35 < kanzure> The net effect is that leakage is a mostly monotonically increasing function of time. 21:35 < kanzure> 3) While the charged Superoxide Anion does not escape to the cytoplasm easily from the mitochondrial outer membrane... 21:35 < kanzure> 4) Mitochondrial Superoxide Dismutase (SOD) converts Superoxide Anion into Hydrogen Peroxide. 21:35 < kanzure> 5) H2O2 is neutral and easily diffuses through the mitochondrial outer membrane, entering the cytoplasm. 21:35 < kanzure> 6) In the cytoplasm, peroxide prompts protein kinase C variants (incl.epsilon) to upregulate cytoplasmic NOX. 21:35 < kanzure> NOX outputs O2-; and eventually via cytoplasmic SOD, H2O2; acting as a high-gain peroxide amplifier. 21:35 < kanzure> THIS IS AGE-LINKED, ENDOGENOUS CHRONIC OXIDATIVE STRESS. 21:35 < kanzure> 7) H2O2 is an important signalling molecule in stress signal transduction; such transduction is ramped up. 21:35 < kanzure> The MAPK/ERK cascade is overactivated. 21:35 < kanzure> The key stress transcription factors NF-kappaB; AP1 etc are constitutively upregulated. 21:35 < kanzure> Angiotensin2 is upregulated. The PI3K-Akt signalling cascade is chronically activated. 21:35 < kanzure> The transcription factors FOXO3A (and other FOXOs) are phosphorylated by Akt and translocated from the nucleus. 21:35 < kanzure> Bad is similarly phosphorylated. mTOR is 'non-phosphorylatively' activated by Akt. 21:35 < kanzure> Note that FOXOs, Bad, mTOR are downstream of PI3K. SIRT1 & klotho are upstream. 21:35 < kanzure> 8) These factors upregulate the transcription of cytokines and a whole range of stress molecules; unleash a chronic cytokine onslaught on the cell & tissues. 21:35 < kanzure> A number of runaway positive feedback loop including TLRs and RAGE is ensured. 21:35 < kanzure> 9) Under the signalling from the transcribed products, massive tissue remodelling and physiological change at the cell, tissue system & organism level is enforced. 21:35 < kanzure> Both Hypertrophic & Degenerative signalling abound. 21:35 < kanzure> 10) ...and checkpoints are disabled promoting oncogenesis;apoptosis is aberrant 21:35 < kanzure> 11) ...and stem cell niche failure - either through complete abrogation; or through enforced quiescence is the norm. 21:36 < kanzure> 12) ...and mitochondrial biogeneisis is abrogated, OXPHOS is downregulated 21:36 < kanzure> 13)...and cell senescence is upregulated, in part through p38MAPK 21:36 < genehacker> I thought there were some findings that made oxidative stress theory look bad 21:37 < genehacker> would reengineering our mitochondria help us live longer? 21:37 < kanzure> aubrey wanted to move the mitochondrial genes over into the nuclear genome, but i don't remember why 21:37 < genehacker> like replace our mitochondria with mitchondria from stuff that lives really long? 21:37 < kanzure> anyway, oxidative stress is definitely not the only reason your body dies 21:38 < genehacker> genes in the mitochondria might mutate more? 21:38 < splicer> kanzure: those patways are instant.... adding the stem cells isn't 21:38 < nsh> i ever tell you that story about Henry Ford? 21:39 < genehacker> apply mass production to what nsh? 21:39 < nsh> Henry Ford calls in his top engineers one day and says "I want you to go out to all the scrapyards in the area, and find all the ford cars, and get me a list of all the parts that have worn the least in those cars" 21:39 < nsh> the engineers go out and do this, thinking maybe he's gonna try and use these parts as examples to make the other components more durable 21:40 < nsh> when they report back to him, he simply says "make these parts to a lower specification from now on" 21:40 < kanzure> heh 21:40 < genehacker> heh 21:40 < kanzure> stop reading my brains, genehacker 21:40 < jrayhawk> heh 21:40 < genehacker> now we're to the point where we do that before hand 21:40 < nsh> i strongly suspect ageing is death by a thousand cuts, give or take. there'd be pressure to have everything start to unwind at more or less the same rate 21:41 < jrayhawk> It'd be simpler to have a handful of trigger conditions that make death succict so as to help the herd. 21:41 < genehacker> IE we understand how mechanical systems wear so people start making them to be cheaper and not last forever 21:42 < genehacker> so the saying "they don't make them like they used to is true" 21:42 < nsh> thing is, immortality has been solved already, just at a different scale level to the one we experience 21:42 < jumpboy11j> bigger or smaller? 21:42 < splicer> hela-cells? 21:42 < genehacker> immortality is highly improbable 21:43 < kanzure> "The BIG problem with traditional IV stem cell transplantation: cancer & 1st pass pulmonary filtration, is avoided with the use of these cells which are evolutionarily equipped to handle temporary niche-independence while they are mobilized in the circulation." 21:43 < nsh> we want to preserve the analogue system, when it's the digital data (genes) that have already achieved it 21:43 < kanzure> "I am currently trying to find ways of expanding harvested populations in vitro; with a view to reintroduction. This is difficult, because free stem cells tend to either differentiate or to transform. Additionally, the reintroduction has to be into a FULLY prepped organism, to simulate youth (UNDER 2 years of age) AND major trauma - which should start the various innate repair programs." 21:43 < kanzure> "During the autologous I.V. transplant I would have to inhibit SOCS3 & upregulate thymosin beta-4 and suppress systemic constitutive NF-kappaB; among other things." 21:43 < kanzure> "Use of this protocol at intervals will possibly allow maybe a 200 year lifespan; and also PAINLESS GENETIC REENGINEERING OF ADULT ORGANISMS." (ignore the caps lock) 21:43 < kanzure> http://designfiles.org/papers/longevity/2010-03-23.txt 21:44 < nsh> hmm 21:44 < splicer> some nut? 21:44 < kanzure> no :) 21:44 < kanzure> although he is nutty at times- he apologizes to me for "wasting" my time 21:44 < nsh> :) 21:45 < nsh> i wonder what he means by "painless" 21:45 < kanzure> probably "relatively"? :D 21:45 < kanzure> hm 21:45 < InkBlob> this stuff is crazy. 21:45 < InkBlob> i'm trying to wrap my head around it. 21:46 < InkBlob> i thought you were the robot-machine guy kanzure, how do you know so much about biology? 21:46 < kanzure> huh? my background is more in biology than in industrial automation 21:46 < kanzure> sadly i wish this was the other way around 21:46 < nsh> convergence :) 21:47 < genehacker> I'm more of a machine guy here 21:47 < kanzure> i just get mesmorized by this stuff: 21:47 < kanzure> 0EB93E6E02E5CF17 21:47 < kanzure> blah 21:47 < kanzure> http://www.youtube.com/user/kanzure#grid/user/0EB93E6E02E5CF17 21:47 < InkBlob> it is? how come you talk about robot arms and stuff like that in IRC all the time. 21:47 < kanzure> (my machine porn playlist) 21:48 < kanzure> because i recently bought a robotic arm :) 21:48 < InkBlob> well, great study. thangs. 21:48 < kanzure> what? 21:48 < InkBlob> Okay. 21:48 < InkBlob> great study for me. good information. thanks. 21:48 < kanzure> ah 21:48 < genehacker> kanzure I should give you my gear porn library 21:48 < kanzure> i have this song stuck in my head now: http://www.youtube.com/user/kanzure#p/c/0EB93E6E02E5CF17/37/wbDwUWGvwq4 21:49 * nsh thinks about the new Game of Life construction -- Gemini, the first oblique spaceship 21:49 < nsh> http://www.conwaylife.com/wiki/index.php?title=Gemini 21:50 < kanzure> nsh: have you seen golly? 21:50 < nsh> aye, haven't tried yet 21:50 < kanzure> http://golly.sf.net/ or something 21:50 < genehacker> a new spaceship! 21:50 < kanzure> my aussie friend has this 8 GB OSX setup running a session of golly for the past 1.5~ years 21:50 < nsh> heh 21:50 < nsh> on the same input? 21:50 < genehacker> I have this song stuck in my head: 21:50 < genehacker> http://www.youtube.com/watch?v=uJ4nmG39QXo 21:50 < kanzure> yeah just one huge continuous run 21:51 < kanzure> http://thewildca.com/ 21:51 < kanzure> he printed it out? hah 21:51 < genehacker> how do people discover these things? 21:52 < genehacker> brute force? 21:52 < nsh> nah, there's a whole 'technology' of game of life components and their interaction 21:52 < kanzure> egeste and nsh probably will tell you something about swhack or subgenius or something 21:52 < kanzure> wolfram would wave his hands and make up bullshit 21:52 < kanzure> and feynman would just smirk 21:52 < kanzure> (or something) 21:52 < nsh> hit the bongos 21:52 -!- jumpboy11j [~jumpboy11@pool-98-109-133-51.nwrknj.fios.verizon.net] has left #hplusroadmap [] 21:52 < kanzure> ah, right 21:52 < kanzure> yeah, he'd do that 21:53 < kanzure> aww jumpboy11j left :( 21:53 < genehacker> I know of the technology of game of life components 21:53 < genehacker> I'm not aware of how people discover it 21:53 < genehacker> I guess people engineer it? 21:53 < kanzure> wolfram had this classification system for CA rules 21:53 < lepton> Sounds like a good application for genetic optimization to me 21:53 < genehacker> the bullshit one that got disproven? 21:54 < nsh> i'd call it engineering at this point, genehacker. but i'm a layman 21:54 < kanzure> nsh: pfft, yeah and i'm jesus 21:54 < kanzure> wait 21:54 < genehacker> engineering as opposed to cathedral building 21:54 < genehacker> engineering is planning something out in advance 21:54 -!- lepton [~lepton@174-29-2-161.hlrn.qwest.net] has quit [Remote host closed the connection] 21:54 < nsh> see this thread: http://conwaylife.com/forums/viewtopic.php?f=2&t=399&start=0 21:54 < kanzure> gah what's with you people and quitting 21:54 < genehacker> cathedral building is making corrections as you go along 21:54 -!- Roy78 [~Tiger@ip68-11-187-208.br.br.cox.net] has joined #hplusroadmap 21:54 < nsh> the sheer level of lingo is impressive in itself 21:55 < kanzure> nsh: do you read the NKS forums too? 21:55 * nsh reads things here and there :) 21:55 < genehacker> so what's golly? 21:55 < nsh> golly is a life simulator 21:55 < nsh> uses the hashlife algorithm, for speediness 21:55 < kanzure> hi Roy78 .. you missed our longevity stem cell therapy stuff 21:55 < kanzure> golly is a cellular automata simulator 21:55 < genehacker> ok 21:55 < nsh> http://golly.sourceforge.net/ 21:55 < genehacker> so is that thing on that mans wall a time lapse? 21:56 < nsh> no idea 21:56 < kanzure> on the wall is a printout of the 200,000th iteration 21:56 < genehacker> oh neat 21:56 < kanzure> but it's more interesting to watch it animated 21:56 < kanzure> because you see islands of order and stuff break off and go away.. 21:56 -!- lepton [~lepton@174-29-2-161.hlrn.qwest.net] has joined #hplusroadmap 21:56 < kanzure> i just like watching it and making up stories :P 21:56 < kanzure> "oh and now there's a colony" 21:56 < genehacker> so I wonder if all this cellular automata stuff will actually be useful someday 21:56 < lepton> *old and busted computer here at home 21:57 < kanzure> "oh noes they might be dying" (and then thbey don't- providing a rail/structure for the snowball effect from the bigger former breakaway point to come over and takem the non) 21:57 < genehacker> especially if we make real world cellular automata 21:57 < kanzure> *take them on 21:57 < kanzure> sure, people have done real world cellular automata 21:57 < kanzure> paul rothemund did it on DNA once, i think 21:57 < genehacker> oh snap 21:57 < genehacker> paper link now 21:57 < genehacker> I know that 21:57 < kanzure> paul rothemund did your mom on dna, so whatever- not that impressive ;) he's a wizard 21:58 < kanzure> lemme see 21:58 < genehacker> someone made a glider gun in a cyclic chemical system 21:58 < kanzure> hmm where did i put this paper 21:58 < nsh> i'll be impressed when we get cellular automata processing information in, i don't know, convection cells on the ocean 21:59 < genehacker> what I want to see is a cellular automata machine that can reconfigure itself into different speciallized computers 21:59 < nsh> something massive and physical 21:59 < nsh> i think you run into synch problems in real physical systems over a certain size 21:59 < genehacker> yup 21:59 < kanzure> genehacker: basically it was paul's sierpenski triangles on dna, that was cellular automata. 21:59 < nsh> hard to have a 'clock' in the real world. damn you einstein... 22:00 < nsh> i was reading a while back about emergent properties in asynchronous cellular automata 22:00 < nsh> didn't get any clear conclusions though 22:00 < kanzure> genehacker: http://www.dna.caltech.edu/Papers/SierpinskiDNA_PLoS2004.pdf 22:01 < nsh> this looks interesting: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.7.2073 22:01 < kanzure> Algorithmic Self-Assembly of DNA Sierpinski Triangles http://www.dna.caltech.edu/Papers/SierpinskiDNA_PLoS2004.pdf 22:01 < kanzure> from http://www.dna.caltech.edu/DNAresearch_publications.html 22:01 < genehacker> nsh quantum interactions are expected to be 10^35 times faster than the speed of light 22:01 * nsh doesn't know what that means 22:01 < kanzure> fake 22:01 < genehacker> but only downside is you can only transfer information out of a quantum system at the speed of light... 22:02 < kanzure> figures 22:02 < nsh> i don't know if you can process anything using quantum interactions 22:02 < nsh> i need to study quantum information theory 22:02 < kanzure> nsh: what about dwave? 22:02 < kanzure> the cfompany 22:02 < kanzure> apparently i have significant investments in them O_o 22:02 < kanzure> and the CEO is speaking at h+ summit 22:02 * nsh waits and sees 22:02 < kanzure> if you were quantum you would do your seeing first, and then your waiting 22:02 * nsh smiles 22:03 < genehacker> I have misgivings about putting a lot of money into dwave at this point in time 22:03 < kanzure> i didn't choose to do it :P 22:03 < kanzure> the old man did it before he died 22:04 < nsh> i'm sure something will come of it 22:04 < nsh> just perhaps not what's expected 22:05 < nsh> science is the courtship of serendipity 22:05 < kanzure> apparently the ceo (what's his name?) used their computing platform to solve the scheduling optimization problem for h+ summit 22:05 < nsh> heh 22:05 < genehacker> hopefully a quantum internet and not quantum cryto cracking 22:05 < nsh> oh, i didn't get around to reading Post-quantum cryptography 22:05 < nsh> or whatever it's called 22:06 < nsh> http://www.springer.com/mathematics/numbers/book/978-3-540-88701-0 22:06 < nsh> have it knocking about somewhere 22:06 < kanzure> do you need access to that springer link 22:07 < nsh> nah, i got it already from somewhere 22:07 < nsh> thanks though 22:08 < nsh> "There's plenty of room at the bottom..." 22:09 < kanzure> a lot of the stuff on the http://www.youtube.com/user/kanzure#grid/user/0EB93E6E02E5CF17 playlist looks like it'd fit right in with koyaanisqatsi 22:09 * kanzure is watching http://www.youtube.com/user/kanzure#p/c/0EB93E6E02E5CF17/37/wbDwUWGvwq4 now 22:10 < nsh> hmm 22:14 * nsh goes for a think 22:14 < InkBlob> kanzure, are you successful and famous or something? you're on youtube. 22:14 < InkBlob> how much cash do you rake in ? 22:14 < kanzure> i have some funding for crazy antics going on in here 22:15 < genehacker> none from youtube though 22:15 < kanzure> but i can't fund recurring payments like dojo membership fees ;-) or rent ;-) 22:15 < genehacker> your facebook drug running should pay for that 22:15 < InkBlob> so you have funding = sum of money, but that is not recurring monthly? 22:16 < kanzure> the lump sum of money was a one time funding gig for me 22:16 < kanzure> so once it's gone, it's gone 22:16 < kanzure> and recurring payments are a really really quick way to use it up 22:16 < kanzure> also i'm hesitant to spend money most of the time.. i's wierd 22:16 < kanzure> i was kinda assuming i'd be broke eforever 22:16 < kanzure> *weird 22:17 < kanzure> *forever 22:17 < kanzure> (i'm ssh'ing into another computer, and my home connection is slow, so that's why all these typos are happening) 22:18 < Yocttar> So, any1 here can guide me to the next step after extracting my DNA? I wish to extract some algea DNA, manipulate it and let it reproduce eventually, so... Any1? :) 22:18 < kanzure> algae reproduce on their own- why would you want to extract DNA from algae for reproduction? 22:19 < genehacker> are you trying to do PCR? 22:19 < Yocttar> __manipulate___ 22:19 < Yocttar> ok, PCR then... how ? :) 22:19 < genehacker> manipulate how? 22:19 < kanzure> ah, for manipulation 22:19 < InkBlob> i am conducting a project with my friends that is medical related. If it becomes successful, we can safely gain at least 15,000 per client (it will become service based.) We are looking for a person who is technically apt enough to understand what we are making (a mechanical device) and has enough expertise in biology. 22:19 < kanzure> i guess there's all sorts of methods 22:19 < Yocttar> lets say input some florecent gene or something 22:19 < kanzure> oh, well then you should consider a plasmid method 22:20 < kanzure> InkBlob: what is your project in particular? 22:20 < genehacker> then you don't take the DNA out you put it in 22:20 < InkBlob> The only thing we are worried about is that we are not licensed practioners of medicine. The particular license we need is probably somewhere along the lines of chiropractice. 22:20 < genehacker> I have mechanical skills but I am just an undergrad 22:21 < InkBlob> kanzure, I will speak to you in private after I gather the materials to show you what we are on to. 22:21 < InkBlob> Is that ok with you? 22:21 < kanzure> yes 22:21 < kanzure> are you ok on a phone? 22:21 < InkBlob> Please give me few days. 22:21 < genehacker> is this something that goes inside people? 22:21 < kanzure> speaking of which, someone recommended - anonymously- that we start having weekly conference calls 22:21 < InkBlob> Maybe. I prefer logged chat for later reference. 22:21 < kanzure> for the diybio, hackerspaces, open manufacturing, transhumanism cross-over community 22:22 < InkBlob> genehacker: No. It's for aesthetic purposes, and is non-surgical based. 22:22 < InkBlob> hence why it's more plausible. 22:22 < kanzure> would anyone be interested in joining the teleconferences over skype? 22:22 < kanzure> for some reason, people prefer voice chat for weekly meetings 22:22 < kanzure> and don't take scheduled irc chats seriously (wtf) 22:23 < genehacker> sounds interesting I would like to know more 22:23 < kanzure> in particular i'd like to get you asshats back on the journal club reading schedule 22:23 < kanzure> slackers 22:23 < kanzure> all of you, just slackers. gah 22:23 < genehacker> what schedule? 22:24 < kanzure> weren't we doing a daily/weekly journal club thing? 22:24 < genehacker> not that I know of 22:24 < kanzure> hmm 22:24 < Yocttar> Any guide for plasmid manipulation? 22:24 < kanzure> tons :) 22:25 < Yocttar> well , a simple one? :D 22:25 < kanzure> http://protocol-online.org/ has a ridiculous load of links 22:25 < Yocttar> I theoreticlly know the procedure 22:25 < Yocttar> never in a lab 22:26 < Yocttar> I can build w\e tools necessary 22:27 < kanzure> the equipment for plasmid stuff is usually like a refrigerator, a freezer, petri dishes, an incubator, and more importantly the chemicals 22:28 < kanzure> like for making the organism competent- the most basic version is a calcium chloride protocol 22:28 < kanzure> but there are also methods involving gene guns, electrical techniques, natural competence (i.e. the organism might naturally take up DNA), and so on 22:28 < Yocttar> Well, if that is the most basic method, I should look into it.. 22:29 -!- Alystair [Alystair@bas1-toronto10-1279555684.dsl.bell.ca] has quit [Read error: Operation timed out] 22:29 < kanzure> it depends on your target organism, mind you 22:29 < kanzure> often you have to tweak a protocol for plasmid transfection for a related organism if you're working with something bizarre/non-standard 22:29 < Yocttar> for algea 22:29 < kanzure> yeah algae transfection isn't exactly bio 101, but i don't recall how complicated it is 22:30 < kanzure> most people do transfection with ecoli for starters, or something they can smear on a petri dish 22:30 < Yocttar> ok then 22:30 < kanzure> i'm not saying algae is impossible, just that it's not a typical first genetic engineering project 22:30 < Yocttar> ecoli 22:30 < genehacker> GFP algae? is someone trying to pull a prank on a school by dumping GFP algae into a fountain that happens to be on campus? 22:30 < genehacker> because that sounds like a great idea 22:30 < Yocttar> well 22:30 < Yocttar> actually 22:30 < Yocttar> my starting idea was 22:30 < Yocttar> to put a glowing algea 22:30 < Yocttar> in my fountain 22:30 < Yocttar> wouldnt that be cool? :D 22:30 < genehacker> there's already glowing algae out there 22:31 < genehacker> or glowing bacteria 22:31 < genehacker> can be obtained from REALLY fresh fish 22:31 < kanzure> gfp only fluoresces in the presence of blue light, mind you 22:31 < kanzure> mind you mind you mind you 22:31 < kanzure> i need to stop saying that 22:32 < genehacker> there's this one fountain I know of that uses blue lights 22:32 < genehacker> yes the type that would make algae fluorecse 22:33 < genehacker> anyway making stuff glow in the dark is hard 22:33 < Yocttar> "can be obtained from REALLY fresh fish" , 22:33 < genehacker> yup 22:33 < Yocttar> what do you mean? 22:34 < Yocttar> Lets say I buy a fish 22:34 < Yocttar> ! 22:34 < kanzure> oh no not the samon sperm thing again 22:34 < kanzure> :( 22:34 < Yocttar> a fresh 1 22:34 < Yocttar> O_o 22:34 < kanzure> *salmon 22:34 < genehacker> http://www.sciencemadness.org/talk/viewthread.php?tid=1485 22:34 < genehacker> nope 22:34 < Yocttar> why would any fish have a glowing algea on him 22:35 < genehacker> that's how we'd make synthetic DNA monomers though or some form of LED 22:35 < genehacker> because it happens to live in the ocean 22:35 < genehacker> it's bacteria actually, happens to grow on dead fish 22:36 < genehacker> every seen bioluminescent stuff in the see when you agitate seawater? 22:36 < genehacker> that's mostly that bacteria 22:38 < Yocttar> nope I never saw that ;( 22:38 < genehacker> go to an ocean sometime 22:38 < genehacker> and get a bucket of water and agitate it 22:39 < Yocttar> My fountain has sweet water tough ;( 22:40 < Yocttar> that will work anywhere in the world you say? 22:40 < genehacker> it depends 22:40 < genehacker> sometimes 22:40 < Yocttar> I dont think so =P 22:40 < Yocttar> those bacteria must've taken over the world then 22:41 < genehacker> you don't live in the US do you? 22:41 < genehacker> they are fairly ubiquitous 22:42 < genehacker> such that this can occur: http://weirdnewsfiles.com/wp-content/weirdnewsuploads/milky_sea.jpg 22:42 < genehacker> that's a patch of sea where they all lit up 22:43 < genehacker> yup they are present in all marine environments 22:47 < kanzure> ooh http://www.programmableweb.com/apis/directory 22:47 < Yocttar> Im from Israel 22:48 < genehacker> excellent you have a nice patch of ocean which you can experiment on 22:48 < Yocttar> ;D 22:48 < genehacker> fresh fish should definately be nearby 22:48 < genehacker> I live far too inland to extract luminous bacteria from fish 22:49 < kanzure> good night robot army masquerading as human commandos 22:58 < Yocttar> night ;) 22:58 < Yocttar> (morning here) :) 22:58 < genehacker> there is no such thing as night on the internet! 23:14 -!- Roy78 [~Tiger@ip68-11-187-208.br.br.cox.net] has quit [Ping timeout: 260 seconds] 23:22 < Yocttar> Bah, so easy to find dna extraction process, nothing on insertion :< 23:42 < Yocttar> any helpful forums\boards for extreme lab beginners like me? :p 23:42 < genehacker> it's very specific to what you are working with 23:43 < genehacker> if you really want experience take a class on it 23:43 < genehacker> you could also read kanzure's paper repository 23:43 < genehacker> but taking a class will get you more experience 23:45 < genehacker> you could just go to the diybio google group 23:52 -!- marainein [~marainein@220.253-202-18.VIC.netspace.net.au] has joined #hplusroadmap 23:58 < Utopiah> Spiders at the Nanoscale: Molecules that Behave Like Robots http://cumc.columbia.edu/news/press_releases/DNArobot.html