--- Log opened Fri Jun 24 00:00:07 2011 00:19 -!- Drakkar [~PixelScum@ip98-177-175-88.ph.ph.cox.net] has joined ##hplusroadmap 00:44 < QuantumG> heh.. if I could pick one sentence to describe you forever, it would be that one. 01:31 -!- jenzebubble [~jen@173-27-44-143.client.mchsi.com] has joined ##hplusroadmap 01:34 -!- jennicide [jen@173-27-44-143.client.mchsi.com] has quit [Ping timeout: 252 seconds] 02:28 -!- alystair [alystair@24-246-14-18.cable.teksavvy.com] has quit [Ping timeout: 276 seconds] 02:48 -!- JaredW [~JaredW@118.142.17.238] has quit [Remote host closed the connection] 02:49 -!- JaredW [~JaredW@118.142.17.238] has joined ##hplusroadmap 02:58 -!- Helleshin [~talinck@cpe-71-67-103-109.cinci.res.rr.com] has quit [Read error: Connection reset by peer] 02:58 -!- Helleshin [~talinck@cpe-71-67-103-109.cinci.res.rr.com] has joined ##hplusroadmap 03:40 < fenn> my CIRCLES are beautiful and unique snowflakes 05:10 -!- Jaakko [~Jaakko@host86-131-177-233.range86-131.btcentralplus.com] has joined ##hplusroadmap 05:11 -!- Jaakko is now known as Guest89588 05:30 -!- jenniside [~jen@173-27-44-143.client.mchsi.com] has joined ##hplusroadmap 05:33 -!- jenzebubble [~jen@173-27-44-143.client.mchsi.com] has quit [Ping timeout: 240 seconds] 05:36 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has quit [Remote host closed the connection] 06:06 -!- klafka [~textual@cpe-69-205-70-55.rochester.res.rr.com] has joined ##hplusroadmap 07:20 -!- augur [~augur@208.58.6.161] has quit [Remote host closed the connection] 07:38 -!- panax [panax@131.247.13.196] has joined ##hplusroadmap 07:41 -!- mayko [~mayko@71-22-217-151.gar.clearwire-wmx.net] has joined ##hplusroadmap 07:42 -!- jrayhawk [~jrayhawk@216.151.3.174] has joined ##hplusroadmap 07:56 -!- augur [~augur@129.2.129.34] has joined ##hplusroadmap 08:23 -!- archels [~neuralnet@541ED8B0.cm-5-7d.dynamic.ziggo.nl] has quit [Quit: Reconnecting] 08:24 -!- archels [~neuralnet@541ED8B0.cm-5-7d.dynamic.ziggo.nl] has joined ##hplusroadmap 09:02 < kanzure> synthetic biology conference abstracts: http://sb5.biobricks.org/files/sb5-program-book-v3.pdf 09:12 -!- mayko [~mayko@71-22-217-151.gar.clearwire-wmx.net] has quit [Ping timeout: 255 seconds] 09:14 < kanzure> http://ellingtonlab.org/blog/2011/05/22/on-ribozymes 09:14 < kanzure> http://ellingtonlab.org/blog/2011/04/14/on-designer-chemical-weapons 09:36 -!- panax [panax@131.247.13.196] has quit [Ping timeout: 250 seconds] 09:58 < kanzure> "Translational synthetic biology in insects" 10:32 -!- jebba [~jebba@174-29-84-140.hlrn.qwest.net] has joined ##hplusroadmap 10:48 < kanzure> and less comprehensively: http://sb5.biobricks.org/files/sb5-poster-index.pdf 10:52 < kanzure> hm i didn't realize libsbol and libsbml were different things 10:52 < kanzure> http://www.sbolstandard.org/ 10:54 < augur> kanzure: how did you spend turing day 10:57 < augur> http://blog.regehr.org/archives/546 10:58 < augur> all of this speculative execution stuff seems obvious to me but i suspect thats because im used to thinking about minds in that setting 10:58 < augur> hm 11:02 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 11:11 < kanzure> i like ellefson's poster "Directed evolution of supercharged polymerases" 11:11 < kanzure> oh.. wait that's andy ellington here in austin 11:11 < kanzure> keepin' it real in austin, texas i guess 11:12 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Quit: Leaving] 11:14 < kanzure> "Increasing the surface charge of a protein can alter its properties allowing it to internalize into mammalian cells and be thermal/aggregation resistant." 11:14 < kanzure> i'm not sure what you would do with cell-delivery of polymerases 11:14 < kanzure> worst case scenario you physically inject polymerase into a cell with a puncturing device 11:16 < kanzure> is phage-assisted continuous evolution the same as that recent paper about protein-based directed evolution? 11:19 < kanzure> "It was recently discovered that some bacteria produce organelle-like compartments, contrary to the long-held belief that bacteria lack any such organization. These protein-based bacterial microcompartments (MCPs) encapsulate related enzymes involved in a metabolic process. One such MCP system, found in Salmonella enterica, allows the utilization of 1,2-propanediol as a carbon source." 11:19 -!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap 11:25 < kanzure> "Engineering the next generation of genetically encoded tools using PhytochromeB" is neat.. some sort of two-color system for controlling the conformation of a protein (PhyB) which controls whether it binds/unbinds to a peptide 11:31 < kanzure> hehe "Reconstructing the RNA world: evolution and engineering of improved RNA polymerase ribozymes" describes work done to make R18 RNA polymerase ribozyme capable of template-directed RNA synthesis (up to 105 nt "corresponding to half the length of the ribozyme itself" so not quite done yet) 11:39 < kanzure> i should read 'Replication of Genetic Information with Self-Encoded Replicase in Liposomes' 11:40 < uniqanomaly_> "Supreme Court rules Big Pharma's right to purchase your medical records outweighs doctor-patient privilege. " lmao 11:41 -!- eudoxia [~eudoxia@r190-135-22-51.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 11:41 -!- eudoxia [~eudoxia@r190-135-22-51.dialup.adsl.anteldata.net.uy] has quit [Client Quit] 11:42 -!- eudoxia [~eudoxia@r190-135-22-51.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 11:43 < kanzure> "Error correction of synthetic genes" hrm these guys are claiming an error rate of only 1:4000 in oligonucleotide synthesis 11:46 < kanzure> "Using retrosynthetic biology to design metabolic pathways for therapeutics production" 11:46 < kanzure> "Retrosynthesis, a concept originally proposed for synthetic chemistr,y iteratively applies reversed biotransformations (i.e., reversed enzymes-catalyzed reactions) starting from a target product in order to reach precursors that are endogenous to the chassis." 11:47 < kanzure> "In our method, substrates, products and reactions are coded into molecular signatures and metabolic maps are represented as hypergraphs. The complexity of the retrosynthetic enumeration of all feasible biosynthetic hyperpaths for a given compound [..] can be addressed in our approach by varying the specificity of the molecular signature." 11:48 < kanzure> so.. yeah, retrosynthetic biology. neat. 11:54 < kanzure> an ok but not great overview of dna synthesis in 2009 http://www.duke.edu/~ies2/Publications/Gene%20Synthesis%20Review-Web%20Final.pdf 12:16 -!- augur [~augur@129.2.129.34] has quit [Remote host closed the connection] 12:22 -!- uniqanomaly_ [~ua@dynamic-78-8-216-64.ssp.dialog.net.pl] has quit [Ping timeout: 255 seconds] 12:27 < kanzure> RNA replicase story :) http://en.wikipedia.org/wiki/Spiegelman_Monster 12:35 -!- uniqanomaly_ [~ua@dynamic-78-8-218-201.ssp.dialog.net.pl] has joined ##hplusroadmap 13:01 -!- eudoxia [~eudoxia@r190-135-22-51.dialup.adsl.anteldata.net.uy] has quit [Read error: Connection reset by peer] 13:17 -!- augur [~augur@208.58.6.161] has joined ##hplusroadmap 13:31 -!- eudoxia [~eudoxia@r190-135-109-227.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 13:38 -!- jebba [~jebba@174-29-84-140.hlrn.qwest.net] has quit [Ping timeout: 252 seconds] 14:20 -!- eudoxia [~eudoxia@r190-135-109-227.dialup.adsl.anteldata.net.uy] has quit [Quit: quit] 14:38 -!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Ping timeout: 250 seconds] 15:01 < ybit> supposedly google has these tech talks where they invite a Heath 15:02 < ybit> http://www.youtube.com/watch?v=MVOm9TwM19A :: Heath@Google Series: ... 15:02 < ybit> here's hoping they accept my talk :) 15:20 * ybit was expecting a rimshot 15:20 -!- eudoxia [~eudoxia@r190-135-109-227.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 15:23 -!- Drakkar [~PixelScum@ip98-177-175-88.ph.ph.cox.net] has quit [Quit: The beer is meal.] 15:27 < kanzure> ybit: no rimshots for you 15:27 -!- jebba [~jebba@198.202.202.21] has joined ##hplusroadmap 15:28 -!- jebba [~jebba@198.202.202.21] has left ##hplusroadmap [] 15:42 -!- eudoxia [~eudoxia@r190-135-109-227.dialup.adsl.anteldata.net.uy] has quit [Read error: Connection reset by peer] 15:48 -!- CryptoQuick [~CryptoQui@c-174-51-232-237.hsd1.co.comcast.net] has joined ##hplusroadmap 16:01 -!- jebba [~jebba@198.202.202.21] has joined ##hplusroadmap 16:01 -!- jebba [~jebba@198.202.202.21] has left ##hplusroadmap [] 16:24 -!- eudoxia [~eudoxia@r190-135-109-227.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 16:36 -!- Drakkar [~PixelScum@ip98-177-175-88.ph.ph.cox.net] has joined ##hplusroadmap 16:41 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Ping timeout: 250 seconds] 17:07 -!- Guest89588 [~Jaakko@host86-131-177-233.range86-131.btcentralplus.com] has quit [Quit: Nettalk6 - www.ntalk.de] 18:29 -!- alystair [alystair@24-246-14-18.cable.teksavvy.com] has joined ##hplusroadmap 19:07 -!- PixelScum [~PixelScum@ip98-177-175-88.ph.ph.cox.net] has joined ##hplusroadmap 19:10 -!- Drakkar [~PixelScum@ip98-177-175-88.ph.ph.cox.net] has quit [Ping timeout: 240 seconds] 19:11 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Quit: jmil] 19:59 -!- jenniside is now known as jennicide 20:09 -!- Pabreetzi0 [~Pabreetzi@cpe-174-096-012-143.carolina.res.rr.com] has joined ##hplusroadmap 20:21 < kanzure> hi Pabreetzi0 20:22 < Pabreetzi0> hi bryan 20:23 < Pabreetzi0> hows it going? 20:27 < Pabreetzi0> so whats going on with hplus roadmap? 20:45 -!- eudoxia [~eudoxia@r190-135-109-227.dialup.adsl.anteldata.net.uy] has quit [Quit: quit] 21:29 < Pabreetzi0> so many people here, yet no one talking 21:35 < kanzure> we are busy 21:40 -!- uniqanomaly__ [~ua@dynamic-78-8-84-162.ssp.dialog.net.pl] has joined ##hplusroadmap 21:41 -!- uniqanomaly_ [~ua@dynamic-78-8-218-201.ssp.dialog.net.pl] has quit [Read error: Operation timed out] 21:52 < fenn> we are busy. you will be assimilated. 23:24 -!- klafka [~textual@cpe-69-205-70-55.rochester.res.rr.com] has quit [Quit: Computer has gone to sleep.] 23:41 < kanzure> busy busy busy 23:44 -!- fernan [~pseudo@118.101.154.183] has joined ##hplusroadmap --- Log closed Sat Jun 25 00:00:07 2011