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09:54 < Mokbortolan_> err, a witch's brew of microwave-induced isomerizations? 09:54 < kanzure> i don't know i'm not paranoid enough 09:54 < kanzure> something something about not microwaving plastic 09:55 < Mokbortolan_> I've read all these articles at "health" magazines, but I can't find any real research underlying the claims 09:55 < kanzure> stop reading magazines :P 09:55 < Mokbortolan_> they're the only ones with the chutzpah to challenege the microwave-manufacturer oligarchy! :p 09:56 < Mokbortolan_> I've read about using microwaves in chemistry for a while 09:57 < Mokbortolan_> so I know it does change food chemically... 09:57 < Mokbortolan_> maybe I should do a mouse experiment with my daughter 09:57 < kanzure> you could also consider doing a water microbe project with her 09:58 < kanzure> go to some local water bodies, lakes, streams, oceans and gather some critters 09:58 < kanzure> buy a nice microscope, take some videos 09:58 < Mokbortolan_> yeah! I did that in school 09:58 < Mokbortolan_> egg-yolk and paramecium 09:58 < kanzure> see if you can raise them 09:58 < kanzure> etc. as a kid i would have loved that (i still do) 09:58 < kanzure> i was more of a bug catcher; i didn't know things were that small :( 09:58 < Mokbortolan_> I need to get a decent microscope 09:58 < Mokbortolan_> I read about an adult wasp... smaller than a paramecium 09:58 < foucist> saw something about microwaves being less damaging on food than conventional means of heating, good for steaming veggies etc.. forget where though 09:59 < Mokbortolan_> 0.13mm 09:59 < Mokbortolan_> they hatch (and mate!) inside insect eggs 09:59 < Mokbortolan_> cant' remember what type of eggs, might be bees 10:01 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Read error: No route to host] 10:14 < kanzure> why is dna-directed dna polymerase called "nucleotidyltransferase" 10:14 < kanzure> what does -tidyl- have to do with it 10:17 < uniqanomaly> http://img140.imageshack.us/img140/8797/33108908.jpg nanotech company name idea 10:17 < uniqanomaly> not mine 10:19 < Mokbortolan_> Nigerian Nanotech Inc. 10:19 < Mokbortolan_> ? 10:19 < rkos> maybe it could be used in the movie 2016 http://www.youtube.com/watch?v=iXYmFqEkCGQ 10:23 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 11:09 < kanzure> what about a series of switchable guanylyltransferases 11:10 < kanzure> we'd need 16 switchable enzymes 11:11 < kanzure> poly(n)-adenosine guanylyltransferases, poly(n)-guanine guanylyltransferases, poly(n)-thymine guanylyltransferases, poly(n)-cytosine guanylyltransferases 11:11 < kanzure> unfortunately you would also have to wait for these to dissociate and for the next to attach when you switch your light over 11:12 < kanzure> also, they don't exist. 11:37 -!- sylph_mako [~mako@118-92-47-187.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap 11:44 -!- sylph_mako [~mako@118-92-47-187.dsl.dyn.ihug.co.nz] has quit [Read error: Connection reset by peer] 11:47 -!- delinquentme [~asdfasdf@216.183.186.16] has joined ##hplusroadmap 12:01 -!- sylph_mako [~mako@118-92-47-187.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap 12:04 -!- Juul [~Juul@199.188.193.145] has joined ##hplusroadmap 12:11 < delinquentme> I might have to deploy this on amazon 12:11 < delinquentme> hmmmm! 12:11 < delinquentme> not sure if heroku lets free customers run javer 12:16 < ParahSailin> any way to have google alert for google scholar? 12:18 < kanzure> "A chimeric construct in which loop1 from TdT was inserted in Pol mu displays terminal transferase activity, but a largely reduced DNA-dependent polymerization" 12:18 < ParahSailin> what do you guys think about adhd 12:18 < kanzure> i think adhd is great 12:18 < ParahSailin> not sure if i have it 12:19 < delinquentme> ParahSailin, I think theres a big market for it 12:19 < delinquentme> and if you're a hammer every problem looks like a nail 12:19 < ParahSailin> adderall makes me superman 12:19 < delinquentme> me too 12:20 < delinquentme> but then again any anphetamine does 12:20 < ParahSailin> i mean i can be lazy, and most of the stuff on dsm-iv would describe me 12:20 < ParahSailin> i guess there's just a continuum of "energy level" as a temperamental trait 12:20 < ParahSailin> motivation, akrasia, all that too 12:20 < delinquentme> ParahSailin, i think ADHD is a combination of the fact we've got drugs for focus, parents with health insurance and college being shitty at engaging the students 12:21 < rkos> i don't think you're ever going to get to the end of it if you try to think about whether someone really has a syndrome from the dsm or not 12:21 < ParahSailin> but man the people in #/r/ADHD are pretty hostile to that idea 12:21 < ParahSailin> the raging victimhood 12:21 < kanzure> adhd+adderall is very different from not-adhd+adderall, from what i can tell 12:21 < ParahSailin> "we are different in kind from you, not in degree" 12:21 < delinquentme> ParahSailin, victimhood is awesome 12:21 < delinquentme> its like an extension of religion! 12:21 < delinquentme> im lazy! 12:21 < delinquentme> but its not my FAULT 12:21 < kanzure> ParahSailin: solution, stop reading reddit 12:21 < delinquentme> YESSS 12:21 < rkos> i tend to think that if someone can get some use out of CBT/drugs for their life then thats the only justification you need 12:22 < delinquentme> dont reddit unless r/boobies 12:22 < delinquentme> :P 12:22 < Mokbortolan_> yeah, there are very definite differences in the brains of people diagnosed with adhd and those who do not meet those criteria 12:22 < delinquentme> kanzure, any idea if I can get java on heroku for a rails application? 12:22 < rkos> no need to try to appeal to some god given normal state which you need medical assistance to reach 12:23 < delinquentme> but i mean my life is so much better when things arent my fault 12:23 < kanzure> delinquentme: no i think you have to choose a particular stack on heroku 12:23 < kanzure> delinquentme: stop channeling redditisms in to here.. they aren't useful 12:24 < Mokbortolan_> and the characteristics for ADHD start in childhood and are a common thread throughout a sufferer's life 12:24 < Mokbortolan_> it's basically a developmental disorder 12:24 < Mokbortolan_> AFAIK 12:24 < delinquentme> kanzure, motivation :P 12:25 < delinquentme> but it doesnt work for everyone 12:25 < ParahSailin> i guess "doesn't like to do things that are not very rewarding" is a disease now 12:25 < kanzure> ParahSailin: i think you're talking about different things 12:25 < delinquentme> ParahSailin, so when parents were taught 12:25 < delinquentme> *NOSE TO THE GRINDSTONE* 12:25 < delinquentme> eat shit till it tastes good! 12:25 < ParahSailin> thats pretty much the conclusion i came to on my own, but i went to #/r/ADHD to get the contrary view 12:25 < delinquentme> aka the military model 12:25 < delinquentme> well yeah! 12:25 < kanzure> adhd is presumably caused by some particularly different receptors on neuronal membranes 12:26 < kanzure> any of the psychological explanations of adhd are bullshit 12:26 < delinquentme> who wants to be emotionally raptured when you can just eat shit day in and day out 12:26 < kanzure> it's like trying to explain schizophrenia with psychology 12:26 < rkos> well illness has never been an absolute 12:26 < Mokbortolan_> http://www.citeulike.org/user/xinian/article/1787262 12:26 < kanzure> i think you two are just being drawn into the reddit hype 12:26 < kanzure> so i suggest you stop that.. 12:27 * delinquentme humps Mokbortolan_ 's leg 12:27 * delinquentme mantra: composure 12:27 < delinquentme> Juul, you didnt host your RJB on heroku did you? 12:28 < rkos> but there is no real test for ADHD kanzure 12:28 < kanzure> rkos: you can do sequencing to determine what sort of mutations on receptors you have 12:28 < rkos> so when you talk about ADHD you talk about a very large group of people that aren't defined by something concrete like different receptors 12:29 < kanzure> are you talking about misdiagnosing people? 12:29 < kanzure> or the disorder itsel 12:29 < kanzure> *itself 12:29 < rkos> well sequencing is not used in clinics and certainly not in psychiatry here at least 12:29 * delinquentme sings ke$sha at the top of his lungs 12:29 < delinquentme> ^^^ ADHD 12:30 < ParahSailin> well a video i just watched gave fmri stuff about areas of the brain that dont light up for adhd 12:30 < rkos> isn't ADHD defined by some list of symptoms instead of some concrete feature like the receptor thing? 12:30 < kanzure> rkos: ok so how is that not a real test 12:31 < kanzure> yes it's true that people make mistakes when diagnosing patients.. is that what you want to talk about ?? 12:31 < rkos> i didn't even know there was any test for ADHD 12:31 < ParahSailin> if its a receptor thing, then the only solution is chemical dependency? 12:31 < kanzure> ParahSailin: not necessarily of course 12:32 < Mokbortolan_> "ADHD" will likely be split up into separate disorders in time 12:32 < rkos> but i have a friend who has ADHD and he was just given meds after a few vists and no test 12:32 < kanzure> rkos: to be fair, it's more like "here's a lot of shit that we observe in adhd versus controls" 12:32 < Mokbortolan_> mine is "ADHD - Predominantly Inattentive". I don't have any hyperactivity symptoms, for example 12:32 < ParahSailin> because i'd like to solve my problems, but i dont trust the integrity of most docs 12:32 < kanzure> Mokbortolan_: mine is adhd-hyperactive 12:32 < ParahSailin> or the state of medical science for that matter 12:33 < kanzure> ParahSailin: nor should you 12:33 < Mokbortolan_> you CAN use them to inform your own search for increased performance 12:33 < ParahSailin> i dont know where to look for better info 12:34 < ParahSailin> yah i guess i would be adhd-i as well 12:34 < ParahSailin> what they used to call ADD 12:34 < Mokbortolan_> but I don't buy this whole transfer of responsibility to physicians 12:34 < kanzure> how do you know that's not ADD 12:34 < kanzure> ADD and ADHD definitely have differences 12:34 < ParahSailin> dsm-iv calls add adhd-i 12:35 < kanzure> hmmm is that so 12:35 < ParahSailin> problem with adderall is that it makes me completely emotionally cold 12:35 < Mokbortolan_> the wiki on ADHD-PI is very good 12:35 < kanzure> ParahSailin: that's odd, i'm sorry to hear that 12:35 < Mokbortolan_> adderall kills my creativity 12:35 < Mokbortolan_> and makes me socially weird 12:35 < kanzure> kills your creativity? 12:35 < kanzure> man i must have hit the jackpot 12:35 < Mokbortolan_> yep 12:36 < ParahSailin> i wish i could turn on and off adderall mode for just when i needed it 12:36 < Mokbortolan_> I think (conceptually) it focuses activity, whereas normally I'm calling on regions spread throughout my brain 12:36 < kanzure> also i tend to ignore anything that talks about adhd in terms of dopamine.. dopamine is such a cop-out answer 12:36 < ParahSailin> i dont need to stay awake in the 2s and 3s am 12:36 < kanzure> although talking about particular dopamine receptors might be ok 12:36 < ParahSailin> i just need four hours of high focus work 12:36 < Mokbortolan_> so I can get better at the task at hand, but I can't reference other data useful in conceptual leaps 12:36 < kanzure> ParahSailin: then use the smaller dosages? 12:38 < kanzure> ParahSailin: i really like the enzyme engineering that went into this 12:38 < rkos> or methylphenidate instead of adderall 12:38 < kanzure> ParahSailin: http://diyhpl.us/~bryan/papers2/polymerase/A%20specific%20loop%20in%20human%20DNA%20polymerase%20mu%20allows%20switching%20between%20creative%20and%20DNA-instructed%20synthesis.pdf 12:40 < kanzure> "Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region" 12:41 < kanzure> that's also cool^ 12:43 < kanzure> "Class II enzymes (found in bacteria and eukaryotes) carry a flexible loop in their catalytic core required for switching the specificity of the nucleotide binding pocket from CTP- to ATP-recognition." 12:51 < kanzure> stalk: Tom Hargreaves 12:51 < kanzure> http://sphere.chronosempire.org.uk/~HEx/tmp/prop.pdf 12:51 < kanzure> "Light-dependent RNA polymerase: a research proposal" 12:53 < kanzure> http://sphere.chronosempire.org.uk/~HEx/ 12:54 < kanzure> also he makes sonic music :3 http://sphere.chronosempire.org.uk/~HEx/sonic/ 12:55 < kanzure> looks like he's an ok programmer https://www.google.com/search?num=100&hl=en&q="hex%40freezone.co.uk" 13:01 < ParahSailin> redox might be best way to control an enzyme 13:02 < kanzure> delinquentme: i sent you a rails job email 13:02 < delinquentme> oh hot! 13:02 < Mokbortolan_> http://www.popsci.com/technology/article/2012-02/new-disposable-dna-sequencer-runs-molecular-analysis-powered-usb 13:03 < kanzure> Mokbortolan_: not released yet ;) 13:12 -!- delinquentme [~asdfasdf@216.183.186.16] has quit [Ping timeout: 240 seconds] 13:14 -!- rkos [~chatzilla@a88-113-156-174.elisa-laajakaista.fi] has quit [Remote host closed the connection] 13:43 < archels> 13:43 < archels> Guardian Angels 13:43 < archels> for a smarter life 13:44 < archels> What is this doing behind the logo of the Human Brain Project (www.humanbrainproject.eu)? 13:46 < kanzure> *shrug* lots of insecure servers out there 13:46 < kanzure> page.onLoadFinished = handle_checkout_page_reloading; 13:46 < kanzure> erm.. ignore that 13:47 < kanzure> uhm.. cathal just posted an email: " - To further explore the DIY aspect of eating in the future, the 13:47 < kanzure> butcher Ed Hick will give a live demonstration on how to skin and 13:47 < kanzure> prepare a whole rabbit. www.hicks.ie" 13:55 < roksprok> does that mean all cooking is diybio? 13:55 < roksprok> i guess home brewing is pretty similar anyway 13:56 < Mokbortolan_> if by "diybio" you mean "struggling to survive", then yes! 14:01 < Mokbortolan_> Now who's up for a game of Centrifugal Bumblepuppy?!?! 14:02 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Quit: jmil] 14:10 < archels> Guardian Angels, a project to develop nanoscale sensors and interfaces for detecting and responding to environmental danger. 14:14 < kanzure> is that a lifeboat foundation thing 14:14 < kanzure> archels: how's your protein engineering / rational desig 14:14 < kanzure> design 14:18 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 272 seconds] 14:21 < archels> kanzure: practially zero, I'm trying to engineer neural networks instead. 14:31 < ParahSailin> whats your protein engineering thing 14:33 -!- SDr [~SDr@unaffiliated/sdr] has joined ##hplusroadmap 14:41 < kanzure> hrmm short-patch compartmentalized self-replication 14:45 -!- augur [~augur@129.2.129.34] has quit [Remote host closed the connection] 15:03 -!- SDr [~SDr@unaffiliated/sdr] has quit [Ping timeout: 240 seconds] 15:16 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has joined ##hplusroadmap 15:24 -!- Juul [~Juul@199.188.193.145] has quit [Quit: Leaving] 15:30 -!- jmil_ [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has joined ##hplusroadmap 15:31 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has quit [Ping timeout: 260 seconds] 15:31 -!- jmil_ is now known as jmil 15:43 -!- kvltist [~Kvltist@p5B33E95C.dip.t-dialin.net] has quit [Quit: Computer has gone to sleep.] 15:47 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has quit [Quit: jmil] 15:50 -!- ThomasEgi_ [~thomas@pppdyn-0f.stud-ko.rz-online.net] has joined ##hplusroadmap 15:51 -!- bkero_ [~bkero2@li280-127.members.linode.com] has joined ##hplusroadmap 15:52 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 15:54 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Ping timeout: 252 seconds] 15:54 -!- bkero [~bkero2@li280-127.members.linode.com] has quit [Ping timeout: 252 seconds] 16:03 < foucist> anyone here into evolutionary computation ? 16:04 < kanzure> i've dabbled a bit with GAs and evolutionary search 16:07 < foucist> cool 16:07 < foucist> kanzure: btw, you know of a rails job? 16:07 < kanzure> yeah a few.. 16:08 < kanzure> need something? 16:08 < kanzure> *need one? 16:15 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has joined ##hplusroadmap 16:19 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has joined ##hplusroadmap 16:22 -!- Netsplit *.net <-> *.split quits: ziyadb, drazak, ivan`, strages_home 16:23 -!- Netsplit over, joins: ivan` 16:28 -!- strages_home [~strages@adsl-98-67-175-14.shv.bellsouth.net] has joined ##hplusroadmap 16:28 -!- drazak [~ahdfadkfa@drazak.net] has joined ##hplusroadmap 16:28 -!- ziyadb [u4806@gateway/web/irccloud.com/x-bgcunmonzrupjpmb] has joined ##hplusroadmap 16:30 -!- bkero_ [~bkero2@li280-127.members.linode.com] has quit [Changing host] 16:30 -!- bkero_ [~bkero2@osuosl/staff/bkero] has joined ##hplusroadmap 16:33 -!- yottabit [~heath@unaffiliated/ybit] has joined ##hplusroadmap 16:38 -!- bkero_ is now known as bkero 16:55 -!- n_bentha [~lolicon@75.111.160.104] has joined ##hplusroadmap 17:01 < kanzure> http://news.diybio.org/ 17:01 < kanzure> welp there goes http://bit.ly/diybionews2 17:02 < n_bentha> hey kanzure 17:03 < n_bentha> is that group about sequencing dna using enzymes instead of chemical anaylsis? 17:04 < kanzure> it's not sequencing. synthesis. 17:04 < kanzure> and yes.. using an enzyme 17:05 < n_bentha> oh. ribosomes have u beat 17:06 < kanzure> no ribosomes do their own thing 17:06 < n_bentha> oh dna synthesis 17:06 < n_bentha> i know ribosomes are different, sorry 17:06 < n_bentha> dna pol 3, right? 17:06 < kanzure> ribosomes aren't under direct human control 17:06 < kanzure> polymerase isn't either 17:06 < kanzure> there is no particular polymerase picked out yet 17:06 < kanzure> but there are many candidates (about 3000) 17:07 < n_bentha> so u want to be able to synthesize dna de-novo? 17:07 < kanzure> i want to control dna polymerase's activity with either light, electricity or mechanical mechanisms 17:07 < n_bentha> instead of making oligio's? 17:07 < kanzure> *optical 17:07 < n_bentha> ah cool 17:08 < kanzure> *electrial 17:08 < kanzure> *electrical 17:08 < n_bentha> so flash one wave length and have it add some A's, and another wavelength and have it at some T's? 17:08 < kanzure> it doesn't have to be light, but yes. 17:09 < n_bentha> ah that would be cool. but wouldn't rearrangement of existing sequences combined w/ oligios work better? 17:09 < kanzure> what? 17:09 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has quit [Quit: Leaving] 17:09 < kanzure> are you a biologist? 17:09 < n_bentha> yes 17:09 < kanzure> ok so you're talking about ligating things together? 17:09 < n_bentha> of course 17:10 < kanzure> ok yes i agree ligation is a useful mechanism 17:10 < kanzure> we've developed a ligation scheme that we want to test (in this channel) 17:10 < kanzure> but it's unrelated to the enzymatic synthesis roadmap 17:10 < n_bentha> oooh cool 17:10 < n_bentha> i guess i just don't stand the purpose of the enzymatic synth 17:11 < kanzure> dna polymerase is many thousands of times more efficient than chemical oligo synthesis 17:11 < n_bentha> true 17:11 < kanzure> and in the time that it takes you to access your oligo library for ligation, it will have already synthesized whatever it was from your library that you were trying to fetch ;) 17:12 < n_bentha> ah ok i see what you're saying. 17:13 < n_bentha> it seems like a space-pen situation to me (so much effort to make a pen that writes in space when the russians just used pencils), but i'm a conscientious objector so don't let me discourage the endeavor 17:14 < kanzure> this enzyme already exists 17:14 < kanzure> it's just doing its own thing. 17:14 < n_bentha> oh u want to modify it 17:14 < n_bentha> cool 17:14 < kanzure> are you sure you're a biologist :x 17:15 < kanzure> i mean, polymerase definitely exists 17:15 < n_bentha> yes 17:17 < n_bentha> but haven't many complex sequences arisen from errors in replication? 17:18 < n_bentha> resulting in rearrangements of sequences 17:19 < kanzure> some polymerases have better fidelity and error correction than others. for instance, some have a 1-in-10,000 error rate. 17:21 < n_bentha> of course 17:22 < n_bentha> i wasn't necessarily talking about those kinds of errors, but was just saying that new sequences have come about through errors 17:23 < n_bentha> so it's unlikely that there's an enzyme that'll be able to link specific amino acids together 17:23 < kanzure> we're not linking amino acids 17:24 < n_bentha> nucleotides* 17:24 < kanzure> well, what about CCA-adding enzyme.. seems like that exists to me.. 17:29 < n_bentha> maybe... 17:40 -!- yottabit [~heath@unaffiliated/ybit] has quit [Quit: Konversation terminated!] 17:51 -!- srangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 17:53 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 245 seconds] 17:53 -!- srangewarp is now known as strangewarp 18:05 -!- d3nd3 [~dende@cpc10-croy17-2-0-cust245.croy.cable.virginmedia.com] has quit [Quit: Leaving.] 18:10 -!- delinquentme [~asdfasdf@c-98-219-188-137.hsd1.pa.comcast.net] has joined ##hplusroadmap 18:31 < delinquentme> lawl 18:31 < delinquentme> thank god for headphones 18:31 < delinquentme> music. it makes you soar 18:31 -!- yashgaroth [~f@cpe-24-94-5-223.san.res.rr.com] has joined ##hplusroadmap 18:31 < delinquentme> and it also makes you want to rip faces 18:32 < delinquentme> thank god im not ripping faces w this terribul coffee shop muzak 18:39 < mag1stra1e> delinquentme: How did the interviews go? 18:39 < delinquentme> mag1stra1e, not bad! but I want everything to happen faster 18:40 < delinquentme> got 2 more tomorrow 18:40 < delinquentme> maybe 3 so well see 18:40 -!- n_bentha [~lolicon@75.111.160.104] has left ##hplusroadmap [] 18:41 < mag1stra1e> thats great man! 18:42 < mag1stra1e> How was the one job you really wanted to do? 18:42 < mag1stra1e> Did they give you a pay scale for it yet? 18:46 < kanzure> yashgaroth: so i was reading something today about chimeric polymerases 18:47 < yashgaroth> like those modified high-fidelity/temp ones? 18:47 < kanzure> nope 18:48 < kanzure> http://diyhpl.us/~bryan/papers2/polymerase/A%20specific%20loop%20in%20human%20DNA%20polymerase%20mu%20allows%20switching%20between%20creative%20and%20DNA-instructed%20synthesis.pdf 18:50 < delinquentme> mag1stra1e, nahh man! no payscales yet 18:51 < yashgaroth> it seems like if you can decouple the processivity of the pol from the phosphate being released, you could add one at a time, if you then allow it to proceed with an external signal 18:51 < kanzure> the polymerase motor is sorta well studied, but yeah that's still an unknown 18:51 < yashgaroth> but like with all of this, you'll need to be significantly more reliable than chemical synthesis 18:51 < kanzure> i'm more worried about switching nucleotide selectivity 18:52 < mag1stra1e> delinquentme: have you interviewed with that company yet? 18:52 < yashgaroth> are we still focused on washing in the nucleotide for each step, or using transfer-RNA-like adaptors that can be activated at will 18:52 < kanzure> i don't think i've seen any engineered protein with 4+ chromophores or other light/mechanical/electrical inducable confomational change 18:52 < kanzure> washing is not ok in my opinion 18:52 < kanzure> washing significantly defeats the purpose 18:53 < yashgaroth> okay good cuz that'll be unreliable 18:53 < delinquentme> mag1stra1e, yeahh the LBL one phone interview .. the dude said he was impressed but whoknows 18:53 < delinquentme> payrate could go either way as they're closely coupled bc being a *HUGE* lab << lots of gov funding 18:53 < delinquentme> but they're also .. gov funded 18:54 < yashgaroth> but yeah if you're trying to get one molecule with four light-selectable motifs to add each base, and only doing one at a time, you might as well just design one from scratch 18:54 < mag1stra1e> delinquentme: I really hope it works out for you! 18:54 < kanzure> yashgaroth: i don't even know how that would look. 18:54 < yashgaroth> the elongation is pretty energetically favorable, and all pol does is make sure it matches the template, which we're not doing anyway 18:54 < mag1stra1e> it could be promising if he really likes you 18:54 < mag1stra1e> Just got to keep your hopes up I guess lol 18:55 < delinquentme> mag1stra1e, keep applying is what it is :D 18:55 < yashgaroth> oh man I have no idea how it'd look either, but we're modifying pol so heavily that it'll be a totally new structure anyway 18:55 < kanzure> well no i mean.. if you have 4 nucleotide binding pockets, where do they go 18:56 -!- marainein [~marainein@114-198-70-108.dyn.iinet.net.au] has quit [Ping timeout: 260 seconds] 18:56 < yashgaroth> oh, I was hoping one carrier protein for each nucleotide 18:56 < kanzure> i'd rather only interface with 1 giant enzyme 18:56 < mag1stra1e> :) 18:56 < yashgaroth> complexes, man, nature loves a good complex 18:57 < kanzure> what are you going to do, wait for your laser to transfer enough energy to all the nucleotide-protein complexes? 18:57 < yashgaroth> where did laser come in 18:57 < kanzure> huh? 18:57 < kanzure> what mechanism are you talking about? 18:57 -!- n_bentha [~lolicon@75.111.160.104] has joined ##hplusroadmap 18:58 < yashgaroth> a laser works, but I was thinking more diffuse light, unless you're doing a thousand different reactions in a small area, which I guess you plan to 19:00 < kanzure> there's a lot of ADP/GTP/CTP-binding enzymes that have pretty good selectivity 19:00 < kanzure> we could just steal binding pockets from them 19:02 < delinquentme> 2012-02-23T03:01:31+00:00 heroku[web.1]: State changed from starting to crashed 19:02 < kanzure> congratulations you probably have a syntax error 19:02 < kanzure> did you run it locally first 19:03 -!- n_bentha [~lolicon@75.111.160.104] has left ##hplusroadmap [] 19:03 < delinquentme> kanzure, thats.. 19:03 < delinquentme> not a bad idea 19:05 < delinquentme> 2012-02-23T03:01:26+00:00 app[web.1]: Exception in thread "main" java.lang.NoClassDefFoundError: org/jruby/Main 19:05 < delinquentme> dis 19:08 < delinquentme> ok so I've verified that it downloaded that file correctly... 19:18 < delinquentme> kanzure, is this if fi stuff shell scripts 19:18 < delinquentme> ? 19:20 < kanzure> where are you looking 19:24 -!- delinquentme [~asdfasdf@c-98-219-188-137.hsd1.pa.comcast.net] has quit [Quit: Leaving] 19:36 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has joined ##hplusroadmap 19:43 -!- jmil [~jmil@c-69-249-188-134.hsd1.nj.comcast.net] has quit [Quit: jmil] 19:45 -!- delinquentme [~asdfasdf@70-90-236-161-Pennsylvania.hfc.comcastbusiness.net] has joined ##hplusroadmap 20:04 -!- delinquentme [~asdfasdf@70-90-236-161-Pennsylvania.hfc.comcastbusiness.net] has quit [Ping timeout: 240 seconds] 20:17 -!- delinquentme [~asdfasdf@64.134.64.207] has joined ##hplusroadmap 20:28 < delinquentme> sometimes I feel like I pitch some heavy shit in my emails hahah 20:29 < delinquentme> kanzure, the email attached to that position you forwarded is busted 20:30 < kanzure> just email whoever sent it 20:31 < delinquentme> jring@sharedhr.com is in the from field 20:33 < kanzure> yeah just email that person 20:36 < delinquentme> got it sorted 20:36 < delinquentme> thanks for the heads up 20:38 < delinquentme> NAO 20:38 < delinquentme> we interface w the heorku 20:38 < delinquentme> NO! 20:38 < delinquentme> t3h sinatra 20:38 -!- yottabit [~heath@unaffiliated/ybit] has joined ##hplusroadmap 21:04 -!- delinquentme [~asdfasdf@64.134.64.207] has quit [Ping timeout: 245 seconds] 21:04 -!- zacharycohn_ [~zacharyco@ec2-175-41-214-17.ap-northeast-1.compute.amazonaws.com] has joined ##hplusroadmap 21:17 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 265 seconds] 21:17 -!- ThomasEgi_ [~thomas@pppdyn-0f.stud-ko.rz-online.net] has quit [Remote host closed the connection] 21:28 -!- JayDugger [~duggerj@pool-173-74-78-36.dllstx.fios.verizon.net] has joined ##hplusroadmap 21:28 -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap 21:30 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has joined ##hplusroadmap 21:32 -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 265 seconds] 21:33 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has quit [Quit: Leaving] 21:38 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Quit: leaving] 21:39 -!- JayDugger [~duggerj@pool-173-74-78-36.dllstx.fios.verizon.net] has quit [Quit: Leaving.] 21:47 -!- yottabit [~heath@unaffiliated/ybit] has quit [Ping timeout: 276 seconds] 22:07 < sylph_mako> So, how about that google HUD thing. 22:08 < sylph_mako> Personally I'm underwhelmed but I still desperately need an optical uplink and I'll take what I can get. 22:10 < zacharycohn_> sylph_mako: it's an early look at the v1. 22:10 < zacharycohn_> I'm pretty excited, I will probably get one 22:10 < sylph_mako> It is very exciting. To see something like that finally being pushed mainstream. 22:13 -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has joined ##hplusroadmap 22:15 -!- roksprok [~Zac@74.83.205.124] has quit [Ping timeout: 255 seconds] 22:20 -!- srangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 22:23 -!- srangewarp is now known as strangewarp 22:24 < Stee|> sylph_mako, link? 22:26 < sylph_mako> Stee|, http://bits.blogs.nytimes.com/2012/02/21/google-to-sell-terminator-style-glasses-by-years-end/ 22:38 < Stee|> good 22:39 < Stee|> that'll help development efforts 22:39 < kanzure> help what? 22:40 < Stee|> we're working with some sixthsense stuff on my site 22:40 < Stee|> this makes a nice neat package for that 22:46 -!- uniqanomaly [~ua@dynamic-78-8-214-230.ssp.dialog.net.pl] has quit [Ping timeout: 245 seconds] 22:52 -!- uniqanomaly [~ua@dynamic-78-8-214-230.ssp.dialog.net.pl] has joined ##hplusroadmap 23:04 -!- yashgaroth [~f@cpe-24-94-5-223.san.res.rr.com] has quit [Quit: Leaving] 23:07 -!- JayDugger [~duggerj@pool-173-74-78-36.dllstx.fios.verizon.net] has joined ##hplusroadmap 23:07 < sylph_mako> Could I get a link to that stuff Stee|? 23:11 * kanzure sleeps 23:13 < Stee|> sylph, the convo? 23:13 < Stee|> we're just getting started now 23:15 * Mokbortolan_1 designs a brain input system. 23:15 < Stee|> Mokbortolan_1, go post it! 23:15 < Mokbortolan_1> post what? 23:15 < Mokbortolan_1> I'll post the kickstarter link when it's ready :p 23:16 < Stee|> your dev efforts 23:16 < Mokbortolan_1> oh 23:16 < Mokbortolan_1> well, it'll be proprietary 23:16 < Stee|> bah, fair 23:16 < Stee|> talked to rdb? 23:16 < Mokbortolan_1> at least, the hardware stuff 23:16 < Mokbortolan_1> there'll be an open source SDK 23:16 < Stee|> how much cash are you going to need? 23:17 < Mokbortolan_1> I have no idea 23:17 < Stee|> any idea when you're going live with this? 23:17 < Mokbortolan_1> could be $20,000, could be $2,000,000 23:17 < Mokbortolan_1> it'll take 1-2 years minimum 23:17 < Mokbortolan_1> the goal is to give my friend some new eyes 23:17 < Mokbortolan_1> he's going blind 23:18 < Mokbortolan_1> and he's too cool to let him wander around with a cane 23:21 < sylph_mako> Stee|, the sixthsense stuff? I thought sixthsense was the lanyard projector thing, not really designed for a HUD. 23:23 -!- delinquentme [~asdfasdf@c-67-171-66-113.hsd1.pa.comcast.net] has quit [Ping timeout: 240 seconds] 23:25 < sylph_mako> Erm. The chest projector. 23:25 < Stee|> sylph: just imagine instead of it projecting out on something, it instead simply puts that video output onto the hud 23:35 -!- srangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 23:37 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 245 seconds] 23:37 -!- srangewarp is now known as strangewarp 23:52 -!- zacharycohn_ [~zacharyco@ec2-175-41-214-17.ap-northeast-1.compute.amazonaws.com] has quit [Ping timeout: 272 seconds] 23:53 -!- zacharycohn [~zacharyco@ec2-107-20-74-248.compute-1.amazonaws.com] has joined ##hplusroadmap --- Log closed Thu Feb 23 00:00:14 2012