--- Log opened Tue Jul 24 00:00:17 2012 00:31 -!- SolG [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap 00:33 -!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 260 seconds] 00:53 -!- sylph_mako [~mako@39.19.252.27.dyn.cust.vf.net.nz] has quit [Quit: Leaving] 00:55 -!- splicer [~ubuntu@c83-255-190-140.bredband.comhem.se] has joined ##hplusroadmap 01:04 -!- qnm_ [~robsharp@1.138.176.222] has joined ##hplusroadmap 01:12 -!- qnm_ [~robsharp@1.138.176.222] has quit [Ping timeout: 248 seconds] 01:57 -!- klafka [~textual@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Read error: Connection reset by peer] 01:57 -!- klafka [~textual@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 02:00 -!- AdrianG [~ircname@unaffiliated/amphetamine] has quit [Read error: Connection reset by peer] 02:06 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Ping timeout: 240 seconds] 02:15 < Utopiah> http://edge.org/conversation/biology-at-the-speed-of-light 02:31 -!- AdrianG [~ircname@unaffiliated/amphetamine] has joined ##hplusroadmap 02:57 -!- shin-to [~shin-to@ulmg-5d847fc4.pool.mediaWays.net] has joined ##hplusroadmap 02:57 -!- kvltist [~Kvltist@p5B33F5CC.dip.t-dialin.net] has joined ##hplusroadmap 03:15 -!- kvltist [~Kvltist@p5B33F5CC.dip.t-dialin.net] has quit [] 03:22 -!- qnm_ [~robsharp@2001:44b8:3110:f300:b541:3c68:9646:8694] has joined ##hplusroadmap 03:26 -!- qnm_ [~robsharp@2001:44b8:3110:f300:b541:3c68:9646:8694] has quit [Client Quit] 03:48 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 04:04 -!- ThomasEgi [~thomas@gw-ko-kostr2.inka-online.net] has joined ##hplusroadmap 04:04 -!- ThomasEgi [~thomas@gw-ko-kostr2.inka-online.net] has quit [Changing host] 04:04 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 04:25 -!- SolG is now known as _sol_ 04:41 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Read error: Operation timed out] 04:43 -!- shin-to [~shin-to@ulmg-5d847fc4.pool.mediaWays.net] has quit [Quit: systems shutting down_shin-to is entering sleepmode] 04:53 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap 04:58 -!- ThomasEgi [~thomas@gw-ko-kostr2.inka-online.net] has joined ##hplusroadmap 04:58 -!- ThomasEgi [~thomas@gw-ko-kostr2.inka-online.net] has quit [Changing host] 04:58 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 05:16 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 06:56 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Quit: A cardboard cutout of Bill Murray, a tangerine with a face drawn on it, and a rough draft of a Harry Potter/Twilight crossover fanfic.] 07:11 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 07:15 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 07:33 -!- klafka [~textual@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Quit: Computer has gone to sleep.] 08:01 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 08:03 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 08:12 < strangewarp> Aha 08:12 -!- HEx1 [~HEx@212.159.112.196] has quit [Ping timeout: 252 seconds] 08:13 -!- HEx1 [~HEx@212.159.112.196] has joined ##hplusroadmap 08:14 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has joined ##hplusroadmap 08:15 < strangewarp> I wish PZ Myers were less confrontational, because he makes some decent points, but then all his fans get whipped up into a splendour of strawman-beating.. 08:15 < strangewarp> Regardless, have a thing: http://scienceblogs.com/pharyngula/2012/07/14/and-everyone-gets-a-robot-pony/ 08:20 -!- calango [~dnm@pdpc/supporter/active/calango] has joined ##hplusroadmap 08:21 -!- calango [~dnm@pdpc/supporter/active/calango] has quit [Client Quit] 08:46 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 08:46 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Client Quit] 08:55 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has quit [Ping timeout: 260 seconds] 09:00 -!- rafael [~dnm@pdpc/supporter/active/calango] has joined ##hplusroadmap 09:02 < kanzure> rafael: hi 09:02 < rafael> hi kanzure 09:13 < Vicarious> hi 09:13 -!- rmcl [~rmcl@rrcs-108-178-168-32.west.biz.rr.com] has joined ##hplusroadmap 09:17 -!- azonenberg [~azonenber@2001:470:888b:2:206:70ff:fe01:46] has quit [Ping timeout: 245 seconds] 09:37 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap 09:40 < delinquentme> Anyone know offhand the RUBY string interpolation thats python-esque? puts "ops %s %s" % ( busted[i-1], busted[i+1] ) 09:40 < delinquentme> something like that 09:45 < kanzure> http://gen9bio.com/g-prize/ 09:45 < kanzure> "Gen9 has developed a unique technology to synthesize DNA constructs and has used it to build a novel fabrication capability for next-generation gene synthesis known as the BioFab® platform. The BioFab® platform has the capacity to generate tens of thousands of synthetic gene fragments per year in just a few square feet of laboratory space." 09:45 < kanzure> "Today, Gen9 is manufacturing and shipping double-stranded GeneBits™ DNA constructs, or gene fragments, from 500 to 1,024 base pairs long." 09:45 < kanzure> "If you also believe in the potential of synthetic biology, tell us how you want to revolutionize an industry through your research – you could win up to 500 synthetic DNA constructs (GeneBits™) to pursue your big idea." 09:48 < delinquentme> yar! 09:48 < delinquentme> cool ish eh? 09:48 < delinquentme> i mean its smart ish advertising-ish on their part 09:49 < kanzure> not cool at all 09:50 < kanzure> just something i saw. 09:50 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 09:50 < delinquentme> you dont like it? 09:50 < kanzure> i am surprised they are calling it biofab 09:50 < kanzure> since endy just got done with biofab w/ juul 09:51 < delinquentme> juul is working w endy?? 09:51 < delinquentme> lucky ass dude. 09:51 < kanzure> has been for a long time 09:52 < delinquentme> hes not here 09:52 < delinquentme> i was gonna tell him that I hate him. 09:52 < kanzure> you hate him now? 09:52 < delinquentme> kanzure, you want to start a GFP poison dart frog business w me? 09:53 < kanzure> no 09:53 < delinquentme> no_me_gusta.gif 09:53 < delinquentme> modified puppys? 09:53 -!- nmz787 [~Nathan@72-61-221-154.pools.spcsdns.net] has joined ##hplusroadmap 09:54 < kanzure> yo nmz787 09:54 < kanzure> nmz787: http://gen9bio.com/g-prize/ 09:54 < kanzure> delinquentme: linking does not imply endorsement 09:55 < delinquentme> kanzure, i just like frogs 09:56 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 09:56 < nmz787> hmm 09:57 < nmz787> "A panel of judges which includes, amongst others, David Baker, Sri Kosuri and Christopher Emig has been chosen by Gen9" 09:57 < nmz787> kanzure: kosuri was mentioned on that phone interview with syngenomics 09:58 < nmz787> he did some high-fidelity pooled oligo synthesis 10:00 -!- Netsplit *.net <-> *.split quits: chris_99, splicer, Mokbortolan_1 10:02 -!- Netsplit over, joins: splicer 10:03 -!- Netsplit over, joins: chris_99 10:05 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 10:28 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 252 seconds] 10:29 < nmz787> this might be decent to have around http://twitter.com/SynBioNews/ 10:30 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 10:49 -!- azonenberg [~azonenber@2001:470:888b:2:206:70ff:fe01:46] has joined ##hplusroadmap 11:11 < nmz787> hi azonenberg 11:11 < azonenberg> hi 11:11 < nmz787> you were doing some kind of lithography, or am I wrong? 11:13 < nmz787> was it with visible light? 11:13 < nmz787> did you have a beam expander? 11:16 < nmz787> ahh you're doing homecmos 11:24 < kanzure> nmz787: hmm i got an email from him. Sriram Kosuri 11:28 < kanzure> forwarded some things. 11:29 < delinquentme> http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/ << Stacey K pushed out her Open PCR 11:32 < kanzure> ugh instructables.com 11:33 < kanzure> well. at least she posted it. :( 11:33 < kanzure> http://staceyk.org/pcr/pcrFinal.ino 11:37 < delinquentme> you think thingverse woulda been better? 11:37 < nmz787> kanzure: know anyone here http://www.beatrizdacosta.net/wetwarehackers_site/ 11:39 < kanzure> delinquentme: i think software should be in software repositories 11:39 < kanzure> damn, stacey isn't giving me a name for this 11:39 < kanzure> how am i supposed to name the git repo 11:39 < kanzure> nonsucky-openpcr.git? 11:39 < kanzure> arduino-thermal-cycler.git is a bit generi 11:39 < kanzure> generic 11:43 < delinquentme> OpenPCR_gen2 11:43 < delinquentme> OpenPCR_build2 11:44 < nmz787> kanzure: there are seemingly a lot of cool diybio instructables... http://www.instructables.com/id/Paper-disk-DNA-preparation/ http://www.instructables.com/id/DIY-StirrerHot-Plate/ 11:44 < kanzure> https://github.com/kanzure/wiremound-pcr 11:46 < kanzure> also backed up here http://diyhpl.us/cgit/ 11:46 < delinquentme> nmz787, theres a fusion reactor on there :D 11:46 < nmz787> kanzure: seems like time you started linkdumping on DIYbio again... lots of new folks there now that want info 11:47 < kanzure> nmz787: or it's time people actually contribute to http://openwetware.org/wiki/DIYbio/FAQ 11:47 < kanzure> a lot of content is missing 11:48 < kanzure> and it should probably be moved off of openwetware, since nobody has an account there 11:48 < nmz787> i was just signing up for the OWW mailing lists :P 11:48 < delinquentme> moved to where kanzure 11:48 < kanzure> wtf 11:48 < nmz787> i've had an account there ... was thinking it seems pretty stable 11:48 < kanzure> delinquentme: well, probably http://diyhpl.us/wiki 11:48 < nmz787> and unhacked AFAIK 11:48 < kanzure> nmz787: but people need to wait to get an account 11:48 < delinquentme> and what has jcline been up to? 11:48 < kanzure> so, wtf, i'm looking at the "FAQ Revision History" 11:48 < kanzure> and it says "1.0 - copied on 4/7/2009 from heybryan.org...DIYbio_FAQ" 11:48 < kanzure> wut. 11:48 < nmz787> lol 11:49 < nmz787> daym 11:49 < nmz787> i mean duh-yam 11:49 < kanzure> one of the videos is dead on that page 11:49 < kanzure> nobody has fixed that in a year 11:49 < nmz787> kanzure: why did you call it wiremound? 11:50 < kanzure> nmz787: that's what staceyk said the name was 11:50 < kanzure> when i asked her. 11:50 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Quit: Leaving] 11:55 < kanzure> nmz787: i definitely think a lot of this needs to be rewritten 11:55 < nmz787> can we rip the instructable? did you ask her that? 11:56 < kanzure> didn't ask her, she claims that she is bbl-ing at the moment 11:57 < kanzure> though she still looks online 11:58 < nmz787> bbl? 11:58 < nmz787> oh 11:58 < kanzure> you know.. brb, bbl. 11:58 < nmz787> yeah yeah 12:22 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 264 seconds] 12:36 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 12:43 < kanzure> "My former coworker would use the mouse to copy and paste even 3-letter variable names rather than typing them in again. He'd also scroll his terminal window up and copy and paste old commands (again, all with the mouse) rather than using the up arrow, even after I told him about command line history and tab completion in case he didn't know," 12:43 < kanzure> "and would frequently mis-copy and miss the first letter off or have a stray $ at the front, causing him to start his mousing over from scratch rather than using the keyboard to correct it." 12:44 * Urchin facepalms 12:44 < Urchin> though it does remind me of when I first started using Emacs 12:45 < nmz787> i dont know emacs 12:46 < nmz787> should I? 12:46 < Urchin> I was doing something repetitive that could be done esily with the regular keyboard commands, and at one point half-way trough (after several minutes), I said "what am I doing?" and finished the job in couple of seconds 12:47 < Urchin> the admin that was watching me behind my back said he was asking the same question 12:47 < Urchin> nmz787: it's a traditional unix text editor 12:47 < Urchin> one of 12:47 < Urchin> and one of the two that started religions 12:48 < Urchin> though that was just the effect of me being a complete beginner at it 12:49 < Urchin> it takes a while to learn and master 12:49 < Urchin> I still reach for the manual daily 12:52 -!- Helleshin [~talinck@cpe-174-101-208-182.cinci.res.rr.com] has joined ##hplusroadmap 12:57 < kanzure> nmz787: personally i use vim 12:57 < kanzure> so if you choose emacs then i can't help you as much 13:00 < nmz787> good enough 13:00 < nmz787> ttyl 13:04 -!- nmz787 [~Nathan@72-61-221-154.pools.spcsdns.net] has quit [Ping timeout: 248 seconds] 13:05 -!- srangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 13:06 < Urchin> given how much vim is bloated these days, it's mostly down to the question of interface and what you're used to 13:07 < kanzure> well clearly the correct solution is the emacs emulation of the vim emulation of emacs 13:07 < Urchin> nah, doesn't work 13:08 < Urchin> just use whatever 13:08 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 255 seconds] 13:08 -!- srangewarp is now known as strangewarp 13:11 -!- splicer [~ubuntu@c83-255-190-140.bredband.comhem.se] has quit [Ping timeout: 255 seconds] 13:19 -!- obscurite [~obscurite@danielpacker.org] has joined ##hplusroadmap 13:20 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 13:23 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap 13:24 < obscurite> have you folks been talking about the stanford whole cell simulation at all? 13:24 -!- splicer [~ubuntu@c83-255-190-140.bredband.comhem.se] has joined ##hplusroadmap 13:24 < obscurite> (mycoplasm genitalia, simplest bacteria) 13:25 < archels> obscurite: will attempt to interest my labmates for it tomorrow. 13:25 < yashgaroth> needs more quotation marks around simulation 13:25 < obscurite> archels: http://wholecell.stanford.edu/ 13:25 < archels> I read it, thanks 13:25 < obscurite> yashgaroth: har har :P 13:26 < obscurite> archels: any thoughts? 13:26 < archels> obscurite: dunno, a tad underwhelming? 13:26 < obscurite> archels: really? seems to break new ground, even if they recycled the pathways 13:27 < archels> the task of cobbling this together was obviously the size of a mammoth 13:27 < archels> but what fundamental grounds is broken, really? 13:30 < obscurite> archels: i don't think anyone has made that complete a simulation before have they? it's mostly metabolism/translation but there are 26 other categories of submodel 13:30 < obscurite> archels: it seems more ambitious in that way and the visual instrumentation is really impressive 13:30 < obscurite> but i'm also fairly new to the field, so my comments in context 13:30 < obscurite> take* 13:33 < yashgaroth> it didn't surprise me to find that JCVI did it; very similar to their "synthetic bacterium" in that there's a massive effort to make something that's marginally useful, unless you count all the pop-sci speculation 13:33 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 264 seconds] 13:33 < archels> this ^ 13:33 < obscurite> yashgaroth: i belive it was only marginally related to JCVI 13:34 < obscurite> yashgaroth: mostly a stanford thing with Dr. Covert 13:34 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 13:34 < obscurite> the paper barely even mentions JCVI 13:34 < obscurite> it's just a name people know so they latch on to it :) 13:35 < yashgaroth> I'm sure JCVI's contribution was just to say "put in a massive effort for this marginal project, it worked for us" 13:35 < obscurite> oy vey. cynical commentary always enlightens me 13:35 < yashgaroth> heh sorry I try not to be cynical but I'm very rarely successful 13:36 < obscurite> at least you're aware 13:36 < obscurite> it's like "hey, where's my flying car" 13:36 < obscurite> instead of just enjoying how cool things are :) 13:36 < yashgaroth> man I am constantly enjoying how cool things are 13:36 < obscurite> good to know 13:37 < yashgaroth> in a few years a synthetic genome or a cell simulation will be useful, but doing it before then is just looking to spend money 13:38 < obscurite> i have a feeling that public funding agencies disagree 13:39 < yashgaroth> also fuck 'em for publishing in Cell, even if there isn't a more perfectly named journal for the article 13:40 < obscurite> archels: do you do compbio? 13:42 < obscurite> brb 13:43 -!- obscurite [~obscurite@danielpacker.org] has quit [Quit: leaving] 13:48 -!- obscurite [~obscurite@danielpacker.org] has joined ##hplusroadmap 13:48 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 246 seconds] 13:48 < archels> obscurite: I'm in computational neuroscience 13:51 < obscurite> archels: do you know of any attempts at whole neuron metabolic reconstruction? 13:51 < archels> metabolic? no. 13:53 < obscurite> archels: just went to a presentation on this work: http://cnl.salk.edu/Research/Synapses/ 13:54 < obscurite> old now 13:56 < archels> neat 13:56 < kanzure> is jcvi's model open source? 13:56 < obscurite> kanzure: yes, but it runs on matlab 13:56 < kanzure> or is this more proprietary crap that i will never see unless i work for them and steal their code? 13:56 < archels> obscurite: If the Cell guys had built their model entirely in MCell, I would have been impressed. :) 13:56 < obscurite> and it's not jcvi 13:57 < kanzure> obscurite: there's also things like channeldb for ion channel kinetics 13:57 < obscurite> archels: the process of creating physiologically accurate 3d models of those tiny bits was not easy from what i hear 13:58 < archels> obscurite: that's actually exactly what I am working on. :) 13:58 < obscurite> archels: do you still trace tiny silvers of tissue and stitch the splines all together? 13:59 < obscurite> kanzure: ahh cool 13:59 < kanzure> well there's software for that 13:59 < obscurite> kanzure: apparently these guys just finished making the software reasonably effecient 13:59 < obscurite> kanzure: took several years of work 14:01 < obscurite> i am guessing the advent of decent 3d printer slicing software that's open source might help new researchers 14:01 < archels> obscurite: yes, that's how neural reconstruction is done usually 14:01 < kanzure> todd/3scan has been using unpaid labor to write his image reconstruction software 14:01 < kanzure> (or so i hear) 14:02 < obscurite> any decent open source software for slice reconstruction? 14:02 < kanzure> oops s/labor/interns 14:02 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 14:02 < kanzure> i don't know any yet 14:02 < kanzure> but i think any that do exist should use something like http://github.com/kanzure/netmorph to test it with 14:04 < kanzure> e.g. by knowing the network and cell structure ahead of time you can then test that your image analyzer doesn't suck hairy nuts 14:04 < obscurite> kanzure: makes sense 14:04 < obscurite> kanzure: high res 3d printed scaffolds should also be a good test 14:05 < kanzure> jmil: what do you think? 14:06 < kanzure> jmil: here's some sample data.. 14:06 < kanzure> (18 MB) http://diyhpl.us/~bryan/papers2/neuro/nematodeuploadproject/PAG722L.png 14:06 < kanzure> jmil: does your printer have this sort of resolution? 14:06 < kanzure> i think the scale is 1 nm/pixel 14:06 < yashgaroth> hahaha 14:06 -!- splicer [~ubuntu@c83-255-190-140.bredband.comhem.se] has quit [Ping timeout: 252 seconds] 14:08 < kanzure> jrayhawk: well my bandwidth just died, oops 14:11 < kanzure> maybe i should put that on gigapan 14:11 < kanzure> or what's a non-awful site like gigapan? 14:11 -!- ThomasEgi [~thomas@gw-ko-kostr2.inka-online.net] has joined ##hplusroadmap 14:11 < obscurite> that better be one high res image 14:11 -!- ThomasEgi [~thomas@gw-ko-kostr2.inka-online.net] has quit [Changing host] 14:11 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 14:11 < yashgaroth> how close of a reproduction of that are you guys hoping to print? 14:12 < delinquentme> OK theres lots of talking going on here 14:13 < delinquentme> what are you guys doing 14:13 < kanzure> delinquentme: writing some flask/sqlalchemy crap 14:13 < delinquentme> for image recognition? 14:13 < kanzure> no 14:13 < kanzure> it's a project to mathematically model rate limits on remote servers 14:18 -!- jonnyATroot [~jonny@90.183.82.208] has joined ##hplusroadmap 14:19 -!- splicer [~ubuntu@c83-255-190-140.bredband.comhem.se] has joined ##hplusroadmap 14:19 -!- augur_ [~augur@c-75-74-129-116.hsd1.fl.comcast.net] has joined ##hplusroadmap 14:22 -!- augur [~augur@c-75-74-129-116.hsd1.fl.comcast.net] has quit [Ping timeout: 264 seconds] 14:25 -!- augur_ [~augur@c-75-74-129-116.hsd1.fl.comcast.net] has quit [Read error: Connection reset by peer] 14:27 < delinquentme> you guys heard about Theils newest investment right? 14:29 < kanzure> hi jonnyATroot 14:29 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 246 seconds] 14:31 < jonnyATroot> hi 14:31 < kanzure> jonnyATroot: what brings you here? 14:31 < jonnyATroot> biohacking 14:33 < kanzure> neat. 14:34 -!- augur [~augur@c-75-74-129-116.hsd1.fl.comcast.net] has joined ##hplusroadmap 14:44 -!- augur [~augur@c-75-74-129-116.hsd1.fl.comcast.net] has quit [Remote host closed the connection] 14:46 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 248 seconds] 14:48 < jmil> kanzure: we could print at that nematode resolution if we got a big enough grant :-D 14:49 < kanzure> i mean for individually placing neurons 14:53 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 14:56 -!- eudoxia [~eudoxia@r190-135-11-93.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 14:56 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 14:56 < eudoxia> what is the local opinion on the Open Source Ecology movement? 14:57 < eudoxia> any thoughts on how it may influence transhumanism? 14:57 < kanzure> well, fenn used to follow them more closely 14:57 < kanzure> i met marcin a few times 14:58 < kanzure> i really wish their plans and designs were better organized on the interwebs 14:59 < kanzure> someone from their clan posted to diybio a few months ago asking for some volunteers.. about something? 14:59 -!- BennyLava [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap 15:00 < kanzure> BennyLava: hi. 15:01 < kanzure> archels: did you by any chance meetup with yashgaroth when he was over a few weeks ago? 15:01 < BennyLava> hi 15:01 < yashgaroth> nope 15:03 -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Quit: jmil] 15:05 < yashgaroth> he's in eindhoven? or something 15:16 < kanzure> ever since i published my email address in a paper i have bee getting these weird spam emails about molecular biology products 15:16 < kanzure> this latest spam is from genetex 15:16 < kanzure> *been getting 15:17 < kanzure> "Dear Colleague, Through your publications, we have noted that our Rad54 antibody [C3], C-term may be useful to your current work. Rad54 is a dsDNA-dependent ATPase belonging to the Snf2/Swi2 family of SF2 helicases. It appears to have multiple roles in recombinational DNA repair. This Rad54 antibody has been validated in one western blot." 15:17 < kanzure> "We are offering our Rad54 antibody [C3], C-term to select researchers at a discounted price of $1,999 USD per 100ug vial (regularly $2,890 USD)." 15:18 -!- eudoxia [~eudoxia@r190-135-11-93.dialup.adsl.anteldata.net.uy] has quit [Ping timeout: 255 seconds] 15:18 < kanzure> i wonder if this spam works for them 15:18 < yashgaroth> I often wonder, how can all these antibody production companies stay in business? and then I remember, it's because they charge $20,000/mg 15:18 < kanzure> and patents 15:21 -!- jonnyATroot [~jonny@90.183.82.208] has quit [Quit: Leaving] 15:21 < kanzure> i wonder if anyone sells fake antibodies and gets away with it 15:23 < yashgaroth> plenty of people sell really crappy antibodies and get away with it, but there's not quiiite enough slop in biotech for an entire vial not to work 15:35 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 276 seconds] 15:35 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 15:36 -!- augur [~augur@74.174.236.67] has joined ##hplusroadmap 15:40 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 276 seconds] 15:41 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 15:47 < fenn> heh wiremound-pcr 15:47 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 15:57 < AdrianG> kanzure: do you ever get depressed 15:58 < kanzure> no 15:58 < kanzure> i mean, no i don't get clinically depressed 15:59 < kanzure> can someone look over my python/flask-sqlalchemy comments here? 15:59 < kanzure> https://github.com/mitsuhiko/flask-sqlalchemy/issues/60 15:59 < kanzure> i think i might have some inaccuracies that i'd like to edit 16:00 < delinquentme> AdrianG, depression is a focus thing methinks 16:00 < AdrianG> delinquentme: overfocusing? 16:00 < AdrianG> or underfocusing? 16:00 < delinquentme> no lack of focus 16:00 < delinquentme> yeah! 16:00 < AdrianG> uh huh 16:01 < AdrianG> very novel theory 16:01 < AdrianG> how do I proceed 16:01 < delinquentme> really?! 16:01 < kanzure> AdrianG: hah don't feed the troll 16:01 < delinquentme> I thought that was the whole idea? 16:01 < delinquentme> *MEAN FACE* 16:01 < AdrianG> :E 16:01 < kanzure> wait, no, AdrianG is the troll in this scenario 16:01 < kanzure> ermm 16:01 < kanzure> AdrianG: stop instigating him? 16:01 < AdrianG> wait wat 16:02 < AdrianG> i just got banned from reddit-depression 16:02 < AdrianG> so now i have no ouetlet 16:02 < kanzure> AdrianG: you know very well that delinquentme is incapable of reading meaning and sarcasm out of your sentences (like "very novel theory") 16:02 < delinquentme> AdrianG, reddit is dumb sometimes 16:02 < delinquentme> hahah 16:02 < kanzure> so, i think the right word is instigating 16:02 < delinquentme> AdrianG, if you're trolling me I've got coding to do 16:02 < delinquentme> but if you're being serious ill totes halp 16:03 < AdrianG> ok halp 16:03 < AdrianG> i needs all halp i can has 16:03 < AdrianG> pm 16:03 < delinquentme> sure sure 16:03 < AdrianG> im srs 16:03 < AdrianG> im on the bipolar swing right now :O 16:03 < delinquentme> sure talk to me homeslice! 16:03 < delinquentme> pm 16:03 < kanzure> wtf. 16:04 < delinquentme> ? 16:12 < fenn> AdrianG: http://george-eby-research.com/html/depression-anxiety.html and http://krispin.com/ are advice worth trying 16:12 < fenn> at the very least get yourself a little bottle of magnesium citrate 16:12 < AdrianG> i have it right in front of me 16:12 < AdrianG> a whole pile, take it erryday 16:12 < fenn> huh 16:12 < AdrianG> fenn: amphetamines are failing to keep it at bay 16:12 < AdrianG> i dont think mag citrate will save me 16:13 < AdrianG> or protein basics 16:13 < yashgaroth> this sounds like a job for lsd 16:13 < fenn> i'm sorry, since when was a nutrient the same as a drug? 16:13 < kanzure> so, i assumed you were banned from reddit because you were trolling depressed peeps 16:13 < delinquentme> yashgaroth, lol 16:14 < delinquentme> yashgaroth, well hang out sometime 16:14 < yashgaroth> also since when are amphetamines used for depression 16:15 * kanzure does some quick mental math 16:21 -!- augur_ [~augur@74.174.236.67] has joined ##hplusroadmap 16:22 < fenn> i disagree with eby's views on homeopathy and glutamate, but it doesn't detract from his message too much 16:22 < delinquentme> hio fenn 16:23 -!- augur [~augur@74.174.236.67] has quit [Read error: Connection reset by peer] 16:24 < delinquentme> anyone offhand know of a house hold substance I can feed algae with? 16:24 < delinquentme> YES yashgaroth i want to grow algae 16:24 < delinquentme> My inner flower child is blooming 16:25 < yashgaroth> do you have lights in your house? 16:25 < yashgaroth> I have no idea if they fix their own nitrogen, but light+fertilizer is probably fine oh and salt water 16:28 < delinquentme> yeah fertilizer is what was reccommended but I dont have any laying around? 16:28 < delinquentme> and really just like that mulch stuff? 16:28 < delinquentme> OOO wait I do have a compost pile... 16:28 < delinquentme> lol god 16:28 * delinquentme has said too much 16:28 < delinquentme> brothers / sisters preez no judge me. 16:28 -!- augur_ [~augur@74.174.236.67] has quit [Remote host closed the connection] 16:30 < kanzure> yashgaroth: usually you want to cycle carbon dioxide through the tanks if you want to optimize their growth logs 16:30 < yashgaroth> oh well if you're gonna be fancy about it 16:30 < kanzure> miracle growth works pretty well especially with that nitrogen conte-- 16:30 < kanzure> yes why would you want to be fancy :( 16:31 < yashgaroth> damn straight, but yeah just don't go and buy a 50-lb bag of ammonium nitrate and you'll be fine 16:32 < kanzure> tell them it's for "rocket experiments" 16:32 < kanzure> also be muslim when doing that 16:32 < delinquentme> lolol 16:32 < delinquentme> kanzure, so when im logging in via a form 16:33 < delinquentme> I need to store cookie information on whatever program I'm using to automate the login with? 16:33 < kanzure> yes 16:33 < delinquentme> is this a form of mutual authentication? 16:33 < kanzure> no 16:33 < kanzure> i highly recommend http://python-requests.org/ btw 16:34 < delinquentme> So whatever is in the cookie is the chrome profiler needs to be written into a mechanize cookie? 16:35 < kanzure> well yes, but why not just write code to do the login and save the cookie too? 16:35 < kanzure> the cookie will expire on the remote server eventually 16:36 < kanzure> so it's better to just add the login step to the scraper 16:37 < delinquentme> yeahh I've got the login step 16:37 < delinquentme> its just that after submission Im getting no feedback that the request was sucessful 16:37 < kanzure> it might be a redirect 16:37 < delinquentme> ( i've verified the form fields as filled and correct ) 16:37 < kanzure> via the Location header 16:38 < delinquentme> yeah but mechanize usually handles those 16:38 < delinquentme> i can check redirects in chrome right 16:38 < delinquentme> ahh ok im geting a 302 16:44 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 248 seconds] 16:47 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap 16:54 -!- Steel2 [ad42e490@gateway/web/freenode/ip.173.66.228.144] has joined ##hplusroadmap 17:25 -!- Thorbinator1 [~Thorbinat@c-67-166-146-169.hsd1.ca.comcast.net] has quit [Read error: Connection reset by peer] 17:26 -!- Thorbinator [~Thorbinat@c-67-166-146-169.hsd1.ca.comcast.net] has joined ##hplusroadmap 17:34 < delinquentme> OOGA-WOOGA-BOOGA 17:34 < delinquentme> =p 17:34 < BennyLava> my loony bun is fine 17:36 < delinquentme> ^^ 17:39 < kanzure> delinquentme: btw you should also consider pyphantomjs http://github.com/kanzure/pyphantomjs 17:39 < kanzure> especially since it's.. python. 18:04 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 18:11 -!- rafael [~dnm@pdpc/supporter/active/calango] has quit [Quit: Leaving] 18:19 -!- rmcl [~rmcl@rrcs-108-178-168-32.west.biz.rr.com] has quit [Quit: rmcl] 18:28 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 18:29 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 18:39 -!- nmz787 [~Nathan@107.55.171.21] has joined ##hplusroadmap 18:43 -!- rmcl [~rmcl@ip-64-134-60-60.public.wayport.net] has joined ##hplusroadmap 18:43 < kanzure> neat, the fbi paid me today 18:44 < yashgaroth> man that undercover work is gonna get you killed one day 18:45 < kanzure> nah this was just their meal coverage 18:45 < yashgaroth> oh, right 18:45 < kanzure> $316.50 i think is just the minimum they were paying out 18:45 < kanzure> i'm sure nmz787 asked for >$500 with all that gas money.. 18:45 < kanzure> diesel money, i mean. 18:46 < yashgaroth> has he converted it to biodiesel yet? 18:46 < kanzure> nmz787: ping? 18:47 < yashgaroth> I suppose it's only recommended if he does move to the best coast, it'd probably solidify in the frozen northlands of NY 18:54 -!- rmcl [~rmcl@ip-64-134-60-60.public.wayport.net] has quit [Quit: rmcl] 19:15 -!- Thorbinator [~Thorbinat@c-67-166-146-169.hsd1.ca.comcast.net] has quit [Read error: Connection reset by peer] 19:15 -!- Thorbinator [~Thorbinat@c-67-166-146-169.hsd1.ca.comcast.net] has joined ##hplusroadmap 19:33 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 246 seconds] 19:35 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 19:50 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 19:51 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has joined ##hplusroadmap 19:59 -!- nmz787 [~Nathan@107.55.171.21] has quit [Ping timeout: 276 seconds] 20:22 -!- klafka1 [~textual@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 20:25 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Ping timeout: 250 seconds] 20:30 < kanzure> all this coursera/udacity/courseware stuff is nice in theory but in practice there's only ever "intro to x" videos, which don't really teach you anything 20:31 < kanzure> where's my "5th year phenomenenology" videos 20:31 < kanzure> *phenomenonology 20:33 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 20:34 -!- klafka_ [~textual@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap 20:34 -!- klafka1 [~textual@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Ping timeout: 255 seconds] 20:37 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Ping timeout: 244 seconds] 20:39 < kanzure> hmm so mac uses reddit, how disappointing 20:39 < kanzure> http://www.reddit.com/r/hwstartups/comments/x3xjc/whitebox_blog_another_open_pcr_project_16_wells/ 20:44 < klafka_> i thought reddit was banned here kanzure 20:44 < klafka_> :P 20:45 < yashgaroth> to be fair he's not posting atheist rage comics or anything 20:46 -!- WashIrving [~WashIrvin@110.92.96.113] has joined ##hplusroadmap 20:46 < kanzure> klafka_: i think tastefully making fun of friends for using reddit is totally ok 20:46 < klafka_> :P 20:46 < klafka_> JK 20:46 < kanzure> but, you're right 20:59 -!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [] 20:59 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap 21:02 < kanzure> i guess i should get delinquentme and _sol_ to meetup sometme 21:02 < delinquentme> HMMm he /she is not in le channal? 21:03 < kanzure> well, maybe you're the same person. 21:03 < kanzure> but i sort of doubt you have two residential ip addresses by the same provider 21:03 < klafka_> lol 21:06 < delinquentme> O_o;;; 21:07 < delinquentme> i KNEW that TSA scan was a little too personal! 21:07 < delinquentme> ... if that wasn't funny to anyone, I'm sorry you missed out bc it was hilarious in my head. 21:17 -!- WashIrving [~WashIrvin@110.92.96.113] has quit [Read error: Connection reset by peer] 21:18 -!- gedankenstuecke [~bastian@phylomemetic-tree.de] has quit [Ping timeout: 272 seconds] 21:18 -!- gedankenstuecke [~bastian@phylomemetic-tree.de] has joined ##hplusroadmap 21:22 -!- WashIrving [~WashIrvin@110.92.96.113] has joined ##hplusroadmap 21:30 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Quit: leaving] 21:33 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has joined ##hplusroadmap 21:51 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Quit: rmcl] 21:52 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has quit [Quit: Leaving] 21:54 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Quit: Leaving] 22:05 -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has quit [Quit: jmil] 22:06 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:09 -!- Thorbinator [~Thorbinat@c-67-166-146-169.hsd1.ca.comcast.net] has quit [Read error: Connection reset by peer] 22:13 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:13 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:16 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:16 -!- rmcl_ [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:19 -!- srangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap 22:21 -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 264 seconds] 22:21 -!- srangewarp is now known as strangewarp 22:23 -!- rmcl_ [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:24 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:28 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:29 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:31 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:31 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:36 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:36 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:37 < kanzure> daeken's been in the news for his onity hack: http://www.bbc.co.uk/news/technology-18968225 22:39 < kanzure> http://demoseen.com/bhtalk2.pdf 22:39 < kanzure> http://demoseen.com/bhpaper.html 22:39 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Read error: Connection reset by peer] 22:39 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:40 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Client Quit] 22:43 -!- BennyLava [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has quit [Killed (kloeri_ (Spam is not allowed on freenode.))] 22:45 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 22:55 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Quit: Leaving] 23:12 -!- augur [~augur@208.58.5.87] has quit [Read error: Connection reset by peer] 23:12 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 23:50 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap --- Log closed Wed Jul 25 00:00:18 2012