--- Log opened Fri Aug 03 00:00:27 2012 00:01 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 00:13 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Quit: Leaving] 00:27 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 01:46 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 248 seconds] 01:57 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 02:01 -!- AdrianG_ [~ircname@unaffiliated/amphetamine] has joined ##hplusroadmap 02:04 -!- AdrienG [~ircname@unaffiliated/amphetamine] has quit [Ping timeout: 255 seconds] 02:12 -!- nsh- [~Smedley@host86-165-113-6.range86-165.btcentralplus.com] has joined ##hplusroadmap 02:14 -!- nsh [~Smedley@wikipedia/nsh] has quit [Ping timeout: 240 seconds] 02:22 -!- nsh- is now known as nsh 02:22 -!- nsh [~Smedley@host86-165-113-6.range86-165.btcentralplus.com] has quit [Changing host] 02:22 -!- nsh [~Smedley@wikipedia/nsh] has joined ##hplusroadmap 02:36 -!- sylph_mako [~mako@118-92-250-50.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 255 seconds] 03:52 -!- SolG [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap 03:53 -!- fenn_ [~fenn@131.252.130.248] has joined ##hplusroadmap 03:53 -!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 244 seconds] 03:53 -!- Steel2 [ad42e490@gateway/web/freenode/ip.173.66.228.144] has quit [Ping timeout: 245 seconds] 03:53 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has quit [Ping timeout: 244 seconds] 03:53 -!- fenn [~fenn@131.252.130.248] has quit [Ping timeout: 240 seconds] 03:53 -!- Charlie [~quassel@64.31.59.70] has joined ##hplusroadmap 03:53 -!- augur_ [~augur@208.58.5.87] has joined ##hplusroadmap 03:53 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has joined ##hplusroadmap 03:53 -!- Charlie_ [~quassel@64.31.59.70] has quit [Ping timeout: 240 seconds] 03:53 -!- Charlie is now known as Guest23590 03:54 -!- augur [~augur@208.58.5.87] has quit [Ping timeout: 244 seconds] 04:36 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Read error: Connection reset by peer] 04:38 -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap 04:50 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 04:51 -!- nsh [~Smedley@wikipedia/nsh] has quit [Remote host closed the connection] 04:55 -!- hplunny [~hplunny@host86-165-113-6.range86-165.btcentralplus.com] has quit [Ping timeout: 240 seconds] 05:09 < archels> superkuh: I just launched wget -r on your library, if your nets just slowed to a crawl, feel free to tell me to fuck off. 05:29 -!- exiff [~ficl@unaffiliated/exiff] has quit [Ping timeout: 248 seconds] 05:44 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 240 seconds] 05:57 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 06:03 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap 06:11 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 248 seconds] 06:19 < delinquentme> <4 06:26 -!- skorket [~skorket@cpe-24-58-232-122.twcny.res.rr.com] has quit [Quit: Leaving] 06:29 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 06:34 < archels> superkuh: okay, done 06:34 < archels> Downloaded: 531 files, 4.3G in 1h 9m 53s (1.05 MB/s) 06:34 < archels> <3 06:48 < delinquentme> <2! 06:58 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 248 seconds] 07:00 -!- SolG is now known as _sol_ 07:00 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 07:01 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 07:08 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 08:02 < delinquentme> where do people sell domains? 08:07 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Ping timeout: 276 seconds] 08:18 -!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has joined ##hplusroadmap 08:18 -!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has quit [Changing host] 08:18 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 08:20 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 08:22 -!- nathaniel [~nathaniel@reddit/operator/nathaniel] has quit [Quit: Leaving] 09:10 -!- exiff [~ficl@unaffiliated/exiff] has joined ##hplusroadmap 09:18 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 245 seconds] 09:23 -!- ianmathwiz7 [~chatzilla@64-91-71-48.stat.centurytel.net] has joined ##hplusroadmap 09:25 < kanzure> delinquentme: flippa 09:26 < kanzure> "I am a Bio major at CSULA and I am very excited that I have found this group. I am interested in your projects and I would love to participate and help in any way possible." 09:26 < kanzure> ". I am currently interested in all fields of Bio ( or any other science) but I especially like genetic manipulation or any biotechnology. I hope to learn more advanced lab techniques/procedures as well as the knowledge and theory that i is required to perform such experiments." 09:29 < kanzure> "I want to do a simple little experiment to show to the nephews. I'd like to take some E. coli and make them glow by some good ol' fashioned bioluminescence using the pLux gene from Vibrio fischeri. I've done similar recombinant stuff in the lab at school and it's a super straight forward procedure so I thought it'd be fun." 09:29 < kanzure> "Trouble is... Where do I get the plasmids? I cannot find plasmids for the pLux gene anywhere. I thought about ordering a culture of V. fischeri and extracting the gene out via PCR... but I do not have a PCR machine yet. Hell, I don't even know if I can make a plasmid out of PCR products??" 09:30 < kanzure> "But how can I combine that, remove the bad plasmide from that bacterium and insert the glowing genes (but not the saline genes, because the tree shouldn't need salty water to live like the bacterium)." 09:31 < kanzure> "I read about DIYbio in Discover Magazine, and I was instantly in love with the idea of DIY organisms. However, I don't have anything beyond a very basic layman's understanding of how DNA functions..." 09:31 < kanzure> "Where should a noob like me start looking for information? The idea I had was to introduce a gene into a cactus to change it's color, or even make it glow if possible." 09:32 < kanzure> "I'm from the DC area looking to start a project up. I could use some insight and advice on how to get my project going, and if anybody is in the area, people who might be interested hearing about it and collaborating. While I'm still developing my idea, I envision the project sort of iGEM-esque (or maybe that's how all these projects start...)," 09:32 < kanzure> "but would like some input as to how people start their projects; if no formal laboratory facilities are availabe, how/where people purchase lab equipment; and maybe some info on unforseen experimental challenges or technical difficulties people have had." 09:32 < kanzure> "...trying to make myself cognizant of these issues as I develop my project. Thanks for any and all advice in advance." 09:33 < kanzure> "1) Is it worthwhile to take the "reverse genetics" approach, inserting and removing genes with the hope of a meaningful outcome?" 09:33 < kanzure> "2.) I was looking at beginning my research with the Drosophilas and microinjection. Is this a decent starting point for a low-budget? I've built my own functional micromanipulator, but I'm not familiar with pricing for gene synthesis etc." 09:34 < kanzure> "Can anyone help me get into genetic engineering. What can I do? How can I start? The thing I am looking to do with it is (with no aims or goals like to cure cancer) create a living creature, or create biological circuits so that you have living electronics (hack my toys to make them pets!)." 09:34 < kanzure> "What could I do to do a "hello world?"" 09:35 < kanzure> "I studied mathematic, and i'm currently studying cognitive science in college. What you people are doing seem pretty interesting. I've always been interested by science. So my question would be naive : where do i start?" 09:38 < kanzure> "I'm getting really interested in DIYBio and hope to do stuff with chlorophyll (green, plant stuff, sorry if that was the wrong word!) and bacteria. Unfortunatly, I have no idea how to get dna into bacteria. I think it involves getting plasmids but I don't know much. Any help at all would be great!!" 09:41 < kanzure> oh right i forgot about http://www.labautopedia.com/mw/index.php/Main_Page 09:43 < kanzure> http://www.labautopedia.com/mw/index.php/Category:Automated_liquid_handling_workstations 09:43 < kanzure> http://www.labautopedia.com/mw/index.php/The_history_of_automated_liquid_handling 09:45 < kanzure> http://www.labautopedia.com/mw/index.php/Early_microplate_automation 09:46 < kanzure> "The procedure was to first add diluent, to all 96 wells with a dropper. The technician holding eight loops at a time would move them from row to row, twirling to obtain mixing, and then transfer 25μL or 50μL to the next row (Figure 3)" 09:52 -!- rmcl [~rmcl@rrcs-108-178-168-32.west.biz.rr.com] has joined ##hplusroadmap 09:56 < kanzure> oh man, old advertising http://aem.asm.org/content/30/1/local/advertising.pdf 09:58 < kanzure> "The accepted method of cleaning the Microtiter® loop was to flame them to incandescence, then dip them in water. The sudden contraction popped off contaminates. To accomplish this, the Autotiter had a propane burner and a running water bath. At an ASM meeting in Philadelphia someone reversed the connections." 09:58 < kanzure> "The effort to light the burner resulted in a propane-fed flaming torch about three feet high. With the stench of singed hair still prevalent, the Fire Marshall arrived on the scene to remind us open flames were not allowed." 09:59 < octopine> kanzure hmm? http://d.pr/i/IyE8+#.png 09:59 < kanzure> "Morning Star was a large injection-molding house producing eight track cassettes. They and Dynatech (Cooke) created a joint venture to produce microplates in high volume to meet the growing demand. The new company was named CoStar." 10:05 < kanzure> "history of the pipette" http://www.youtube.com/watch?v=IRVFZJ-VLCc 10:13 -!- augur_ is now known as augur 10:40 -!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 244 seconds] 10:49 -!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap 10:52 -!- ianmathwiz7 [~chatzilla@64-91-71-48.stat.centurytel.net] has quit [Quit: ChatZilla 0.9.88.2 [SeaMonkey 2.11/20120715041107]] 11:04 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 11:15 -!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap 11:17 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Ping timeout: 276 seconds] 11:19 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap 11:26 -!- Netsplit *.net <-> *.split quits: chido, ThomasEgi, archels, strages_home, strangewarp, OldCoder_ 11:26 -!- Netsplit over, joins: archels, ThomasEgi, OldCoder_, strangewarp, strages_home, chido 11:32 -!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap 11:34 -!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Ping timeout: 245 seconds] 11:36 -!- Netsplit *.net <-> *.split quits: Sanky, CIA-10, Vicarious, HEx1, obscurite, jennicde_, azonenberg, Lemminkainen_, phryk, epitron, (+23 more, use /NETSPLIT to show all of them) 11:37 -!- Netsplit over, joins: exiff, qnm, fenn_, _sol_, Mokbortolan_1, superkuh, Lemminkainen_, phryk, drazak, Jenda` (+21 more) 11:43 < nmz7871> kanzure: labautopedia isnt working, are you harassing their servers? 11:43 -!- nmz7871 is now known as nmz787 11:49 < kanzure> no 11:49 < kanzure> i have copies of those two history pages though 11:51 -!- Coornail [~Coornail@li66-97.members.linode.com] has joined ##hplusroadmap 11:51 -!- HEx1 [~HEx@212.159.112.196] has joined ##hplusroadmap 11:52 -!- sylph_mako [~mako@118-92-250-50.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap 12:00 -!- sylph_mako [~mako@118-92-250-50.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 244 seconds] 12:16 -!- sylph_mako [~mako@118-92-250-50.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap 12:18 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 12:19 -!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 248 seconds] 12:45 -!- AdrianG_ is now known as AdrienG 13:09 < nmz787> 'linuz torvalds - nvidia fuck you' http://www.youtube.com/watch?v=iYWzMvlj2RQ 13:10 < kanzure> http://www.3ders.org/articles/20120803-first-open-source-powder-based-3d-printer.html 13:10 < kanzure> http://blog.makezine.com/2012/08/03/oshw-powder-based-rapid-prototyper/ 13:12 < nmz787> 96 DPI!!! 13:12 < nmz787> geez 13:15 < kanzure> nmz787: http://diyhpl.us/~bryan/irc/labautopedia-history-of-early-microplate-automation.html 13:15 < kanzure> nmz787: http://diyhpl.us/~bryan/irc/labautopedia-history-of-automated-liquid-handling.html 13:59 < nmz787> kanzure: you gonna get the IMGs? 13:59 < kanzure> i have the images, but i didn't upload them 14:05 < AdrienG> kanzure: http://www.spoonflower.com/welcome 14:15 < kanzure> oh i forgot about chris' "personalpcr" thing.. http://templemanautomation.com/personalpcr/ 14:22 < nmz787> man i can't even find a decent google hit for 'new york city overubanization' 14:22 < nmz787> where does a guy go to hate on NYC??? 14:27 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Read error: Connection reset by peer] 14:28 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 14:28 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Ping timeout: 248 seconds] 14:29 < kanzure> http://openwetware.org/wiki/TOP10_chemically_competent_cells 14:31 -!- Steel_ [ad42e490@gateway/web/freenode/ip.173.66.228.144] has joined ##hplusroadmap 14:35 -!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has joined ##hplusroadmap 14:35 -!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has quit [Changing host] 14:35 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap 14:42 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Ping timeout: 264 seconds] 14:50 < kanzure> nmz787: what happened to http://diybioforum.org/wiki/index.php?title=FAQ 14:58 < nmz787> no one ever really used it 14:59 < nmz787> i don't think there was much of anything there anyway... not sure if i backed it up 15:07 < gnusha> diyhpluswiki.git: 21c5bf6 an initial list of better questions for a biohacking faq 15:07 < gnusha> diyhpluswiki.git: 5be2a4a 2012-07-05 Never mind the biologists, here come the biopunks 15:07 < gnusha> diyhpluswiki.git: a004f61 add link to openwetware registration discussion 15:07 < gnusha> diyhpluswiki.git: 22039e0 even more questions in diybio/faq/better-questions 15:07 < kanzure> nmz787: http://diyhpl.us/wiki/diybio/faq/better-questions 15:07 < kanzure> please add anything you think is missing 15:07 < kanzure> these were extracted from the mailing list archives 15:10 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 272 seconds] 15:11 -!- nathaniel [~nathaniel@reddit/operator/nathaniel] has joined ##hplusroadmap 15:13 < gnusha> diyhpluswiki.git: fee376b fix sterilization typo 15:14 < kanzure> the faq should probably be segmented into different sections 15:14 < kanzure> there's usually a big set of questions even for each individual protocol 15:15 < kanzure> e.g. always someone wondering about modifying the calcium chloride transformation protocols 15:15 < kanzure> or the inevitable questions about why their gel runs are smearing 15:15 < nmz787> gels are something of a black art i think 15:15 < nmz787> or maybe the people in the know only publish answers in springer books 15:16 < nmz787> that i haven't found and downloaded yet 15:16 < kanzure> the most probable reason that your gel smears is that god hates you 15:16 < kanzure> the second most probable reason is contamination 15:18 < nmz787> hah 15:18 < nmz787> not contam 15:18 < nmz787> no really weird shit can happen 15:19 < kanzure> well, i had contamination issues when i spent a month running 100s of gels 15:19 < kanzure> to be fair i was doing weird things 15:19 < kanzure> e.g. the problems were mostly upstream in the pcr steps 15:19 < nmz787> tom knight was telling me to do extra DNA precipitations a while ago, that helped things (getting rid of other ions) but it didn't make them perfect 15:19 < nmz787> not just smearing though, wiggly lanes and weird shit 15:20 < nmz787> like someone took a picture of a perfect gel, printed it on transparency plastic, then warmed up the plastic so it got all deformed 15:20 < kanzure> "the weegie board guide to molecular biology (not endorsed by the national spiritualist organization)" 15:21 < nmz787> the new FAQ questions look good 15:21 < kanzure> they should probably be split up 15:21 < kanzure> into segments 15:21 < nmz787> we could have been using shitty agarose, though I don't know 15:22 < nmz787> hah, i just noticed one of my own questions 15:23 < kanzure> the sections could be: gel electrophoresis questions, transformation questions, incubation, all things glowing/gfp, thermocycling/pcr, dna extraction, sequencing, primers and primer design, stochiometry/dilutions/buffers/math, making equipment, buying equipment 15:23 < kanzure> but this is already way too many subtopics for one person to deal with 15:23 < nmz787> "Are there any poly(T) oligo columns on sale that have a free 3' end" 15:24 < kanzure> there are some other questions of yours in there 15:24 < nmz787> a bit too technical for a beginner, cory was the only one to reply :P 15:24 < kanzure> oh and then there's lots of software questions, which should be organized somehow 15:24 < nmz787> there could be a question "Do only PhDs answer these FAQ questions" 15:26 < nmz787> geez another one from me... i must be the redheaded stepchild of DIYbio that doesn't get noticed because i'm shoved in the closet under the stairs 15:26 < kanzure> i don't understand that analogy at all 15:26 < nmz787> lol 15:26 < nmz787> you could copy the P is for plasmid google doc that was floating around for the glossary 15:27 < nmz787> 'a is for autoclave' https://docs.google.com/document/d/10OlwuiGhIIToPlzpywQhOL0hNyAWdZsR06DPubjlcrM/edit 15:29 < gnusha> diyhpluswiki.git: 5b172a4 possible way to organize these questions 15:30 < nmz787> i've been in the habit of capitalizing Science lately 15:30 < nmz787> like the religious folks do with their deities 15:32 < kanzure> organizing questions around different methods (gels, blots, precipitation, ...) feels like it's missing the ponit 15:32 < kanzure> *point 15:32 < kanzure> but they are still important questions 15:32 < kanzure> like, i can't manually maintain a FAQ for all 4000 protocols on protocols-online.org.. if each protocol gets 10 questions that's 40,000 questions. 15:36 < nmz787> yeah 15:36 < nmz787> i was thinking a lot of the questions are great, and worth taking the time to add answers 15:36 < nmz787> but that it would be hard for someone to search through 15:36 < nmz787> since there are so many 15:37 < kanzure> i have noticed that many people read about diybio in the news 15:37 < kanzure> and then join the mailing list 15:37 < kanzure> and then ask "okay, what do i need to get started" 15:37 < kanzure> which is a totally hopeless question.. 15:38 < kanzure> there are just so many possible projects that it's difficult to enumerate them all 15:40 < nmz787> yeah, well, if they need somewhere to get started... we either put them through the PITA many roads..... or we say Carolina Kit 15:40 < nmz787> but even that doesn't give you gel loading or analysis training 15:40 < kanzure> why is everyone so interested in gfp? i did the gfp kit in high school and it wasn't that exciting. 15:41 < nmz787> HOWTO use a micropipettor can be a challenge for college students 15:41 < kanzure> gfp is very useful in research projects for other reasons, but by that point you are using it for some practical ends 15:41 < nmz787> I've seen students use them without tips, and heard stories of grad students doing that too 15:41 < nmz787> then again i started biohacking in my kitchen after 9th grade 15:42 < nmz787> but i started with books and long ass FAQs 15:42 < nmz787> which I was fine with 15:42 < gnusha> diyhpluswiki.git: 112166f 9 more questions 15:44 < gnusha> diyhpluswiki.git: e45fbc9 just some questions about open access 15:45 -!- skorket [~skorket@cpe-24-58-232-122.twcny.res.rr.com] has joined ##hplusroadmap 15:46 < skorket> have you guys heard of/used OMERO? 15:46 < kanzure> no 15:46 < nmz787> nope 15:46 < kanzure> it sounds like an ftp server for microscopy images 15:46 < skorket> more like a filesystem/management environment 15:50 < kanzure> huh, maradydd was making her own calcium chloride? 15:51 < kanzure> skorket: you have been pre-selected; we are redoing the diybio faq 15:51 < kanzure> skorket: what questions would you like to see answered? 15:51 < skorket> I won't be representative 15:51 < kanzure> i know 15:51 < kanzure> go ahead anyway :P 15:51 < skorket> what kind of questions do you want? 15:53 < kanzure> skorket: any kind 15:53 < kanzure> i know you had some basic microfluidic questions, but questions simpler than that would be cool 15:54 < kanzure> or related. 15:54 < kanzure> skorket: http://diyhpl.us/wiki/diybio/faq/better-questions 15:54 < skorket> what are some good resources for biology, diy and diy biology? 15:54 < gnusha> diyhpluswiki.git: 6f859d5 more questions from the diybio mailing list 15:56 < skorket> who should I go through to get reagents? Will I run into problems as an individual ordering reagents? 15:57 < skorket> yeah, see, you have most of the questions I would ask on that list in one form or another 16:03 < gnusha> diyhpluswiki.git: a12623a a handful of questions imported from the existing faq 16:13 -!- exiff [~ficl@unaffiliated/exiff] has quit [Ping timeout: 255 seconds] 16:20 -!- SDr|SF is now known as SDr|Denver 16:32 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] 16:33 -!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap 16:36 -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] 16:47 < kanzure> ybit: happy birthday, old man 17:38 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Quit: Leaving] 17:56 < kanzure> when will "like a roomba stuck in a closet" become an acceptable punchline? 17:57 < kanzure> i fear that roombas have already reached peak popularity 17:57 < kanzure> so the joke might not be that strong 17:58 < nmz787> punchline to what though? 17:58 < kanzure> *shrug* 17:59 * kanzure protects his list of zingers (ok, i don't have a list of zingers really) 18:06 < nmz787> mmm hmm 18:06 < nmz787> more like your list of potential punchlines 18:07 -!- rmcl [~rmcl@rrcs-108-178-168-32.west.biz.rr.com] has quit [Quit: rmcl] 18:35 < gnusha> diyhpluswiki.git: 69473a5 rearranging some questions, adding others 18:43 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 18:49 < brownies> kanzure: i found a bug 18:49 < brownies> clicking on a question does not seem to reveal an answer -_- 18:51 < kanzure> heh 18:55 < kanzure> brownies: there are answers here http://diyhpl.us/wiki/diybio/faq for some of them 18:57 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap 18:58 < kanzure> delinquentme: https://www.transcriptic.com/about/jobs 18:58 < kanzure> thought you might like that one. 18:59 < delinquentme> OOOooOOooOOoO 18:59 * delinquentme munches quesidilla 19:19 < kanzure> "illustrated guide to home biology experiments" http://shop.oreilly.com/product/0636920017691.do 19:21 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 19:24 < gnusha> diyhpluswiki.git: 3e56974 just some introductory books 19:25 < kanzure> yashgaroth: got any better ideas? http://diyhpl.us/wiki/diybio/faq/better-questions 19:26 < yashgaroth> you did all that since yesterday? 19:27 < yashgaroth> "Why are there no really good biology forums?" oh god it's so true :( 19:30 < yashgaroth> looks good to me, should fill in the answers before adding new Qs 19:31 < kanzure> yeah i don't know how to structure it, a lot of these aren't actually "frequently" asked 19:31 < yashgaroth> anyone know if the khan academy videos for bio are any good? might as well add a link if they are 19:32 < kanzure> i haven't tried them yet 19:32 < yashgaroth> oh and NCBI bookshelf would be good 19:33 < kanzure> that's mentioned in the actual faq elsewhere 19:33 < kanzure> so, these are just questions that i extracted from the mailing list 19:34 < kanzure> the number one most frequently asked question is a meaningless one ("how do i get started") 19:36 < yashgaroth> damn they're coming out of the woodwork in that non-biology-native guy's thread 19:38 < kanzure> "Is there a simple kit to order and start with or a book that isn't a textbook?" 19:38 < kanzure> that isn't a textbook? what does he want, biology by fortune cookie notes? 19:39 < yashgaroth> honestly neither the google group nor diybio.org actually have biology information or links to it, far as I can tell 19:39 < kanzure> that's true 19:39 < kanzure> and no link to the faq 19:40 < kanzure> i have been debating whether or not i should send out an email about the faq being moved 19:40 < yashgaroth> who owns the group, mac cowell? 19:40 < kanzure> i didn't ask jonathan cline about moving it 19:40 < kanzure> mac cowell and jason bobe 19:41 < yashgaroth> yeah a link to OWW and/or the faq would theoretically cut down on the getting started spam...theoretically 19:42 < kanzure> i don't think the oww faq does a good job 19:42 < yashgaroth> well it's better than "welcome to the group! here's a link to a worthless site! ask stupid questions below" 19:43 < kanzure> haha 19:43 < kanzure> well, i think i should send out an email about the faq move 19:43 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Quit: rmcl] 19:43 < kanzure> but, moving the faq probably makes me an ass 19:43 < yashgaroth> it's not like they're doing anything with it 19:44 < kanzure> true that 19:47 < gnusha> diyhpluswiki.git: 417c5f6 update internal wiki links in diybio/faq.mdwn 19:48 < gnusha> diyhpluswiki.git: 0ca32ef hmm that diybio.org/projects link looks deprecated 19:49 -!- klafka1 [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 19:51 < gnusha> diyhpluswiki.git: b6f158b fix some faq interlinking 19:52 -!- rmcl [~rmcl@99.71.136.35] has joined ##hplusroadmap 19:52 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Ping timeout: 240 seconds] 19:58 -!- rmcl [~rmcl@99.71.136.35] has quit [Quit: rmcl] 20:00 < kanzure> here is fenn complaining about openwetware registration policies: 20:00 < kanzure> https://groups.google.com/forum/?fromgroups#!topic/diybio/50U54BYu9Rw 20:01 < kanzure> here is mac cowell hating on ikiwiki: 20:01 < kanzure> https://groups.google.com/forum/?fromgroups#!searchin/diybio/FAQ/diybio/Whbc4_yQF4s/BZK0-0BaRU8J 20:01 < kanzure> oops i mean 20:01 < kanzure> https://groups.google.com/forum/?fromgroups#!searchin/diybio/FAQ/diybio/Whbc4_yQF4s/BZK0-0BaRU8J 20:01 < kanzure> hrm. 20:01 < kanzure> https://groups.google.com/d/msg/diybio/Whbc4_yQF4s/FY6bxmlcI3kJ 20:01 -!- klafka1 [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has quit [Read error: Operation timed out] 20:05 -!- joshcryer [g@unaffiliated/joshcryer] has joined ##hplusroadmap 20:06 < delinquentme> http://imgur.com/gallery/44cYg 20:06 < delinquentme> " Imagine how big that is " 20:07 < joshcryer> Expected porn. Was acceptable. 20:08 < superkuh> I remember that event. 20:08 < delinquentme> lolol 20:09 < delinquentme> so 1.3 m earths would fit inside the sun... 20:09 < delinquentme> so yeah that flame is like 20:09 < delinquentme> plasma ? 20:09 < superkuh> That comment thread is painful. 20:09 < delinquentme> substantially larger that this planet 20:10 < superkuh> The earth would be the size of one of the small bright dots on the photosphere. Probably 20 could fit across the circumference of that loop. 20:11 < superkuh> Er diameter. 20:11 < superkuh> Not circumference. 20:12 < superkuh> Anyway, the image itself is in 30.4nm light from helium ions in the plasma. 20:16 < joshcryer> I recall a really convoluted video presentation about the magnetic field lines in the core of the sun. 20:16 < joshcryer> Since apparently the surface moves at rates that are counterintuitive or something. 20:16 < joshcryer> Been awhile since I saw it. 20:16 < joshcryer> Some NOVA doco on the sun. 20:16 < joshcryer> Those big outbursts are caused by magnetic loops and twists that "snap." 20:18 -!- klafka [~textual@c-67-174-253-229.hsd1.ca.comcast.net] has joined ##hplusroadmap 20:19 < nmz787> sooo, downconvert the ultra UV to vis and have some kinda pinhole camera focused on your wall during the day... tracking the sun 20:19 < superkuh> No. The atmosphere absorbs it. 20:19 < nmz787> seeing the surface of the sun for a wall would be pretty cool 20:20 < nmz787> oh 20:20 < nmz787> lame 20:20 * nmz787 goes to get some CFCs 20:21 < superkuh> For earth bound observations of the photosphere the hydrogen alpha line is best. Or if you care about photospheric magnetic fields then the iron I infrared line at ~1564 nm. 20:29 < kanzure> jrayhawk: how confident are you that pinyshell is safe? 20:36 -!- AdrienG [~ircname@unaffiliated/amphetamine] has quit [Ping timeout: 245 seconds] 20:45 < kanzure> nmz787: can you review this email? http://diyhpl.us/~bryan/irc/faq-email.txt 20:45 < kanzure> nmz787: i want to avoid pissing people off 20:46 < brownies> any email longer than 3 sentences is guaranteed to piss someone off 20:46 < nmz787> add cathal to thx list 20:47 < kanzure> nmz787: cathal didn't edit it 20:47 < kanzure> ever.. 20:47 < kanzure> brownies: well, it's a complicated history going on here 20:47 < kanzure> brownies: i wrote this retarded and embarrassing diybio faq on heybryan.org/mediawiki 20:48 < kanzure> brownies: then mac copied it to openwetware.org 20:48 < kanzure> and it's been there for a few years, where jonathan cline has been updating it with me 20:48 < kanzure> so, in theory, there's no problem with me moving it off of openwetware 20:48 < kanzure> (there are some issues with openwetware like: you have to register and wait for approval to edit) 20:49 < kanzure> brownies: so there will possibly be some people angry with the fact that i have forked it 20:49 < brownies> i was objecting generally to the wall of text, but, yes, now i see there are several other things that will also probably piss someone off 20:50 < kanzure> it's sorta stupid; jonathan is the only person who has edited it more than i have 20:51 < kanzure> and jonathan probably doesn't give a fuck 20:52 < nmz787> remove this "with the exception of Nathan McCorkle's diybioforums.org version" 20:52 < kanzure> bah 20:52 < kanzure> fine 20:52 < kanzure> i am pretty sure there are some edits there that i am forgetting :) 20:52 < nmz787> its pretty tl;dr 20:53 < kanzure> well the only thing people should care about is the first link 20:53 < nmz787> tho i did read it 20:54 < nmz787> "There has also been a trickle of other contributors to the FAQ including:" 20:54 < nmz787> you're saying Cathal didn't ever help with FAQ stuff 20:54 < kanzure> you can track that by typing: git log --follow diybio/faq.mdwn 20:54 < kanzure> yes 20:54 < nmz787> what about the new stuff 20:55 < nmz787> oh 20:55 < nmz787> ok 20:55 < kanzure> git log --follow diybio/faq.mdwn | grep "^Author: " | sort | uniq 20:56 < nmz787> yeah I think it's pretty fine 21:00 -!- wizardro [~usorid@76.23.254.105] has joined ##hplusroadmap 21:04 < kanzure> nmz787: sent.. 21:04 < nmz787> OMG did you just see what Bryan Bishop posted to the DIYbio group? 21:04 < nmz787> insanity 21:04 < nmz787> :P 21:05 < kanzure> cathal sent that? 21:05 < kanzure> actually, where the hell is cathal and why does he never come in here 21:06 < nmz787> huh? 21:06 < nmz787> cathal said nothing! 21:06 < kanzure> cathal doesn't seem to be online 21:06 < kanzure> yes, well, he should be 21:07 < gnusha> diyhpluswiki.git: dcda6fd 21:07 < kanzure> where is JayDugger 21:36 < jrayhawk> pinyshell isn't all that complicated; you should probably man rbash to learn what's going on 21:36 < nmz787> why use that over bash 21:36 < jrayhawk> that is bash 21:36 < nmz787> pinyshell? 21:36 < nmz787> ==bash? 21:37 < jrayhawk> yes. it is bash with set +r. you should probably man rbash to learn what's going on. 21:39 < kanzure> nmz787: pinyshell is the default shell on diyhpl.us for users that register from the wiki 21:40 < kanzure> you have a more-able account on the server 21:51 < skorket> biopunk.org hasn't been active in nearly 8 months... 21:52 < kanzure> when splicer shows up you should yell at him 21:52 < kanzure> he sometimes logs in 21:52 < kanzure> (he runs biopunk.org) 21:52 < skorket> not necessarily his fault... 21:53 < skorket> hey kanzure and nms787, how are you thinking of getting micron precision movement for you laser cutter? 21:53 < skorket> whoops, I mean nmz787 21:53 < kanzure> micro stage 21:53 < kanzure> http://diyhpl.us/laser_cutter/laser_cutter 21:53 < kanzure> wait 21:53 < kanzure> http://diyhpl.us/laser_etcher/laser_etcher 21:55 < skorket> maybe a bit too expensive, but sparkfun has some steppers that are 0.9 Deg 21:57 < skorket> how close are you guys to building? 21:57 < kanzure> well, i have to order parts 21:57 < kanzure> and fenn has to figure out if he wants to build it 21:58 < kanzure> and nmz787 has to figure out where he is living 21:59 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has quit [Quit: brb] 22:00 < skorket> in case you're interested: https://www.sparkfun.com/products/10846 22:01 < skorket> compatible with the a4988 pololu drivers you're using 22:09 < nmz787> i don't think fenn wants to build it. does the austin hackerspace have a machine shop? 22:09 < nmz787> skorket: those motors look pretty good 22:09 < nmz787> would give us 2X resolution 22:10 -!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap 22:12 < nmz787> skorket: how do i figure out how many oz/in I need 22:14 < kanzure> atx hackerspace is not mnuch of a machine shop 22:15 < kanzure> btw there's #austinhackerspace 22:17 < nmz787> do you have access to that one? 22:18 < nmz787> oh you're not saying there are two in austin 22:18 < nmz787> well fenn said he might head to austin if he has a car there 22:18 < nmz787> soooo 22:18 < nmz787> 3-man hackathon 22:18 < nmz787> for this thing 22:24 < klafka> i was just at the oakland hackerspace 22:24 < klafka> i keep going there for like 10 mins and leaving 22:34 < brownies> oakland has a hackerspace? 22:34 < brownies> where? 22:41 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 22:41 < klafka> sudoroom 22:41 < klafka> on broadway 22:41 < klafka> http://sudoroom.org/ 22:48 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Quit: rmcl] 22:49 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has joined ##hplusroadmap 23:03 -!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Quit: rmcl] 23:13 < brownies> word, thanks. might drop by sometime. 23:13 < klafka> they have meetings on mondays 23:13 < klafka> are you in oakland? 23:14 -!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 272 seconds] 23:24 -!- devrando1 [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 23:33 -!- devrando1 [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Quit: leaving] 23:34 -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap 23:36 < skorket> nmz787, do you mean (oz * in)? 23:41 < brownies> klafka: i'm around, sometimes. 23:59 < brownies> this is literally the most useless web site in the world --- Log closed Sat Aug 04 00:00:28 2012