--- Log opened Fri Apr 12 00:00:55 2013 00:08 <@kanzure> someone stole my fucking github contributions calendar idea https://github.com/will 00:08 <@kanzure> release the pitchforks 00:10 <@kanzure> oh well at least he did it better 00:24 < brownies> what does it say? 00:24 < brownies> oh. "Will" ? 00:29 -!- kmo [100@d162-86.icpnet.pl] has joined ##hplusroadmap 00:29 -!- kmo [100@d162-86.icpnet.pl] has quit [Changing host] 00:29 -!- kmo [100@unaffiliated/kmo] has joined ##hplusroadmap 00:32 <@kanzure> brownies: seems to. 00:32 <@kanzure> or possibly illwi 00:39 < brownies> ah yes. that does make more sense. 00:59 -!- kmo [100@unaffiliated/kmo] has quit [Ping timeout: 264 seconds] 01:09 -!- kmo [100@83-238-74-161.ip.netia.com.pl] has joined ##hplusroadmap 01:09 -!- kmo [100@83-238-74-161.ip.netia.com.pl] has quit [Changing host] 01:09 -!- kmo [100@unaffiliated/kmo] has joined ##hplusroadmap 01:50 -!- makoLime [~mako@103-9-42-32.flip.co.nz] has quit [Ping timeout: 255 seconds] 01:54 -!- HEx1 [~HEx@hexwab.plus.com] has quit [Ping timeout: 256 seconds] 02:02 -!- kmo [100@unaffiliated/kmo] has quit [Ping timeout: 256 seconds] 02:11 -!- cpopell [~cpopell@pool-96-231-37-73.washdc.fios.verizon.net] has quit [Ping timeout: 245 seconds] 02:17 -!- kmo [122@unaffiliated/kmo] has 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kajetan [~kmo@87-205-25-23.ip.netia.com.pl] has joined ##hplusroadmap 10:26 -!- panax [panax@131.247.116.56] has quit [Read error: Connection reset by peer] 10:29 -!- panax [~panax@68.200.160.182] has joined ##hplusroadmap 10:36 -!- eudoxia_ [~eudoxia@r190-134-3-169.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 10:36 -!- eudoxia_ is now known as eudoxia 10:53 <@kanzure> kajetan: hello. 11:03 < eudoxia> so kanz now that you've been outdone, what was your github contributions calendar thingee supposed to say? 11:03 <@kanzure> "HIRE ME" but then i realized that was the last thing i wanted 11:03 < eudoxia> hehe 11:03 < brownies> "HIRE ME BUT ONLY IF THE JOB MEETS THE FOLLOWING EIGHT QUALIFICATIONS:" 11:03 <@kanzure> also his idea of using gradients is better than my idea of just doing darker cells 11:04 < eudoxia> and inject a little javascript that hacks the canvas and makes the text scroll 11:19 -!- eudoxia [~eudoxia@r190-134-3-169.dialup.adsl.anteldata.net.uy] has quit [Remote host closed the connection] 11:29 <@archels> https://gist.github.com/anonymous/5374094 11:35 -!- makoLime [~mako@103-9-42-32.flip.co.nz] has joined ##hplusroadmap 11:38 -!- cpopell [~cpopell@pool-96-231-37-73.washdc.fios.verizon.net] has joined ##hplusroadmap 11:40 -!- Helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Remote host closed the connection] 12:05 <@kanzure> /win 13 12:05 <@kanzure> aacckkk 12:07 <@ParahSailin> needs to be a way to use alt- something to get past window 10 12:11 <@kanzure> there is. you can use alt-letters. 12:11 <@kanzure> except they are sometimes mapped to gnome things. 12:12 <@kanzure> also alt-n is mapped to actual windows in my terminal. 12:53 < kajetan> kanzure, hi, hello 12:57 < jrayhawk> archels: the Surface Pro looks okay 13:00 < jrayhawk> Chromebook Pixel might also do 13:00 <@archels> jrayhawk: thanks, but it violates the storage space requirement 13:00 <@archels> yeah the Pixel would be really nice, but it's not available yet in NL 13:00 < jrayhawk> oh, right, missed that part. sorry. 13:01 < jrayhawk> high density displays are hard to find on the x86 market :( 13:02 < jrayhawk> i guess there's also the macbook pro 13" retina displays 13:02 <@archels> yep, but no touchscreen 13:03 <@fenn> why "no USB 2.0 ports" 13:03 <@archels> I think that 1920x1080 will probably be sufficient for 13.3" and below though 13:03 <@archels> because USB 3.0 13:03 <@archels> I don't want to invest in already-outdated hardware 13:04 < jrayhawk> Is x86 actually a requirement? There's crap like the Transformer Pad Infinity 13:06 < jrayhawk> oh, i guess that's bettery under the screen 13:06 < jrayhawk> nevermind 13:08 <@archels> sadly Windows 8 does not run natively on ARM yet 13:09 <@archels> s/yet// 13:10 < jrayhawk> SVZ131190X? 13:10 < jrayhawk> would only work if the drives are replaceable 13:16 <@archels> looks like a yes. Quite a feat of engineering, there's also an optical drive in there. http://forum.notebookreview.com/sony/274083-photos-taking-vaio-z-apart.html 13:17 < jrayhawk> huh, cool 13:17 < nmz787> paperbot: http://www.computer.org/csdl/trans/tp/preprint/06487512-abs.html 13:17 < paperbot> error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/16fa585bb1ec67fec704da356a620bee.txt 13:18 < jrayhawk> that is a comically dinky mainboard 13:20 <@archels> jrayhawk: the Asus Zenbook Prime is currently on top of the list 13:23 -!- klafka [~klafka@ip-64-134-66-79.public.wayport.net] has joined ##hplusroadmap 13:28 -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap 13:33 <@archels> but if you come across a good alternative do let me know, I appreciate the input 13:40 < jrayhawk> Yeah, most of the nerds I know are looking for pixel-dense x86s, but it's a remarkably underserved market 13:41 < jrayhawk> unfortunately most of them are trackpoint-addicted 13:41 < jrayhawk> only boring business laptops have those anymore 13:41 -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Quit: Leaving] 13:41 -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap 13:43 -!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Remote host closed the connection] 13:52 < jrayhawk> https://github.com/panicsteve/cloud-to-butt 13:57 -!- Viper168_ [~Viper@unaffiliated/viper168] has quit [Ping timeout: 256 seconds] 14:14 -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap 14:15 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 14:16 -!- augur [~augur@208.58.5.87] has quit [Read error: Connection reset by peer] 14:16 -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap 14:19 -!- klafka [~klafka@ip-64-134-66-79.public.wayport.net] has quit [Remote host closed the connection] 14:33 -!- Juul [~Juul@50-0-83-116.dsl.static.sonic.net] has joined ##hplusroadmap 14:36 -!- panax [~panax@68.200.160.182] has quit [] 15:07 -!- ielo [~ielo@cpc9-addl4-2-0-cust229.6-3.cable.virginmedia.com] has joined ##hplusroadmap 15:25 -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] 15:32 < juri_> HR displays? look toward fujitsu. 15:43 < jrayhawk> i haven't seen anything particularly impressive from the lifebook line; what did you have in mind 16:10 -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -] 16:15 < juri_> i've seen some impressive stuff from them in the past.. but you have to order it from their japanese site. 16:19 -!- FooQuuxman [~arik@c-98-215-254-55.hsd1.il.comcast.net] has quit [Ping timeout: 240 seconds] 16:24 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has joined ##hplusroadmap 16:26 -!- ielo [~ielo@cpc9-addl4-2-0-cust229.6-3.cable.virginmedia.com] has quit [Ping timeout: 256 seconds] 16:42 -!- yorick [~yorick@oftn/member/yorick] has quit [Read error: Connection reset by peer] 16:43 < nmz787> kanzure: if you want something to watch these talks are really fun http://alumni.media.mit.edu/~dlanman/ 17:22 < nsh> is bout what? 17:22 <@kanzure> i wonder if httpetrified/httprettified/httpretty works with libmproxy 17:23 < nsh> reminds me of the guy hwo did the "magnets how do they work?!!" talk 17:23 <@kanzure> attached wsgi apps bypass handle_request.. which was not what i intended. 17:23 < nsh> which the crazy modelling system 17:24 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] 17:24 -!- FooQuuxman [~arik@c-98-215-254-53.hsd1.il.comcast.net] has joined ##hplusroadmap 17:29 <@kanzure> nmz787: well here's one answer... 17:29 <@kanzure> 17:26 <@ggreer> kanzure: going for a run. we can pair after that 17:29 <@kanzure> 17:27 <@ggreer> err 17:29 <@kanzure> 17:27 <@ggreer> sorry wrong channel. and wrong person. autocomplete. 17:29 <@kanzure> 17:27 <@ggreer> (kansface is the nick of my cofounder. we usually pair program in the evenings) 17:31 -!- eudoxia [~eudoxia@r186-52-168-84.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 17:41 -!- AlonzoTG [~atg@pool-108-28-74-221.washdc.fios.verizon.net] has quit [Read error: Connection reset by peer] 17:46 < ParahSail1n> lol kansface 17:47 <@kanzure> ParahSail1n: because of 17:47 <@kanzure> 2013-04-09.log:14:41 < nmz787> i wonder if kansface is some attempt to digitally slap you kanzure 17:47 <@kanzure> from https://news.ycombinator.com/item?id=5519676 17:47 -!- AlonzoTG [~atg@pool-108-28-74-221.washdc.fios.verizon.net] has joined ##hplusroadmap 17:47 < eudoxia> it's the face kanzure makes when sending hatemail 17:47 < eudoxia> le angry kanzure face 17:48 <@kanzure> all email is hatemail 17:48 -!- schizophrenic [~HymnToThe@2601:7:5700:68:1596:40b6:d24f:7cc8] has joined ##hplusroadmap 17:50 -!- Juul [~Juul@50-0-83-116.dsl.static.sonic.net] has quit [Ping timeout: 248 seconds] 17:51 -!- eudoxia [~eudoxia@r186-52-168-84.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] 17:56 -!- Juul [~Juul@50-0-83-116.dsl.static.sonic.net] has joined ##hplusroadmap 18:20 -!- FooQuuxm1n [~arik@c-98-215-254-55.hsd1.il.comcast.net] has joined ##hplusroadmap 18:21 -!- FooQuuxman [~arik@c-98-215-254-53.hsd1.il.comcast.net] has quit [Ping timeout: 260 seconds] 18:21 -!- FooQuuxm1n is now known as FooQuuxman 18:25 -!- Replop [~Gwen@ASte-Genev-Bois-154-1-19-170.w81-249.abo.wanadoo.fr] has quit [Quit: Leaving.] 18:36 -!- schizophrenic [~HymnToThe@2601:7:5700:68:1596:40b6:d24f:7cc8] has quit [Quit: Leaving] 18:46 -!- Juul [~Juul@50-0-83-116.dsl.static.sonic.net] has quit [Read error: Operation timed out] 18:49 <@fenn> Injectable, Cellular-Scale Optoelectronics with Applications for Wireless Optogenetics http://www.sciencemag.org/content/340/6129/211.abstract 18:50 <@fenn> paperbot: http://www.sciencemag.org/content/340/6129/211.full 18:50 < paperbot> error: HTTP 300 http://diyhpl.us/~bryan/papers2/paperbot/Injectable%2C%20Cellular-Scale%20Optoelectronics%20with%20Applications%20for%20Wireless%20Optogenetics.pdf 18:53 < ParahSail1n> has anyone built a lyman extruder? 18:59 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Ping timeout: 255 seconds] 19:00 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap 19:05 -!- lichen_ [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap 19:05 -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Ping timeout: 255 seconds] 19:09 -!- makoLime [~mako@103-9-42-32.flip.co.nz] has quit [Quit: Leaving] 19:26 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 19:31 < ParahSail1n> what, wood filament? http://www.lulzbot.com/?q=products/laywoo-d3-wood-filament-3mm 19:49 -!- wrldpc [~wrldpc@120.88.48.129] has quit [Quit: wrldpc] 20:35 -!- klafka [~klafka@cpe-98-14-144-138.nyc.res.rr.com] has joined ##hplusroadmap 20:46 -!- klafka [~klafka@cpe-98-14-144-138.nyc.res.rr.com] has quit [Remote host closed the connection] 20:46 -!- cpopell [~cpopell@pool-96-231-37-73.washdc.fios.verizon.net] has quit [Ping timeout: 256 seconds] 21:04 -!- lichen_ [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Quit: Lost terminal] 21:13 < jrayhawk> huh 21:17 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: the neuronal action potential is an electrical manipulation of reversible abrupt phase changes in the lipid bilayer] 21:35 -!- FooQuuxman [~arik@c-98-215-254-55.hsd1.il.comcast.net] has quit [Ping timeout: 245 seconds] 21:56 -!- klafka [~klafka@cpe-98-14-144-138.nyc.res.rr.com] has joined ##hplusroadmap 22:01 -!- klafka [~klafka@cpe-98-14-144-138.nyc.res.rr.com] has quit [Ping timeout: 245 seconds] 22:05 -!- aristarchus [~aristarch@unaffiliated/aristarchus] has quit [Quit: Leaving] 22:12 -!- cpopell [~cpopell@pool-96-231-37-73.washdc.fios.verizon.net] has joined ##hplusroadmap 22:18 -!- cpopell [~cpopell@pool-96-231-37-73.washdc.fios.verizon.net] has quit [Ping timeout: 243 seconds] 22:29 -!- plur [~nonentity@121-73-87-49.cable.telstraclear.net] has quit [Ping timeout: 245 seconds] 22:29 -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has joined ##hplusroadmap 22:30 <@kanzure> yashgaroth: lab update? 22:30 < yashgaroth> last meeting was a weird sbir grant-writing-brainstorming-clusterfuck 22:30 <@kanzure> ha ha sbir 22:30 < yashgaroth> the one guy who brought in a couple 3d printers kept sassing the entire meeting 22:30 <@kanzure> a heckler? 22:31 < yashgaroth> sorta, yeah, like 'oh I've applied for plenty of sbir's and it's a crapshoot and these ideas are stupid' 22:31 <@kanzure> nice 22:31 <@kanzure> he's my hero 22:31 < yashgaroth> admittedly he wasn't wrong 22:31 <@kanzure> shouldn't kevin lustig have some pharma connections that could drop a few hundred grand on it? 22:31 < yashgaroth> but then he was at odds with the guy who brought in the 28-foot van full of lab equipment, who was all offering to help review grant applications and such 22:32 < yashgaroth> kevin wasn't even there for this one, it was odd 22:32 <@kanzure> i feel like kevin should have some dark sith connections or some shit 22:32 < yashgaroth> idk if kevin has pharma connections outside of his assaydepot-related stuff 22:32 <@kanzure> well even if it's through assaydepot 22:32 <@kanzure> i mean, that's probably how he knows pharma people 22:32 < yashgaroth> aside from the whole 'PhD from UCSF' which I assume is good for at least a dozen primo connectz 22:32 <@kanzure> haha.. no. 22:33 < yashgaroth> yeeeah 22:33 < yashgaroth> also kevin not being there meant no food and barely any alcohol, which I think hurt it more than any heckling 22:33 <@kanzure> i also feel like ryan bethencourt probably knows a bunch of san diego people with deep pockets 22:33 -!- jrayhawk [~jrayhawk@nursie.omgwallhack.org] has quit [Read error: Connection reset by peer] 22:33 < yashgaroth> who is 22:33 <@kanzure> but ryan is probably trying to get them to fund east bay diybio or his fibroalzheimerohshit npo 22:33 < yashgaroth> nm google's filling me in 22:34 <@kanzure> ryan is just some pharma/biotech sales guy who hangs out in the community 22:34 < yashgaroth> there was talk about a peter blank I think 22:34 <@kanzure> i'm not completely sure about his background, there's supposed to be other things, he's been around in diybio/transhumanism since forever 22:34 < yashgaroth> something * blank, some bigwig business silicon valley guy 22:34 < yashgaroth> yeah I'd heard the name 22:34 <@kanzure> "- Humanity + Caltech conference (2010) - talk titled "2020 the Future of the Biopharmaceutical industry "" 22:34 <@kanzure> ""Open, cheap and fast: New Models of biotechnology entrepreneurship" " 22:34 <@kanzure> "- Singularity University, Biotech track, July 2012" 22:34 <@kanzure> "- Open Science Summit 2012, “Guerilla” style biotech for ALS" 22:35 <@kanzure> "Co-founder, Rawflow.com - VC funded by Benchmark Capital £2M" 22:35 <@kanzure> "Genescient Pharma division - Adviser and former P/T COO (helped close first external angel round)" 22:35 <@kanzure> wtf genescient too? well ok. 22:35 <@kanzure> ah right pfizer sales 22:35 <@kanzure> well anyway. he probably knows people who hang out in san diego and spend lots of biotech money. 22:35 < yashgaroth> "combines evolutionary genomics with massive selective screening to analyze and exploit the" hmm yeah 22:35 < yashgaroth> I defer all bigwig-knowingness to jojack 22:36 < yashgaroth> and I thought the whole meeting would be about writing an sbir for the actual lab, but everyone all like 'oh who wants to write that pfft' 22:36 <@kanzure> jojack has a really weird ability to get around and find the right people, but the deals don't always close 22:36 <@kanzure> yeah, i think for a sbir you might want to consider just hiring someone else to write it 22:37 < yashgaroth> with all that money we have 22:37 <@kanzure> i think grants are leagues better than membership fees for the hackerspace 22:37 <@kanzure> but grants also suck in the scheme of things 22:38 < yashgaroth> yeah I dunno what the actual output requirements are for grants like that 22:38 <@kanzure> in terms of bang-for-the-buck i think the gates foundation grants are worth pursuing in your situation 22:38 < ParahSail1n> do you know anyone who's gotten gates bucks? 22:39 <@kanzure> darren zhu 22:39 < yashgaroth> jojack seems unwilling to write the grants for some reason 22:39 < yashgaroth> maybe he's bad at writing, who knows 22:39 <@kanzure> actually my records indicate that i know darren via jojack, weird 22:39 <@kanzure> no, he's very good at writing (but i think this is because he spends an extreme amount of time at it because obsession) 22:40 <@kanzure> evidence: http://jetpress.org/v19/jackson.pdf 22:41 < yashgaroth> well there you go, but when I mention writing the application he's very dismissive 22:42 < yashgaroth> sure it's not a traditional sbir type of proposal, but hey 22:42 < yashgaroth> also idk if I mentioned but jcline thinks there's big money in kombucha 22:43 <@kanzure> something about algae? 22:43 < yashgaroth> and algae, but kombucha is like yeast + bacteria forming these globs that ferment tea or some shit 22:43 < yashgaroth> it's the new wholefoods thing 22:44 <@kanzure> genspace likes to make money by giving presentations in-house 22:45 <@kanzure> but this ends up hijacking a big portion of the space (lots of chairs, lots of scheduling issues, etc.) 22:45 <@kanzure> in san diego i think it would be more efficient to bring the presentations to different companies that want to support a hackerspace 22:45 <@kanzure> in exchange they would get professional talks about cool biotechnology projects 22:45 <@kanzure> uh i mean in exchange for their bags of money per presentation 22:45 <@kanzure> or training session (off site) 22:45 < yashgaroth> very few of these companies care about hackerspaces, and who would be talking at these talks? 22:46 < yashgaroth> because if it's our usual bunch of presentations like last night, it'll just be 'so we get some stem cells right, and then alzheimers is cured' 22:46 < yashgaroth> because stem cells, you see 22:46 <@kanzure> well, not jojack because he doesn't seem to think he's capable of giving highly technical talks 22:47 < yashgaroth> about biotechnology? then I might agree with him to be honest 22:47 <@kanzure> heh 22:47 <@kanzure> he really needs to sit down and get to the point where he feels confident about biotechnology 22:47 <@kanzure> he just... hasn't? for some reason? 22:48 <@kanzure> anyway, i dunno what the mechanics would be. but speaking at companies is a known thing that happens that they might be receptive to. you could possibly recruit other people with careers and give them free publicity in the process. dunno. 22:49 < yashgaroth> well jojack could organize that pretty easily, but most companies have a tech/patent that they're pursuing, and ignore anything else unless it's a direct and immediate competitor to them 22:49 < yashgaroth> at least in biotech I guess 22:49 <@kanzure> uh by recruit i mean "hey go speak at this event on behalf of jerkwad biohack incorporated, in exchange you get your name out to these companies and we get some money to support the space". and then they go off and give some talk about whatever biotech topic. 22:50 <@kanzure> yeah, i don't know how bad it is in the biotech industry 22:50 <@kanzure> i know there's a lot of companies that just hire pipette drones 22:50 <@kanzure> which would be less receptive. 22:50 < yashgaroth> well they need a few paper drones at the top 22:51 <@kanzure> what sorta biotech company density is there in san diego anyway? 22:51 < yashgaroth> generally the talk culture is conference-oriented, where if you've been a good little drone you get to fly to cincinnati for proteinchat 2013 22:51 < yashgaroth> density? super high 22:51 < yashgaroth> mira mesa/sorrento valley, every other building is biotech 22:53 < yashgaroth> ah apparently sorrento mesa is the region name 22:57 <@kanzure> i guess gates foundation is my only immediately-relevant suggestion. i think they expect just one or two pages of typed information on their application. 22:58 < yashgaroth> on paper the whole thing looks like a great funding recipient 22:59 < brownies> what're you trying to fund? 22:59 <@kanzure> does it? i expect that (for gates foundation money) there would be some specific project that would require evidence of at least one or two people being committed to the project, its progress and completion. i'm not sure if you can demonstrate that at the moment? 22:59 < yashgaroth> our local diybio lab space 22:59 < yashgaroth> (brownies) 23:00 <@kanzure> the city of san diego granted world heavyweight champion jojack a $1 lease on a 6000 sq ft lab facility for biohacking adventures 23:00 < yashgaroth> we have a whole bunch of half-baked projects that could occur in the space, I guess, so those mashed together may add up to one good project 23:00 < yashgaroth> the city of carlsbad, ahem 23:00 < brownies> heh really? that's pretty awesome 23:00 <@kanzure> and now they are trying to figure out how to make money 23:01 < yashgaroth> also it's not a lab facility yet, just an office building 23:01 < brownies> lease me half of it for $3 23:01 < brownies> that's a 400% ROI 23:01 < yashgaroth> add three zeros to that and you've got a deal 23:01 <@kanzure> $0.003 23:01 <@kanzure> done 23:01 < yashgaroth> sold! 23:01 < yashgaroth> your square inch of leased space is bound by all applicable restrictions 23:02 < yashgaroth> ...once we figure out what those are 23:02 <@kanzure> "code of ethics" 23:02 < yashgaroth> pffft haha 23:02 < brownies> other than stuffing it full of lab equipment of some sort, what kinds of things are planned? 23:03 < brownies> you could stuff it full of lab equipment and then turn it into a biocoworking space? rent out things to aspiring biotech startups? 23:03 < yashgaroth> we've already stuffed it full of equipment, we need to tear up the existant carpet which is a no-no for labwork 23:03 < brownies> perhaps you should've done that before the stuffing 23:03 < yashgaroth> that was the original idea, hosting a bunch of startups 23:03 <@kanzure> i think the problem with renting it out to biotech companies is that it quickly becomes a typical lab renting facility.. which is sorta boring and not going to end up facilitating yashgaroth's projects. 23:03 < yashgaroth> ehh we can always stuff the stuff 23:04 < yashgaroth> at this point I'd be okay with a studio apartment 23:04 <@kanzure> and then they stop sharing equipment because it has to be calibrated perfectly for each company 23:04 <@kanzure> and because SECRETS 23:04 < yashgaroth> jcline's gonna help me with a bottle bioreactor, don't really *need* a centrifuge if I get creative 23:04 <@kanzure> biotech startups are remarkably different in terms of their attitudes and beliefs 23:04 < brownies> what kinds of things are " yashgaroth 's projects" 23:04 < yashgaroth> yeah it's mostly secrets, and heavy mistrust of all other companies 23:04 <@kanzure> direct electroporation of human muscle tissue 23:05 <@kanzure> whole body electrophoresis 23:05 < yashgaroth> "diy human genetic engineering" 23:05 < yashgaroth> with plasmids that produce whatever gene/protein you would like more of 23:05 < brownies> i think it's time for you to really buckle down and focus on serious work 23:05 < brownies> it's been weeks and i still don't have a glowing cat 23:06 < yashgaroth> you're right, I'll stem some cells 23:06 < yashgaroth> honestly all I'd need from the official space is a place to ship reagents to, since many places want a commercial address 23:07 < yashgaroth> but somewhere deep in my heart the dream of an actual diybio lab still lives 23:07 < yashgaroth> despite how shit-useless the community is 23:07 <@kanzure> my recommendation is to focus on specific projects 23:07 <@kanzure> which seems to be the way that the los angeles group works 23:07 <@kanzure> cory seems to get a lot of fun things done using this approach 23:08 < brownies> what kinds of projects do they do? 23:08 <@kanzure> although he often doesn't share what he's been up to until later 23:08 < yashgaroth> I like his project even if it'll never go anywhere 23:08 <@kanzure> well he has a neural tissue culture somewhere 23:08 <@kanzure> and a deal with the chinese government to sequence basically anything he gives them 23:08 < yashgaroth> his big thing is a biological haber process 23:08 <@kanzure> and his nitrogenase high-pressure tank for directed selection? iirc. 23:09 <@kanzure> nitrogenase? i hope i am remembering the right enzyme he's playing with.. 23:09 < yashgaroth> well that's all a part of it, and yes that's the one 23:09 < ParahSail1n> who's this? 23:09 < yashgaroth> cory tobin 23:09 <@kanzure> cory tobin at los angeles biohackers 23:09 <@kanzure> http://www.biohackers.la/ 23:09 <@kanzure> http://wiki.biohackers.la/Main_Page 23:09 < yashgaroth> he apparently found a nitrogen-fixing bacterium in german charcoal pits that can fix N2 in the presence of oxygen 23:10 < ParahSail1n> that's thermodynamically impossible 23:10 < yashgaroth> or rather, read some abandoned research about it 23:10 < ParahSail1n> like as in delta G shit 23:10 < yashgaroth> yeah well don't let thermodynamics get in the way of things 23:11 < yashgaroth> it's this bizarre species that only lives at like 60C or some shit 23:11 <@kanzure> yeah he described his setup once and it seemed.. less than safe. 23:11 <@kanzure> "Project Hindenburg -> streptomyces thermoautotrophicus" 23:11 < yashgaroth> whole lotta H2 being pumped in 23:11 <@kanzure> "hydrogen + oxygen growth chamber at 50 psi, explosive and very volatile, so we don't keep it in doors" 23:12 <@kanzure> "Project Skynet -> Reverse engineering an in vivo neural network on a chip" 23:12 -!- cory_ [475c4761@gateway/web/freenode/ip.71.92.71.97] has joined ##hplusroadmap 23:12 < rigel> how on earth could that be useful anyway 23:12 <@kanzure> cory_: hi, we were discussing streptomyces thermoautotrophicus 23:13 < rigel> if it requires h2 23:13 <@kanzure> ParahSail1n: please elaborate on your thermodynamic violation claims 23:13 < cory_> it can consume either CO or a combination of H2 and CO2 23:13 < ParahSail1n> kanzure, brb im looking up the crc tables 23:14 <@kanzure> too bad crc is so god damn inaccessible 23:15 <@kanzure> cory_: we were also trying to figure out what projects we should make the carslbad people look into 23:15 <@kanzure> *carlsbad 23:16 < yashgaroth> yea we're down to collaborate 23:17 < cory_> what kind of projects are you/they looking for? Like biotech startup type projects? 23:17 < yashgaroth> sure, we haven't really decided on a theme yet 23:18 < yashgaroth> biotech incubator, membership+rented bench space, teaching classes, any or all of those 23:18 <@kanzure> do you need to focus on money? 23:18 < yashgaroth> according to jojack, oh god yes 23:18 <@kanzure> because carlsbad will kick you out? 23:18 < yashgaroth> eventually, but the electric's only free for a year and -80s are power hogs 23:19 < yashgaroth> I don't know how often the city's gonna come by and check on how we're progressing, what with us promising 8+ startups 23:19 <@kanzure> haha wait what else have you promised 23:19 < yashgaroth> damn I used to have the powerpoint somewhere 23:19 < yashgaroth> ask jojack, he did the presentation 23:20 < yashgaroth> general fostering innovation and whatnot, but the 8 startups within X years was the big one 23:21 <@kanzure> out of curiosity... what? like 8 startups owned by the space? 23:21 < brownies> perhaps you should talk to the local universities? 23:21 < brownies> they're always producing startups 'n' things right? 23:21 < yashgaroth> 8 inhabiting the space, paying rent and producing patentz 23:22 <@kanzure> oh.. so it looks like your plan is already set then. good luck :P 23:22 < yashgaroth> we're in talks with the local uni's, at least community colleges, but mostly about hosting lab classes for them since they have no space to run their own lab classes 23:22 < yashgaroth> idk how many startups a CC will produce per year 23:23 < yashgaroth> I guess there's enough biologists here that, say, someone with a marginal idea that wouldn't get VC funding would be able to maybe get off the ground at our space 23:23 < ParahSail1n> hm, delta G comes out negative 23:23 < ParahSail1n> i guess i was wrong 23:24 < ParahSail1n> its just a question of activation energy rather than favorability 23:25 < cory_> @parahsailin is this in regard to the nitrogenase? 23:25 < ParahSail1n> yes 23:25 < cory_> what were you calculating the delta-G of? 23:26 < ParahSail1n> 1/2 N2 + 3/2 H2 -> NH3 23:27 < cory_> oh, well that's not the reaction, it doesn't use H2 directly to do the reaction, I was just saying the bacteria either metabolizes CO or H2+CO2 23:27 < cory_> I'll go look up the full reaction, brb 23:28 < ParahSail1n> whatever the german guy's name for the law that chemical reactions are path independent 23:29 < ParahSail1n> thats a cool sounding bug you got your hands on 23:31 < ParahSail1n> oh that's interesting, CO is used as the electron donor and H+ and N2 are electron acceptors 23:32 < cory_> http://i.imgur.com/Ncg4qZW.png and http://i.imgur.com/dHAMdQj.png 23:33 < cory_> yeah, it's a pretty cool bug. Pain in the ass to grow though. Blew up one bioreactor before I got it right. 23:34 < cory_> It doesn't grow on any sort of carbon-based media. Won't eat sugars, carbohydrates, fats, etc. Only toxic gas :P 23:35 < cory_> *hot toxic explosive gas 23:36 < ParahSail1n> http://imgur.com/beJ7pAQ 23:37 < ParahSail1n> seems like a bit of a curiosity unless you have carbon monoxide source 23:38 < ParahSail1n> CO is its primary substrate? 23:38 < cory_> I think the CO-dehydrogenase is just there to produce more superoxide. I don't think the CO is critical for nitrogenase functionality since cells generate superoxide 23:38 < ParahSail1n> for growth i mean? 23:39 < cory_> yeah, but it can also consume a combination of H2 and CO2 23:39 <@kanzure> paperbot: http://www.jbc.org/content/272/42/26627.short 23:39 < paperbot> http://diyhpl.us/~bryan/papers2/paperbot/N2%20Fixation%20by%20Streptomyces%20thermoautotrophicus%20Involves%20a%20Molybdenum-Dinitrogenase%20and%20a%20Manganese-Superoxide%20Oxidoreductase%20That%20Couple%20N2Reduction%20to%20the%20Oxidation%20of%20Superoxide%20Produced%20from%20O2by%20a%20Molybdenum-CO%20Dehydrogenase.pdf 23:40 < cory_> in the case of CO it uses CO as both an energy souce and carbon source. Or it uses H2 for energy and CO2 for carbon since it has RuBisCO 23:41 < yashgaroth> wait wtf it's photosynthetic too? 23:43 < yashgaroth> oh wait nevermind 23:43 < cory_> no, but it can convert CO2 to sugar. Photosynthesis and CO2 sequestration aren't directly coupled 23:44 < yashgaroth> right right I forgot that bit 23:48 < ParahSail1n> its gotta keep O2 away from the nitrogenase otherwise it's just going to reduce it? 23:49 < ParahSail1n> i think it has to sequester that nitrogenase in a low oxygen region like cyanobacteria do 23:50 < cory_> well, in the classical nitrogenases the enzymes were poisoned by O2, specifically the O2 oxidized the Fe cofactor or something like that. It is possible that this bug's nitrogenase is immune to O2 poisoning. But I haven't got that far yet. Still working on setting up biochemical experiments. 23:50 < ParahSail1n> if the catalytic center is capable of activating nitrogen, it's gonna activate oxygen too 23:51 < cory_> yeah maybe. but it may be able to do so without destroying the enzyme. 23:51 < cory_> which is the case in most nitrogenases 23:52 < cory_> The original work on this bug showed that the nitrogenase works in vitro in the presence of oxygen 23:53 < cory_> but there is a lot of skepticisim that the work was nto wrong or fraudulent. I'm trying to re-do those experiments now 23:53 < ParahSail1n> you expressing nitrogenase? 23:54 < cory_> no, just purifying it from S. thermoautotrophicus using sepharose columns 23:55 < ParahSail1n> get that bug sequenced 23:55 <@kanzure> haha well.. 23:55 < cory_> already did :) 23:55 < cory_> still assembling it. It's like 78% GC 23:55 < ParahSail1n> you got a server with the reads? 23:55 <@kanzure> could we get a copy of the reads? 23:55 <@kanzure> yeah 23:56 < ParahSail1n> ill assemble that shit 23:57 < cory_> they're on some server at harvard. I'll ask my collaborators for the link. We're re-doing the sequencing now because the original sample seems to be a micture of multiple strains 23:57 < ParahSail1n> illumina? 23:57 < cory_> We're working on getting a purer line going to sequence. 23:57 < cory_> yeah 23:57 < ParahSail1n> i have some tools that work well on microbiomes 23:57 < cory_> but we're also going to do some 454 or pacbio to get some longer reads 23:57 < ParahSail1n> velvet chokes on those 23:58 < ParahSail1n> nah you dont need that, just get lots of coverage on hiseq 23:58 <@kanzure> what is lots of coverage? 30x? 23:58 < ParahSail1n> 30x is a little low for bacteria 23:59 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 23:59 < cory_> well, in the first run we had some areas with 100x coverage and some with zero. so we were hoping to maybe stitch together some of those gaps with longer reads 23:59 < ParahSail1n> usually aim for a couple hundred 23:59 <@kanzure> couple hundred :\ 23:59 < ParahSail1n> couple hundred is like 2% of a hiseq lane 23:59 < ParahSail1n> hiseq lane is like $3000 23:59 <@kanzure> haha i don't have a hiseq machine laying around --- Log closed Sat Apr 13 00:00:02 2013