--- Log opened Wed May 01 00:00:13 2013
00:15 < kanzure> ryankarason: also, you can pick them up pretty cheap on ebay and alibaba
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02:25 < archels> http://blaauw.eecs.umich.edu/project.php?id=66&sid=d4c39954d090d21e5974cfcfa0324ded
02:25 < archels> .title
02:25 < yoleaux> archels: Sorry, that doesn't appear to be an HTML page.
02:26 < archels> no botsnacks for you, yoleaux
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03:13 < kanzure> hfempire seems to let you buy DDOS attacks. not sure.. it doesn't ask you for the target.
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07:40 < ryankarason> kanzure: what exactly would i be looking for, a "dna sequencing machine" ? and are there any diy tutorials for building one ?
07:55 < kanzure> yes, you would type in "dna sequencer"
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08:21 < ryankarason> kanzure: what would you say is "pretty cheap"
08:21 < ryankarason> and also, do you have a dna sequencer?
08:28 < kanzure> pretty cheap is anything under $10,000 in this case
08:28 < kanzure> no, i don't presently have a dna sequencer
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10:11 < ParahSai1in> http://bostonherald.com/business/technology/technology_news/2013/04/genetic_research_toolmaker_raindance_technologies_raises
10:13 < kanzure> "and the recently launched RainDrop digital PCR system for ultra-sensitive multiplexed quantitative detection of circulating nucleic acids."
10:13 < kanzure> what does that even mean.. circulating. so it's a thermocycler that you put into your bloodstream?
10:13 < kanzure> or "circulating within the confines of a micropipette tube"
10:14 < kanzure> oh god i don't even remember what to call them. microcentrifuge tubes? micropipette tubes?
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12:06 < archels> Google Glass has competition already - http://tele-pathy.org/
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13:24 < rk[woodsing]> http://openpcr.org/
13:25 < rk[woodsing]> $600, would more be what i would define as "cheap"
13:25 < kanzure> yes we're aware of openpcr. it's really expensive, but cheaper than the other commercial stuff.
13:26 < kanzure> there's also a $75 thermocycler on github called wiremound or something
13:26 < kanzure> and then there's russell's thing, uh, lightbulb pcr something
13:26 < chris_99> why is openpcr so expensive
13:26 < rk[woodsing]> hmm, well why would you say $10k is cheap
13:26 < chris_99> what's the main cost in it
13:27 < chris_99> cheap for a sequencer he said
13:27 < rk[woodsing]> is openpcr a sequencer?
13:27 < chris_99> no
13:27 < rk[woodsing]> what is the difference?
13:27 < chris_99> it just replicates DNA
13:27 < kanzure> wtf chris_99
13:27 < chris_99> if that's the right term
13:27 < kanzure> no it doesn't replicate dna
13:28 < chris_99> sorry what's the right term
13:28 < kanzure> openpcr is a thermocycler. it changes the temperature of small liquid samples.
13:28 < chris_99> *amplifies
13:28 < kanzure> what?
13:36 < rk[woodsing]> so what are the parts/steps for dna sequencing. dna extraction, dna amplification, and dna detection. so some extracting medium, a thermocycler, and what is technology is used for detection ?
13:36 < rk[woodsing]> those are the three steps i understand from my recent readings...
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13:38 < kanzure> i would start by reading up about sanger sequencing, pyrosequencing, next gen sequencing, etc.
13:40 < ryankarason> do you have any good links for such topics, or should i just start the random searching?
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13:44 < kanzure> wikipedia.
13:44 < kanzure> http://en.wikipedia.org/wiki/Sanger_sequencing
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14:09 < kanzure> "in fact, it's illegal to sell water "contaminated" with magnesium"
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17:11 < superkuh> paperbot: http://www.jneurosci.org/content/33/15/6460.full.pdf+html
17:11 < paperbot> no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Increases%20in%20cAMP%2C%20MAPK%20Activity%2C%20and%20CREB%20Phosphorylation%20during%20REM%20Sleep%3A%20Implications%20for%20REM%20Sleep%20and%20Memory%20Consolidation.pdf
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17:42 < kanzure> jmil: hey how goes rice?
17:47 < superkuh> paperbot: http://www.sciencemag.org/content/340/6131/449.long
17:47 < paperbot> error: HTTP 300 http://diyhpl.us/~bryan/papers2/paperbot/Neurotransmitter%20Switching%20in%20the%20Adult%20Brain%20Regulates%20Behavior.pdf
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19:55 < jmil> kanzure: sweeeeeet
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--- Log closed Thu May 02 00:00:02 2013