--- Log opened Sat Jun 22 00:00:04 2013
00:13 < abetusk> So I hope I won't get lashed too harshly for such a general question, but how long, how feasible and what kind of technology needs to happen to get whole exome sequencing at the DIY or home biology level?
00:47 < nmz787_> you can probably do the extraction and prep in current DIY labs
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09:27 < kanzure> Guest81512: you can do that at home now, but you would have to either refurbish a machine or build your own
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09:27 < abetusk> kanzure, what now?
09:29 < yashgaroth> whole exome sequencing
09:30 < abetusk> ah.  So whole exome sequencing is possible at the DIY level?
09:30 < yashgaroth> with the sequencers available on ebay, it's possible but would take a really long time
09:30 < abetusk> um, and maybe I should qualify that with cheap as well...
09:31 < kanzure> refurbishing a sequencer is totes doable
09:31 < kanzure> also, DIY does not necessarily mean cheap. get a job.
09:31 < kanzure> *better job
09:31 < yashgaroth> far cheaper to pay a company than to buy the sequencer/get it working, and all the reagents
09:32 < abetusk> so the answer either way is throw more money at it
09:32 < kanzure> well, you can build a sequencer if you want
09:32 < yashgaroth> as with most of biology, yes
09:34 < kanzure> i wish more people would choose the build option
09:34 < kanzure> we would probably have actually good equipment by now if someone started working on a sequencer 5 years ago or w/e
09:34 < yashgaroth> but but another pcr machine
09:34 < kanzure> hah
09:34 < kanzure> did you ever see jojack's pcr machine?
09:35 < yashgaroth> was he on the lavaamp?
09:35 < kanzure> yes
09:36 < yashgaroth> never really looked into it because it's [another diy pcr machine]
09:36 < kanzure> they spent $100k on it (or more). at least $100k went to rob carlson to make 1 prototype.
09:37 < yashgaroth> hahaha
09:38 < gradstudentbot> The paper was rejected.
09:38 < yashgaroth> "The price point for new LavaAmps should be well underneath the several thousand dollars charged for educational thermocyclers that use heater blocks powered by peltier chips" oh it costs less than several thousand dollars? how did they possibly manage such a feat
09:39 < abetusk> didn't you just show a video where jojack was complaining about inefficiencies?
09:40 < kanzure> well..... yes.
09:40 < yashgaroth> jojack complains about a lot of things, so probably
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10:29 < kanzure> front-end things :( https://gist.github.com/dypsilon/5819504
10:30 < kanzure> i guess i mean https://github.com/dypsilon/frontend-dev-bookmarks but this seems like a dumb way to store bookmarks (in a markdown file in git?)
10:30 < kanzure> and his security stuff is weak. hah.
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10:56 < heath> https://www.youtube.com/watch?feature=player_detailpage&v=AWEpeW7Ojzs#t=308s
10:56 < heath> .title
10:56 < yoleaux> Ellen Jorgensen: Biohacking -- you can do it, too - YouTube
10:56 < heath> where is this United Nations study she mentions here?
11:32 < kanzure> ugh there are makerbot stores in manhattan now
11:32 < kanzure> heath: check the transcripts from fbi-diybio-2012. i think i asked that question and she responded.
12:22 < heath> thanks reading now
12:39 < heath> ok, i don't see anything to do with a report
12:39 < heath> i'll email her
12:47 < brownies> a makerbot *store*? as in, a store dedicated to selling makerbots? doesn't that seem a bit much?
12:48 < kanzure> they probably sell plastic too
12:48 < kanzure> but yes that does seem over-the-top
12:50 < gradstudentbot> That's definitely a Nature paper.
12:51 < kanzure> heath: unfortunately ellen does not believe in email (she only communicates with people she knows in person/sees daily)
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13:23 < kanzure> webgl + articulated models http://alteredqualia.com/three/examples/webgl_animation_skinning_tf2.html
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14:03 < heath> maybe she was talking about fbi-diybio workshop
14:04 < heath> and speaking with piers d millet
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14:06 < heath> https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&ved=0CC8QFjAA&url=http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf&ei=cRHGUdjFHIjw8QSv1IGoDw&usg=AFQjCNFBFRDtE4JqF7K-b5fZK04Wlb7R9A&sig2=Ka_DgC8tHVJ-ioiPSaroEg
14:06 < heath> .titlel
14:06 < heath> .title
14:06 < yoleaux> heath: Sorry, that doesn't appear to be an HTML page.
14:08 < fenn> i thought you installed the extension to get rid of that link tracking crap
14:10 < heath> me?
14:10  * heath isn't aware of an extension
14:10 < heath> i was just using scapy to find the exact URL...
14:12 < fenn> oh nm that was nmz787
14:12 < kanzure> the actual url is in that url. just run it through urldecode
14:12 < kanzure> +.
14:13 < kanzure> or maybe it's uridecode
14:17 < brownies> .meow
14:17 < yoleaux> http://moar.edgecats.net/cats/XgSDwJH.gif
14:17 < heath> http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf
14:17 < kanzure> now pass that through uridecode
14:19 < heath> http://unog.ch/80256EDD006B8954/(httpAssets)/2C8C2466169C45F5C1257770004E66B7/$file/International+perspectives+-+Millett.pdf
14:19 < heath> wheee
14:19 < kanzure> oh i mean decodeURIComponent
14:19 < heath> also on that site which is related...
14:19 < heath> http://www.unog.ch/80256EE600585943/(httpPages)/98DD55F8A0EF259DC12574B200461162?OpenDocument
14:19 < heath> .title
14:19 < yoleaux> The United Nations in the Heart of Europe | Disarmament | BWC MX 2008 Synthetic Biology Seminar
14:19 < kanzure> console.log(decodeURIComponent("http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf"))
14:19 < kanzure> http://unog.ch/80256EDD006B8954/(httpAssets)/2C8C2466169C45F5C1257770004E66B7/$file/International+perspectives+-+Millett.pdf
14:22 < heath> https://www.youtube.com/watch?v=x5yPkxCLads
14:22 < heath> .title
14:22 < yoleaux> The PCR Song - YouTube
14:24 < fenn> maybe yoleaux should just .title any bare youtube links on their own line
14:26 < fenn> .decode "http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf"
14:26 < yoleaux> "http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf"
14:35 < fenn> .bruce
14:35 < heath> .willis
14:37 < fenn> http://tumbolia.appspot.com/fact/http://geekz.co.uk/schneierfacts/
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15:08 < brownies> .meow
15:08 < yoleaux> http://moar.edgecats.net/cats/tumblr_m3p7m9DutI1rows1to1_250.gif
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19:12 < jonathan__> paging max
19:16 < jonathan__> "Look at this amazing FOOD this 3D printer can make! NOTE: Food not edible."
19:20 < yashgaroth> ha
19:25 < yashgaroth> oh 3d printing, what won't you try to do badly and then hype the fuck out of it
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20:08 < jonathan__> the new black sabbath album is pretty sweet
20:34 < heath> ellen got back with me
20:34 < heath> http://igem.org/wiki/images/e/ec/UNICRI-synNanobio-final-2-public.pdf
20:34 < heath> .title
20:34 < heath> that's the link she gave
20:34 < yoleaux> heath: Sorry, that doesn't appear to be an HTML page.
20:38 < heath> """the part about fostering a culture of shared responsibility is in the summary on page 10 under "Response Options"."""
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--- Log closed Sun Jun 23 00:00:04 2013