--- Log opened Sat Aug 03 00:00:44 2013
00:14 < heath> .title https://www.youtube.com/watch?v=xs1u3dIRcyY
00:14 < yoleaux> Dr. Laura Landweber - "Epigenetics: Rewriting the information in DNA" - YouTube
00:14 < heath> fenn: that would be the better video
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02:05 < strangewarp> http://www.dhs.gov/how-we-fuck-you-in-your-ass - hmm
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06:54 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by george church and the NRA | http://gnusha.org/logs http://diyhpl.us/wiki http://groups.google.com/group/diybio | banned by the Federal Death Administration | official paperbot fan club
06:54 -!- Topic set by kanzure [~kanzure@131.252.130.248] [Sat Mar 23 20:40:45 2013]
06:54 [Users ##hplusroadmap]
06:54 [@bkero       ] [ chris_99      ] [ heath        ] [ nully         ] [ Shehrazad_ ] [ underscor ] 
06:54 [@kanzure     ] [ cogitokat     ] [ HEx1         ] [ oblique       ] [ sivoais    ] [ upgrayeddd] 
06:54 [@nmz787      ] [ Coornail      ] [ ivan`        ] [ paperbot      ] [ smeaaagle  ] [ Urchin    ] 
06:54 [ abetusk     ] [ devrandom     ] [ jrayhawk_    ] [ ParahSailin   ] [ spresser   ] [ yoleaux   ] 
06:54 [ AlonzoTG    ] [ EnLilaSko     ] [ juri_        ] [ ParaSa1lin    ] [ strangewarp] [ yorick    ] 
06:54 [ archels     ] [ fenn          ] [ juul         ] [ pasky         ] [ streety    ] [ Zhwazi    ] 
06:54 [ AshleyWaffle] [ fredox        ] [ lichen       ] [ phryk         ] [ superkuh   ] [ zubaz     ] 
06:54 [ audy        ] [ gnusha        ] [ lupfantomo   ] [ randallagordon] [ Thomas42_  ] 
06:54 [ balrog      ] [ gnusha_       ] [ monkeynipples] [ rigel         ] [ ThomasEgi  ] 
06:54 [ brownies    ] [ gradstudentbot] [ nsh          ] [ ryankarason   ] [ Thorbinator] 
06:54 [ Burninate   ] [ Guest10542    ] [ nuba_        ] [ saurik        ] [ ua         ] 
06:54 -!- Irssi: ##hplusroadmap: Total of 62 nicks [3 ops, 0 halfops, 0 voices, 59 normal]
06:54 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010
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07:49 <@kanzure> bleep boop
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07:54 < fredox> that reminds me
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08:25 <@kanzure> fredox: ?
08:25 <@kanzure> yashgaroth: sup
08:26 < yashgaroth> apparently the guy who brought in a bunch of equipment to the lab has been asked to remove it by assaydepot guy
08:26 < fredox> i never grabbed the last venture bros
08:27 < ParaSa1lin> brock dies
08:29 <@kanzure> brock dies? aw fuck.
08:29 <@kanzure> oh that's right, the later seasons have some other voice actor
08:39 <@kanzure> yashgaroth: so they just didn't want the equipment?
08:39 < yashgaroth> I doubt it, since it was over half of our stuff...maybe some sort of personal falling-out that everyone else has to deal with
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08:40 < yashgaroth> admittedly 90% of his stuff was obsolete and/or broken but still, the one working -80 and floor centrifuge and power supplies and such
08:40 < ParaSa1lin> how was nmz787's spec going anyway
08:40 < gradstudentbot> Where are the hot plates?
08:40 < ParaSa1lin> thats a project with some prospect
08:41 <@kanzure> ParahSailin: i think he's still busy trying to find someone who knows optics things
08:51 < ParaSa1lin> put out a craigslist ad for one
08:51 < ParaSa1lin> or buy one of the chinese knockoffs and reverse engineer a reverse engineered nanodrop
08:54 < ParaSa1lin> id be willing to pay a little extra to see it complete sooner
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09:13 <@kanzure> yeah i don't remember what his holdup is. i thought it was optics, like sourcing a lense or something?
09:14 <@kanzure> http://tequals0.wordpress.com/2013/08/03/the-gude-to-diy-gel-illumination/
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09:45 <@kanzure> oh.. hello me, https://gist.github.com/kanzure/5558267
09:45 <@kanzure> i wish i would have also published commit-cleaner.py because it's currently on another machine
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10:41 < abetusk> Did you see the guideline change on Kickstarter?  "Projects cannot offer genetically modified organisms as a reward."  http://www.kickstarter.com/help/guidelines
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10:50 <@kanzure> yep
11:06 < abetusk> I think it has a chilling effect on future projects.  Very short sighted
11:08 <@kanzure> it doesn't matter, that's just kickstarter
11:13 < abetusk> it does matter.  Biology projects will benefit from crowd funding, of which Kickstarter is a major player
11:14 <@kanzure> kickstarter isn't doing the crowdfunding
11:14 <@kanzure> i think you have it backwards?
11:15 < cogitokat> How do I respond to a message that was sent before I joined the mailing list?
11:15 < abetusk> they facilitate crowd funding.  They provide the infrastructure for crowd funding projects
11:16 <@kanzure> abetusk: i think you can just scrape the list of people who donated to biocurious/glowing plant/whatever and just email them directly. problem solved.
11:17 < abetusk> kanzure, I'm not so sure.  That list is certainly important, but I think there is a large market for people with low involvement in the DIY bio community that would like to donate/fund/invest in such projects
11:17 <@kanzure> it's not investing
11:17 < yashgaroth> kickstarter projects have all the donors' emails listed publicly?
11:17 <@kanzure> no, but i have their emails because i stalked everyone
11:17 < yashgaroth> haha fair enough
11:17 <@kanzure> and i have a database of people who have monetarily contributed to those projects
11:17 <@kanzure> (because fuck kickstarter)
11:17 < abetusk> it depends on how liberal your definition of investing is.
11:18 < abetusk> When I fund open source projects, I consider it an investment
11:18 <@kanzure> maybe you should stick with SEC definitions
11:18 < abetusk> fair enough
11:18 <@kanzure> heh
11:18 <@kanzure> anyway, i think if those people exist then they would also exist outside of kickstarter.com
11:19 < abetusk> And you could certainly go to some other crowd source funding sight, create your own, etc., but Kickstarter is one of the largest
11:19 < abetusk> *site
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12:12 <@nmz787> ParaSa1lin: being lazy on layout... circuit schematic is done, started layout.. need to get through it once and have a mixed-signal guru look it over
12:12 <@nmz787> optics are simple, if the chinese company didn't rip me off for a slug of random plastic with lines on it, it should be plug n chug (or play)
12:13 <@nmz787> (simple was a huge realization, after I learned how complex it all really is)
12:13 <@nmz787> simple but it has to be done right
12:13 <@nmz787> figuring out what is right was hard
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12:41 < fredox> "Taq works best at a G/C content of no more than 50%. Addition of extra Mg2+ and optimization will usually work for up to 60%. For even higher G/C templates, Taq is unreliable." -> OpenTaq = 59% gc
12:43 <@kanzure> what is opentaq?
12:43 <@kanzure> do you mean one of the unpatented taq variants?
12:47 < yashgaroth> do you mean the gene sequence for the opentaq protein is 59% gc? because that's irrelevant to what sequences the protein will amplify
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12:54 < fredox> sequence is open source -> protein is sold. Idea was use sequence as test run for synth, then use to make your own protein. kinda ironic that it dosen't like amping its own source
12:55 < fredox> kanzure: http://www.openbiotech.com/v/vspfiles/assets/images/popentaq%20datasheet.pdf
13:03 <@kanzure> i'm confused about what you are complaining about
13:03 <@kanzure> can you state your complaint in the form of heckling?
13:04 <@kanzure> also, i forgot that john called his thing opentaq. that's sort of a douche move but whatever. from henceforth we shall refer to that as schloendorn taq.
13:04 < fredox> Hey Taq, why dont you go amplify yourself? Oh thats right you cant hahaha
13:04 <@kanzure> unreliable doesn't mean it never works
13:06 < fredox> yeah but i dont want use something that apparently unrelaible as a test for prototype function verifaction
13:06 < fredox> fication even
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13:21 <@kanzure> eh, you could just sample a few thousand different polymerases i guess
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13:22 <@kanzure> try searching through ncbi for polymerases that have an even distribution of nucleotides
13:23 < fredox> I've just beeen looking around for a good simple target to test in the kb range. seemed fortuitous to notice that
13:24 < fredox> but yeah i'll have a look
13:24 <@kanzure> most people use gfp because they can force an ecoli to express it
13:25 < yashgaroth> what about, like, the rest of the opentaq plasmid? looks like it's about 50/50 gc
13:25 <@kanzure> most oligo synthesizers can't synthesize gfp
13:29 <@kanzure> (without forcing you to manually ligate oligos together)
13:29 <@kanzure> but if you're okay with ligation then you should be okay with synthesizing 25 bp fragments at a time
13:29 <@kanzure> so..
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16:09 < anannie> Okay, so for week 1 I created a three wheeled drag racer... (I didn't have a differential )
16:09 < anannie> http://imgur.com/a/IjHXm
16:10 <@kanzure> where are the leocad files
16:11 < anannie> kanzure: Leocad doesn't run on my tiny netbook I'm afraid
16:11 <@kanzure> because of ram?
16:11 < anannie> Yes and the lousy atom CPU
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16:12 <@kanzure> not because of architecture?
16:13 < anannie> ?
16:13 <@kanzure> what?
16:13 <@kanzure> do you get an error message when you try to run leocad?
16:15 < anannie> It's been a while
16:15 < anannie> I don't remember
16:17 < anannie> I'll try to do it again and I'll tell you what happens
16:17 < ThomasEgi> anannie, that thing needs propper bearings and about 300W more power output on the wheels ;)
16:18 < anannie> http://imgur.com/a/cHAN7#0 <--- corrected the photos
16:18 < anannie> ThomasEgi: Heh. I'm afraid that's the best I can do right now
16:20 < ThomasEgi> you'll grow up to play with bigger motors one day
16:23 < anannie> ThomasEgi: I already have...
16:24 < ThomasEgi> grown up or played with bigger motors ;)
16:25 < anannie> I've played with bigger motors
16:26 < anannie> and I'm still growing up, so there isn't anything I can say over there...
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16:38 < ThomasEgi> growing up ain't that important. the motors however.. are so fun :3
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17:51 <@nmz787> looks cool anannie
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19:36 < AshleyWaffle> relevant cause diy: http://www.youtube.com/watch?v=W9cK_TMLvjI
19:38 <@kanzure> .title
19:38 < yoleaux> Soda can Stirling engine - 860 rpm - YouTube
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20:37 < anannie> I've been reading the wikipedia article for stirling engines AshleyWaffle and even though I studied thermodynamics intently once upon a time, it isn't immediately obvious to me why this works
20:37 < AshleyWaffle> heh
20:40 < anannie> I mean I've read about the entire carnot cycle, but I don't grok why on earth this actually works
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22:21 < tomkinsc> paperbot: http://link.springer.com/article/10.1007/BF00028018#page-1
22:21 < paperbot> http://libgen.org/scimag2/10.1007/BF00028018.pdf
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23:30 < anannie> brownies: I didn't fancy seeing you in here :)
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--- Log closed Sun Aug 04 00:00:03 2013