--- Log opened Wed Dec 31 00:00:08 2014
00:12 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
00:17 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Ping timeout: 255 seconds]
00:17 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
00:22 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Read error: Connection reset by peer]
00:22 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
00:33 -!- juri_ [~juri@vpn166.sdf.org] has joined ##hplusroadmap
00:49 -!- delinquentme [~dingo@74.61.157.78] has quit [Ping timeout: 245 seconds]
01:08 < nmz787> kanzure: I tried bumping the quality of an example of this and got a stack size limit error from recursion http://openjscad.org/
01:09 < nmz787> too bad too, because they have a lot of API and their docs seem really really good
01:10 < nmz787> this is a demo where your code changes will update
01:10 < nmz787> http://joostn.github.io/OpenJsCad/
01:22 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 265 seconds]
01:26 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap
01:29 -!- delinquentme [~dingo@74.61.157.78] has joined ##hplusroadmap
01:35 -!- top4o [~chatzilla@93.152.162.95] has quit [Quit: ChatZilla 0.9.91.1 [Firefox 34.0.5/20141126041045]]
01:56 -!- poppingtonic [~poppingto@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer]
01:56 -!- poppingt` [~poppingto@105.230.21.134] has joined ##hplusroadmap
01:57 -!- delinquentme [~dingo@74.61.157.78] has quit [Ping timeout: 244 seconds]
02:05 -!- poppingt` is now known as poppingtonic
02:05 -!- poppingtonic [~poppingto@105.230.21.134] has quit [Changing host]
02:05 -!- poppingtonic [~poppingto@unaffiliated/poppingtonic] has joined ##hplusroadmap
02:14 -!- delinquentme [~dingo@199.116.73.50] has joined ##hplusroadmap
03:27 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Remote host closed the connection]
03:28 -!- FAMAS2 [~FAMAS@182.48.84.41] has joined ##hplusroadmap
03:32 -!- FAMAS [~FAMAS@182.48.84.41] has quit [Ping timeout: 240 seconds]
03:43 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
04:08 -!- ebowden_ [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
04:08 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Read error: Connection reset by peer]
04:08 -!- poppingtonic [~poppingto@unaffiliated/poppingtonic] has quit [Quit: ERC Version 5.3 (IRC client for Emacs)]
04:09 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
04:12 -!- poppingtonic [~poppingto@unaffiliated/poppingtonic] has joined ##hplusroadmap
04:14 -!- eudoxia [~eudoxia@r186-50-239-232.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap
04:22 -!- narwh4l [~michael@unaffiliated/thesnark] has joined ##hplusroadmap
04:30 -!- delinquentme [~dingo@199.116.73.50] has quit [Remote host closed the connection]
04:39 -!- poppingtonic [~poppingto@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer]
04:52 -!- pete4242 [~smuxi@boole.london.hackspace.org.uk] has joined ##hplusroadmap
04:55 -!- delinquentme [~dingo@199.116.73.50] has joined ##hplusroadmap
04:55 -!- FAMAS3 [~FAMAS@182.48.84.141] has joined ##hplusroadmap
04:57 -!- FAMAS2 [~FAMAS@182.48.84.41] has quit [Ping timeout: 250 seconds]
05:01 -!- eudoxia [~eudoxia@r186-50-239-232.dialup.adsl.anteldata.net.uy] has quit [Remote host closed the connection]
05:12 -!- drewbot [~cinch@ec2-54-91-138-113.compute-1.amazonaws.com] has quit [Remote host closed the connection]
05:12 -!- drewbot [~cinch@ec2-54-204-83-236.compute-1.amazonaws.com] has joined ##hplusroadmap
06:05 -!- delinquentme [~dingo@199.116.73.50] has quit [Ping timeout: 272 seconds]
06:20 -!- delinquentme [~dingo@74.61.157.78] has joined ##hplusroadmap
06:21 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-jgqejafbvzijyoti] has joined ##hplusroadmap
06:31 -!- delinquentme [~dingo@74.61.157.78] has quit [Ping timeout: 265 seconds]
06:57 -!- shannon [~shananana@unaffiliated/shannon] has quit [Quit: Leaving]
07:04 -!- Merovoth [~Merovoth@gateway/tor-sasl/merovoth] has quit [Remote host closed the connection]
07:05 < kanzure> "Bacteriophage adhering to mucus provide a non–host-derived immunity" http://www.pnas.org/content/110/26/10771.short
07:05 < kanzure> "Based on these observations, we present the bacteriophage adherence to mucus model that provides a ubiquitous, but non–host-derived, immunity applicable to mucosal surfaces. The model suggests that metazoan mucosal surfaces and phage coevolve to maintain phage adherence. This benefits the metazoan host by limiting mucosal bacteria, and benefits the phage through more frequent interactions with bacterial hosts. The relationships shown ...
07:05 < kanzure> ... here suggest a symbiotic relationship between phage and metazoan hosts that provides a previously unrecognized antimicrobial defense that actively protects mucosal surfaces."
07:07 -!- nsh [~lol@wikipedia/nsh] has quit [Read error: Connection reset by peer]
07:08 -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap
07:14 < kanzure> -_- marcus hutter http://consc.net/pics/singularity@@/aixi.jpg
07:15 < kanzure> er... this is a strange email
07:15 < kanzure> it's from "Herschel Shmuel Pinchas Yerucham Krustofsky <herschelshmuelpinchasyerucham@gmail.com>
07:16 < kanzure> er, -"
07:16 < kanzure> sent to diybio-israel@googlegroups.com
07:16 < kanzure> "I have another good friend looking for a shidduch. His name is Alfred Rosenberg and he is in his early 30's. He is a modern yeshivish guy from New York who is now living in Bnei Brak. He is a brilliant biochemist hired by the Mossad to develop an ethnic bioweapon to eliminate Arabs, liberals, and mothers-in-law.  Mr. Rosenberg is open to dating women from all backgrounds or walks of life, including divorcees and converts, even older ...
07:16 < kanzure> ... women up to age 40  If you know any young women in the parshah whom you think might be appropriate for him, please give him a call at 054-550-7796 or call me at 074-701-0411. "
07:16 < kanzure> very odd troll
07:17 < kanzure> "The Mossad, short for HaMossad leModiʿin uleTafkidim Meyuḥadim, is the national intelligence agency of Israel."
07:17 < kanzure> huh, this spam seems to be everywhere
07:22 < archels> haha, what in the hell.
07:22 < archels> have you tried calling the numbers?
07:23 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 245 seconds]
07:23 -!- ebowden_ [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Remote host closed the connection]
07:28 < kanzure> no
07:29 -!- Merovoth [~Merovoth@gateway/tor-sasl/merovoth] has joined ##hplusroadmap
07:30 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 250 seconds]
07:37 < heath> https://github.com/indrora/Atomic
07:37 < heath> Atomic: an IRC client from the ashes of yaaic
07:45 -!- sheena [~home@S0106c8be196316d1.ok.shawcable.net] has joined ##hplusroadmap
07:50 -!- FAMAS2 [~FAMAS@182.48.84.141] has joined ##hplusroadmap
07:54 -!- FAMAS3 [~FAMAS@182.48.84.141] has quit [Ping timeout: 255 seconds]
08:05 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-jgqejafbvzijyoti] has quit [Ping timeout: 244 seconds]
08:09 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-bwmbmkxcnszytcqe] has joined ##hplusroadmap
08:12 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap
08:16 < maaku> happy new year everyone
08:16 -!- narwh4l [~michael@unaffiliated/thesnark] has quit [Remote host closed the connection]
08:19 < kanzure> no time travel
08:24 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-bwmbmkxcnszytcqe] has quit [Quit: Connection closed for inactivity]
08:31 < bbrittain> maaku: you too!
08:31 < maaku> well it's 2015 where I am :P
08:32 < bbrittain> kanzure: he is a time traveler. *gasp*
08:32 < kanzure> time travel is very dangerous
08:33 < bbrittain> maaku: asia?
08:34 < maaku> bbrittain: taipei
08:35 < maaku> kanzure: I wish there was a sci fi movie that really, properly investigated time travel in a serious way
08:35 < bbrittain> I love taipei.
08:35 < maaku> Primer probably comes the closest
08:35 < maaku> bbrittain: who wouldn't? taipei has everything
08:36 < bbrittain> 台北是我的最喜欢的城市
08:36 < kanzure> there was a study recently where someone crawled the web looking for evidence of time travel
08:36 < bbrittain> and about 100x more 7/11s than you need
08:36 < kanzure> if i was better at conspiracy theories, i would spin some lies about how the web is really just a CERN project related to particle acceleration and time travel
08:37 < maaku> lies?
08:37 < maaku> :P
08:38 < bbrittain> maaku: do you live in taipei or just traveling?
08:38 < kanzure> everyone knows that the only way that time travelers are able to communicate is through crazy usenet emails from the 80s
08:38 < kanzure> because CERN was hosting a usenet node back then
08:38 < kanzure> next to their synchotron
08:40 < kanzure> maaku: have you been exposed to the "molecular biology and cells are already self-replicating nanotechnology and we should be using that instead of employing fantasies of diamondoid mechanosynthesis" line of reasoning before?
08:41 < maaku> bbrittain: eh.. both? my wife is from taipei city. we're on extended travel / remote work at the moment, staying with her aunt downtown (nearest MRT Chiang Kai‑shek Memorial Hall)
08:42 < maaku> i'll be back stateside in a few weeks though. gotta make fc15 in peurto rico in late january
08:42 < bbrittain> maaku: very nice. I imagine it's quite nice out there. Every time I've been there there has been a typhoon :/
08:42 < bbrittain> kanzure: is that a bad line of logic?
08:42 < bbrittain> that makes perfect sense to me...
08:42 < maaku> haha i've yet to experience one of those. we usually don't go during typhoon season
08:42 < maaku> feels like summer in san fransico
08:44 < maaku> kanzure: it's a great idea if what you want to do is biology
08:44 < kanzure> bbrittain: well the most reasonable objection that i've seen is "biology sucks and the protocols suck and it's not anything like engineering"
08:44 < maaku> meh who cares if it's not like engineering, so long as it gets the job done
08:44 < kanzure> maaku: hm? you can do lots of non-biology sttuff with biology. like you can make proteins to do... things.. and structured like.. things.
08:44 < maaku> but from what i can tell it doesn't get the job done
08:45 < kanzure> wait, which job in this context pls?
08:45 < bbrittain> biology? correct.
08:45 < bbrittain> :P
08:47 < maaku> kanzure: curing all diseases, ending aging, strengthening the body against mechanical failure, mind uploading. in that order.
08:48 < bbrittain> maaku: those are some hefty jobs
08:49 < bbrittain> I vaugly expect it to be easier in this order: strengthening body, ending aging, curing all diseases, mind uploading.
08:49 < kanzure> "curing all diseases" should be dead last
08:49 < bbrittain> well if we have mind uploading, would it be worth it to even cure all diseases?
08:50 < kanzure> i would be pretty happy with "a general ability and good reasons to expect anyone to be able to cure biological disease" rather than "some sort of centralized attempt at disease erradication"
08:52 < kanzure> well anyway, hm
08:52 < kanzure> actually, i might place mind uploading as easier than ending aging
08:54 < bbrittain> kanzure: I think thats crazy, the brain is obscenly complicated
08:55 < bbrittain> maaku: are you saying you think that biology, just doesn't get the job done?
08:55 < bbrittain> IIRC, you worked at transcriptic; did that influence that opinion?
08:55 < kanzure> generally i think it's probably correct to say things like "it is harder to do those things with biology than it would be with generic molecular nanotechnology"
08:56 < maaku> sorry was afk
08:56 < maaku> bbrittain: no, transcript is awesome and does good work
08:57 < bbrittain> good to hear, they are seeming more and more useful
08:57 < kanzure> maaku: i don't know if i have indoctrinated you into the cult of diybio yet, but the lab centralization trends are worrisome
08:57 < kanzure> on the one han, i think a generic biology lab api is a good thing
08:57 < kanzure> on the other hand, it's already hard enough for me to acquire good lab equipment
08:58 < maaku> the ordering of those tasks has to do with singularity related concerns, somewhat unrelated (i maybe shouldn't have specified order)
08:58 < kanzure> centralization would tend to make acquiring equipment even more difficult
08:58 < maaku> but taking them in the order listed...
09:00 < maaku> cure all diseases: we have diseases for which we know the mechanism, or we know how to identify affected cells (e.g. cancers). yet these diseases remain uncured
09:00 < maaku> this is embarassing. the problem is that the tools available to us from biology don't provide the necessary functionality to destroy the disease
09:01 < maaku> we should be able to e.g. scan the genome of every cell for certain virus dna, and remove if found + carry out some other procedures, or identify cancerous cells or viruses or bacteria and destroy in place
09:02 < kanzure> cancer is not a good example because of the million different mechanisms
09:02 < kanzure> certain subtypes of cancer are excellent examples, though
09:03 < maaku> kanzure: no it's a perfect example because there are specific cancers which we can identify (you do a biopsy and say "these cells are cancer of type XYZ"), and yet you still die of the damn disease
09:03 < kanzure> we do have ways to destroy certain types of cancers based on personalized medicine (e.g. custom viruses) but again this does not work for all forms of cancer
09:04 -!- sheena [~home@S0106c8be196316d1.ok.shawcable.net] has quit [Ping timeout: 240 seconds]
09:04 < bbrittain> I tend to agree that that is embarassing though
09:04 < maaku> why can't we? oh yeah, biology is basically doing chemistry with boxing gloves (protiens), applied shotgun style to the body. even personalized medicine fits this category
09:06 < bbrittain> maaku: oh, I don't think thats quite fair
09:06 < bbrittain> shotgun, yes
09:06 < bbrittain> but that can be refined
09:06 < bbrittain> "personalized medicine" is a joke right now
09:07 -!- FAMAS2 is now known as FAMAS
09:07 < maaku> i'm not saying its useless. I'm just saying it'd be so much better if we could remove the gloves
09:08 < bbrittain> the proteins? elaborate.
09:10 < maaku> no just metaphorically. having the ability to e.g. destructively disassemble a cell, recording the exact composition, structure, and position of every molecule found within
09:11 < maaku> or the ability to selectively inject a protien, rna, or other package into any specific cell
09:12 < maaku> or the ability to "map-reduce" operations on the body -- identify cells by arbitrary constraint X, perform arbitrary operation Y
09:12 < maaku> that would go pretty damn far towards curing all diseases
09:13 < maaku> and is the sort of thing provided by Freitas-like nanomedecine built with diamandoid tools, not biology
09:14 < maaku> similarly for biology -- if you can do atomically precise studies on representative cell types and tissue from a very large population, identifying the causes of ageing is basically an exericise in statistical analysis
09:16 < maaku> 3rd item, i would like to upgrade the human body such that it is able to disperse the energy of an high energy bullet, survive catestrophic breakup of an airliner, survive without oxygen for 24 hours, and probably a host of other things requried to make sure accidental death no longer occurs
09:18 < maaku> for curing disease, ending ageing, and radical body upgrades, wet biology doesn't seem to cut it for the above mentioned reasons. and I have yet to hear of a way to achieve mind uploading via biology only
09:19 < bbrittain> haven't you seen avatar? (I'll have more serious responses onec I'm done making this coffee)
09:21 < maaku> so kanzure, I respect the people who say that we should improve on biotech rather than diamandoid nanotechnology. they are often solving real problems today and for that deserve my respect.
09:21 < maaku> but i do claim they're not dreaming big enough :)
09:21 < fenn> fwiw drexler takes the position we should work on biotech first
09:23 < maaku> fenn: I think that is a misrepresentation. drexler is working on bootstrapping diamandoid mechanosythesis via engineered protien structures
09:23 < kanzure> that sounds like biotech to me
09:24 < maaku> kanzure: maybe. another way of looking at it is traditional engineering, just using DNA as scaffolds, lipids as container walls, etc. the structures I've seen very clearly look engineered
09:26 < fenn> we also build houses out of wood :\
09:28 < fenn> http://metamodern.com/2009/03/19/a-high-performance-polymer-for-nanosytems-engineering/
09:30 < kanzure> in the absence of working molecular nanotechnology, biotech seems like a good way to get stuff done
09:30 < maaku> kanzure: right, well my response is "sounds like someone needs to be working on molecular nanotechnology!"
09:31 < fenn> "to my astonishment the field has languished on the sidelines, and often isn’t thought of as being a nanotechnology."
09:31 < maaku> i'm okay with working on long-term, high-payoff projects. indeed i tend to gavitate towards those
09:31 < fenn> [the field of rational protein design]
09:32 < bbrittain> I think that none of these are mutually incompatable. Ideally we would reach a point in biotech where iff a diamonoid mechanism works better, it could just be incorporated, no?
09:33 < bbrittain> My bigest problem with biotech is how every single thing just increases complexity, not decreases it. :/
09:33 < fenn> no, not if your technology is completely modular and resuable components, as biology tends to do
09:34 < fenn> vs covalent one-off structures made of pure carbon or whatever
09:34 < bbrittain> fenn: sorry, did you just say that biology is completely modular?!
09:34 < fenn> yes
09:34 < fenn> on a chemical level at least
09:35 < maaku> fenn: right, but notice how he talks about joining peptide chains together into atomically precise configurations, laments the lack of CAD-like tools, and stresses the differences from biology (his words)
09:35 < bbrittain> I mean, thats like saying a processor is modular because electricity.
09:35 < maaku> he's talking about atomically precise ceramics, not meat
09:35 < kanzure> he's talking about protein
09:35 < fenn> ceramics is incorrect, but i agree
09:36 < fenn> we don't have a word for this material probably
09:36 < kanzure> the other day i viewed the images of all rcsb pdb proteins with more than 300 residues (tens of thousands of proteins)
09:36 < maaku> Right sorry, that's obviously not correct. And yeah I don't konw what the word would be for that material...
09:37 < kanzure> out of all of those, here are some with interesting and novel shapes http://diyhpl.us/wiki/dna/projects/#proteins
09:37 < kanzure> however, there's a few hundred thousand proteins with less than 300 residues
09:37 < fenn> .c 300^20
09:37 < yoleaux> 300²⁰ = 34867844010000000000000000000000000000000000000000
09:37 < fenn> or is it 20^300
09:38 < bbrittain> .c 20^300
09:38 < yoleaux> 20³⁰⁰ = 2037035976334486086268445688409378161051468393665936250636140449354381299763336706183397376000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000 ≈ 2.037035976334486
09:38 < bbrittain> :D
09:39 < kanzure> also the the enzymatic therapy article on wikipedia is pretty hilarious ("just inject the enzymes straight into the blood what's the worst that can happen")
09:40 < maaku> bbrittain: yes you could replace something bio with something diamandoid. but that's missing the point that diamandoid tools cannot ever exist in the bio world
09:40 < bbrittain> anyways, maaku I would like to see more engineering in biology. I think that biology can be used in ending aging/curing diseases. helpful/core to strengthening body. and not useful to mind uploading, at least in any form we currently recognize as biology
09:40 < kanzure> there are definitely ways to use biology to help with mind uploading
09:40 < kanzure> like antibody tagging
09:42 < fenn> or protozoa threading fiberoptics through the brain
09:42 < kanzure> or expression of certain signals
09:43 < kanzure> and the glorious technique of in utero electroporation
09:45 < kanzure> i wonder if anyone has done radioisotope tracing of mouse learning, e.g. inject radioisotopes straight into blood, train the critter on some task, "sacrifice" four hours later, examine incorporation of radioisotopes into molecular structures
09:46 < kanzure> ah right, one of the claims is that existing structures may be sufficient without incorporation of matter for long-term storage, nevermind
09:47 < fenn> that gets you fMRI data at best
09:47 < kanzure> "“Something I’d like to do, which I think many of our engineers will be hearing this in real-time, is have the charge connector plug itself in. Like an articulating, like sort of a snake, like Metal Gear Solid or something.”" - my bro elon
09:47 < kanzure> nah, once you sacrifice the critter you can scan brain slices and look more closely
09:48 < maaku> bbrittain: I don't see them as contradictory paths. what I hope to see accomplished with molecular nanotechnology is the creation of tools that help medical professionals cure diseases
09:49 < superkuh> paperbot: http://www.nature.com/nmat/journal/vaop/ncurrent/full/nmat4164.html
09:49 < paperbot> http://libgen.org/scimag/get.php?doi=10.1038%2Fnmat4164
09:49 < kanzure> .title http://www.nature.com/nmat/journal/vaop/ncurrent/full/nmat4164.html
09:49 < yoleaux> Soft 3D acoustic metamaterial with negative index : Nature Materials : Nature Publishing Group
09:49 < superkuh> Thinking lenses for ultrasonic brain stimulation.
09:49 < fenn> aka flubber
09:50 < superkuh> Up to 500 KHz in that paper.
09:51 < maaku> the tension comes from the fact that atomically precise nanotechnology is a revolutionary advancement, not evolutionary.
09:52 < maaku> from the biologist's perspective, the nanotechnologists provide nothing useful for 15 - 20 years, until their "nanofactory" is finished and suddenly massive new capabilities are possible
09:53 < kanzure> superkuh: so you would do single-use metamaterials for specific stimulation/regional dosages? take off metamaterial and switch to a different cap for another round of differently-located stimulation?
09:55 < superkuh> I'm still trying to figure out what Mie resonators are, but yeah, something like that.
10:30 -!- eudoxia [~eudoxia@r179-25-152-29.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap
10:42 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Ex-Chat]
10:56 -!- FAMAS [~FAMAS@182.48.84.141] has quit [Ping timeout: 255 seconds]
11:01 < kanzure> nmz787: another option is you could try python-brlad
11:01 < kanzure> er
11:01 < kanzure> python-brlcad
11:01 < nmz787> diybio israel, huh... never heard of that before.... that spam sounds exactly appropriate for that list though, oddly
11:01 < kanzure> that spam is elsewhere on the internet, it's just a bot
11:02 < nmz787> dear spambot, the FBI is now watching you
11:02 < nmz787> fuck, if the FBI connects us with spambots
11:02 < nmz787> 'there are clearly maligned intentions in the eastern diybio community, bring biocurious to the ground bos'
11:03 < nmz787> boys*
11:03 < nmz787> kanzure: I thought there was tons of stuff missing from brl-cad
11:03 < nmz787> or python-brlcad
11:05 < nmz787> so bluetooth 4.0 (BLE) is supported on Android 4.3+ and also iOS with app-store authentication (as opposed to pre BLE requiring a hardware ID chip on your custom hardware)
11:05  * nmz787 designed some BLE system stuff the last few days for automotive trailer lights testing from a phone
11:07 < nmz787> it's too bad the $15 rfduino didn't have 16 pins instead of only 8 (since I didn't want to deal with muxers), I ended up going with an nRF8001 and arduino mini
11:28 < kanzure> .wik rfduino
11:28 < yoleaux> kanzure: Sorry, I couldn't find article.
11:29 < kanzure> .wik bladerf
11:29 < yoleaux> "This article provides a list of commercially available software-defined radio receivers." — http://en.wikipedia.org/wiki/List_of_software-defined_radios
11:42 < bbrittain> bladerf is cool
11:42 < bbrittain> I have a myraidrf coming
11:52 < bbrittain> kanzure: you are obsessed with ribosomes, right?
12:00 < eudoxia> ah i see from the logs that kanzure has given up on mechanosynthesis
12:00 < eudoxia> well that's a shame i guess i gotta grow up now too
12:22 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has joined ##hplusroadmap
12:41 < kanzure> eudoxia: i never say i gave up
12:41 < kanzure> eudoxia: that's not fair
12:42 < kanzure> bbrittain: no, i think it would be more fair to say i am obsessed with polymerases http://diyhpl.us/~bryan/papers2/polymerase/
12:43 < bbrittain> "I lost this page once with a crash in Opera"
12:43 < bbrittain> found your problem
12:43 < bbrittain> Opera
12:43 < kanzure> 400 tabs
12:43 < bbrittain> nah, just Opera
12:43 < kanzure> none of the other browsers can really do that these days
12:44 < kanzure> even with my 32 GB of RAM
12:44 < bbrittain> well, have you considered you may have a tab problem?
12:44 < kanzure> yes, i decided i don't
12:46 < kanzure> the main reason why i like polymerase is because of the idea of direct control over its enzymatic synthesis of dna
12:46 < kanzure> or rna, i don't really care
12:46 < kanzure> bbrittain: http://diyhpl.us/~bryan/papers2/polymerase/roadmap.pdf
12:47 < eudoxia> well that's terse
12:47 < kanzure> "we're working on it"
12:48 < kanzure> "not much progress, guys"
12:49 < kanzure> hmm i should remember to write stuff in there
12:51 < kanzure> there were a handful of good ideas sent to the mailing list
12:51 < kanzure> so they just need to be written up in a document form
13:02 -!- delinquentme [~dingo@74.61.157.78] has joined ##hplusroadmap
13:10 -!- shannon [~shananana@unaffiliated/shannon] has joined ##hplusroadmap
13:12 < kragen> BLE is sounding increasingly awesome
13:20 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has quit [Ping timeout: 250 seconds]
13:21 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has joined ##hplusroadmap
14:04 -!- Boscop [~me@unaffiliated/boscop] has quit [Quit: Boscop]
14:04 -!- Boscop [me@178.73.219.153] has joined ##hplusroadmap
14:04 -!- Boscop [me@178.73.219.153] has quit [Changing host]
14:04 -!- Boscop [me@unaffiliated/boscop] has joined ##hplusroadmap
14:07 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap
14:08 -!- eudoxia [~eudoxia@r179-25-152-29.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving]
14:27 -!- sivoais [~zaki@unaffiliated/sivoais] has quit [Ping timeout: 258 seconds]
14:27 -!- gnusha [~gnusha@unaffiliated/kanzure/bot/gnusha] has quit [Ping timeout: 258 seconds]
14:35 -!- gnusha [~gnusha@unaffiliated/kanzure/bot/gnusha] has joined ##hplusroadmap
14:35 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by george church and the NRA, banned by the Federal Death Administration (4 times) | this channel is LOGGED: http://gnusha.org/logs | http://diyhpl.us/wiki
14:35 -!- Topic set by kanzure [~kanzure@unaffiliated/kanzure] [Sat Dec 27 16:56:22 2014]
14:35 [Users ##hplusroadmap]
14:35 [ altersid_] [ cluckj       ] [ dvorkbjel] [ kragen     ] [ Qfwfq       ] [ superkuh ] 
14:35 [ andytoshi] [ comma8       ] [ fenn     ] [ maaku      ] [ rigel       ] [ ThomasEgi] 
14:35 [ archels  ] [ crescendo    ] [ gnusha   ] [ Merovoth   ] [ rk[ca]      ] [ thundara ] 
14:35 [ augur_   ] [ cuba_        ] [ heath    ] [ nArkos_    ] [ saurik      ] [ TMA      ] 
14:35 [ balrog   ] [ Daeken       ] [ helleshin] [ night      ] [ shannon     ] [ Urchin   ] 
14:35 [ bbrittain] [ delinquentme ] [ HEx2     ] [ nmz787     ] [ shubhamgoyal] [ Viper168 ] 
14:35 [ bkero    ] [ docl         ] [ ivan`    ] [ nsh        ] [ sivoais_    ] [ Vutral   ] 
14:35 [ blueskin ] [ DonnchaC_    ] [ JonTitor ] [ paperbot   ] [ smeaaagle   ] [ yoleaux  ] 
14:35 [ BobaMa   ] [ dpk          ] [ jrayhawk_] [ ParahSailin] [ soylentbomb ] [ yorick   ] 
14:35 [ Boscop   ] [ drazak       ] [ juri_    ] [ pasky      ] [ strages     ] 
14:35 [ Burn_    ] [ drewbot      ] [ juul     ] [ pete4242   ] [ strangewarp_] 
14:35 [ catern   ] [ DumpsterD1ver] [ kanzure  ] [ poohbear   ] [ streety     ] 
14:35 -!- Irssi: ##hplusroadmap: Total of 69 nicks [0 ops, 0 halfops, 0 voices, 69 normal]
14:35 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010
14:35 -!- Irssi: Join to ##hplusroadmap was synced in 12 secs
14:35 -!- kenju254 [~kenju254@static-41-242-0-196.ips.angani.co] has joined ##hplusroadmap
14:36 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap
14:42 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Quit: Colloquy for iPhone - http://colloquy.mobi]
14:49 -!- yashgaroth [~ffffff@2606:6000:cb85:6a00:50de:f53d:6d4e:517e] has joined ##hplusroadmap
14:49 -!- EnabrinTain [sid11525@gateway/web/irccloud.com/x-lrrasgdkprrbgboc] has joined ##hplusroadmap
14:49 -!- nickjohnson [sid789@gateway/web/irccloud.com/x-kslibgwomlscxain] has joined ##hplusroadmap
14:56 < kanzure> hi EnabrinTain
15:02 < delinquentme> kanzure, opinion:
15:03 < delinquentme> found a quiet fail in a codebase submitted by a googler ... was first to find it + report
15:03 < delinquentme> worth mentioning ?
15:03 < kanzure> er, why not report it?
15:05 < delinquentme> already did
15:05 < delinquentme> should I throw that in as a bullet point on the resume?
15:05 < delinquentme> perhaps I need a bugs reported section of the resume
15:05 < kanzure> oh, well i'd have to see your resume to know that
15:07 < delinquentme> oh! you've not seen my resumes?
15:07 < delinquentme> https://gist.github.com/carlcrott/4480bf5f522c178a4c22
15:07 < delinquentme> gist.github.com/carlcrott/9172903
15:07 < delinquentme> one is the white-washed version
15:08 < kanzure> move your experience section to the top
15:08 < kanzure> everything else is irrelevant
15:09 < kanzure> you have at least 8 years of experience listed, you're an old fart at this point
15:11 < delinquentme> and IDK if this dudes title is anything impressive: https://www.linkedin.com/in/erjohnso
15:12 < delinquentme> also im pissed. im not a 1% er
15:12 < delinquentme> im a 5%er
15:14 < delinquentme> Oh and whats also awesome is 3drobotics is still after me :D
15:21 < kanzure> maaybe keep your education section, but honestly nobody should care
15:25  * EnabrinTain waves at kanzure
15:26 < EnabrinTain> Happy Year Everyone
15:30  * juri_ waves.
15:42 < delinquentme> kanzure, always better to highlight best aspects
15:42 < delinquentme> marketing is ehhhh
15:42 < delinquentme> my projects speak way louder
15:43 < delinquentme> https://github.com/apache/libcloud/commit/95ae5beea125797ba3bd07028af1dbbd65dc2265#commitcomment-9131116
15:48 -!- yashgaroth [~ffffff@2606:6000:cb85:6a00:50de:f53d:6d4e:517e] has quit [Ping timeout: 265 seconds]
15:56 < delinquentme> kanzure, if we wanted to blow money for NYE
15:56 < delinquentme> what would we be doing
15:56 < kanzure> NYE?
16:05 < delinquentme> you think so?
16:05 < delinquentme> Ohh sorry. new years eve
16:05 < delinquentme> tonight
16:05 < delinquentme> sorry I thought you  meant NYC
16:05 < delinquentme> idk if thats our style
16:26 < cluckj> booze is traditional
16:29 < cluckj> tiny party hats for your cats is non-traditional
16:31 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has quit [Ping timeout: 264 seconds]
16:32 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has joined ##hplusroadmap
16:35 -!- poohbear is now known as codependentbear
16:36 -!- codependentbear is now known as poohbear
16:39 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
16:41 -!- Vutral [~ss@mirbsd/special/Vutral] has quit [Ping timeout: 255 seconds]
16:48 -!- Vutral [~ss@mirbsd/special/Vutral] has joined ##hplusroadmap
16:48 -!- pete4242 [~smuxi@boole.london.hackspace.org.uk] has quit [Remote host closed the connection]
16:50 -!- dvorkbjel [~viskestel@li607-220.members.linode.com] has quit [Remote host closed the connection]
16:55 -!- dvorkbjel [~viskestel@li607-220.members.linode.com] has joined ##hplusroadmap
17:03 -!- yashgaroth [~ffffff@2606:6000:cb85:6a00:50de:f53d:6d4e:517e] has joined ##hplusroadmap
17:08 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Ping timeout: 265 seconds]
17:13 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
17:17 -!- poppingtonic [~poppingto@unaffiliated/poppingtonic] has joined ##hplusroadmap
17:24 -!- yashgaroth [~ffffff@2606:6000:cb85:6a00:50de:f53d:6d4e:517e] has quit [Ping timeout: 272 seconds]
17:30 -!- yashgaroth [~ffffff@2606:6000:cb85:6a00:50de:f53d:6d4e:517e] has joined ##hplusroadmap
17:40 -!- sivoais_ is now known as sivoais
17:40 -!- sivoais [~zaki@199.19.225.239] has quit [Changing host]
17:40 -!- sivoais [~zaki@unaffiliated/sivoais] has joined ##hplusroadmap
17:43 < kanzure> hm
18:04 -!- shanabot [~shananana@CPE788df7e91f61-CM788df7e91f60.cpe.net.cable.rogers.com] has joined ##hplusroadmap
18:05 -!- shanabot [~shananana@CPE788df7e91f61-CM788df7e91f60.cpe.net.cable.rogers.com] has quit [Read error: Connection reset by peer]
18:07 -!- shannon [~shananana@unaffiliated/shannon] has quit [Ping timeout: 255 seconds]
18:13 -!- gene_hacker [~chatzilla@pool-173-57-37-204.dllstx.fios.verizon.net] has joined ##hplusroadmap
18:19 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has quit [Ping timeout: 272 seconds]
18:21 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has joined ##hplusroadmap
18:21 -!- HEx2 [~HEx@hexwab.plus.com] has quit [Remote host closed the connection]
18:21 < kanzure> gene_hacker: good pics on page 69 http://diyhpl.us/~bryan/papers2/neuro/Identification%20and%20functional%20analysis%20of%20Trnp1%20-%20a%20novel%20DNA%20associated%20protein%20with%20a%20key%20role%20in%20neurogenesis.pdf
18:22 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap
18:22 < kanzure> HEx1: https://groups.google.com/d/msg/enzymaticsynthesis/3YEEv0OULo0/zJZPETWDbMIJ
18:23 < kanzure> actually that link is prolly good for gene_hacker too
18:23 < HEx1> kanzure: yes, replying's been on my list of thing to do for several days now
18:23 < HEx1> +s
18:23 < kanzure> ah
18:23 < kanzure> so the system works!
18:23 < kanzure> sort of
18:24 < HEx1> yeah, I just suck is all
18:25 < kanzure> any other ideas bouncing around for dna/peptide synthesis?
18:25 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has quit [Ping timeout: 245 seconds]
18:26 < HEx1> synopsis: maybe it would be an improvement over existing chemical peptide synthesis methods if you could add a dozen or so (up to 19 I guess) tRNAs in a single step. might get better yields that way
18:26 < kanzure> oh, how would you choose which tRNA gets used though?
18:27 < kanzure> oh, you mean some in-solution capping/decapping solution that can discriminate against ~20 different tRNAs..... hmm.
18:27 < kanzure> i'm not sure we have any cappers that are that good
18:27 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has joined ##hplusroadmap
18:29 < HEx1> not quite. have mRNA with each triplet once each, repeatedly. then have a buttload of tRNAs loaded with the aminos you actually want, then add lots of them simultaneously
18:30 -!- ra_ [~ra@ool-457d6bbe.dyn.optonline.net] has joined ##hplusroadmap
18:30  * HEx1 reads about decapping
18:30 < kanzure> oh, that is the proposal that cathal suggested in response in that thread
18:30 < kanzure> i think
18:31 < HEx1> I thought he was proposing only two triplets, which alternate, and thus you only add one tRNA per step
18:33 < kanzure> "one tRNA" you mean one molecule?
18:33 < kanzure> i think you add millions of them, but all of the same type (same amino acid, same mRNA specificity)
18:33 < HEx1> no, one type
18:33 < kanzure> right ok
18:33 < kanzure> oh, you're right, if you have an alternating code in mRNA, then you could add two types of tRNA at a time
18:34 < kanzure> because one will go first, and the other next
18:34 < HEx1> no you couldn't, because it wouldn't stop after two, you'd get an arbitrary amount of addition
18:35 < HEx1> if you had mRNA with, say, AAAGGGAAAGGG..., you'd add GGG-tRNA with the amino you wanted, wash, add AAA-tRNA with the amino you wanted second, wash, etc.
18:35 < kanzure> oh sorry, you're right, i'm thinking of (n + 1) mRNA triplets, and then you can use n types of tRNA
18:36 < HEx1> exactly. we have 20 types of tRNA, we could add 19 at once. speedup: 19x. yield: potentially 19x, depends on ribosome efficiency (but they're pretty efficient, right?)
18:36 < kanzure> (so that when the mRNA triplet pattern starts repeating, nothing gets added)
18:37 < kanzure> i don't understand the 20 types thing you are suggesting
18:37 < kanzure> you need to be sure that the correct amino acid gets added next
18:38 < HEx1> yes, you need mRNA that has each triplet in turn
18:38 < kanzure> why though? maybe your protein doesn't need that next triplet
18:38 < kanzure> the "pattern" is indicated by which tRNAs you add during each cycle
18:39 < kanzure> and the mRNA is not custom (i mean, it's custom for this generic procedure, but not related to any particular future protein you will want to make)
18:39 < kanzure> i seem to be alternating between understanding and not understanding :)
18:40 < kanzure> based on my last message, that would mean my "n + 1" idea is wrong
18:40 < HEx1> the mapping between triplets and aminos is arbitrary. you need to be able to charge your tRNAs with whatever you want. if you can do that, and you have a complete set of 20*20=400 tRNAs then you can add 19 aminos in a single cycle by picking 19 tRNAs out of your library that match up with the 19 distinct triplets that come next in the mRNA
18:41 < HEx1> I should write a proper list reply explaining properly, when I'm actually sober
18:41 < kanzure> oh right, any future protein you want to make can be coded because you have a tRNA for each mRNA triplet you know you will encounter... so "n + 1" does work.
18:42 < kanzure> and you can even do "same amino acid at least n - 1 times in a row"
18:43 < kanzure> right, well, are there any particular immediate applications to having the ability to print large proteins
18:44 < HEx1> unsure. when I was thinking about this a couple of years ago I concluded proteins were kind of a dead end. you can't bootstrap anything from them
18:45 < kanzure> the number one constraint is coupling efficiency and yield on each nucleotide addition in dna synthesis
18:45 < kanzure> and a similar constraint in chemical peptide synthesis
18:45 < HEx1> but the fact that chemical peptide synthesis is even a thing indicates that there is interest
18:45 < kanzure> so even with gibson assembly you still encounter lots of yield issues
18:46 < kanzure> chemical peptide synthesis has a max residue count of like 70 to 100 at the most
18:46 < kanzure> (also things get built backwards, i dunno if this is a problem)
18:46 < HEx1> yeah. given that ribosomes can do 2 orders of magnitude better than that indicates that they're unlikely to be the limiting factor
18:47 < HEx1> interesting, hadn't run into Gibson assembly
18:47 < delinquentme> I think. Im gonna go grab some junk food.  and now that im no longer sleep depriveddddd
18:47 < delinquentme> program!
18:49 < kanzure> i wonder if there's any ribosome that is not made up of RNA
18:49 < HEx1> I was looking into existing cell-free expression systems to see what the current state is (obviously you couldn't do this in a cell)
18:50 < kanzure> do those kits do anything new and exciting that they weren't doing years ago?
18:50 < HEx1> AIUI ribosomes are *ancient*, and highly conserved across all forms of life
18:50 < HEx1> I don't know what they were doing years ago, I'm a relative n00b at all this
18:51 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Remote host closed the connection]
18:52 < kanzure> if there was a protein-constructed ribosome then you could use ribosomes to print out better ribosomes
18:53 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
18:53 < HEx1> but NEB's FAQ says "Synthetic tRNAs will have a hard time competing with the native tRNA pool."
18:53 < kanzure> you could also bootstrap different dna polymerases that are hard to manually collect
18:53 < kanzure> what native tRNA pool?
18:53 < kanzure> native to my test tube ?
18:54 < HEx1> presumably the one that came in the kit along with the E.coli ribosomes
18:54 < kanzure> ah
18:54 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Remote host closed the connection]
18:57 < kanzure> HEx1: another idea that i've seen recently is using enzymes in oligonucleotide synthesis and/or chemical peptide synthesis to increase coupling efficiencies and yields
19:05 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
19:06 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has quit [Ping timeout: 244 seconds]
19:07 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has joined ##hplusroadmap
19:12 < HEx1> questions: how easy is it to make custom tRNA? how stable are membrane-bound ribosomes (and the growing proteins) after repeated wash cycles?
19:13 < kanzure> both of these things are unknowns
19:13 < kanzure> there have been synthetic tRNAs that incorporate unnatural amino acids
19:13 -!- ra_ [~ra@ool-457d6bbe.dyn.optonline.net] has left ##hplusroadmap ["Leaving"]
19:17 < HEx1> theoretically tRNA is very easy to modify. the problem is that natural tRNA is also required for a cell to stay alive (modulo redundancy in the genetic code)
19:18 < HEx1> clearly I need to research what has been done so far
19:18 < kanzure> oh, this would definitely not be in vivo
19:19 < kanzure> although we could use cells to grow tRNAs :)
19:22 < HEx1> https://www.google.com/patents/US5358862 <-- hmm, people were playing with this long ago
19:23 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap
19:25 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Client Quit]
19:25 < kanzure> "Synthetic tRNAs are produced from tRNACys (AAA), tRNASer (AAA), tRNAe Ala (AAA), and tRNAi Ala (AAA) genes. Polyuridylic acid-dependent syntheses of polypeptides were carried out in vitro on E. coli ribosomes using the synthetic tRNAs."
19:29 < kanzure> nonribosomal peptide synthates could maybe synthesize genomes. but their sequences would be way larger than the entire genome (because you need like 8 amino acids per nucleotide you want to encode into the protein)
19:30 < HEx1> no. just no.
19:31 < kanzure> haha
19:31 < kanzure> i forget their name
19:31 < kanzure> nonribosomal.. something..
19:33 < kanzure> "Nonribosomal peptides are synthesized by nonribosomal peptide synthetases, which, unlike the ribosomes, are independent of messenger RNA. Each nonribosomal peptide synthetase can synthesize only one type of peptide."
19:33 < kanzure> nonribosomal peptide-synthetase (NRPS) enzymes"
19:33 < HEx1> like CCA-adding enzymes
19:33 < kanzure> "The enzymes are organized in modules that are responsible for the introduction of one additional amino acid. Each module consists of several domains with defined functions, separated by short spacer regions of about 15 amino acids."
19:34 < kanzure> "However the first fungal NRP to be found was ciclosporin. It is synthesized by a single 1.6MDa NRPS.[4] T"
19:35 < kanzure> hm can't find that on pdb
19:36 < kanzure> oh i see, ciclosporin isn't the protein
19:39 < HEx1> yeah. most things that aren't made by ribosomes *can't* be made by ribosomes. I'm sure there is a reason for the few that can
19:40 < kanzure> i believe there are some "nonribosomal peptide synthetases" that can, at the very least, incorporate amino acids, so i think it may be possible to find some that add nucleotides
19:40 < kanzure> i don't know if CCA-adding enzyme counts as a nonribosomal peptide synthetase
19:40 < kanzure> "
19:40 < kanzure> oh, my quote key didn't work both times. so no +" needed.
19:41 < HEx1> meh, categorisation doesn't matter, it is what it is
19:43 < HEx1> anyway. we should talk more when I have swapped this in a bit. meanwhile I need sleep
19:47 -!- DumpsterD1ver [~loki@e179203189.adsl.alicedsl.de] has quit [Ping timeout: 272 seconds]
19:50 -!- Viper168_ [~Viper@unaffiliated/viper168] has joined ##hplusroadmap
19:51 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 264 seconds]
19:58 < delinquentme> guhhhhh
19:58 < delinquentme> that server build
19:58 < delinquentme> walking away for 5 mins
19:58 < delinquentme> so goo
19:58 -!- FourFire [FourFire@2a02:270:2015:b00b:3c25:8736:f0b2:ee7c] has joined ##hplusroadmap
20:16 -!- ebowden [~ebowden@CPE-124-187-233-85.lns2.dav.bigpond.net.au] has quit [Remote host closed the connection]
20:21 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap
20:22 < kanzure> .title http://nar.oxfordjournals.org/content/19/20/5749.abstract
20:22 < yoleaux> A synthetic alanyl-initiator tRNA with initiator tRNA properties as determined by flourescence measurements: comparison to a synthetic alanyl-elongator tRNA
20:23 < kanzure> "Two synthetic tRNAs have been generated that can be enzymatically aminoacylated with alanine and have AAA anticodons to recognize a poly(U) template. One of the tRNAs (tRNAe Ala/AAA) is nearly identical to Escherichia coli elongator tRNAAla. The other has a sequence similar to Escherichia coli initiator tRNAMet (tRNAjAla/AAA). Although both tRNAs can be used in poly(U)-directed nonenzymatic initiation at 15 mM Mg2+ , only the elongator ...
20:23 < kanzure> ... tRNA can serve for peptide elongation and polyalanine synthesis. Only the initiator tRNA can be bound to 30S ribosomal subunits or 70S ribosomes in the presence of initiation factor 2 (IF-2) and low Mg2+ suggesting that it can function in enzymatic peptide initiation."
20:23 < kanzure> .title http://link.springer.com/protocol/10.1385%2F0-89603-397-X%3A105
20:23 < yoleaux> In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons - Springer
20:24 < kanzure> "Synthetic tRNAs—those that are transcribed in vitro from a DNA sequence that has been ligated into an appropriate plasmid—have a wide variety of applications that range from testing tRNAs for their requirements for amino-acylation (1) to providing reagents for the investigation of protein folding (2). The examples that will be described here involve specific tRNAs with altered anticodons. The construction of several of these tRNAs ...
20:24 < kanzure> ... will be given in detail as examples of the procedure applied. Also, methods of use and how to test the efficiency of these tRNAs will be reviewed."
20:24 < kanzure> related: "Exploring the limits of codon and anticodon size
20:25 -!- strangewarp_ [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Ping timeout: 272 seconds]
20:27 -!- poohbear is now known as poohyear
20:37 < kanzure> .title http://pubs.acs.org/doi/abs/10.1021/ja025872a
20:37 < yoleaux> An Error Occurred Setting Your User Cookie
20:37 < kanzure> "We report herein a new method for the aminoacylation of tRNA, using a resin-immobilized ribozyme and the cyanomethyl ester (CME) of an amino acid substrate. The oxidized form of the ribozyme was immobilized on a hydrazine resin via covalent linkage. We performed aminoacylation of tRNAs using this ribozyme-resin to isolate aminoacyl-tRNAs. The column was recycled up to 5 times without significant activity loss. Thus, our ribozyme-based ...
20:37 < kanzure> ... aminoacylation system has significant potential to be a powerful and practical technique for supplying various nonnatural aminoacyl-tRNAs for a highly efficient in vitro translation system."
20:37 < kanzure> "Aminoacyl-tRNA Synthesis by a Resin-Immobilized Ribozyme"
20:40 < kanzure> oh man i completely forgot about flexizyme stuff
20:47 < fenn> heh anderson cooper doesn't understand comedy at all
20:48 < kanzure> are they still irradiating your eyeballs geeze
20:48 < fenn> this was self inflicted... enablers
20:49 < fenn> i like to watch the new york countdown
20:49 < kanzure> https://www.youtube.com/watch?v=bPBzj90Su8A
20:49 < fenn> .title
20:49 < yoleaux> Quark They Irradiated Their Own Planet? - YouTube
20:50 -!- delinquentme_ [~dingo@74.61.157.78] has joined ##hplusroadmap
20:51 < fenn> why does he say it three times?
20:51 < kanzure> youtube has this reality distortion field called "poop"
20:51 < fenn> and it will destroy the universe
20:51 < fenn> and has destroyed the universe
20:52 -!- delinquentme [~dingo@74.61.157.78] has quit [Ping timeout: 264 seconds]
20:52 < kanzure> poop example https://www.youtube.com/watch?v=Bt7MN8f4YGk
20:53 < delinquentme_> https://www.youtube.com/watch?v=8xHXx5HARC8 fun.0
20:53 < kanzure> .title
20:53 < yoleaux> 5 Crazy Science Stunts, You Won't See At School - YouTube
20:54 < kanzure> meh
20:58 < delinquentme_> watch the second one
20:58 < delinquentme_> need liquid nitrogen
21:01 -!- FourFire [FourFire@2a02:270:2015:b00b:3c25:8736:f0b2:ee7c] has quit [Quit: Leaving]
21:03 < fenn> man this place is totally dead quiet.. no explosions no nothing
21:08 < kanzure> .title https://www.youtube.com/watch?v=UkEuIlGLZkY
21:08 < yoleaux> Darth Vader sings the Internationale - YouTube
21:11 -!- ebowden [~ebowden@CPE-124-187-233-200.lns2.dav.bigpond.net.au] has joined ##hplusroadmap
21:24 -!- rayston [~rayston@ip68-106-242-42.ph.ph.cox.net] has joined ##hplusroadmap
21:24 < gene_hacker> no explosions? then make them
21:30 < delinquentme_> omg
21:48 -!- gene_hacker [~chatzilla@pool-173-57-37-204.dllstx.fios.verizon.net] has quit [Ping timeout: 272 seconds]
21:51 < delinquentme_> also knights of sidonia is nuuuts as is gargantia on the vardious planet
22:07 -!- Beatzebub [~beatzebub@d172-218-204-36.bchsia.telus.net] has joined ##hplusroadmap
22:08 < fenn> there's nothing like an alien/clone/hermaphrodite love triangle to warm the cockles of my heart
22:17 < kanzure> this is a wonderful resource http://www.psydb.net/samples/original.php
22:18 < fenn> .title
22:18 < yoleaux> PsyDB: Psychedelic trance / psytrance samples: Original
22:18 < fenn> is this where DJs get all the cheesy sound effects
22:18 < kanzure> basically it's a reverse index of "weird quotes and samples you may have once heard many years ago in some random song while coding"
22:18 < kanzure> for example,
22:19 < kanzure> https://www.youtube.com/watch?v=g1LwIrAABVk
22:19 < fenn> right
22:19 < fenn> "the distance between insanity and genius is measured only by success"
22:24 -!- Beatzebub_ [~beatzebub@d172-218-204-36.bchsia.telus.net] has joined ##hplusroadmap
22:24 < kanzure> https://www.youtube.com/watch?v=PZL8cjCzSPs&t=4m5s
22:27 < nmz787> .title
22:27 < yoleaux> Cyclics - Movement Within the 4th Dimension - YouTube
22:27 -!- Beatzebub [~beatzebub@d172-218-204-36.bchsia.telus.net] has quit [Ping timeout: 244 seconds]
22:27 < kanzure> ugh https://www.youtube.com/watch?v=W0ADdohktbQ&t=6m55s
22:27 -!- Beatzebub [~beatzebub@d172-218-204-36.bchsia.telus.net] has joined ##hplusroadmap
22:28 < kanzure> too bad this index is incomplete.
22:28 < fenn> http://fennetic.net/irc/orbital_moebius.mp3
22:30 -!- Beatzebub_ [~beatzebub@d172-218-204-36.bchsia.telus.net] has quit [Ping timeout: 256 seconds]
22:32 < kanzure> there's this one that i can't find that literally used quotes sampled from the back to the future movies
22:33 < kanzure> usually i am pretty good at finding impossible to find things
22:33 < kanzure> but this is hard when people name their tracks stuff like "DJ x volume 5140"
22:33 < nmz787> kanzure: wasn't there some other online CAD thing that had some non-free services associated?
22:33 < kanzure> there are many online cad things
22:34 < nmz787> it was open though
22:34 < nmz787> I think
22:34 < nmz787> is that verb?
22:34 < nmz787> does it have some non-free front-end?
22:35 < fenn> are you thinking netfabb?
22:35 < delinquentme_> waz told today that this is a pretty solid cad design package too: http://www.plm.automation.siemens.com/en_us/products/nx/for-design/
22:35 < kanzure> tinkercad upverter emachineshop shapesmith verbnurbs parametricparts floodeditor.com the list is really long don't make me think this hard
22:36 < nmz787> no I don't think it was netfabb, I don't remember seeing that
22:36 < nmz787> maybe floodeditor
22:36 < kanzure> fenn: what's worse is that i have no way of knowing if it was scrobbled or not http://diyhpl.us/~bryan/irc/trance
22:36 < nmz787> which is down for me
22:38 < nmz787> 'like most cloud-based applications, your data is lost to you as long as the server is down'
22:39 < kanzure> is that from the song?
22:40 < fenn> .ety scrobble
22:40 < yoleaux> Sorry, I couldn't find the etymology of that.
22:40 < kanzure> the thing that dumps names of things into another thing while you are listening to some of those things
22:40 < fenn> post-post-postmodern hangover trance
22:41 < fenn> is "scrobble" a fancy word for "log"
22:42 < kanzure> yes
22:42 < fenn> i would like to do logging with mocp
22:43 < nmz787> hmm, I got something to work in verb with a nurbsSurface finally
22:43 < fenn> i dont get the need for a connection to last.fm
22:44 < nmz787> so how is it that verb is able to 'render' nice looking things faster than implicitCAD?
22:44 < kanzure> that's so they can monetize your data
22:44 < nmz787> or smoother rather
22:44 < nmz787> faster and smoother
22:44 < kanzure> webgl magic
22:46 < fenn> i dont want anyone monetizing my data
22:46 < fenn> i just want the data
22:46 < fenn> it seems lastfmsubmitd saves logs and uses logrotate at least
23:00 -!- delinquentme_ [~dingo@74.61.157.78] has quit [Ping timeout: 250 seconds]
23:24 < nmz787> damn, this looks really really cool http://portland.craigslist.org/clk/pho/4819884558.html
23:24 < nmz787> .title
23:24 < yoleaux> Zeiss Interferometer / microscope
23:25 < nmz787> $1000 though
23:25 < nmz787> if the action is smooth though, that would be chump change for a stepper platform
23:29 < nmz787> unless it actually does .wik Classical interference microscopy
23:29 < nmz787> .wik Classical interference microscopy
23:29 < yoleaux> "Classical interference microscopy (also referred to as quantitative interference microscopy) uses two separate light beams with much greater lateral separation than that used in phase contrast microscopy or in differential interference microscopy (DIC)." — http://en.wikipedia.org/wiki/Classical_interference_microscopy
23:58 -!- rscnt [~rscnt@190.62.215.21] has joined ##hplusroadmap
--- Log closed Thu Jan 01 00:00:09 2015