--- Log opened Thu Jul 23 00:00:19 2015 00:29 -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 244 seconds] 00:32 -!- nottimschmidt [~timschmid@35.10.220.134] has joined ##hplusroadmap 00:38 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-pcqtpvcuhiitjabd] has joined ##hplusroadmap 00:56 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-pcqtpvcuhiitjabd] has quit [Ping timeout: 240 seconds] 00:56 -!- Madplatypus is now known as Pathos02 00:59 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-vtnfasbioortpmnb] has joined ##hplusroadmap 00:59 -!- Pathos02 is now known as Madplatypus 01:02 -!- sheena [~home@d99-199-117-38.bchsia.telus.net] has quit [Ping timeout: 256 seconds] 01:43 -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap 02:00 -!- zadock [~outsider@cthulhu.tuiasi.ro] has joined ##hplusroadmap 02:26 -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 244 seconds] 02:28 -!- nottimschmidt [~timschmid@35.10.220.134] has joined ##hplusroadmap 02:30 < JayDugger> drethelin: "affordable" "rapid" for aerospace and governmental values of the adjectives 02:43 < JayDugger> Notice all the authors work for NASA? 02:57 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Read error: Connection reset by peer] 03:08 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 03:36 < archels> can anyone grab this paper? http://www.mitpressjournals.org/doi/abs/10.1162/NECO_a_00753 03:38 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-vtnfasbioortpmnb] has quit [Quit: Connection closed for inactivity] 03:45 < Betawolf> http://moscow.sci-hub.bz/f64e3b7d1361d9cec9a4fa4680331200/krunglevicius2015.pdf 03:46 < Betawolf> archels: ^ 03:49 < archels> cheers! 03:58 -!- Acty [uid89656@gateway/web/irccloud.com/x-tgcfzvhkysjrwgid] has joined ##hplusroadmap 04:06 -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-orxtnusqlnngegxn] has quit [Quit: Connection closed for inactivity] 04:11 -!- zadock [~outsider@cthulhu.tuiasi.ro] has quit [Quit: Leaving] 04:50 < archels> When analyzing data, scientists generally run a test for something called “statistical significance.” 05:26 -!- eudoxia [~eudoxia@r167-57-78-176.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap 05:53 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 06:14 -!- sheena [~home@d99-199-117-38.bchsia.telus.net] has joined ##hplusroadmap 06:36 -!- Quashie__ [~boingredd@50.14.92.17] has joined ##hplusroadmap 06:39 -!- Quashie_ [~boingredd@50.14.92.17] has quit [Ping timeout: 240 seconds] 06:46 -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 272 seconds] 06:47 -!- nottimschmidt [~timschmid@35.10.220.134] has joined ##hplusroadmap 06:58 -!- Burnin8 [~Burn@pool-71-241-254-153.washdc.fios.verizon.net] has quit [Ping timeout: 244 seconds] 07:05 -!- sheena [~home@d99-199-117-38.bchsia.telus.net] has quit [Ping timeout: 246 seconds] 07:12 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hhuhpawhuynuymlh] has joined ##hplusroadmap 07:25 -!- Urchin [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 08:42 -!- helleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has quit [Read error: Connection reset by peer] 08:52 < kanzure> .title http://www.mitpressjournals.org/doi/abs/10.1162/NECO_a_00753 08:52 < yoleaux> An Error Occurred Setting Your User Cookie 08:52 < kanzure> bah 08:52 < kanzure> worthless 08:59 < kanzure> http://techcrunch.com/2015/07/23/ginkgo-bioworks-takes-on-zymergen-with-45-million-in-series-b-funding/ 09:01 < kanzure> welp our mole probably got $50k of that and it probably vests over 1 bajillion years 09:01 < kanzure> "Rose fragrance is a scarce commodity, with a major looming global shortage. However, Ginkgo was able to recreate a synthetic and genetically identical rose oil in mass quantities using Bioworks1" 09:02 < kanzure> there's a shortage of smelling like shit? 09:03 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 255 seconds] 09:11 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 09:13 -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-wkrzraixviuvysnz] has joined ##hplusroadmap 09:28 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 264 seconds] 09:29 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Remote host closed the connection] 09:30 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap 09:32 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Remote host closed the connection] 09:32 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap 09:33 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Remote host closed the connection] 09:33 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap 09:33 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Remote host closed the connection] 09:34 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap 09:46 -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-wkrzraixviuvysnz] has quit [] 09:46 -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-sudnxpbgqavprhef] has joined ##hplusroadmap 09:56 < kanzure> bloop 10:05 < kanzure> .title http://worldbuilding.stackexchange.com/questions/14442/how-to-eat-the-moon 10:05 < yoleaux> space - How To Eat the Moon - Worldbuilding Stack Exchange 10:05 < kanzure> .title http://www.phys.unsw.edu.au/~jw/cryoblurb.html 10:05 < yoleaux> Cryobiology and anhydrobiology - physical stresses in cells at low temperature and/or low hydration 10:06 < kanzure> https://www.reddit.com/r/space/comments/2f8ln3/dyson_spheres_seem_easier_than_people_think/ 10:07 < kanzure> ugh? https://www.reddit.com/r/rational 10:08 < eudoxia> good christ 10:10 < kanzure> eudoxia: report? 10:10 < eudoxia> what 10:11 < kanzure> it's a more formal way of saying "sup" 10:12 < kanzure> have you been replaced by a can of red bull yet? 10:12 < eudoxia> oh, right. nothing. i got exams coming up. 10:12 < eudoxia> you know i've never actually tried red bull 10:14 < eudoxia> what's up on your end kanzure 10:14 < kanzure> still bitcoin stuff 10:15 < eudoxia> what sort of bitcoin stuff 10:16 < kanzure> see pm 10:21 -!- balrog [~balrog@unaffiliated/balrog] has quit [Remote host closed the connection] 10:23 -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 244 seconds] 10:26 -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap 10:28 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hhuhpawhuynuymlh] has quit [Quit: Connection closed for inactivity] 10:45 -!- helleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap 10:45 < CaptHindsight> kanzure: the wash step is after the coupling so the oligo strands don't wash away, sorry it's taken 12 hrs+ to get back to you 10:47 < CaptHindsight> step 1) bond the first base to a silane coated slide or a glass bead 10:50 < CaptHindsight> that base is going to be stuck there until you cleave it 10:51 < CaptHindsight> you can add as many bases as you wish to it in a linear fashion 10:51 < CaptHindsight> lets just say 20 for the example 10:52 < CaptHindsight> you leave the oligo bonded to the slide or tray that you want to build on... 10:54 < CaptHindsight> lets call this oligo the "foundation", I haven't found a coined term yet for this yet 10:54 -!- delinquentme [~delinquen@68.65.85.43] has joined ##hplusroadmap 10:55 < CaptHindsight> the oligo that you wish to be bonded to the foundation is cleaved from the tray or bead and coupled together 10:55 < kanzure> the was hstep is after the coupling, yes, but if you want to get two oligos together and then have a wash step, you're going to wash away those oligos :-( 10:55 < CaptHindsight> now you have a oligo 20 bases long 10:56 < CaptHindsight> kanzure: nope, since the first oligo is still bonded to the tray or bead 10:56 < kanzure> yes, but if you want to assemble two oligos together, they have to be free-floating 10:57 < CaptHindsight> only one end has to be from the foundation 10:58 < CaptHindsight> one is anchored at one end with the other end free for coupling, the second oligo may be free floating 10:59 < kanzure> it's not clear whether the dna ligase enzyme is compatible with that scheme 10:59 < CaptHindsight> there are 10:59 < CaptHindsight> I've been looking into the different agents for capping, coupling, cleaving etc 11:01 < CaptHindsight> it looks to me that most authors don't really know how to put the steps together in writing or they are purposely obfuscating this 11:01 -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 244 seconds] 11:02 < CaptHindsight> they read like most textbooks, explanations designed to be memorized vs understood 11:02 < kanzure> ok so you want to do on-bead gene assembly 11:02 < kanzure> or solid-support gene assembly 11:02 -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap 11:04 < kanzure> http://labs.domipheus.com/blog/designing-a-cpu-in-vhdl-part-1-rationale-tools-method/ 11:04 < kanzure> http://labs.domipheus.com/blog/designing-a-cpu-in-vhdl-part-2-xilinx-ise-suite-register-file-testing/ 11:04 < kanzure> http://labs.domipheus.com/blog/designing-a-cpu-in-vhdl-part-3-instruction-set-decoder-ram/ 11:04 < kanzure> http://labs.domipheus.com/blog/designing-a-cpu-in-vhdl-part-4-alu-comparisons-branching/ 11:04 < kanzure> http://labs.domipheus.com/blog/designing-a-cpu-in-vhdl-part-5-pipeline-and-control-unit/ 11:04 < kanzure> http://labs.domipheus.com/blog/designing-a-cpu-in-vhdl-part-6-program-counter-instruction-fetch-branching/ 11:05 < CaptHindsight> with inkjet printing on a slide you can make thousands of short oligos 11:06 < CaptHindsight> that slide can anchor the "foundations" 11:08 < CaptHindsight> the next oligos can be cleaved off the slide and moved to the foundations using light or electrostatic charge 11:08 < CaptHindsight> so they are still all on the same slide or tray 11:08 < CaptHindsight> this may be done in parallel 11:09 < CaptHindsight> so you can have 10, 100 etc foundations that remain bonded to the slide 11:10 < CaptHindsight> and then cleave the next oligos and direct them to the foundations for coupling 11:13 < CaptHindsight> cleaving may be selective with a laser 11:15 < kanzure> not sure how to do it with light- we haven't figured that out yet 11:15 < CaptHindsight> I saw some photocleavable couplings that when activated leave the ends free to be bonded 11:15 < kanzure> all of the light-based actuation methods work for only very small surface areas, and don't work for very tiny droplets. 11:15 < kanzure> or rather, they work for tiny droplets if you have lots of optics 11:15 < kanzure> or if you fabricate a microelectrode array 11:16 < CaptHindsight> either and the array would be the same slide that you inkjet the drop onto 11:16 < kanzure> if you have a large microelectrode array that can move tiny droplets, then you don't need an inkjet printhead i think 11:17 < kanzure> well, nevermind, i take that back, it's more convenient to use an inkjet head 11:17 < CaptHindsight> true, but the inkjet builds lots of drops faster 11:17 < kanzure> ok. so now we need to fabricate a microelectrode array that can move 100k spots simultaneously or whatever. 11:17 < CaptHindsight> not all at the same time 11:18 < kanzure> and which method are you talking about for re-linking an oligo? 11:18 < CaptHindsight> say you have a 100 x 100 array 11:18 < kanzure> if you don't do it all at the same time, then you might as well just use slow pipettes 11:18 < CaptHindsight> the first row of 100 could be the foundations 11:19 < CaptHindsight> then row 2 is cleaved drop by drop and moved to combine with the foundation in its same column 11:21 < CaptHindsight> to link a foundation to the next free floating oligo we have to decide which method 11:21 < CaptHindsight> there are a few to choose from 11:23 < CaptHindsight> http://www.genelink.com/newsite/products/mod_detail.asp?modid=133 photocleavable linker 11:29 < CaptHindsight> kanzure: 100um spots are easy to achieve with a laser for triggering a cleave 11:37 < CaptHindsight> http://imagebin.ca/v/29eLd1TYvEx9 summary of DNA microarrays 11:40 < CaptHindsight> inkjet has the cheap chemistry to build the oligos what did we say 50-100 bases long 11:41 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 11:41 < CaptHindsight> we can also fabricate an electrode array cheaply that is also the slide/tray 11:43 < CaptHindsight> the change in the first step to the inkjet might be printing a photocleavable bond 11:44 < CaptHindsight> then the rest of the 50-100 bases are done as they did with POSaM 11:45 < CaptHindsight> and since the photocleaveble bonding agent is a bit more expensive we only use it once per 50 to 100 bases, so we use 1/50 to 1/100 as much as if it is used for every base 11:48 < CaptHindsight> they are sensitive to 300nm - 350nm 11:49 -!- delinquentme [~delinquen@68.65.85.43] has quit [Ping timeout: 246 seconds] 11:49 < CaptHindsight> they don't have a graph so I don't know where exactly the sensitivity drops off but... 11:50 < CaptHindsight> it;s mist likely well under 400nm so we could use a UV laser to cleave and use visible spectrum for light guided/activated 11:50 < CaptHindsight> mist/most 11:52 < kanzure> hmm. 11:52 < kanzure> so we would be using a single laser to move >100k droplets? 11:52 < CaptHindsight> laser to cleave 11:52 < CaptHindsight> light or electrostatic to move drops 11:52 < CaptHindsight> visible light 11:53 < CaptHindsight> UV laser to cleave 11:53 < kanzure> "the photocleaveble bonding agent is a bit more expensive we only use it once per 50 to 100 bases" 11:53 < kanzure> what does this mean? reconnecting an oligo to the surface? 11:53 < CaptHindsight> I can clarify 11:55 < CaptHindsight> they are only anchored once to the tray/slide using the photocleaveble 11:55 < kanzure> and your plan is to see if dna ligase can combine a free-floater oligo with a surface-immobilized oligo? 11:55 < CaptHindsight> for the first base or each drop by inkjet to the slide/tray/electrostatic array 11:56 < kanzure> i think there should be a writeup 11:56 < CaptHindsight> thats how it's done 11:56 < kanzure> (and also, i'm distracted writing bitcoin software at the moment...) 11:56 < CaptHindsight> yeah, no problem 11:57 < CaptHindsight> many papers and website that offer this info also mention how these steps or products are used in patented DNA synthesizers 11:58 < kanzure> there's no dna synthesizer on the market that uses dna ligase 11:58 < kanzure> (or the previous markets for that matter) 11:58 < CaptHindsight> I think this is semantics at work here, let me clarify 11:59 < CaptHindsight> you can use the same chemistry to bond an anchored oligo to a free floating oligo as it used to build each oligo 11:59 < CaptHindsight> it/is 12:00 -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 260 seconds] 12:03 < kanzure> is that true? i think you have only one 5' end. 12:11 < CaptHindsight> you can choose what stays capped and what gets deprotected 12:12 < CaptHindsight> all the chems are there I bet it's patents that keep all the lowest cost tech from ending up in the same machine 12:13 -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap 12:17 < kanzure> yes but what's the mechanism? which chemistry and which reactions? 12:17 < kanzure> these are things that we need to know in order to implement this 12:20 < CaptHindsight> http://www.genelink.com/newsite/products/modoligosENDMODIFIERS.asp for example 12:22 < CaptHindsight> inkjet to build the oligos from 1 - 100 bases long 12:23 < kanzure> i don't think any of those linkers allow other oligos to be attached 12:23 < kanzure> not sure 12:23 < CaptHindsight> electrostatic to move the oligos for bonding to each other 12:24 < kanzure> "The end modifiers add a reactive functional group which can be used for conjugation" conjugation with what 12:26 < CaptHindsight> where did the biochemists go in here? 12:26 < CaptHindsight> looks like I'm the only one plowing through this 12:27 -!- eudoxia [~eudoxia@r167-57-78-176.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] 12:28 < CaptHindsight> kanzure: there are several chem suppliers 12:29 < CaptHindsight> I'd suggest creating a side by side comparison of costs and flow chart for each step of each process available 12:29 < CaptHindsight> then you can easily see which ones are more desired based on price and tech available for moving chems and oligos around 12:31 < kanzure> i just need the chemistry right now. no handwaving- just something that will work. 12:32 < CaptHindsight> looks like a combo of photoreactive and Phosphoramidite 12:33 < CaptHindsight> http://www.jnanobiotechnology.com/content/9/1/57/figure/F1 but rather than use light deprotection in every cycle... 12:34 < CaptHindsight> light deprotection is only used for every 50 or 100 cycles 12:35 < CaptHindsight> they dry with helium btw 12:35 < CaptHindsight> wow, no expense spared 13:34 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Quit: punk] 13:38 -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap 13:40 < CaptHindsight> kanzure: is there a wiki for this channel? 13:41 < chris_99> http://diyhpl.us/wiki 13:50 < kanzure> CaptHindsight: http://diyhpl.us/wiki/dna/synthesis/notes/ 13:56 -!- FourFire [~FourFire@246-246-11.connect.netcom.no] has joined ##hplusroadmap 14:02 -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 255 seconds] 14:35 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Ping timeout: 250 seconds] 14:37 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 14:48 -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 256 seconds] 14:53 -!- sandeep_ [~sandeep@111.235.64.135] has joined ##hplusroadmap 15:02 -!- drewbot [~cinch@ec2-54-242-1-227.compute-1.amazonaws.com] has quit [Remote host closed the connection] 15:02 -!- drewbot [~cinch@ec2-54-159-17-190.compute-1.amazonaws.com] has joined ##hplusroadmap 15:14 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 255 seconds] 15:21 -!- FourFire [~FourFire@246-246-11.connect.netcom.no] has quit [Ping timeout: 244 seconds] 15:26 -!- FourFire [~FourFire@201-251-11.connect.netcom.no] has joined ##hplusroadmap 15:35 -!- yashgaroth [~ffffff@2602:306:35fa:d500:3cf0:b827:7f04:faf3] has joined ##hplusroadmap 15:40 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 15:45 -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap 15:51 -!- delinquentme [~delinquen@74.61.157.78] has quit [Remote host closed the connection] 15:52 -!- drethelin [drethelin@71-87-115-157.dhcp.mdsn.wi.charter.com] has quit [] 15:53 -!- FourFire [~FourFire@201-251-11.connect.netcom.no] has quit [Ping timeout: 240 seconds] 16:01 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Ex-Chat] 16:01 -!- balrog [~balrog@unaffiliated/balrog] has joined ##hplusroadmap 16:55 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Quit: austerity chic brand destruction] 16:57 < kanzure> .title https://news.ycombinator.com/item?id=9937372 16:57 < yoleaux> Optimizing CRISPR: Sub-second searches on a 3 billion base genome | Hacker News 16:58 -!- drethelin [drethelin@71-87-115-157.dhcp.mdsn.wi.charter.com] has joined ##hplusroadmap 17:02 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 17:02 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has left ##hplusroadmap ["not here"] 17:08 -!- sandeep_ [~sandeep@111.235.64.135] has quit [Quit: Leaving] 18:15 -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 244 seconds] 18:15 -!- cluckj [~cluckj@pool-108-16-231-242.phlapa.fios.verizon.net] has joined ##hplusroadmap 18:21 < kanzure> cluckj: welcome back 18:21 < cluckj> thanks 18:26 < cluckj> what's up? 18:28 < mgin> hey 19:03 -!- Porb [~Porbus@c110-22-139-137.mckinn4.vic.optusnet.com.au] has joined ##hplusroadmap 19:31 < kanzure> http://i.imgur.com/k8FKq6H.jpg 19:33 < mgin> disgusting 19:33 < mgin> i really don't like you 19:34 < yashgaroth> ok mgin you have finally proven yourself to be enough of a dick, I shall now reveal to you the secrets of eternal life 19:34 < mgin> ban? 19:35 < yashgaroth> if you like 19:35 < mgin> no 19:38 < kanzure> mgin: please continue 19:40 < mgin> that was an offensive image 19:40 < mgin> and you refuse to answer any questions in a straight manner 19:40 < mgin> that's all. 19:40 < kanzure> can you give me an example of what you would consider a straight answer? 19:41 < mgin> yes, this response to your question that I am making right now. 19:41 < mgin> would be an example of a straight answer 19:41 < kanzure> and "look at our plans" is not a straight answer to your question "what are you doing"? 19:41 < mgin> see how i responded directly to the question asked of me, in english? 19:41 < mgin> i didn't link you to anything or ignore you at all 19:41 < mgin> i just wrote out a coherent, direct response 19:42 < kanzure> perhaps you hate hyperlinks? 19:42 < mgin> that's all. 19:43 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 19:56 < ParahSailin_> kanzure: yeah its like they hadnt heard of reference alignment and made their own crappy crap from scratch 19:56 < ParahSailin_> bwa would have been orders of magnitude faster if they had bothered to do a simple literature search on a not-new-at-all subject 19:57 < ParahSailin_> points for trying i guess 20:00 < cluckj> what the fuck did I come back to? 20:01 -!- Porb__ [~Porbus@c110-22-139-137.mckinn4.vic.optusnet.com.au] has joined ##hplusroadmap 20:02 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Read error: Connection reset by peer] 20:03 -!- Porb [~Porbus@c110-22-139-137.mckinn4.vic.optusnet.com.au] has quit [Ping timeout: 265 seconds] 20:08 < kanzure> cluckj: things that are up, http://diyhpl.us/wiki/dna/synthesis/notes/ 20:13 < cluckj> sweet 20:29 < cluckj> I am still writing...should be done with a first draft soon 20:33 < cluckj> for varying values of "soon" 20:35 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-atqsfdkmwtrckcyg] has joined ##hplusroadmap 21:22 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 21:28 -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap 21:52 -!- drethelin [drethelin@71-87-115-157.dhcp.mdsn.wi.charter.com] has quit [Ping timeout: 252 seconds] 22:10 -!- yashgaroth [~ffffff@2602:306:35fa:d500:3cf0:b827:7f04:faf3] has quit [Quit: Leaving] 22:12 -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 246 seconds] 22:28 -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap 22:33 -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 252 seconds] 22:49 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Read error: Connection reset by peer] 23:07 -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap 23:29 -!- Quashie__ [~boingredd@50.14.92.17] has quit [Ping timeout: 240 seconds] 23:44 < justanotheruser> erasmus: is there something you wanted? 23:48 -!- juul is now known as juulie 23:51 -!- juulie is now known as MrJuulie 23:57 -!- erasmus [~esb@unaffiliated/erasmus] has quit [Quit: Namaste ] 23:59 -!- FourFire [~FourFire@50-164-11.connect.netcom.no] has joined ##hplusroadmap --- Log closed Fri Jul 24 00:00:20 2015