--- Log opened Sun May 29 00:00:14 2016 00:00 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-vhysfgkwaausoqwf] has quit [Quit: Connection closed for inactivity] 00:34 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Ping timeout: 264 seconds] 01:11 < jrayhawk> http://time.com/money/4350788/west-virginia-town-for-sale-1-million/ who wants a ready-made cult compound? 02:48 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 260 seconds] 03:05 < kanzure> maybe me, thanks 03:06 < kanzure> no no, wait, i said i would work on this 03:35 -!- augur [~augur@76-218-206-38.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 03:40 -!- augur [~augur@76-218-206-38.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 260 seconds] 03:43 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-yodjbzroansmoxeb] has joined ##hplusroadmap 03:56 -!- Gurkenglas [~Gurkengla@dslb-178-000-182-226.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 04:03 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 04:11 < FourFire> kanzure, the supposed nootropic "Synapsyl" is all bullshit right? 04:15 < FourFire> Yeah, it's a scam. 04:33 -!- ArturSha1 [~ArturShai@185.66.252.126] has joined ##hplusroadmap 04:43 -!- Orpheon [~Orpheon@213.200.193.129] has joined ##hplusroadmap 05:10 -!- hehelleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap 05:14 -!- helleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 246 seconds] 05:23 -!- Guest60221 [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap 05:26 -!- hehelleshin [~talinck@66-161-138-110.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 246 seconds] 05:36 -!- augur [~augur@76-218-206-38.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 05:41 -!- augur [~augur@76-218-206-38.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 276 seconds] 05:47 -!- Gurkenglas [~Gurkengla@dslb-178-000-182-226.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 252 seconds] 05:50 -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-yodjbzroansmoxeb] has quit [Quit: Connection closed for inactivity] 06:03 -!- Gurkenglas [Gurkenglas@dslb-178-000-182-226.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 06:12 -!- Gurkenglas [Gurkenglas@dslb-178-000-182-226.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 06:21 < maaku_> kanzure: no wifi :( 06:32 -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap 06:38 < justanotheruser> oh no kanzure! 06:38 < justanotheruser> github is removing streaks 06:44 -!- Orpheon [~Orpheon@213.200.193.129] has quit [Remote host closed the connection] 06:47 -!- Orpheon [~Orpheon@213.200.193.129] has joined ##hplusroadmap 06:51 -!- fleshtheworld [~fleshthew@2602:306:cf0f:4c20:b01c:2800:62b7:a30e] has quit [Read error: Connection reset by peer] 07:15 -!- Quashie_ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has joined ##hplusroadmap 07:18 -!- Quashie [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has quit [Ping timeout: 240 seconds] 07:29 -!- augur [~augur@2602:304:cdac:e260:3818:7e0a:b881:2e27] has joined ##hplusroadmap 07:34 < kanzure> "Google paid $500,000,000 to DoJ to avoid felony charges after they were caught knowingly selling AdWords to an FBI undercover operation pretending to be a Mexican drug lord trying to take over the US steroids market" http://searchengineland.com/wsj-government-sting-google-pharma-ads-109247 07:44 < maaku_> can i get a tax refund from that? 07:48 < kanzure> yes but only upon presentation of a valid steroid receipt 08:40 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Read error: Connection reset by peer] 08:42 -!- nildicit [~nildicit@unaffiliated/nildicit] has quit [Ping timeout: 240 seconds] 08:42 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 08:46 -!- jtimon [~quassel@4.28.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 08:59 -!- jaboja [~jaboja@5.196.225.175] has joined ##hplusroadmap 09:33 -!- jaboja [~jaboja@5.196.225.175] has quit [Ping timeout: 240 seconds] 09:50 -!- Gurkenglas [Gurkenglas@dslb-178-000-182-226.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 09:51 < maaku_> http://www.openphilanthropy.org/focus/global-catastrophic-risks/potential-risks-advanced-artificial-intelligence/what-should-we-learn-past-ai-forecasts 09:51 < maaku_> .title 09:51 < yoleaux> What should we learn from past AI forecasts? | Open Philanthropy Project 09:55 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 09:56 -!- Aurelius_Laptop [~cpopell@static-108-28-104-83.washdc.fios.verizon.net] has joined ##hplusroadmap 10:00 -!- CuriousCat [~CuriousCa@unaffiliated/wye-naught/x-8734122] has joined ##hplusroadmap 10:36 -!- Aurelius_Laptop [~cpopell@static-108-28-104-83.washdc.fios.verizon.net] has quit [Ping timeout: 260 seconds] 11:18 < kanzure> http://goodcomics.comicbookresources.com/wp-content/uploads/2009/02/flashbomb1.jpg 11:32 -!- sandeepkr [~sandeep@111.235.64.4] has quit [Ping timeout: 260 seconds] 11:36 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 246 seconds] 11:37 < maaku_> Their flesh torn apart by nuclear reactions as particles of air impact their skin at relativistic velocities 11:37 < maaku_> https://what-if.xkcd.com/1/ 11:40 -!- AmbulatoryCortex [~Ambulator@173-31-155-69.client.mchsi.com] has joined ##hplusroadmap 11:46 -!- sandeepkr [~sandeep@111.235.64.4] has joined ##hplusroadmap 11:47 -!- nildicit [~nildicit@unaffiliated/nildicit] has joined ##hplusroadmap 11:59 < kanzure> maaku_: no you see, there was also a molecular demon sorting out any air particles not at the right velocities 12:11 -!- strangewarp_ [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 12:13 -!- ArturSha1 [~ArturShai@185.66.252.126] has quit [Ping timeout: 264 seconds] 12:14 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Ping timeout: 264 seconds] 12:36 < jcorgan> in some tiny fraction of the multiverse they just quantum tunneled away 12:37 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-bvhtzzrazndchnww] has joined ##hplusroadmap 12:51 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 12:53 < kanzure> oh i mean maxwell's demon i guessw 13:05 -!- c0rw1n [~c0rw1n@124.213-241-81.adsl-dyn.isp.belgacom.be] has quit [] 13:09 -!- c0rw1n [~c0rw1n@124.213-241-81.adsl-dyn.isp.belgacom.be] has joined ##hplusroadmap 13:14 < maaku_> Right makes sense. Carry on. 14:10 -!- CuriousCat [~CuriousCa@unaffiliated/wye-naught/x-8734122] has quit [Ping timeout: 250 seconds] 14:13 -!- Quashie__ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has joined ##hplusroadmap 14:16 -!- Quashie_ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has quit [Ping timeout: 272 seconds] 14:25 -!- sandeepkr [~sandeep@111.235.64.4] has quit [Ping timeout: 272 seconds] 14:26 -!- Quashie_ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has joined ##hplusroadmap 14:28 -!- Quashie [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has joined ##hplusroadmap 14:29 -!- Quashie__ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has quit [Ping timeout: 244 seconds] 14:31 -!- Quashie_ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has quit [Ping timeout: 272 seconds] 14:42 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-bvhtzzrazndchnww] has quit [Quit: Connection closed for inactivity] 15:02 -!- Quashie [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has quit [Read error: Connection reset by peer] 15:02 -!- panax [~panax@71.101.252.187] has quit [Read error: Connection reset by peer] 15:02 -!- Quashie_ [~boingredd@CPEbc4dfb5bf733-CMbc4dfb5bf730.cpe.net.cable.rogers.com] has joined ##hplusroadmap 15:07 -!- panax [~panax@71.101.252.187] has joined ##hplusroadmap 15:24 -!- CuriousCat [~CuriousCa@unaffiliated/wye-naught/x-8734122] has joined ##hplusroadmap 15:25 -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap 15:45 < kanzure> received this from some bcc-only email a moment ago, 15:45 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/A%20hypothetical%20protocol%20for%20DIYBIO%20DNA%20synthesis.pdf 15:45 < kanzure> yashgaroth: ^ 16:00 < yashgaroth> ok 1) the spelling and grammar omg 2) type IIS restriction enzymes require a double-stranded target at the cleavage site, and while he types a page-long paragraph about getting around that, it's still a clusterfuck to generate ssDNA oligos 3) idk why he suggests PAGE for purification and not HPLC but that's not a major issue [...] 16:01 < yashgaroth> I mean if the TDT stuff works for generating template then great let's go with that, but I figured the much easier way was to generate template with that and then use a nicking enzyme + polymerase like I've said before 16:06 -!- Aurelius_Laptop [~cpopell@c-73-200-185-48.hsd1.va.comcast.net] has joined ##hplusroadmap 16:10 < kanzure> yashgaroth: i would like some assistance on finding a chemist person to help with the project. i can pay. 16:11 < yashgaroth> hey if the TDT thing works you don't even need a chemist 16:16 < kanzure> er.. 16:18 < yashgaroth> well it's entirely enzymatic aside from the thermolabile nucleotides, which can't be that hard, and I guess the template conjugation to the surface, but just buy biotinylated oligos like w/e 16:20 < kanzure> well the chemist is not just for the chemistry it's for the endless tweaking that literally all biology projects require 16:23 < yashgaroth> the guy who wrote this is a chemist, no? I assume he's not a biologist, since the stuff he writes about hairpins and methylated DNA would be very disappointing 16:24 < kanzure> mathematician 16:24 < kanzure> topoogically complex curve ball, isn't this 16:24 < yashgaroth> that explains a lot 16:26 < yashgaroth> just saying, you may need a tweaker but not necessarily a chemist, optimizing and troubleshooting is most of most bio jobs 16:27 < kanzure> yeah good point, i don't see a strong reason to prefer a *chemist* 16:29 < kanzure> wait why isn't it you 16:30 < yashgaroth> I should know in a couple weeks whether I'm available, since that thing in Seattle may have been funded, in which case I'll need to drop everything 16:30 < nmz787> kanzure: well they obviously need to do this in microfluidics for the lack of yield on non-porous glass issue 16:31 < nmz787> I heard the whatever-labile nucleotides were expensive, last time i checked years ago 16:37 < nmz787> http://www.trilinkbiotech.com/cart/scripts/prodView.asp?idproduct=4665 16:37 < nmz787> .title 16:37 < yoleaux> CleanAmp™ dNTP Set 16:37 < nmz787> 2 µmoles each (4 x 2) ($84.00) --- 10 µmoles each (4 x 10) ($310) 16:38 < yashgaroth> if you're only generating a template array with them and then amplifying off that (with nicking enzyme + polymerase *cough*), then they won't be getting consumed after the initial setup 16:38 < nmz787> de novo 4 life yo 16:39 * nmz787 back to implementing a pipeline for BRLCAD to g-code 17:29 -!- Darius [~quassel@cpe-158-222-160-123.nyc.res.rr.com] has joined ##hplusroadmap 17:31 -!- JayDugger [~jwdugger@108.19.186.58] has quit [Read error: Connection reset by peer] 17:41 < kanzure> nmz787: linuxcnc probably has something for that already 17:46 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Remote host closed the connection] 17:47 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 18:04 < jrayhawk> http://psybersecurity.tumblr.com/post/144607589631/five-years-later 18:33 -!- Aurelius_Laptop [~cpopell@c-73-200-185-48.hsd1.va.comcast.net] has quit [Ping timeout: 260 seconds] 18:39 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 276 seconds] 18:47 -!- Gurkenglas [Gurkenglas@dslb-178-000-182-226.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 19:01 -!- strangewarp_ [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Read error: Connection reset by peer] 19:01 -!- strangewarp_ [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 19:25 -!- drewbot_ [~cinch@ec2-54-198-57-201.compute-1.amazonaws.com] has quit [Remote host closed the connection] 19:25 -!- drewbot [~cinch@ec2-54-167-187-37.compute-1.amazonaws.com] has joined ##hplusroadmap 19:34 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 19:40 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 264 seconds] 19:45 -!- AmbulatoryCortex [~Ambulator@173-31-155-69.client.mchsi.com] has quit [Quit: Leaving] 19:50 < maaku_> c0rw1n: or "derp learning" 19:52 < c0rw1n> ? 19:53 < c0rw1n> ( also, lol ) 20:40 -!- anoncicada [anoncicada@gateway/shell/elitebnc/x-tfehosltzskbfkba] has quit [Quit: faggory daggory doo!] 20:45 -!- sandeepkr [~sandeep@111.235.64.4] has joined ##hplusroadmap 20:46 -!- ArturSha1 [~ArturShai@185.66.252.126] has joined ##hplusroadmap 20:46 -!- anoncicada [anoncicada@gateway/shell/elitebnc/x-dytaijkfstfneawh] has joined ##hplusroadmap 20:47 -!- sandeepkr [~sandeep@111.235.64.4] has quit [Max SendQ exceeded] 20:48 -!- sandeepkr [~sandeep@111.235.64.4] has joined ##hplusroadmap 20:51 -!- anoncicada [anoncicada@gateway/shell/elitebnc/x-dytaijkfstfneawh] has quit [Changing host] 20:51 -!- anoncicada [anoncicada@unaffiliated/anarcanon] has joined ##hplusroadmap 20:51 -!- anoncicada [anoncicada@unaffiliated/anarcanon] has quit [Changing host] 20:51 -!- anoncicada [anoncicada@gateway/shell/elitebnc/x-dytaijkfstfneawh] has joined ##hplusroadmap 21:09 -!- Orpheon [~Orpheon@213.200.193.129] has quit [Read error: Connection reset by peer] 21:12 -!- Darius [~quassel@cpe-158-222-160-123.nyc.res.rr.com] has quit [Remote host closed the connection] 21:19 -!- esmerelda [~andares@unaffiliated/jacco] has joined ##hplusroadmap 21:32 < xentrac_> nmz787: you may enjoy http://hackaday.com/2016/04/11/continuing-the-dialog-its-time-the-software-people-and-mechanical-people-sat-down-and-had-a-talk/ 22:07 < nmz787> xentrac_: just gave it a quick once through 22:07 < nmz787> may touch upon the links later 22:19 -!- esmerelda [~andares@unaffiliated/jacco] has quit [Ping timeout: 276 seconds] 22:35 < abetusk> nmz787, any links to the brlcad api/library you re using to export? 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