--- Log opened Mon Jul 25 00:00:06 2016
00:01 < maaku> kanzure: had to. there's no way pokemon go doubled nintendo's real value
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06:20 < JayDugger> I don't know, maaku. Pokémon Go demonstrated a new model of gaming possible for Nintendo that doesn't involve manufacturing or consoles.
06:21 < JayDugger> They have still more markets in which to release the game, and they have other IP they can release as AR games.
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06:55 < archels> https://www.jwz.org/blog/2015/06/from-the-gnome-user-interface-guidelines/
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08:07 < kanzure> fenn: nectome is in oakland
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10:34 < CaptHindsight> "can you come to our location and setup a demo sequencer?"
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10:34 < CaptHindsight> sure if you pay full price and wait 3-6 months for delivery
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10:39 < nmz787_i> sup
10:40 < kanzure> nmz787_i: lotta additions to the doc, plz read.
10:41 < nmz787_i> motorcycling on gravel yesterday was exhilarating... adrenaline was pretty close if not on full-blast
10:41 < nmz787_i> if I open the doc on my work laptop, I think that means the company owns it
10:41 < nmz787_i> maybe not
10:42 < chris_99> i've heard something like that, if you work on something in company time
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10:43 < nmz787_i> its def part of TV show plots
10:43 < chris_99> yeah i think i heard it on Silicon Valley heh
10:44 < nmz787_i> yeah
10:44 < nmz787_i> I wonder if there is a way to make that work to our advantage
10:44 < nmz787_i> like use some kind on infectious license or something
10:45 < nmz787_i> in the sense that it has to stay open/public but also has a well-known big corporate name attached
10:45 < nmz787_i> i.e. for google hits and credibility or something
10:46 < chris_99> haha, interesting idea
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10:50 < kanzure> credibility is already solved, wont be an issue
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10:55 < nmz787_i> kanzure: I was speaking more generally
10:56 < nmz787_i> but I guess I shouldn't open that until later
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11:06 < kanzure> "Greg Fahy's successful self-experimentation has fast-tracked therapy to regrow the thymus and other organs ... His work is now is the basis of the FDA-approved TRIIM trial."
11:06 < kanzure> didn't know that.
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11:50 < Aurelius_Work2> kanzure : what's that from?
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12:07 < kanzure> grg
12:09 < kanzure> whois luke perrish
12:09 < kanzure> oh nevermind
12:09 < kanzure> i should know that
12:15 < eudoxia_> Oregon cryo person
12:15 < eudoxia_> he follows me on twitter
12:15 < kanzure> nah my question was super embarrassing (it's docl)
12:15 < eudoxia_> yep
12:16 < kanzure> eudoxia_: see PM
12:16 < eudoxia_> ok
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12:33 < docl> well, there goes my anonymity :P
12:33 < kanzure> your github account has your name
12:34 < docl> ah, good point
12:34 < kanzure> just to be safe, you should change your name and burn down the entirety of oregon
12:35 < docl> sounds like a plan :P
12:57 < fenn> basically no information about their therapy: http://interveneimmune.com/?page_id=1131 http://interveneimmune.com/?ignition_product=the-triim-trial
12:58 < kanzure> if you mean fahy stuff, then maybe a patent search would be better
13:03 < kanzure> heh there is a ukrainian translation of drexler's nanosystems book http://softdroid.net/toc
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13:08 < chris_99> https://github.com/jisaacso/DeepHeart
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13:35 < kanzure> hm colwiz's parsers are all broken in lots of ways. they get dates wrong..
13:42 < kanzure> http://engineeringbiologycenter.org/wp-content/uploads/2016/06/HGP-write-Fact-Sheet.pdf
13:43 < kanzure> http://hgpwrite.org/ (seems to be a redirect to http://engineeringbiologycenter.org/ for now)
13:45 < kanzure> http://engineeringbiologycenter.org/faq/
13:47 < kanzure> "Genomically encoded analog memory with precise in vivo DNA writing in living cell populations" http://science.sciencemag.org/content/346/6211/1256272.short
13:48 < kanzure> this technique seems to use recombinases to record things
13:49 < docl> https://beacon-center.org/blog/2016/05/27/evolving-antimutator-microbial-machines/
13:49 < docl> Engineered microbes contain heterologous synthetic devices that utilize a heavy proportion of a cell’s resources for expression. These devices cause cellular stress and decrease fitness, resulting in a strong selection for mutations that eliminate expression of any synthetic part that comprises the device. Over time, random mutations that naturally occur in a living host organism will accumulate and
13:49 < docl> render a synthetic device inactive. Microbes are limited by their inherent unpredictability. This poses a challenging problem for engineers.
13:50 < kanzure> "Genetic encoding of DNA nanostructures and their self-assembly in living bacteria" http://www.nature.com/ncomms/2016/160419/ncomms11179/full/ncomms11179.html
13:50 < kanzure> hm
13:51 < kanzure> beacon-center.org does many good things
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13:53 < kanzure> i thought the usual solution for that problem was something like, "make the synthetic device metabolically relevant to the host organism's survival"
13:53 < kanzure> and terminator genes that execute in its absence.   i suppose you could argue that it will eventually be removed anyway... but meh.
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14:08 < kanzure> andrew back has been refurbishing a scanning electron microscope http://abopen.com/blog/scanning-electron-microscope-fun/
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14:59 < kanzure> typetypetype
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15:17 < nmz787_i> who is andrew back?
15:18 < kanzure> uh just someone in the UK doing open hardware things
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15:36 < kanzure> HEx1: still alive?
15:42 < pasky> any tips re getting hold of
15:42 < pasky> > Experimentation with Human Beings: The Authority of the Investigator,
15:42 < pasky> > Subject, Professions, and State in the Human Experimentation Process, Jay
15:42 < pasky> > Katz, ISBN      1610448340, 9781610448345, 1972
15:42 < pasky> ?
15:44 < nmz787_i> is there a country that excludes people based on intelligence?
15:45 < eudoxia_> nmz787_i, you mean, turns you away at the airport because of an intelligence agency request?
15:45 < nmz787_i> no, as in you need to be smart to be let in
15:45 < nmz787_i> i.e. IQ test is your visa test
15:46 < nmz787_i> if this doesn't exist, would it be a good idea?
15:46 < eudoxia_> hahaha I misread that, not that I know of, but I don't think Singapore does it, and if they don't do it noone else does
15:48 < CaptHindsight> visa test?! They check your credit cards now?
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15:49 < nmz787_i> CaptHindsight: http://www.etymonline.com/index.php?term=visa
15:53 < TMA> nmz787_i: USA (for the non-lottery residence/work-permit visas, the person needs to be qualified)
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15:53 < nmz787_i> TMA: we allow people to be grandfathered in though, so this place isn't starting off fresh
15:55 < TMA> nmz787_i: the only place starting off fresh at the moment is Liberland
15:55 < TMA> nmz787_i: i do not know their policy though
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15:58 < TMA> nmz787_i: the problem is no state or supranational organization acknowledges its existence
16:24 < HEx1> kanzure: yep
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16:25 < HEx1> kanzure: reading the recent enzymaticsynthesis posts is still on my to-do queue :(
16:42 < kanzure> HEx1: we are drafting a review of the concept
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19:14 < CaptHindsight> DNA barcode printer in action  https://ibin.co/w800/2aq0JWfJZfXK.jpg
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20:28 < kanzure> hm.
20:28 < kanzure> "It turns out the brain uses DNA base excision & repair to adjust neurotransmitter levels. This means it inevitably degrades the neural DNA over time. This may be the hardest cause of aging to stop--unless we just turn the mechanism off."
20:29 < kanzure> "Tet3 regulates synaptic transmission and homeostatic plasticity via DNA oxidation and repair" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446239/
20:37 < fenn> is tet3 important?
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21:39 < Aurelius_Home> anyone know of startups that hire based on projects with little to no 'phone technical interview'?
21:52 < kanzure> blockstream, probably :P
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22:01 < nmz787> tet3 sounds like something to do with tetracycline
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22:20 < nmz787> kanzure: "The DNA content of the ocean is doubling every single day."  well, it is probably degrading by half too, no?
22:22 < nmz787> I guess that doesn't matter though
22:43 < nmz787> so that person on DIYbio mentioned tdt low efficiency, but didn't cite anything for me to follow up on and I couldn't understand where he got his magic numbers... and another guy I talked to last week mentioned synchem being higher efficiency than enzymes (I have to go on that guy being of elder age) and that synChem also prevents deletion operations from being extended further (still haven't re-checked the chem on that... but I feel like ...
22:43 < nmz787> ... it is more complex chem that introduces some other issues)
22:45 < nmz787> like an extra capping step or something, that you also have to pull off everytime, but then it like screws up the side-chain information if the chem is too strong
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23:12 < nmz787> kanzure: have you find a purchasing source for fluorescent reversible terminators?
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23:12 < nmz787> FWIW google shopping has lamps or something for those search terms
23:16 < nmz787> hmm, maybe these folks will respond: http://lasergen.com/technology/lightning-terminators/
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23:56 < nmz787> 'contact azco biotech' http://seqanswers.com/forums/showthread.php?t=31555#post109424
23:56 < nmz787> .title
23:56 < yoleaux> where to purchase reversible terminators - SEQanswers
--- Log closed Tue Jul 26 00:00:07 2016