--- Log opened Wed Dec 21 00:00:44 2016
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05:00 < kanzure> bloop
05:02 < darsie> .
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05:36 < cluckj> beep
05:37 < kanzure> have we all taken the obligatory time to admire darth vader's pun in rogue one?
05:38 < cluckj> haven't seen it yet, no spoilers!!
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05:52 < kanzure> bah
06:18 < JayDugger> Star Wars has sucked since its last Leigh Brackett screenplay.
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06:39 < kanzure> musics https://www.youtube.com/watch?v=SoYkxKWNzoo
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11:44 < nmz787> nanostuff in the real-world, talk about a needle in a haystack....
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12:04 < kanzure> .title https://news.ycombinator.com/item?id=13230741
12:04 < yoleaux> Why Are Ultrasound Machines So Expensive? | Hacker News
12:07 < kanzure> hmm "there isn't enough demand for specialty materials fab (PZT thin films for mHz range transducers) to benefit from economies of scale"
12:12 < kanzure> i wonder if it would make economic sense to do a pcb with an oscilloscope and a bunch of other random long-tail weirdo electronics stuff (like ultrasound)
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14:54 < kanzure> .tw https://twitter.com/onetruecathal/status/811648733989535745
14:54 < yoleaux> World's first #BioTech #accelerator SYNBIOAXLR8R/IndieBio EU is now @RebelBio - SynBioBeta - http://synbiobeta.com/news/indiebio-eu-now-rebelbio/ (@onetruecathal)
14:55 < kanzure> https://rebelbio.co/
15:01 < nmz787> i tried going to rebelb.io, but it doesn't work
15:02 < nmz787> wait, wasn't IndieBio Cathal's company?
15:02 < nmz787> hmm, maybe I am misremembering
15:03 < kanzure> well, cathal has some kind of involvement with it, but i don't know if he ever disclosed his role
15:03 < kanzure> *the nature of his role
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15:05 < nmz787> no I was thinking his own startup stuff
15:07 < nmz787> ah, this is his personal site: https://indiebiotech.com/about/
15:07 < nmz787> it adds a suffix of 'tech' to distinguish
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15:20 < kanzure> there really should be a biotech/selection company that does arbitrary directed evolution projects.....
15:20 < kanzure> under the name "bruteforce bio"
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15:50 < nmz787> well I seem to have a model of a nano-sieve with 30nm pores every 120nm in a grid, and some angled sections to allow visual guidance from the macro scale of the 3mm frame
15:50 < nmz787> not to get started on real fluidics :/
15:51 < chris_99> whats that for out of interest?
15:52 < nmz787> next-gen DNA synthesis
15:52 < nmz787> :)
15:53 < nmz787> trying to just get from start to finish of a fab process for this week
15:53 < chris_99> ooh neat
15:54 < nmz787> wanted to work on modelling the nano-sieve I received from a company (which I can't find in real life :/  )
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15:55 < nmz787> I suck at organization
15:55 < chris_99> oh so zeolites are a type of nano-sieve then?
15:55 < nmz787> well, physibles at least
15:56 < nmz787> hmm, yeah in a sense... I mean, it has nano-scale volumes/caves/reaction-chambers/voids
15:56 < nmz787> the thing I got is literally just a sheet of Silicon Nitride with pores/cylinders going through it
15:56 < chris_99> ah interesting
15:57 < nmz787> has a frame of 3mm x 100 microns, with a 500x500 micron window of nanoporous area which itself is 20nm thick
15:57 < nmz787> (3mm square, 100 microns thick)
16:00 < nmz787> interestingly BRLCAD doesn't seem to like to raytrace things below about 3 microns, which might be some setting I have incorrectly adjusted... but its easy enough to just tell it I'm using millimeters instead of microns and things seem to work alright
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16:00 < chris_99> odd
16:03 < chris_99> will you need lots of expensive chemicals for synthesis though?
16:08 < nmz787> http://imgur.com/a/BOgKZ
16:09 < nmz787> you can (or can't) see in the first image how it looks like nothing is there
16:09 < nmz787> that's why on the backside, I made the rectangular funnel cutout, to guide you visually down to the nano-action
16:10 < nmz787> chris_99: possible, but hoping to use enzymes to supplant the more sensitive/expensive stuff
16:10 < chris_99> ah
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17:48 < nmz787> pretty good overview of bunch of valve types: https://pdfs.semanticscholar.org/f03a/b20ecee093c84e9451b082321cabd0e845c2.pdf
17:48 < nmz787> .title
17:48 < yoleaux> nmz787: Sorry, that doesn't appear to be an HTML page.
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19:15 < kanzure> "A highly sensitive selection method for directed evolution of homing endonucleases" http://nar.oxfordjournals.org/content/33/18/e154.short
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19:35 < kanzure> "Why a particle physicist is interested in DNA branch migration" https://arxiv.org/abs/hep-lat/9608111
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19:49 < kanzure> "Single molecule techniques in DNA repair: A primer" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125534/
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20:24 < kanzure> "Construction of multifragment plasmids by homologous recombination in yeast" http://www.jsvanleeuwen.com/inc/pdf/van_Leeuwen_2015_CSHP_intro.pdf
20:24 < kanzure> "One of the first applications of homologous recombination in yeast for the construction of plasmids from DNA fragments was described by the Botstein laboratory (Ma et al. 1987). In these early experiments, large homology regions of several-hundred base pairs were used. However, it quickly became clear that as little as 30- to 50-bp homology regions are sufficient to induce recombination, whic...
20:25 < kanzure> ...h opened the door for PCR-mediated strategies in which homology regions are introduced in the primers (Fig. 1B) (Baudin et al. 1993; Manivasakam et al. 1995; Hua et al. 1997; Oldenburg et al. 1997)"
20:28 < kanzure> "If a low recombination rate is anticipated, for example owing to the low concentration or large size of an insert, loss of a (counter)selectable marker in the vector can be used to screen for recombinants among the resulting transformants (Ma et al. 1987; Gunyuzlu et al. 2001; Noskov et al. 2002; Raymond et al. 2002; Kitazono 2009)."
20:28 < kanzure> man those are some disturbingly recent references.....
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22:30 <@jrayhawk> https://www.youtube.com/watch?v=G92kHgieiKk Art De Vany rambling about aging for an hour
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22:42 < nmz787> grrr, which valve to choose an on-chip pump for a stupid 'hello-world' pump-colored-water-around-some-letter-shaped-channels
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23:16 < jcorgan> why, just use the pump-colored-water-around-some-letter-shaped-channels-stupid-hellow-world pump, duh
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--- Log closed Thu Dec 22 00:00:45 2016