--- Log opened Fri Feb 10 00:00:29 2017
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00:54 < nmz787> .title http://www.nature.com/nphys/journal/v6/n6/full/nphys1637.html
00:54 < yoleaux> Integrated elastomeric components for autonomous regulation of sequential and oscillatory flow switching in microfluidic devices : Nature Physics : Nature Research
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01:09 < nmz787> .title http://pubs.acs.org/doi/abs/10.1021/nl304063f
01:10 < yoleaux> nmz787: Sorry, that doesn't appear to be an HTML page.
01:10 < nmz787> Nanoscale Squeezing in Elastomeric Nanochannels for Single Chromatin Linearization
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01:25 < nmz787> http://domino.research.ibm.com/library/cyberdig.nsf/papers/6482D3B7D94062B785257384005D2882/$File/rc24242.pdf
01:25 < nmz787> DNA Transistor
01:28 < nmz787> .title http://aip.scitation.org/doi/10.1063/1.2798247
01:28 < nmz787> Nanopore in metal-dielectric sandwich for DNA position control
01:42 < nmz787> .title http://ieeexplore.ieee.org/document/6724627/
01:42 < nmz787> .title
01:42 < nmz787> 200 mm wafer-scale integration of sub-20 nm sacrificial nanofluidic channels for manipulating and imaging single DNA molecules
01:42 < nmz787> http://www.nature.com/articles/ncomms14243
01:42 < nmz787> Wafer-scale integration of sacrificial nanofluidic chips for detecting and manipulating single DNA molecules
01:43 < yoleaux> nmz787: Sorry, that command (.title) took too long to process.
01:43 < yoleaux> nmz787: Sorry, that command (.title) took too long to process.
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02:01 < eb3c90> kanzure: Elon is basically of the position that for safe super intelligence the best bet is to connect AGI with a high bandwidth connection to humans and spread them throughout humanity (so no group goes insane with power etc)
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02:36 < archels> communism!
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02:46 < eb3c90> I'm not sure that you need the high bandwidth connection. You just need the IA to have the correct type of connection. But high bandwidth is better!
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02:53 < nmz787_w> IA == Internet Archive?
02:53 < eb3c90> Intelligence Augmentation
02:53 < nmz787_w> the other alternative I thought of was Intel Architecture
02:54 < eb3c90> My take on it is that it is a bit like AI, but you are not trying to create an entity with own goals
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04:45 < kanzure> Lemminkainen: the cheap genome stuff? that was just some other topics that fit the presentation theme.
04:53 < rkos> is the minion really worth 1k btw, it was 1k many years ago and the price hasnt come down at all?
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04:58 < rkos> is it just too soon for hobbyist sequencing yet or is it viable?
05:00 < chris_99> isn't it single use or something
05:01 < rkos> well yea i think so like not the device but some parts of it are single use and need to be replaced for some equally steep price
05:01 < chris_99> ah
05:02 < rkos> but i would only sequence myself with it anyways
05:03 < rkos> but i have absolutely no training or anything so im dubious its doable
05:03 < rkos> https://store.nanoporetech.com/minion/sets
05:03 < chris_99> eek
05:04 < rkos> i remember it coming out like 2012 2011? seems still to be the same price
05:05 < rkos> upcoming they have the multiuse device but thats like 70k and only for licensed professionals too
05:07 < rkos> but i like the idea of sequencing myself by myself without giving out the data for marketing research
05:07 < rkos> or such
05:07 < chris_99> mm
05:09 < rkos> and generally itd seem like a fun activity
05:10 < rkos> rather than just ordering some kit and mailing it somewhere to hear the divinations of some company
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05:11 < rkos> id just want to have the sequence somewhere on file mby figure out some point for having it at a later date, maybe not, but regardless ive wanted to do it for years and years, noone here have any suggestions?
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05:12 < chris_99> i don't know anything about sequencing the human genome, are those minion devices as 'good' as bigger machines
05:12 < rkos> im sure theyre not
05:13 < rkos> https://en.wikipedia.org/wiki/Nanopore_sequencing#Challenges
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05:18 < rkos> well the 23andme thing would be a lot cheaper but it seems a bit sketchy, they seem to use illumina devices tho which seems nice
05:20 < rkos> cant someone in china do it even cheaper
05:20 < rkos> dont they have like big halls filled with newest illumina gadgets
05:22 < streety> my understandng is that they started off with a very high error rate but that has dropped a lot with the ongoing development
05:23 < streety> probably how they justify the price staying the same
05:23 < streety> if they even bother, their target market probably isn't that price sensitive
05:23 < streety> certainly not on the actual device, probably more so on the reagents
05:28 < chris_99> can you reduce error rate by sequencing a number of times
05:30 < streety> if the error rate is low enough sure. I think in the beginning the error rate was high enough that it was difficult to be sufficiently confident in the alignment to get error correction by consensus
05:30 < streety> this is all second hand understanding though
05:31 < streety> and it does seem to have improved
05:31 < chris_99> ahh interesting
05:32 < rkos> seem to be a lot of companies offering differing services http://isogg.org/wiki/List_of_personal_genomics_companies
05:35 < abetusk> Also http://arep.med.harvard.edu/gmc/genome_services.html
05:40 < streety> genomix is a fifth the price of any of the others. Is the only difference CLIA certification?
05:41 < rkos> whats clia certification
05:41 < rkos> thanks for the link ill browse these too
05:43 < abetusk> I'm not sure genomix is real
05:44 < rkos> it says they are working on it and it dont seem real at least yet
05:45 < rkos> i like the full genomes site its old fashioned
05:46 < streety> yeah, looking at genomix $200 seems to be a target for late 2017
05:46 < streety> uncertain whether that would actually happen
05:47 < streety> CLIA is a quality assurance organization/set of standards
05:48 < streety> where you might look for a CE mark on an electronic device any lab doing medical diagnostics would have CLIA certification
05:49 < rkos> is there no darknet genomics lab lol
05:49 < streety> not to say a lab without CLIA certification is going to be bad necessarily or a lab with certification isn't going to mess up
05:51 < abetusk> It's only a recent event that whole genome sequencing got to the $1k price point.  I don't think CLIA certification is the cost barrier, I think the technology is
05:51 < rkos> well ill make no hasty moves and sit on the idea still some more but the fullgenomes one seems interesting
05:51 < rkos> https://www.fullgenomes.com/qanda/
05:52 < rkos> they seem to have a much more sensible privacy policy than eg 23andme
05:57 < kanzure> also check scienceexchange
06:08 < rkos> ok seems interesting tho i think im starting to be overloaded with this browsin and gonna have to distract myself with something else for a while
06:08 < rkos> wheres the decentralized cryptocurrency sciencemarket lol
06:10 < kanzure> .wik beggars in spain
06:10 < yoleaux> "Beggars in Spain is a 1993 science fiction novel by Nancy Kress. It was originally published as a novella with the same title in Isaac Asimov's Science Fiction Magazine and as a limited edition paperback by Axolotl Press in 1991." -- https://en.wikipedia.org/wiki/Beggars_in_spain
06:11 < kanzure> ( https://www.reddit.com/r/AskReddit/comments/5t2d70/scientists_have_discovered_a_way_to_make_sleeping/ddkgv3g/ )
06:12 < rkos> fatal familial insomnia is an interesting disease
06:15 < kanzure> or DEC2 mutation thing https://www.scientificamerican.com/article/genetic-mutation-sleep-less/
06:25 < kanzure> "In the late 2000s, a mouse model was made for FFI. These mice expressed a humanized version of the PrP protein that also contains the D178N FFI mutation.[15] These mice appear to have progressively fewer and shorter periods of uninterrupted sleep, damage in the thalamus, and early deaths, similar to humans with FFI."
06:25 < kanzure> cool.
06:25 < kanzure> "As of 2016, studies suggest that doxycycline may be able to slow or even prevent the development of the disease.[16][17][18]"
06:25 < kanzure> .ik doxycycline
06:25 < kanzure> .ik doxycycline
06:25 < kanzure> .wik doxycycline
06:25 < yoleaux> "Doxycycline is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. It is useful for bacterial pneumonia, acne, chlamydia infections, early Lyme disease, cholera and syphilis. It is also useful for the treatment of malaria when used with quinine and for the prevention of malaria." -- https://en.wikipedia.org/wiki/Doxycycline
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07:41 < kanzure> .tw https://twitter.com/VesselOfSpirit/status/825904523407355906
07:41 < yoleaux> humans sleep because evolution discovered that turning you off and then on again was the easiest fix (@VesselOfSpirit)
08:23 < fenn> beggars in spain is a very political book
08:24 < kanzure> should avoid?
08:24 < fenn> well i'm just warning that it's mostly not about sleeping and mostly about people whining about other people
08:24 < fenn> it was an ok book series
08:25 < kanzure> if i want to whine about people then i can just get on irc
08:26 < docl> wasn't there also a space station in that book somewhere?
08:29 < fenn> yes
08:30 < kanzure> "biohack the planet 2017 call for talks https://goo.gl/forms/k2VSvzYfcDcdKAUL2 Tentative dates are July 21, 22 in Oakland, CA" http://biohacktheplanet.com/
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08:35 < fenn> the book's depiction of a welfare state was interesting
08:35 < kanzure> if ted chiang got a movie made, does that mean he has funding now?
08:36 < fenn> what movie?
08:36 < kanzure> https://en.wikipedia.org/wiki/Arrival_%28film%29
08:39 < fenn> illeneuve ended up changing the title, partly because the resulting script became so far removed from the short story, as well as sounding "more like a romantic comedy"
08:39 < fenn> uh huh
08:44 < kanzure> music https://www.youtube.com/watch?v=YWGq4Z-W9CY
09:02 < kanzure> delinquentme says that microsoft has contract with twist to do dna archival like https://catalogdna.com/ but i dunno why
09:03 < kanzure> it's going to be write-only, and you'd have better read-write with tape memory....
09:03 < kanzure> if you want to store lots of data that you're almost never going to read, i guess DNA makes sense if you have super cheap write.... which you don't.
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09:41 < kanzure> .wik hyperthymesia
09:41 < yoleaux> "Hyperthymesia is the condition of possessing an extremely detailed autobiographical memory. People with hyperthymesia remember an abnormally vast number of their life experiences." -- https://en.wikipedia.org/wiki/Hyperthymesia
09:48 < JayDugger> Omits Severian, from Gene Wolfe's fiction, in its "Appearance in fiction" section.
10:12 < kanzure> nobody knows about gene wolfe anyway
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11:47 < kanzure> qer0328ht20ada
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13:27 < kanzure> https://cosmicnootropic.com/
13:28 < kanzure> ooh cerebrolysin
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14:03 < kanzure> http://www.gizmodo.co.uk/2017/02/germany-is-threatening-biohackers-with-prison/
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14:09 < kanzure> https://www.meetup.com/Renaissance-Bio/events/236993383/
14:09 < kanzure> epigenetic clock ant-aging stuff livestream, 5pm PST https://hangouts.google.com/call/jbycf3q4vzcvhjtyiq6vsxalkqe
14:09 < maaku> http://physics.stackexchange.com/questions/20813/how-would-a-black-hole-power-plant-work
14:10 < maaku> how have I gone this long without knowing about black hold power plants and hawking radiation engines...
14:10 < kanzure> "The presenters are Dr. Steve Horvath, one of the primary discovers/developers of the epigenetic clock, and Dr. James P. Watson, a physician and co-author of the Anti-Aging Firewalls blog."
14:10 < kanzure> maaku: also there are black hole engines and black hole computers
14:11 < kanzure> maaku: http://diyhpl.us/~bryan/papers2/physics/astrophysics/black-holes/
14:21 < kanzure> 14:04 <+kanzure> http://www.gizmodo.co.uk/2017/02/germany-is-threatening-biohackers-with-prison/
14:21 < kanzure> 14:06 <+sbp> real news: "Germany is threatening prisons with biohackers"
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15:32 < docl> maaku: have you seen this yet? https://en.wikipedia.org/wiki/Black_hole_starship
15:39 < cluckj> kanzure, how was your conference?
15:39 < kanzure> talk went fine, it was just me no conference
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15:53 < cluckj> ah
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17:05 < streety> what have the prisons done?
17:05 < streety> RE: "Germany is threatening prisons with biohackers"
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17:42 < cluckj> hahaha
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19:20 < kanzure> "High-resolution imaging of cellular dopamine efflux using a fluorescent nanosensor array" http://www.pnas.org/content/early/2017/02/07/1613541114.abstract
19:21 < kanzure> "Multidimensional encoding of brain connectomes" http://biorxiv.org/content/early/2017/02/10/107607
19:21 < kanzure> "Using high-throughput barcode sequencing to efficiently map connectomes" http://biorxiv.org/content/early/2017/02/09/099093
19:21 < kanzure> i guess they reposted the synseq paper?
19:24 < kanzure> "Engineered shell proteins confer improved encapsulated pathway behavior in a bacterial microcompartment" http://biorxiv.org/content/early/2017/02/07/106716
19:26 < kanzure> "An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells" http://biorxiv.org/content/early/2017/02/05/106112
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19:29 < kanzure> "Here, we constructed a CRISPR-ON system to efficiently upregulate endogenous genes in hPSCs. A doxycycline (Dox) inducible dCas9-VP64-p65-Rta (dCas9-VPR) transcription activator and a reverse Tet transactivator (rtTA) expression cassette were knocked into the two alleles of the AAVS1 locus to generate an iVPR hESC line. We showed that the dCas9-VPR level could be precisely and reversibly cont...
19:29 < kanzure> ...rolled by the addition and withdrawal of Dox. Upon transfection of multiplexed gRNA plasmid targeting the NANOG promoter and Dox induction, we were able to control NANOG gene expression from its endogenous locus. Interestingly, an elevated NANOG level promoted naive pluripotent gene expression, enhanced cell survival and clonogenicity, and enabled hESCs to integrate with the inner cell mass (I...
19:29 < kanzure> ...CM) of mouse blastocysts in vitro. Thus, iVPR cells provide a convenient platform for gene function studies as well as high-throughput screens in hPSCs."
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19:37 < kanzure> anti-aging firewalls webinar link was wrong :( should have been https://hangouts.google.com/call/5eggkrvozrc3jhaiaxjkeycvzue
19:44 < kanzure> you guys are boring
20:06 < juri_> yep.
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--- Log closed Sat Feb 11 00:00:30 2017