--- Log opened Wed Mar 15 00:00:04 2017
--- Day changed Wed Mar 15 2017
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00:09 < nmz787_> whoops, forgot to turn on a timer, that would be why it wasn't working
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04:05 < kanzure> nmz787_: did you also confirm that it's not simply natural competence of the ecoli?
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04:22 < archels> https://www.youtube.com/watch?v=ALBWxGik64A
04:22 < archels> .title
04:22 < yoleaux> Experimenting with Danger - YouTube
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05:29 < streety> nmz787_: high school was a while ago but I don't think we had any sort of stone counter tops
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05:30 < streety> In fact I think it may have just been wood with a varnish like coating
05:30 < streety> research labs I've been in have been formica
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05:31 < streety> at uni undergrad, uni research labs and gov research labs
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05:32 < streety> steel in animal housing facilities, though that is a very small sample
05:34 < streety> that's mainly the biomedical field though. Only been in one chemistry undergrad teaching lab and one chemsitry research lab. That was mass spec focused and seemed to have been buit up as they went from what was available
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07:25 < kanzure> .title https://news.ycombinator.com/item?id=13872670
07:25 < yoleaux> Introducing Keras 2 | Hacker News
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09:51 < kanzure> .title https://news.ycombinator.com/item?id=13874559
09:51 < yoleaux> BeagleBone Blue - A complete, Linux-enabled robotics computer | Hacker News
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10:38 < nmz787_i> kanzure: competence not checked, but I also didn't transform anything... just cooked some food for the bacteria, some had antibiotic, some didn't... I didn't check that the supposedly-non-resistant bacteria couldn't grow on the antibiotic food
10:44 < nmz787_i> kanzure: I mean I cooked them some food, then scraped some from the agar plate I received on in the mail, and put them on the new food
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11:45 < kanzure> .title https://news.ycombinator.com/item?id=13877844
11:45 < yoleaux> Genetic variant accelerates normal brain aging in older people by up to 12 years | Hacker News
11:45 < kanzure> (rs3173615)
11:58 < nmz787_i> 'life finds a way' https://media.giphy.com/media/VHW0X0GEQQjiU/giphy.gif
11:59 < nmz787_i> (me thinking of preparing sterile plates and hoping they stay sterile for very long)
12:05 < nmz787_i> just ordered another section of matching granite countertop... gonna build another 6 ft of counter with a shelf underneath.
12:05 < nmz787_i> I might want to think about how to make the woodwork a bit less slapped-together
12:06 < nmz787_i> 'look' less slapped-together... structurally they're fine
12:15 < chris_99> do you have a piccy of how the woodwork looks atm?
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13:14 < nmz787_i> chris_99: yeah, one sec
13:15 < nmz787_i> this was during construction http://imgur.com/lLjgREq
13:15 < nmz787_i> and here is pretty much as-is https://groups.google.com/d/msg/diybio/efDDosYfqyg/k4QnGQnVBAAJ
13:16 < nmz787_i> you can see a bit of a door-frame on the right side of the last pic... I want to add a bench on the right side of that door (in this same room)
13:19 < chris_99> cool nice :)
13:19 < chris_99> i was gonna ask if some support between the legs might help, but in the 2nd photo theres a shelf type thing on it too
13:20 < nmz787_i> yeah, another band of 2x4s down there, with a piece of plywood on top for a shelf
13:21 < nmz787_i> the front-side 2x4 below the shelf is horizontal, so it didn't take as much height from sliding things under neath
13:32 < nmz787_i> now I've gotta figure out my plan for lysis and plasmid cleanup and transformation
13:39 < nmz787_i> just confirmed these kits contain everything needed to start from a cell-pellet: http://www.epochlifescience.com/Product/PurificationKit/dna_mini.aspx
13:40 < nmz787_i> hmm, they also offer synthesis
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15:57 < streety> that looks good nmz787_i I don't think it needs anything further to avoid looking slapped together
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16:10 < nmz787_i> streety: thanks :)  not to mention I might criticize myself for it not matching the first one... and it wouldn't match the rest of the cabinets in that room anyway... since I don't have that much time and energy to devote to woodwork right now
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16:54 < streety> nothing worse than unmatched furniture :)
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18:47 < nmz787_> hmm, I have agarose and running buffer, but no gel electrophoresis tray/etc... I wonder if I can cobble together some kind of microfluidic electrophoresis thing by next week :/
18:50 < kanzure> is this all an elaborate ploy to get me to buy you a full molecular biology lab
18:50 < kanzure> i think there was a $1k kit on the-odin.com for that
18:55 < nmz787_> haha
18:56 < nmz787_> I see some gel boxes on ebay for 75-125... but I have always thought that was just too much for what it is
18:56 < nmz787_> I was just looking at that $1k kit
18:58 < nmz787_> you should only buy me enough, such that I am forced to build the rest of my toolset
18:59 < nmz787_> but not that $1k kit
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19:07 < cluckj> nmz787_, I think avery at bosslab has schematics for a diy gelbox
19:10 < nmz787_> ah, cheers, will take a look
19:10 < nmz787_> i had ideas years ago of vacuum-forming one with plastic picnic plates
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19:12 < cluckj> iirc his "needed" a laser cutter, but you can probably wing it without one?
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20:21 < kanzure> blooop
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23:22 < nmz787_> http://justinpiggy.me/index.php/2016/06/12/tcd1304-verilog-driver-for-spectrometer-project/
23:22 < nmz787_> .title
23:22 < yoleaux> TCD1304 Verilog Driver for Spectrometer Project - Justin's Blog
23:23 < nmz787_> http://justinpiggy.me/index.php/2017/01/24/ccd-driver-implemented-on-zynq7000/
23:24 < nmz787_> .title
23:24 < yoleaux> CCD driver implemented on ZYNQ7000 - Justin's Blog
23:24 < nmz787_> .tell chris_99 you had a zynq right? maybe checkout that link ^
23:24 < yoleaux> nmz787_: I'll pass your message to chris_99.
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--- Log closed Thu Mar 16 00:00:59 2017