--- Log opened Mon Mar 20 00:00:03 2017
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05:09 < kanzure> "A virtual world space agency" https://www.academia.edu/2700115/A_Virtual_World_Space_Agency
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06:37 < kanzure> "Using emulsion droplets as reactors for assembling nanoparticles" https://twitter.com/ChockyNikita/status/843786096492396549
06:37 < kanzure> "Molecular events within a confined space" https://twitter.com/donmman/status/843784414287028227
06:39 < kanzure> "Branched polymerization systems for nanoemulsion drug delivery" https://twitter.com/stephyellen/status/843762457416032256
06:40 < kanzure> "Low-cost high-throughput screening of all inorganic compounds" https://twitter.com/danwdavies/status/843753109717471232
06:42 < kanzure> lotta posters today on the twitters https://twitter.com/hashtag/RSCPoster
06:46 < kanzure> .tw https://twitter.com/readcoor/status/843480529366384640
06:46 < yoleaux> In order to explore the brain more fully, IARPA's MICRoNS was awarded $21M to map 1 cubic mm of cortex #BrainAwarenessWeek (@readcoor, in reply to tw:842831982488944640)
06:46 < kanzure> .tw https://twitter.com/readcoor/status/843481156020572160
06:46 < yoleaux> MICRoNS enables the creation of the first neural circuit synaptic level map with optical microscopy #BrainAwarenessWeek (@readcoor, in reply to tw:842831982488944640)
06:46 < kanzure> so 500k neurons for $21 million dollars?
06:49 < kanzure> "A dye-assisted paper-based point-of-care assay for fast and reliable blood grouping" http://stm.sciencemag.org/content/9/381/eaaf9209
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08:50 < kanzure> $42/neuron
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11:13 < archels> .title http://theconversation.com/a-hot-bath-has-benefits-similar-to-exercise-74600
11:13 < yoleaux> A hot bath has benefits similar to exercise
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11:47 < nmz787_i> archels: thanks, I think I can manage to laptop in the bath
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12:01 < kanzure> we're counting on you
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12:12 < entity8421[m]> its so bad that human head is not multiprocess
12:48 < nmz787_i> entity8421[m]: I have multiprocess, but only for certain tasks
12:48 < entity8421[m]> for choosing women i suppose
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12:53 < nmz787_i> entity8421[m]: more obviously for things like driving
12:56 < entity8421[m]> Excursion Funnel.mp3
12:56 < entity8421[m]> could be there even better "towards singularity" background music
12:57 < entity8421[m]> Portal_2_OST_Excursion_Funnel.mp3
12:58 < entity8421[m]> (uploaded Portal_2_OST_Excursion_Funnel mp3 here)
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16:08 < kanzure> bloop
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16:54 < kanzure> Lindqvist PG, Epstein E, Nielsen K, Landin-Olsson M, Ingvar C, Olsson H. Avoidance of sun exposure as a risk factor for major causes of death: a competing risk analysis of the Melanoma in Southern Sweden cohort. J Intern Med. 2016 Oct;280(4):375-87
16:54 < kanzure> Lindqvist PG, Epstein E, Landin-Olsson M, Ingvar C, Nielsen K, Stenbeck M, Olsson H. Avoidance of sun exposure is a risk factor for all-cause mortality: results from the Melanoma in Southern Sweden cohort. J Intern Med. 2014 Jul;276(1):77-86
17:51 < kanzure> anyone want to answer this one? https://github.com/kanzure/papermonk/issues/2
18:16 < kanzure> nevermind.
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19:08 < kanzure> yashgaroth: sup?
19:08 < yashgaroth> not much
19:10 < kanzure> yashgaroth: here are some motorcycles https://www.youtube.com/watch?v=g02VBsfNuSU
19:11 < yashgaroth> alright cool; oh I was looking at lipocalin 2 after this paper came out http://www.nature.com/nature/journal/v543/n7645/full/nature21697.html
19:11 < kanzure> .title http://www.nature.com/nature/journal/v543/n7645/full/nature21697.html
19:11 < yoleaux> MC4R-dependent suppression of appetite by bone-derived lipocalin 2 : Nature : Nature Research
19:11 < kanzure> .wik lipocalin 2
19:11 < yoleaux> "Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), is a protein that in humans is encoded by the LCN2 gene. NGAL is involved in innate immunity by sequestrating iron that in turn limits bacterial growth." -- https://en.wikipedia.org/wiki/Lipocalin_2
19:12 < yashgaroth> it does a bunch of stuff, which makes me cautious about injection, but seems like it'd be pretty easy to make in e.coli
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20:24 < nmz787_> yashgaroth: got some supplies from the-odin... they didn't have a lux plasmid to send, said their culture got contaminated
20:24 < yashgaroth> a likely story
20:24 < nmz787_> not sure if they will ressurect it and send me some
20:25 < nmz787_> I got some GFP instead though
20:25 < nmz787_> waiting on some spin columns from epoch labs now
20:25 < nmz787_> I gotta sterilize some pipette tips, should I just wrap them in foil and bake them at 121 C?
20:26 < nmz787_> or 125?
20:26 < nmz787_> (the whole box)
20:26 < yashgaroth> you don't have a pressure cooker? they're great for beans
20:26 < nmz787_> yeah I have 3
20:26 < nmz787_> but I thought these are 'dry cycle'
20:27 < yashgaroth> well if you can get an oven to exactly 121C then do it, but tips'll get brittle at much higher than that
20:27 < nmz787_> I only have a standoff thing for the big-ass canner
20:27 < nmz787_> oh
20:27 < nmz787_> hmm
20:27 < nmz787_> it has a convection fan in it
20:28 < nmz787_> 121 C === 249.8 F... so I'd guess it should be able to handle 250F +- a degree or two
20:29 < yashgaroth> I've never been cheap enough to autoclave tips, though admittedly I'm living off surplus tips from previous employers
20:31 < yashgaroth> but yeah 250F oven should be okay since they're single use
20:31 < nmz787_> some stuff I'm reading says wet cook them, then dry at like 60 C in oven for 2 hours
20:31 < nmz787_> I mean pressure cook them
20:32 < yashgaroth> should work too, the thing about dry cooking is it takes a lot longer to get the same sterilization
20:34 < yashgaroth> either way, whichever's easier...unless you're gonna use them in a BSC, they'll be adequately sterile
20:48 < nmz787_> BSC?
20:48 < yashgaroth> biosafety cabinet
20:48 < nmz787_> I threw them in foil and into a medium sized pressure cooker with a brocolli cooker below as a standoff
20:48 < nmz787_> will dry them after that
20:49 < nmz787_> I won't get the columns for another two days
20:49 < nmz787_> yashgaroth: is ampicilling 1000X stock OK if left out at 23 C?
20:49 < nmz787_> ampicillin*
20:49 < nmz787_> I made it up last week, then left it out since then
20:50 < nmz787_> after using 30mL agar worth
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20:52 < yashgaroth> depends on what it's in
20:55 < yashgaroth> that's one reason I prefer kanamycin; just keep it in aliquots in the freezer if you can
20:56 < yashgaroth> anyway yeah it's probably fine if you just dissolved it in water
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20:59 < nmz787_> yeah it was distilled from a grocery store
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--- Log closed Tue Mar 21 00:00:04 2017