--- Log opened Wed Mar 22 00:00:05 2017
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04:07 < nmz787_> http://i.imgur.com/iZl8iQh.gif
04:09 < nmz787_> a PWM period of  3200, decrementing to 100, in steps of 100... with the Yellow trace being the High-Voltage
04:13 < archels> hey, I didn't know those Rigol scopes could save animations to file. neat
04:18 < kanzure> hm.
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04:25 < fenn> that's all it takes to get into YC these days? that shipping container looks like garbage, sorry
04:26 < fenn> and "rent out shipping containers in your yard" has more than a couple problems as a business model
04:36 < archels> kanzure: any more info on volthealth.com?
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07:22 < kanzure> archels: no.
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07:46 < kanzure> "Survival from a lethal blood concentration of cyanide with associated alcohol intoxication" http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2044.2000.01628.x/full
07:56 < chris_99> intriguing
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10:09 < nmz787_> archels: they can't... I just had a 6 second delay between each pulse, and manually hit the 'print screen' button, which saves a PNG to file
10:09 < archels> haha, neat
10:10 < archels> I don't know exactly what you're trying to do but the bottom waveform looks terrible D:
10:14 < nmz787_> archels: that is kilovolts, seen through a gigaohm resistor
10:15 < nmz787_> archels: transistors pulse the low-side of a coil, causing high-voltage inductive spikes on the high-side of the coil... which are capture by diodes and caps, and then fed out to the probe
10:15 < nmz787_> archels: purpose is to zap bacterial with the high voltage, to get them to open their sweet, sweet, membranes
10:16 < archels> haha
10:16 < archels> do they prefer DC or pulsed DC?
10:16 < archels> right now there's a lot of high frequency pulses riding on top of the bulk DC value
10:17 < nmz787_> seems they mostly just care about the average field strength
10:17 < nmz787_> AC also works
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10:17 < nmz787_> the recipes are all organism dependent though
10:17 < archels> hm, strange
10:18 < archels> in the neuronal world, static voltage vs dV/dt makes a world of difference
10:18 < nmz787_> yeah, that's more of a data transmission realm though
10:20 < archels> well, the main goal there is to induce a current across the lipid bilayer
10:20 < nmz787_> while this is twisting all the polar molecules, especially the lipid bilayer, which causes holes to form (and also DNA is electrophoresis, and may flow into said hole)
10:20 < kanzure> electrophoretic activity
10:20 < nmz787_> so I think as long as you get over some threshold, things are probably ok
10:20 < archels> ah, wow, that's the mechanism? very interesting
10:21 < nmz787_> electrophoresing*
10:21 < nmz787_> yeah
10:21 < kanzure> electrophorescing
10:21 < kanzure> (i'm making shit up. don't mind me.)
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10:23 < nmz787_> archels: http://www.cell.com.sci-hub.cc/biophysj/abstract/S0006-3495(90)82348-1
10:24 < nmz787_> archels: I will ask about how to smooth the output a bit more though, it is probably relevant to physics ;)
10:26 < archels> capacitors. big capacitors
10:26 < nmz787_> g2g work on documentation for some shit I was compiling at work... ttyl
10:26 < archels> ~
10:26 < nmz787_> archels: getting away from the requirement for big capacitors was actually the inspiration for this circuit
10:29 < archels> then you might need a higher pulse frequency
10:29 < archels> sorry, I lost the link to your github
10:30 < archels> nevermind--got it
10:34 < archels> hmm, what's the purpose of C1 and C2 on the secondary? https://raw.githubusercontent.com/nmz787/culture_shock/master/kvboard.png
10:34 < archels> oh, it's a Cockroft-Walton voltage doubler, isn't it
10:34 < archels> single stage
10:36 < archels> one thing that is potentially weird about the way you're driving the circuit is that the frequency of the pulses changes, but not the duty cycle
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10:46 < nmz787_i> archels: D1 and D2 and C4 seem to contribute to a second stage of the doubler too
10:47 < nmz787_i> archels: I can also change the duty cycle... I just didn't for that vid, as the pulse width has a reasonably large effect on the output voltage
10:48 < nmz787_i> I did notice there was no audible noise at the higher freqs, which I thought was less worrisome sounding... but also less indicative... so I could go either way on a final method
10:49 < nmz787_i> I can try a video tonight where I hold the period and only adjust the width
10:50 < nmz787_i> my scope has ethernet too... and I think with that I can setup a completely automated script, such that I could make a shmoo plot
10:50 < nmz787_i> but I also don't want to over-voltage anything and break it before I actually test it with bacteria
10:50 < archels> hehe
10:51 < archels> yeah, there's no overvoltage clamp
10:51 < archels> you could set it to just shutdown completely upon overvoltage detection on the feedback line
10:53 < nmz787_i> well that feedback line goes to a GPIO that can be muxed with the ADC... so it would take potentially-buggy code, or maybe some sort of non-microcontroller circuitry
10:53 < nmz787_i> ADC is next on my plan
10:53 < archels> you could hook it up to an analog comparator temporarily
10:53 < nmz787_i> mm, yeah
10:54 < nmz787_i> that would be a lot more 'sure' IMO
10:54 < nmz787_i> until the calibration goes out :P
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14:34 < entity8421[m]> Is there a self-hosted (or free), hierarchical note taking web/desktop app like CherryTree, but with networking and encrypted? (so others can edit same document)
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15:07 < superkuh> ssh, folders, text files.
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18:48 < kanzure> "Deep brain fluorescence imaging with minimally invasive ultra-thin optical fibers" https://arxiv.org/abs/1703.07633
18:50 < kanzure> "The blood DNA virome in 8,000 humans" http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006292 https://twitter.com/NatureRevMicro/status/844682681703157765
18:54 < kanzure> "Two-photon calcium imaging of medial prefrontal cortex and hippocampus without cortical invasion" http://biorxiv.org/content/early/2017/03/22/119404 https://twitter.com/biorxiv_neursci/status/844639968882573312
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19:03 < kanzure> "Light sheet theta microscopy for high-resolution quantitative imaging of large biological systems" http://biorxiv.org/content/early/2017/03/22/119289 https://twitter.com/biorxiv_neursci/status/844579604455198721
19:07 < kanzure> "Deep LSTM for large vocabulary continuous speech recognition" https://arxiv.org/abs/1703.07090 https://twitter.com/arxiv_cscl/status/844544338810093569
19:10 < kanzure> .tw https://twitter.com/MATscientists/status/844517637870768128
19:10 < yoleaux> Brilliant! I wonder if my 4-year old twins can build a LEGO-based uCT scanner for my lab? #innovation #scienceed https://twitter.com/PLOSBiology/status/844516342715830273 (@MATscientists)
19:10 < kanzure> good ol' child labor :)
19:11 < kanzure> .tw https://twitter.com/CellStemCell/status/844495974437392384
19:11 < yoleaux> George Church sparking a discussion about whether the 14-day rule should still apply. https://twitter.com/rosario_isasi/status/844198720413618178 (@CellStemCell)
19:14 < kanzure> annnd my tab crashed.
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--- Log closed Thu Mar 23 00:00:06 2017