--- Log opened Tue May 16 00:00:55 2017 00:00 -!- pompolic [~A@unaffiliated/pompolic] has quit [Ping timeout: 240 seconds] 00:11 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 00:32 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 00:32 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 00:32 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 00:39 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 00:41 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 246 seconds] 00:47 -!- sachy [~sachy@77.87.241.77] has quit [Ping timeout: 240 seconds] 00:48 -!- justan0theruser is now known as justanotheruser 01:04 -!- preview [~preview@2407:7000:842d:4000::3] has joined ##hplusroadmap 01:05 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Quit: Leaving.] 01:07 -!- preview_ [~preview@2407:7000:842d:4000::3] has joined ##hplusroadmap 01:07 -!- preview [~preview@2407:7000:842d:4000::3] has quit [Read error: Connection reset by peer] 01:12 -!- augur [~augur@2601:640:8001:4222:35af:6628:36a5:56a3] has joined ##hplusroadmap 01:53 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Remote host closed the connection] 02:38 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] 02:54 -!- augur [~augur@2601:640:8001:4222:35af:6628:36a5:56a3] has quit [Remote host closed the connection] 03:26 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 03:32 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap 03:38 -!- esmerelda [~mabel@2601:602:9603:a3f8:65e5:be41:3c9c:c816] has quit [Changing host] 03:38 -!- esmerelda [~mabel@unaffiliated/jacco] has joined ##hplusroadmap 03:58 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 03:58 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 240 seconds] 04:03 -!- Guest58277 is now known as abetusk 04:12 < kanzure> .title 04:12 < yoleaux> Erythritol is a pentose-phosphate pathway metabolite and associated with adiposity gain in young adults 04:24 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 04:45 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 260 seconds] 05:08 -!- jtimon [~quassel@117.29.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 05:37 < kanzure> .tw https://twitter.com/Addgene/status/864362031041847297 05:37 < yoleaux> New plasmids Nagai Lab: Genetically encoded ratiometric fluorescent thermometer @PLOSONE Note Find Plasmids link! http://hubs.ly/H07qTcf0 https://pbs.twimg.com/media/C_7Ur26VYAAROoK.jpg (@Addgene) 05:41 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 05:41 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 05:43 < kanzure> neural stem cell stuff: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2002329 https://twitter.com/PLOSBiology/status/864450371829346305 05:43 < kanzure> "Out of the resulting catalog of molecules predicted to affect SVZ microdomain-specific lineages, Azim et al. [16] prioritized compounds for further study by the number of target genes and gene ontology (GO) pathway analysis. This led them to zero in on 2 compounds as having particularly noteworthy effects: AR-A014418, which appears to rejuvenate the NSC lineage, and LY-294002, which promotes ... 05:43 < kanzure> ...development of oligodendrocytes by inhibiting PI3K/Akt signaling" ... "authors were able to promote regeneration in a mouse model of hypoxic brain injury, showing that GSK3? inhibitors allowed the recruitment of new oligodendrocytes and glutamatergic neurons into the cortex". 05:44 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 05:46 < kanzure> since we're unlikely to engineer proteins with internal state/memory any time soon, i was thinking about alternative methods of using error correction codes for error-prone dna synthesis 05:47 < kanzure> in particular perhaps there's a way of in vitro iterative application of recombinases/cas9/TALENs/something where after each round the dna molecule becomes increasingly less encoded and increasingly more matching the intended sequence 05:48 < kanzure> this is in contrast to a scheme where an enzyme would be responsible for reading the dna molecule and producing a translated decoded molecule all at once. 05:49 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 05:49 < kanzure> e.g. we would use a set of enzymes to convert from a molecule of encoded information to a molecule of ~1 bit of decoded information plus the remaining encoded information 05:52 < kanzure> it would also be interesting to investigate whether a ribosome could be forced to have polymerase activity-- where mRNA encodes nucleotide choice instead of amino acid choice. and you could have special nop nucleotide types between each codon. 05:53 -!- sachy [~sachy@nat.brmlab.cz] has joined ##hplusroadmap 05:54 < kanzure> insert like 20 nops between each codon- should fix frameshifting errors i think. 06:01 < kanzure> why aren't there any polymerases that check both strands of dna when they are making a new strand. isn't the whole point something about information redundancy and information theoretical preservation? blah. 06:02 < kanzure> i guess that wouldn't help-- in case of errors, which one would the enzyme pick? you'd need three strands, not two. 06:06 < kanzure> .tell yashgaroth we need a library of enzyme parts for basic dna string manipulation operations (addition, deletion, etc) with pluggable protein domains for targeting... e.g. stuff like "every [GTC] pattern, insert this other sequence". this sort of toolbox would be very helpful to me. 06:06 < yoleaux> kanzure: I'll pass your message to yashgaroth. 06:10 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 06:32 < kanzure> also, a way to have internal state inside of a protein, whether through conformational changes or otherwise, would be really nice. but that's just xmas wishlist stuff. 06:54 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 240 seconds] 07:04 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 07:06 -!- g0d355__ [~lmao@104.131.75.159] has joined ##hplusroadmap 07:12 -!- emeraldgreen [~user@3.ip-79-137-36.eu] has joined ##hplusroadmap 07:22 -!- emeraldgreen [~user@3.ip-79-137-36.eu] has quit [Quit: Leaving.] 07:26 < kanzure> "When has actual rejuvenation taken place? On a few occasions, 1) rejuvenation in cells, tissues and organs, caused by heterochronic transplantation, 2) rejuvenation of stem cells, tissues and organs using heterochronic parabiosis, cellular rejuvenation in mixed culture of young and old cells, 3) rejuvenation due to resetting cellular programming with embryonic transcription factors, the Yaman... 07:26 < kanzure> ...aka factors, being shown to produce rejuvenation in cells tissues and organs, and the use of young blood or young blood plasma to affect rejuvenation - a la Conboys's more recent efforts." 07:26 < kanzure> "If the theories of damage were true, then rejuvenation would be impossible, missing information cannot be made up for unless its provided exogenously, and that is not the case in actual rejuvenation." 07:28 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 07:53 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 246 seconds] 08:05 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Quit: Leaving.] 08:23 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 08:52 -!- augur [~augur@2601:640:8001:4222:35af:6628:36a5:56a3] has joined ##hplusroadmap 08:57 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 09:20 -!- augur [~augur@2601:640:8001:4222:35af:6628:36a5:56a3] has quit [Remote host closed the connection] 09:26 -!- g0d355__ [~lmao@104.131.75.159] has quit [Max SendQ exceeded] 09:45 -!- drewbot [~cinch@54.227.84.33] has quit [Remote host closed the connection] 10:12 -!- red-005 [red@gateway/shell/elitebnc/x-bfecicepamoiuzhj] has left ##hplusroadmap ["Leaving"] 10:31 -!- jcluck [~cluckj@pool-108-52-166-30.phlapa.fios.verizon.net] has joined ##hplusroadmap 10:34 -!- cluckj [~cluckj@pool-108-52-166-30.phlapa.fios.verizon.net] has quit [Ping timeout: 246 seconds] 10:56 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 10:57 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 11:05 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 11:09 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 11:10 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 11:11 -!- preview_ [~preview@2407:7000:842d:4000::3] has quit [Ping timeout: 246 seconds] 11:19 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Quit: Leaving] 11:19 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 11:24 -!- cluckj [~cluckj@pool-108-52-166-30.phlapa.fios.verizon.net] has joined ##hplusroadmap 11:25 -!- cluckj [~cluckj@pool-108-52-166-30.phlapa.fios.verizon.net] has quit [Client Quit] 11:34 -!- preview [~preview@210.246.62.101] has joined ##hplusroadmap 11:38 -!- jcluck is now known as cluckj 12:28 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 12:32 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 240 seconds] 12:42 < kanzure> the problem with in vitro stepwise decoding of a DNA molecule with cas9/recombinase/TALENs etc is that the error rate (or merely the yield) of the enzymatic steps might be higher (or for yield, lower) than the error rate of chemical DNA synthesis in the first place. 12:45 < kanzure> and why are all these enzymes mostly targeting palindromes.. they should be engineered to find specific unnatural nucleotides, instead. and then just chemically synthesize a DNA molecule with that alternative nucleotide. and then your edit sites can be based on addresses that include the unnatural nucleotide in a specific and known sequence. 12:51 < kanzure> "A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175349/ 12:54 < kanzure> actually i think zinc finger nucleases would probably work better for targeting... so engineer them to only recognize an unnatural nucleotide or something. or a sequence of them. or to only work after a series of nops. 12:55 < kanzure> "A promising new method to select novel zinc-finger arrays utilizes a bacterial two-hybrid system and has been dubbed "OPEN" by its creators.[2] This system combines pre-selected pools of individual zinc fingers that were each selected to bind a given triplet and then utilizes a second round of selection to obtain 3-finger arrays capable of binding a desired 9-bp sequence. This system was deve... 12:55 < kanzure> ...loped by the Zinc-Finger Consortium as an alternative to commercial sources of engineered zinc-finger arrays. See https://en.wikipedia.org/wiki/Zinc_finger_chimera for more information on zinc finger selection techniques.)" 12:55 < kanzure> .wik zinc finger chimera 12:55 < yoleaux> "Zinc finger protein chimera are chimeric proteins composed of a DNA-binding zinc finger protein domain and another domain through which the protein exerts its effect. The effector domain may be a transcriptional activator (A) or repressor (R), a methylation domain (M) or a nuclease (N)." -- https://en.wikipedia.org/wiki/Zinc_finger_chimera 12:56 < kanzure> "Linking together six ZFPs produces a target-site of 18-19 bp. Assuming specificity to that one sequence and that the sequence of the genome is random, 18 bp is long enough to be unique in all known genomes[3][4] " 13:02 < kanzure> .wik NgAgo 13:02 < yoleaux> "NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5? phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported to be suitable for ..." -- https://en.wikipedia.org/wiki/NgAgo 13:03 < kanzure> "Like the CRISPR/Cas system, NgAgo was reported to be suitable for genome editing,[1] but this has not been replicated. In contrast to Cas9, the NgAgo-gDNA system does not require a protospacer adjacent motif (PAM)" 13:03 -!- preview [~preview@210.246.62.101] has quit [Ping timeout: 240 seconds] 13:10 -!- emeraldgreen [~user@3.ip-79-137-36.eu] has joined ##hplusroadmap 13:19 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 13:21 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 272 seconds] 14:01 < nmz787_> kanzure: can you bid on that FIB? I am not home, and don't remember my password 14:01 < nmz787_> I guess worst case, I can reset it 14:03 < nmz787_> nevermind, they have some new temp password thing 14:06 -!- preview [~preview@103.23.18.14] has joined ##hplusroadmap 14:19 -!- preview [~preview@103.23.18.14] has quit [Remote host closed the connection] 14:21 -!- preview [~preview@103.23.18.14] has joined ##hplusroadmap 14:21 -!- preview [~preview@103.23.18.14] has quit [Remote host closed the connection] 14:22 -!- preview [~preview@103.23.18.14] has joined ##hplusroadmap 14:25 -!- preview [~preview@103.23.18.14] has quit [Remote host closed the connection] 14:25 -!- preview [~preview@103.23.18.14] has joined ##hplusroadmap 14:26 -!- preview [~preview@103.23.18.14] has quit [Remote host closed the connection] 14:27 -!- preview [~preview@103.23.18.14] has joined ##hplusroadmap 14:28 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 14:37 -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] 14:54 -!- SuperJen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 14:57 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 15:00 -!- poppingtonic [~brian@105.57.36.132] has joined ##hplusroadmap 15:00 -!- poppingtonic [~brian@105.57.36.132] has quit [Changing host] 15:00 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 15:05 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 260 seconds] 15:05 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 15:12 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 15:14 -!- SuperJen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 15:18 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 15:21 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 15:33 < kanzure> "Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase" https://academic.oup.com/nar/article/39/17/7868/2411376/Targeted-plasmid-integration-into-the-human-genome 15:33 < kanzure> above they do zinc-finger recombinase fusion proteins, v. cool 15:36 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Quit: Leaving.] 15:36 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Ping timeout: 240 seconds] 15:39 < kanzure> book chapter about zinc-finger recombinases http://www.scripps.edu/barbas/pdf/GajMethEnzymology2014.pdf 15:54 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=4f591ede Bryan Bishop: zinc-finger recombinases, yo >> http://diyhpl.us/diyhpluswiki/gene-editing/ 16:02 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 16:03 < kanzure> "A single zinc finger contains ~30 amino acids and typically functions by binding three consecutive base pairs of DNA via interactions of a single amino acid side chain per base pair ( 30 ). The specificity of particular zinc fingers for the 64 possible nucleotide triplets has been examined extensively through site-directed mutagenesis, rational design and the selection of large combinatoria... 16:03 < kanzure> ...l libraries ( 31-33 ). The modular structure of the zinc-finger motif permits the fusion of several domains in series, allowing for the recognition and targeting of extended sequences in multiples of 3 nucleotides ( 34 ). It is now possible to design synthetic zinc-finger proteins to bind practically any target site in the human genome ( 35 , 36 )" 16:03 < kanzure> v. cool. 16:07 < kanzure> "Inspired by the success of the ZFN technology, we have recently developed zinc-finger recombinases (ZFRs) to autonomously perform precise gene addition to the human genome without cleaving genomic DNA and activating the DNA damage response pathway ( 40 ). ZFRs are a fusion of a synthetic zinc-finger protein and the catalytic domain of a serine recombinase ( 41 , 42 ). For the chromosomal inte... 16:07 < kanzure> ...gration of plasmid DNA, the designed zinc-finger domain binds to specific target sites in the genome and the plasmid, and the recombinase domain catalyzes the exchange of DNA strands ( 40 )." 16:09 < kanzure> "Several other studies have investigated targeted gene addition by fusing engineered zinc-finger proteins or other targeted DNA-binding proteins to transposases ( 14 , 15 ) and retroviral integrases ( 54 , 55 ). Although these strategies have been successful in directing integration and transposition, the vast majority of integration events occur at locations other than the target locus. Impor... 16:09 < kanzure> ...tantly, these fusion proteins were not engineered to abrogate the integration activity of the parent enzyme. Consequently, any rare targeted integration events occur amidst many more semi-random events. This approach to protein engineering is in contrast to our development of ZFRs, in which the serine recombinase catalytic domain is only active when fused to a DNA-binding protein ( 40 )." 16:14 < kanzure> why do these recombinases have a recombinase target site if they have zinc finger dna binding domains attached to them? wouldn't it be better to have a promiscuous recombinase that only catalyzes when the zinc finger finds match dna? 16:35 -!- justan0theruser is now known as justanotheruser 16:42 < emeraldgreen> kanzure interesting 16:44 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 16:52 < emeraldgreen> >It is now possible to design synthetic zinc-finger proteins to bind practically any target site in the human genome ( 35 , 36 )" 16:52 < emeraldgreen> So, to implement this one needs a (semi-)automatic protein design tool, and also lab facilities for protein expression/purification and also a capability to synthesize DNA 17:00 -!- emeraldgreen [~user@3.ip-79-137-36.eu] has quit [Ping timeout: 240 seconds] 17:17 -!- emeraldgreen [~user@ppp91-122-98-9.pppoe.avangarddsl.ru] has joined ##hplusroadmap 17:29 -!- emeraldgreen [~user@ppp91-122-98-9.pppoe.avangarddsl.ru] has left ##hplusroadmap [] 17:38 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 260 seconds] 17:58 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 17:59 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 18:18 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 246 seconds] 18:42 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 240 seconds] 18:51 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 268 seconds] 18:53 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 19:07 < fenn> does "rejuvenation" extend life span? 19:18 -!- maaku [~mark@173.234.25.100] has quit [Quit: Lost terminal] 19:20 < streety> I think it would be generally expected to extend lifespan but I think you could call a process rejuvenation even if it did not extend lifespan 19:21 -!- maaku [~mark@173.234.25.100] has joined ##hplusroadmap 19:41 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:f5e0:f867:a11d:8d52] has joined ##hplusroadmap 19:45 -!- strages [uid11297@gateway/web/irccloud.com/x-vhmryucgyewtsoha] has quit [Quit: Connection closed for inactivity] 20:14 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 272 seconds] 20:15 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 20:22 < yashgaroth> what part of GW501516 causing cancer do people just ignore 20:22 < yoleaux> 16 May 2017 13:06Z yashgaroth: we need a library of enzyme parts for basic dna string manipulation operations (addition, deletion, etc) with pluggable protein domains for targeting... e.g. stuff like "every [GTC] pattern, insert this other sequence". this sort of toolbox would be very helpful to me. 20:24 < yashgaroth> you can possibly get enough specificity with TALENs but the error rate will be immense 20:26 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 20:29 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 20:36 -!- esmerelda [~mabel@unaffiliated/jacco] has quit [Ping timeout: 240 seconds] 20:36 -!- UnknownRogue [~UnknownRo@82.221.102.41] has joined ##hplusroadmap 20:37 -!- UnknownRogueX [~UnknownRo@82.221.102.41] has joined ##hplusroadmap 20:37 -!- UnknownRogueX [~UnknownRo@82.221.102.41] has quit [Remote host closed the connection] 20:37 -!- UnknownRogue [~UnknownRo@82.221.102.41] has quit [Remote host closed the connection] 20:38 -!- UnknownRogue [~UnknownRo@82.221.102.41] has joined ##hplusroadmap 20:49 -!- esmerelda [~mabel@2601:602:9603:a3f8:626d:c7ff:fe5f:86c7] has joined ##hplusroadmap 20:59 -!- preview [~preview@103.23.18.14] has quit [Ping timeout: 260 seconds] 21:10 -!- UnknownRogue [~UnknownRo@82.221.102.41] has quit [Ping timeout: 240 seconds] 22:30 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] 22:38 -!- preview [~preview@2407:7000:842d:4000::2] has joined ##hplusroadmap 22:41 -!- esmerelda [~mabel@2601:602:9603:a3f8:626d:c7ff:fe5f:86c7] has quit [Changing host] 22:41 -!- esmerelda [~mabel@unaffiliated/jacco] has joined ##hplusroadmap 22:41 < fenn> .title https://www.youtube.com/watch?v=Ul0Gilv5wvY 22:41 < yoleaux> Phase-Functioned Neural Networks for Character Control - YouTube 23:08 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 23:39 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Quit: Leaving.] 23:39 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap --- Log closed Wed May 17 00:00:56 2017