--- Log opened Fri Jun 16 00:00:23 2017
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05:05 < streety> It's not until the 4th sentence that the two-photon microscopy wikipedia entry actually mentions that two photons are absorbed.
05:10 < kanzure> streety, have you used two-photon microscopy?
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06:07 < streety> Not for one of my own projects. Attended a training course on confocal, two-photon, super res etc a couple years ago though so got to do a little imaging with one
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09:37 < nmz787_i> sup to all the aerobes in the room
09:37 < nmz787_i> or maybe... to all the aerobes in the room -- sup?
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09:39 < nmz787_i> streety: cool!
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09:45 < chris_99> played with your FIB nmz787_i ?
09:50 < nmz787_i> not yet! went home to get married for last week+
09:51 < chris_99> oh nice, congrats!
09:51 < nmz787_i> I plan to get some more allen-head sockets today for my ratchet handle
09:51 < nmz787_i> to swap the oil on my SEM diff pump
09:51 < chris_99> ah cool
09:51 < chris_99> do they normally use oil for those kind of pumps?
09:52 < nmz787_i> FIB stuff is blocked by needing to build a small room, which is blocked by needing to move some things around the garage
09:52 < nmz787_i> yeah, it is based on oil
09:52 < nmz787_i> .wik diffusion pump
09:52 < yoleaux> "Diffusion pumps use a high speed jet of vapor to direct gas molecules in the pump throat down into the bottom of the pump and out the exhaust." -- https://en.wikipedia.org/wiki/Diffusion_pump
09:52 < nmz787_i> the FIB has a turbo pump instead
09:53 < nmz787_i> less oil can contaminate the sample chamber, but the tradeoff is the spinning elements induce vibrations
09:53 < chris_99> ah, they're the things with tonnes of vane wheel things?
09:53 < nmz787_i> yeah
10:01 < chris_99> random question, have you ever seen affordable peristaltic pumps that can do >1lpm?
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10:22 < nmz787_i> hmm, I seem to remember the cheap pumps mostly did higher flow rates, so I'd guess they were > 1 liter a minute
10:22 < nmz787_i> I remember because I was always interested in very small and precise flow rates
10:23 < chris_99> https://www.aliexpress.com/item/1100ml-min-12Vdc-Peristaltic-Pump-with-Transparent-Orange-Exchangeable-Pump-Head-and-FDA-approved-PharMed-BPT/32552056363.html that was the cheapest 1lpm one i found on ali i think
10:23 < chris_99> but the shipping is the killer
10:26 < nmz787_i> I think I saw a 3d printed one recently
10:26 < chris_99> cool
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10:29 < nmz787_i> yeah I see most on amazon or ebay (UK) are >a few mL/min and less than 1 L/min
10:29 < nmz787_i> maybe you can find a cheaper one and gang them in parallel?
10:29 < chris_99> mm heh
10:38 < nmz787_i> .wik rosetta design
10:38 < yoleaux> "Rosetta@home is a distributed computing project for protein structure prediction on the Berkeley Open Infrastructure for Network Computing (BOINC) platform, run by the Baker laboratory at the University of Washington." -- https://en.wikipedia.org/wiki/Rosetta@home
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12:02 < poppingtonic> heh.. marblestone works for Kernel now..
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16:23 < streety> 3D printing would be a decent option for a high flow rate peristaltic pump. The tubing is going to eventually wear out anyway so you'll want easy replacements
16:23 < streety> How much above 1 L/minute are you looking for and what would affordable be?
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16:30 < streety> NIH are planning a citizen science workshop on July 14th https://videocast.nih.gov/summary.asp?live=21711&bhcp=1
16:30 < nmz787_i> .title http://www.eetimes.com/document.asp?doc_id=1331834&
16:30 < yoleaux> Toyota Going Open-Source in '18 Camry | EE Times
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16:32 < nmz787_i> hmm, maybe time to figure out how to download/save those NIH videocasts
16:34 < streety> They are actually really good for that. Multiple links for different resolutions. For example, see https://videocast.nih.gov/summary.asp?Live=18600&bhcp=1
16:34 < streety> click more and scroll to the bottom
16:37 < streety> My concern would be I've heard talk of it being aimed at NIH. Sometimes there are NIH only events, would be unusual for something like this though. Usually limited to bureaucracy related events
16:38 < streety> looks like DC Genomics and DMV Biotech groups have associated events https://www.meetup.com/DC-Genomics/ https://www.meetup.com/Biotechnology/events/240819566/
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17:10 < kanzure> something about haber process and fertilization and nitrogen fixation https://www.amazon.com/dp/0307351793/
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17:22 < kanzure> genehacker got a phd today
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17:47 < nmz787_i> nice!
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18:08 < kanzure> life of a programmer https://www.usenix.org/system/files/1311_05-08_mickens.pdf
18:10 < kanzure> and that one on byzantine fault tolerance https://www.usenix.org/system/files/login-logout_1305_mickens.pdf
18:10 < kanzure> ""How can you make a reliable computer service?" the presenter will ask in an innocent voice before continuing, "It may be difficult if you can't trust anything and the entire concept of happiness is a lie designed by unseen overlords of endless deceptive power." The presenter never explicitly says that last part, but everybody understands what's happening."
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--- Log closed Sat Jun 17 00:00:24 2017