--- Log opened Thu Sep 21 00:00:24 2017 00:04 -!- darsie [~username@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 00:11 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap 00:11 < CaptHindsight> https://scienmag.com/scientists-create-worlds-first-molecular-robot-capable-of-building-molecules/ 00:11 < CaptHindsight> might be of interest later today when the Nature article comes out 00:13 < CaptHindsight> http://www.nature.com/nature/journal/v549/n7672/full/nature23677.html 00:19 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds] 00:34 < darsie> grey goo, yeah 00:35 < fenn> always with the pessimism 00:36 < fenn> can't we just celebrate an advance for being a good thing? 00:48 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 00:56 < fenn> well it's a very small robot 00:56 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 00:57 < fenn> the shaded drawings make it look much bigger than it really is 00:59 < fenn> i'm not sure i'd call it "programmable" so much as they can manipulate what state it is in with chemicals 01:08 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 01:15 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 01:18 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 240 seconds] 02:15 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-wpebhvlwahcqkpic] has joined ##hplusroadmap 02:21 -!- traumschule [~traumschu@185.104.184.179] has quit [Ping timeout: 240 seconds] 02:31 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Remote host closed the connection] 03:16 < kanzure> .title 03:16 < yoleaux> Stereodivergent synthesis with a programmable molecular machine : Nature : Nature Research 03:25 -!- RebelCoder [~Yuriy@95.143.115.254] has quit [Ping timeout: 240 seconds] 03:25 -!- RebelCoder [~Yuriy@95.143.115.254] has joined ##hplusroadmap 03:45 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 264 seconds] 04:31 -!- jaboja [~jaboja@jaboja.pl] has quit [Remote host closed the connection] 04:36 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 05:22 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 05:36 -!- PresidentTrump is now known as NotNSA 05:37 -!- NotNSA is now known as PresidentTrump 05:59 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-wpebhvlwahcqkpic] has quit [Quit: Connection closed for inactivity] 06:03 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 06:20 < kanzure> hmph 06:25 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 248 seconds] 06:51 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 248 seconds] 07:16 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 255 seconds] 07:34 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 07:35 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 07:38 -!- traumschule [~traumschu@185.104.184.179] has joined ##hplusroadmap 07:44 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 07:45 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 07:51 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 248 seconds] 07:51 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 07:54 < kanzure> "dr. connectohm" 08:06 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 264 seconds] 08:09 < kanzure> "Open Philanthropy Project awards Genspace and NC State University $700,000 to support biosafety and biosecurity practices for the DIYbio community. The grant includes $454,025 to Daniel Grushkin, Executive Director of Genspace, and $252,725 to Todd Kuiken, Ph.D., senior research scholar the Genetic Engineering and Society Center at NC State University. The project includes two parts: a ... 08:10 < kanzure> ...comprehensive tour of DIYbio labs followed by a biosafety fellowship that places early career biosafety officers in these labs to work for a year and develop biosafety procedures and biosecurity provisions in the DIY lab context. The goal is to improve safety and security standards in the participating labs and to establish biosafety and biosecurity experts with a specialization in DIY labs. ... 08:10 < kanzure> ...Researchers expect the fellowship to begin in September 2018." 08:10 < cluckj> wow, good doing todd and dan 08:10 < kanzure> mandatory kuiken-sponsored security offers :( 08:10 < kanzure> this is bad 08:10 < cluckj> err going 08:10 < kanzure> this only further perpetuates the narrative that community labs are somehow diybio 08:10 < kanzure> and also, todd kuiken has been raising red flags for years 08:11 < cluckj> flags about what? 08:11 < kanzure> i mean he's shady as shit-- look at his writings; he confuses even basic facts about what diybio is. 08:11 < cluckj> ah 08:12 < kanzure> one of those 'social researchers' 08:13 < cluckj> ;) 08:19 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 240 seconds] 08:21 < kanzure> https://jasmcole.com/2017/09/20/the-moon-blew-up-without-warning-and-for-no-apparent-reason/ 08:24 -!- augur [~augur@198-27-215-123.static.sonic.net] has joined ##hplusroadmap 08:24 < kanzure> .wik kessler syndrome 08:24 < yoleaux> "The Kessler syndrome (also called the Kessler effect, collisional cascading or ablation cascade), proposed by the NASA scientist Donald J." — https://en.wikipedia.org/wiki/Kessler_syndrome 08:40 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Ping timeout: 248 seconds] 08:45 -!- sachy [~sachy@nat.brmlab.cz] has joined ##hplusroadmap 08:56 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 246 seconds] 08:59 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 09:29 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 09:55 -!- fltrz [d5db905c@gateway/web/freenode/ip.213.219.144.92] has quit [Ping timeout: 260 seconds] 09:59 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 255 seconds] 10:01 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 10:04 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 240 seconds] 10:07 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 10:14 -!- augur [~augur@198-27-215-123.static.sonic.net] has joined ##hplusroadmap 10:19 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Ping timeout: 240 seconds] 10:19 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 252 seconds] 10:20 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 11:02 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 11:05 < kanzure> 'Society for the rescue of our elders: A private association seeking to reverse human senescence' errr isn't it too late for the already-dead? 11:05 < kanzure> and it's just dasatinib and quercetin? 11:06 < kanzure> "A year-long clinical trial using once-a-week doses of rapamycin aims to begin in early October, 2017 in Thousand Oaks, California." 11:17 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 11:25 -!- augur [~augur@198-27-215-123.static.sonic.net] has joined ##hplusroadmap 11:29 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Ping timeout: 248 seconds] 11:34 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 11:37 -!- NikopolSohru [~NSohru@37.48.65.48] has quit [Ping timeout: 240 seconds] 11:46 -!- delinquentme [~delinquen@2602:306:ceb7:990:cdf7:d527:d537:2e8c] has quit [Ping timeout: 255 seconds] 11:51 -!- augur [~augur@198-27-215-123.static.sonic.net] has joined ##hplusroadmap 11:51 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 246 seconds] 11:52 < mrdata_> kanzure, you have an already-dead elder you wish to rescue? 11:53 < kanzure> if you're already old then senolytics aren't going to do much 11:54 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 11:56 -!- delinquentme [~delinquen@c-73-71-127-234.hsd1.ca.comcast.net] has joined ##hplusroadmap 12:03 -!- delinquentme_ [~delinquen@173-132-48-142.pools.spcsdns.net] has joined ##hplusroadmap 12:05 -!- delinquentme [~delinquen@c-73-71-127-234.hsd1.ca.comcast.net] has quit [Ping timeout: 255 seconds] 12:13 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 12:16 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Remote host closed the connection] 12:28 -!- augur [~augur@198-27-215-123.static.sonic.net] has joined ##hplusroadmap 12:33 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Remote host closed the connection] 12:43 < nmz787> this was OK DDRIO Initialization Late 12:43 < nmz787> ugh 12:43 < nmz787> that stereodivergent paper 12:44 < kanzure> ooooo nmz787 is leaking sensitive AMD secrets 12:45 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 12:54 -!- HEx1 [~HEx@hexwab.plus.com] has quit [Remote host closed the connection] 12:55 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 12:56 -!- NikopolSohru [~NSohru@37.48.65.48] has joined ##hplusroadmap 12:56 < kanzure> academia.edu continues its super creepy spam email campaign: "500 people recently read a paper you are mentioned in" 12:56 < nmz787> lol 12:57 < nmz787> they must be quoting the diyhpl.us citations 12:57 < kanzure> i'm all for tracking who-knows-what, but this is an extreme level of surveillance. 12:57 < nmz787> ( I kid) 12:59 < sachy> kanzure: 500 is just statistics, 487 during last week (+5 now) is more worring 13:01 < kanzure> "just statistics" 13:04 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap 13:08 -!- NikopolSohru [~NSohru@37.48.65.48] has quit [Ping timeout: 240 seconds] 13:15 -!- HEx1 [~HEx@hexwab.plus.com] has quit [Ping timeout: 260 seconds] 13:27 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 13:30 < kanzure> tensorflow model visualization http://idl.cs.washington.edu/papers/tfgraph/ 13:31 -!- NikopolSohru [~NSohru@37.48.65.48] has joined ##hplusroadmap 13:36 -!- augur [~augur@192.195.83.130] has joined ##hplusroadmap 13:44 < kanzure> .tw https://twitter.com/stochastician/status/910307372983132160 13:44 < yoleaux> If we ran the anti-ML-hype conference called "AI'nt gonna happen: the truth about how far machine learning has to go" would anyone speak? (@stochastician) 13:58 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 14:05 -!- preview [~quassel@2407:7000:842d:4000::3] has joined ##hplusroadmap 14:16 -!- augur [~augur@192.195.83.130] has quit [Remote host closed the connection] 14:17 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 14:42 -!- traumschule [~traumschu@185.104.184.179] has quit [Ping timeout: 260 seconds] 14:46 -!- Qfwfq [Qfwfq@gateway/shell/suchznc/x-vnrsxafndccaxczg] has quit [Ping timeout: 252 seconds] 14:47 -!- vikraman [~vh4x0r@gentoo/developer/vikraman] has quit [Ping timeout: 252 seconds] 14:47 -!- streety [streety@2a01:7e00::f03c:91ff:feae:ded6] has quit [Ping timeout: 252 seconds] 14:47 -!- streety_ [streety@2a01:7e00::f03c:91ff:feae:ded6] has joined ##hplusroadmap 14:47 -!- streety_ is now known as streety 14:49 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 14:49 -!- delinquentme_ [~delinquen@173-132-48-142.pools.spcsdns.net] has quit [Ping timeout: 260 seconds] 14:51 -!- delinquentme_ [~delinquen@173-132-48-142.pools.spcsdns.net] has joined ##hplusroadmap 14:53 < kanzure> nmz787: other than nucleosome data density, were there any other edits to the calculations you gave? 14:55 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 15:03 < kanzure> random aside, but one of the numbers that bill efcavitch gave for the column-based dna synthesizer is that its development (including phosphoramidite chemistry) took 200 man-years. 15:05 -!- vikraman [~vh4x0r@gentoo/developer/vikraman] has joined ##hplusroadmap 15:10 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 15:13 -!- preview [~quassel@2407:7000:842d:4000::3] has quit [Ping timeout: 246 seconds] 15:15 -!- preview [~quassel@2407:7000:842d:4000::3] has joined ##hplusroadmap 15:33 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 15:34 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 15:38 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 15:45 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 258 seconds] 15:46 < kanzure> "Formation of sprays from conical liquid sheets" https://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/20000101665.pdf 15:48 -!- delinquentme_ [~delinquen@173-132-48-142.pools.spcsdns.net] has quit [Ping timeout: 240 seconds] 15:49 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 15:50 -!- c0rw1n_ [~c0rw1n@206.41-244-81.adsl-dyn.isp.belgacom.be] has quit [Ping timeout: 252 seconds] 15:51 -!- preview [~quassel@2407:7000:842d:4000::3] has quit [Read error: No route to host] 15:51 < kanzure> droplet dispensing https://www.google.com/patents/US20040203173 15:52 < kanzure> man this sounds like inkjet to me. 15:52 -!- preview [~quassel@2407:7000:842d:4000::3] has joined ##hplusroadmap 15:53 -!- delinquentme_ [~delinquen@173-132-48-142.pools.spcsdns.net] has joined ##hplusroadmap 15:58 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 16:02 < kanzure> "pulse-jet deposition" ok i guess pulsejet is a more general term that twist is using 16:02 < kanzure> "Since it is a non-contact technique, pulse jet deposition does not result in scratching or damaging the surface of the support on which the arrays are synthesized. Pulse-jet techniques have very high deposition rate, which facilitates rapid manufacture of arrays." 16:03 < kanzure> "One problem that occurs in the dispensing of multiple droplets from a droplet dispensing device comprising multiple nozzles is that pressure transients cause a de-priming of a fluid meniscus on the tip of the nozzle. As a result, a drop is not dispensed from the nozzle and an error in the deposition process occurs. The pressure transients often are due to line noise or vibration of the ... 16:03 < kanzure> ...droplet dispensing device itself, both of which can result in vibration of the contents of the reservoir. This vibration causes the fluid reagent in the reservoir to become agitated. A pressure pulse travels through the droplet dispensing device causing the fluid meniscus to be sucked back into the nozzle chamber or causing the fluid meniscus to burst. In the latter circumstance, the fluid ... 16:03 < kanzure> ...reagent released by the bursting coats the face of the nozzle. In both circumstances, a failure in the deposition process is realized." 16:05 < nmz787> kanzure: I did come more calculations with the nanopore (read) sizes to determine what it would take to get to 6GBps... and did the same for write, assuming something like 50nm square area req 16:05 < kanzure> if the total reaction time per step is >1 minute then it might make sense to use a screw-aligned system that you replace each round. 16:05 < nmz787> kanzure: found the zhirnow+church paper to have pretty good/similar numbers actually 16:06 < kanzure> do you mean the kosuri+church 2014 paper? 16:06 < nmz787> no http://www.nature.com/nmat/journal/v15/n4/full/nmat4594.html 16:06 < nmz787> .title 16:06 < yoleaux> Nucleic acid memory : Nature Materials : Nature Research 16:06 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/Large-scale%20de%20novo%20DNA%20synthesis:%20technologies%20and%20applications%20-%20Church%20-%202014.pdf 16:06 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 16:06 < nmz787> I think it has a supplement as well 16:07 < nmz787> it has a great digram showing water is worse than time for DNA storage/retention 16:08 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 16:09 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Remote host closed the connection] 16:09 < kanzure> nmz787: anything you would like to see in src.org's summary? i'm putting together a few paragraphs. 16:12 < nmz787> current nanopore read technology: handling system is crude, not yet commercialized solid-state nanopores 16:12 < nmz787> 450 nucleotides per second per nanopore. 512 per USB-stick sized device. 16:12 < nmz787> assuming each was 50nm square, to reach 6GBps would require: 16:12 < nmz787> (((6000000000 byte/s * 8 bits/byte) / (450 nt/s *2 bits/nt)) * (50nm*50nm)) then convert those sq nm to sq mm and get 16:12 < nmz787> which yields 0.133 mm^2 16:13 < nmz787> lack of VLSI in all but maybe the oxford nanopore stuff 16:13 < nmz787> (commercially at least) 16:16 -!- delinquentme_ [~delinquen@173-132-48-142.pools.spcsdns.net] has quit [Ping timeout: 252 seconds] 16:24 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 16:25 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 16:32 -!- Qfwfq [Qfwfq@gateway/shell/suchznc/x-nanaopluhhhhbsoy] has joined ##hplusroadmap 16:32 -!- Qfwfq [Qfwfq@gateway/shell/suchznc/x-nanaopluhhhhbsoy] has quit [Changing host] 16:32 -!- Qfwfq [Qfwfq@unaffiliated/washirving] has joined ##hplusroadmap 16:32 -!- Qfwfq [Qfwfq@unaffiliated/washirving] has quit [Changing host] 16:32 -!- Qfwfq [Qfwfq@gateway/shell/suchznc/x-nanaopluhhhhbsoy] has joined ##hplusroadmap 16:39 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 16:47 -!- delinquentme_ [~delinquen@108-235-112-153.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 16:50 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 264 seconds] 16:51 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 16:54 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 17:06 < kanzure> .tw https://twitter.com/ESYudkowsky/status/910562482657271808 17:06 < yoleaux> @stochastician I'd love to see money bets at 9-1 on what *can't possibly* be done in AI in 1, 2, 3, and 5 years. Last time I asked I got few predictions. (@ESYudkowsky, in reply to tw:910307372983132160) 17:06 < kanzure> .tw https://twitter.com/ESYudkowsky/status/910563044081647616 17:06 < yoleaux> @stochastician One audience member afterwards came up to me and said we couldn't possibly get mouse-equivalent intelligence in 2 years. MOUSE-EQUIVALENT! (@ESYudkowsky, in reply to tw:910562482657271808) 17:06 < kanzure> .tw https://twitter.com/kanzure/status/910968733669224449 17:06 < yoleaux> @ESYudkowsky @stochastician how many years ago was this (@kanzure, in reply to tw:910563044081647616) 17:15 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 17:22 -!- esmerelda [~mabel@unaffiliated/jacco] has quit [Ping timeout: 240 seconds] 17:25 -!- Aurelius [~cpopell@50.35.79.117] has joined ##hplusroadmap 17:29 < kanzure> https://www.redhat.com/en/about/press-releases/red-hat-announces-broad-expansion-open-source-patent-promise# 17:31 < juul> anyone coming to the biosummit? 17:33 < juul> nmz787: i bought a nanodrop 1000 from an auction and it's coming up as a usb device and the magnet is activating when i initiate a scan but then the software gives me this error: https://juul.io/error.png 17:33 < kanzure> which one is biosummit 17:34 < juul> nmz787: any idea how to troubleshoot? 17:34 < juul> http://biosummit.org/ 17:37 < cluckj> nope :( 17:38 < kanzure> i dunno if i can personally scale well enough to handle this many conferences 17:39 < cluckj> wish I could, though 17:39 -!- preview [~quassel@2407:7000:842d:4000::3] has quit [Ping timeout: 252 seconds] 17:39 < cluckj> haha 17:41 < kanzure> i can type at most 30-50k words/day so... work backwards from there i guess. plus travel time. 17:41 < kanzure> juul: see pm 17:41 < cluckj> whoa, that is a lot of folks going 17:41 < kanzure> this is about +50 pages/day of transcripts 17:41 < kanzure> nmz787: i've literally had zero laptop problems since i've returned home. why? 17:46 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 17:48 < nmz787> juul: no idea on that error... but I might try another USB port, and a different USB cable to start 17:48 < nmz787> juul: maybe check for a reset/calibration mode or something 17:48 < juul> nmz787: heh i didn't actually try a different cable, but you're right of course. 17:49 < juul> do you happen to have the software for talking to the actual OceanOptics unit? maybe that could give me a more informative error 17:49 < nmz787> hmm 17:49 < nmz787> I think I did copy it once from a school machine 17:50 < nmz787> it was just the nanodrop software 17:50 < juul> their website has it for download but it's a zip with a password. haven't checked if it's one of the crackable ones yet 17:50 < nmz787> pretty sure the software copy I have is from an nd-100 17:50 < juul> ah ok. i have the nanodrop software 17:50 < nmz787> nd-1000 17:50 < nmz787> idk if there could be some differences? 17:50 < nmz787> keys, etc? 17:51 < nmz787> "engineering mode" unlocked, etc (I don't know if there was an "engineering mode" just thinking) 17:52 < nmz787> kanzure: not sure... pretty weird, but in a good way 17:52 < juul> i expect the oceanoptics stuff doesn't have the nanodrop-specific features like talking to the nanodrop pcb, but i don't really know. just wanted to see if it did anything differently 17:52 < nmz787> kanzure: done any software updates? 17:52 < kanzure> no 17:53 < nmz787> my guess is the nanodrop pcb is just off a GPIO/aux port on the OceanOptics USB2000 17:53 < nmz787> so probably something configurable with their main software 17:53 < nmz787> the error you show indicates it was written in LabView 17:53 < nmz787> (the NI indicates National Instruments) 17:55 < juul> yeah. i haven't worked with labview for ~15 years now 17:56 < juul> maybe i'll call their support when i get back from biosummit and see what happens 17:56 < kanzure> juul: please record audio. i don't think they're streaming. no way for me to type. 17:57 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 17:57 < juul> kanzure: ok, i will see what i can do but there are multiple concurrent tracks so it might be hard 17:57 < nmz787> we gotta get an apple watch that we send to conferences 17:58 < juul> what's with the apple watch? 18:00 < juul> specially good microphone or something? 18:00 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:01 < cluckj> they sell cheap and adequate voice recorders at Staples :P 18:04 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 18:08 < nmz787> idk, someone recently said they came to deciding on it for some audio speech recording reasons 18:09 < cluckj> sounds like they wanted an excuse to buy an apple watch :) 18:11 -!- MrHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap 18:12 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Read error: Connection reset by peer] 18:13 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 18:14 -!- anachronick [~kvirc@a85-138-233-38.cpe.netcabo.pt] has quit [Read error: Connection reset by peer] 18:15 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Ping timeout: 264 seconds] 18:16 < nmz787> I think it had to do with Apple opening up it's API, and I do see something about "3D spatial audio" 18:18 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 18:19 < nmz787> https://developer.apple.com/documentation/scenekit/scnaudiosource 18:20 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:20 < nmz787> maybe that's for games or something, idk 18:21 < cluckj> hah 18:23 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 18:28 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:f5e0:f867:a11d:8d52] has joined ##hplusroadmap 18:28 < juul> good spatial audio recording in a small device would be pretty cool 18:29 < kanzure> yes they want 24/7 audio recording 18:29 < kanzure> for life reasons 18:30 < kanzure> nmz787: so do you think these guys are washing by spraying the whole surface with acetonitrile (etc), or do you think they deposit it at each location and then blow nitrogen? i'm not sure why they would claim wash is the most expensive step if they are only depositing enough for each spot. 18:31 < kanzure> sorry why did i say nitrogen. i mean argon. 18:32 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 18:45 < fenn> i wonder what percentage of the organizations people have listed on the biosummit page are real, vs how many are made up names for their personal garage hacking 18:46 < kanzure> would you be disappointed if they are all real names? 18:46 < fenn> no 18:46 < fenn> i find it hard to believe there are actually that many bio-hackerspaces 18:46 < kanzure> there's a lot of cities 18:48 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:49 < cluckj> there's a lot of biohacker spaces now 18:53 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Read error: Connection reset by peer] 18:53 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 18:56 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 18:57 < nmz787> kanzure: I've not heard of them using any sort of drain system... so I guess they are actually being just that sloppy 18:59 < kanzure> well it doesn't need a drain: you just blow it away with argon. but you don't need to nuke the *entire* surface with acetonitrile. 19:00 < kanzure> certainly in column synthesizers, sure, i can buy "wash is expensive" absolutely. 19:01 < fenn> there's definitely some region between "spray with a fog of acetonitrile" and "pour a stream of acetonitrile out of a faucet" 19:02 < fenn> i don't think inkjetting solvent will work 19:02 < kanzure> elaborate 19:02 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 19:03 < fenn> the drop of solvent would evaporate before you could move it very far 19:03 < fenn> and there wouldn't be enough solvent to saturate the atmosphere around the droplet 19:03 < kanzure> what about: blow stream of argon, jet some solvent to specific locations, agitate (whatever that means for this solvent- i dunno, maybe heat? who knows), then blow with more argon everywhere. 19:04 < fenn> maybe if you blew solvent saturated argon the droplet would last long enough to go somewhere 19:04 < kanzure> you're saying the solvent droplet evaporates during the travel time from dispensing to delivery at its destination spot? 19:05 < fenn> but i think that would use just as much solvent as a minimum amount of fog spray 19:05 < kanzure> AFAIK they aren't even using solvent fog sprays 19:05 -!- darsie [~username@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 240 seconds] 19:05 < fenn> the droplet evaporates somewhere on the surface, leaving a deposit of whatever it was carrying 19:05 < kanzure> hmm. 19:05 < kanzure> because of the argon? 19:06 < fenn> yes and because it's wetting the surface, leaving fragments of droplets aroudn 19:06 < fenn> but mostly because you're blowing on it and the local atmosphere is not saturated 19:06 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 19:06 < kanzure> hypochondriac super-powers, activate! 19:07 < fenn> leishmaniasis, it must be 19:07 -!- mrdata__ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 19:08 < kanzure> blowing argon isn't enough- you are forcing the garbage to stick to the surface or whatever, probably. 19:08 < kanzure> hydrophobic surfaces would help a little, right? 19:08 < kanzure> if you have wells that are not hydrophobic, then you end up with the material stuck inside of there after blowing.. hm. 19:09 < fenn> if you have wells then you have a more difficult droplet targeting problem 19:09 < fenn> or i dunno maybe an easier problem 19:09 < kanzure> i was thinking of wells that are really more like wide funnels 19:09 < kanzure> instead of holes with sharp edges 19:09 < kanzure> 'indentations' 19:10 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 246 seconds] 19:10 < fenn> or maybe bumps with hydrophilic tops 19:10 < kanzure> i wonder if you could balance a droplet on a hydrophilic 'tip' 19:11 < kanzure> realistically tho you still use a bead at that point, and the oligos are attached to the bead, and the bead has surface area that can attract dirt 19:12 < kanzure> all of the "physical" cleaning methods that i know about are really extreme like bake in oven at 8 million degrees or whatever. which is inappropriate if you want your oligos to survive. 19:13 -!- mrdata__ [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds] 19:13 < fenn> the wash step is just to remove the protecting group 19:13 < kanzure> there are multiple wash steps per cycle. 19:13 < kanzure> oxidate, wash, detriylate, wash, print, wash, etc. 19:13 < fenn> because each "cycle" is all four base pairs? 19:14 < nmz787> yeah but you need at least 3 good washes to get over le chatliers principle 19:14 < nmz787> you aren't just adding solvent 19:14 < nmz787> you are washing 19:14 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 19:14 < nmz787> just like you wash rain spots off your car 19:14 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 19:14 < fenn> i like this hydrophilic bumps on a hydrophobic surface idea 19:14 < fenn> it reduces the actual surface area that needs to be washed 19:15 < kanzure> in the nanochannel case you just make sure none of the reagents stick to the channel surfaces, right? and then you just keep flowing solvent over the thing? 19:17 < nmz787> in a nanochannel you'd probably want to do liquid-phase chemistry, where you'd precipitate the DNA, then rinse it, then re-suspend it 19:17 < nmz787> but even if you were doing solid-phase 19:17 < nmz787> you just need to pump 3X the volume of the polymerizing area through 19:17 < nmz787> pretty much 19:18 < nmz787> depends on how many 9s you want on your purity calculation 19:18 < nmz787> but 3X is my personal minimum for chemistry and i.e. washing dishes 19:18 < nmz787> .wik le chatelier's principle 19:18 < yoleaux> ", Le Chatelier's principle (pronounced /lə ˈʃɑːtəlieɪ/), also called Chatelier's principle or "The Equilibrium Law", can be used to predict the effect of a change in conditions on a chemical equilibrium. The principle is named after Henry Louis Le Chatelier and sometimes Karl Ferdinand Braun who discovered it independently. It can be stated as:" — https://en.wikipedia.org/wiki/Le_chatelier%27s_principle 19:18 < kanzure> i'm a little confused about the actual wash requirements. 19:18 < kanzure> so say we do fenn's idea 19:19 < kanzure> and you add solvent to a droplet and you maybe agitate it somehow to get it to circulate around, maybe add heat and scrubber c60 fullerenes or whatever, who knows, 19:19 < kanzure> then you need to physically remove the liquid for a bit 19:19 < nmz787> heat will just dry the solvent, concentrating the waste 19:19 < kanzure> s/for a bit/in preparation for the next step 19:20 < fenn> ultrasound 19:20 < kanzure> if you use argon jet, you are blowing the waste into the other spots on the surface 19:20 < nmz787> which is why they do mass washing 19:20 < kanzure> (evaporation of waste would be nice) 19:20 < nmz787> dunk the whole plate! 19:20 < fenn> i was assuming you couldn't just reuse the solvent 19:20 < nmz787> and they even mentioned some places do acetonitrile distilling, to recover 19:20 < kanzure> yeah why don't they dunk? 19:21 < nmz787> kanzure: they basically do 19:21 < fenn> yes distillation would be simpler 19:21 < kanzure> they don't dunk they re-splather the whole surface with new acetonitrile 19:21 < nmz787> yeah but I mean same amount of reagent relatively 19:21 < kanzure> fenn means have a liquid bath, flip the surface, dunk it, then dry 19:22 < nmz787> idk, seems the same as top-rinse 19:22 < kanzure> and liquid bath stays for some number of reactions 19:22 < nmz787> more mechanically complex 19:22 < fenn> i never said anything about a bath 19:22 < nmz787> lol 19:22 < kanzure> thought you were going to say a bath. ok. 19:23 < kanzure> anyway, with ultrasound you are also pushing contaminant back into the other spots, just like argon blower 19:23 < fenn> ultrasound is just to agitate the solvent 19:23 < nmz787> come to the plumbing side 19:23 < nmz787> indoor plumbing did wonders for society 19:23 < kanzure> surface plumbing? 19:24 < nmz787> only monkeys fling shit through the air 19:24 * fenn imitates nmz787 "imagine the glorious future, billions of tiny bathtubs on a chip!~" 19:24 < kanzure> fenn, what about 45 degree angle surfaces on the surface. so everythig is on a slant. and then everything drains downwards. 19:24 < nmz787> that's like indoor egg farming 19:24 < kanzure> and it's still hydrophilic, so it doesn't drain immeditely, but you can poke it (with argon or whatever) to move into the drain 19:24 < nmz787> I wanna eat the eggs from the top cages! 19:25 < fenn> surface tension is way stronger than gravity at those scales 19:25 < nmz787> kanzure: we really need VLSI examples 19:25 < kanzure> well with femotosecond laser blahblahblah you can construct the surfaces to move the water along a gradient due to hydrophobicity or whatever... but ok. 19:25 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] 19:26 < fenn> i was thinking the droplet of solvent would be perched on top of a hydrophilic bump, and then blown away by a jet of argon, taking all the junk with it 19:26 < fenn> if it evaporates mid-flight that doesn't matter 19:26 < kanzure> well i was thinking about where i've seen argon jet before, and it's essentially in the same cases as "flood the surface with acetonitrile", and it makes me wonder- isn't this contaminating other spots? 19:27 < fenn> if you need to turn it upside down to keep the stuff from landing again on the substrate, so be it 19:27 < kanzure> hm you can probably do holes on the surface and blow argon from underneath 19:27 < kanzure> (after flipping) 19:27 < fenn> it's not contaminating because the dna strand is covalently bound to the surface 19:28 < kanzure> (yes yes i meant garbage contamination only) 19:28 < fenn> ok maybe there will be tiny amounts of dNTP contamination, and that would raise the error rate 19:28 < kanzure> anyway if you flip the surface and force argon through the hockey table, the drops fall off and downward 19:28 < kanzure> (actually i don't know how hockey tables work so i shouldn't say that) 19:28 < nmz787> you mean air hockey 19:28 < fenn> i think the jet would be on the inkjet head along with the solvent sprayer 19:29 < nmz787> and those are drains 19:29 < kanzure> yeah air hockey, sorry 19:29 < nmz787> just apply vacuum 19:29 < fenn> the solvent sprayer is on the front and the jet is on the back, the distance gives the solvent some time to work 19:29 < nmz787> that doesn't sound right 19:29 < kanzure> pulse jet works so quickly that you will always be ahead of the solvent by multiple seconds... the argon sprayer can be anywhere. it doesn't matter. 19:30 < kanzure> *ahead of the solvent action 19:30 < kanzure> by the time you're done jetting the solvent on the surface, it still wont be time to start argon spray at even the first spot 19:31 < kanzure> ... unless the inkjet head, or the surface, is absolutely enormous :-) 19:31 < fenn> how long does it take? 19:31 < kanzure> well in general the whole cycle time for phosphoramidite chemistry is like 2 minutes 19:31 < fenn> these are really tiny pieces of garbage that are being dissolved 19:31 < kanzure> you can print a lot of droplets in 2 minutes 19:32 < fenn> what do you think about reel to reel substrate 19:32 < kanzure> not opposed 19:33 < nmz787> kanzure: you are working backwards from 1TB/day write speed? 19:33 < fenn> i am imagining a giant 8-track tape that just constantly prints and washes 19:33 < nmz787> industry had me work backwards from 6GB/s 19:33 < kanzure> nmz787: nope. i haven't considered write throughput (or speed) at all. i am working backwards from like.. inkjet printhead scaling limits. 19:33 < fenn> even better if it's a moebius strip because then you can use both sides of the tape 19:34 < nmz787> kanzure: but then this might all be in vain if it can't hit the customer's minimum (first-gen) requirement 19:34 < kanzure> yeah an industrial scale inkjet dna synthesizer should be operating constantly and have basically infinite surface area if you consider surfaces to be reusable at some rate 19:34 < kanzure> nmz787: the more magic methods still require more research. i am taking an engineering approach to this. 19:35 < kanzure> nmz787: besides, they aren't my customer. they want to fund twist- that's what the meeting was about. 19:35 < fenn> by "giant" i mean the width of the tape is the width of one inkjet head, and the length is the amount of area that can be printed in one cycle time 19:36 < kanzure> length constrained to printable area of one cycle. hrm. 19:36 < fenn> it can be longer but not shorter 19:36 < fenn> if it's shorter you have to stop, and that's wasting inkjet head time 19:36 < nmz787> kanzure: but engineering approach would first start with, can your approach meet the requirements... if you need a warehouse of inkjets, that might not be a solution for a warehouse of HDDs 19:36 < kanzure> "warehouse of inkjets" is inappropriate; you should just build an inkjet the size of a warehouse instead. this is how scaling works. 19:37 < nmz787> none of this fits into your prior "planar everything" approach... you were pitching combustion engines! 19:37 < fenn> i'd rather have a warehouse of inkjets 19:37 < fenn> easier to replace broken parts if it's modular 19:37 < fenn> see: "cube" by borg et al. 19:37 < kanzure> fenn: well the question here is total throughput 19:37 < fenn> how long is a rope 19:37 < kanzure> total throughput of super large inkjet printhead > total throughput of many inkjets 19:38 < fenn> that's dumb, it should be throughput per dollar 19:38 < nmz787> ^ 19:38 < fenn> amortized over the time until the singularity 19:38 < kanzure> well "per dollar" is like... some sort of calculation of what you can deliver over all the possibilities of engineering-dollars spent. 19:38 < nmz787> you aren't gonna be playing FLAC off your DNA miniDrive with inkjet tech 19:39 < kanzure> i never promised anyone flac audio 19:39 < fenn> dna mixtape 19:39 < nmz787> yo yo yo 19:39 < fenn> yo yo yo dawg i herd u like splicing so i put a splicer in your splicer 19:40 < nmz787> that's some transposon shit right there! 19:40 < kanzure> anyway why would you have to wash if your nanochannel is hydrophobic and you're always flowing liquid by the oligo? 19:41 < nmz787> https://www.youtube.com/watch?v=PwHNBxqVzA0 19:41 < nmz787> .title 19:41 < yoleaux> Quantum Shit - YouTube 19:42 < nmz787> kanzure: you wouldn't be always flowing 19:42 < nmz787> kanzure: you need some time to react, for a given temperature 19:42 < kanzure> so? you flow some neutral-ish liquid later. 19:42 < nmz787> yeah, whatever is compatible with rinsing the stuff you want to wash away 19:43 < kanzure> ok maybe their claim about "wash is so expensive" was not re: microfluidic schemes 19:43 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 19:45 < kanzure> "directed greybox fuzzing with AFL" https://github.com/aflgo/aflgo 19:46 < kanzure> http://lcamtuf.coredump.cx/afl/ 19:47 -!- PresidentTrump [~President@unaffiliated/presidenttrump] has quit [Remote host closed the connection] 19:48 < kanzure> i'm still upset about their comment about "a billion valves is not competitive". why/how. 19:48 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 19:49 < kanzure> it was sort of a throw-away comment in the heat of the moment so there wasn't time to challenge it 19:49 < kanzure> who gave that comment? it was someone about four to nine people over to my right hand side. 19:52 < kanzure> it was either the dodoomnidata person, bramlett, fahim, or magyar. the other people don't make sense to give that comment. maybe a church lab person? it wasn't kettner. 19:52 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 19:52 < kanzure> blargh 19:52 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 19:52 < nmz787> I thought maybe the Twist guy 19:53 < nmz787> Bill 19:54 < kanzure> oh that might be right. it did sound like him. 19:54 < kanzure> no i mean the other bill 19:54 < kanzure> bill was on the other side of the room 19:54 < kanzure> twist can't say that's not competitive.. how would that be true. what. 19:58 < kanzure> "A billion nozzles in a row is not competitive with current production rates" okay well that was in a row.... 20:00 < kanzure> if that was true then it would mean someone is synthesizing more than 1 billion spots per cycle ?? 20:00 < kanzure> maybe they assumed the surface doesn't change..? 20:08 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Read error: Connection reset by peer] 20:09 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 20:12 < nmz787> https://www.linkedin.com/pulse/28gbps-microstrip-pepper-jack-cheese-substrate-ryan-lott 20:28 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 20:32 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Ping timeout: 240 seconds] 20:37 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 20:51 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 21:10 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 21:10 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 21:17 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 248 seconds] 21:23 -!- jaboja [~jaboja@jaboja.pl] has quit [Remote host closed the connection] 21:42 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 21:51 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 22:09 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 22:12 -!- MrHindsight [~2020@unaffiliated/capthindsight] has quit [Ping timeout: 240 seconds] 22:21 -!- TC [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 22:21 -!- TC is now known as Guest57324 22:25 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 240 seconds] 22:38 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] 23:00 < fenn> monterey jack is a superior substrate, no yucky peppers to interfere with its integrity 23:01 < fenn> also when did linkedin become a blog platform 23:37 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 264 seconds] 23:43 -!- aeiousomething [~aeiousome@223.237.22.121] has joined ##hplusroadmap 23:44 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 23:47 -!- aeiousomething [~aeiousome@223.237.22.121] has quit [Ping timeout: 252 seconds] --- Log closed Fri Sep 22 00:00:25 2017