--- Log opened Sat Sep 23 00:00:11 2017 --- Day changed Sat Sep 23 2017 00:00 -!- delinquentme [~delinquen@108-235-112-153.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 00:00 -!- delinquentme [~delinquen@108-235-112-153.lightspeed.sntcca.sbcglobal.net] has quit [Client Quit] 00:15 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 01:05 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-sovpvugoezxwimfy] has joined ##hplusroadmap 02:26 -!- NikopolSohru [~NSohru@s5596eed8.adsl.online.nl] has joined ##hplusroadmap 02:41 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 02:43 -!- traumschule [~traumschu@185.104.184.179] has joined ##hplusroadmap 03:19 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-sovpvugoezxwimfy] has quit [Quit: Connection closed for inactivity] 03:43 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-lvgdhonmxihcybrl] has joined ##hplusroadmap 04:05 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 240 seconds] 04:07 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 04:15 -!- darsie [~username@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 04:21 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 04:24 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds] 04:40 -!- mrdata__ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 04:43 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds] 04:43 < kanzure> hmph 05:00 < kanzure> .tw https://twitter.com/biobricks/status/911309157025771521 05:00 < yoleaux> BBF is pleased to support #globalbiosummit and encourages requests from biohackers for 10k genes to be shared via #bionet and #openMTA (@biobricks) 05:02 < kanzure> https://synbiobeta.com/future-trades-genes-dna-synthesis-shaping-new-bio-economy/ 05:03 < kanzure> http://www.synthesis.cc/synthesis/2015/05/brewing_bad_biosecurity_policy 05:04 < kanzure> "That is, we have multiple, clear demonstrations that, rather than improving the world, restricting access to distributed production can instead cause harm. But, really, when has this ever worked? And why do people think going down the same path in the future will lead anywhere else? I am still looking for data – any data at all – that supports the assertion that regulating biological ... 05:04 < kanzure> ...technologies will have any different result." 05:14 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 05:30 -!- traumschule [~traumschu@185.104.184.179] has quit [Ping timeout: 240 seconds] 05:33 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 240 seconds] 05:35 -!- traumschule [~traumschu@185.104.184.179] has joined ##hplusroadmap 05:36 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 05:55 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 240 seconds] 06:01 < cluckj> any livestream links for this morning? 06:02 < cluckj> answered my own question: https://www.youtube.com/watch?v=Tl-0_XeGjNI 06:02 -!- preview [~quassel@2407:7000:842d:4000::3] has quit [Ping timeout: 240 seconds] 06:08 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 06:14 < kanzure> .title 06:14 < yoleaux> Bio Summit Live Stream - YouTube 06:28 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 06:45 -!- mrdata__ is now known as mrdata 06:52 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 240 seconds] 07:04 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 260 seconds] 07:07 -!- thxffo [SMD@gateway/shell/elitebnc/x-xwfccpdmecmjmrtb] has quit [Ping timeout: 276 seconds] 07:13 -!- thxffo [SMD@gateway/shell/elitebnc/x-pxftiecwzmrojvcv] has joined ##hplusroadmap 07:27 -!- querty [~jon@cpc69049-oxfd25-2-0-cust945.4-3.cable.virginm.net] has joined ##hplusroadmap 07:36 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 07:36 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 07:38 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 255 seconds] 07:39 -!- mrdata__ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 07:42 < kanzure> https://techcrunch.com/2017/09/22/crispr-breakthrough-could-drop-miscarriage-rates/ 07:42 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-lvgdhonmxihcybrl] has quit [Quit: Connection closed for inactivity] 07:42 < kanzure> "Genome editing reveals a role for OCT4 in human embryogenesis" https://www.nature.com/nature/journal/vaop/ncurrent/full/nature24033.html 07:42 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds] 07:46 -!- mindsForge [~nak@174-26-65-3.phnx.qwest.net] has joined ##hplusroadmap 07:53 -!- mrdata__ [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds] 07:54 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 08:17 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 08:18 < JayDugger> Good morning. 08:19 -!- querty [~jon@cpc69049-oxfd25-2-0-cust945.4-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 08:21 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 260 seconds] 08:21 -!- querty [~jon@cpc69049-oxfd25-2-0-cust945.4-3.cable.virginm.net] has joined ##hplusroadmap 08:50 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 08:59 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 09:28 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 09:48 -!- fltrz [4d6d6154@gateway/web/freenode/ip.77.109.97.84] has joined ##hplusroadmap 09:51 < kanzure> fltrz: what's up 09:51 < fltrz> hey, the usual I guess 09:51 < fltrz> inspecting this second hand CRT doorbell thing 09:52 < fltrz> trying to figure out what the easiest way would be to disable audio (already done one way), disable the timeouts that shut the camera and monitor off, perhaps remove the dependency on the camera so it just displays a blank screen... 09:53 < fltrz> for the entangled photon experiment 09:56 < fltrz> the doorbell was a stupid idea, old b/w tv's at least have analog inputs so I wouldn't need to mess around like this 09:56 < fltrz> I remembered them as brighter, but really I probably just misremembered how much they flickered 09:57 < fltrz> kanzure: what you up to? 09:58 < kanzure> i think i was going to go make a list of polymerase active residues 09:58 < kanzure> something something http://diyhpl.us/wiki/gene-editing/ 10:01 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=e4550750 Bryan Bishop: whoops forgot aobut reverse transcriptases >> http://diyhpl.us/diyhpluswiki/gene-editing/ 10:02 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 10:04 < kanzure> music https://www.youtube.com/watch?v=mHaSgZqC21Q 10:44 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 11:03 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 11:28 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 264 seconds] 11:32 -!- mindsForge [~nak@174-26-65-3.phnx.qwest.net] has quit [Remote host closed the connection] 11:35 < kanzure> "Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication" ... 11:35 < kanzure> ...https://www.researchgate.net/profile/Andreas_Broedel/publication/319051754_Intracellular_directed_evolution_of_proteins_from_combinatorial_libraries_based_on_conditional_phage_replication/links/598df5ff458515b87b25542d/Intracellular-directed-evolution-of-proteins-from-combinatorial-libraries-based-on-conditional-phage-replication.pdf 11:36 < kanzure> "Extending enzyme molecular recognition with an expanded amino acid alphabet" http://www.pnas.org/content/114/10/2610.abstract 11:41 < kanzure> nanowire internalization into cells by using a coating of peptides https://cml.harvard.edu/assets/NanoLett_2016_16_1509-1513_Lee.pdf 11:44 < kanzure> radio/sdr things http://sdr.hu/ http://websdr.ewi.utwente.nl:8901/ http://websdr.org/ 11:47 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 11:48 -!- querty [~jon@cpc69049-oxfd25-2-0-cust945.4-3.cable.virginm.net] has quit [Remote host closed the connection] 11:49 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 264 seconds] 12:24 < nmz787> fltrz: can't you just use an old TV? around here I'd expect to find one from $5 to $20... though it might take driving around to a few stores for an hour or two 12:24 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 246 seconds] 12:29 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-yieikzhyrusfirlr] has joined ##hplusroadmap 12:57 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 13:27 -!- strages [uid11297@gateway/web/irccloud.com/x-usddnuvuyeicgknz] has quit [Quit: Connection closed for inactivity] 13:29 -!- eb3c90|2 [~kvirc@host109-155-47-255.range109-155.btcentralplus.com] has joined ##hplusroadmap 14:00 < fltrz> nmz787: yes, I can see 15 euro b/w mini TVs, I plan to get one some day 14:18 -!- mindsForge [~nak@174-26-65-3.phnx.qwest.net] has joined ##hplusroadmap 14:19 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 14:24 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 14:33 < fltrz> I just tried the entangled photon experiment, but could not see the effect with the naked eye,.. the stimulation effect was visible though.. 14:33 < fltrz> perhaps its still measurable 14:34 < fltrz> (the original authors never claimed the effect to be visible with the naked eye) 14:41 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 14:46 < mindsForge> an entangled photon experiment in a home lab? 14:47 < mindsForge> fltrz: any esoteric/expensive supplies you have to get? 14:48 < mindsForge> I have to admit I didn't delve too deep into the logs if this was already covered :P 14:51 < fltrz> mindsForge: no, its not expensive because its not at single photon levels 14:52 < fltrz> mindsForge: just trying to reproduce https://arxiv.org/pdf/physics/0503052.pdf 14:52 < fltrz> for now I have only reproduced the stimulated emission of the phosphors 14:53 < fltrz> now I need suitable entangled photon source 14:54 < fltrz> perhaps the crt suffices, but I just need to program either my FPGA & ADC or an Arduino to log the light level over time 14:55 < fltrz> I will probably get suitable mercury lamp, b/w mini TV, reverse SHG from lasers etc to try different entangled photon sources 14:56 < fltrz> but I guess I will postpone it till after my next working period 14:59 -!- delinquentme [~delinquen@2602:306:ceb7:990:5c9e:4fcb:45e3:7a2] has joined ##hplusroadmap 14:59 < mindsForge> interesting, I'll check it out, what kind of sensors are you looking into for logging light levels? 15:00 < fltrz> I am just using standard photo diode for the moment, nothing fancy, BPW34 15:00 < fltrz> in reverse biased photoconductive mode with opamp 15:01 < fltrz> textbook photodiode TIA 15:01 < mindsForge> nice 15:01 < fltrz> costs nothing 15:01 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 246 seconds] 15:03 < fltrz> phosphors are about 5 different glow-in-the-dark materials from art supply store (2 bottles of paint, 2 sheets of paper coated with phosphorescent material, something something) most were ZnS according to datasheet, one was another, and others didn't say in MSDS etc 15:04 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 15:04 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Client Quit] 15:05 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 15:05 -!- traumschule [~traumschu@185.104.184.179] has quit [Ping timeout: 260 seconds] 15:06 -!- strages [uid11297@gateway/web/irccloud.com/x-kbwzwgugfagagptx] has joined ##hplusroadmap 15:25 -!- preview [~quassel@2407:7000:842d:4000::3] has joined ##hplusroadmap 15:29 -!- delinquentme [~delinquen@2602:306:ceb7:990:5c9e:4fcb:45e3:7a2] has quit [Ping timeout: 264 seconds] 16:07 -!- delinquentme [~delinquen@96-68-162-179-static.hfc.comcastbusiness.net] has joined ##hplusroadmap 16:40 -!- delinquentme [~delinquen@96-68-162-179-static.hfc.comcastbusiness.net] has quit [Quit: Leaving] 16:41 -!- delinquentme [~delinquen@96-68-162-179-static.hfc.comcastbusiness.net] has joined ##hplusroadmap 17:09 < delinquentme> kanzure, whos the local CNT guru here? 17:22 -!- eb3c90|2 [~kvirc@host109-155-47-255.range109-155.btcentralplus.com] has quit [Ping timeout: 252 seconds] 17:47 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] 17:48 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-yieikzhyrusfirlr] has quit [Quit: Connection closed for inactivity] 17:52 -!- delinquentme [~delinquen@96-68-162-179-static.hfc.comcastbusiness.net] has quit [Quit: Leaving] 17:53 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 17:59 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 18:13 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 18:17 < kanzure> uhh i guess genehacker 18:22 -!- delinquentme [~delinquen@96-68-162-179-static.hfc.comcastbusiness.net] has joined ##hplusroadmap 18:36 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Read error: Connection reset by peer] 18:37 < kanzure> .tw https://twitter.com/Strange_Animals/status/911662725868032000 18:37 < yoleaux> The stunning jewel caterpillar. https://pbs.twimg.com/media/DKbgatEVoAAJb5x.jpg (@Strange_Animals) 18:37 < kanzure> looks delicious 18:44 < kanzure> alright get cracking http://igem.org/Team_Wikis?year=2017 18:44 < kanzure> gotta write summaries http://diyhpl.us/wiki/dna/projects/ 18:47 < kanzure> 'Fusing ‬a ‬DNA-binding TAL ‬domain ‬‬to ‬a ‬histone ‬modifying ‬enzyme, such as ‬a ‬demethylase, ‬has ‬been ‬proven ‬to ‬influence ‬gene ‬expression' 19:02 < kanzure> music https://www.youtube.com/watch?v=-9pgIVcB3rk 19:07 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 19:15 -!- strages [uid11297@gateway/web/irccloud.com/x-kbwzwgugfagagptx] has quit [Quit: Connection closed for inactivity] 19:16 -!- jaboja [~jaboja@jaboja.pl] has quit [Remote host closed the connection] 19:26 -!- delinquentme [~delinquen@96-68-162-179-static.hfc.comcastbusiness.net] has quit [Ping timeout: 248 seconds] 19:41 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 19:46 < kanzure> blort 19:47 -!- delinquentme [~delinquen@108-235-112-153.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap 19:48 < mindsForge> that caterpillar is inspiring me to see if I can make a jello mold lol 19:48 < mindsForge> I didnt realize it looked delicious until now 19:48 < mindsForge> https://ultimaker.com/en/stories/19498-using-3d-printed-shapes-to-create-food-molds 19:52 < kanzure> some sort of water bottle alternative thing http://www.oohowater.com/ 19:53 < mindsForge> oo yeah I remember that, neat stuff 19:54 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 20:22 -!- Darius [~quassel@66-215-89-229.dhcp.psdn.ca.charter.com] has joined ##hplusroadmap 20:30 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Quit: WeeChat 1.7.1] 20:33 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 20:41 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Quit: WeeChat 1.7.1] 20:41 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 21:01 -!- mrdata__ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 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##hplusroadmap 22:21 -!- TC [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 22:22 -!- TC is now known as Guest31915 22:22 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 260 seconds] 22:25 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 260 seconds] 22:31 -!- delinquentme [~delinquen@2602:306:ceb7:990:b9b4:dbc:4ee9:786b] has quit [Quit: Leaving] 22:45 < mindsForge> kanzure: https://www.youtube.com/watch?v=fcqzGHI5RUo check this album out, the album art and production quality of your link reminded me of it 22:48 < mindsForge> Interloper is way more chill 22:56 -!- Jenda`_ [~jenda@coralmyn.hrach.eu] has quit [Remote host closed the connection] 23:05 -!- Jenda [~jenda@coralmyn.hrach.eu] has joined ##hplusroadmap 23:06 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 252 seconds] 23:09 -!- traumschule [~traumschu@185.104.184.179] has joined 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