--- Log opened Sat Oct 21 00:00:52 2017
00:07 < nmz787> juul: .wik Serial block-face scanning electron microscopy
00:07 < nmz787> .wik Serial block-face scanning electron microscopy
00:07 < yoleaux> "Serial block-face scanning electron microscopy (SBEM, SBSEM or SBFSEM) is a method to generate high resolution three-dimensional images from small samples. The tehcnique was developed for brain tissue, but it is widely applicable for any biological samples." — https://en.wikipedia.org/wiki/Serial_block-face_scanning_electron_microscopy
00:08 < nmz787> juul: very slow though
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00:58 < kanzure> halcyon wanted to manufacture an army of high-performance scanning electron microscopes
01:21 < juul> nmz787: interesting. thanks!
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01:40 < fenn> .wik ATLUM
01:40 < yoleaux> fenn: Sorry, that command (.wik) crashed.
01:41 < fenn> juul http://cbs.fas.harvard.edu/science/connectome-project/atlum
01:43 < fenn> i really feel that FISSEQ with aptamers for individual brain proteins is the way to go
01:44 < fenn> there are only so many different stains you can use before the colors start to overlap
01:46 < fenn> some evil hybrid between PALM/STORM and FISSEQ would have enough resolution to pick out individual protein molecules while having enough channels to distinguish different types of protein
01:49 < fenn> juul also look into expansion microscopy and CLARITY
01:50 < fenn> .wik CLARITY
01:50 < yoleaux> "CLARITY  is a method of making brain tissue transparent using acrylamide-based hydrogels built from within, and linked to, the tissue, and as defined in the initial paper, represents "transformation of intact biological tissue into a hybrid form in which specific components are replaced with exogenous elements that provide new  …" — https://en.wikipedia.org/wiki/CLARITY
01:52 < fenn> https://nihdirectorsblog.files.wordpress.com/2013/04/hippocampus.jpg
01:53 < fenn> if i murder people who write the wikipedia fair-use image resizer bots, will i be rewarded in the afterlife?
01:55 < fenn> .title http://www.youtube.com/watch?v=c-NMfp13Uug
01:55 < yoleaux> See-through brains - YouTube
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05:20 < kanzure> looks like michel bauwens is still around https://lists.ourproject.org/pipermail/p2p-foundation/2017-October/thread.html
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06:13  * adlai is quite pleased about how the plants that tried replacing plants will eventually be rereplaced with plants
06:14 < kanzure> i think cambrian/austen's idea of replacing all plants was a good idea
06:19 < JayDugger> Good morning.
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06:50 < kanzure> azertyblac0: hi.
06:51 < azertyblac0> hello
06:51 < azertyblac0> first time using IRC
06:53 < kanzure> what brings you here
06:55 < azertyblac0> always to learn
06:55 < ebowden> What's this idea about replacing all plants?
06:58 < azertyblac0> haven't heard that one yet
06:59 < ebowden> kanzure, ^
07:01 < kanzure> ebowden: plants suck so it makes sense to redo their photosystems
07:01 < ebowden> What about their photosystems has been sucessfuly improved?
07:02 < ebowden> It is possible that their limited range of absorbtion may be to avoid overheating.
07:03 < kanzure> "Improving photosynthesis and crop productivity by accelerating recovery from photoprotection" http://science.sciencemag.org/content/354/6314/857
07:03 < ebowden> Ahhh.
07:04 < ebowden> Any other aspects?
07:04 < kanzure> sure. why do you ask?
07:04 < ebowden> Because I am now very curious.
07:04 < kanzure> go find it
07:05 < ebowden> You don't have a list of papers somewhere, like you always seem to?
07:06 < ebowden> kanzure, see if you get this pun:
07:06 < ebowden> The pinnacle of perfection: https://i.pinimg.com/736x/68/50/3a/68503a9246328d9170284002aebc4e05--bassett-hound-ears.jpg
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07:51 < ebowden> kanzure, what baffles me is why evolution has not produced a plant that has fast photoprotection recovery, one would expect it to outcompete other plants without the adaptation.
08:18 < kanzure> fitness landscape has many peaks/valleys that are not feasible to cross without human insight
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08:52 < kanzure> MultiColoredHat: be greeted
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08:55 < MultiColoredHat> @kanzure, hey :D
08:55 < MultiColoredHat> @kanzure, how can i be of asistence :D?
08:58 < kanzure> what are your abilities
09:00 < MultiColoredHat> anything form changing diapers to Armitage, W3af, etc
09:13 < ebowden> kanzure, I'm aware, but this particular case just involved speeding up interconversion.
09:13 < ebowden> My problem is seeing the apparently required dip in the fitness landscape.
09:14 < ebowden> Must be something unusual about the mechanism.
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13:48 < kanzure> http://2017.igem.org/Team:Austin%20UTexas "Since the discovery of the microbiota-gut-brain axis, there has been interest in using foods containing genetically engineered probiotics as alternative forms of medicine. Our project is aimed at designing a probiotic (specifically Lactobacillus plantarum) capable of producing high levels of gamma-aminobutyric acid (GABA) to treat patients with ...
13:48 < kanzure> ...anxiety and bowel disorders. To make our probiotic overproduce GABA, we intend to overexpress gadB, which encodes glutamate decarboxylase, the enzyme that converts L-glutamate into GABA. Overexpression of gadB will be accomplished by placing it under the control of a Lactococcus lactis constitutive promoter. The efficacy of our probiotic in the human gut will be modeled using the gut of Apis ...
13:49 < kanzure> ...mellifera. Additionally, we have created the Broad Host Range Plasmid Kit, a genetic toolset composed of part plasmids that can be quickly assembled into cassette plasmids, which can then be used to easily genetically modify Lactobacillus plantarum and other non-model organisms."
13:51 < kanzure> http://2017.igem.org/Team:BGIC-Union dCasentry: "... our project uses paired dCas9 protein links with split T7 RNA polymerase, guided by SgRNA to carry out the procedure. DCasentry not only deals with low CtDNA concentration in blood but also capable of producing various kinds of signal output. We adopt freeze-dried paper chip as our vector of detecting system to simplify its operation as ...
13:51 < kanzure> ...well as storage. The product will be presented as a kit containing all items necessary for detection and can be widely use on the medical field."
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15:41 < juul> I'll have to look at those casettes
15:42 < kanzure> just doing my igem 2017 review. some of these are not.... not quite english.
15:46 < catern> oh, I had a great idea regarding recording all sound around me
15:46 < catern> I can use a bluetooth headset or something like it!
15:46 < catern> this idea inspired by the bluetooth headphones I recently received as a gift
15:46 < catern> I already plan to wear them all the time, so this is easy
15:47 < kanzure> catern: maaku has been working on 24/7 audio recording wearable devices
15:49 < catern> yeah, I think we talked about it before
15:49 < catern> maaku: made progress?
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16:08 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=73583998 Bryan Bishop: begin processing igem 2017 projects >> http://diyhpl.us/diyhpluswiki/dna/projects/
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17:29 < fenn> hmm
17:29 < fenn> .title http://farmbot.io/
17:29 < yoleaux> Meet FarmBot - Humanity's First Open-Source CNC Farming Machine
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20:11 < kanzure> "Nano-patterning of diffraction gratings on human hair for cosmetic purposes" http://file.scirp.org/pdf/JCDSA_2014052909355620.pdf
20:11 < kanzure> cc nmz787
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23:59 < nmz787> huh
--- Log closed Sun Oct 22 00:00:53 2017