--- Log opened Mon Jan 08 00:00:07 2018
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06:16 < kanzure> .botsnack
06:16 < yoleaux> :D
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06:31 < kanzure> https://www.reddit.com/r/cryonics/comments/5fori9/recent_post_on_new_cryonet_by_mike_darwin/n
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08:17 <@fenn> dropsynth is a smart idea
08:18 <@fenn> i wonder if it can do repeated hierarchical assembly with the barcode beads
08:18 <@fenn> how long of a sequence can you hang off of a bead before it breaks?
08:18 <@fenn> it seems too obvious in hindsight
08:19 < kanzure> trivial to have 1 kbp off of a bead without breakage
08:19 <@fenn> yeah but how big can it go? chromosome assembly?
08:20 <@fenn> assume the barcode is as long as you want
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09:23 < kanzure> music! https://www.youtube.com/watch?v=I3UlCDLXcvc
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10:39 < nmz787> fenn: if you're feeling artsy... a diagram of the dropsynth might be nice... the language as it was written wasn't very easy for me to get a good intuitive understanding of what they're trying to do
10:39 < nmz787> I really got confused when they kept referring to "pull down"
10:52 <@fenn> i just watched the video
10:52 <@fenn> https://vimeo.com/249559350
10:53 <@fenn> "pull down" just means "binds and precipitates out of solution"
10:55 < kanzure> https://www.ldeming.com/longevityfaq
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11:55 < kanzure> dstrbd: hi
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12:15 < dstrbd> hi
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12:21 < kanzure> dstrbd: what brings you here
12:22 < dstrbd> I found the channel topics very interesting
12:23 < dstrbd> I'm a keto stoic software developer
12:23 < kanzure> oh you write software for ketogenesis
12:23 < dstrbd> And also a biohacker :)
12:24 < dstrbd> Not for
12:24 < dstrbd> I write code while in ketosis
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12:24 < dstrbd> How about yourself?
12:26 < kanzure> i am the supreme leader of hplusroadmap
12:26 < kanzure> http://diyhpl.us/wiki/hplusroadmap
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12:47 < razzy> all hail kanzure
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12:57 < dstrbd> amazing kanzure
12:59 < dstrbd> how can I cooperate with?
13:02 < kanzure> what biology things do you know how to do?
13:05 < dstrbd> crispr
13:05 < dstrbd> and some psychedelic related drugs
13:06 < dstrbd> intensive nootropics usage also
13:06 < dstrbd> and open source hacking culture
13:07 < kanzure> well, hmmm
13:07 < kanzure> one of our projects-
13:07 < kanzure> https://blog.kitmatic.com/2017/06/28/electroporation-is-now-quick-high-yield-and-commodity-priced/
13:07 < kanzure> https://github.com/kanzure/culture_shock
13:11 < dstrbd> cool
13:11 < dstrbd> thanks for sharing
13:11 < dstrbd> i will check it out more carefully soon
13:21 < MrHindsight> are there any thermocyclers on the market that use microwaves for heating the samples?
13:22 < kanzure> wouldn't that be overkill? you just need a simple heating element..
13:22 < MrHindsight> need for speed
13:23 < MrHindsight> PCR in minutes™
13:23 < kanzure> some of the thermocyclers advertise that
13:24 < kanzure> openpcr is not so good
13:24 < kanzure> a friend of ours was working on "lava amp", which was a microfluidic PCR setup in a ring/circle
13:24 < kanzure> "Low cost approach to PCR" http://diyhpl.us/~bryan/papers2/bio/Low%20cost%20approach%20to%20PCR%20-%202007.pdf
13:26 < MrHindsight> too slow and only for those tiny samples you are used to
13:29 < MrHindsight> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671502/
13:30 < kanzure> .title
13:30 < yoleaux> Fragmentation of Genomic DNA using Microwave Irradiation
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14:09 <@fenn> the challenge is cooling quickly
14:09 <@fenn> i like idaho's glass capillary PCR tube design
14:10 <@fenn> it's just a light bulb and a fan, but manages to outdo all the fancy stuff in terms of heating and cooling rates, because the sample tubes have a very high surface area and are directly heated/cooled by the medium, instead of some heat transfer block
14:11 <@fenn> it is a glass tube about 1mm diameter and you melt the ends shut with a bunsen burner to close it
14:12 <@fenn> also there's no lid for water to condense on, so the concentration stays the same throughout the cycle
14:16 < MrHindsight> they assume small samples, mine are >1L ea
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14:28 < MrHindsight> http://www.interplas.com/product_images/ziplock-bags/Leakproof-Ziplock-Bags-1000px.jpg
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14:42 <@fenn> yeah for that i'd do a thin tube wrapped in a figure 8 around heat transfer cylinders and do a continuous process
14:43 <@fenn> or just ghetto style water baths
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14:49 <@fenn> jeez it seems DNA is damaged by just about every metal i'd want to use for the tubing
14:52 <@fenn> maybe internally gold plated copper tubing would work
14:54 <@fenn> or just a really thin polymer coating
14:59 < kanzure> isn't there some sort of inductive anti-heating thing... uh.
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15:04 <@fenn> you could put some magnetocaloric beads in solution, but they aren't really very good
15:07 <@fenn> like less than 1 degree of cooling
15:07 < kanzure> whatcha doing againN?
15:10 <@fenn> he's under NDA but something to do with "fragments"
15:12 < nmz787> call with Karin Strauss this or next week
15:18 < kanzure> .wik COX7A1
15:19 < yoleaux> "Cytochrome c oxidase polypeptide 7A1, mitochondrial is an enzyme that in humans is encoded by the COX7A1 gene." — https://en.wikipedia.org/wiki/COX7A1
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16:09 < MrHindsight> fenn:  can't do continuous since every batch is different
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16:42 < MrHindsight> 1L disposable silly straws
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17:02 < kanzure> MrHindsight: are there variable volume nozzles that can be controlled electronically?
17:08 < kanzure> longevity fund stuff https://news.ycombinator.com/item?id=16099448
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17:28 < kanzure> brujo_bi1logica: hi.
17:31 < MrHindsight> kanzure: sort of, multiple drops that combine into one while still in flight
17:31 < MrHindsight> https://inkjetinsight.com/knowledge-base/understanding-gray-areas-inkjet/
17:33 < brujo_bi1logica> whatsup kanzure
17:34 < brujo_bi1logica> getting a logic analyzer to dive into that ABI 391 cartridge
17:39 < kanzure> MrHindsight: thanks
17:39 < kanzure> brujo_bi1logica: cool, are you going to dump the ROM? i'd love to reverse engineer it.
17:40 < MrHindsight> kanzure: don't expect much and keep it simple
17:41 < MrHindsight> inkjet is centered around making money selling fluids, not actually providing the world with deposition equipment
17:41 < kanzure> heh
17:42 < kanzure> well that's not good
17:42 < kanzure> deposition is very useful
17:42 < MrHindsight> I happen to know the ins and outs since I mostly deal with all the oddball stuff
17:43 < MrHindsight> the makers of inkjet technology are only interested in profit
17:44 < MrHindsight> they don't care if anyone lives or dies, is happy or sad, sick or well,  as long as they make a profit
17:44 < brujo_bi1logica> im going to practice on old routers and cartridges
17:45 < brujo_bi1logica> i need to figure out the pinout and not damage the card
17:45 < brujo_bi1logica> *cartridge
17:48 < MrHindsight> https://www.theverge.com/2012/3/17/2879235/clemson-researchers-how-to-print-cells-hp-inkjet
17:48 < MrHindsight> the author sounds familiar
17:53 < kanzure> theverge.com has an author named "bryan bishop" it's not me
17:53 < kanzure> there's also another one running around called "bald bryan" because he had cancer and i can't get rid of him because people are sympathetic to cancer survivors
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18:14 < juul> At least you don't have an e-cig product as name collision :/
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18:36 < nmz787> gmail can't search $
18:39 < nmz787> we seriously need a new search engine revolution
18:39 < kanzure> grep
18:39 < nmz787> well that doesn't work unless I download all my email RIGHT NOW
18:40 < nmz787> which is going to take a lot longer than having better cloud tools
18:40 < nmz787> (would make it)
18:46 < kanzure> most people download their email every day
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18:54 < nmz787> not us cloud people
18:54 < nmz787> we're like buoyant ewoks
19:07 < nmz787> brujo_bi1logica: ABI 391 "cartridge" ?
19:08 < nmz787> you mean the ICs? or chemical/reagent cartridge?
19:13 < nmz787> brujo_bi1logica: have you seen my (now out of order) pics? http://diyhpl.us/~nmz787/abi391.html
19:14 < nmz787> brujo_bi1logica: I'm guessing you mean this https://s3.amazonaws.com/s3.takeitapart.com/userimages/RnMXnoY.jpg
19:15 < kanzure> ttp://diyhpl.us/~bryan/papers2/DNA/abi391/
19:15 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/abi391/
19:15 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/abi391/takeitapart/
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19:21 < nmz787> kanzure: you could move that HTML into your folder... I just made it just now with the list you had sitting in there
19:26 < kanzure> lightning network stuff https://explorer.acinq.co/
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20:16 < brujo_bi1logica> nmz787: kanzure: I have the os v1.0.0 cartridge ;D
20:17 < brujo_bi1logica> its fairly useless otherwise and don't seem to be readily available
20:18 < brujo_bi1logica> hence i gotta be fragile
20:18 < kanzure> have you used jtag before?
20:21 < MrHindsight> why do you want to RE that old thing?
20:27 < MrHindsight> it's just a bunch of solenoid valves and controller/drives for the solenoids
20:44 < kanzure> just curiosity
20:45 < kanzure> nothing else
20:45 < MrHindsight> really not much there
20:49 < MrHindsight> http://diyhpl.us/~nmz787/abi391.html just look at the part number on the logic
20:49 < MrHindsight> number/s
21:07 < Hooloovo0> cover back up that ROM chip's window
21:07 < Hooloovo0> it'll probably be fine otherwise
21:07 < Hooloovo0> but still
21:07 < Hooloovo0> you can get eprom programmers that can read those
21:08 < Hooloovo0> I use a TL866
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21:28 < juul> https://www.youtube.com/watch?v=iBk8owuhNkQ
21:31 < kanzure> title
21:31 < kanzure> .tilte
21:31 < kanzure> .TI TLE
21:31 < kanzure> .title
21:31 < yoleaux> Crypt Of The Necrodancer OST - Danny Baranowsky - full album (2015) - YouTube
21:32 < kanzure> not bad https://www.youtube.com/watch?v=iBk8owuhNkQ&t=10m25s
21:45 < kanzure> .g "electronic biosciences"
21:45 < yoleaux> http://ipv6.google.com/sorry/index?continue=http://www.google.com/search%3F%26q%3D%2522electronic%2520biosciences%2522%26btnI%3D&q=EhAqAQT4AgFyMAAAAAAACGrnGOet0dIFIhkA8aeDS_A3xPn7FEz2NciTBDtiHUNRlsjUMgFy
21:51 < kanzure> https://www.fightaging.org/archives/2017/05/planning-a-single-person-trial-of-senolytic-drug-candidates/
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--- Log closed Tue Jan 09 00:00:07 2018