--- Log opened Fri Jan 12 00:00:10 2018 00:08 < nmz787> fenn: if you were to add a Z to the laser_etcher CAD you designed, how would you do it? 00:12 < archels> Reversible Destiny Statute: "Not making an all-out effort to go on living and the act of dying are from this date on classed first-order felonies. Citizens will need to strive to define the heartiness of their existences and be responsible for astute and timely assessment of negative patterns of events and failed or failing conditions. Choosing to live within a tactically posed surround/tutelary abode will be counted as an all-out effort to ... 00:12 < archels> ... go on living." 00:16 -!- jrayhawk [~jrayhawk@unaffiliated/jrayhawk] has quit [Quit: leaving] 00:38 -!- augur [~augur@192.195.83.130] has quit [Remote host closed the connection] --- Log opened Fri Jan 12 00:49:31 2018 00:49 -!- gnusha [~gnusha@unaffiliated/kanzure/bot/gnusha] has joined ##hplusroadmap 00:49 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | as investigated by the fbi weapons of mass destruction division | this channel is LOGGED: http://gnusha.org/logs | http://diyhpl.us/wiki | "ray kurzweil is a pessimist" - george church 00:49 -!- Topic set by kanzure [~kanzure@unaffiliated/kanzure] [Tue Aug 29 14:28:51 2017] 00:49 [Users ##hplusroadmap] 00:49 [ adlai ] [ dustinm- ] [ juul ] [ rancyd_ ] 00:49 [ aeiousomething ] [ ebowden ] [ kuldeep ] [ razzy ] 00:49 [ andytoshi ] [ eleitl ] [ maaku ] [ red-001 ] 00:49 [ anonnumberanon ] [ EnabrinTain ] [ Madars ] [ redlegion ] 00:49 [ archels ] [ ensign ] [ mf1008 ] [ rk[ghost] ] 00:49 [ Avian-Anathema_] [ entity8421[m] ] [ mgxm ] [ s0ph1a ] 00:49 [ balrog ] [ ExeciN ] [ midnightmagic] [ sachy ] 00:49 [ bbrittain ] [ fltrz ] [ mrdata ] [ sandeepkr ] 00:49 [ berndj ] [ FourFire ] [ Mrkva ] [ saturn2 ] 00:49 [ bkero ] [ gnusha ] [ namespace ] [ saurik ] 00:49 [ BobaMa ] [ gwillen ] [ nanotube ] [ sivoais ] 00:49 [ brujo_bi1logica] [ heath ] [ nickjohnson ] [ streety ] 00:49 [ brujo_biologica] [ hehelleshin ] [ night ] [ superkuh ] 00:49 [ Burninate ] [ HEx1 ] [ NikopolSohru ] [ Taek ] 00:49 [ CaptHindsight ] [ Hooloovo0 ] [ nmz787 ] [ thundara_ ] 00:49 [ catern ] [ indiebio_ ] [ nsh ] [ TMA ] 00:49 [ Cory ] [ Iriez ] [ pasky ] [ traumschule] 00:49 [ darsie ] [ JayDugger ] [ pepesza ] [ vikraman ] 00:49 [ DataPacRat ] [ Jenda` ] [ pompolic ] [ y0no ] 00:49 [ dequ_ ] [ Joshchamp_ ] [ potatope ] [ yoleaux ] 00:49 [ docl ] [ juri_ ] [ Qfwfq ] [ yorick ] 00:49 [ Douhet ] [ justanotheruser] [ rancyd ] 00:49 -!- Irssi: ##hplusroadmap: Total of 87 nicks [0 ops, 0 halfops, 0 voices, 87 normal] 00:49 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 00:49 -!- Irssi: Join to ##hplusroadmap was synced in 14 secs 00:53 -!- jrayhawk [~jrayhawk@unaffiliated/jrayhawk] has joined ##hplusroadmap 02:08 -!- ebowden [~ebowden@110.141.70.22] has quit [Ping timeout: 264 seconds] 02:09 -!- ebowden [~ebowden@110.141.70.22] has joined ##hplusroadmap 02:12 < TMA> archels: what kind of tutelary abode is adequate under that statute? any manmade shelter is an instance of a "tutelary abode" 02:25 -!- NikopolSohru [~NSohru@89.238.185.110] has quit [Quit: Leaving] 02:31 -!- kanzure [~kanzure@unaffiliated/kanzure] has joined ##hplusroadmap 02:43 -!- drewbot [~cinch@54.90.140.128] has joined ##hplusroadmap 02:44 < kanzure> "Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379551/ 02:46 < kanzure> .tw https://twitter.com/TinyFPGA/status/951676966226223105 02:46 < yoleaux> Video of @latticesemi iCE40 FPGA being reflowed onto custom footprint. can't see the solder balls at the point they reflow, but you can see the package self-align very strongly. Far side reflows first tipping up near side before it pulls down flat and to the left. https://video.twimg.com/ext_tw_video/951676594657017856/pu/pl/MSVaLwa7P8EOWJN_.m3u8 (@TinyFPGA) 02:52 < kanzure> .tw https://twitter.com/eboyden3/status/951146281468153856 02:52 < yoleaux> The optogenetic molecule Chrimson (https://www.nature.com/articles/nmeth.2836) is headed for human trials, with the first patient scheduled for treatment in the first quarter of 2018. https://www.businesswire.com/news/home/20180110005459/en/GenSight-Biologics-Receives-MHRA-Approval-Initiate-Phase (@eboyden3) 02:52 -!- Gurkenglas [~Gurkengla@dslb-094-223-133-187.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 03:11 -!- jtimon [~quassel@41.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 03:52 -!- bluebear_ [~dluhos@80.95.97.194] has joined ##hplusroadmap 05:09 -!- DataPacRat [~dan@184.75.221.130] has quit [Ping timeout: 264 seconds] 06:42 < archels> TMA: apparently these guys designed and built some really quirky houses. rooms where not a single part of the floor was level, organic shapes, etc. 06:43 < archels> it's probably mostly about artistic expression, but making death illegal is a cute theme for a sci-fi fiction, anyways 06:51 < kanzure> "Acoustically targeted chemogenics for noninvasive control of neural circuits" https://www.biorxiv.org/content/biorxiv/early/2018/01/01/241406.full.pdf 06:51 < kanzure> "Controlled placement of multiple CNS cell populations to create complex neuronal cultures" http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0188146 06:53 < kanzure> *chemogenetics 06:53 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-ttrchgynlbbxphoo] has joined ##hplusroadmap 07:00 < kanzure> http://mitbiohackers.com/ 07:07 < kanzure> "Exploring protein orthogonality in immune space: a case study with AAV and Cas9 orthologs" https://www.biorxiv.org/content/early/2018/01/10/245985 https://twitter.com/Alexis_Verger/status/951368309999587328 07:07 < kanzure> "One method of circumventing these issues is to use human or humanized proteins which avoid the immune response by self-recognition. However, this approach limits potential protein therapeutics to those of human origin, excluding many exciting effectors and delivery vehicles such as CRISPR-Cas9 and adeno-associated viruses (AAVs). To address this issue, we propose here the sequential use of ... 07:07 < kanzure> ...orthologous proteins whose function is constrained by natural selection, but whose structure is subject to diversification by genetic drift. This would, in principle, allow for repeated treatments by immune orthogonal orthologs without reduced efficacy due to lack of immune cross-reactivity among the proteins. To explore and validate this concept we chose 91 Type II CRISPR-Cas9 orthologs and ... 07:07 < kanzure> ...167 AAV capsid protein orthologs, and developed a pipeline to compare total sequence similarity as well as predicted binding to class I and class II Major Histocompatibility Complex (MHC) proteins. Interestingly, MHC binding predictions revealed wide diversity among the set of Cas9 orthologs, with 83% of pairs predicted to have non cross-reacting immune responses, while no global immune ... 07:07 < kanzure> ...orthogonality among AAV serotypes was observed." 07:09 < kanzure> .tw https://twitter.com/koeng101/status/951527617479389184 07:09 < yoleaux> Conary Meyer finished automating transformations and plating of E. coli with a #opentrons ! We’re still cleaning up the code, but you can check it out here - https://github.com/EndyLab/bionet-synthesis/tree/master/pipeline https://pbs.twimg.com/media/DTSBUV0VoAAJoQB.jpg (@koeng101) 07:11 < kanzure> .tw https://twitter.com/koeng101/status/951527991334457344 07:11 < yoleaux> Btw, it works by doing a 4 fold serial dilution on 15ul of competent E. coli transformed with 1ul of GoldenGate reaction. (@koeng101, in reply to tw:951527617479389184) 07:14 < kanzure> http://effective-altruism.com/ea/1iu/2018_ai_safety_literature_review_and_charity/ 07:15 < kanzure> "Survival time of Princess Kaguya in an air-tight bamboo chamber" https://arxiv.org/abs/1712.05909n 07:16 < kanzure> .tw https://twitter.com/anderssandberg/status/946522763224862720nnnn 07:16 < yoleaux> Och, A cannae finde nae tweet 07:16 < kanzure> .tw https://twitter.com/anderssandberg/status/946522763224862720 07:16 < yoleaux> Interesting: DBS rewards can make mice use rules to avoid conflict and maximize mutual benefit, something that does not happen with food rewards. Looks wonderfully non-zerosum. https://www.nature.com/articles/d41586-017-08835-9 https://www.nature.com/articles/s41467-017-01091-5 (@anderssandberg) 07:51 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 07:54 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has joined ##hplusroadmap 08:11 < kanzure> "Active intracellular delivery of Cas9-sgRNA complex using ultrasound-propelled nanomotors" http://onlinelibrary.wiley.com/doi/10.1002/anie.201713082/abstract 08:26 < kanzure> "Single phage T4 DNA packaging motors exhibit large force generation, high velocity, and dynamic variability" http://www.pnas.org/content/104/43/16868 08:29 < kanzure> "Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture" https://www.nature.com/articles/srep31516 08:29 < kanzure> https://joinmarket.me/blog/blog/flipping-the-scriptless-script-on-schnorr/ 08:29 < kanzure> https://joinmarket.me/blog/blog/bulletpoints-on-bulletproofs/ 08:39 -!- eudoxia [~eudoxia@190.99.101.24] has joined ##hplusroadmap 08:54 -!- eudoxia [~eudoxia@190.99.101.24] has quit [Quit: Leaving] 09:01 -!- Gurkenglas [~Gurkengla@dslb-094-223-133-187.094.223.pools.vodafone-ip.de] has quit [Ping timeout: 260 seconds] 09:04 -!- m4l3z [~m4l3z@2a01cb040226f200268eaf478c039961.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap 09:04 -!- m4l3z [~m4l3z@2a01cb040226f200268eaf478c039961.ipv6.abo.wanadoo.fr] has left ##hplusroadmap [] 09:13 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-ttrchgynlbbxphoo] has quit [Quit: Connection closed for inactivity] 09:38 < kanzure> parenting stuff - https://news.ycombinator.com/item?id=16132955 09:38 < kanzure> 1) if you don't feel comfortable raising another guy's kids (e.g. your partner had children with someone else), then you shouldn't want to just gamble on the genetic lottery in the first place, even if they are biologically your kids 09:38 < kanzure> 2) i think we can do a simple study of babies and screaming and complications during early childhood, and then propose some mutations that seem to be correlated with less screaming and a more pleasant parenting experience 09:50 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has quit [Quit: dstrbd] 10:01 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has joined ##hplusroadmap 10:02 < kanzure> i wonder if fusion proteins are detectable by human immune system 10:03 < kanzure> analyzing cas9 variants for structures that more similarly match humanized proteins is a neat trick, but not if you can just stick a cas9 to a humanized protein and be totally done with the problem anyway 10:09 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has quit [Quit: dstrbd] 10:24 -!- DataPacRat [~dan@adsl-137.itcanada.com] has joined ##hplusroadmap 10:25 -!- nmz787_ [~nmz787@bryan.fairlystable.org] has joined ##hplusroadmap 10:31 -!- augur [~augur@2600:380:8664:62d4:e1e8:bf6c:39a3:666a] has joined ##hplusroadmap 10:33 -!- fenn [~fenn@unaffiliated/fenn] has joined ##hplusroadmap 10:34 < kanzure> be greeted 10:34 -!- Netsplit *.net <-> *.split quits: saturn2 10:35 -!- saturn2 [~visitant@unaffiliated/clone-of-saturn/x-2509460] has joined ##hplusroadmap 10:36 < kanzure> "Deep image reconstruction from human brain activity" https://www.biorxiv.org/content/biorxiv/early/2017/12/30/240317.full.pdf 10:36 -!- yoleaux [~yoleaux@xn--ht-1ia18f.nonceword.org] has quit [Ping timeout: 240 seconds] 10:37 -!- yoleaux [~yoleaux@xn--ht-1ia18f.nonceword.org] has joined ##hplusroadmap 10:57 < kanzure> .title https://www.youtube.com/watch?v=nso4tDuYdm8 10:57 < yoleaux> Solvespace: Geneva Drive Mechanism. Design and 3D print. - YouTube 11:27 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has joined ##hplusroadmap 11:32 -!- bluebear_ [~dluhos@80.95.97.194] has quit [Quit: Leaving.] 11:33 -!- DataPacRat [~dan@adsl-137.itcanada.com] has quit [Ping timeout: 265 seconds] 11:34 -!- DataPacRat [~dan@vdsl-33.itcanada.com] has joined ##hplusroadmap 11:38 -!- fltrz [d5db9402@gateway/web/freenode/ip.213.219.148.2] has quit [Ping timeout: 260 seconds] 11:39 < kanzure> cas9 orthologs http://www.orthodb.org/?query=CRISPR%20associated%20protein%209 11:40 < kanzure> oh the text search sucks O_O it's just matching words in the names of other proteins wtf 11:41 < kanzure> that's... not an ortholog. 12:00 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Ping timeout: 248 seconds] 12:02 -!- rpifan [~rpifan@172.56.26.218] has joined ##hplusroadmap 12:04 -!- rpifan [~rpifan@172.56.26.218] has quit [Read error: Connection reset by peer] 12:25 -!- MrHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap 12:27 -!- aeiousom1thing [~aeiousome@183.82.170.54] has joined ##hplusroadmap 12:28 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Ping timeout: 260 seconds] 12:28 -!- MrHindsight is now known as CaptHindsight 12:28 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 248 seconds] 13:15 < kanzure> .wik memjet 13:15 < yoleaux> "Memjet is a printing technology and components company. It maintains its corporate office in San Diego, California, and has offices in Dublin, Sydney, Taipei, Singapore and Boise, Idaho. Memjet's president and chief executive officer, Len Lauer, was appointed in January 2010." — https://en.wikipedia.org/wiki/Memjet 13:17 < CaptHindsight> yeah secret co 13:17 < CaptHindsight> they only sell a few MEMS printers 13:18 < CaptHindsight> don't like talking to people 13:20 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has quit [Quit: dstrbd] 13:46 -!- augur [~augur@2600:380:8664:62d4:e1e8:bf6c:39a3:666a] has quit [Ping timeout: 252 seconds] 14:17 < kanzure> .title https://www.youtube.com/watch?v=vx4-HZ0pfgE&list=UUTiPTTofN814JKU0e7eNkLA&index=4 14:17 < yoleaux> Phages T5 ejecting their DNA in a giant lipid vesicle - YouTube 14:37 -!- strages [uid11297@gateway/web/irccloud.com/x-wuelhbhrxakxplin] has joined ##hplusroadmap 14:39 -!- DataPacRat [~dan@vdsl-33.itcanada.com] has quit [Ping timeout: 256 seconds] 15:11 -!- berndj [~berndj@mail.azna.co.za] has quit [Quit: ZNC - http://znc.in] 15:13 -!- berndj [~berndj@mail.azna.co.za] has joined ##hplusroadmap 15:19 < kanzure> maaku: what are your best arguments for the highest conceivable economic value of strong atomic molecular nanotechnology? 15:21 < kanzure> i've actually been asked this about de novo biological dna synthesis, and i think it essentially boils down to "all of molecular nanotechnology" 15:22 < kanzure> people sometimes argue to me that the value of dna synthesis is actually low because once you have the molecule then you can just copy it infinitely, but that's like telling me that once you have software written it just works forever (but we have bit rot or something) 15:22 < kanzure> well, i guess the total bootable system (cells) are easy to store, so i guess it might not really require that much ongoing maintenance 15:25 < kanzure> it's really weird that dna synthesis machines were originally sold to labs instead of starting with centralized facilities... the economics didn't favor selling machines back then. so why? 15:27 < kanzure> https://diyhpl.us/wiki/nanotech/molecular-manufacturing/ 15:49 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has joined ##hplusroadmap 16:19 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has left ##hplusroadmap ["Leaving"] 16:45 -!- augur [~augur@192.195.83.130] has joined ##hplusroadmap 16:55 < maaku> not my best ansewr, but off-the-cuff the economic value of strong atomic molecular nanotechnology is at least equal to the non-service, non-food portion of global GDP 16:57 < maaku> If you think of current manufacturing as top-down refinement of crude materials requiring regional distribution of materials, atomicly precise manufacturing is bottom-up manufacturing done with local materials 16:59 < maaku> And whereas present manufacturing has high costs, atomicly precise manufacturing should have costs on the order of biology -- making computer chips for the same cost as potatos 17:01 < maaku> The more involved answer requires looking at what diamondoid materials and "smart materials" with built-in mechanisms like atomic pumps can provide in terms of material properties 17:02 < maaku> Since you can get orders of magnitude higher strenghts at an order of magnitude lower density, that alone opens up vast industrial potential. 17:03 < maaku> And the fact that you can build in selective sort and pump mechanisms, embedded computation, etc. 17:03 < maaku> I imagine that Merkle, Freitas, Drexler have done more analysis of this than I have. 17:04 -!- rpifan [~rpifan@172.56.26.84] has joined ##hplusroadmap 17:05 -!- rpifan [~rpifan@172.56.26.84] has quit [Max SendQ exceeded] 17:56 -!- dstrbd [~dstrbd@ec2-52-90-70-199.compute-1.amazonaws.com] has quit [Quit: dstrbd] 19:01 -!- jtimon [~quassel@41.31.134.37.dynamic.jazztel.es] has quit [Read error: Connection reset by peer] 19:31 < nmz787_> kanzure: I think one more extension to your copying-DNA is like copying-Software isn't about bitrot... but more that this software can not take on some vastly new functionality... you either hack that code, or rewrite the source 20:05 -!- nmz787 [~nmz787@bryan.fairlystable.org] has left ##hplusroadmap [] 20:06 -!- nmz787_ is now known as nmz787 20:16 < kanzure> maaku: economic analysis does not show up in their books tho 20:37 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 20:38 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 20:38 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 256 seconds] 20:52 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 20:55 -!- aeiousom1thing [~aeiousome@183.82.170.54] has quit [Ping timeout: 255 seconds] 21:04 -!- rpifan [~rpifan@172.56.26.236] has joined ##hplusroadmap 21:05 -!- rpifan [~rpifan@172.56.26.236] has quit [Max SendQ exceeded] 21:06 -!- rpifan [~rpifan@172.56.26.236] has joined ##hplusroadmap 21:07 -!- rpifan [~rpifan@172.56.26.236] has left ##hplusroadmap [] 21:08 -!- augur [~augur@192.195.83.130] has quit [Remote host closed the connection] 21:15 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 248 seconds] 21:21 -!- ebowden [~ebowden@110.141.70.22] has quit [Ping timeout: 255 seconds] 21:21 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 21:25 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 268 seconds] 21:47 -!- ebowden [~ebowden@110.141.70.22] has joined ##hplusroadmap 21:49 < kanzure> polymerase animations http://diyhpl.us/~bryan/papers2/polymerase/molecular-events/ 21:50 < kanzure> assembled from analysis of protein databank snapshots etc 21:50 < kanzure> (not my work, it's from the "molecular events" paper from 2016, from its powerpoint files) 21:53 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 22:13 -!- maaku [~maaku@173.234.25.100] has quit [Quit: ZNC - http://znc.in] 22:16 -!- maaku [~maaku@173.234.25.100] has joined ##hplusroadmap 22:29 -!- drewbot [~cinch@54.90.140.128] has quit [Remote host closed the connection] 22:34 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Read error: Connection reset by peer] 22:35 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 22:36 -!- rpifan [~rpifan@172.56.26.110] has joined ##hplusroadmap 22:38 -!- rpifan [~rpifan@172.56.26.110] has left ##hplusroadmap [] 22:39 < kanzure> yerp.. 23:04 < kanzure> hmph 23:23 -!- ebowden [~ebowden@110.141.70.22] has quit [Ping timeout: 248 seconds] 23:36 -!- DataPacRat [~dan@vdsl-33.itcanada.com] has joined ##hplusroadmap 23:45 -!- DataPacRat [~dan@vdsl-33.itcanada.com] has quit [Read error: Connection reset by peer] 23:46 -!- DataPacRat [~dan@vdsl-33.itcanada.com] has joined ##hplusroadmap 23:58 -!- ebowden [~ebowden@110.141.70.22] has joined ##hplusroadmap --- Log closed Sat Jan 13 00:00:22 2018