--- Log opened Wed Jan 24 00:00:32 2018
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00:56 < archels> "Leeches have two hearts, which are formed of tubes that run along the entire length of the body, one on each side of the animal. Moreover, these two hearts beat with different patterns. Driven by the CPG components, the tube on one side constricts and relaxes back-to-front, while the other side contracts all at once. Every few minutes, the tubes swap patterns."
00:57 < archels> "[...], which suggests that the two sides of a single animal are no more similar to one another than two randomly chosen individuals."
00:57 < archels> more https://elifesciences.org/articles/34153
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06:36 < kanzure> heh strange to see how people sell me to tim ferriss https://twitter.com/RichardHeartWin/status/956085269295456257
06:37 < kanzure> .tw https://twitter.com/hlntnr/status/956004245697736704
06:37 < yoleaux> Surprised/impressed by the level of detail in China's 3-year action plan on AI, published in December - highly specific target specs of drones, sensors, and other tech they want to exist by 2020. (screenshots from Google translate; original here: http://xxgk.miit.gov.cn/gdnps/wjfbContent.jsp?id=5960820) https://pbs.twimg.com/media/DURohg2UQAAPCjA.jpg https://pbs.twimg.com/media/DURohpgVoAAeM_z.jpg (@hlntnr)
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06:39 < kanzure> .tw https://twitter.com/kakape/status/954485560084660224
06:39 < yoleaux> The #horsepox experiment that I reported on this summer was published today. Many researchers think it should not have been. “It was a serious mistake to publish this work”, says @T_Inglesby. “The world is now more vulnerable to smallpox.” http://www.sciencemag.org/news/2018/01/paper-showing-how-make-smallpox-cousin-just-got-published-critics-wonder-why (@kakape)
06:40 < kanzure> .tw https://twitter.com/HankGreelyLSJU/status/955320162760015872
06:40 < yoleaux> Useful post by @pknoepfler on the CRISPR to turn fibroblasts into iPSCs. "Overall, I liked this paper for its fairly novel technical approach, its potential to open the door to a new method of non-genetic reprogramming, and insights into pluripotency." https://ipscell.com/2018/01/crispr-to-make-ips-cells-review-of-new-ding-lab-paper/ (@HankGreelyLSJU)
06:40 < kanzure> "Digitization of multistep organic synthesis in reactionware for on-demand pharmaceuticals" http://science.sciencemag.org/content/359/6373/314
06:41 < kanzure> "This in turn guides production of a three-dimensional printed device that encloses the entire synthetic route internally via simple operations. We demonstrate the approach for the γ-aminobutyric acid receptor agonist, (±)-baclofen, establishing a concept that paves the way for the local manufacture of drugs outside of specialist facilities.
06:41 < kanzure> "
06:42 < kanzure> .tw https://twitter.com/jubersax/status/954836638055591936
06:42 < yoleaux> @rob_carlson Twist pricing for gene synthesis went up by 16% in 2018. Same per bp price ($0.09/bp) and added $25/gene cloning charge. Took the average to $0.105/bp. Still the best deal in town. (@jubersax, in reply to tw:954823530540974080)
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08:19 < kanzure> kriorus cryonics ICO thing https://en.cryogen.me/
08:23 < kanzure> weird choice to make it merely a discount mechanism on purchasing their services.
08:23 < kanzure> i guess that's the best idea they had?
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08:53 < spurserh_> wow, this compressive utlrasound imaging idea is really clever
08:59 < spurserh_> ( https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724356/ )
09:02 < kanzure> nmz787: ok instead of vegan carnivorous plants, i think (besides electroporator testing) i should tell them to make a plasmid that if delivered to humans increases efficiency of future gene therapy attempts (e.g. maybe one of the natural DNA competence proteins but combined with a system that has to be explicitly chemically triggered)
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12:39 < kanzure> "one of the natural DNA competence proteins but combined with a system that has to be explicitly chemically triggered"
12:39 < kanzure> could do TET-inducible expression of the competence DNA pore stuff
12:39 < kanzure> unfortunately the proteins will probably stick around for the rest of the cell cycle or until the pore proteins are degraded
12:40 < kanzure> so you might be adopting random naked DNA.... may not be a big problem in practice. not sure.
12:40 < kanzure> kelu124: i only see ~50 results for "chaotic lens" and "chaotic lense" on google scholar. is this known by a different name?
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14:33 < kanzure> bitcoin security conference talks https://cyber.stanford.edu/bpase18
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15:27 < streety> With the ICO above, is "cryonized" aterm being widely used?
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15:34 < kanzure> don' think so
15:34 < kanzure> don't
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16:05 < streety> seemed weird with "cryopreserved" used on the next line
16:05 < streety> seems more common
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16:43 < JayDugger> Heh. Sure, why not interview with Tim Ferriss?
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19:10 < kanzure> yashgaroth: the competence dna pore protein stuff is like ~40 genes i think. i dunno if there's a smaller system discovered yet. (not to mention questions about compatibility etc.)
19:10 < kanzure> i might have to settle for nuclear localization tag stuff :-(
19:11 < yashgaroth> better that nothing, and decently easy to test in vitro
19:23 < kanzure> yashgaroth: have you played around with a culture shock electroporator yet? https://github.com/kanzure/culture_shock
19:23 < yashgaroth> nope
19:25 < kanzure> dual-syringe injector, what does this look like?
19:26 < yashgaroth> two syringes in a carriage so that they inject continuously while being inserted, distributing plasmid evenly along the length of the needle
19:27 < kanzure> is off-the-shelf thing?
19:27 < yashgaroth> then you connect the needles to electrodes
19:27 < yashgaroth> oh no, there was a paper I'll go find it
19:28 < kanzure> nuclear localization tag is probably fine for now. in future we can move on to super dna pores toggled by chemicals/whatever.
19:29 < yashgaroth> ah it was from inovio, figures https://sci-hub.la/10.1016/j.vaccine.2005.08.068
19:30 < fenn> re: "chaotic lens" they were referring to the pseudorandom phase shift caused by the plate with variable thickness
19:30 < fenn> in the article it's called an aperture mask
19:31 < fenn> i think "compressed sensing" is a bad name
19:33 < fenn> or perhaps people are using it to refer to a variety of related techniques
19:36 < kanzure> hmm https://scholar.google.com/scholar?cites=6997944844782774853&as_sdt=5,44&sciodt=0,44&hl=en
19:36 < fenn> on a different note, why can't you use fluorescent hybridization probes to do preimplantation genetic typing?
19:36 < fenn> .title
19:36 < yoleaux> Sorry...
19:36 < fenn> me too
19:37 < fenn> articles citing "Tjelle: A novel electroporation device for gene delivery in large animals and humans"
19:37 < kanzure> is preimplantation genotyping destructive in any way?
19:38 < yashgaroth> to the cell you pull out for sequencing it is
19:38 < kanzure> oh it's zygote stuff?
19:38 < yashgaroth> mhm
19:38 < kanzure> wait sorry
19:38 < kanzure> i mean to ask, is it the post-fertilization multi-cellular lifeform and you pick one of the cells?
19:38 < yashgaroth> fenn FISH is usually cells that have already been fixed I think
19:39 < yashgaroth> yes
19:39 < fenn> oh, right. so this is what you were going to do anyway
19:39 < kanzure> if you keep picking off cells at about the same rate as they are replicating then do you have an immortal embryo?
19:39 < fenn> depends whether you believe in the hayflick limit or not
19:40 < kanzure> uh let's test it
19:40 < fenn> what, do science? madness!
19:42 < kanzure> "Applicator and electrode design for in vivo DNA delivery by electroporation" https://link.springer.com/protocol/10.1007/978-1-59745-194-9_3
19:43 < kanzure> tjelle human electroporation clinical protocol stuff https://link.springer.com/protocol/10.1007/978-1-59745-194-9_39
19:43 < fenn> embryonic stem cells express telomerase, so presumably they could be an immortal cell line...
19:47 < fenn> it would be a lot faster to do genotyping on all the cells, that way you don't have to manipulate them at all
19:48 < fenn> would a single fluorescent molecule be bright enough to detect?
19:48 < kanzure> i believe that's what all the zeptoliter waveguide polymerase sequencing-by-synthesis stuff is, ya?
19:48 < fenn> a bound probe would tend to stay in the same location more than a freely floating probe
19:49 < fenn> the zeptoliter thing assumes that your fluorescent molecule is on a 2d surface
19:49 < kanzure> instead of testing embryos why not test a cell line that produces spermatogonia and eggs and stuff
19:49 < fenn> well sure, that's the easy way
19:50 < fenn> i thought you were against that because it's more technology development
19:50 < kanzure> yashgaroth's proposal for sperm modification seems tobe reasonable
19:51 < fenn> now that i think about it, long fluorescent imaging exposure times could be phototoxic
19:53 < kanzure> btw tech development isn't my concern, it's bluesky research that worries me
19:53 < fenn> developing egg cell lines would simplify things on the macroscale
19:54 < kanzure> yashgaroth has a proposal for a thing to be doing
19:55 < fenn> https://extendfertility.com/our-process/the-egg-retrieval
19:57 < fenn> $5k per collection procedure
19:58 < fenn> also anesthesia
19:58 < kanzure> plus time and scheduling and (currently) disrupting a busy would-be parent's employment and time on the job
19:58 < kanzure> (most people can't take 3 weeks off work to go to the bahamas for IVF or whatever)
19:58 < fenn> chumps
19:59 < kanzure> yea
19:59 < fenn> sorry, i can't procreate, i'm too busy building up resources to care for my nonexistent young
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20:07 < kanzure> "Cloning of macaque monkeys by somatic cell nuclear transfer" http://www.cell.com/cell/abstract/S0092-8674(18)30057-6
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21:52 < kanzure> blort
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21:56 < fenn> blyat
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--- Log closed Thu Jan 25 00:00:09 2018