--- Log opened Sun Mar 04 00:00:09 2018
00:04 -!- TC [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap
00:04 -!- TC is now known as Guest66674
00:08 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 265 seconds]
01:13 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-rvzoviziwacmmzcv] has joined ##hplusroadmap
01:42 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
02:16 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 245 seconds]
02:32 -!- sachy [~sachy@78.108.102.220] has joined ##hplusroadmap
02:44 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap
03:24 -!- Gurkenglas [~Gurkengla@dslb-178-005-162-043.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap
03:40 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
03:41 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-rvzoviziwacmmzcv] has quit [Quit: Connection closed for inactivity]
05:11 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-iwcgnbqewhlinyxc] has joined ##hplusroadmap
06:02 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap
06:15 < kanzure> now i am become a patent
07:02 < archels> destroyer of...?
07:03 < kanzure> not sure
07:13 -!- juri_ [~juri@205.166.94.162] has quit [Remote host closed the connection]
07:18 < kanzure> zentradi
07:19 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds]
07:32 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
07:37 < kanzure> "jet powered volkswagen beetle" http://www.ronpatrickstuff.com/
07:55 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds]
08:14 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
08:23 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap
08:26 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 268 seconds]
08:35 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 256 seconds]
08:38 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
08:43 -!- DataPacRat [~dan@adsl-195.itcanada.com] has quit [Quit: Leaving.]
08:49 -!- yashgaroth [~yashgarot@2606:6000:c308:f700:a119:2c6a:d5e8:b75a] has joined ##hplusroadmap
08:51 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-iwcgnbqewhlinyxc] has quit [Quit: Connection closed for inactivity]
08:55 -!- mrdata_ is now known as mrdata
09:06 < kanzure> .tw https://twitter.com/waxwing__/status/970197596806533120
09:06 < yoleaux> Great lecture on sigma protocols and ZKPs: https://www.youtube.com/watch?v=nwsmG3S9wIc (@waxwing__)
09:06 < kanzure> "The evolutionary origins of hierarchy" http://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1004829&type=printable
09:12 -!- suhdood [~Thaelim@2601:4a:c101:d1de:a164:b860:6d49:6dbd] has joined ##hplusroadmap
09:25 < kanzure> .tw https://twitter.com/DARPA/status/969320144819376129
09:25 < yoleaux> Following traumatic injury, the time from event to first medical treatment is often the most significant factor in determining life or death. Our new Biostasis program aims to extend "golden hour" by slowing biological time. #biochemistry #MilitaryMedicine http://www.darpa.mil/news-events/2018-03-01 https://pbs.twimg.com/media/DXO3iGPXUAEI_L4.jpg (@DARPA)
09:27 < kanzure> .tw https://twitter.com/albertcardona/status/969061605903360000
09:27 < yoleaux> The ~1,600 neurons of one hemisphere of the #Drosophila larval brain, reconstructed from electron microscopy by the hand of many students, postdocs and even professors. The brain is so much more than its overstudied mushroom bodies (yellow, both present for reference). https://video.twimg.com/ext_tw_video/969060825502646272/pu/pl/qakJwpP-xqpjJOUD.m3u8 (@albertcardona)
09:28 < kanzure> .tw https://twitter.com/eboyden3/status/968163170970939392
09:28 < yoleaux> MIT News: announcing Archon, a new fluorescent voltage indicator for imaging neural activity. We found it by inventing a robotic method for doing directed evolution in mammalian cells. Paper to appear today in Nature Chemical Biology. http://news.mit.edu/2018/seeing-brains-electrical-activity-0226 (@eboyden3)
09:29 < kanzure> .tw https://twitter.com/tlambert03/status/968990314689126400
09:29 < yoleaux> recently released a new user-editable fluorescent protein database http://fpbase.org ... spread the word and/or let me know if you have any suggestions. more info on usage here: http://fpbase.org/about/ (@tlambert03)
09:29 < kanzure> "A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters" https://www.nature.com/articles/s41589-018-0004-9
09:32 < kanzure> "We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing proteins that simultaneously exhibit several specific properties, we can screen hundreds of thousands of proteins in a library in just a few hours, ...
09:32 < kanzure> ...evaluating each along multiple performance axes. To demonstrate the power of this approach, we created a genetically encoded fluorescent voltage indicator, simultaneously optimizing its brightness and membrane localization using our microscopy-guided cell-picking strategy. We produced the high-performance opsin-based fluorescent voltage reporter Archon1 and demonstrated its utility by imaging ...
09:32 < kanzure> ...spiking and millivolt-scale subthreshold and synaptic activity in acute mouse brain slices and in larval zebrafish in vivo. We also measured postsynaptic responses downstream of optogenetically controlled neurons in C. elegans.
09:32 < kanzure> "
09:32 < kanzure> hmm i guess you could do this without growing new cells, you just dump in more plasmids until you see fluorescence that you're interested in?
09:54 -!- Jenda_ [~jenda@coralmyn.hrach.eu] has quit [Ping timeout: 255 seconds]
10:00 -!- Jenda [~jenda@coralmyn.hrach.eu] has joined ##hplusroadmap
10:19 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-dhgfihgkyalnnvbx] has joined ##hplusroadmap
10:30 -!- juri_ [~juri@205.166.94.162] has joined ##hplusroadmap
10:31 -!- jtimon [~quassel@41.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 256 seconds]
10:34 -!- suhdood [~Thaelim@2601:4a:c101:d1de:a164:b860:6d49:6dbd] has quit [Remote host closed the connection]
10:34 -!- suhdood [~Thaelim@2601:4a:c101:d1de:a164:b860:6d49:6dbd] has joined ##hplusroadmap
10:34 -!- suhdood [~Thaelim@2601:4a:c101:d1de:a164:b860:6d49:6dbd] has quit [Max SendQ exceeded]
10:48 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds]
11:02 -!- jtimon [~quassel@41.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap
11:19 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
11:49 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 256 seconds]
12:00 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
12:09 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds]
12:15 -!- aeiousomething [~aeiousome@27.59.199.83] has joined ##hplusroadmap
12:16 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap
12:29 -!- sachy [~sachy@78.108.102.220] has quit [Quit: Leaving.]
12:39 -!- aeiousomething [~aeiousome@27.59.199.83] has quit [Ping timeout: 256 seconds]
12:41 < Solgriffin> https://www.irccloud.com/pastebin/nFiOfodc/
12:42 < Solgriffin> It's shorter then posting the whole think on the photolithography comment i made I gues
12:42 < Solgriffin> think=thing
12:44 -!- drewbot [~cinch@184.72.141.126] has quit [Remote host closed the connection]
12:58 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 252 seconds]
13:19 < nmz787> Solgriffin: e-beam direct write is a thing, and multi-beam e-beam systems have been in development for a while, for example a 64 beam system at ASML i think
14:21 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-dhgfihgkyalnnvbx] has quit [Quit: Connection closed for inactivity]
14:34 -!- aeiousomething [~aeiousome@124.123.12.253] has joined ##hplusroadmap
14:45 -!- aeiousomething [~aeiousome@124.123.12.253] has quit [Ping timeout: 256 seconds]
15:19 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap
16:17 < fenn> Solgriffin: check out http://tera-print.com and "beam pen lithography" https://www.nature.com/articles/nnano.2010.161
16:18 < fenn> highly parallel custom photolithography at nanometer scales
16:22 < fenn> huh "apertureless beam pen lithography. Experimental results reveal that significant UV energy can pass through the apex of a fully chromium-coated polyurethane acrylate pyramid"
16:24 < kanzure> kent had a scheme using dip pen for combinatorial molecular exploration things
16:26 < superkuh> Edward Hutchinson Synge do (essentially) that back in 1928 and talked about in his correspondence with Einstein.
16:26 < superkuh> Er, did.
16:28 < superkuh> Ah. He didn't do it. He just conceptualized it.
16:36 -!- ebowden_ [~ebowden@128.250.0.208] has quit [Remote host closed the connection]
16:39 -!- FourFire [~FourFire@234.103.189.109.customer.cdi.no] has joined ##hplusroadmap
17:00 -!- ebowden [~ebowden@128.250.0.208] has joined ##hplusroadmap
17:09 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Killed (verne.freenode.net (Nickname regained by services))]
17:10 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap
17:24 < kanzure> so much easier that way :)
17:33 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds]
17:54 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap
17:55 -!- ebowden [~ebowden@128.250.0.208] has quit [Remote host closed the connection]
18:10 -!- ebowden [~ebowden@128.250.0.206] has joined ##hplusroadmap
18:34 -!- DataPacRat [~dan@adsl-195.itcanada.com] has joined ##hplusroadmap
18:36 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Ping timeout: 276 seconds]
18:55 -!- DataPacRat [~dan@adsl-195.itcanada.com] has quit [Ping timeout: 245 seconds]
18:57 -!- ebowden [~ebowden@128.250.0.206] has quit [Remote host closed the connection]
19:06 -!- ebowden [~ebowden@128.250.0.206] has joined ##hplusroadmap
19:10 -!- DataPacRat [~dan@adsl-160.itcanada.com] has joined ##hplusroadmap
19:11 -!- FourFire [~FourFire@234.103.189.109.customer.cdi.no] has quit [Quit: Leaving]
19:32 -!- Avian-Anathema [~Garret@165.227.217.42] has quit [Quit: ZNC 1.7.x-git-876-42939c9 - https://znc.in]
19:33 -!- CaptHindsight [~2020@c-73-50-59-134.hsd1.il.comcast.net] has joined ##hplusroadmap
19:33 -!- CaptHindsight [~2020@c-73-50-59-134.hsd1.il.comcast.net] has quit [Changing host]
19:33 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap
19:34 -!- Avian-Anathema [~Garret@165.227.217.42] has joined ##hplusroadmap
19:34 < kanzure> CaptHindsight: let's write a proposal to iarpa
19:35 -!- MrHindsight [~2020@unaffiliated/capthindsight] has quit [Ping timeout: 256 seconds]
19:44 < jrayhawk>  /win 2
19:44 < jrayhawk> whoops
19:46 -!- CandleGlow [~CandleGlo@unaffiliated/candleglow] has quit [Quit: ...and all my responses they stay the same.]
19:58 < kanzure> yerp
19:58 < kanzure> such is life
20:13 -!- ebowden [~ebowden@128.250.0.206] has quit [Remote host closed the connection]
20:52 -!- jtimon [~quassel@41.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 245 seconds]
21:01 -!- ebowden [~ebowden@128.250.0.206] has joined ##hplusroadmap
21:17 -!- aeiousomething [~aeiousome@124.123.12.253] has joined ##hplusroadmap
21:22 -!- aeiousomething [~aeiousome@124.123.12.253] has quit [Ping timeout: 248 seconds]
21:23 -!- strages [uid11297@gateway/web/irccloud.com/x-owfllmwwjgpszyvh] has joined ##hplusroadmap
21:26 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap
21:45 -!- ebowden [~ebowden@128.250.0.206] has quit [Quit: Leaving...]
22:07 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection]
22:19 -!- yashgaroth [~yashgarot@2606:6000:c308:f700:a119:2c6a:d5e8:b75a] has quit [Quit: Leaving]
22:31 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Ping timeout: 260 seconds]
22:31 -!- CaptHindsight [~2020@38.84.134.54] has joined ##hplusroadmap
22:31 -!- CaptHindsight [~2020@38.84.134.54] has quit [Changing host]
22:31 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap
22:46 -!- augur [~augur@2600:380:4439:7f78:f01e:aa95:adec:4e17] has joined ##hplusroadmap
23:30 -!- DataPacRat [~dan@adsl-160.itcanada.com] has quit [Ping timeout: 268 seconds]
23:43 -!- Gurkenglas [~Gurkengla@dslb-178-005-162-043.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 256 seconds]
--- Log closed Mon Mar 05 00:00:10 2018