--- Log opened Tue Mar 27 00:00:31 2018
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00:35 < nmz787> http://diyhpl.us/~nmz787/pdf/Versatile_on-Stage_Microfluidic_System_for_Long_Term_Cell_Culture_Micromanipulation_and_Time_Lapse_Assays.pdf
00:35 < nmz787> "The microcapillaries used for microinjection were with an outer diameter of 0.8μm and an inner diameter of 0.5 μm, while those used for negative-pressure inspiration were with an inner diameter of 6 μm. "
00:35 < nmz787> "They accessed the culture cells at a 45° angle through the top window of the CCA system which was opened for the micromanipulation but closed after the micromanipulation."
00:35 < nmz787> " The cells were injected with GFP plasmid (83μg/L in concentration) into the nucleus, and the average injection volume was 180 fL."
00:35 < nmz787> "A square frame on the culture slide shown in Fig. 5 was sculptured by the violet laser beam of the Microlaser Combisystem to indicate the position of the selected cell."
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05:04 < adlai> kanzure: i thought what really mattered was splicing peyote genes into cubensis, and headlesschicken^H^H^Hmachinelearning a san pedro variant that produces 2C-crap
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06:49 < kanzure> "Using deep learning to model the hierarchical structure and function of a cell" https://sci-hub.tv/10.1038/nmeth.4627
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07:01 < wrldpc1> yeahhhhhhh!!
07:02 < wrldpc1> cubeote!!
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07:16 < kanzure> "technology maximalist"
07:18 < redlegion> Lol, yer terminfo is fuba^H^H^H^H broken, adlai
07:19 < adlai> ehhh, more of an action stationaricist
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07:48 < kanzure> "BranchScope: A new side-channel attack on directional branch predictor" http://www.cs.ucr.edu/~nael/pubs/asplos18.pdf
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08:14 < kanzure> .title https://www.greaterwrong.com/posts/7H6QbvpJgj75Ex3FJ/preprint-for-commenting-digital-immortality-theory-and
08:14 < yoleaux> [Preprint for commenting] Digital Immortality: Theory and Protocol for Indirect Mind Uploading - LessWrong 2 viewer
08:14 < kanzure> yeah don't reconstruct me from my text output... reconstruct me from my brain tissue.
08:23 < adlai> reconstruction from text output is how you kill jazz musicians. please don't kill jazz musicians.
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09:23 < kanzure> https://venturebeat.com/2018/03/27/opentrons-raises-10-million-for-scientists-to-automate-lab-experiments-with-robotics/
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10:53 < CaptHindsight> not bad for some t-slot and some *duinos
10:54 < CaptHindsight> could just use LCNC and Freecad
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10:58 < kanzure> CaptHindsight: i think they sell hardware tho, which freecad doesn't do
11:00 < CaptHindsight> kanzure: the point was that they didn't have to write a programming tool or motion controller from scratch
11:00 < CaptHindsight> but somebody must think that their python thingy is worth some $$
11:01 < CaptHindsight> nice sales job
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12:26 < kanzure> https://www.wired.com/story/live-forever-synthetic-human-genome/
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13:41 < kanzure> "Could failure in preimplantation genetic diagnosis justify editing the human embryo genome?" http://www.cell.com/cell-stem-cell/fulltext/S1934-5909(18)30004-3
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16:25 < superkuh> lytro (light field camera) went out of business.
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17:06 < juri_> branchscope is no fun.
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17:48 < nmz787> CaptHindsight: you can't really get too far brushing off opentrons when you compare to the competition which is probably 10-50X more expensive
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18:35 < l_wl> Howdy y'all, I'm trying to get a research paper in the headphone industry space but I can't get it through sci-hub or using any of my friends college logins. So looking for help here <3
18:35 < fenn> go away
18:35 < l_wl> (Nor lib-get) I just need any of these four reports \/\/
18:35 < l_wl> https://www.grandviewresearch.com/industry-analysis/earphone-and-headphone-market
18:35 < l_wl> https://www.arizton.com/market-reports/headphones-market-report
18:35 < l_wl> https://www.technavio.com/report/global-consumer-electronics-global-headphone-market-2017-2021
18:35 < l_wl> https://www.gminsights.com/industry-analysis/earphone-and-headphone-market
18:35 < fenn> these are not academic papers
18:35 < fenn> you will never find anyone who will share these reports
18:36 < fenn> also, they're probably useless in the sense that you can get the same info by walking around best buy and asking people what they want
18:36 < l_wl> No their not. But they're needed because I'm applying for grants in the hard of hearing audio space
18:37 < l_wl> I'm developing the next type of bone-conduction transducers, eg: magneto restrictive transducers
18:37 < fenn> you don't need a $10k market research report to get a grant
18:38 < l_wl> Normally you don't, except when they've asked for detailed reports on the industry and why they think we're competiive
18:39 < l_wl> And the ones we sent in were apparently not "official" or all encompassing enough
18:39 < l_wl> fenn: want to pm?
18:39 < fenn> no, i just decided i don't care
18:39 < fenn> good luck with your grant
18:39 < l_wl> fenn: ok thanks, np :)
18:40 < kanzure> on your second link their shopping cart is vulnerable to a basic attack that you could use to get the report for free
18:40 < kanzure> ... if it's an automated system.
18:41 < l_wl> kanzure: cool, I'll check that out (I aint no pro at this but will get back to you re/ it)
18:41 < kanzure> for $10,000 i could send you a report about how to do this.
18:41 < l_wl> lewls
18:46 < nmz787> lol
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19:20 < CaptHindsight> nmz787: not sure about the new software, they could have just used LCNC and Freecad and it would be more intuitive
19:24 < CaptHindsight> nmz787: others have said it wastes to much plastic, uses hand operated pipettes, wastes too much motion etc etc
19:24 < CaptHindsight> but better than nothing
19:26 < CaptHindsight> https://opentrons.com/pipettes  might be newer versions
19:26 < nmz787> CaptHindsight: I have only heard "you program it through Python" and that same person saying "here are my scripts if you want them"
19:26 < nmz787> I like Python
19:27 < nmz787> I mean, I only have manual pipettes as it is...
19:27 < nmz787> so a robot that accepts that sounds pretty darn simple of an upgrade
19:27 < nmz787> wasting time means nothing, I am too lazy to go into the next room to manually pipette
19:27 < nmz787> so it will be faster than me
19:29 < CaptHindsight> I thought biologists only program with dirt, blood and slimy stuff
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19:35 < kanzure> "Synthetic DNA synthesis and assembly: Putting the synthetic in synthetic biology" http://diyhpl.us/~bryan/papers2/DNA/Synthetic%20DNA%20synthesis%20and%20assembly:%20Putting%20the%20synthetic%20in%20synthetic%20biology%20-%202017.pdf
19:40 < nmz787> kanzure: did you highlight that stuff?
19:46 < kanzure> probably bill efcavitch
19:46 < kanzure> not me
19:48 < nmz787> "In conclusion, if the EDS data was not acquired by a well-trained SEM-EDS user, the result should not be trusted with high degree of confidence.
19:48 < nmz787> "
19:48 < nmz787> "Under the typical SEM conditions, the electron beam will induce localized charging on the scanned region of interest. Charging is a big problem and causes unpredictable data in the EDS spectrum."
19:56 < l_wl> nmz787: y'all aint involved in the SEM proj to read off data r you?
19:57 < kanzure> we only write data to DNA molecules using biology, no SEM required
19:58 < l_wl> Okk, gl with the read and write times
19:59 < kanzure> "just use parallelization!"
20:01 < juri_> more processors!
20:01 < kanzure> also we have a method of even faster writes without enzymes and without SEMs but it does require nanopores or something
20:01 < nmz787> l_wl: no I haven't read any DNA with my SEM yet... there were other folks who got OK results with a TEM, but I am looking more at quantum-tunneling in solid-state nanopores
20:02 < juri_> nmz787: o/
20:03 < nmz787> DNA+heavy metal stain == OK contrast in TEM... sample prep requires the DNA to be nicely linearized, which can be troublesome but achievable in high-speed with some expertise apparently
20:06 < CaptHindsight> nanofunnel
20:07 < CaptHindsight> couple one end to a gold particle
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20:14 < nmz787> wow, the MOSIS user mailing list hasn't been posted on since 2015 :(
20:14 < nmz787> let's see if anyone's listening
20:22 < CaptHindsight> nmz787: didn't know that you went to RIT
20:23 < CaptHindsight> used to deal with Kodak
20:23 < CaptHindsight> sad to see that part of town decay
20:31 < nmz787> yeah it was pretty post-apocalyptic by the time I got there
20:31 < nmz787> which was late 2007
20:32 < nmz787> fenn (or others) do you know of any stereo microphone recorders that would be useful for sound-source triangulation?
20:32 < nmz787> it would be nice if my smartphone had this... there's an owl outside for weeks and it would be interesting to find it and, idk, photograph it I guess
20:33 < CaptHindsight> I was there last in 2013
20:34 < CaptHindsight> startups using the old remains
20:36 < nmz787> if some non-flat land was a little closer, I wouldn't mind moving back there maybe, someday
20:36 < nmz787> apparently there's no student fab in Portland
20:36 < nmz787> so that's definitely something I miss from RIT
20:37 < nmz787> I guess all the local tech infrastructure has made people around here microscope and logic nerds
20:38 < nmz787> more of the equipment side really, than using the equipment
20:45 < fenn> nmz787: i would just use a 5-7 channel usb sound card
20:47 < fenn> all the easy triangulation algorithms require impulsive sound like for example a gunshot
20:48 < fenn> you could leave out some mice and a webcam for the owl
20:50 < nmz787> hmm, you mean I can't just match the 'hoot' on each channel and compare the phase-difference?
20:53  * fenn grumbles
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20:57 < l_wl> http://sci-hub.la/https://asa.scitation.org/doi/full/10.1121/1.2184988
21:00 < l_wl> I've worked a bit on the audio side, with a few ppl. N can help on that side if any of y'all interested. But atm I'm still tryna figure out how to do this basic exploit
21:01 < l_wl> https://www.amarhelloween.com/2015/06/how-to-hack-vp-asp-shopping-websites.html for https://www.arizton.com/market-reports/akt-inhibitors-pipeline - am I on the right path here?
21:01 < kanzure> maaku wants you to build a cheap 24/7 personal audio recording device that can pipe audio to google/amazon/ibm speech api
21:02 < l_wl> ok
21:02 < kanzure> you'll get one hint: it looks like the form uses a hidden element to submit the report price. they don't seem to be validating it against their database or catalog of prices.
21:02 < l_wl> Ive played a bit with Watson thats about it
21:03 < l_wl> tyty
21:05 < nmz787> l_wl: re that article, the DAQ they used seems to be "The 6250 can be made to behave like a scope in certain conditions. It has more vertical resolution (16 bits versus 8) but a slower sampling rate. You cannot do true simultaneous acquisition but you do have 16 inputs"
21:05 < nmz787> so the non global/simulataneous recording/sampling would add some noise I think
21:06 < fenn> 8 channel simultaneous input audio cards are common
21:07 < fenn> ebay is failing me today
21:07 < l_wl>          nmz787 kk let me focus on kanzure's suggestions
21:07 < l_wl> brb
21:07 < l_wl> <3
21:10 < nmz787> fenn: seems like it's already sounding easier to try cobbling something together with an arduino or something https://www.adafruit.com/product/2716
21:10 < nmz787> those MEMS mics are $5
21:10 < fenn> ok
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21:11 < nmz787> I could be super lazy and get two arduinos to record each a single mic to an SD card, and then simply trigger recording with a single switch
21:11 < fenn> physically small mics have poor low frequency response
21:11 < nmz787> to ensure they're synced
21:11 < nmz787> ah
21:11 < nmz787> hm
21:11 < nmz787> I can hear this owl inside my house now, with the TV on
21:12 < nmz787> but I guess I can try recording with my phone, and seeing if I can hear it in the recording
21:13 < nmz787> hmm, combine this with google-cardboard and pay someone to develop a VR app to show stuff?
21:13 < nmz787> "hooter vision
21:13 < nmz787> "
21:31 < CaptHindsight> I have barking dog next door
21:31 < CaptHindsight> I know exactly where and when he is there
21:32 < CaptHindsight> still annoys us  :)
21:32 < nmz787> this owl is more of a calming sort of joke-able thing for me
21:33 < nmz787> I've heard it during the day, all hours of the night (that I've been awake to simply notice)
21:34 < nmz787> but I guess I don't know if there are multiple, or just one owl
21:35 < nmz787> if it's only one, what's it doing, trying to attract a mate?
21:35 < nmz787> fending off territory?
21:35 < nmz787> is it the animal kingdom equivalent of a blinking red light in amsterdam?
21:36 < nmz787> hooting alllll-nigghttt-lonngggg
21:36 < l_wl> last
21:50 < CaptHindsight> nmz787: any bears in the area?
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22:18 < nmz787> not that I've heard of around here specifically, but a few years ago this happened http://www.kptv.com/story/25749169/bear-on-the-loose-in-ne-portland
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22:25 < CaptHindsight> was thinking bear bait up in that tree
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--- Log closed Wed Mar 28 00:00:31 2018