--- Log opened Thu Jan 03 00:00:10 2019 03:40 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Remote host closed the connection] 05:46 -!- l_wl [~l_wl@pool-173-66-183-216.washdc.fios.verizon.net] has joined ##hplusroadmap 06:20 -!- aeiousomething [~aeiousome@unaffiliated/aeiousomething] has joined ##hplusroadmap 07:15 -!- sachy [~sachy@91.146.121.5] has joined ##hplusroadmap 08:01 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 08:42 -!- strages [uid11297@gateway/web/irccloud.com/x-dwosibfelhnfmtbe] has joined ##hplusroadmap 09:40 -!- yashgaroth [~yashgarot@cpe-66-27-127-185.san.res.rr.com] has joined ##hplusroadmap 11:22 < archels> "another evening wasted" https://chakazul.github.io/Lenia/JavaScript/Lenia.html 12:13 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 246 seconds] 13:50 < nsh> nice 13:55 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 245 seconds] 14:22 -!- sachy [~sachy@91.146.121.5] has quit [Quit: Leaving.] 15:10 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 16:18 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [] 16:23 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 16:49 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 246 seconds] 16:59 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 17:27 < kanzure> http://www.the-odin.com/biohacker-101-class/ 17:34 -!- Jay_Dugger [~jwdugger@47.185.249.138] has joined ##hplusroadmap 17:38 -!- Jay_Dugger [~jwdugger@47.185.249.138] has quit [Client Quit] 18:07 -!- balrog [~balrog@unaffiliated/balrog] has quit [Quit: Bye] 18:12 -!- balrog [~balrog@unaffiliated/balrog] has joined ##hplusroadmap 18:23 -!- strages [uid11297@gateway/web/irccloud.com/x-dwosibfelhnfmtbe] has quit [Quit: Connection closed for inactivity] 19:00 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] 19:03 < kanzure> "Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas" https://www.biorxiv.org/content/early/2018/12/27/507293#disqus_thread 19:04 < kanzure> "A dendritic subtrate for the cholinergic control of neocortical output neurons" https://www.cell.com/neuron/fulltext/S0896-6273(18)31044-4 19:04 < kanzure> "Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER" https://www.biorxiv.org/content/early/2018/12/28/507566 https://twitter.com/eboyden3/status/1079716347167342592 19:05 < kanzure> .tw 19:05 < yoleaux> From Peng Yin's group at Harvard: how to see many kinds of protein in a biological specimen, one after the other, using DNA-barcoded antibodies that can be equipped with glowing dyes. Speeds up cycles of protein staining for expansion microscopy by 10x: https://www.biorxiv.org/content/early/2018/12/28/507566 https://pbs.twimg.com/media/DvvsPt3XcAAkI97.jpg (@eboyden3) 19:06 < kanzure> .tw https://twitter.com/balajis/status/1078945036723617792 19:06 < yoleaux> Brain-computer interfaces are my candidate for most underhyped technology relative to progress. They are far ahead of general public perception. People know that drones or self-driving cars are happening, but don’t realize how far BCI has gotten. https://twitter.com/TheEconomist/status/1078937242968735744 (@balajis) 19:06 < kanzure> .tw https://twitter.com/AdamMarblestone/status/1078394454283825152 19:06 < yoleaux> I love this idea of a sun-shade at the L1 Lagrange point http://www.webpages.uidaho.edu/envs501/downloads/Geoengineering%20SCIAM%202008.pdf https://www.pnas.org/content/103/46/17184 But how to offset the cost? Mine BTC out at L1 w/ massive solar power and cooling? Seems insane, but then you see things like https://www.planetaryresources.com/2018/10/consensys-acquires-planetary-resources/ and think "what if..." (@AdamMarblestone) 19:07 < kanzure> "Recurrence-based information processing in gene regulatory networks" https://www.ncbi.nlm.nih.gov/pubmed/30384649 19:07 < kanzure> "Calculating coherent light-wave propagation in large heterogeneous media" https://arxiv.org/abs/1812.10463 https://twitter.com/j_bertolotti/status/1078244617534033920 19:07 < kanzure> "Walking along chromosomes with super-resolution imaging, contact maps, and integrative modeling" https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007872 19:08 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 19:09 < kanzure> "A 3.8 angstrom cryo-EM structure of a small protein bound to a modular imaging scaffold" https://www.biorxiv.org/content/early/2018/12/24/505792 https://twitter.com/srikosuri/status/1077303323831689216 19:52 -!- cluelessperson [~cluelessp@unaffiliated/cluelessperson] has quit [Ping timeout: 252 seconds] 19:57 -!- cluelessperson [~cluelessp@unaffiliated/cluelessperson] has joined ##hplusroadmap 21:08 -!- aeiousomething [~aeiousome@unaffiliated/aeiousomething] has quit [Ping timeout: 250 seconds] 21:14 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 21:17 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 245 seconds] 21:22 -!- yashgaroth [~yashgarot@cpe-66-27-127-185.san.res.rr.com] has quit [Quit: Leaving] 23:48 < nmz787> spin-coated some PMMA tonight and exposed with FIB, developed with 4:1 ethanol:water... surprisingly easy aside from getting streak-free films and focusing the FIB (it's like a timed video game, since you expose/obliterate your material as you're working on focus!). 23:55 < fenn> so your FIB is working? 23:55 < nmz787> I did this at the shop I work part-time at 23:56 < nmz787> getting ansy about setting up my FIB though 23:56 < nmz787> was planning on clearing space in the garage over the past week and a half since I've been off of day-job work... but been parenting too much 23:56 < nmz787> www.msg.ucsf.edu/BI204/Lectures/Q2/Cheng/day2_1995_QRB_Henderson.pdf "The potential and limitations of neutrons,electrons and X-rays for atomic resolutionmicroscopy of unstained biological molecules" 23:57 < nmz787> that's ref 6 from the cryo-EM paper kanzure just posted 23:57 < nmz787> I am still not clear on why there's a 38kDa lower limit --- Log closed Fri Jan 04 00:00:11 2019