--- Log opened Mon Feb 04 00:00:40 2019 00:18 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has joined ##hplusroadmap 00:56 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 246 seconds] 01:06 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 01:24 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 01:28 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 240 seconds] 02:41 -!- Pompolic_ [~A@unaffiliated/pompolic] has joined ##hplusroadmap 02:43 -!- pompolic [~A@unaffiliated/pompolic] has quit [Ping timeout: 246 seconds] 02:44 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 246 seconds] 03:27 -!- Pompolic_ is now known as pompolic 04:44 -!- jtimon [~quassel@92.28.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 04:59 -!- ninja_ [2f583a89@gateway/web/freenode/ip.47.88.58.137] has joined ##hplusroadmap 07:53 -!- mrdata| is now known as mrdata- 07:58 -!- ninja_ [2f583a89@gateway/web/freenode/ip.47.88.58.137] has quit [Ping timeout: 256 seconds] 08:43 -!- strages [uid11297@gateway/web/irccloud.com/x-pvcxuteunvvcaqtk] has joined ##hplusroadmap 09:16 -!- yashgaroth [~yashgarot@cpe-66-27-127-185.san.res.rr.com] has joined ##hplusroadmap 11:27 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 11:45 -!- kursk [~kursk@101.37-191-143.fiber.lynet.no] has quit [Remote host closed the connection] 11:51 -!- Urchin [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 12:31 -!- Livestradamus [~Livestrad@unaffiliated/livestradamus] has quit [Quit: WeeChat 2.2] 12:34 -!- Livestradamus [~Livestrad@unaffiliated/livestradamus] has joined ##hplusroadmap 12:48 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 250 seconds] 14:52 -!- Urchin [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 15:03 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has quit [Ping timeout: 240 seconds] 16:09 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 246 seconds] 16:12 -!- jtimon [~quassel@92.28.134.37.dynamic.jazztel.es] has quit [Quit: gone] 16:23 -!- jtimon [~quassel@92.28.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 17:42 -!- balrog [~balrog@unaffiliated/balrog] has quit [Quit: Bye] 18:07 -!- balrog [~balrog@unaffiliated/balrog] has joined ##hplusroadmap 18:15 < justanotheruser> what is the best way to sequence dna? this looks interesting https://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/ 19:18 < jrayhawk> that depends on what you want it for 19:18 < jrayhawk> there are different levels of expense for different levels of accuracy and coverage 19:41 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 20:15 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 246 seconds] 20:43 -!- strages [uid11297@gateway/web/irccloud.com/x-pvcxuteunvvcaqtk] has quit [Quit: Connection closed for inactivity] 21:05 < justanotheruser> jrayhawk: 1) self-testing DNA 2) knowledge about sequencing DNA 21:18 -!- dyce[m] [dycematrix@gateway/shell/matrix.org/x-vrudlakywumwhhne] has joined ##hplusroadmap 22:02 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=38d88ad5 Bryan Bishop: transcript: threshold signatures >> http://diyhpl.us/diyhpluswiki/transcripts/sf-bitcoin-meetup/2019-02-04-threshold-signatures-and-accountability/ 22:04 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=40341f0a Bryan Bishop: tweeter link >> http://diyhpl.us/diyhpluswiki/transcripts/sf-bitcoin-meetup/2019-02-04-threshold-signatures-and-accountability/ 22:06 -!- jtimon [~quassel@92.28.134.37.dynamic.jazztel.es] has quit [Ping timeout: 245 seconds] 22:16 -!- yashgaroth [~yashgarot@cpe-66-27-127-185.san.res.rr.com] has quit [Quit: Leaving] 22:41 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 22:47 < nmz787> justanotheruser: most likely using a PCR thermocycler as a lead-in to sequencing is targeting small chunks (few hundred basepairs), particularly via a process called 22:47 < nmz787> .wik sanger sequencing 22:47 < yoleaux> "Sanger sequencing is a method of DNA sequencing first commercialized by Applied Biosystems, based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Developed by Frederick Sanger and colleagues in 1977, it was the most widely used sequencing method for approximately 40 years." — https://en.wikipedia.org/wiki/Sanger_sequencing 23:12 -!- mrdata- [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 240 seconds] 23:17 -!- ebowden_ [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds] 23:18 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap --- Log closed Tue Feb 05 00:00:40 2019