--- Log opened Wed May 29 00:00:26 2019 01:06 < nmz787_> kanzure: like pipette pumps, or just the pipette tips? I can def get some tips, dimensions would be good to know, unless you can design a system around the tips' dimensions... but the end-diameter of the tip would def need to be known... though I could probably just get a few and send them out for you/others to check up on. How many would you want to start? 2, 5, 10? 01:40 -!- cerulean_syzygy [~cerulean_@145.37.129.53] has joined ##hplusroadmap 01:46 -!- adamsky [~adamsky3@178235181168.unknown.vectranet.pl] has joined ##hplusroadmap 01:49 -!- LeoTal [~Adium@233.170.86.79.rev.sfr.net] has joined ##hplusroadmap 02:18 -!- cerulean_syzygy [~cerulean_@145.37.129.53] has quit [Ping timeout: 268 seconds] 02:26 -!- cerulean_syzygy [~cerulean_@145.37.129.53] has joined ##hplusroadmap 02:43 -!- cerulean_syzygy [~cerulean_@145.37.129.53] has quit [Ping timeout: 248 seconds] 02:44 -!- adamsky [~adamsky3@178235181168.unknown.vectranet.pl] has quit [Remote host closed the connection] 02:44 -!- adamsky [~adamsky3@178235181168.unknown.vectranet.pl] has joined ##hplusroadmap 02:57 -!- cerulean_syzygy [~cerulean_@145.37.129.53] has joined ##hplusroadmap 03:05 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 03:10 -!- cerulean_syzygy [~cerulean_@145.37.129.53] has quit [Ping timeout: 244 seconds] 03:27 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-xtrirlsydvpecjwz] has joined ##hplusroadmap 03:45 -!- TC [~talinck@98.29.27.253] has joined ##hplusroadmap 03:49 -!- hehelleshin [~talinck@98.29.27.253] has quit [Ping timeout: 248 seconds] 05:37 -!- jtimon [~quassel@181.61.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 06:15 -!- purpleshift [purpleshif@gateway/vpn/mullvad/purpleshift] has joined ##hplusroadmap 06:41 < hrofu> Support would have to be more prevalent where I reside in order to make sense inserting a microchip in my body, but I feel like it's inevitable at this point 06:49 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 06:54 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 07:22 -!- adamsky [~adamsky3@178235181168.unknown.vectranet.pl] has quit [Remote host closed the connection] 07:40 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has joined ##hplusroadmap 07:46 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Remote host closed the connection] 07:53 -!- strages [uid11297@gateway/web/irccloud.com/x-vdjucbsgvlojwsib] has joined ##hplusroadmap 08:00 -!- uniera [~uniera@195.206.107.250] has joined ##hplusroadmap 08:22 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 08:26 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 08:46 < kanzure> nmz787: i need the micron-sized glass micropipette tips for a micromanipulator. actual micro in "micropipette". 15 micron at the end, bent at 30 degrees by a microforge. 08:53 -!- uniera [~uniera@195.206.107.250] has quit [Remote host closed the connection] 08:57 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 09:05 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 09:06 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Remote host closed the connection] 09:07 -!- spaceangel 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is now known as Jenda` 11:01 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 11:06 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Ping timeout: 248 seconds] 11:32 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 11:37 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 11:42 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 11:44 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 11:56 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 12:00 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Ping timeout: 246 seconds] 12:07 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 12:20 < fenn> the chip in my credit card is broken after a couple years already, i'd hate to have to get it dug out from under my skin just to get an update/replacement 12:21 < fenn> security flaws are found all the time 12:59 -!- justanotheruser is now known as Mr_Notheruser 13:17 -!- Mr_Notheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 13:18 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 13:27 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 13:41 < nmz787_> kanzure: bent near the shank where the manipulator would hold on, or bent near the tip? Also micron sized at the tip end seems too big for microinjection... but maybe I'm wrong. I seem to recall some stuff uses two sizes, one to hold a cell in place, and another to actually inject 13:41 < nmz787_> kanzure: also the bending 30 degrees might not be possible on my end... but it should just be a matter of torching the pipette tips until they droop enough 13:41 < nmz787_> (I think) 13:47 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 13:49 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 14:03 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Remote host closed the connection] 14:07 -!- uniera [~uniera@bl6-214-176.dsl.telepac.pt] has joined ##hplusroadmap 14:07 -!- uniera_ [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 14:08 -!- NikopolSohru [~Nikopol_@93.190.137.186] has joined ##hplusroadmap 14:08 -!- NikopolSohru [~Nikopol_@93.190.137.186] has quit [Changing host] 14:08 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has joined ##hplusroadmap 14:10 -!- sachy [~sachy@91.146.121.5] has quit [Quit: Leaving.] 14:12 -!- uniera [~uniera@bl6-214-176.dsl.telepac.pt] has quit [Ping timeout: 272 seconds] 14:13 -!- uniera_ [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Remote host closed the connection] 14:13 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 14:58 -!- LeoTal [~Adium@233.170.86.79.rev.sfr.net] has quit [Quit: Leaving.] 15:04 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Ping timeout: 248 seconds] 15:05 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 15:24 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has quit [Remote host closed the connection] 15:46 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Read error: Connection reset by peer] 16:10 -!- Hooloovo0 [Hooloovoo@sorunome.de] has quit [Remote host closed the connection] 16:13 -!- Hooloovo0 [Hooloovoo@sorunome.de] has joined ##hplusroadmap 16:18 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has quit [Quit: Leaving] 16:25 -!- purpleshift [purpleshif@gateway/vpn/mullvad/purpleshift] has quit [Quit: .] 16:26 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-xtrirlsydvpecjwz] has quit [Quit: Connection closed for inactivity] 16:34 < yashgaroth> nmz787_ this covers the basic techniques required http://news.narishige-group.com/pdf/news029en.pdf 17:30 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has quit [Read error: Connection reset by peer] 17:33 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has joined ##hplusroadmap 17:36 -!- periffis_ [~g@66.205.193.237] has joined ##hplusroadmap 17:36 -!- periffis_ [~g@66.205.193.237] has quit [Client Quit] 17:38 -!- periffis [~g@66.205.193.237] has quit [Ping timeout: 248 seconds] 17:50 -!- jtimon [~quassel@181.61.134.37.dynamic.jazztel.es] has quit [Quit: gone] 18:13 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 18:16 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has quit [Ping timeout: 246 seconds] 18:18 -!- strages [uid11297@gateway/web/irccloud.com/x-vdjucbsgvlojwsib] has quit [Quit: Connection closed for inactivity] 18:21 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 18:22 -!- periffis [~g@66.205.193.237] has joined ##hplusroadmap 18:24 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 272 seconds] 18:26 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Quit: Leaving...] 18:58 -!- yashgaroth [~ffffffff@2606:6000:ca81:3100:cc6b:4bb6:15b9:d343] has joined ##hplusroadmap 19:41 -!- periffis [~g@66.205.193.237] has quit [Quit: periffis] 20:09 -!- justan0theruser is now known as justanotheruser 20:54 -!- yashgaroth [~ffffffff@2606:6000:ca81:3100:cc6b:4bb6:15b9:d343] has quit [Read error: Connection reset by peer] 20:55 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has joined ##hplusroadmap 21:00 -!- periffis [~g@66.205.193.237] has joined ##hplusroadmap 21:58 -!- juri_ [~juri@205.166.94.162] has quit [Ping timeout: 245 seconds] 22:00 < nmz787_> yashgaroth: I'm seeing $3 each for 0.3 micron tip pipette tips 22:00 < nmz787_> have you seen better/worse pricing? 22:00 < nmz787_> they aren't angled though 22:00 < nmz787_> err, sorry, $10 each 22:01 < nmz787_> https://fivephoton.com/index.php?route=product/product&product_id=189 22:02 < nmz787_> here are raw capillary prices https://www.sutter.com/MICROPIPETTE/glass.html 22:02 < nmz787_> I've got access to a sutter instruments puller 22:02 < nmz787_> but I'm mainly familiar with using the FIB to cut the tips 22:03 < nmz787_> (to size) 22:03 < yashgaroth> yeah they still need to get angled in a microforge, or whatever'ed to become a holding pipette 22:06 < yashgaroth> might be able to get the angling done without a forge, but apparently you need the pipette pointing downward during heating and the hot air pulls/bends the pipette upward 22:11 < yashgaroth> may also need them more cylindrical at the pointy end, so we've got a single column of cells loaded in it, which should be a parameter on the pipette puller 22:17 < yashgaroth> but yeah for the price of 100 pre-pulled we could pick up a used puller and some raw capillaries, anyway we can dig into it tomorrow 22:17 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has quit [Quit: Leaving] --- Log closed Thu May 30 00:00:26 2019