--- Log opened Mon Oct 21 00:00:40 2019
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03:30 < nmz787> https://pubs.acs.org/doi/10.1021/acssynbio.9b00243
03:30 < nmz787> .title
03:31 < nmz787> "Off-colony screening of biosynthetic libraries by rapid laser-enabled mass spectrometry"
03:31 < nmz787> "we demonstrate the use of automated Laser-Assisted Rapid Evaporative Ionisation Mass Spectrometry (LA-REIMS) - a form of ambient laser desorption ionisation mass spectrometry - to perform rapid mass spectrometry analysis direct from agar plate yeast colonies without sample preparation or extraction. We use LA-REIMS to assess production levels of violacein and betulinic acid directly from yeast
03:31 < nmz787> colonies at a rate of 6 colonies per minute. We then demonstrate the throughput enabled by LA-REIMS by screening over 450 yeast colonies in under 4 hours, while simultaneously generating recoverable glycerol stocks of each colony in real-time."
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07:35 < docl> http://triggerwarning.us/defying-ageing-with-ariel-va-feinerman-a-radical-exploration/
07:40 < kanzure> jrayhawk: maaaybe the first episode. i think i'm in the first or second episode. for like 2 seconds.
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07:51 < U_U> I grew some yeast last week
07:52 < U_U> - it really went well, I'm using it for methionine restricted diet
07:52 < U_U> - ie. fermenting the amino source ;)
07:54 < U_U> - technically its actually Lactobacillus, Candida and some other microbes
07:58 < U_U> +Sccharomyces
08:02 < U_U> actually, "Candida" is not correct, thats old info, and its Sachharomyces, and
08:03 < U_U> hm, actually, it may be Candida milleri is in ther as well
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10:13 < superkuh> TIL small population neuron types like dopaminergic, cholinergic, serotonergic, etc have single-cell aggregate lengths of hundreds of meters.
10:13 < superkuh> https://luysii.wordpress.com/2019/10/20/why-dont-serotonin-neurons-die-like-dopamine-neurons-do-in-parkinsons-disease/
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10:30 < Fr0D17> Anyone had experience with vyvanse? Specifically on and off use.
10:31 < kanzure> take lots
10:32 < kanzure> problem solved
10:32 < Fr0D17> side effects? Trying to stay away from those.
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11:10 < U_U> well, none of the people I know that have ADHD meds have fared very well
11:10 < U_U> Fr0D17: ^
11:13 < Fr0D17> I was originally on Nuvigil for "fatigue
11:13 < Fr0D17> new doctor for some reason really wanted me on vyvanse but im in my mid 30s and have never had an ADHD problem.
11:16 < Fr0D17> This is my fifth day off of vyvanse and I feel tons better. Im going to discontinue it for good. Thanks for the input.
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11:19 < U_U> well
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11:54 < docl> nuvigil = armodafinil
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12:49 < kanzure> "Search-and-replace genome editing without double-strand breaks or donor DNA" https://www.nature.com/articles/s41586-019-1711-4
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14:17 < nmz787> .title https://www.nature.com/articles/s41586-019-1711-4
14:17 < EmmyNoether> Search-and-replace genome editing without double-strand breaks or donor DNA | Nature
14:17 < nmz787> disabled Cas9 fused with Reverse Transcriptase
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16:25 < kanzure> hmph
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--- Log closed Tue Oct 22 00:00:41 2019