--- Log opened Thu Mar 12 00:00:23 2020 00:19 -!- Human_G33k [~HumanG33k@62.147.242.8] has quit [Quit: Leaving] 01:43 -!- darsie [~kvirc@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 01:53 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 01:54 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 258 seconds] 02:26 < nmz787> .title https://imgur.com/a/f2y7OVO 02:26 < saxo> vacuum deposition controller and hardware - Album on Imgur 02:30 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-tamqdbynwpsgvlzl] has quit [Quit: Connection closed for inactivity] 02:42 -!- darsie [~kvirc@84-114-73-160.cable.dynamic.surfer.at] has quit [Remote host closed the connection] 02:42 -!- darsie [~kvirc@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 03:00 -!- mauz555 [~mauz555@2a01:e0a:56d:9090:9d56:3455:9ea7:65f7] has joined ##hplusroadmap 03:01 -!- mauz555 [~mauz555@2a01:e0a:56d:9090:9d56:3455:9ea7:65f7] has quit [Read error: Connection reset by peer] 04:09 -!- CRM114 [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 04:12 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 258 seconds] 04:17 -!- mauz555 [~mauz555@2a01:e0a:56d:9090:9d56:3455:9ea7:65f7] has joined ##hplusroadmap 04:33 -!- HumanG33k [~HumanG33k@62.147.242.8] has joined ##hplusroadmap 04:34 -!- HumanG33k [~HumanG33k@62.147.242.8] has quit [Remote host closed the connection] 04:34 -!- HumanG33k [~HumanG33k@62.147.242.8] has joined ##hplusroadmap 04:36 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has joined ##hplusroadmap 04:54 -!- mauz555 [~mauz555@2a01:e0a:56d:9090:9d56:3455:9ea7:65f7] has quit [] 05:13 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has quit [Quit: My MacBook has gone to sleep. ZZZzzz…] 05:40 < kanzure> hm 05:48 -!- CRM114 [~urchin@unaffiliated/urchin] has quit [Ping timeout: 265 seconds] 07:14 -!- TC [~talinck@98.29.27.253] has joined ##hplusroadmap 07:15 -!- TC is now known as Guest49864 07:18 -!- hehelleshin [~talinck@98.29.27.253] has quit [Ping timeout: 265 seconds] 07:51 -!- orange_ [~textual@92.223.89.135] has joined ##hplusroadmap 08:06 -!- yashgaroth [~ffffffff@172.58.19.145] has joined ##hplusroadmap 08:23 < agile_prg> how do I get in touch with corohope? 08:36 < kanzure> no contact info in the pdf? 08:46 < agile_prg> what pdf? 08:47 < agile_prg> saxo I am in Boston and can go to boslab, the most advanced community biology lab in the world 08:47 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has joined ##hplusroadmap 08:48 < agile_prg> know this 08:49 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has quit [Client Quit] 09:15 < lsneff> Would quantum immortality guarantee that if you are cryopreserved, as long as the process doesn't destroy information, you will wake up at some point? 09:20 < lsneff> I mean, there'd always be some wave function of the universe that would include you waking up. 09:35 -!- mrdata_ is now known as mrdata 09:59 < agile_prg> I wish you would know this 10:13 < kanzure> evaluate https://www.longevity.technology/better-faster-stronger-crispr/ 10:14 -!- spaceangel [~spaceange@ip-89-177-23-46.net.upcbroadband.cz] has joined ##hplusroadmap 10:36 < yashgaroth> "we can deliver femtoliter [a unit of volume equal to 10 litres] amounts of a liquid solution into single mammalian cells" wow they can fit 10 liters into a single cell 10:37 < yashgaroth> I'm guessing the exponent got deleted, but anyway you can already inject tiny volumes into single cells with a microinjector, that's how people do regular CRISPR microinjection 10:56 < lsneff> Annoying that no one checks over their writing before publishing it. 11:14 -!- orange_ [~textual@92.223.89.135] has quit [Quit: My MacBook has gone to sleep. ZZZzzz…] 11:23 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has joined ##hplusroadmap 11:25 < dr-orlovsky> Hi all, had a concept with colleagues for COVID19 vaccine/treatment but a lab is needed able to perform experiments with virus on chimpanzee or humanized mice. Does any of you know such labs, in not-that-regulated areas of the world? 11:28 < dr-orlovsky> A concept is the following: si-RNA (small interfering RNAs) for both ACE and ACE2 in combination with angiotensin 1-7 and 1-9 olygopeptides in the form of inhalation. Should be efficient at both prevention and treatment; much cheaper to sythethise and produce in large quantities than any antibodies 11:29 < dr-orlovsky> We can provide the necessary siRNAs and olygopeptides; needs animals and lab where they can be infected and the efficiency of the treatment can be checked 11:39 < dr-orlovsky> The background: ACE2 is one the enzyme (angiotensin-converting enzyme 2) which are the part of angiotensis-renin system, involved in arterial pressure management and inflamation. It is normally expressed in many tissues, including lungs, and its expression increases with age. SARS-CoV-2, which is a "coronavirus" behind COVID-19, uses ACE2 of alveolar cells as a cell surface receptor to penetrate into them and proliferate. 11:39 < dr-orlovsky> While the initial ideas were to inhibit ACE2, it happened to be inefficient (which were shown many years ago with SARS-CoV, the SARS virus, using the same mechanism). The problems were the following: (1) ACE2 is required to maintain healty performance of angiotensin-renin system, converting "toxic" angiotensin II (which causes arterial hypertension and sever inflamation/resporatory insuffiency) into anti-inflamatory 11:39 < dr-orlovsky> angiotensin 1-7 and 1-9 olygopeptides. Angiotensin II is synthethized from Angiotensin with another enzyme, ACE (without "2"). This enzyme is a normal target for anti-hypertension therapy, and its antagonists are widely used in its treatment. However, receptor antagonists are actually causing an increase of ACE expression (which is a common case for pharmacological receptor/azyme blockers). Anyway, long story short, ACE2 11:39 < dr-orlovsky> is good, but sed by virus; ACE is bad, and when we turn ACE2 off it strikes with bad effects. 11:40 < dr-orlovsky> So, what is proposed is to use short interfering RNA (which downregulate protein expression and are much better than pharmacological antagonists in this regard) against both ACE and ACE2, combined with provision of necessary olygopeptides angiotensin 1-7 and 1-9, which would not be produced internally (because of absence of ACE2) and which are important for maintaining respiratory performance 11:42 < dr-orlovsky> Me and colleagues has ready-to-go sequences for si-RNA and olygopeptides and facilities to order them from in any reasonable quantitates. The only thing that is missed are animal tests to make sure that the proposed combination works in fact 11:55 < yashgaroth> wouldn't the best first step be to test the siRNA and oligopeptide combination on healthy animals? I don't think you will find a lab capable of BSL-3 containment in "not-that-regulated areas" that are also capable of generating good data 11:56 < yashgaroth> also due to differences in half-life between siRNA and peptides, managing a dose curve between them sounds difficult; e.g. the siRNA will work for several hours or days, while the peptide will be cleared within minutes 11:57 < kanzure> dr-orlovsky: we do have some labs available but like yashgaroth says BSL3 and also humanized mice and also a willingness to engage on this particular project... etc.. is going to be particularly hard to find. 11:58 < yashgaroth> a potential issue with siRNA is that you will see compensatory overexpression of the gene after siRNA levels drop, it depends on the gene but it can be a common issue in RNA interference experiments 11:58 < yashgaroth> I do agree that pharmacological antagonists would be counterproductive against the virus, though 11:59 < dr-orlovsky> good suggestion about trying with normal animals to ensure the combination is not toxic by itself 12:00 < yashgaroth> and I don't think there are mice with humanized pulmonary systems, only immune systems; and I'm dubious that you can simply change their ACE2 gene to the human one and get normal mice that can be infected by SARS-CoV-2 12:01 < dr-orlovsky> true 12:04 < dr-orlovsky> yashgaroth: regarding oligopeptide lifetime, I had done experiments with 4- and 8-aminoacid oligopeptides in animals and they were working quite well with a single daily injection (brain liquor & abdominal injections) 12:12 < yashgaroth> it depends on the peptide, especially a peptide like angiotensin that is regulated by progressive proteolysis 12:16 -!- Urchin [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 12:17 < dr-orlovsky> it is, however 7 and 9-acid forms are final ones with now specific proteases for them available; so just a generic proteolysis like for any other peptides — or more likely they will be cleared by receptor intake (Mas) 12:17 < dr-orlovsky> *now -> no 12:18 < dr-orlovsky> anyway it will be nice to see that in experiments anyway 12:28 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=7a788986 Michael Folkson: Add Pieter Chaincode podcast transcript >> http://diyhpl.us/diyhpluswiki/transcripts/chaincode-labs/2020-01-28-pieter-wuille/ 12:28 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=1f664128 Bryan Bishop: Merge pull request #81 from michaelfolkson/add-pieter-chaincode >> http://diyhpl.us/diyhpluswiki/ 12:41 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has quit [Quit: My MacBook has gone to sleep. ZZZzzz…] 13:14 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has joined ##hplusroadmap 13:41 -!- aeiousomething [~aeiousome@unaffiliated/aeiousomething] has joined ##hplusroadmap 13:41 -!- aeiousomething [~aeiousome@unaffiliated/aeiousomething] has quit [Client Quit] 13:41 -!- aeiousomething [~aeiousome@unaffiliated/aeiousomething] has joined ##hplusroadmap 13:44 -!- aeiousomething [~aeiousome@unaffiliated/aeiousomething] has quit [Client Quit] 14:43 -!- dr-orlovsky [~dr-orlovs@xdsl-188-155-161-135.adslplus.ch] has quit [Ping timeout: 255 seconds] 15:13 -!- spaceangel [~spaceange@ip-89-177-23-46.net.upcbroadband.cz] has quit [Ping timeout: 256 seconds] 15:28 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-bkoviwysqewympxa] has joined ##hplusroadmap 15:38 < maaku> agile_prg: saxo is a bot. beep-boop. 15:54 -!- helleshin [~talinck@98.29.27.253] has joined ##hplusroadmap 15:57 -!- Guest49864 [~talinck@98.29.27.253] has quit [Ping timeout: 255 seconds] 16:10 < kanzure> https://medium.com/working-your-core/democratizing-banking-one-api-at-a-time-a1c28ee644f9 17:48 -!- dr-orlovsky [~dr-orlovs@194.230.155.227] has joined ##hplusroadmap 18:15 -!- darsie [~kvirc@84-114-73-160.cable.dynamic.surfer.at] has quit [Ping timeout: 256 seconds] 18:28 -!- dr-orlovsky [~dr-orlovs@194.230.155.227] has quit [Quit: My MacBook has gone to sleep. ZZZzzz…] 20:31 -!- yashgaroth [~ffffffff@172.58.19.145] has quit [Quit: Leaving] 21:17 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-bkoviwysqewympxa] has quit [Quit: Connection closed for inactivity] 23:15 -!- orange_ [~textual@92.223.89.136] has joined ##hplusroadmap --- Log closed Fri Mar 13 00:00:23 2020