--- Log opened Sat Mar 18 00:00:14 2023
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06:44 < hprmbridge> lachlan> It’s a meme
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07:29 < kanzure> (like how people joke adam3us carries around his backpack because it has satoshi's keys)
07:35 < hprmbridge> CheekyMonkey> I still think Adam3us has the highest chance of being Satoshi from all the people alleged to be Satoshi
07:39 < hprmbridge> ComputingBanana.btc> How come?
07:41 < hprmbridge> ComputingBanana.btc> An Australian friend of mine has been told about BTC by Craig since 2008… (I refuse to believe it’s Craig 🤣)
08:51 < hprmbridge> msnewgooty> Huge. Figuring out the transcription factors that can enable transdifferentiation of mesenchyme to ABPC is a direct line of sight for regeneration
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11:30 < fenn> "norovirus outbreak linked to raw oysters" .. thanks oyster people, thanks a lot
11:36 < fenn> so we're allowed to criticize chinese people for eating bats and pangolins or whatever, but icky slimy sewage bathing molluscs are just fine and dandy
11:36 < fenn> raw even
11:36 < fenn> it's a disaster waiting to happen
11:39 < fenn> (ABPC = antler blastema progenitor cells)
11:42 < fenn> how many people are in the discord now?
12:12 -!- BEXCHA is now known as Mabel
13:06 < fenn> whole body fluorescent labeling (wildDISCO) mouse atlas video: https://twitter.com/erturklab/status/1627615842073452544
13:07 < hprmbridge> kanzure> yeah there has to be some advancements for whole body clearing
13:07 < fenn> i think this is the same protocol https://www.nature.com/articles/s41596-022-00788-2?WT.mc_id=TWT_NatureProtocols
13:09 < hprmbridge> kanzure> 89 on discord
13:34 < fenn> "No cutting needed, no metal needed, no clearing needed, X-ray holographic nano-tomography (XNH) can image mm size samples (mouse cortex & adult Drosophila brain etc) with sub-100-nm resolution. You just need access to a synchrotron"
13:34 < fenn> maybe some day we can get the radiation dose down to where it can be used in-vivo
13:52 < muurkha> maybe Craig Wright is pretending to be Satoshi in order to protect the real Satoshi, who either is his friend or has paid him enough money to compensate for destroying his reputation
13:54 < L29Ah> maybe satoshi is you, and you try to switch the attention to Craig Wright to avoid the satoshiness
13:54 < L29Ah> s/switch/redirect/
13:58 < muurkha> right, fo exampler
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14:09 < L29Ah> ah, i made it unclear
14:09 < L29Ah> maybe satoshi is you, and you try to redirect the attention to Craig Wright's friends or otherwise acquaitance to avoid the satoshiness
14:10 < fenn> ""Wolverine" - it gives your python scripts regenerative healing abilities!
14:10 < fenn> Run your scripts with it and when they crash, GPT-4 edits them and explains what went wrong. Even if you have many bugs it'll repeatedly rerun until everything is fixed"
14:10 < fenn> it's a really good idea and also a really bad idea
14:11 < L29Ah> fun idea
14:11 < fenn> really needs to show you the changes and ask to proceed each and every fail/retry step
14:11 < L29Ah> make sure to run in a sandbox
14:12 < fenn> "imagine running your failing tests and it fixes them by writing the implementation"
14:15 < hprmbridge> kanzure> yes the synchrotron brain imaging stuff is cool https://twitter.com/kanzure/status/1611203821887229954
14:15 < hprmbridge> kanzure> russell seems to think there's a way to do imaging of individual receptors with x-ray
14:16 < hprmbridge> kanzure> dunno if he has a way to do it without a lethal dose of x though
14:16 < fenn> gold nanoparticles right
14:16 < hprmbridge> kanzure> ya
14:16 < hprmbridge> kanzure> btw he says he can come
14:16 < L29Ah> what's up with neutrino tomography?
14:17 < L29Ah> too big angular size of the neutrino source to be useful?
14:19 < fenn> did you mean neutron tomography?
14:22 < L29Ah> no
14:24 < fenn> neutrino interactions with matter are many orders of magnitude too low to give any contrast, especially with the low detection efficiency of neutrino sensors which are gigantic tanks of clear liquid surrounded by photomultiplier tubes
14:25 < fenn> apparently the US military uses mobile neutrino detectors to sniff out nuclear warheads, so the tech is making progress
14:26 < fenn> this is something the size of a van
14:41 < kanzure> neutrino tomography is when you wait for neutrinos to pass through the earth?
14:41 < L29Ah> yes
14:53 < muurkha> L29Ah: Indeed, that would be a good strategy if I were Satoshi, which is why I was suggesting Satoshi might be employing it
14:54 < muurkha> the crucial thing here is that Craig Wright's own doings are mostly responsible for all the attention he's been receiving
14:54 < muurkha> I think mobile neutrino detectors work by irradiating things with neutron beams to provoke fission
14:58 < fenn> i don't believe that satoshi would sue bitcoin developers or cause them to be sued. it sets a bad precedent
14:59 < muurkha> as satoshi you've already had to relinquish most of the potential power you could have had to not cause bad things to happen
15:00 < muurkha> first by virtue of creating Bitcoin, then by virtue of disappearing
15:07 < muurkha> maybe "had to" is wrong.  maybe it was avoidable!  but anyway the architecture of Bitcoin and the disappearance of Satoshi each individually amounted to substantial voluntary relinquishments of power
15:08 < L29Ah> could have burned his bitcoins just as a gesture of goodwill
15:09 < L29Ah> so a lil more likely he got dragged from the internets by the problems in meatspace
15:09 < muurkha> like a fatal neurological disease
15:14 < fenn> suing developers is not the same as relinquishing power. it actively destroys the network itself, by preventing needed fixes from happening
15:14 < fenn> and discouraging improvements
15:15 < kanzure> see https://news.ycombinator.com/item?id=34685029
15:16 < muurkha> letting other people sue developers, even if you think that is bad, is an instance of relinquishing power
15:18 < fenn> but that is not what you were claiming
15:18 < kanzure> "... programmable addition via site-specific targeting elements (PASTE). The patent describes site-specific integration of a nucleic acid into a genome, using a CRISPR–Cas9 nickase fused to a reverse transcriptase (RT) and a serine integrase" https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/11572556
15:19 < kanzure> from:
15:19 < kanzure> "Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases" https://www.nature.com/articles/s41587-022-01527-4
15:19 < kanzure> pretty sure jonathan gootenberg follows me? or is it a different gootenberg? https://twitter.com/jgooten
15:20 < kanzure> "pulling apart the double helix like a wishbone"
15:21 < kanzure> https://www.genengnews.com/topics/genome-editing/paste-dont-cut-genome-editing-tool-looks-beyond-crispr-and-prime/
15:29 < muurkha> fenn: I said that maybe Craig Wright is pretending to be Satoshi in order to protect the real Satoshi, who either is his friend or has paid him enough money to compensate for destroying his reputation
15:29 < muurkha> obviously Craig Wright is not himself Satoshi
15:31 < fenn> is this really the first CRISPR-directed insertion method? it's hard to believe
15:32 < hprmbridge> kanzure> this is probably something like dCas9 fusion protein with an integrase
15:32 < hprmbridge> kanzure> that's my assumption at least.
15:32 < hprmbridge> kanzure> I could read it and check, but I won't.
15:32 < kanzure> (dcas9 = deactivated cas9)
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16:15 < hprmbridge> nmz787> Russell Durret?
16:18 < kanzure> http://russellhanson.com/
16:36 < kanzure> "Sulfonylation of RNA 2′-OH groups" https://pubs.acs.org/doi/full/10.1021/acscentsci.2c01237 
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17:26 < hprmbridge> CheekyMonkey> this youtube video (https://www.youtube.com/watch?v=XfcvX0P1b5g&t=3s) provides a lot of supporting evidence for Adam and heard from a reputable source it's not Nick Szabo (who I thought was also likely)
18:16 < hprmbridge> yashgaroth> yeah it's just prime editing but with an added integrase. So you use prime editing to insert the integrase recognition site wherever you want, and the integrase uses that site to pop the transgene in. Prime editing was already just an nCas9 (nickase mutant, not quite dCas) fused to an RT, which works well for short sequence insertion. But integrase recognition sites are short(er than a whole gene)
18:16 < hprmbridge> yashgaroth> so that insertion would be pretty efficient. The behemoth in this paper is an nCas9+RT+integrase fusion, I guess having it all together might help localize the integrase to the site
18:22 < hprmbridge> yashgaroth> doesn't seem that groundbreaking, surely someone has used prime (or HDR or w/e) to put an integrase att site into the genome and followed it up with a regular integrase insertion. The fusion part might be 'novel' but not 'non-obvious' in that case. Anyway it still leaves scars from the residual halves of the att site, which isn't ideal but it has some use cases
18:37 < kanzure> AAV capsid design firm https://www.dynotx.com/
18:46 < hprmbridge> msnewgooty> Also check out Capsidia, Voyager, 4DMT, Apetura
18:46 < hprmbridge> msnewgooty> I think I’ve missed a few
18:49 < hprmbridge> msnewgooty> Also LogicBio and Capsigen
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19:08 < hprmbridge> kanzure> do you think there is any hope for deep tissue adult gene therapy ?
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19:26 < hprmbridge> msnewgooty> Yeah. I think we’ll have muscle soon. Heart potentially
19:27 < hprmbridge> msnewgooty> For AAV, brain is a big focus
19:27 < hprmbridge> msnewgooty> I’m pretty excited about immune modulation. Get B cells to make custom biologics and knock in new payloads as needed
19:37 < hprmbridge> kanzure> @CheekyMonkey pause that video at 12m 38sec and you'll see me
20:55 < kanzure> https://scifind.io/posts/ultra-cheap-pcr-precipitation-using-peg-and-salt-0agbj0sy13cr8ua
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--- Log closed Sun Mar 19 00:00:14 2023