--- Log opened Sun May 07 00:00:54 2023
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07:34 < nsh> now uses GPT4 by default. each  .gpt without a -c resets the channel context, whereas -c continues it by appending (USER:, ASSISTANT: pairs) -s will tell current context token length (max 8k atm)
07:35 < hprmbridge> kanzure> .gpt brainstorm several ways of doing whole body cryonics with supercooled gas
07:35 < hprmbridge> kanzure> oh yeah, the bridge.
07:35  * nsh can fix so responds over bridge
07:35 < nsh> .gpt brainstorm several ways of doing whole body cryonics with supercooled gas
07:36 < nsh> generation may be quite slow compared to 3.5 turbo
07:37 < nsh> or else bugs :)
07:37 < gpt4paste> ​1. Nitrogen gas chamber: Create a specialized chamber filled with supercooled nitrogen gas, which would rapidly cool the body to cryogenic temperatures. The subject would be placed in a protective suit and then immersed in the chamber, where the temperature would be gradually lowered to minimize thermal shock.2. Helium immersion: Similar to the nitrogen gas chamber, but using - http://sprunge.us/mFcOQ8
07:38 < hprmbridge> kanzure> that's weird. I thought the gas ideas were usually about replacing the blood with gas.
07:39 < nsh> *expectation management intensifies*
09:11 < docl> .gpt -c remember, you need to cool the inside of the body, not just the outer layer. think step by step.
09:12 < gpt4paste> ​2. Helium immersion: Similar to the nitrogen gas chamber, but using supercooled helium gas instead. Helium has a higher thermal conductivity than nitrogen, which would allow for more efficient cooling of the body's interior. The subject would be placed in a protective suit and then immersed in the chamber, where the temperature would be gradually lowered to minimize thermal sho - http://sprunge.us/DvFur0
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14:44 < hprmbridge> kanzure> https://zuzalu.city/
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14:46 < alethkit> No tax advantages. Shame.
14:46 < Jay_Dugger> Hello, everyone.
14:47 < L29Ah> cryptohipster.city
14:48  * L29Ah lives 50km from zuzalu
14:48 < L29Ah> well not really city, just a pack of events at a luxurious hotel
15:13 < hprmbridge> kanzure> "Viable offspring derived from single unfertilized mammalian oocytes" https://www.pnas.org/doi/10.1073/pnas.2115248119
15:18 < muurkha> which gases have the lowest thermal conductivity?
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15:19 < L29Ah> ask google?
15:28 < alethkit> muurkha: First guess would be the noble gases
15:30 < alethkit> apparently silica aero gel
15:30 < alethkit> which is not a gas.
15:31 < alethkit> Well, figure out how to get around CFC bans?
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16:06 < kanzure> .gpt Write the protocol to activate parthenogenesis in mouse somatic cell.
16:06 < gptpaste> ​Parthenogenesis is a process of reproduction in which an egg cell develops into an embryo without fertilization by a sperm. In mice, parthenogenesis can be induced in somatic cells using specific protocols. Here is a general protocol to activate parthenogenesis in mouse somatic cells:1. Obtain somatic cells from the mouse of interest, such as skin or blood cells.2. Culture t - http://sprunge.us/NljOPU
16:19 < kanzure> okay, mitotic inhibitors, such as colcemid or nocodazole and then calcium ionophore A23187 for activation. not so bad.
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16:24 < nsh> (i rewrote the bot for economy, use .gpt4 specifically to use that model)
16:24 < nsh> (as the request warrants)
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19:57 < fenn> https://www.youtube.com/watch?v=4PUIxEWmsvI
19:57 < fenn> .t
19:57 < EmmyNoether> WORLD OF TOMORROW by DON HERTZFELDT - YouTube
21:20 < muurkha> SF6 isn't a CFC
21:20 < muurkha> .gpt4 Write the protocol to activate parthenogenesis in mouse somatic cell.
21:22 < gptpaste> ​Parthenogenesis is a form of asexual reproduction in which an egg develops into an embryo without being fertilized by sperm. While parthenogenesis occurs naturally in some species, it has not been observed in mammals like mice. However, researchers have been able to induce parthenogenesis in mouse eggs through various experimental techniques.Here is a protocol to activate par - http://sprunge.us/DnRP1n
21:38 < fenn> the gpt bot would be more economical if it were instructed to skip the preamble
21:38 < fenn> for one-turn exchanges it doesn't use very many tokens tho
21:39 < fenn> it's when you have a long back and forth that the cumulative token count goes way up
21:45 < fenn> is this protocol legit? all you have to do to start embryogenesis is stimulate calcium oscillations by dunking it in SrCl2 solution?
21:46 < muurkha> I wish I knew
21:47 < fenn> simple facts like this are often buried and inaccessible in modern science
21:47 < muurkha> it may not be that simple
21:48 < fenn> any paper you can actually get is about "advanced X" and assumes you have already read the original paper about "X" which is nowhere to be found
21:49 < muurkha> even GPT-4's answer is a bit more complex:
21:49 < muurkha>    - Transfer the oocytes (either enucleated or with somatic cell nuclei) into the activation medium containing SrCl2 and cytochalasin B.
21:49 < muurkha>    - Incubate the oocytes in the activation medium for 1-6 hours at 37°C in a humidified atmosphere with 5% CO2.
21:49 < muurkha>    - The SrCl2 induces Ca2+ oscillations, mimicking sperm entry, while cytochalasin B prevents cytokinesis and polar body extrusion.
21:49 < muurkha> but that could be bullshit
21:50 < fenn> and it's a Ca2+-free culture medium
21:50 < muurkha> right
21:50 < muurkha> I've often found "advanced X" papers to be a good source for summaries of the original paper about X
21:51 < muurkha> in the Introduction and Related Work sections
21:51 < muurkha> dissertations are even better
21:52 < fenn> https://en.wikipedia.org/wiki/Parthenogenesis#Methods
21:53 < muurkha> > Reports of human parthenogenesis have famously existed since ancient times, featuring prominently in Christianity and various other religions.
21:55 < fenn> shh that's a "miracle" not some smelly biological thing
21:56 < fenn> the obvious counterpoint is that jesus was male
21:57 < fenn> perhaps mary was also XY karotype and androgen insensitive
21:58 < muurkha> wouldn't she be infertile then?
21:59 < fenn> we are rationalizing here.
22:03 < muurkha> oh yes.
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23:25 < fenn> "Although Hwang Woo-Suk deceived the world about being the first to create artificially cloned human embryos, he contributed a major breakthrough to stem cell research by [accidentally] creating human embryos using parthenogenesis."
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--- Log closed Mon May 08 00:00:54 2023