--- Log opened Tue Sep 05 00:00:13 2023
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06:13 < kanzure> hello thenickname
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13:09 < hprmbridge> kanzure> hello @bootstrap3141
13:42 < hprmbridge> msnewgooty> @kanzure what do you know about the minicircle follistatin component of Blueprint? What's the delivery mechanism
14:01 < docl> https://minicircle.io/our-therapies/
14:02 < hprmbridge> msnewgooty> Yeah. No info on plasmid delivery, it seems
14:03 < hprmbridge> msnewgooty> Gonna assume it's either electroporation or some LNP formulation
14:06 < kanzure> msnewgooty: i'm hoping it's not minicircle
14:06 < kanzure> their claims are a little wacky
14:07 < kanzure> maybe he has a different team and a different approach
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14:20 < hprmbridge> msnewgooty> this is just mind boggling though...it is really something he could inject
14:21 < hprmbridge> msnewgooty> or do at least mRNA/LNP
14:21 < hprmbridge> msnewgooty> little hard to evaluate with incomplete information from him, though
14:25 < kanzure> there's a few issues
14:26 < kanzure> like mass manufacturing of minicircles at scale for human-relevant doses
14:26 < kanzure> as for "reversibility" is it "inducible expression" 
14:27 < hprmbridge> msnewgooty> Well the episomal dna itself does get lost over time, expression wise
14:27 < kanzure> when i talked with them they mumbled something about miRNA silencing.. yeah.
14:28 < hprmbridge> msnewgooty> It’s hard to know, honestly.
14:28 < hprmbridge> msnewgooty> Minicircles aren’t even the best technology of this class. Nanoplasmids are easier in my experience
14:35 < kanzure> yashgaroth will chime in, in a bit
14:36 < kanzure> i had him stay at the lab a bit later to catch a meeting and now he is stuck in traffic, it's my fault
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14:53 < hprmbridge> yashgaroth> based on the phrasing of bryjo's announcement, it seems like they're going with minicircle. Mac mentioned what they were using a few years ago, I think tet-off with one of those reinforcing dealies where it turns off permanently after a dose of tetracycline
14:54 < hprmbridge> yashgaroth> their numbers were unbelievable (in the bad way), like expression going up between 1 and 3 months post-injection. Even though the mechanism for that with a non-integrating plasmid would be ?????
14:56 < hprmbridge> yashgaroth> like sure minicircles are less likely to be silenced long-term, but it's not this magic bullet they claim it is. They were always very sketchy on details
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15:18 < muurkha> yashgaroth: sounds exciting
15:33 < kanzure> "Surveillance is even more important than laws." - george church (2023)
15:34 < kanzure> from https://docs.google.com/document/d/1rPvdwJbcz6mdBIniBk9Rhzqch37zbRW2ho4bWxRQ31Y/preview
15:34 < kanzure> oh, 2022
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16:28 < kanzure> from https://htgaa2022.notion.site/HTGAA-2022-d39e5560ad83483ab87d415f085b60c6
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16:54 < L29Ah> https://manifold.markets/ArunJohnson/will-300-lifespan-extension-in-roti longevity research via monopoly money prediction market
17:13 < fenn> gene editing company with TWO images of backwards DNA on the home page.. nah
17:14 < muurkha> ahaha
17:16 < fenn> (minicircle.io)
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--- Log closed Wed Sep 06 00:00:14 2023