--- Log opened Fri Sep 29 00:00:36 2023
01:26 < hprmbridge> kanzure> "Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore" https://www.nature.com/articles/s41592-023-02021-8
01:30 < hprmbridge> kanzure> "Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases" https://www.biorxiv.org/content/10.1101/2023.09.12.557440v1
01:30 < hprmbridge> nmz787> Fenn i bought and played that Cad circuit game... I'm tempted to call it career development or job related, in which case I just worked overtime playing it :/
01:30 < hprmbridge> kanzure> see https://twitter.com/JulianeWeller/status/1707410771741733353
01:31 < hprmbridge> kanzure> "Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses" https://www.science.org/doi/10.1126/sciadv.adk0171
01:31 < hprmbridge> kanzure> https://twitter.com/jgooten/status/1707496012082725326
01:32 < hprmbridge> kanzure> "Fanzor is a eukaryotic programmable RNA-guided endonuclease" https://www.nature.com/articles/s41586-023-06356-2
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04:03 < sphertext_> rate my improvised steamer lol https://imgur.com/a/9wkys8k
04:04 < sphertext_> the main challenge was to let some steam escape, because otherwise the water underneath would bubble up around the edges where the sieve made contact with the pot and then and spill over... 
04:06 < sphertext_> takes about 1h to fully cook soy beans. and it retains far more nutrients, than boiling them would. you should never boil anything (unless you can utilise the broth afterwards) 
04:07 < sphertext_> nearly half the nutrients are flushed into the broth and most ppl throw it away..
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04:46 < TMA> sphertext_: not retaining the broth is sometimes desirable, if for example you want to get rid of some water soluble compounds that you don't want to eat (like soaking beans and throwing out the soak water, because you get rid of the flatulence inducing oligosacharides)
04:48 < TMA> sphertext_: nixtamalization is another desirable "boiling" with throwing out the "broth"
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09:16 -!- Topic for #hplusroadmap: biohacking, nootropics, transhumanism, open hardware | this channel is LOGGED: http://gnusha.org/logs | https://diyhpl.us/wiki | not quite sponsored by george church | banned by the MIT media lab and the FDA
09:16 -!- Topic set by fenn [~fenn@user/fenn] [Fri Mar 25 06:29:19 2022]
09:16 -!- Irssi: #hplusroadmap: Total of 72 nicks [1 ops, 0 halfops, 0 voices, 71 normal]
09:16 -!- mode/#hplusroadmap [+v gnusha] by ChanServ
09:16 -!- Channel #hplusroadmap created Wed May 19 06:51:36 2021
09:19 -!- Irssi: Join to #hplusroadmap was synced in 139 secs
09:27 < hprmbridge> nmz787> doesn't nixtimalization include alkaline water too? Unless you're one of those crazy "alkaline blood pH" people, I'm not sure anyone wants to retain that liquid for consumption
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11:01 < sphertext_> i know what soaking beans does. i was talking about boiling them after soaking, or boiling potatoes, vegetables etc lol
11:01 < sphertext_> or even throwing away the aquafaba from canned products
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12:12 < TMA> sphertext_: steaming potatoes instead of boiling them even makes them tastier, IMHO. Sorry, 'twasn't apparent to me what's the extent of your broth-retention-exhortation
12:14 < TMA> nmz787: indeed. it involves boiling zea mays kernels in CaOH or KOH solution.  that's why I endorse not consuming the "broth"
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13:26 < hprmbridge> nmz787> Any programmatic cad folks in here that want a job with my team at Intel? Software engineer / CS heavy, with Fab hobby interest is ideal
13:26 < hprmbridge> nmz787> Being in Oregon ideal, but not required
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13:45 < hprmbridge> nmz787> https://jobs.intel.com/en/job/hillsboro/eda-tools-software-engineer/41147/54296386752
13:45 < hprmbridge> nmz787> Tell your friends
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14:37 < L29Ah> i love writing code for openscad, do i qualify?
14:37 < L29Ah> no academic credentials
14:43 < hprmbridge> nmz787> I've been mostly using gdstk
14:43 < hprmbridge> nmz787> We have a whole internal api with helper functions for more parametric stuff
14:44 < hprmbridge> nmz787> I'm first interviewer, send me resume
14:44 < hprmbridge> nmz787> Are you in US?
14:50 < L29Ah> nope
16:15 < TMA> the description reads like "very stressfull job"
16:49 < L29Ah> very corpo job
16:50 < L29Ah> nmz787: https://0x0.st/HVGL.pdf
16:50 < L29Ah> all the EDA i used yet were concerned with circuits and PCBs, never silicon
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17:14 < hprmbridge> nmz787> Tma yeah it's a corporate job, but it's the best team I've ever been on. Extremely exciting stuff IMO. Not too stressful, very accommodating and team and org is very concerned with work life balance
17:15 < hprmbridge> nmz787> URL Description is generic, I said the real job
17:15 < hprmbridge> nmz787> Get cad done, have fun and profit
17:16 < hprmbridge> nmz787> L29Ah are you in an ITAR restricted country right now? That could make things quite difficult.
17:22 < L29Ah> nmz787> nah
17:22 < L29Ah> montenegro
17:56 < kanzure> https://www.cambridgeindependent.co.uk/news/is-it-time-to-change-law-on-genome-editing-of-human-embryos-9332731/
18:03 < hprmbridge> Katylase> *singing* Crispr-cas9, bring me a gene, encoding for a specific protein, make a few snips at this coded locus, you work so well inside a Streptococcus... ^^
18:06 < hprmbridge> Katylase> Every time gene editing gets mentioned, it reminds me of this song🤭
18:13 < sphertext_> found some concrete examples of licensing/permits that are required by online vendors of bio stuff https://www.atcc.org/support/order-support/permits-and-restrictions
18:15 < sphertext_> still wondering what the requirements for obtaining a permit from e.g USDA... do you just fill in a form to explain what you want and why you need it, how you're going to use it, etc? 
18:15 < kanzure> usually one would consult a regulatory lawyer with these questions, or someone who has done it before. or you can just fill it out and see what happens yeah.
18:17 < sphertext_> well i figured there's a reasonable chance someone in here might have tried to purchase funky GMOs before :) 
18:19 < sphertext_> and related: "Bed Bugs are for professionals only. We do not sell bed bugs unless you own a trained scent detection canine or are a Pest Control Professional. No exceptions. Verification of your business is required."
18:20 < sphertext_> apparently you have to pass an exam to get the permit lol 
18:21 < sphertext_> some permits require demonstrable long term experience in the industry... hmm. this one can be tricky 
18:22 < sphertext_> there's also training courses that issue a diploma, or you can use a relevant bachelor's degree 
18:22 < sphertext_> yeah, seems pretty hard to circumvent tbh
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18:35 < L29Ah> what exactly do you need tho?
18:36 < L29Ah> pretty sure most/all diybio stuff isn't locked behind the "long term experience in the industry" thing
18:38 < L29Ah> for example https://www.the-odin.com/all-products/
18:39 < sphertext_> atm nothing specific. i just have an abstract interest in how it all works. because i do anticipate wanting restricted things again in the future
18:39 < sphertext_> wow some of that stuff is pretty cool
18:40 < superkuh> https://www.aphis.usda.gov/aphis/newsroom/stakeholder-info/sa_by_date/sa-2023/rsr-light-bio-petunia Genetically modified glowing Petunia approved for sale in the sense that they say non-food genetically engineered plants aren't regulated (by them). "These genes provide a metabolic cycle to produce light via luciferin monooxygenation. Green light (530 nm) is emitted continuously by the plants at a low level, most prominently from young flowers."
18:42  * L29Ah waits for non-food sweet-protein yeast and non-food tooth-plaque-outcompeting bacteria offers
19:41 < hprmbridge> gourneau> Cool AR avatars https://www.youtube.com/watch?v=MVYrJJNdrEg
19:50 < fenn> we need a copyleft style ATCC alternative repository of organisms
19:50 < fenn> it's too bad there is literally no way to do this, if the goal is to reproduce science experiments
20:08 < fenn> it's strange that there are so few bioluminescent plants in nature
20:08 < fenn> you'd think the moth pollinated plants would be all over that
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23:02 < sphertext_> i feel like there would be a massive demand on the black market for transgenic novelty pets 
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--- Log closed Sat Sep 30 00:00:37 2023