| --- Day changed Sat Oct 11 2008 | ||
| kanzure_ | oh god | 00:01 |
|---|---|---|
| kanzure_ | I'm going to hate myself for this | 00:01 |
| kanzure_ | but it occurs to me that branding openmanufacturing with a play on "MADE IN AMERICA" might be worth pursuing. | 00:02 |
| kanzure_ | "MADE WITH AMERICA" perhaps. | 00:03 |
| kanzure_ | not sure if this is just fear mongering by a wild man ranting, but evidently there's no vertical PCB manufacturer left in the US | 00:08 |
| kanzure_ | http://manufacturingnews.com/news/08/0930/DuBois.html | 00:08 |
| drazak | you ought o be able to use the DMD buffers for electrophorisis and PCRs right? | 01:00 |
| drazak | http://www.med.yale.edu/genetics/ward/tavi/p06.html | 01:00 |
| fenn | what's a DMD buffer? | 01:08 |
| fenn | am i reading this right? optimal dNTP concentration in the reaction = 6000 mM | 01:09 |
| fenn | 6M is like, fuming acid | 01:09 |
| fenn | fuming nucleic acid lol | 01:09 |
| fenn | i'd just use the standard buffer | 01:11 |
| drazak | the standard buffer doesn't have as good a resolution | 01:12 |
| fenn | no, that gel is just overexposed or maybe they put too much PCR product in | 01:13 |
| drazak | it looks like they were exposed the same | 01:14 |
| drazak | and, from my photography knowledge | 01:14 |
| drazak | that's not overexposure | 01:14 |
| fenn | but the standard buffer has 1.6x as much in it | 01:14 |
| fenn | anyway, _my_ gels never looked like that, and i never used a "DMD" buffer whatever that is | 01:15 |
| drazak | mhm | 01:15 |
| drazak | I might just use the standard one then | 01:15 |
| drazak | It's cheaper | 01:15 |
| drazak | and the parts are easier to find | 01:15 |
| fenn | probably because it uses 30x less dNTP's | 01:15 |
| drazak | s/parts/chemicals/ | 01:15 |
| fenn | mercaptoethanol is stinky too | 01:16 |
| drazak | aye | 01:16 |
| drazak | it's hard to find | 01:16 |
| fenn | i bet all lab chemicals are hard to find | 01:16 |
| kanzure_ | yep | 01:17 |
| kanzure_ | unless you have deep university pocket books | 01:17 |
| fenn | drazak: what are you some kind of terrorist? making drugs? | 01:17 |
| * fenn pokes drazak aggresively | 01:17 | |
| drazak | fenn: not... really | 01:17 |
| drazak | rofl | 01:17 |
| kanzure_ | fenn: you don't remember drazak? | 01:17 |
| drazak | I'm into biochem | 01:17 |
| fenn | its just the standard routine, americans cant fathom someone doing chemistry for any reason other than bombs or drugs (probably because that's all anyone makes in america any more) | 01:18 |
| drazak | nah | 01:18 |
| drazak | bombs are definitely not my style | 01:18 |
| kanzure_ | uh oh, I must not be in a good mood, I'm returning to procurement issues again. | 01:18 |
| kanzure_ | apparently Dow leechfeeds all of their chemicals from other manufacturers in a few cases | 01:19 |
| kanzure_ | their hydrocarbons come from bioreactor byproducts | 01:19 |
| fenn | leechfeeds? | 01:19 |
| kanzure_ | leeches? | 01:19 |
| kanzure_ | but not really leeching since evidently they pay for these industrial byproducts | 01:19 |
| fenn | if you can't use byproducts in other processes you have to dispose of them == $$$ | 01:19 |
| kanzure_ | I'm sure they have giant plants somewhere in europe or something | 01:19 |
| kanzure_ | I wonder if there's a mindat.org but for manufacturing and supply chains. | 01:20 |
| fenn | alibaba? | 01:20 |
| kanzure_ | mindat is for minerals, how about something for the other layers of the ecosystem | 01:20 |
| kanzure_ | nah, that's an ebay thingy | 01:20 |
| fenn | thomasnet? | 01:20 |
| kanzure_ | whatever happened to my thomasnet rip? | 01:20 |
| * kanzure_ wonders if it's still running | 01:21 | |
| fenn | i guarantee you won't find a giant flow diagram of all the materials and products in the world | 01:21 |
| fenn | many companies work very hard to keep that info secret | 01:21 |
| fenn | bleh.. resume resume | 01:22 |
| drazak | kanzure_: oh hey, you mentioned that you had some other stufd about PCR and gel electrophorisis than what's in the old zip I grabbed, I haven't grabbed from get yet | 01:23 |
| kanzure_ | drazak: Few more moments. | 01:26 |
| kanzure_ | fenn: gah, I should have remembered how much I hate patrick | 01:27 |
| kanzure_ | "Dammit man! I'm not talking about information, I'm talking about physical reality!" - patrick on openmanufacturing | 01:27 |
| kanzure_ | as if physical reality wasn't information | 01:27 |
| kanzure_ | what the fuck is wrong with that guy? | 01:27 |
| drazak | patrick who | 01:27 |
| kanzure_ | hell if I know | 01:27 |
| kanzure_ | Anderson | 01:27 |
| fenn | omg web forms, when will i learn | 01:29 |
| fenn | never write something in a web form you dont have backed up elsewhere | 01:29 |
| kanzure_ | resume? :/ | 01:29 |
| fenn | ya | 01:29 |
| kanzure_ | "I sit around all day complaining about what yer doin' wrong with the internets" | 01:29 |
| fenn | it never quite has the same ring when you write it the second time | 01:30 |
| kanzure_ | (or maybe that's me) | 01:30 |
| fenn | where's my internet bitching award? | 01:30 |
| kanzure_ | you sir do not deserve the internet bitching award | 01:30 |
| fenn | i need to put it on my resume! | 01:30 |
| kanzure_ | that's something you'd send if you were applying to replace Roland | 01:30 |
| kanzure_ | that's his name, right? xkcdguy | 01:30 |
| fenn | meep? | 01:31 |
| fenn | BOFH? | 01:31 |
| kanzure_ | ? | 01:31 |
| fenn | go and acquaint thine self with the annals of our bastard operator from hell | 01:32 |
| kanzure_ | god I want to punch patrick | 01:38 |
| kanzure_ | it's like he assumes 'designs' are just bits and bytes | 01:38 |
| kanzure_ | in reality, good designs correlate map to territory | 01:38 |
| fenn | STEP AWAY FROM THE KEYBOARD before you add a drop in the bucket of internet flamedom | 01:41 |
| fenn | he has a point though, men with guns jealously guard 99% of the resources out there | 01:42 |
| fenn | or something like that | 01:42 |
| * kanzure_ had to go down to get laundry | 01:43 | |
| fenn | i managed to regurgitate my resume, so it's all good | 01:43 |
| bkero | fenn: 'eh? | 01:43 |
| kanzure_ | so, the thing I don't like about patrick's approach is that he's a secret front for futiity | 01:43 |
| fenn | i really hate this stuff though, it feels like doing taxes | 01:43 |
| kanzure_ | "well, since we can't get anything, guess we just have to do this socially" | 01:43 |
| bkero | xkcd guy is randal munroe | 01:44 |
| fenn | "socially" != socialism | 01:44 |
| kanzure_ | or, rather, I should say that he's imposing his bullshit about money and economics on to something that doesn't need it | 01:44 |
| drazak | xkcd guy is awesome | 01:44 |
| kanzure_ | fenn: I don't care if it != socialism | 01:44 |
| drazak | :D | 01:44 |
| fenn | i'm just sick of ranting about communism on that list | 01:44 |
| fenn | and ranting about libertarianism on sifter, while i'm bitching | 01:44 |
| kanzure_ | I wasn't aware of rants about communism | 01:44 |
| kanzure_ | you're on sifter? | 01:44 |
| fenn | i lurk | 01:44 |
| kanzure_ | did I point you to sifter? | 01:45 |
| fenn | ya | 01:45 |
| kanzure_ | gee, I'm good | 01:45 |
| fenn | heh i had name-drop you to get in :| | 01:45 |
| kanzure_ | funny. | 01:45 |
| kanzure_ | I would be wary of anywhere that doesn't let me in unless I had to mention my name | 01:46 |
| fenn | yeah and i mentioned that | 01:46 |
| fenn | half-seriously accusing them of being a secret cabal of masonists or whatever | 01:46 |
| fenn | masonites? haha | 01:47 |
| kanzure_ | anyway, what I was going to reply to patrick with (until kmail crashed (nick of time?)), | 01:47 |
| kanzure_ | was that a good 'design' would reach all the way from me to the materials and back again | 01:47 |
| kanzure_ | that's a good design - he suggests that I say "we just eat CAD files" | 01:47 |
| kanzure_ | but he's missing the point of technology. | 01:47 |
| fenn | of course you design for what you have | 01:48 |
| fenn | his point is that we dont really have anything | 01:48 |
| kanzure_ | if he wants to install Capitalism 2.0 or his Socialism 2.0 (I don't know what he's pushing, but whatever it is, it's bullshit) | 01:48 |
| kanzure_ | then let him install that. | 01:48 |
| kanzure_ | well, of course we don't have anything, it's because people like him keep complaining about Those Damn Capitalists. | 01:48 |
| kanzure_ | classic Somebody Else's Problem Field. | 01:48 |
| kanzure_ | ok. maybe not so much of a classic example. | 01:49 |
| drazak | :P | 01:49 |
| kanzure_ | Alright. I'm calming down. Don't care that much about these guys anyway .. they are not architects. | 01:50 |
| drazak | not to be greedy or anything, but does that mean you can grab me those links now? | 01:51 |
| * kanzure_ glances at opera and sees BOFH open. | 01:51 | |
| kanzure_ | Yes. | 01:51 |
| kanzure | http://www.separationsnow.com/coi/cda/home.cda?chId=0 | 01:52 |
| kanzure | http://www.marinebiotech.org/gelelectro.html | 01:52 |
| kanzure | http://www.scq.ubc.ca/the-macgyver-project-genomic-dna-extraction-and-gel-electrophoresis-experiments-using-everyday-materials/ | 01:52 |
| kanzure | 01:52 | |
| kanzure | http://www-saps.plantsci.cam.ac.uk/worksheets/scotland/dna.htm | 01:52 |
| kanzure | http://www.scienceteacherprogram.org/biology/NLee05.html | 01:52 |
| fenn | "it's not illegal to look for job applicants with high IQ, but if you actually ask an applicant their IQ, that fact can be used against you in a discrimination suit." is this for real? | 03:26 |
| kanzure_ | people ask iq? | 03:26 |
| kanzure_ | This is like a flashback to California. They were .. obsessed with their IQs. Trying to prove that they weren't doomed because of their IQ variables. | 03:27 |
| gene | percent? | 04:26 |
| gene | you there? | 04:26 |
| gene | or whatever your nick is now? | 04:26 |
| gene | you're right about HF | 04:28 |
| bkero | Selling laptop | 05:11 |
| gene | is opera more secure than firefox? | 05:12 |
| bkero | Heh heh | 05:13 |
| gene | I think my parents are hacking me | 05:14 |
| bkero | lol | 05:15 |
| bkero | hax'd | 05:15 |
| gene | yeah | 05:16 |
| -!- nsh- is now known as nsh | 08:43 | |
| ybit | bkero: you're selling your new dell laptop so soon? | 12:46 |
| ybit | "[05:11] <bkero> Selling laptop" | 12:46 |
| bkero | ybit: No, selling macbook | 15:31 |
| ybit | ah | 16:35 |
| * ybit wants it | 16:35 | |
| * ybit wants it | 16:35 | |
| ybit | or just an msi wind | 16:36 |
| ybit | or asus eeepc | 16:36 |
| ybit | weirdest thing, almost every house in the neighborhood is havng a yardsale, so it's like a flea-market outside | 16:37 |
| ybit | speaking of, i now have an extra keyboard and 2 mice | 16:39 |
| ybit | for free of course, i like to bargan *evil smile* | 16:40 |
| ybit | damn the "i" on this keyboard | 16:40 |
| fenn | damn your i's! | 16:42 |
| kanzure_ | woah | 19:45 |
| kanzure_ | Sudden outbreak of awesome by Jason on the diybio mailing list. | 19:45 |
| ybit | [ kanzure_ ] are you referring to "Bio Bench project wiki request for comments and additions" | 19:48 |
| ybit | there were a wealth of links presented | 19:48 |
| kanzure_ | yes | 19:52 |
| kanzure_ | for the record | 19:54 |
| kanzure_ | http://www.genocad.org/ which seems to be what we were thinking of | 19:55 |
| kanzure_ | http://pobol.org/ for RDF export of biobricks repository | 19:55 |
| kanzure_ | (with Google Group link and svn link) | 19:55 |
| kanzure_ | http://bricklet.org/ | 19:55 |
| kanzure_ | wonder if they have SBML compatability. | 19:56 |
| kanzure_ | Hey, I have an idea. | 20:22 |
| ybit | pobol and bricklet are old, but i hadn't seen genocad and http://openwetware.org/wiki/Computational_Tools | 20:22 |
| kanzure_ | Abandoned mines. | 20:23 |
| kanzure_ | Abandoned mines are ridiculously safe when you don't use humans. | 20:23 |
| kanzure_ | Plus they serve as proof-of-concept. | 20:23 |
| kanzure_ | bkero: Was your tank try when you added your biofuel? | 20:28 |
| kanzure_ | *dry | 20:28 |
| kanzure_ | http://minerals.usgs.gov/minerals/pubs/mapdata/ | 20:33 |
| kanzure_ | It would be interesting to use biofuels to power a robotic army for automated abandoned mine mining tasks .. use the remaining left over materials for cargo-container delivery. | 20:40 |
| kanzure_ | (delivery to open source projects, no less) | 20:40 |
| kanzure_ | http://tin.er.usgs.gov/mrds/mrds.zip 30 MB of possibly interesting information. I have a few more minutes on the download until I get to peak. | 20:42 |
| kanzure_ | Yay I'm actually motivated to work on the bioreactor. | 20:44 |
| willPow3r | kanzure_, what kind of bioreactor? | 20:48 |
| willPow3r | photobioreactor? | 20:49 |
| kanzure_ | 10,000 gallon photobioreactor + an unknown lipid extraction methodology as of yet. | 20:49 |
| kanzure_ | Multi growth scheduling. | 20:49 |
| willPow3r | neato | 20:50 |
| willPow3r | unk lipid extraction method? what are you looking at? | 20:50 |
| * kanzure_ looks for his list. | 20:50 | |
| kanzure_ | Acid/solvent extraction, sonification, high pressure lysing, electromechanical pressure, supercritical method w/ flogas, microwave, enzymatic lyses, liquid nitrogen. | 20:52 |
| willPow3r | those tend to be rather limited in their efficacy | 20:52 |
| kanzure_ | I haven't ran the numbers yet. | 20:52 |
| kanzure_ | Anything I should add to the list? | 20:52 |
| willPow3r | i went to a lecture here @ UCSD on the subject of growing algae (biomass) in a photobioreactor for conversion to biofuel | 20:54 |
| willPow3r | and the topic of lipid extraction was talked about | 20:55 |
| kanzure_ | http://heybryan.org/mediawiki/index.php/Bioreactors | 20:55 |
| willPow3r | trying to find the link | 20:55 |
| kanzure_ | Really lipid extraction is the big issue. | 20:55 |
| kanzure_ | photobioreactors, meh; algae *will* grow | 20:55 |
| kanzure_ | (yes, though, getting awesome growth is always, well, awesome) | 20:55 |
| willPow3r | right | 20:55 |
| kanzure_ | But these things are built for growth. ;-) | 20:55 |
| willPow3r | Brian Gregory Mitchell | 20:56 |
| kanzure_ | "numerical modeling of yield optimization" yep | 20:58 |
| kanzure_ | Sounds like my kind of guy. | 20:58 |
| kanzure_ | Scripps Photobiology Group | 20:58 |
| willPow3r | you should ask him out on a date | 20:59 |
| willPow3r | get a couple drinks in him | 20:59 |
| kanzure_ | http://spg.ucsd.edu/People/Greg/BGM_Publications.htm | 20:59 |
| kanzure_ | "absorption cross section" <-- Probably falls off with the square of distance. | 20:59 |
| willPow3r | yea, i was just looking @ that | 20:59 |
| kanzure_ | That's an interesting way to model it. But. Lipid extraction modeling is more important at this point. | 21:00 |
| kanzure_ | Part of the problem is that the algae we have been cultivating have this nasty hard membrane. | 21:00 |
| willPow3r | and breaking down that membrane requires lots of energy | 21:01 |
| willPow3r | so its similar to the hydrogen fuel cell problem | 21:01 |
| kanzure_ | Gah, it's been a while since I've remembered what the hydrogen fuel cell problem is. I seem to recall it was something about efficient storage. | 21:02 |
| willPow3r | what percentage of fuel extraction is possible using current top manufacturing techniques? | 21:13 |
| kanzure_ | Refinery efficiencies? | 21:15 |
| kanzure_ | Usually you don't care much about that since your energy input is zilch .. it's all stored petrochemicals from a bajillion years ago. | 21:16 |
| kanzure_ | Although I'm sure they do care anyway. | 21:16 |
| fenn | thermal depolymerization | 21:21 |
| ybit | i like this article: http://en.wikiversity.org/wiki/Wikiversity:Free_education_and_free_school%3F | 21:28 |
| ybit | http://en.wikiversity.org/wiki/Category:Wikiversity as well | 21:29 |
| ybit | wrong channel | 21:31 |
| kanzure_ | yay I'm so happy | 21:31 |
| ybit | what happened? | 21:32 |
| kanzure_ | http://fedoraforum.org/forum/archive/index.php/t-185682.html algOS - oil from algae, open source | 21:32 |
| ybit | ooooh | 21:32 |
| kanzure_ | Narsi Santhanam narsi@esource.in | 21:33 |
| kanzure_ | fuck | 21:33 |
| willPow3r | i've seen this before | 21:33 |
| kanzure_ | They have nothing | 21:33 |
| kanzure_ | Screw this shit. I could write a simulator faster than it'd take Narsi to reply .. | 21:34 |
| kanzure_ | .. or the microcontroller code for operating a reactor. | 21:34 |
| ybit | "Since they are not part of the human food chain at present, ..." oi, i eat spirulina | 21:35 |
| kanzure_ | So I'm still not finding much in the way of studies of lipid extraction techniques from algae. | 21:35 |
| kanzure_ | One source is suggesting electromagnetic extraction. | 21:35 |
| kanzure_ | which I guess is my 'microwave' that I listed above | 21:36 |
| kanzure_ | (electromagnetic extraction got me curious because of the link on the page to algOS) | 21:36 |
| willPow3r | http://www.ucsd.tv/search-details.asp?showID=14378 | 22:05 |
| kanzure_ | Dj Polarny - Sylwek Dp Megamix 2004 isn't terrible. | 22:12 |
| kanzure_ | Hrm. Narsi is an interesting fellow. | 22:18 |
| kanzure_ | http://esource.in/ | 22:18 |
| willPow3r | i hate ice cream trucks. | 22:26 |
| drazak | kanzure_: those links were mostly all just gelling, not really PCR | 22:30 |
| kanzure_ | One moment. | 22:33 |
| kanzure_ | New batch of links. | 22:34 |
| kanzure_ | http://heybryan.org/~bbishop/docs/pcr.txt | 22:35 |
| kanzure_ | The sciam link would be a good start. | 22:35 |
| drazak | right, I have that printed out | 22:35 |
| kanzure_ | dkjflajkd;fjkasdf | 22:35 |
| drazak | the sciam link | 22:36 |
| drazak | that is | 22:36 |
| kanzure_ | I don't know if it ha s content, but the how-2-diy link claims to have videos | 22:36 |
| kanzure_ | You should be able to buy a cheap thermocycler if you really need it, something using the Pierett process; the reagents are an unsolved mystery as far as I know. Are you needing links to commercial suppliers? | 22:37 |
| kanzure_ | They usually come in 'kits'. | 22:38 |
| kanzure_ | (I know this because labmates fight over the kits all the time. :-)) | 22:38 |
| drazak | lol | 22:39 |
| drazak | well | 22:39 |
| drazak | I'm going to make my own buffer | 22:39 |
| drazak | but yeah, some links to commercial suppliers would be nice | 22:39 |
| drazak | there's a link to making a thermoscycler, which I had planned to do to start | 22:39 |
| drazak | :D | 22:39 |
| kanzure_ | I'll ask my bio buddies for their favorite kits. | 22:40 |
| kanzure_ | This would be a good question to pose to the diybio.org mailing list though. "Hi, give me teh kits." | 22:40 |
| drazak | lol | 22:41 |
| kanzure_ | willPow3r, fenn - for lipid extraction, what about killing the algae via having 'dead tanks'? Remove all nutrients, remove all light sources, let it sit for some amount of time. Once it's all dead biomass, apply low-scale extraction process - sonification, acid-solvent extraction, then typical transesterification techniques to get to the goods. | 22:44 |
| kanzure_ | Hard to select for algae that dies quickly, though. | 22:44 |
| willPow3r | the problem is breaking down cellulose at a high rate | 22:48 |
| willPow3r | dead algae doesn't make that process any easier | 22:48 |
| willPow3r | not only that, some algae actually excrete the desired products | 22:51 |
| kanzure_ | Not this stuff. But names? | 22:51 |
| kanzure_ | Huh. Google search very lucrative. | 22:53 |
| willPow3r | http://en.wikipedia.org/wiki/Botryococcus_braunii | 22:54 |
| kanzure_ | Largest algae collection in the world right here on campus, and with full access. | 22:55 |
| kanzure_ | wtf, I started pidgin 30 minutes ago | 22:57 |
| drazak | I think I have 83 tabs in firefox | 22:57 |
| kanzure_ | Firefox will crash soon :) | 22:58 |
| kanzure_ | bkero was doing 180 | 22:58 |
| kanzure_ | I keep trying to tell him that opera does 300 no sweat. | 22:58 |
| drazak | nah | 22:58 |
| drazak | my laptop has 2 gig of memory | 22:58 |
| willPow3r | i've lost too much information by having that many tabs | 22:58 |
| kanzure_ | I wish that mattered. | 22:58 |
| kanzure_ | willPow3r: crashes? | 22:58 |
| willPow3r | yep | 22:58 |
| kanzure_ | yay session restore hacked into autosave | 22:58 |
| kanzure_ | (you don't want it to restore, just to autosave) | 22:59 |
| willPow3r | like when my gf watches the hills | 22:59 |
| kanzure_ | ((why repeat what fucked you up?) | 22:59 |
| kanzure_ | ) | 22:59 |
| willPow3r | and inevitably does not reopen last session | 22:59 |
| willPow3r | craig venter is amazingly boring | 23:01 |
| kanzure_ | news? | 23:01 |
| * kanzure_ has a venter number of 2. in terms of faces. | 23:01 | |
| willPow3r | watching a lecture he gave on genomic advances | 23:01 |
| kanzure_ | ah | 23:02 |
| kanzure_ | date? | 23:02 |
| willPow3r | 5/7/08 | 23:02 |
| kanzure_ | And it's boring? | 23:02 |
| willPow3r | i ran out of adhd meds | 23:02 |
| willPow3r | so i'm relying on no-doz alone | 23:02 |
| willPow3r | glue coas | 23:06 |
| willPow3r | i guess hes not that boring. | 23:09 |
| kanzure_ | Botryococcus doesn't grow all that quickly. I'm looking around to see if its hydrocarbon secretion complexes have been sequenced. | 23:10 |
| willPow3r | thats what she said | 23:11 |
| kanzure_ | I can't imagine hydrocarbon secretion to be efficient for rapidly replicating critters. | 23:15 |
| kanzure_ | I can't imagine hydrocarbon secretion to be efficient for rapidly replicating critters. | 23:16 |
| willPow3r | then alter genetic sequence for thin cell wall | 23:17 |
| kanzure_ | Ideally the amount of genetic modification should be minimized. | 23:18 |
| kanzure | " Thiruvananthapuram (IANS): Weather permitting, Chandrayaan 1, India's first mission to the moon, will lift off from the Satish Dhawan Space Centre at Sriharikota in Andhra Pradesh Oct 22, Indian Space Research Organisation (ISRO)" | 23:18 |
| fenn | pffffff BUY A THERMOCYCLER??!!!! | 23:21 |
| kanzure | Such magic! the heat, but then the cold! | 23:22 |
| willPow3r | sounds like something billy mays could handle | 23:22 |
| kanzure | I very much want to go to Odessa, Florida so that I can go into _his_ home and yell at Billy Mays to buy my products. | 23:23 |
| fenn | who is this person that breaks into your house to harass you? | 23:23 |
| willPow3r | that would be one of the most amazing acts every | 23:24 |
| kanzure | fenn: television. it's another realm. ignore. | 23:24 |
| fenn | oh i havent seen one of those in years | 23:24 |
| willPow3r | s/y/ / | 23:25 |
| fenn | do they still make them? | 23:25 |
| kanzure | billy mays? nah | 23:25 |
| kanzure | discontinued. | 23:25 |
| fenn | i think it would be neat to have a tag cloud of statistically improbable phrases being automatically generated from an irc chat | 23:27 |
| kanzure_ | It would be neat in general to have the backend machinery for Amazon's "statistically improbable phrases" in general .. | 23:28 |
| fenn | then it could scroll by on a big LED sign | 23:28 |
| kanzure_ | ? | 23:28 |
| kanzure_ | such as? | 23:28 |
| willPow3r | spam email subjects represent a similar phenomenon | 23:28 |
| fenn | the red dot matrix character array | 23:28 |
| kanzure_ | fenn: no, what sort of crap are you expecting to see scroll by ? | 23:28 |
| fenn | the phrases | 23:29 |
| kanzure_ | to be honest, irc has a lot of statistic improbabilities | 23:29 |
| kanzure_ | like any of the guys getting women. | 23:29 |
| fenn | snap | 23:29 |
| willPow3r | not in the field of metabolic engineering | 23:29 |
| kanzure_ | Hrm? | 23:29 |
| ybit | http://www.hubmed.org/ | 23:29 |
| kanzure_ | http://pubget.org/ | 23:30 |
| kanzure_ | hrm | 23:30 |
| fenn | http://nubged.org/ | 23:30 |
| kanzure_ | http://pubget.com/ and http://pubget.org/mobile | 23:30 |
| ybit | ooh | 23:30 |
| * ybit likey | 23:30 | |
| kanzure_ | You're welcome. | 23:30 |
| ybit | hai, arigatou | 23:31 |
| kanzure_ | que? | 23:31 |
| ybit | si, gracias | 23:31 |
| kanzure_ | robot? | 23:31 |
| willPow3r | no worky | 23:31 |
| kanzure_ | willPow3r: try the .com | 23:31 |
| willPow3r | beautiful. | 23:31 |
| kanzure_ | http://pubmedcentral.gov/ is beautiful in general. | 23:32 |
| drazak | kanzure_: did you ever say the links to some commercial suppliers? | 23:32 |
| kanzure_ | http://heybryan.org/projects/autoscholar/ has had a link to pubget for a few months now, ybit | 23:32 |
| kanzure_ | drazak: Friends have not replied yet. | 23:32 |
| drazak | what about for general stuff? | 23:32 |
| kanzure | invitrogen, carolina biologic supply, IDT, | 23:33 |
| kanzure | oh wait, I think there's a list in the git repo | 23:34 |
| kanzure | http://heybryan.org/gitweb.cgi?p=biotech.git;a=tree | 23:34 |
| kanzure | 'Good list of vendors.html" | 23:34 |
| kanzure | http://heybryan.org/gitweb.cgi?p=biotech.git;a=blob;f=Good%20list%20of%20vendors.html;h=52558e4de48302b9e13f70491c9aaabaaad1320a;hb=HEAD | 23:34 |
| kanzure | http://heybryan.org/gitweb.cgi?p=biotech.git;a=blob_plain;f=Good%20list%20of%20vendors.html;hb=HEAD | 23:35 |
| kanzure | Blah, nevermind. | 23:35 |
| kanzure | Oh wait. Yep. Vendors. | 23:35 |
| kanzure | BioSupplyNet - 2700 biotech companies, Ambion, Amersham Pharmacia Biotech, Beckman Coulter, Beckman Coulter Liquid Handlers (Biomek/Multimek), Bio-Rad, Brinkman Instruments, Corning Labware & Equipment, Epicentre, Fisher, Gelman, Glen Research, ICN, IDT (Integrated DNA Technologies), Invitrogen, Kimble Kontes, Life Technologies (GIBCO BRL), MidWest, MJ Research, Molecular Dynamics, Nalge Nunc, NEB, Operon, Perkin Elmer, PerSeptive Biosystems, Pierce, | 23:37 |
| kanzure | Operon, Promega, Qiagen, Shimadzu, Sigma Aldrich, Stratagene, VWR | 23:37 |
| kanzure_ | I remember we were using Ambion kits. | 23:37 |
| drazak | cool | 23:39 |
| fenn | between sigma aldrich and invitrogen you can get just about everything | 23:39 |
| fenn | perkin elmer has equipment but you cant afford it | 23:39 |
| kanzure | funny, that's what sigma aldrich's asking price is | 23:39 |
| kanzure | (everything) | 23:39 |
| fenn | i figured they were just all unreasonably expensive | 23:40 |
| kanzure | actually, I take that back, I'm glad sigma aldrich at least posts their prices on their site | 23:41 |
| drazak | kanzure_: where can I get primers and such? | 23:42 |
| kanzure | You can order them commercially, or you could order a sequence to be synthesized by our friends at http://e-oligos.com/ for $0.35/bp | 23:43 |
| drazak | bp or kp? | 23:44 |
| kanzure | http://www.primerfox.com/ <-- tool for primer generation | 23:44 |
| kanzure | basepair. | 23:44 |
| kanzure | yeah .. | 23:44 |
| drazak | because I saw a place doing it for like 45c/kbp | 23:44 |
| kanzure | djkfal;kfjklda;jfal | 23:44 |
| kanzure | linkage? | 23:44 |
| drazak | hold on | 23:44 |
| kanzure | I've heard of Korean labs doing it dirt cheap | 23:44 |
| drazak | http://www.mrgene.com | 23:45 |
| kanzure | Awesome. Thanks. | 23:47 |
| drazak | for up to 3kbp | 23:47 |
| kanzure_ | http://mrgene.com/synbio looks like they're at the hong kong conference | 23:50 |
| fenn | it just says 49 cents up to 3kb | 23:51 |
| kanzure_ | http://genscript.com/ does $0.55/bp .. | 23:51 |
| kanzure_ | up to 50kbp. yay. | 23:52 |
| fenn | what's the error rate? | 23:52 |
| kanzure_ | they guarantee 100% match... hrm. | 23:53 |
| fenn | how the heck do they make a 50kbp sequence? | 23:53 |
| kanzure_ | hrm | 23:53 |
| fenn | For non-complex gene sequences up to 3 kb, we offer discount rates of as low as $0.55/bp (EUR0.40/bp). | 23:54 |
| kanzure_ | ah, only $0.55/bp for up to 3 kbp | 23:54 |
| kanzure_ | heh | 23:54 |
| fenn | is there a wikipedia page with this info? | 23:55 |
| kanzure | http://www.biocompare.com/matrix/124/Gene-Synthesis.html | 23:55 |
| fenn | GET QUOTE GET QUOTE GET QUOTE GET QUOTE | 23:56 |
| fenn | someone please fire the person who came up with that | 23:57 |
| willPow3r | i thought you just came up w/ it | 23:57 |
| kanzure_ | willPow3r: check the link .. | 23:57 |
| kanzure_ | or don't | 23:57 |
| kanzure_ | it's useless. | 23:57 |
| willPow3r | indeed it is | 23:58 |
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