| --- Day changed Sun Mar 01 2009 | ||
| xp_prg | no, I have a refrigerator with food in it | 00:00 |
|---|---|---|
| xp_prg | I just put the stuff in there | 00:00 |
| kanzure | fenn: dpkg was yakking about a dependency to libvtk5-qt4 specifically. | 00:00 |
| kanzure | xp_prg: I would strongly discourage mixing biological material storage with food storage. | 00:01 |
| Gene_H | you need about 3 peltier coolers | 00:01 |
| fenn | wah | 00:01 |
| Gene_H | stack them on top of each other | 00:01 |
| Gene_H | power up | 00:01 |
| Gene_H | put in fridge | 00:01 |
| kanzure | don't put in fridge | 00:01 |
| kanzure | blargh. | 00:01 |
| * kanzure worries. | 00:01 | |
| Gene_H | gets down to very cold | 00:01 |
| xp_prg | dude I just did | 00:01 |
| Gene_H | -60 freezer temp | 00:01 |
| xp_prg | so like what temp does it need to be at? | 00:02 |
| fenn | put ice in a bucket | 00:02 |
| Gene_H | Kanzure don't worry about it, I've done stuff like that and nothing bad ever happened to me | 00:02 |
| fenn | or styrofoam box if you're fancy | 00:02 |
| kanzure | xp_prg: pop quiz. is there anything wrong with storing your gel runs in a refridgerator? | 00:02 |
| xp_prg | don't know, but I am just storing the raw contents that came in the box | 00:02 |
| Gene_H | xp_prg description of what you are doing? | 00:02 |
| kanzure | specifically a refrigerator that you use for food | 00:02 |
| * kanzure reads the elmergui manual | 00:03 | |
| kanzure | http://www.nic.funet.fi/index/elmer/doc/ElmerGUIManual.pdf | 00:03 |
| katsmeow-afk | just an fyi you can discard, but if you do this on a larger scale, some dept stores now sell compact fridges for under $100 | 00:03 |
| xp_prg | Gene_H I ordered this: | 00:04 |
| xp_prg | Items | 00:04 |
| xp_prg | Item# ItemDescription QtyShipped | 00:04 |
| xp_prg | 211081 TRANS, GREEN GENE 4 STN KIT 1 | 00:04 |
| xp_prg | 211081A TRANS, GREEN GENE 4 STN KIT 1 | 00:04 |
| xp_prg | 211082C TRANS, GREEN GENE4&8STN REFILL 1 | 00:04 |
| Gene_H | heh | 00:04 |
| Gene_H | LABEL IT AS NOT FOOD | 00:04 |
| kanzure | Gene_H: you're an idiot | 00:05 |
| kanzure | that's not the point | 00:05 |
| xp_prg | so like do you think the cells died and stuff? | 00:05 |
| kanzure | fenn: wash your hands of this diybio shit while you still can | 00:05 |
| Gene_H | well how'd they ship it? | 00:05 |
| xp_prg | in a box | 00:05 |
| Gene_H | with? | 00:05 |
| Gene_H | dry ice? | 00:05 |
| xp_prg | no | 00:05 |
| fenn | kanzure: there are some smart people, they'll figure out how to make it work | 00:05 |
| Gene_H | well you may not need to | 00:05 |
| kanzure | ever wonder why biology labs mount fucking turrets next to food refrigerators and shoot anyone with anything remotely looking like biological materials? | 00:05 |
| katsmeow-afk | yes | 00:06 |
| Gene_H | kanzure did you hear the story about the people who developed artificial urine? | 00:06 |
| kanzure | Gene_H: artificial urea? | 00:06 |
| xp_prg | hello is my stuff messed up or not? | 00:06 |
| fenn | xp_prg: go fuck yourself | 00:06 |
| Gene_H | yeah for calibrating urine testing machines | 00:06 |
| kanzure | yay fenn. | 00:06 |
| xp_prg | hahhaha | 00:06 |
| xp_prg | how come? | 00:07 |
| kanzure | xp_prg: I don't think you understand us | 00:07 |
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| kanzure | did I or did I not mention any health hazards in the last few minutes | 00:07 |
| fenn | you're such an ignorant demanding twit | 00:07 |
| xp_prg | dude is my shit fucked up or not? | 00:07 |
| Gene_H | xp_prg, you idiot, you have to use staphlococcus | 00:07 |
| kanzure | another pop quiz: is "your shit being fucked up" the biggest of your worries? | 00:07 |
| xp_prg | dude I just paid 80 bux for this thing | 00:07 |
| kanzure | what does that have to do with what I just asked you | 00:07 |
| xp_prg | its in containers and stuff I am sure it is safe while in the containers right? | 00:08 |
| xp_prg | I didn't open the little containers or anything | 00:08 |
| Gene_H | so what are you going to do next? | 00:08 |
| kanzure | Gene_H: please stop for the moment | 00:08 |
| kanzure | this is kind of serious. | 00:08 |
| xp_prg | well I am going to take it sf next weekend | 00:08 |
| Gene_H | ok, it seems you have no idea as to what you are doing | 00:10 |
| Gene_H | so the best way to preserve biological samples is to add a cryoprotectant | 00:12 |
| xp_prg | ok do you think my cells died and stuff though? | 00:13 |
| Gene_H | a cryoprotectant allows biological samples to be stored at higher temperatures | 00:13 |
| Gene_H | common household bleach is a great cryoprotectant | 00:13 |
| Gene_H | if used in small quantities | 00:13 |
| fenn | huh. dpkg -i foo aborted and then apt-get -f install will get all the required packages for foo | 00:13 |
| kanzure | fenn: yes, that happened to me too | 00:14 |
| kanzure | I found it strange that it knew to do that | 00:14 |
| Gene_H | if you use it in high quantities your cells die | 00:14 |
| Gene_H | dilute it to about 20% | 00:14 |
| kanzure | Gene_H: quantities? volumes maybe? | 00:14 |
| Gene_H | yes volumes | 00:14 |
| Gene_H | less than a drop should do | 00:16 |
| Gene_H | remember, IT HAS TO BE LESS THAN A DROP or it doesn't work | 00:21 |
| kanzure | http://web.mit.edu/elmer_v5.3/tutorials_files/ hm. Maybe I'll use some of these. | 00:22 |
| Gene_H | firefox crashed again | 00:23 |
| kanzure | too many tabs. | 00:23 |
| fenn | it seems elmerf90 wants to compile a file every time? | 00:23 |
| kanzure | every time what? | 00:23 |
| fenn | for each simulation | 00:23 |
| xp_prg | you guys are so hard to get help from | 00:23 |
| fenn | we are not DIYbio tech support | 00:24 |
| fenn | try #diybio (gasp) | 00:24 |
| fenn | or better yet, read a book | 00:24 |
| Gene_H | xp_prg, you have to add a cryoprotectant for you to store it in your refrigerator | 00:25 |
| fenn | kanzure: the elmerfem-doc package has some nice pdf's | 00:26 |
| xp_prg | Gene_H does it need to be refrigerated or not? | 00:26 |
| xp_prg | it says store in a dry cool place | 00:26 |
| kanzure | fenn: where are they put? /usr/share/blah/ ? | 00:26 |
| fenn | /usr/share/doc/elmerfem-doc/ | 00:26 |
| fenn | btw dpkg -S is your minion | 00:27 |
| kanzure | aha. | 00:27 |
| fenn | i should mention apt-file while i'm at it | 00:27 |
| Gene_H | IT NEEDS TO BE REFRIGERATED, BUT YOU HAVE TO ADD A CRYOPROTECTANT | 00:28 |
| fenn | oh FFS the reason i was having a hard time is it's ElmerGUI not elmergui | 00:28 |
| Gene_H | if you don't add a cryoprotectant it just dies | 00:28 |
| xp_prg | dude I don't hae a cryoprotectant shit | 00:29 |
| fenn | cells will survive at room temp but they won't stay competent | 00:30 |
| * xp_prg kills himself | 00:30 | |
| xp_prg | well I just put it in the fridge I hope that helps | 00:30 |
| kanzure | that's what we've been getting at. | 00:30 |
| fenn | aw man it's one of those apps that segfaults if you look at it wrong | 00:32 |
| kanzure | yeah :( | 00:32 |
| kanzure | but apparently there are some tools under the hood that should be used anyway | 00:32 |
| kanzure | if I can figure out how. looking at those PDFs now. | 00:32 |
| kanzure | or first the examples. | 00:32 |
| Gene_H | WHAT'S THIS! NOW ADOBE ACROBAT IS CRASHING ON ME! | 00:35 |
| kanzure | ooh, ElmerTutorials.pdf *is* nice. | 00:36 |
| Gene_H | and I can't read it, because ADOBE is broken | 00:37 |
| kanzure | you don't even have dpkg | 00:37 |
| fenn | it seems like it runs but i dont get any colors in the result (just all blue) | 00:53 |
| kanzure | that's farther than I've gotten | 00:53 |
| kanzure | how'd you manage to get there? | 00:53 |
| kanzure | neat, there's a t-cross microfluidic tutorial | 00:56 |
| fenn | copy /usr/share/elmerfem-doc/tutorials_files to your ~ | 00:56 |
| katsmeow-afk | wierd, i just had to kill adobe too, it was hung but using 97%cpu forever | 00:56 |
| fenn | then open TemperatureAngleGUI/angle.grd | 00:56 |
| fenn | then run-> run solver; then start postprocessor and postprocess and open case.ep | 00:56 |
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| fenn | i dunno how to run the non-GUI tutorials | 00:58 |
| kanzure | hrm. | 00:58 |
| kanzure | okay, I see the all-blues. | 00:58 |
| fenn | i guess i should follow the tutorial pdf | 00:58 |
| kanzure | fenn: the TemperatureAngleGUI demo seems to only be showing the first timestep | 00:59 |
| kanzure | erm, nevermind | 00:59 |
| kanzure | the output file is data3d.ep according to one of the model config menus. | 01:00 |
| kanzure | HeatSolve: Result Norm : 7.78240309139357489E-002 | 01:02 |
| kanzure | HeatSolve: Relative Change : 2.34494291199996476E-011 | 01:02 |
| kanzure | E-011, geeze. | 01:02 |
| xp_prg | what you guys doing? | 01:03 |
| fenn | hm crashes every time i click on 'edit solver settings' in the equations window | 01:03 |
| kanzure | same here. | 01:03 |
| fenn | ok i'll just not do that | 01:04 |
| fenn | where do i set density and heat conductivity? | 01:04 |
| kanzure | model -> material | 01:04 |
| kanzure | I never see anything like heat* under model->equation even though some screenshots show that there should be something | 01:07 |
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| kanzure | I think the "edit solver settings" crash bug is probably critical to all of this, fenn. | 01:14 |
| kanzure | http://elmerfem.wiki.sourceforge.net/Getting+started+with+ElmerGUI might give a hint as to what "Edit Solver Settings" is for. | 01:14 |
| fenn | they should just provide a .sif file | 01:15 |
| kanzure | some of the sif files seem to have weird paths in ! lines at the top | 01:17 |
| kanzure | which might be why the non-GUI tutorials do not work | 01:17 |
| kanzure | erm, except for not having the file you actually need | 01:18 |
| kanzure | (grd) | 01:18 |
| kanzure | ah neat, RayleighBernardGUI now runs | 01:18 |
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| Gene_H | ok kanzure, I' | 01:26 |
| Gene_H | accidently a whole adobe acrobat | 01:27 |
| fenn | ok well this has ceased being fun | 01:33 |
| fenn | i thought from the pretty screenshots that it would .. work | 01:34 |
| kanzure | seems to be working for me (it's at time=400) | 01:34 |
| kanzure | (in the "convergence history" window) | 01:34 |
| fenn | that just means it's not a steady state | 01:35 |
| kanzure | eh. | 01:35 |
| fenn | i dont get any results because i cant define the initial conditions because the GUI is fubared and all the example files are fubared | 01:35 |
| kanzure | hm, I have 140 threads from [Elmerdiscussion] | 01:36 |
| fenn | if you're serious about trying to get it to work i think you will have to compile from source | 01:38 |
| kanzure | I wonder if there is a source package in that repo. | 01:39 |
| Gene_H | that's it, screenshots | 01:41 |
| Gene_H | I don't have to fix adobe, I can just take scrceenshots of what I'm reading | 01:41 |
| fenn | you can just take a picture of the screen with a video camera and upload it to youtube | 01:42 |
| Gene_H | that would reduce the quality | 01:42 |
| fenn | make sure to slowly pan across the text and add dramatic music | 01:42 |
| Gene_H | you know that's the way they used to make movies of video games | 01:42 |
| Gene_H | adding music would circumvent copyright | 01:43 |
| fenn | is that so | 01:43 |
| Gene_H | you know the only way to make a video of gameboy games is to use a video camera? | 01:44 |
| Gene_H | sad isn't it? | 01:44 |
| kanzure | http://heybryan.org/shots/2009-03-01_elmer.png | 01:45 |
| kanzure | bwahahah. | 01:45 |
| kanzure | Gene_H: not true. | 01:45 |
| Gene_H | I mean gameboy DS | 01:46 |
| Gene_H | yeah I guess so | 01:46 |
| kanzure | uh oh, why can't I access my server | 01:48 |
| kanzure | why can't I access anything? | 01:48 |
| kanzure | but anyway, fenn, it works. | 01:49 |
| Gene_H | because your server has just become self-aware | 01:49 |
| Gene_H | I can't access it too | 01:50 |
| Gene_H | odd, adobe crashes after a preset time period | 01:51 |
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| Gene_H | almost like it's been rigged to | 01:53 |
| Gene_H | oh pretty | 01:54 |
| Gene_H | screen shot is awesome | 01:54 |
| Gene_H | youtube? | 01:55 |
| kanzure | http://www.physicsandmusic.com/contact.php aha, I have successfully googlestalked the nano song people. | 01:57 |
| Gene_H | waht does googlestalk mean? | 01:58 |
| Gene_H | physics and music eh? | 01:58 |
| Gene_H | you know I so want to build a fluidic guitar amp | 01:59 |
| Gene_H | damn machine shop | 02:00 |
| kanzure | http://www.urbandictionary.com/define.php?term=googlestalk | 02:01 |
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| fenn | you could do a fluidic voice amp | 02:08 |
| fenn | like a kazoo but with MORE POWER | 02:09 |
| Gene_H | and more quality than a kazoo | 02:10 |
| Gene_H | kazoo's just resonator | 02:10 |
| Gene_H | perhaps it could be used as a clock in a fluidic computer | 02:11 |
| fenn | as an oscillator, but you'd want a square wave for the clock | 02:11 |
| fenn | kanzure: how long did that simulation take to run? | 02:13 |
| kanzure | 5 or 10 minutes | 02:13 |
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| fenn | did you do anything in model->setup? | 02:15 |
| kanzure | no | 02:17 |
| Gene_H | huh I guess so | 02:20 |
| kanzure | I can't seem to figure out how to change the number of generated resulting states, or whatever | 02:22 |
| kanzure | in the sif file, there's timestep-sizes=1, timestep-intervals=blah, output intervals=blah, max iterations=blah, max outputlevel = blah, | 02:23 |
| kanzure | and none of these can be knobbed into producing the apparently 4 different samples that VTK is displaying for some reason. | 02:23 |
| kanzure | this is unnecessarily complicated. | 02:23 |
| Gene_H | dang it | 02:27 |
| Gene_H | I want to read those papers but I can't | 02:27 |
| fenn | what papers? | 02:27 |
| Gene_H | the one's kanzure sent me | 02:27 |
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| fenn | huh i guess it does work after all: http://fennetic.net/pub/irc/elmergui.png | 03:28 |
| fenn | when you click on surface you have to pick a value from the menu instead of Null | 03:29 |
| fenn | still can't figure out streamlines and isosurfaces | 03:30 |
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| Gene_H | what's and isosurface? | 03:51 |
| fenn | a surface of equal magnitude within a volume | 04:01 |
| fenn | it's quite probably it doesnt work because i'm only doing 2d example files | 04:02 |
| fenn | http://fennetic.net/cadwiki/index.php?isoline | 04:09 |
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| fenn | ah finally, bash completion, how I have longed for thee | 05:38 |
| fenn | ugh, too bad it's an ugly hack | 05:43 |
| fenn | i thought it might use getopt but all the values are just hardcoded in /etc/bash_completion | 05:44 |
| bkero | Hypothesis: Lua is a pain in the ass. Experiment: http://pastebin.osuosl.org/24371 Conclusion: Yep, Lua is a pain in the ass. | 06:29 |
| UtopiahGHML | http://trac.openturns.org/ Open source initiative to Treat Uncertainties, Risks.N Statistics in a structured industrial approach | 06:37 |
| fenn | could you explain what that means? | 06:51 |
| fenn | or at least why you think we'd be interested | 06:52 |
| fenn | i understand what uncertainty is and most of the distributions | 06:53 |
| UtopiahGHML | resistance of material, thus can be used in simulations to see if a designed object will hold | 06:54 |
| UtopiahGHML | thus useful for modelling | 06:54 |
| UtopiahGHML | didn't try it myself so i wont be able to say more | 06:55 |
| fenn | honestly i think it's more work than it's worth | 06:55 |
| fenn | if something is close enough to failure for the shape of the uncertainty to matter, you should probably revise it anyway | 06:56 |
| fenn | maybe siai would be interested for their end-of-the-world scenarios | 06:57 |
| UtopiahGHML | well they use it in large projects (mil. nasa. etc) so I guess passed a certain treshold of investment it's a worthy filter | 06:57 |
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| fenn | featherless chicken http://www.nextnature.net/?p=668 | 09:52 |
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| fenn | katsmeow-afk: you know a bit about google right? why did they break searching for exact phrase even in quotes with + sign in front etc | 10:59 |
| fenn | now they entirely discard punctuation no matter what | 11:00 |
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| * nsh is frown | 11:21 | |
| nsh | they even try and correct +"singleterm" queries now | 11:21 |
| nsh | fuck them in the ear already | 11:21 |
| kanzure | programmers everywhere would jump on to any new search engine that truly allowed searching for special characters | 11:42 |
| kanzure | just saying. | 11:42 |
| kanzure | is my server up? down? | 11:43 |
| kanzure | hm. out of swap space I think. | 11:44 |
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| fenn | http://fab.cba.mit.edu/content/projects/mik/notes "machine invention kit" | 12:23 |
| fenn | i think they've been monitoring my brainwaves sometimes | 12:24 |
| fenn | "goo extrusion" | 12:24 |
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| kanzure | " | 13:25 |
| kanzure | insane machining > 100k RPM | 13:25 |
| kanzure | " | 13:25 |
| kanzure | fenn: did you get any where with elmer? | 13:25 |
| fenn | yes but just the *GUI demos | 13:28 |
| fenn | the crappy example files turned me off | 13:28 |
| fenn | i have more important code to be messing with right now | 13:29 |
| fenn | http://fennetic.net/pub/irc/elmergui.png | 13:29 |
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| fenn | hi jookos2 are you associated with elmerfem? | 13:29 |
| fenn | oh that's just nsh | 13:30 |
| kanzure | well at least you got isosurfaces working. | 13:33 |
| kanzure | or whatever is producing the rainbow coloration | 13:33 |
| kanzure | maybe I'll try recompiling elmerfem. | 13:33 |
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| kanzure | http://en.wikipedia.org/wiki/Hyperthymesia another way of describing the classic 'R' case from Russia (or whatever) of the synesthesia case with amazing memory | 13:41 |
| kanzure | oh, 'S' case - Shereshevskii | 13:42 |
| kanzure | http://mail.mems-exchange.org/mailman/listinfo/mems-talk | 13:49 |
| kanzure | oh shit. I've been missing out. | 13:49 |
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| fenn | omg a mailing list you aren't subscribed to yet! | 13:53 |
| kanzure | found it via searching for "how to make glass hydrophobic" | 13:56 |
| kanzure | (besides piranha solutions) | 13:56 |
| kanzure | PMMA acrylic glass is hydrophobic apparently | 13:56 |
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| fenn | i'd say spray with scotch-gard waterproofing, but you probably want something that will stick to hydrocarbon resins | 13:58 |
| fenn | not water-repellent per-se | 13:58 |
| kanzure | I want something that will still let sharpie be written all over it. | 14:00 |
| kanzure | http://heybryan.org/books/Manufacturing/mems-talk_archive.zip | 14:01 |
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| kanzure | http://www.reefcorner.com/SpecimenSheets/bubble_algae.htm what the hell is this? | 14:31 |
| kanzure | Valonia macroalgae | 14:31 |
| kanzure | http://en.wikipedia.org/wiki/Valonia | 14:32 |
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| kanzure | http://images.google.com/images?client=opera&rls=en&q=valonia%20bubble%20algae&sourceid=opera&oe=utf-8&um=1&ie=UTF-8&sa=N&hl=en&tab=wi | 14:33 |
| kanzure | http://www.aqualog.de/news/web75/75-11-13e.pdf | 14:34 |
| kanzure | birkholtz 1986 - "killer bubbles" - a "single pod would release innumerable algal spores" | 14:35 |
| kanzure | http://www2.hawaii.edu/~delbeek/delb5.html | 14:36 |
| kanzure | ever seen any more. Birkholz, J. (1986): Üble Parasiten, die grünen Killerkugeln. Da | 14:37 |
| kanzure | A more disturbing aspect of Pseudo- | 14:37 |
| kanzure | Birkholz, J. (1986): Üble Parasiten, die grünen Killerkugeln. Das. Aquarium 20(1),31-32 | 14:38 |
| * fenn gives kanzure some percussion maintenance | 14:38 | |
| kanzure | hm? | 14:39 |
| kanzure | that tickles. stop it. | 14:39 |
| kanzure | odd how this paper was only cited three times, all by "aqualog.de" - http://scholar.google.com/scholar?num=100&hl=en&lr=&cites=12933297891618496679 | 14:40 |
| kanzure | Valonia macrophysa - smaller clustered elongated bubbles | 14:41 |
| kanzure | Boergesenia - clustered, though less dense in numbers and elongated bubble shapes | 14:41 |
| kanzure | gah | 14:41 |
| kanzure | http://www.fishlore.com/bubblealgae.htm types: valonia macrophysa, boergesenia, ventricaria ventricosa, dictosphaeria ocellata, dictosphaeria cavernosa, | 14:42 |
| kanzure | "The voltage-dependent step of the chloride transporter of Valonia utricularis encounters a Nernst-Planck and not an Eyring type of potential energy barrier" | 14:55 |
| kanzure | wtf, voltage-clamp studies with giant algae :D | 14:56 |
| kanzure | oh, for sea water organisms I suppose that makes sense | 14:57 |
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| kanzure | hm. there's a whitesides paper where he shows a microfluidic device with screws acting as valves (manually controlled by humans) | 17:00 |
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| kanzure | why has nobody made a mf device with a polypropylene or polystyrene coupler/interconnection/"micro-to-macro" straw? | 17:31 |
| kanzure | (and that's where gene would get to use his superglue, I guess. he really wants to use superglue.) | 17:31 |
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| kanzure | Microbeads display of proteins using emulsion PCR and cell-free protein synthesis | 18:27 |
| kanzure | say, this looks familiar.. | 18:27 |
| kanzure | http://heybryan.org/mediawiki/index.php/Polymerase#Microbeads_display_of_proteins_using_emulsion_PCR_and_cell-free_protein_synthesis | 18:27 |
| kanzure | (just added) | 18:27 |
| kanzure | it didn't help that w/o meant "water/oil", not "without". | 18:29 |
| kanzure | http://heybryan.org/books/Biology/Microbeads%20display%20of%20proteins%20using%20emulsion%20PCR%20and%20cell-free%20protein%20synthesis.pdf | 18:30 |
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| kanzure | http://heybryan.org/books/papers/microfluidics/synthesis/ | 19:11 |
| kanzure | huh, I wonder if what I was observing yesterday with the fluid-filled cavity microchannel thingy, with the curvature of the water due to the four parallel lines, was actually a display of a cone of the water, i.e. the edges were all constrained, and water surface tension allowed there to be the formation of a cone. | 19:39 |
| kanzure | freezing the contraption would show if that's the case methinks | 19:39 |
| kanzure | (then just take off one of the sides and look at the ice's structure) | 19:39 |
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| kanzure | Hi any08937099. | 19:59 |
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| kanzure | Can anyone get me the name of the contractor that did the pentagon fix-ups after 2001-09-11 ? | 20:29 |
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| wrldpc | prolly halliburton >:\ | 20:53 |
| kanzure | http://tech.slashdot.org/comments.pl?sid=1144961&cid=27030617 | 21:03 |
| kanzure | RTKL | 21:06 |
| kanzure | ftp://ftp.rtkl.com/ | 21:06 |
| kanzure | aww. | 21:06 |
| kanzure | http://roobishop.weebly.com/home.html <-- aww. | 21:14 |
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| Gene | so what do you want with Hal kanzure? | 21:40 |
| kanzure | does DNA sequencing by mass make sense? | 21:52 |
| kanzure | hal? | 21:53 |
| Gene | my dad used to work for Hal | 22:11 |
| Gene | you know they have these trucks | 22:11 |
| Gene | for moving mud for oil drilling operations around | 22:12 |
| Gene | they have a band of grey on the top, so they don't have to clean them as often | 22:13 |
| Gene | Oil pipelines also run at about 17,000 psi | 22:14 |
| katsmeow1 | <blink> | 22:15 |
| kanzure | Gene: where does your dad work now? | 22:21 |
| kanzure | http://heybryan.org/mediawiki/index.php/DNA_sequencer#Microfluidic_DNA_sequencing | 22:54 |
| kanzure | (just jotting down some notes) | 22:54 |
| kanzure | 135.13 g/mol, 126.11 g/mol, 151.13 g/mol, 111.1 g/mol (A, T, G, C). Hrm. So a mass-based sequencing approach would need to be able to discriminate at the scale of 10^-23 grams. That's not much. | 23:01 |
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| kanzure | fenn: I just fwd'd you a guy who wants to pay me to make him a CNC table. | 23:31 |
| kanzure | he emailed me, I called him back as soon as I got it, we chatted, he has a small factory in San Antonio, has some cash to spend on this | 23:31 |
| kanzure | he already has the table, just needs the electronics installed or something | 23:33 |
| kanzure | also, BioMors suggested a needle to poke a hole in a plastic slide for the mf-devices. I was thinking of just jamming a straw between two slides. | 23:35 |
| Gene | whoa | 23:36 |
| Gene | we're gonna make a cnc machine | 23:36 |
| kanzure | Gene: what about the mechmate? | 23:36 |
| Gene | I thought it was in no condition to work? | 23:37 |
| Gene | what's an mf-device? | 23:37 |
| kanzure | microfluidic | 23:37 |
| kanzure | because microfluidic is way too much of a buzzword | 23:37 |
| Gene | ok, so on the sharpie mfs I just found a problem | 23:38 |
| kanzure | yeah? | 23:38 |
| Gene | how do you pump stuff throught it | 23:38 |
| kanzure | did you read the paper? | 23:38 |
| Gene | ok send it to me | 23:38 |
| Gene | never mind dont | 23:38 |
| Gene | I got it | 23:38 |
| kanzure | http://heybryan.org/books/papers/microfluidics/Performing%20chemical%20reactions%20in%20virtual%20capillary%20of%20surface%20tension-confined%20microfluidic%20devices%20-%20sharpies%20-%20nail%20polish%20-%20glass%20surfaces%20-%20hydrophobicity.pdf | 23:38 |
| kanzure | basically they made one slide very hydrophobic by soaking it in piranha | 23:39 |
| kanzure | now, if you wanted to pump it, there's a few different ways that I can imagine that happening | 23:39 |
| kanzure | (1) seal the slides together (but make sure there's still a spacer inside) | 23:39 |
| kanzure | (2) poke a hole with a needle, attach a straw, blow your lungs out | 23:39 |
| kanzure | (3) possibly use electrical conduction. | 23:39 |
| kanzure | anyway, trying it with a hydrophobic slide is a must. I just didn't do it because I didn't realize how simple a concoction piranha actually is | 23:40 |
| kanzure | it's like hydrogen peroxide plus hydrogen sulfate | 23:40 |
| kanzure | erm, it's H2SO4, not hydrogen sulfate. Sorry. | 23:40 |
| kanzure | (in a 3 to 1 ratio (3 H2SO4 to 1 parts hydrogen peroxide) | 23:41 |
| kanzure | ) | 23:41 |
| kanzure | Principles of microfluidic actuation by modulation of surface stresses http://heybryan.org/books/papers/microfluidics/Principles%20of%20microfluidic%20actuation%20by%20modulation%20of%20surface%20stresses.pdf | 23:42 |
| kanzure | Microchannels Constructed on Rough Hydrophobic Surfaces http://heybryan.org/books/papers/microfluidics/Microchannels%20Constructed%20on%20Rough%20Hydrophobic%20Surfaces.pdf | 23:42 |
| kanzure | Microfluidic manipulation via Marangoni forces http://heybryan.org/books/papers/microfluidics/Microfluidic%20manipulation%20via%20Marangoni%20forces.pdf | 23:43 |
| kanzure | An optical toolbox for total control of droplet microfluidics with lasers http://heybryan.org/books/papers/microfluidics/An%20optical%20toolbox%20for%20total%20control%20of%20droplet%20microfluidics%20with%20lasers.pdf | 23:43 |
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| kanzure | there was also one paper where they heat one side and thus create a temperature gradient for thermophoresis. | 23:44 |
| kanzure | but that seems like it would just fall into equilibrium | 23:45 |
| kanzure | and then stuff is stuck at the middle :) which is bad :) | 23:45 |
| kanzure | Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce http://heybryan.org/books/Biology/Functional%20expression%20of%20the%20taste-modifying%20protein,%20miraculin,%20in%20transgenic%20lettuce.pdf | 23:55 |
| kanzure | haha | 23:55 |
| Gene | miraculin is awesome | 23:56 |
| Gene | I've experienced it's effects | 23:57 |
| Gene | quite interesting | 23:57 |
| kanzure | am I going to find miraculin on erowid or something? | 23:57 |
| Gene | hey you want to try some? | 23:58 |
| Gene | I've got some | 23:58 |
| Gene | In handy tablet form | 23:59 |
| Gene | from thinkgeek | 23:59 |
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