2009-03-08.log

--- Day changed Sun Mar 08 2009
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geneheh I got some spam from them01:31
geneIRL spam01:31
genemail from them a while back01:31
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kanzure"Hydrophilic/hydrophobic patterned surfaces as templates for DNA arrays"01:45
genemuahahahahahahahahh01:53
genehttp://s88.photobucket.com/albums/k200/elbuenohombre89/?action=view&current=GendoIkari.jpg01:55
genethat's pretty much how I am after hearing that01:55
genewait a minute, the psp has a 480 x 272 screen01:57
genethat can't be right?01:57
kanzurehttp://ukpmc.ac.uk/articlerender.cgi?artid=1545998 01:58
kanzureRecursive construction of perfect DNA molecules from imperfect oligonucleotides01:58
genehmmmm..01:59
genedna repair01:59
genedinosaur dna is damaged01:59
genedinosaurs might be profitable...01:59
kanzureI'm trying to find a paper that gives an analysis of the sources of error and error rates in conventional oligo synthesis techniques.03:00
genedamn I need to send you that stuff03:00
kanzurehttp://en.wikipedia.org/wiki/Oligonucleotide_synthesis "s the number of errors increases with the length of the oligonucleotide sequence that is being synthesized. "03:00
kanzure[citation needed]03:00
kanzureseriously03:00
genedude why can't we bring back dinosaurs with DNA error correction?03:04
geneI belive I found a solution to head mounted display mounting03:12
genewould you like to know?03:13
geneyou put acouple of titanium mounting brackets into your skull03:14
genehttp://interactive.usc.edu/members/mark/archives/wide5.jpg03:15
geneyikes, we might not want to use a LCD screen03:16
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genehttp://api.ning.com/files/KLxUkA8D81DH2fz4hfju4aa*QmoBff1yaOFTJuEuj9AWjhwozdtCjoip5sSQ6k-k1tWeN9P85HmIHRVbD2KvaR6B9vPuPDWU/cyberpunk_hacker_by_mercikos.jpg03:18
genemight have to go all out in that case03:19
genehttp://www.jewellersequipment.co.uk/eyeglasses/LHMG.JPG03:25
geneA SOLUTION HAS BEEN FOUND03:25
kanzurehttp://scholar.google.com/scholar?as_q=&num=100&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=Khorana&as_publication=&as_ylo=1960&as_yhi=1970&as_allsubj=all&hl=en&lr=03:27
genekanzure, magnifier glasses03:34
geneare the solution03:34
genewe just put LCD's on them03:35
geneLCDs with cameras for AR03:35
genesteve mann gets 640X48003:40
geneDLP is starting to tempt me03:42
gene1400x105003:43
geneis dlp03:43
genewait a second03:43
genethat's it?03:43
genehmmm...03:43
geneI think I just realized something03:43
kanzureSyringe method for stepwise chemical synthesis of oligonucleotides http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=320704&blobtype=pdf03:47
kanzureSynthesis of a gene for human growth hormone and its expression in Escherichia coli 03:47
genewith a syringe?03:48
genehow long does that take?03:48
kanzureif you use zinc, 30 minutes per nucleotide03:51
kanzurebut if you don't, it usually takes much less apparently03:51
geneHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHHAHAHAHAHAHAHAHHAHAHAA03:51
geneno03:51
kanzuregene: become a monk! synthesize your own genome by syringe!03:52
kanzure"oh fuck, I made an error"03:52
geneat 30 minutes per nucleotide it would take OVER 9000 hrs to synthesize the ebola genome03:53
geneno joke03:53
geneor 375 dayw03:53
genedays03:53
genefew03:53
geneterrorists won't be able to do that03:53
genethey have to be 4 levels above batshit insane03:55
geneand have a lot of time03:57
kanzuresyringe method takes 13 min per nucleotide. haha.04:02
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kanzureCoriolis force?09:35
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fennfor your algae filter can't you use something like this with a really fine mesh? http://images.google.com/imgres?imgurl=http://www.home-food-processing.com/ProductImages/32164.jpg&imgrefurl=http://www.home-food-processing.com/browseproducts/-32164-Grape-Spiral-for-Food-Strainer.html&usg=__J2qQqd0XJISZEnRDixJWRE4OYMs=&h=300&w=300&sz=25&hl=en&start=14&tbnid=Iv6wrnzVaAWHgM:&tbnh=116&tbnw=116&prev=/images%3Fq%3Dberry%2Bscreen%26hl%3Den%2610:37
fennduhh sorry about the linkspam10:37
fennhttp://www.enasco.com/product/C16122N10:38
fennhttp://www.home-food-processing.com/browseproducts/-32164-Grape-Spiral-for-Food-Strainer.html10:38
fennas the algae builds up on the screen it gets scraped off and pumped out10:39
kanzureI was thinking of blockin the sensor that signals whether or not the lis is closed on my ps2 ancd then drawing a circular patter as it spins so that I can get a clean microchannel totest with.10:40
kanzure*blocking10:41
kanzure*lid10:41
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kanzure*pattern11:23
kanzure*to test11:23
katsmeow-afklo12:04
katsmeow-afkl12:04
kanzureCell lysis on a microfluidic CD (compact disc) http://heybryan.org/books/papers/microfluidics/Cell%20lysis%20on%20a%20microfluidic%20CD%20(compact%20disc).pdf12:45
kanzurehuh13:22
kanzureso apparently elmer and salomé aren't in debian because of those licensing issues of opencascade13:22
kanzureanyway, opencascade 6.3.0 has been accepted into the repos apparently.13:23
kanzure"                      In contemporary phosphoramidite oligonucleotide synthesis coupling13:27
kanzurefailure rates should be 0.5% or less per coupling event.13:27
kanzure"13:27
kanzure"                                                              the nature of coupling13:27
kanzurefailures can be found in Temsamani et al. (1995) and Hecker and Rill (1998).13:27
kanzure"13:27
kanzureHecker KH, and RL Rill 1998 Error analysis of chemically synthesized polynucleotides.13:30
kanzureBioTechniques 24: 256-260.13:30
kanzure"Deletions are most likely to result from incomplete capping or de-tritylation."13:33
kanzurehm, it might be due to the reversability of the detritylation step13:40
fennelmer uses opencascade?13:41
kanzureorigin of impurities in oligonucleotides: http://heybryan.org/books/Biology/Origin%20of%20impurities%20in%20oligonucleotides.pdf see page 2913:49
kanzurewith references :)13:49
kanzurehttp://heybryan.org/mediawiki/index.php/DNA_synthesizer#Origin_of_oligonucleotide_impurities_and_errors13:52
kanzureadded the references.13:57
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kanzuregrated array with sharpie microfluidics: scotch tape dispenser has little spikes in a row. works nicely at scraping off parallel lines. My dispenser here has 17.15:32
kanzurecan tape remove nail polish? because it can remove layers of sharpie..15:40
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fenni think heekscad might actually be useful already: http://fenn.freeshell.org/motor_mount.png18:31
transilluminatornice.18:38
transilluminatoralso, growing algae in large volumes of water is stupid18:41
transilluminatorhttp://heybryan.org/bioreactor/flat/18:41
fennindeed18:42
fenni recall reading about a tube of heat-sealed plastic that was stretched out on a roof and had circulating algae fed through it18:42
fennit was about 6 inches thick and 50 feet long18:42
transilluminatormost papers focus on photobioreactor issues like "getting light to the algae"18:44
transilluminatorwhich isn't really the real issue in these things. yes, it's important, but I feel that's easy18:44
transilluminatorharvesting is more of a big deal, since the smaller buggers aren't going to just clump together naturally in some cases18:44
fennseems like some sort of spray or waterfall would be beneficial18:44
transilluminatorand chemicals for that are sort of expensive18:44
transilluminatorhow so?18:44
fennfor aeration and temperature reduction18:45
transilluminatoranyway- those papers on flat beds seem useful. just grow it in a few cm of water, but a large surface area18:45
* fenn notes that none of those papers have photos of real installations18:45
fennoh wait, there's one18:46
transilluminatorone mentioned an installation that was tested for 12 days in Japan18:46
fennlooks pretty bad tho18:46
fennvertical flat plate = stupid18:47
transilluminatoryes, I don't know what they are thinking there18:47
transilluminatorthey must be pumping it back up to the top18:47
fenni wish these guys spent more time making working systems and less time with fancy graphs and equations18:48
transilluminatorI demoed gnuplot for two biologists the other day and they went totally ballistic and all happy-feely about how awesome 'splot' was.18:48
fennhuh?18:49
fenncan you even rotate it in 3d?18:49
transilluminatoryes18:49
fenndoes it output SVG? :)18:50
transilluminatorit outputs dxf, does that count?18:51
fennapparently it does18:51
fennset terminal svg18:51
fennset output "$file.si.svg"18:51
fennreplot18:51
transilluminatorhm, I did "set term" and looked at the list and didn't see svg there, although the interwebs tell me it does18:51
fennhave to press return to see the full list18:53
fennit works!18:56
fennthat certainly isn't x^2 though18:56
transilluminatorset xrange [0:1]18:57
fennit doesnt want to write the file until i exit19:00
transilluminatorthere's another way to write files.19:00
transilluminatorhow is it that you are only now just discovering gnuplot?19:01
fenni've used it before, i just forget all the syntax every time19:01
transilluminatorwell, no, not the syntax, I can't penalize you for that19:01
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gene_kanzure find me a tiny 640x480 display19:57
kanzureisn't this what Google is for?19:58
gene_http://www.emagin.com/products/OLEDMD/OLED_microdisplays.php19:58
gene_yeah19:58
gene_are you busy?19:58
gene_dang, dmd displays are about to get cheap19:59
gene_so here's the deal, you find a display with that rez that is cheap, and find a way to hook it up to say, my laptop, I make you a case for it20:00
kanzurea case?20:01
gene_http://www.tradeeasy.com/supplier/3690/products/p999348/usmart.html20:01
gene_ugh that's a bit big20:01
gene_yeah20:01
gene_IE the part that holds it on your head20:01
gene_and optics20:02
katsmeowMCM was selling 5 and 7 inch led color screen for cheap a while back, set in unside for an ntsc feed, had speakers too, ran on 12vdc20:02
gene_I have a cheap about 5 inch LCD screen20:02
gene_it's heavy and would cause head strain20:02
gene_used on underwater robot control system20:03
* katsmeow nods20:03
gene_http://www.tradeeasy.com/supplier/144391/products/p939908/keychain-digital-photo-frame.html20:03
gene_240X16020:03
gene_compromise20:03
kanzureI suggest finding something on ebay from a well-supported, but broken cell phone20:04
gene_good plan20:04
kanzureI wonder if people put up cell phones on ebay once their minutes are used up20:05
kanzurejust because they don't know any better.20:05
gene_that 240x160 looks tempting20:05
gene_my phone has 176*x22020:06
gene_slightly more pixels20:06
gene_but wider screen20:06
gene_optics20:07
gene_oh shit optics20:07
gene_http://www.engadget.com/2008/10/23/emagins-sxga-oled-xl-microdisplay-perfect-for-night-vision-gog/20:07
katsmeowsorry Hal, i cannot do that20:07
gene_that weird shapes gonna be hard to make20:07
kanzuregene_: how about you put this on a wiki page20:08
kanzurehttp://heybryan.org/mediawiki/index.php/Wearables20:08
gene_sure20:08
kanzuresince we've already talked about this before in here, and all of those notes have to be retrieved anyway20:08
kanzureso might as well put it in one place20:08
gene_v20:12
gene_http://www.branders.com/s/personalized-clock-frame-digital-photo-73337.html20:12
gene_12 for about $40?20:12
gene_ugh20:12
gene_http://www.promopeddler.com/03-64/square-portable-mini-digital-photo-keychain-holds-qqp191768.htm20:13
gene_damn that minimum order20:13
gene_http://www.farfromboring.com/Promotional-Product/Picture-Frames/Digital-Picture-Frames/DIGITAL-PHOTO-CLOCK-FRAME-MPD-9800-108186.html20:14
gene_request a sample ;)20:14
fenngene_: what's this about CNC eh?20:14
fennthe openmoko freerunner has a tiny 640x480 screen20:15
gene_how much?20:15
gene_is it FREE!20:15
gene_how wide is the screen?20:16
kanzurehttp://pipes.yahoo.com/pipes/pipe.info?_id=9c440036b120679b4498fcaa67200718 Google Scholar RSS/email copy20:17
kanzurehttp://www.dapper.net/dapp-howto-use.php?dappName=PapersGoogleScholar20:17
kanzurehttp://www.dapper.net/dapp-howto-use.php?dappName=GoogleScholarResultsExtraction20:17
fenngene_: like 3 inches20:17
kanzurehttp://pipes.yahoo.com/pipes/pipe.info?_id=9c440036b120679b4498fcaa6720071820:17
gene_that's HUGE!20:17
kanzurehttp://davide.eynard.it/?p=35 "I wrote this little perl script which requires two parameters: the name of the author and the paper title. It then queries Scholar and returns the number of citations for that paper. Quick and easy!"20:17
fenn65mm20:18
gene_yeah that's quite bulky20:19
fenn43mm x 58mm display aarea20:19
fennwhat did you expect?20:19
gene_http://www.holoeyesystems.com/lcos_microdisplay_specifications.html20:19
gene_expensive yet awesome20:20
* kanzure is trying to figure out if there are any 'google scholar' mashups for trends analysis etc.20:20
* fenn is trying to figure out if gene is a retard20:20
fenngene_: did you get a quote?20:21
kanzurehttp://userscripts.org/scripts/show/836  "saved searches" for google scholar20:21
gene_no20:22
gene_I should though20:22
kanzure http://userscripts.org/scripts/show/12692 "I'm addicted to 'starring' items in google reader, so I created a script that adds this option to scholar. just search the site and click on the star if a result interests you. Hit 'Starred' next to the search button to see all starred results."20:22
kanzurewhat's the point of that?20:22
kanzureuseful: http://userscripts.org/scripts/show/41663 ScienceDirect popup-blocker Opens PDFs in the same window, not a pop-up20:24
gene_you know I wonder if you can stack those tiny digital picture frames together and offset them just a bit to get a higher res display20:30
fennemagin = $1500 for two displays20:30
gene_or vibrate them like they do with dmds20:31
fenndmd's dont vibrate do they?20:32
fennnot the pixel array20:32
gene_some do20:33
fennnot the pixel grid i mean20:33
fennthe laser galvo's yes20:33
gene_yes the whole pixel array back and forth20:33
kanzurewhat the fuck20:33
kanzurehttp://en.wikipedia.org/wiki/Google_Scholar20:33
kanzureAutoScholar, an automated interface to Google Scholar for grabbing papers written in perl.20:33
kanzurehttp://heybryan.org/projects/autoscholar/20:33
kanzurehuh20:33
gene_double the amount of pixels20:33
gene_you really have to see it though to understand it20:33
fennsee what?20:34
kanzurehttp://www.scitopics.com/20:34
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gene_http://cgi.ebay.com/SONY-ELECTRONIC-VIEW-FINDER-DXF-601-LENS-CAMERA-PRO_W0QQitemZ350175018595QQcmdZViewItemQQptZLH_DefaultDomain_0?hash=item350175018595&_trksid=p3286.c0.m14&_trkparms=72%3A1205|66%3A2|65%3A12|39%3A2|240%3A131820:54
gene_electronic viewfinder20:54
kanzurehey, smari's getting slashdotted: http://slashdot.org/firehose.pl?op=view&id=367618921:11
kanzurehttp://smari.yaxic.org/blag/2009/03/06/microsoft-skull-fucks-icelands-economy-contracts-syphilis/21:11
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kanzureFor the witnessing to be legal the parties generally either have to work for some21:48
kanzurethird party whom they've signed right over to (eg IBM). Or else they have some21:48
kanzureerm.21:48
kanzurehttp://uncyclopedia.wikia.com/wiki/UnNews:Recession_now_affecting_time_itself%3B_one_hour_lost_Sunday21:48
fennsad but true21:51
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fennwow a whole day with no diybio22:26
kanzureI've had no interesting emails this weekend :(22:38
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gene_would you like me to change that kanzure?23:10
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