2009-04-13.log

--- Day changed Mon Apr 13 2009
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kanzurehttp://heybryan.org/books/papers/bibliographies/mania.bib00:31
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kanzureProduction of offspring from a germline stem cell line derived from neonatal ovaries http://heybryan.org/books/papers/Production%20of%20offspring%20from%20a%20germline%20stem%20cell%20line%20derived%20from%20neonatal%20ovaries.pdf00:57
kanzure"00:58
kanzureIt has been controversial whether female germline stem cells (FGSCs) are present in postnatal mammalian ovaries. Cells from a line derived from FGSCs isolated from adult mice that were transplanted into ovaries of infertile animals underwent oogenesis and generated offspring."00:58
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kanzure-argh01:55
kanzure-wtf tito, wtf.01:55
genehackerhuh?01:55
kanzure-so now he's sending emails out to all of the regional diybio lists (but not the main diybio list) trying to set up a new forum for diybio01:55
kanzure-something's seriously wrong with these people01:55
genehackerOH yeah working on writing up a DNA synthesizer project proposal for diybio01:56
kanzure-what type of DNA synthesizer?01:56
genehackerto actually give us some direction other than LET'S MAKE ECOLI THAT GLOWS01:56
kanzure-http://heybryan.org/mediawiki/index.php/DNA_synthesis01:56
genehackermaskless lithography DNA synthesizer01:56
kanzure-how would that work?01:56
genehackerso we can synthesize genomes the size of mycoplasma gentalium 01:57
genehackeror however the fuck it is pronounced01:57
kanzure-so how would you do that?01:58
kanzure-DMD microarrays are not exactly DIY-friendly01:58
genehackerwe don't use DMD microarrays01:58
genehackerwe use a 128x128 black and white lcd screen01:58
kanzure-maybe I'm not seeing how this works01:59
kanzure-those pixels aren't exactly the size of oligonucleotides01:59
kanzure-or nucleotides for that matter01:59
genehackeryeah so?01:59
genehackerI don't think you understand02:00
kanzure-oh, maybe there are certain light-directed DNA synthesis methods02:00
genehackerdo you understand what a photolabile protecting group is?02:00
genehackerthat diagram explains it all02:01
kanzure-phosphoramidite oligonucleotide/DNA synthesis doesn't involve photlabile protecting groups02:01
kanzure-*photolabile02:01
genehackerthat diagram explains it all02:01
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genehackertake off the protecting group put down one nucleotide02:02
genehackernucleotide gets added where photolabile group was removed by light02:02
kanzure-what diagram?02:02
genehackerhttp://heybryan.org/mediawiki/index.php/DNA_synthesis02:02
genehackerthe maskless array diagram02:02
kanzure-oh heh'02:02
genehackerwe can just use an LCD screen from a cellphone02:03
kanzure-so, when you remove a photolabile group via light, how do you make sure that a specific nucleotide is added, rather than one of the other three nucleotides?02:03
genehackera 128x128 display would give us a very long genome02:04
genehackerby adding one at a time kanzure02:04
genehackeradd one wash it off02:04
kanzure-how do you isolate a single nucleotide?02:04
kanzure-oh, okay02:04
kanzure-a wash step is involved?02:04
genehackerput on photolabile in next step02:04
genehackerI think so02:04
genehackerhow else would you do it?02:05
genehackerWhat I want to do though is to figure out how to get the DNA off the chip so you can use overlap extension PCR to compile a genome02:05
genehackeroh OE pcr and annealing02:05
genehackerif we can do this we've done something rather disruptive02:06
genehackerand I'll admit rather scary02:06
genehackerbut scary things are cool02:06
kanzure-so I'm sure you'll like this02:06
kanzure-jonathan cline, don't know if you've met him yet, sent me a paper the other day02:06
kanzure-for microfluidic PCR- there's a lot of microfluidic PCR papers out there02:07
kanzure-but in particular this one was on a rectangular workspace02:07
kanzure-and at the corners of this workspace were heating/cooling elements02:07
kanzure-and in the center was a spiral microchannel02:07
genehackerLINK?02:07
kanzure-so that the reactants travel through the spiral and get heated/cooled in tune to the PCR protocol02:07
kanzure-one moment.02:07
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kanzure-http://heybryan.org/books/papers/microfluidics/gkm389%20Miniaturized%20PCR%20chips%20for%20nucleic%20acid%20amplification%20and%20analysislatest%20advances%20and%20future%20trends.pdf02:08
kanzure-There you go.02:08
genehackeroh PCR02:08
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kanzure-yes02:08
genehackerthat's not as fun or scary as DNA synthesis02:09
kanzure-right02:09
kanzure-oh, btw02:09
genehackerthe cool thing though about having your own DNA synthesizer is that you could use it to test for diseases too02:09
kanzure-there was also a laser/LED-microvalve paper that I found recently.02:09
kanzure-erm, except it was valveless02:10
genehackerthat used UV light to cause some exotic chemical to depolymerize right?02:10
kanzure-something about ultrasonic cavitation and tens of millions of degrees :)02:10
kanzure-no, not an exotic chemical02:10
kanzure-just water, etc.02:10
genehackerhmmm02:10
genehackercool02:11
genehackerUV light and cavitation?02:11
kanzure-I think it was 808 nm, so infrared02:11
genehackerhow many mw?02:12
kanzure-"effectively 700 MW/m^2" or something ridiculous like that02:12
kanzure-one moment02:12
genehackerif 100-200mw you can get a laser like that on ebay02:12
genehackeroh that's not too crazy kanzure02:12
genehackerthat isn't very ridiculous02:12
kanzure-http://heybryan.org/books/papers/Diode%20laser%20generated%20ultrasound%20for%20human%20blood%20cell%20lysis.pdf02:13
kanzure-MW, not mW02:13
genehackerthat's per meter squared02:13
kanzure-yes02:13
genehackerlaser dots can be tiny02:13
kanzure-ref 1 from that paper: http://heybryan.org/books/papers/Whole%20Blood%20Pumped%20by%20Laser%20Driven%20Micropump.pdf02:13
kanzure-808 nm, 500 mW 02:13
genehackersounds like an ebay hair-removal laser should do the trick02:14
genehackerwell I must go02:14
genehackerI've got some work to do02:14
genehackerbeen feeling sick all day, you don't think you could make a sharpie lab on a chip so I can know my enemy?02:15
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genehackerwell AFK02:15
kanzure-sorry, at the moment I don't have a DNA sequencer on a chip.02:16
kanzure-or bacteriophage RNA decoder thingy02:16
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kanzure-say, this doesn't look bad02:31
kanzure-"Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication"02:31
kanzure-"Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes."02:31
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kanzure-heh, PDMS stamping for DNA synthesis02:35
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kanzure-"by rolling the patterned tape past the UV LED, a light-show is played out on the microarray surface in real time"02:55
facefacekanzure-: link?02:58
kanzure-faceface: sorry, that's from my brain03:05
kanzure-doesn't exist yet :)03:05
kanzure-also, what is 3D gel electrophoresis good for?03:05
facefacekanzure-: that is for separating protins... oh wait... 03:06
facefacewhat is the 3rd D?03:06
facefacein 2D its mass then charge (or charge then mass, I forget)03:07
facefacekanzure-: do you have a link for the lazer synthesis of affy chips?03:07
facefaceI heard about it... I'll just google03:07
kanzure-affymatrix chips? no, I don't remember seeing anything like that03:07
facefaceor oligo chips... perhaps it wasn't affy (sorry)03:07
facefacebecause I remember you guys were talking about lazer (wavelength) guided DNA synthesis03:08
kanzure-just uploaded: http://heybryan.org/books/papers/bibliographies/DIY-DNA-synth.bib03:09
kanzure-bibliography of techniques for DNA synthesis that are possibly useful to anybody interested in DIY03:09
facefacewhy is my desktop so broken?03:10
facefacesorry, its just ff03:10
facefacekanzure-: this is what I was on about http://mrw.interscience.wiley.com/emrw/9780471142706/cp/cpnc/article/nc1205/current/abstract03:22
faceface DNA Microarray Preparation by Light-Controlled In Situ Synthesis03:22
facefacerecent ff update... seems it can't load a page and do anything else at teh same time03:24
facefaceff is so crappy03:24
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kanzurefenn: wtf?07:31
kanzurehttp://svn.gna.org/viewcvs/pythonocc/trunk/src/SWIG_src_modular_linux_darwin/?dir_pagestart=5007:31
kanzureJelle claims that the files were in the folder..07:31
kanzureso .. why is the "Make Magazine" logo a blue background with a hammer silhouette? When was the last time you honestly had to use a hammer in any Make Magazine project?07:56
kanzurewhen I was 11 or 12 years old, I used to run a lot of different forums, and later I learned how much of a sign of immaturity it was to run around to different forums advertizing how you're going to split the community and start a new and better forum :/08:05
kanzureespecially just for medium-changes08:05
kanzurebut I'm not sure how to transfer that knowledge to others08:06
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kanzure-http://heybryan.org/books/papers/Controlled%20deposition%20of%20picoliter%20amounts%20of%20fluid%20using%20an%20ultrasonically%20driven%20micropipette.pdf 09:06
kanzure-Controlled deposition of picoliter amounts of fluid using an ultrasonically driven micropipette09:06
kanzure-yay09:07
kanzure-although obvious :)09:07
kanzure-hm, all the way from 2003? "Development of Photolithography System with Liquid Crystal Device as Active Mask for Synthesizing DNA Chips."09:35
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cis-actionhey09:43
kanzure-hey09:47
kanzure-"electro-optical adaptive phase plate" (OALV) <- seems to be the codeword that was being used for that09:47
kanzure-cis-action: what's up?09:47
cis-action1. Nils from Monitor 360 emailed the list a while ago about DNA synthesis info.  Have you chatted with him?09:48
kanzure-Yes, we talked over the phone a few months ago.09:49
kanzure-but I don't remember his email about DNA synthesis09:49
cis-actionHmm, I can't seem to find it.09:50
cis-actionOh well.09:50
cis-action2. I agree in general with you about the forum idea09:51
kanzure-hrm, I wonder why these phase plates are called "light valves"09:52
cis-actionI ended up talking to tito last night.  I think he's starting to feel concerned people who live in areas with regional groups are more likely to post to the regional group instead of the main list.  Somehow making a single "regional group" forum room will solve the problem for him.09:52
kanzure-meh09:53
kanzure-you guys are starting to seem really disorganized09:53
kanzure-people going behind each other's backs etc.09:53
kanzure-just saying :/09:53
cis-action"you guys"09:54
cis-actioninteresting09:55
kanzure-by "you guys" I mean everyone who's not me09:55
kanzure-:p09:55
kanzure-actually I mean everyone who has been suggesting some really bad ideas recently, but whatever, as long as someone other than me is recognizing it09:55
kanzure-"liquid crystal spatial light modulator" hm09:56
kanzure-cis-action: what are you going to do if tito makes the forum or something?09:58
kanzure-just wondering09:58
cis-actionwell, about two weeks ago jason installed vanilla forums at diybio.org/forums09:59
kanzure-right09:59
cis-actionand I guess tito is going to try and convince local conversations to occur there09:59
kanzure-I sent an email suggesting some plugins to install09:59
kanzure-I don't know if anyone read it09:59
kanzure-but basically the summary is that it's a terrible idea, unless you maybe include a mail-to-forum gateway or something09:59
kanzure-so that messages are passed back and forth09:59
kanzure-because you aren't going to get people to migrate from the mailing list to the forums, or something10:00
cis-actionyeah, only like 20-50%?10:00
kanzure-dunno10:01
ybiti can't recall which email and when, but someone on the open manufacturing mentioned "the irc channel", i'm guessing it's this one?10:01
kanzure-ybit: I don't know, I don't remember that individual10:01
kanzure-it was on OM, recently, yes10:01
kanzure-erm. I mean to say I don't know which IRC channel he was referencing because I don't remember him10:01
kanzure-gee, I don't know of any spatial light modulator (SLM) more readily-available than an LCD..10:03
ybit[Open Manufacturing] [OFF-Topic] High-Performance Computation :: from Marcos :: "Hello people, as I may have already mentioned (in the IRC channel, a few months ago)"10:03
kanzure-right10:03
kanzure-I don't remember a Marcos though :/10:03
kanzure-so the LCD methods involve these huge optical lenses that are the size of monolithic desktop towers10:04
kanzure-that's not going to be good..10:04
kanzure-cis-action: so, can you give me any more details on onsingularity for later today? do you know anything about what they might ask?10:05
cis-actionkanzure: aaron emailed us the questions10:06
kanzure-okay, thank you10:06
kanzure-oohh.. optical logic gates via stacking LCDs together10:06
kanzure-oh I see the email10:10
kanzure-excellent set of questions.10:11
cis-actiongreat10:11
cis-actionwell, I'm going to be up and running at 2:00pm at the latest10:11
* kanzure- just updated the DIY-DNA-synth.bib file10:13
kanzure-http://heybryan.org/books/papers/bibliographies/DIY-DNA-synth.bib10:13
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kanzure-in particular I've added some references that might help with DNA synthesis via LCDs10:13
kanzure-(think: cellphones in third world countries?)10:14
cis-actionOk, I've summarized my thoughts re. tito10:14
cis-actionProblem: I think Tito has correctly noticed the beginnings of some regional exclusivity on the lists, (I think the DIYbio-London group alarmed him with their discussions about their Dough project). Conclusion:  I think this is starting to happen because regional lists provide a better sense of community to the local members than does the global diybio list, so over time more and more conversations happen i10:14
cis-actionI'm mostly interested in soliciting the relative experts that exist on iGEM teams - students and PhDs and instructors - and bringing their technical questions out in the open on a mailing list or forum somewhere.  The existing moderated iGEM mailing list - for some reason - does not accomplish this and I'm confident that there has been consistent demand in the iGEM community over the last 5 years for a pla10:15
kanzure-to be honest, I'm okay with regional lists for organizational issues like setting up playdates10:15
kanzure-that seems fine to me10:15
kanzure-cis-action: in your first message, there is cutoff at "and more conservation i----"10:15
kanzure-and in the second, "5 years for a pla-"10:16
cis-actionyeah, but I think there is inevitable social pressure to discuss community matters solely on the list10:16
cis-actionoh10:16
cis-actionok10:16
kanzure-I liked it back when people talked about projects and ideas on the main list10:16
cis-actionhttp://pastebin.com/d61d0771b10:16
kanzure-and then went to their separate mailing lists for organizational issues like who's going to bring the pizza :)10:16
kanzure-I think the main diybio list is an ok place for 'dumb questions'10:17
cis-actionright, but I don't think there is a very strong sense of community on the main list, and hence people start to move their discussion to the regional groups, which somehow do end up with a stronger sense10:17
cis-actionkanzure: I think so too, but see my point re; branding10:17
kanzure-odd, I feel strong community on the main list- what do you mean?10:18
kanzure-so about "experts"10:19
cis-actionkanzure: I think we would be increasing the participation barrier for most igemmers if we had to not only convince them to join an online forum/list, but also explain why it's called diybio10:19
kanzure-well that's the thing10:19
kanzure-these are "experts" in academic labs10:19
kanzure-in their domain they are indeed experts and know how to operate their commercial machinery and so on10:19
cis-action"relative experts"10:19
kanzure-right10:20
kanzure-so relative to what? not really the DIY aspects10:20
kanzure-I agree that it's nice to have these folks around to offer advice and so on10:20
cis-actionthis is The Truth: the average iGEM student knows more and has done more molecular biology than the average DIYbio list member10:20
kanzure-eh, that's arguable.. I mean, they know it within the context of "here's a lab handed to you"10:21
kanzure-but maybe DIYbio will involve different techniques because of the different constraints on acquiring materials, tools etc.?10:21
cis-actionsure they will10:21
kanzure-the end result of diybio isn't necessarily going to look like iGEM10:21
* cis-action agreed10:21
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kanzure-Hi amaruk.10:21
cis-action"I agree that it's nice to have these folks around to offer advice and so on" - they are not around.  I am trying to figure out the best way to incent them to be around.10:22
amarukhi kanzure and all other persons online10:22
kanzure-maybe they just aren't interested in DIY?10:22
kanzure-ok, so let's think of it this way10:23
kanzure-erm, what I mean is that you shouldn't try to force diybio into something that would appeal to those people, even though it's nothing about DIY10:24
cis-actionsure10:25
cis-actionWhat makes you think I'm forcing diybio to be anything at all?10:25
cis-actionI'm talking about something explicitly different from the diybio banner10:25
kanzure-you most commonly post about igem and biobricks more than anything10:25
kanzure-well, what about it?10:26
cis-actionplease see http://pastebin.com/d61d0771b10:26
kanzure-I've read it10:26
kanzure-I don't know what we're talking about10:27
cis-actionok, let's stop then.10:28
kanzure-heh10:28
kanzure-do you think that you would somehow siphon people from an iGem-community-interest community back into diybio?10:28
cis-actionI think there would be some kind of equilibrium between the two, including the possibility of a person being in both groups at once.  But I don't care about that too much - my goal isn't primarily diybio outreach.  It's the development of a place full of technically relevant information and people who know a lot more.10:31
kanzure-may I ask why you have that goal?10:32
kanzure-huh. gelatin has been used for microfabrication via visible color wavelength photolithography10:34
cis-actionBecause I think the primary disadvantage for non-traditional scientists is access, formally and informally, to a group of technically and/or theoretically knowledgeable peers10:36
kanzure-what's the largest LCD on the market in terms of meters?10:45
kanzure-wait. what was that paper about a microlens array for an LED cap?10:48
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kanzure-huh. integration of light source and photomask into a single super-uber-hacked LED10:48
kanzure-"matrix addressable microemitters"10:49
kanzure-oh, wait. two different things there. one is uber-tiny LEDs, the other is the idea of addressing regions of the LED color-cap.10:51
kanzure-I wonder if a drop of water is an effective microlense for microemitter/micro-LEDs10:53
katsmeowglycerin?11:00
kanzure-heh heh11:01
kanzure-looks like I already found a good way to fabricate a microemitter array11:01
kanzure-in /books/papers/microfluidics/ there's a paper about using capillary action to let a polymer go through some microchannels to form a circuit for organic microLEDs11:01
kanzure-but then it uses vacuum deposition for the aluminum electrodes :( don't know how to get around at the moment11:02
katsmeowhmm,, two microgrooved faced plates, face to face at 90 degrees,  they mix and be useable where the channels cross?11:02
kanzure-whaT?11:03
kanzure-imagine a microchannel fabricated in PDMS by pouring around a piece of hair :p and then removing the hair afterwards or something11:03
katsmeowjust thinkingout loud,, i'll think with less typing now....11:03
kanzure-no, it's fine11:03
kanzure-was just wondering what you meant11:03
katsmeowoh, how to get microdots in a grid11:04
kanzure-more or less11:04
kanzure-so what I was thinking about originally was photolithography for DNA synthesis11:04
kanzure-LCDs are one method to do this- but you need some optical lenses to make everything work just right11:04
kanzure-another way to do this would be a micro-array of LEDs11:04
kanzure-so I'm looking into micro-emitter/LED fabrication methods- 11:05
katsmeowif the material in the groove was also conductive, but photily active only where they crossed, and only in one direction, then you get dots of lite, and the grooves themselves are your x-y address lines11:05
kanzure-http://heybryan.org/books/papers/microfluidics/Patterning%20polymer%20LEDs%20by%20micromolding%20in%20capillary%20at%201e-6%20torr.pdf11:05
kanzure-huh? an addressing scheme? but what if you wanted to have many of them on at once?11:06
kanzure-I don't think it would be too hard to come up with an addressing-matrix-circuit-stuff11:06
katsmeowpersiatance of vision11:06
kanzure-I mean, it sucks to design those sorts of circuits, but.11:06
kanzure-oh11:06
katsmeowor phosphors11:06
kanzure-yeah, I guess it doesn't have to be active for too long11:06
kanzure-if there's a ridiculously fast switching time, who cares? :)11:06
kanzure-http://heybryan.org/books/papers/microfluidics/Patterning%20photo-curable%20light-emitting%20organic%20composites%20by%20vertical%20and%20horizontal%20capillarity%20-%20a%20general%20route%20to%20photonic%20nanostructures.pdf11:07
katsmeowerg11:07
kanzure-ugauga?11:08
katsmeownah, can't get the flash videos from http://blog.wired.com/wiredscience/2009/04/yourfriendatom.html11:08
kanzure-but that's because flash sucks11:08
* katsmeow nods11:09
kanzure-any ideas on a completely polymer-based LED? or something11:09
katsmeowmost humans online now don't *remember* the civilian uses proposed for nukes back then, and don't have the same sense of horror at the thought they'd actually go thru with them11:09
kanzure-because that micromolding paper is great, but the electrodes are placed by vacuum deposition11:09
katsmeowno, other than make it possible before we run out of the raw materials for making such11:10
kanzure-is Project Orion (atom bomb propulsion) civilian?11:10
katsmeowyeas,, well, NASA11:10
katsmeowthe *current* Orion project is not nuke-based, it's just huge11:10
katsmeowthe *current* Orion is a oversides Apollo 11:11
kanzure-yeah I know11:12
katsmeowthere is still some thought tomerging the *old* Orio with the big gun approach, so the nuke fire underground to acchieve escape velocity at the surface,, still no way to prevent fissile product emmisions11:12
kanzure-haven't they ever heard of namespace conflicts11:12
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kanzure-http://www.engadget.com/2007/01/08/sonys-1-000-000-1-contrast-ratio-27-inch-oled-hdtv/11:19
katsmeowi hear the blue in oled still fades out in a few yrs?11:21
kanzure-hm. what's the problem with OLEDs again?11:24
kanzure-there was some particular reason why they weren't catching on or something11:25
kanzure-I want to be sure me trying to manufacture OLEDs isn't going to be some silly-impossible Don Quixote quest11:25
kanzure-"Indium tin oxide is commonly used as the anode material. It is transparent to visible light and has a high work function which promotes injection of holes into the polymer layer. Metals such as aluminium and calcium are often used for the cathode as they have low work functions which promote injection of electrons into the polymer layer."11:26
kanzure-"The production of small-molecule displays often involves vacuum deposition, which makes the production process more expensive than other processing techniques (see below)."11:27
katsmeowIndium was one of the materials suggested to run out with mass production of silicon pv panels11:27
kanzure-"Recently a hybrid light-emitting layer has been developed that uses nonconductive polymers doped with light-emitting, conductive molecules. The polymer is used for its production and mechanical advantages without worrying about optical properties. The small molecules then emit the light and have the same longevity that they have in the SM-OLEDs."11:27
kanzure-oh yay. that doesn't sound good at all, katsmeow 11:27
* katsmeow nods11:27
kanzure-"electroluminscent conductive polymer"11:28
kanzure-hm, polyfluorene11:28
kanzure-or light-emitting polymers11:29
kanzure-can anyone get me this paper? "Ink-jet-printable phosphorescent organic light-emitting-diode devices"11:33
kanzure-http://dx.doi.org/10.1889/JSID16.12.122911:33
katsmeowcan you register the led over the previously printed electronics with a printhead, at a interestingly small scale?11:34
kanzure-that would be neat.11:38
kanzure-but first I need to get the paper :p11:38
katsmeowdamned forking nosey .gov sites,, no way to dl the pdf on http://www.osti.gov/bridge/purl.cover.jsp;jsessionid=14FFB880A12A936C587B0CD04CAC717F?purl=/408695-xwEMy7/webviewable/  without making up a new http header that tells them what they want to know, and i dunno what it is they want to know11:45
kanzure-ok, here's a late 90s paper for 250 micron diameter OLEDs11:46
kanzure-using PVK, indium tin oxide, and a reflecting cathode11:47
kanzure-hrm. still using indium tin oxide. blah.11:47
facefacekanzure-: that DOI url just says 'OK' in links11:55
facefaceInk-jet-printable phosphorescent organic light-emitting-diode devices - what is the pdf called11:56
faceface?11:56
kanzure-I don't know what the PDF is called :(11:58
kanzure-that's the title of the paper11:58
katsmeowso i switched to IE to dl that pdf from the .gov site, and locked up IE AND firefox AND the desktop display11:58
facefaceI just moved all my pdfs...11:58
facefacenothing new turns up11:58
kanzure-katsmeow: need some help?11:58
kanzure-faceface: what?11:58
katsmeowi taskmaster'd the IE session to death, i'm ok11:59
facefacelynx here says 'buy the pdf', links from an inst just says 'OK' I wondered if a pdf had appeared11:59
facefacewget on the DOI does weird things..11:59
kanzure-faceface: yeah, that's why I can't find the PDF :)12:00
katsmeowyeas, wget doesn't half work anyhow, --keep-session-cookies isn't defined anywhere cept the help file :-/12:00
katsmeowneither does  restrict directories12:01
katsmeowi run wget thru a proxy i can better configure12:01
katsmeowthat way i can change things as wget is running  too :-)12:01
fennindium is not going to run out12:01
katsmeowit was either indiumor iridium12:01
fennit's the same idiocy that's been applied to uranium by peak oil fearmongers12:02
katsmeowi believe it was indium12:02
katsmeowoh, uranium prolly won't run out, it's just the chinese will have bought it all up12:02
fennZOMG we only have 4000 tons of Indium in stock!12:02
katsmeowit wasn't the zero-point running-out that was the problem, it was price increases due to *thinking* it was reare, witness 2008 $150/barrel oil12:03
facefacecan't find a url12:03
fennwell that's even worse to fearmonger about then12:04
kanzure-faceface: did you see the AIP URL?12:04
facefaceno12:04
kanzure-faceface: http://scitation.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=JSIDE8000016000012001229000001&idtype=cvips&gifs=yes12:04
facefaceNo results found for "Ink-jet-printablephosphorescent organic light-emitting-diode devices".12:04
kanzure-check that link :)12:04
facefacelinks just comes up 'OK'12:05
facefaceno idea what that means!12:05
kanzure-hrm12:05
kanzure-what about this? http://dx.doi.org/10.1889/JSID16.12.122912:05
facefacesame12:06
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kanzure-guess you don't have access ..12:06
kanzure-it loads up the abstract for me12:06
facefaceok, you got it now?12:07
kanzure-no12:07
kanzure-I need the paper, not the abstract12:07
faceface/getpdf/servlet/GetPDFServlet?filetype=pdf&id=JSIDE80000160000120012:07
faceface1229000001&idtype=cvips&prog=normal12:08
facefacewhere is that relative to do you think?12:08
kanzure-dunno :(12:08
facefacei.e. where does the doi send you?12:08
facefacehttp://scitation.aip.org/ ?12:09
facefacelemmy try12:09
kanzure-yeah12:09
facefacetakes me to a login page12:10
kanzure-hrm..12:10
faceface'if you would like to purchase this article...'12:10
faceface15 dollar12:10
facefaceI'll write and tell them my mom only lets me spend 1012:10
nshemail the author12:11
kanzure-gasp! social communication?!12:11
nshyou do far more of it than i :-)12:11
facefaceJ. Soc. Inf. Display 16, 1229 (2008)12:11
kanzure-nsh: doesn't count, you don't complain about me yapping all the time12:12
* faceface tries the live chat link through links12:12
facefacewhy not chat and say... oh I baught this liek one month agoo maaan12:13
facefacefail12:13
facefacewell.. this is as close as I can get "http://scitation.aip.org//getpdf/servlet/GetPDFServlet?filetype=pdf&id=JSIDE8000016000012001229000001&idtype=cvips&prog=normal" that link may be usefull for someone at a different institute12:14
facefacenight12:14
kanzure-night12:17
kanzure-updated DIY-DNA-synth.bib again12:18
fenngiven $20/4GB RAM, $25 for 16GB SD card, and $125 for a 30GB SSD, um, why am I supposed to buy the SSD?12:24
kanzure-maybe there's a way to use LCDs without having to use the nasty optics/lense12:24
kanzure-why haven't we seen anybody printing sheets of LEDs with inkjets showing up in Make Magazine and that circle in general?12:25
kanzure-don't these people love LEDs?12:25
fennbecause they 1) dont know how to hack an inkjet and 2) don't have the nasty chemicals necessary12:26
kanzure-that's what I keep forgetting about these polymer techs12:26
kanzure-'nasty' polymers. hrm.12:26
kanzure-should I avoid them?12:26
fennthe LED paper was inorganic12:27
kanzure-did you see the micromolding one? that was an organic polymer IIRC12:27
fennmost semiconductor dopants are toxic heavy metals12:27
kanzure-what about PDOF, PPV, or MEH-PPV? 12:28
fenni dont even know what those are12:29
kanzure-polymers12:29
kanzure-okay, nevermind12:29
kanzure-if it's not something you know it's probably not all that common12:29
fenndo you know any organic chemists?12:31
fennheh never thought i'd have trouble looking for an organic chemist12:32
fenn(both parents taught in organic chemistry dept)12:32
kanzure-treadwell12:33
kanzure-oh, so your parents got it on? to the fumes of nasty organic solvents? hehe12:33
fennyeah and i turned out fine, right? :\12:34
kanzure-yes?12:34
fennactually they spent more time in offices/computer lab than a real lab12:35
fennthe place still smelled weird though12:36
kanzure-hah12:36
fennkind of a fresh paint smell everywhere12:36
kanzure-hm. now I'm worried.12:39
kanzure-Hong H Lee / Kuhp have shown up again in my search results12:39
kanzure-this time with a way of doing cathode fabrication via pressing12:39
kanzure-instead of a ridiculously high vacuum12:40
kanzure-which makes sense, this is what some of their other research was working with12:40
kanzure-but it's like an exact solution to my problem :p12:40
kanzure-20x10 passive OLED matrix12:41
fennpressing metal foils?12:41
fennor PDMS printing stuff12:41
fennwhat does 'passive' mean?12:42
kanzure-dunno. but there are active/passive LCDs apparently12:43
fennthey're not LCD's12:43
kanzure-um, so apparently they had a protruding pattern on a glass surface12:43
kanzure-right, but I think it's the same wording12:43
kanzure-so then they treat that glass surface with a "self-assembled monolayer (SAM) material, which is followed by the deposition of the cathode materila, such as aluminum, onto the whole surface"12:44
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fennIn a passive matrix OLED (PMOLED) display, the diodes are connected in a grid, each diode comprising an individual OLED pixel. The rows of the grid are lit one at a time using external drive circuitry. In contrast, active matrix displays include transistors within the display enabling pixels to be continuously illuminated.12:44
kanzure-so it relies on poor adhesion strength between the metal and SAM12:45
kanzure-and a greater adhesion strength between metal and the polymer12:45
fennso LCD's have transistors in them already?12:45
kanzure-I heard something like that once, yes12:45
fennhum i guess that's what TFT means (thin film transistor)12:45
kanzure-ack, so many nasty chemicals12:47
fennhow the hell do they make a fet out of polycrystalline silicon12:49
kanzure-is that the gap?12:50
fennwhat gap?12:50
kanzure-don't FETs have a gap over which the field electric effect performs?12:51
fennthe gate insulator?12:51
kanzure-blah12:51
fenni'd presume the gate is made from ITO or silver12:52
kanzure-so: make a microfluidic circuit that a polymer can capillary-action itself through; this circuit would be the OLED array device thingy; then use cathode contact/pressing to add in the metal cathodes. sounds simple in principle ..12:56
fennyeah, sure12:57
kanzure-meh.. maybe it'd just be easier to go get that lense to make the LCD method work. /me will need to go look that up12:58
fennwhat's the problem with the lens?12:59
fennyou know about water lenses right?12:59
kanzure-it's huge.12:59
kanzure-http://heybryan.org/books/papers/microfluidics/Versatile%20stepper%20based%20maskless%20microlithography%20using%20a%20liquid%20crystal%20display%20for%20direct%20write%20of%20binary%20and%20multilevel%20microstructures.pdf13:00
kanzure-pg 313:00
fennyou know your server's upload has been like 10kb for weeks right?13:00
kanzure-um. no?13:01
kanzure-ybit: are you still raping my server?13:01
kanzure-anyway, it's already on the other servers13:02
kanzure-http://sata.serveftp.org/~bryan/papers/microfluidics/Versatile%20stepper%20based%20maskless%20microlithography%20using%20a%20liquid%20crystal%20display%20for%20direct%20write%20of%20binary%20and%20multilevel%20microstructures.pdf13:02
fennok, so use a smaller panel13:05
kanzure-I don't know if that would help.13:06
kanzure-what's the condenser for, anyway?13:07
kanzure-oh, reduction lens13:07
kanzure-who cares then?13:07
kanzure-as long as you have something like 100x100 pixels (for starters) that's good enough for a reasonably sized array13:07
fennthe condenser is to collimate the lamp output13:08
fennfwiw you could probably use an overhead projector13:10
kanzure-ok. now to figure out photolithography methods of dna synthesis13:11
kanzure-before I do something evil (and boot into Windows), do you know of any good webcam software?13:11
fennfor doing what13:12
fenni've used xawtv but i wouldnt call it 'good'13:12
kanzure-conferencing with onsingularity and mac13:12
fennekiga is supposed to work..13:12
kanzure-huh, tokbox.com actually worked (except for the microphone..)13:19
cis-actionhey13:19
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bkeroDo any of you remember that 1950s spaceman facebook/twitter/whatever pic that someone posted?13:42
bkeroAhh, yes.  http://2.bp.blogspot.com/_b7Sh_4xAn1I/SabQN3uuX6I/AAAAAAAAAxs/928iWGi7nt8/s1600-h/24w7ed0.jpg13:42
kanzurecis-action: I'm so confused about what just happened14:03
kanzurewas that because of my connection or because of their servers?14:03
cis-actionwho knows14:08
cis-actionthere were several bad factors14:09
cis-actionall of our audio was bad - but your audio was particularly bad14:09
kanzureheh okay14:11
kanzurecis-action: just sent out an email about DIY DNA synthesis again14:12
kanzurehope you like :)14:12
kanzureyay for getarticles, they got my paper :)14:14
cis-actionseriously14:15
kanzurehm?14:15
cis-actiongetarticles is great14:16
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kanzurehah14:58
kanzurecan't believe I missed this14:58
kanzurehttp://heybryan.org/mediawiki/index.php/How_bryan_wastes_his_time14:58
kanzure(see the line that reads 'edit')14:58
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genehackermicrolens are easy14:59
kanzuregenehacker: fenn suggests they aren't needed for the LCD15:00
kanzurealso15:00
kanzurehttp://heybryan.org/mediawiki/index.php/AFM_nanolithography15:00
kanzureI totally forgot that I wrote that page15:00
genehackerthere is a professor here who does microlenses15:00
genehackerone paper I saw on making microlenses used superglue and a 2d printer to make them15:00
kanzuredo you need microlenses?15:03
genehackerno15:08
cis-actionbrian, why won't dlp chips work for diy synthesis?15:09
kanzurecis-action: the DMD mirrors chips?15:11
kanzurecis-action: those microarrays of mirrors cost a lot of money, and they are not easy to make.15:12
genehackerthey will work15:14
genehackerthey are just expensive15:14
genehackerbut DLP chips are getting cheaper...15:14
genehackersoon there gonna be in phones15:15
genehackerso you can project the phones screen onto a large surface15:15
genehackeror have your phone display holograms if you get 2 dmd chips15:16
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kanzureHey splicer.15:25
splicerhi kanzure15:27
kanzurehave you been keeping up with my posts to diybio? what do you think of things?15:32
kanzurefenn: you and les might have some contact from a GLXP team that I'm on re: manufacturing of aerospace components15:33
splicerkanzure: haven't read diybio in 2 weeks... going to look at it now15:35
kanzureheh they have funds15:36
fennthe sideways hot air gun people?15:36
kanzureinterplanetary ventures15:37
kanzurehttp://www.interplanetaryventures.org/15:37
kanzuredave wanted his aerospace contracts, right?15:38
fennhm. dave is an idiot15:39
kanzureeh?15:39
kanzureaerospace contracts don't make one an idiot15:39
fennit takes more than a CNC machine to do high class work15:40
kanzureheh, "magic super axis CNC machine! go!"15:40
fennanyway what did interplanetary want?15:42
kanzureone moment, I'm dragging the guy in here15:42
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kanzureHello.15:43
Hell_Tangohello15:43
-!- Hell_Tango is now known as Sean15:44
kanzureso everyone else in here is unfamiliar with IVP15:44
kanzurebut is interested in the potential interaction with a fablab etc.15:44
Seani am sean aka helltango15:44
SeanIPV15:44
-!- Sean is now known as Guest5925015:44
kanzureyes, IPV15:44
Guest59250not IVP15:44
kanzuresorry15:44
-!- Guest59250 is now known as Sean_con15:44
Sean_connever mind15:44
Sean_conIPV or interplanetary ventures is a open source team , for space and planetary exploration and development15:45
Sean_conthe CEO is kevin e myrick15:45
Sean_conthe webpage is http://www.interplanetaryventures.org15:46
Sean_conas our latest project, we are competing in google lunar x prize15:47
Sean_consponsored by 15:47
Sean_conInterorbital systems15:47
Sean_conand15:47
Sean_conespacetickets15:47
Sean_conhowever, we are still looking for participants together with whom we can develop our systems15:48
kanzureso I mentioned a fablab project. I guess I haven't gone over open source manufacturing yet either or what we're doing in here15:49
kanzurebasically if there's a chance that we can help each other out, that would be worth exploring15:50
Sean_coni guess there is15:50
Sean_conWe are interested in collab15:50
Sean_conplease ask me questions, if you are interested15:51
kanzurewell I was hoping to poke fenn a bit15:51
kanzuresince he's been paying more attention to the actual place than I have15:52
Sean_conok15:52
Sean_coni fenn15:52
splicerkanzure: on diybio; i don't really have opinions on any goings on in the last 2 weeks there. 15:52
kanzuresplicer: come on, surely the DIY DNA synthesis is interesting :)15:52
splicerif it works15:52
kanzurewell, no, not yet15:53
spliceryou're right... it can be cool some day15:53
* katsmeow-afk listens to pins drop15:55
kanzurehm?15:55
kanzurewell I was hoping to make fenn somewhat social but maybe that won't happen15:55
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kanzureSean_con: you might be interested in reading up on some of the projects that we are related to in here15:56
kanzurehttp://heybryan.org/om.html15:56
kanzurein particular the 2nd link for instance15:56
kanzurewe're working on an open source hardware package management system, somewhat like "apt-get" or "yum" or "yast" or rpm's except for hardware,15:56
kanzureand ideally integrating it with a fabrication facility for further F/OSH projects.15:57
splicerkanz: ....I have to be antisocial too, have some stuff I have to get done before morning15:57
Sean_conthere is no hurry, so no worries15:57
Sean_conyah, i see them15:57
kanzureokay15:57
Sean_conthe autonomous exploration is closest to what i am up to15:58
Sean_confrom IPV side, i would say, it is possible to collab15:59
Sean_conif you want, i can send a formal collab letter along15:59
kanzureso you mentioned asteroid mining15:59
kanzuredo you know various path finding/landing algorithms?15:59
fennuh, hi. sorry i was distracted by diybio16:00
Sean_conyah16:00
kanzurefenn: heh I already replied to you16:00
fennyou write too much :{16:00
kanzureit's true.16:00
Sean_coni am working on currently more than one open source path fining algorithms for autonomous vehicles16:01
kanzureanything in particular?16:02
Sean_confor instance, autonomous vehicle , guided by it's camera to explore underwater reefs16:02
Sean_conit must not collide with the reef in any chance16:02
kanzureso, OpenCV?16:02
Sean_conopenCV? sorry i do not know the term16:03
kanzureit's an open source visual computation package for problems just like that16:03
fenni'm curious how you define 'open source space development' exactly16:03
fennfor example, do you share your CAD files?16:04
fennwhat prevents others from advancing your work and then not releasing the results?16:04
Sean_condo you share your CAD files? - yes16:04
Sean_conwhat prevents others from advancing your work and then not releasing the results? - IDK, maybe they are lazy16:05
kanzureheh16:05
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fennseems to me laziness tends to result in people not sharing, because it's too much bother to post something online16:06
Sean_conopen source visual computation package for problems just like that - we want our truly customizable package - a couple of Fourier based methods have been tested with partial success only 16:06
fennbut i'm more concerned about patent trolls and unscrupulous chinese factory owners16:06
Sean_conbecause there is an acute lack of easily recognizable landmark in underwater topography16:07
Sean_conunscrupulous chinese factory owners - i am too16:07
Sean_conso we for example, we release the complete cad files ( available from boris or kevin), but they are marked with -@ intellectual property of ...' stamps16:08
Sean_conso we for example, we release the complete cad files ( available from boris or kevin), but they are marked with - 'intellectual property of ...' stamps16:08
fennuh, 'intellectual property' is a fictional concept16:09
fennthe only things that are recognized by the law are copyright, patent, and trademark16:09
fennalso there are nondisclosure agreements, but that's part of contract law16:09
fennasking someone to sign a nondisclosure agreement could theoretically lead to some sort of copyleft for hardware, but it seems difficult16:10
fennbleh anyway16:10
fenni've stayed away from space hardware development because the minimum scale is too large for one person to make a dent16:11
fennat least i don't consider suborbital to be worth doing16:11
fennthere really needs to be an unmanned space station in a reasonable orbit16:12
Sean_conwe know16:12
fennnot government owned16:12
Sean_conwe are targeting that16:12
fenncommodity electronics, communications and so on16:13
Sean_conbut we need developers, who can stick to us, till we achive that stage16:13
Sean_conbecause the minimum scale is too large for one person to make a dent - same here, seeking help16:13
fennyour page has pictures of nasa and russian vehicles.. what are you working on?16:13
Sean_conspace and planetary exploration and development16:14
fenn"photos, videos, and other materials are available upon request" isn't very open source16:14
Sean_conwe do not yet have privately funded missions on moon16:15
Sean_conno, that is to save bandwidth actually16:15
Sean_con;-(16:15
fennthat's a lame excuse16:15
Sean_conwell, it's not16:15
fennflickr, youtube, what's the problem16:15
Sean_conwe have youtube and flicker channels16:17
Sean_conwait16:17
Sean_coni give you links16:17
wrldpc..16:17
Sean_conhttp://www.youtube.com/watch?v=pOeJ1jRKbZw&feature=channel_page16:18
Sean_coni agree that we are not totally professionals, in the sense, media is kind of disorganized16:21
fennyour bootstrap plan has way too much reliance on humans16:25
Sean_conyes16:25
fennand you launch everything from the ground.. which costs too much16:26
fennif an international consortium of governments doesn't have the budget, you sure won't16:26
Sean_conif an international consortium of governments doesn't have the budget, you sure won't16:27
fennfocus on business plans; what are you going to do in space once you're there?16:27
Sean_conand you launch everything from the ground.. which costs too much - no16:27
fennif you dont know, nobody's going to give you money to develop the concept16:27
Sean_confocus on business plans; what are you going to do in space once you're there? - develop industry16:27
fennwhat sort of industry16:28
Sean_conmine asteroids16:28
Sean_concolonize them16:28
Sean_conmine moon16:28
Sean_conwe had to start somewhere16:28
Sean_conand we started16:28
kanzureI thought you said you already had funding16:28
fennalright, well.. you need more detail16:28
Sean_conyes16:30
fennx-plane only simulates a tiny fraction of what you need to consider16:30
Sean_conwe do have funding16:30
Sean_conyes16:30
kanzureO.o16:30
Sean_conthats why we are looking  for developers16:30
fennhonestly i dont see what you're offering that would entice someone to join16:31
Sean_conwhat you are looking for?16:32
fennwell, either an established (small?) business with extensive contacts with paying clients, an engineer with some solid engineering skills, or an entrepreneur who is good at convincing rich people to throw huge wads of money their way16:33
fennartemis project at least has a good vision16:34
Sean_coneither an established (small?) business with extensive contacts with paying clients - we are a non profit org16:34
Sean_conan engineer with some solid engineering skills - look at the team portofolio16:34
Sean_conor an entrepreneur who is good at convincing rich people to throw huge wads of money their way - look here: http://www.synergymoon.org16:35
fennfrom your 'about us' page it seems there arent even any technical people?16:35
Sean_condefine technical16:36
fennok i was looking at interplanetaryventures.org16:37
fennwhich has 'communications and student activities'16:37
fennso now i'm really confused - what is the relationship between IPV and synergymoon?16:38
Sean_consynergymoon is a collab project of ipv16:38
ybitkanzure: hah. i've raped it several times over the past month, but not this past week. it's getting all the papers, and everything on the wiki16:39
Sean_concollab: IPV, IOS, HSP16:40
kanzureybit: I installed a module in mediawiki to export everything to xml .. maybe you would like to use that16:41
fenni like the sound of this "The IOS rocket systems have been streamlined to the point that the only moving parts they employ are valves."16:46
Sean_con:D16:47
Sean_conso what are you thinking now?16:50
fenni am thinking you guys have no clue what you're doing :P16:51
Sean_conwhy do you have such an idea?16:51
fennoh it's just a feeling16:51
fennIOS seems to have their shit together16:51
Sean_conwe know we are aiming high16:51
Sean_conyes16:51
Sean_conwe are looking for help 16:54
genehackerIOS rocket system sounds like fluidics are involved, are they?16:54
fenndepends how you define fluidics i guess16:55
genehackerfluidics don't have moving parts16:55
Sean_conright16:55
Sean_conyah16:55
genehackervalves aren't fluidic16:55
fenngenehacker: i think they are just pressure fed big dumb boosters16:56
genehackerhey fenn, I got a couple papers on fluidic rocket control systems16:57
genehackerlink to more info on this rocket?16:57
fennhttp://www.interorbital.com/Sea%20Star%20OLV%20Page_1.htm16:58
genehackerwhite fuming nitric acid?16:59
fennthere should be some law that aerospace companies have to define all acronyms at the start of the document16:59
genehackerthey tried rockets that used pink fuming nitric acid and anilene as a propellent17:00
genehackerbut when things went wrong, the rocket would put out a pink and corrosive "cloud of death"17:01
fennmasten space is still in business.. that's good to hear17:01
fennused to chat with their CTO17:02
fennMichael Mealling17:02
fennSean_con: look at masten-space.com for example of how to put on a good show17:03
genehackersigh, I forgot about this one aerospace company trying to sell heavy lift rockets or something like that a while back17:04
fennthey have big trucks and test vehicles now, but it wasn't always that way17:04
genehackerthat got put out of business by the government17:04
fenngenehacker: the carbon fiber rockets? the guy was a banker right?17:05
genehackerdon't know17:05
Sean_confenn17:05
Sean_coni am not the show man17:05
genehackerdo you know what the company is called?17:05
Sean_conbut i will forward it to the people17:05
fennit was named after the guy.. D... Aerospace17:07
fennBeal Aerospace17:09
fenn"NASA has changed the evolutionary process for new companies and tilted the playing field against private efforts.  As a result there is no role for new launch service companies except as commodity subcontractors to NASA and its primes."17:09
Sean_con>"NASA has changed the evolutionary process for new companies and tilted the playing field against private efforts. As a result there is no role for new launch service companies except as commodity subcontractors to NASA and its primes." the challenge is to emerge as a private group17:11
genehackeryeah that's them17:13
fennwell i could think of worse ways to waste 250 million dollars17:15
fennbut jeez don't just dump it into the river17:15
Sean_con?17:18
fennthey abandoned the project and scrapped all the development equipment and prototypes17:19
Sean_conic17:20
Sean_conanyway, we started this discussion with the idea of a collab17:20
Sean_conyour fablab needs support like finance etc17:29
Sean_conwe need people to help development17:29
kanzurefenn: I thought the CEO of Masten Space was David Masten.17:29
fennsorry, CFO, not CTO17:30
fennall those letters, you know17:30
kanzuregasp! letters!17:30
fennacronym expansion considered harmful17:30
* kanzure is still spazzing out. thinks his doctor prescribed him something bad.17:30
kanzureI don't think I've stopped typing for the last 24 hours17:30
kanzureis this bad?17:31
genehackerdid you go to class?17:31
kanzureclass?17:31
kanzureno17:31
kanzurethis semester "doesn't count"17:31
genehackeryes it is bad17:32
kanzureoh.17:44
nshmeh17:56
nshare you being productive?17:56
nshif so, don't worry17:56
nshyour doctor gave you drugs that made you productive for 24 hours. this is not something to worry about. this is something to replicate17:57
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kanzureoh18:34
nsh(theaboveisnottobeconstruedasmedicaladvice)18:52
fenn(old but better than the nothing they provide now) opencascade documentation  http://myweb.tiscali.co.uk/dolbey/QtOpenCascade/doc5.1.zip19:34
fenni assume there's some windows program that opens that .HLP file19:43
fennwild magic is lgpl now?19:49
kanzurefenn: there's something through wine that runs .hlp files and .chm files or something19:59
kanzureor actually there's a KDE app that does it19:59
fennKchmViewer, why didnt i think of that!~20:03
kanzurebecause you're not using debtags?20:04
kanzure:p20:04
fennyeah, because "works-with::text" would have helped find it20:04
fenni wish some huge company would send me to an opencascade training seminar20:09
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fennthis help file sure beats nothing though20:13
kanzure-wasn't there something for 6.3?20:18
kanzure-I was just trying to get around with help(stuff) in python :/20:18
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kanzure-neat, my server shows up when searching for "Remote Excitation of Neuronal Circuits Using Low-Intensity, Low-Frequency Ultrasound20:22
fennopenSBP is a bunch of shit, like i thought20:37
kanzure-anybody doing brain stem cell therapy would be silly not to include channelrhodopsin-2 genes .. it's like pure win.20:49
kanzure-neat, reprogramming of any mouse cell into iPS cells20:51
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fennisnt that old news? thought they used human skin cells like 6 months ago21:02
fenni'd love to see a fully hyperlinked version of this article http://en.wikipedia.org/wiki/Induced_pluripotent_stem_cell21:07
kanzure-that was Yamanaka and yes21:07
kanzure-blah21:07
* kanzure- will get right on it21:07
kanzure-but in particular I misread21:07
kanzure-this paper is about using mRNAs to reprogram adult cells into embryonic stem cells21:07
fenni mean there's all these gene names with mysterious acronyms21:07
kanzure-black magic!21:07
kanzure-I wonder where I put my stem cell paper collection21:08
kanzure-behold ! http://heybryan.org/books/Biology/stemcells/21:08
fennmRNA seems like the right approach, rather than viruses21:09
* kanzure- uploads stemcells.bib21:09
fennis it virus-free induction blah blah..pdf?21:10
kanzure-no, sorry, one moment21:10
fenngood thing they finally lifted that ban eh?~~21:11
fennjust in time for it to not matter21:11
kanzure-just uploaded21:12
kanzure-it's the one with 'microRNAs' in the title21:13
fenni find it interesting they actually call it 'reprogramming'21:13
kanzure-somebody doing something right?21:13
fennit just seems like they would mumbo-jumbo it up with "induced expression factors" or some crap21:14
kanzure-oh, they do- look at the fourth line of the abstract21:14
kanzure-at least I recognize some of the gene names on the fifth line21:14
kanzure-god I must be sick21:14
fennwrong planet21:15
kanzure-they forgot to give me a map21:15
fennand an operator's manual21:15
fennand a money-back guarantee or at least a manufacturer's warranty21:16
kanzure-eh just take these pills until your mom is happy with you21:16
kanzure-a manufacturer's warranty would be neat21:16
kanzure-oops, I meant miRNAs, not mRNAs21:17
fenni dont know what the difference is21:17
fennshouldn't that be uRNA? :)21:18
kanzure-mRNAs are messenger RNA molecules21:18
kanzure-microRNA is another structure apparently21:18
kanzure-you mean mu RNA21:18
kanzure-or I guess {\mu} if you want to be all LaTeXy about it21:18
kanzure-I think I've learned a sizable subset of LaTeX just by reading misprints in titles on journal websites that forgot to convert titles into unicode text21:19
fenngod can't they just stick all this on a plasma and send in some agrobacterium21:19
kanzure-a plasma?21:19
fennplasmid*21:19
kanzure-how would that do anything long-term?21:20
kanzure-I guess for miRNAs, you mean?21:20
kanzure-for miRNA delivery21:20
fennright21:20
fennand skip all the intron spliceosome crap21:20
fenni hate being an ugly hack21:21
kanzure-wah, so do something about it21:23
kanzure-or not21:24
fennthat's going to take a while21:24
kanzure-so, as I was saying earlier21:27
kanzure-there are apparently certain places in the world giving stem cell treatments for brain abnormalities21:28
kanzure-i.e., "here you go, an injection of embryonic stem cells for your brains"21:28
kanzure-if anything, I would opt to get those optogenetic neurons21:28
kanzure-the ones with photo-activated ion channels in the membrane21:28
kanzure-for laser-induced activation of regions of the brain :)21:29
kanzure-heh, I wonder if you could cell your stem cells as brain implants21:32
kanzure-s/cell/sell/21:32
kanzure-(note I'm not using /g so neigh)21:32
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fenni love those green glowing embryo pictures21:35
fennhuh did you know they made a recombinant human embryo at cornell in march?21:41
fennwow this guy comes right out and says it21:42
fenn"abortion and euthanasia are both fine, desirable even; parents should be allowed to create designer or cloned babies; there's nothing wrong with a drug-fuelled Olympics; scientists and medics should strive to make us immortal, even on a crowded planet; our bodies should be routinely plundered after death for organs, even if the dead and bereaved do not wish it; it is morally justified to compel people to participate in scientific tri21:42
kanzure-cutoff at "participate in scientific tri--"21:42
fenntrials, just as we compel them to do jury service21:42
kanzure-so, alcor's out of the picture?21:43
fenni guess that goes under 'strive to make us immortal' somewhere21:43
katsmeow-afkfenn, url?21:50
fennbah, right after i closed it21:50
fennhttp://www.dailygalaxy.com/my_weblog/2009/03/the-worlds-fi-1.html21:50
katsmeow-afkthanx :-)21:50
katsmeow-afkEthicists, however, warn that genetically modifying embryos will lead to designer babies preloaded with socially desirable traits involving height, intelligence and coloring."  <<== i believe humans should be 4ft tall max, have a 260 IQ, and be varigated purple stripes with yellow pokadots21:57
kanzure-these people are idiots21:58
kanzure-why do they get to decide what they think everyone else is desirable21:59
kanzure-uh21:59
kanzure-or however you make that make sense21:59
kanzure-the point is, they are idiots21:59
genehackerhey why not have a kid with purple strips22:00
genehackerit can be done22:01
fenngenehacker: because god hates purple stripes, that's why22:01
fennmay a pox fall upon thee22:01
fennand may you lose all your funding22:01
katsmeow-afkwell, the 4ft tall max and the 260 IQ is prolly a good idea22:04
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kanzure-in vitro multi-generational embryo selection. something to put on the todo list.22:06
kanzure-especially with that recent oogenesis paper 22:06
kanzure-http://heybryan.org/books/papers/Production%20of%20offspring%20from%20a%20germline%20stem%20cell%20line%20derived%20from%20neonatal%20ovaries.pdf22:07
katsmeowwould those immature eggs be rightly called stems cells?22:08
kanzure-I thought oocytes weren't stem cells.22:13
katsmeowi agree22:15
katsmeowhttp://www.dailygalaxy.com/my_weblog/2007/08/mind-children-t.htm  is not there?22:16
katsmeow+++GET 167980+++22:16
katsmeowGET /my_weblog/2007/08/mind-children-t.htm HTTP/1.122:16
katsmeowHost: www.dailygalaxy.com22:16
katsmeow+++RESP 167980+++22:16
katsmeowHTTP/1.1 404 Not Found22:16
katsmeowthe link is on http://www.dailygalaxy.com/my_weblog/2009/03/the-worlds-fi-1.html22:17
kanzure-Are you wget-only?22:17
katsmeowfirefox22:17
kanzure-they are idiots22:17
kanzure-2~http://www.dailygalaxy.com/my_weblog/2007/08/mind-children-t.html22:17
kanzure-try that22:17
kanzure-they thought they had a .htm file instead of .html22:17
kanzure-ugh22:18
kanzure-this hurts22:18
katsmeowoh, heh, they linked badly22:18
kanzure-"will inevitably lead to inequality"22:18
kanzure-bullfuckingshit22:18
genehackerhttp://www.geocities.com/sumanchakraborty_iitkgp/research.htm22:18
kanzure-fine, go ahead and ignore 40 years of Freitas22:18
genehackeroptofluidic valve22:18
kanzure-katsmeow: http://heybryan.org/transhumanism_def.html is my rant that will probably reply to most of that link..22:19
kanzure-past all of the greenstuff22:19
* katsmeow fetches that too22:19
kanzure-the entire basis of Hughes' rants and his control of the World Transhumanist Association is that he thinks we're all going to become inequal or something22:26
kanzure-and that people that are uber-rich will leave behind the uber-poor22:26
kanzure-but this is utter nonsense because at the time of his writing it, he was more than well aware of self-replication stuff going on in transhumanist circles22:26
kanzure-although the problems go a little deeper than that22:26
katsmeowwhy is it improving the human body translates to monetary measure?22:27
katsmeownot having cancer doesn't make you rich22:27
katsmeowbeing intelligent doean't make you rich22:27
kanzure-it was basically thinking that "the rich will be able to get their children to have a high IQ"22:27
kanzure-yeah, well, for some reason they correlate IQ and monetary income22:27
kanzure-because of voodoo magic statistics22:27
kanzure-(which I don't really care about of course)22:28
kanzure-so that argument usually goes to "the rich will get richer, the poor will get poorer, waah"22:28
katsmeowwell, until the current bust, that was going on22:29
kanzure-if you're going to assume the domain of transhumanist technologies,22:29
kanzure-then that includes some technologies that make those arguments irrelevant22:29
kanzure-like self-replicators that everyone gets access to22:29
katsmeowthey still trying to make it, some credit cards are saying "either we raise the rates, or you close the card"22:29
kanzure-(not necessarily grey goo nanotech)22:29
kanzure-so what?22:29
katsmeowwell, the poor who don';t have cash will be paying the rich more to borrow that money22:30
kanzure-but why are you assuming money if there are self-replicators running around22:30
katsmeowergo, the rich get more of the money the poor earn22:30
kanzure-I don't see what money has to do with it22:31
katsmeowi was replying to what you said earlier, not to grey goo or replication22:31
katsmeow[22:28] <kanzure-> so that argument usually goes to "the rich will get richer, the poor will get poorer, waah" [22:27] <kanzure-> yeah, well, for some reason they correlate IQ and monetary income22:31
kanzure-it's ok, I still don't understand economics22:32
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katsmeowi don't understand "worth"22:52
fennkanzure there's a flaw in your logic23:05
fennyou're assuming replicators implies everyone will have free access to replicators23:06
genehackerhey kanzure how big of a lense do you really need to do maskless lithography?23:06
genehackerwith an LCD screen23:06
fenngenehacker: approx the size of the LCD23:06
genehackerok23:06
genehackerLCDs can be made quite small23:06
fennso can DMD's...23:07
fennor whatever acronym people are using these days23:07
genehackerplus we can mill our own lenses23:07
genehackerif we really had to23:08
fennyeah, sure23:08
fenni guess you'll be etching your own DMD's too23:08
genehackerfenn, my school had a microchip fab the size of garage23:09
genehackerhttp://www.amazon.co.uk/Bandai-Tamagotchi-Connexion-Black/dp/B0002B9EI623:10
genehackerhow about an LCD this big?23:10
fennthose things have terrible contrast ratio23:11
genehackerbah, we don't need to worry about that23:12
fenngene you should be researching what chemicals are needed for the DNA synthesis and where to get them23:13
genehackeryou don't get them23:13
fennthe LCD/lens stuff is dead simple23:13
genehackeryou make em insitu23:13
fennok, make them, whatever23:13
genehackerone paper said the cost of chemicals was $6023:13
fennyeah, per chip, in quantity23:13
genehackerhard part is the dispensing mechanism23:14
fennyou gotta be kidding23:14
genehackerand you propose to do so how?23:14
fennwell, i'd do it with an inkjet and skip all the lcd stuff23:15
fennbut since you insist on doing it the hard way.. i dunno, a pipette?23:15
genehackeryou need a clean inkjet23:15
fennwhat do they use as substrate? instead of paper or film23:16
fenni mean what keeps the dna from washing away23:16
genehackerhttp://www.biochem.wisc.edu/courses/biochem660/Reading/Singh-Gasson_NatureBiotech_DNAarrays.pdf23:20
genehackersilanized microscope slides23:21
genehackerI don't know fenn23:21
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genehackerI am a bit confused on how the process works23:23
fennso it's in reference 823:24
fenn McGall, G.H. et al. The efficiency of light-directed synthesis of DNA arrays on23:24
fenn glass substrates. J. Am. Chem. Soc. 119, 5081–5090 (1997).23:24
fennjeez23:25
fenn$30.00 for 48 hours of access23:25
fennwhatever happened to buying something outright23:25
genehackerI can see it23:26
genehackerI can't understand it23:26
genehackerhttp://pubs.acs.org/doi/pdf/10.1021/ja964427a?cookieSet=123:27
genehackerkanzure you know what to do23:27
fennnot all of us have institutional access23:27
fennright23:27
genehackerwhat I hate is when I find some really obscure journal23:27
genehackerwith a really cool article23:28
genehackerthat cost $30 to access23:28
fennJ. ACS isn't "really obscure"23:29
genehackerhowever this is:23:33
genehackerhttp://www.actahort.org/members/showpdf?booknrarnr=562_2723:34
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fennyou just want to read it because it says 'fluidic'23:36
fennhell i could build one of those in my sleep :P23:36
genehackerit's an array of fluidic amplifiers hooked up to cheap microphones to detect where bugs are in a silo23:38
fennyeah so?23:38
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any01329918why not use regular amplifiers?23:39
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fennthat would be too easy23:39
katsmeow-afko23:39
genehackerbecause you only have access to crappy microphones23:39
katsmeow-afkso?23:39
genehackerhttp://oai.dtic.mil/oai/oai?verb=getRecord&metadataPrefix=html&identifier=AD072609423:39
genehackerbecause good microphones are expensive23:40
katsmeow-afkfluidic high-intensity sound **generator** concept.23:40
fenni fail to see how a crappy amplifier would make up for a crappy microphone23:42
katsmeow-afkme too23:42
katsmeow-afkthe one presented on that url is 180hz to 2khz23:42
genehackercrappy microphone isn't very sensitibe23:42
katsmeow-afkon the contrary23:42
katsmeow-afkit's not high fidelity, but it canbe very sensitive23:43
genehackerwell the thing is this paper was published in 199723:43
genehackerI am still a bit puzzled as to why they are using fluidic amplifiers too23:44
genehacker1967 I could understand23:44
katsmeow-afkto blast the bejubus outa enemies23:44
katsmeow-afklook at the horn's port size23:45
genehackerhmmm...23:45
genehackerfairly large23:45
katsmeow-afkthe thing could prolly reproduce the space shuttle liftoff at proper volume23:45
katsmeow-afknot all frequencies, tho23:45
genehackerthat's why they make high intensity sound generators23:46
katsmeow-afk[23:43] <genehacker> I am still a bit puzzled as to why they are using fluidic amplifiers too  23:46
katsmeow-afk[23:45] <genehacker> that's why they make high intensity sound generators23:46
katsmeow-afkok, i am confused23:46
katsmeow-afkbbl23:46
genehackerno I am puzzled about the bug detector23:46
genehackerhttp://www.dself.dsl.pipex.com/MUSEUM/COMMS/auxetophone/auxetoph.htm#16523:47

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