| --- Day changed Sun May 10 2009 | ||
| -!- any10968429 is now known as katsmeow | 01:34 | |
| -!- katsmeow is now known as katsmeow-afk | 01:36 | |
| xp_prg | are there any videos yet of this infared protein? | 03:43 |
|---|---|---|
| drazak | fenn: can you name a few assays that use many reagent steps? | 05:11 |
| drazak | you guys know if kanzure is gonna be back any time soon? | 05:18 |
| genehacker | he might | 05:25 |
| drazak | can you guys name a few biochemical assays that use multiple reagent steps in the same container? | 05:26 |
| drazak | ok | 05:35 |
| drazak | so how about this | 05:35 |
| drazak | glass chip | 05:35 |
| drazak | with 2 areas that contain fluid | 05:35 |
| drazak | one of which has a way to add more to it via capilary action, or whatever, the outside one has one of those dna boxes containing two compounds, one that dissolves endothermically, one that disolves exothermically, in the inner fluid area that can have fluids added to it, there are many of those dna boxes present, each with different reagents in it, so that you can do a multitude of tests, depending on what kind of light it's exposed to | 05:37 |
| drazak | the outer fluid area is to provide the proper temperature, such that if you're using something like benedicts reagent you could provide the heat easily | 05:40 |
| drazak | it would be great for quick field tests | 05:41 |
| genehacker | cool | 05:41 |
| genehacker | read some books on microfluidics | 05:41 |
| drazak | I've read up a little on it | 05:42 |
| genehacker | you'll find them very interesting | 05:42 |
| drazak | mhm | 05:42 |
| drazak | I don't have enough time to learn as much as I'd like | 05:42 |
| drazak | maybe this summer | 05:42 |
| * drazak is full time highschool student | 05:42 | |
| drazak | you might be able to make it cheaper by making compound dna boxes | 05:43 |
| drazak | less surfaces | 05:43 |
| drazak | you could also have different boxes full of different reagents in the external area, thus allowing cycles of heating and cooling | 05:45 |
| genehacker | if the boxes are stable enough... | 05:45 |
| drazak | yeah, I thought about that too | 05:46 |
| drazak | I think dna takes a fairly high temperature to denature | 05:46 |
| drazak | and if the boxes form spontaneously, they probably are fairly stable | 05:46 |
| genehacker | how high is high? | 05:47 |
| genehacker | boiling point? | 05:47 |
| drazak | dunno | 05:49 |
| drazak | not enough research done yet | 05:49 |
| genehacker | well I want to make a dna synth over the summer | 05:50 |
| genehacker | a dna synth capable of 62 mega base pairs | 05:50 |
| drazak | you could build these dna boxes with that | 05:50 |
| drazak | I think they're only like a few hundred kbp | 05:50 |
| genehacker | heck I could build J craig venter | 05:51 |
| genehacker | 's synthetic life form with it | 05:51 |
| drazak | haha | 05:51 |
| genehacker | he posted the sequence online | 05:51 |
| drazak | is heybryan offline? | 05:51 |
| genehacker | I need a cheap DLP projector to do it | 05:51 |
| genehacker | I believe he's in meatspace | 05:52 |
| drazak | fuck | 05:52 |
| drazak | I needed to grab the nature paper from him | 05:52 |
| drazak | it'll tell me exactly how many bp it is | 05:52 |
| genehacker | damn | 05:52 |
| genehacker | I don't know if we have it yet | 05:52 |
| drazak | we do | 05:52 |
| drazak | he mentioned it in his email | 05:53 |
| drazak | he even linked it | 05:53 |
| genehacker | damn | 05:53 |
| drazak | yeah | 05:53 |
| drazak | I know | 05:53 |
| drazak | you know, there's another good thing about this | 05:53 |
| drazak | you could make it so that the dissolved reagents are favorablely interacting with the dna box (design the box in such a way) so that they won't go bad (IE oxidize) | 05:54 |
| drazak | need lots of computers to design that crap though | 05:55 |
| genehacker | we have access to a supercomputer down here | 05:56 |
| drazak | mhm | 05:57 |
| genehacker | hmmm... would be synthesizing mycoplasma laboratorium be patent infringement | 05:57 |
| genehacker | write the program and we'll run it | 05:58 |
| drazak | you know what would be cool | 05:58 |
| drazak | you could probably pcr these dna boxes | 05:58 |
| drazak | to make more | 05:58 |
| genehacker | yeah | 05:59 |
| drazak | that would be pretty cool | 06:00 |
| drazak | how do they get dna polymerase A? | 06:02 |
| drazak | (aka the one used in pcr) | 06:03 |
| genehacker | dunno | 06:05 |
| genehacker | that's what we need to make | 06:06 |
| drazak | yeah | 06:06 |
| drazak | that seems to be the expensive part of this whole process, unless I'm missing something | 06:06 |
| drazak | you only need to make each type of box once, or even once in each lab | 06:07 |
| fenn | drazak: taq polymerase is typically synthesized by expression in recombinant e. coli | 07:29 |
| fenn | to build the boxes you'd have to add a digestion step (restriction enzymes) after PCR | 07:30 |
| fenn | if you can get your hands on the taq polymerase sequence, supposedly it's pretty easy to recover the enzyme.. purifying it is another matter though (and i'd try to build some purification into the sequence, like an easily cleave-able binding site) | 07:32 |
| drazak | mhm | 07:43 |
| -!- fenn_ is now known as fenn | 15:47 | |
| -!- any23948583 is now known as katsmeow-afk | 16:17 | |
| -!- any14432598 is now known as katsmeow-afk | 18:06 | |
| -!- any98226738 is now known as katsmeow-afk | 18:12 | |
| -!- any34912125 is now known as katsmeow-afk | 18:18 | |
| -!- any71122580 is now known as katsmeow-afk | 18:39 | |
| fenn | i love this image http://esamultimedia.esa.int/images/spacecraft-operations/space_debris/Bee-Hive-6_H1.jpg | 19:52 |
| drazak | hah | 20:36 |
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