| --- Day changed Tue May 26 2009 | ||
| genehacker | just a funny thing I saw online | 00:00 |
|---|---|---|
| genehacker | convince someone to delete system32 | 00:00 |
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| -!- fenn_ is now known as fenn | 01:25 | |
| fenn | paul really wrote 15 messages to OM today? | 02:09 |
| genehacker | just posted my ez-DNA synth proposal to diybio | 02:12 |
| genehacker | hopefully something useful will come of it | 02:12 |
| fenn | budokai = martial arts society; tenkaichi = best under heaven | 02:47 |
| fenn | literally: heaven below 1 | 02:48 |
| fenn | which would have been easy to figure out if it were written in japanese, btw | 02:49 |
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| faceface | genehacker: good luck! | 04:25 |
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| genehacker | downloadan opera | 09:53 |
| genehacker | installing opera | 09:54 |
| genehacker | does opera every experience periodic flash failure like firefox does? | 09:54 |
| genehacker | done | 09:55 |
| genehacker | I love opera | 10:00 |
| genehacker | well i am now hooked | 10:01 |
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| genehacker | no extensions though | 10:04 |
| kanzure | genehacker: configure it to make tabs on the left-hand side in a readable list instead of at the top | 10:21 |
| kanzure | and then you're all set to go. | 10:21 |
| kanzure | also, with a multi-monitor setup you can devote a monitor to bookmarks or something, since the bookmark manager is kind of spiffy | 10:21 |
| nsh | trust kanz | 10:29 |
| nsh | he knows bookmarking | 10:29 |
| kanzure | Thermodynamic constraints on neural dimensions, firing rates, brain temperature and size | 10:36 |
| kanzure | http://arxiv.org/abs/0905.3690 | 10:36 |
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| kanzure | cis-action: hey | 10:53 |
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| kanzure | how close do you need to get two molecules before a chemical reaction occurs? isn't there some curve for different subatomic forces that are acting on molecules that describes how r is always in the denominator or something? | 11:40 |
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| genehacker | kanzure mechanosynthesis? | 12:25 |
| kanzure | ping | 12:25 |
| kanzure | http://heybryan.org/ | 12:25 |
| kanzure | does this work for anyone | 12:26 |
| genehacker | it loaded 50 milliseconds longer than usual | 12:26 |
| genehacker | it works | 12:26 |
| genehacker | can I get some adblock stuff for opera? | 12:28 |
| genehacker | btw how do browser companies make money? | 12:28 |
| kanzure | opera sells itself to nintendo/wii/cellphones | 12:29 |
| kanzure | there's no adblock for opera :( | 12:29 |
| genehacker | oh | 12:31 |
| genehacker | what about firefox | 12:31 |
| genehacker | ? | 12:31 |
| kanzure | it's free/open source software | 12:31 |
| genehacker | they sell t-shirts I presume? | 12:31 |
| kanzure | Mozilla, the foundation that primarily develops firefox, gets money from Google for putting them as the primary search | 12:32 |
| kanzure | yeah, they sell lots of crap too, but they are largely a non-profit | 12:32 |
| kanzure | so they also work off of donations etc. | 12:32 |
| genehacker | oh | 12:32 |
| genehacker | so that's how | 12:32 |
| genehacker | always wonder | 12:32 |
| genehacker | ed | 12:32 |
| kanzure | well people like me improve firefox for free | 12:32 |
| kanzure | that's what's so great about open source .. people work on it, and then stuff happens | 12:33 |
| genehacker | anyway I need a way to make phosphoramidite nucleosides | 12:33 |
| kanzure | genehacker: you should read more papers from acs.org (american chemical society) about DNA synthesis so that you can get a feel for what the fuck the chemistry is all about | 12:33 |
| genehacker | the papers say how to make the photolabile base things | 12:34 |
| kanzure | there are possibly some simpler reactions for synthesizing DNA that are out there | 12:34 |
| genehacker | here's the deal | 12:34 |
| kanzure | usually the chemical reactions for just about anything are available somewhere via ACS :/ | 12:34 |
| xp_prg | genehacker I am excited by your idea | 12:34 |
| xp_prg | ! | 12:34 |
| genehacker | phosphoramidite nucleosides cost a lot | 12:34 |
| genehacker | do you know why? | 12:34 |
| genehacker | they are really high purity | 12:34 |
| genehacker | did you read my diybio post? | 12:34 |
| kanzure | who are you asking | 12:35 |
| xp_prg | yes I did genehacker | 12:35 |
| genehacker | xp_prg | 12:35 |
| genehacker | so now you understand my evil plan | 12:35 |
| kanzure | ok | 12:35 |
| kanzure | http://heybryan.org/books/papers/ | 12:35 |
| kanzure | it's back up | 12:35 |
| genehacker | so I'd like to know how they get high purity stuff | 12:36 |
| xp_prg | genehacker it sounds way cool! | 12:36 |
| genehacker | from what I hear, they use chromatography | 12:36 |
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| genehacker | it's cool and I believe would could make a DNA synthesizer, we just need to find a way to get the chemicals cheaply so it doesn't cost $100 each time we run it | 12:37 |
| kanzure | genehacker: did you know that your email to diybio was so poorly formatted? | 12:38 |
| genehacker | what? | 12:38 |
| genehacker | how so? | 12:38 |
| kanzure | it had line breaks after every other word | 12:39 |
| kanzure | makes it kind of hard to read | 12:39 |
| genehacker | well I did copy and paste from notepad | 12:39 |
| kanzure | ah, that's why | 12:39 |
| xp_prg | genehacker so you going to make it? | 12:39 |
| kanzure | you need to enable wordwrapping or something | 12:39 |
| kanzure | before you paste | 12:39 |
| kanzure | er, disable wordwrapping I mean | 12:39 |
| genehacker | WHOA | 12:40 |
| genehacker | WTF happened? | 12:40 |
| genehacker | it looked all nice when it posted it in there? | 12:41 |
| kanzure | well it's because you had line breaks | 12:41 |
| kanzure | when gmail sends out email, it splits it up into lines of 72 characters or something | 12:41 |
| kanzure | but if you have a newline character after 72 characters, then the next line will just have "the stuff <new line here> that you want to .." | 12:42 |
| genehacker | ok can I edit my post? | 12:42 |
| kanzure | no | 12:42 |
| kanzure | I suggest resending it | 12:42 |
| genehacker | remove and repost? | 12:42 |
| kanzure | there is no "remove" | 12:42 |
| kanzure | you can try sending it to yourself first if you want to see how it will look | 12:43 |
| genehacker | there is a remove | 12:44 |
| kanzure | where | 12:44 |
| genehacker | on options | 12:46 |
| kanzure | the "report abuse" thing? | 12:46 |
| kanzure | that's for spam. | 12:46 |
| genehacker | no it's not | 12:46 |
| genehacker | reposted | 12:48 |
| genehacker | ugh | 12:48 |
| genehacker | http://groups.google.com/group/diybio/browse_thread/thread/f44fa4989fa799ab | 12:48 |
| genehacker | is that somewhat readable? | 12:49 |
| genehacker | I emailed it to my self and it had spaces.. | 12:49 |
| xp_prg | genehacker you going to make this thing? | 12:51 |
| genehacker | if I can get the chemicals to make it work | 12:51 |
| genehacker | yes | 12:51 |
| kanzure | I need to hop a bus. | 12:52 |
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| ultraleibniz | gah. campbell! | 12:56 |
| * ultraleibniz sets everything up again. | 12:56 | |
| genehacker | is leibniz a computer? | 13:04 |
| ultraleibniz | yes | 13:04 |
| ultraleibniz | http://en.wikipedia.org/wiki/Leibniz | 13:04 |
| genehacker | yeah I know] | 13:04 |
| genehacker | I need to consider working for the ADL... | 13:04 |
| ultraleibniz | it won't work out this summer | 13:04 |
| ultraleibniz | he already hired all of the undergrads that he wants to | 13:04 |
| genehacker | oh that's ok | 13:05 |
| ultraleibniz | however, he was interested in your super microfluidic synthesize-the-shit-out-of-anything design | 13:05 |
| genehacker | writing graph grammars for organic chemistry? | 13:05 |
| ultraleibniz | yeah | 13:05 |
| ultraleibniz | and also probably something involving a component library for the unit processes | 13:05 |
| genehacker | I don't know that much about organic chemistry... | 13:05 |
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| genehacker | heh make the chem e | 13:06 |
| genehacker | s obsolete | 13:06 |
| kanzure | did you see the retrosynthetic route designer paper thingy? | 13:06 |
| ultraleibniz | http://heybryan.org/books/papers/Route%20designer%20-%20a%20retrosynthetic%20analysis%20tool%20utilizing%20automated%20retrosynthetic%20rule%20generation.pdf | 13:06 |
| kanzure | drazak_: can you find me any references on physical imprints of the size/shape of different nucleotides? | 13:07 |
| kardan| | hi, whats a AFM cantilever? | 13:10 |
| kardan| | (just read some pages back) | 13:11 |
| kanzure | do you know what AFM is? | 13:11 |
| kanzure | AFM is atomic force microscopy- one of the forms of analytical instrumentation- | 13:11 |
| kanzure | http://heybryan.org/instrumentation/instru.html | 13:12 |
| kanzure | where you use a super-fine tipped needle to poke a surface | 13:12 |
| kanzure | using a very flimsy reflective 'cantilever', or 'long rod looking thingy' you can detect how much the reflective film bends due to force on the super-fine tipped needle | 13:13 |
| kanzure | you can detect this by using a laser shinning at the cantilever. based off of the angle of deflection, you can figure out how much force is being exerted on your tip. | 13:13 |
| kanzure | you detect the angle of detection via (typically) a four-segmented photodiode | 13:13 |
| kanzure | although there are some that use CMOS image sensors or whatever | 13:13 |
| kanzure | er, angle of deflection (sorry) | 13:15 |
| kardan| | thats what i am interested too | 13:15 |
| kanzure | are you the one that doesn't speak english? | 13:15 |
| kanzure | :p | 13:16 |
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| bluekanzure | "username 'kanzure' is already registered on arxiv.org" | 13:48 |
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| kanzure | On Tue, May 26, 2009 at 3:00 PM, BillK <pharos@gmail.com> wrote: | 15:20 |
| kanzure | > If you don't have all the legally required animal testing licenses, | 15:20 |
| kanzure | > premises inspections, animal care permits, etc. then what you are | 15:20 |
| kanzure | > doing is torturing animals for a hobby. | 15:20 |
| Utopiah | watch out, in few days you might have hot celebrities from PETA pounding on your door... | 15:25 |
| kanzure | do I get to bone them? | 15:26 |
| Utopiah | only if you don't label that action "animal experiment" | 15:28 |
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| Utopiah | (starting to sound a bit awkward) | 15:28 |
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| kanzure | > But what value do you think such research will have? | 15:57 |
| kanzure | > 'Here, take these pills. A friend of mine fed them to some rabbits in | 15:57 |
| kanzure | > Indonesia and they seemed to be stronger than usual'. Really??? | 15:57 |
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| genehacker | WOOHOO | 16:52 |
| genehacker | I have a damn tiny water pump! | 16:53 |
| kanzure | a tiny wong? | 16:55 |
| genehacker | no | 16:56 |
| genehacker | I'm making a shirt with a heat exchanger | 16:57 |
| genehacker | I needed a pump that would be small yet powerful enough to drive coolant through ~15 feet of aquarium tubing so I don't have to be hot in the summer | 16:58 |
| genehacker | So I found a $5 motorized water gun | 16:58 |
| fenn_ | where did you find that? | 16:59 |
| -!- fenn_ is now known as fenn | 16:59 | |
| genehacker | happens to have a fitting that fits the aquarium tubing, on one end... | 16:59 |
| genehacker | Toys R Us | 16:59 |
| genehacker | went all over town looking for something like that | 17:00 |
| genehacker | hmmm... | 17:00 |
| genehacker | wonder if that pumps strong enough to drive my flamethrower... | 17:00 |
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| fenn | is it just me or are squirt guns steadily progressing towards 'covenant plasma rifle'? http://rsk.imageg.net/graphics/product_images/pRS1C-2305387w345.jpg | 17:02 |
| genehacker | it is but just barely | 17:10 |
| genehacker | it is strong enough but my flamethrower is a bit leakyier than anticipated | 17:10 |
| genehacker | it is not just you | 17:11 |
| genehacker | http://www.amazon.com/gp/product/B000LZFTY6/ref=s9_simx_gw_s0_p21_t2?pf_rd_m=ATVPDKIKX0DER&pf_rd_s=center-2&pf_rd_r=00K0WBGFZHZ3EJ70VN0R&pf_rd_t=101&pf_rd_p=470938631&pf_rd_i=507846 | 17:13 |
| genehacker | http://halo3blogs.files.wordpress.com/2009/03/plasmarifle-halo.jpg | 17:13 |
| fenn | the wikipedia article on water guns is pretty awesome | 17:13 |
| genehacker | I'm document this stuff | 17:14 |
| genehacker | I'm going to document this pump | 17:14 |
| genehacker | a water gun that uses a peristaltic pump? | 17:15 |
| genehacker | interesting | 17:15 |
| fenn | it's only to be expected | 17:16 |
| fenn | white tubing? about 1/8"? | 17:16 |
| genehacker | 0.17 inch | 17:16 |
| genehacker | also this watergun was not meant to be dissassembled | 17:17 |
| genehacker | part of the plastic was ultrasonically welded | 17:17 |
| fenn | try using a brazilian screwdriver | 17:18 |
| genehacker | a crow bar? | 17:25 |
| genehacker | I just use my bear hands | 17:25 |
| fenn | i didnt know you were a raccoon | 17:27 |
| fenn | this is much harder to make it work than it seems: http://en.wikipedia.org/wiki/Assassin_(game) | 17:29 |
| genehacker | article = dead | 17:30 |
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| genehacker_ | you're already registered in arxiv?' | 17:36 |
| fenn | genehacker_: what article is dead? | 17:36 |
| genehacker_ | the assasin game | 17:36 |
| fenn | copy and paste the link | 17:37 |
| genehacker_ | what is a brazilian screwdriver? | 17:38 |
| genehacker_ | a knife | 17:38 |
| fenn | a hammer | 17:40 |
| genehacker_ | oh | 17:40 |
| fenn | ... i think. i doubt anybody really knows | 17:40 |
| fenn | how come texas doesnt have anything like this? http://en.wikipedia.org/wiki/Songkran | 17:46 |
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| genehacker_ | we do | 17:53 |
| genehacker_ | acouple months ago with water balloons and colored powder instead of chalk | 17:53 |
| genehacker_ | that was the indian students association thing though | 17:54 |
| genehacker_ | so I don't know really | 17:54 |
| fenn | it's not hot here in april though | 17:54 |
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| genehacker_ | oops | 17:54 |
| genehacker_ | what did I do? | 17:54 |
| fenn | you used a web browser | 17:55 |
| genehacker_ | no | 17:55 |
| genehacker_ | I hit one of the exit keys | 17:55 |
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| fenn | genehacker_: do you know how to use ssh? | 17:57 |
| genehacker_ | no | 17:57 |
| genehacker_ | tell me | 17:58 |
| genehacker_ | hmmm | 17:58 |
| genehacker_ | pump is an impellor type | 17:58 |
| fenn | er, well on a windows machine you'd download putty.exe | 17:58 |
| genehacker_ | ok | 17:58 |
| fenn | nevermind, i'm just wondering why my mail server is rejecting ssh connections | 17:59 |
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| drazak_ | kanzure: what do you want me to look up? | 18:18 |
| kanzure | drazak_: how to make a hole in which only a certain nucleotide can become stuck in | 18:21 |
| drazak_ | could you use part of the substrate section from a reverse transcriptase? | 18:22 |
| kanzure | drazak_: just so I get things straight, your name is ben too? | 18:22 |
| drazak_ | yeah | 18:22 |
| kanzure | why is this channel so heavily b-biased when it comes to letters of first names | 18:23 |
| drazak_ | b for brain | 18:23 |
| kanzure | drazak_: possibly, if you could find one of those papers that studies the angstrom-scale structure via x-ray crystallography or something, it might be a start | 18:23 |
| drazak_ | yeah | 18:23 |
| drazak_ | stupid java application | 18:23 |
| drazak_ | won't let me alt tab ut of it | 18:24 |
| kanzure | "help! I'm trapped in a java applet!" condolences. | 18:24 |
| kanzure | yay for Austin Che | 18:24 |
| kanzure | http://www-lecb.ncifcrf.gov/~toms/latex.html | 18:24 |
| drazak_ | wtf | 18:25 |
| drazak_ | isn't makedir the command to make a folder? | 18:25 |
| * drazak_ losing it | 18:25 | |
| kanzure | mkdir | 18:25 |
| drazak_ | thanks | 18:25 |
| kanzure | now you must sacrifice the gods | 18:25 |
| drazak_ | I'm just trying to get music onto my fucking mp3 player | 18:25 |
| drazak_ | it's a bitch | 18:25 |
| kanzure | *to the gods | 18:25 |
| drazak_ | it won't transfer by usb because it's half broken, so I have to plug the base into the network | 18:26 |
| fenn | sounds like it needs an exorcism | 18:26 |
| kanzure | apple be gone? | 18:27 |
| fenn | the worm that ate the apple | 18:27 |
| drazak_ | it's a rio karma | 18:27 |
| fenn | ah, well if it's karma then there's nothing we can do, since obviously you deserve it | 18:28 |
| kanzure | fenn: re: lisp stuff, I've continued to just comment the living hell out of the file and it seems to be making me think I'm making progress | 18:28 |
| kanzure | whether or not there actually is any progress only time will tell | 18:28 |
| kanzure | er, debug-print-statement-style comments | 18:28 |
| fenn | well, here's how you know if you have someting useful | 18:28 |
| kanzure | if it works? | 18:29 |
| fenn | if you can feed it data and it spits out te result of some calculation | 18:29 |
| kanzure | right. | 18:29 |
| fenn | for example, f(x) = x^2 | 18:29 |
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| fenn | hullo there splicer | 18:30 |
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| genehacker_ | now where do I get 5/8 in diameter pipe? | 18:30 |
| genehacker_ | od of course | 18:30 |
| splicer | hi fenn | 18:30 |
| kanzure | the fact that you have to ask "where do I get this standard-sized part" tells you that something is wrong with the system | 18:31 |
| fenn | ey i had this idea too (probably aroun te same time, must be te hylaean theoric wave front) http://www.ccrnp.ncifcrf.gov/~toms/patent/molecularrotationengine/ | 18:31 |
| genehacker_ | yeah | 18:31 |
| kanzure | is that the US obfuscation organization agency? | 18:31 |
| drazak_ | finally | 18:31 |
| drazak_ | got it transfering | 18:31 |
| drazak_ | but I think the base is only 10mbps | 18:32 |
| fenn | kanzure: no it's a theory of why everyone comes up with te same tings at the same time | 18:32 |
| genehacker_ | is that a brownian rectifier? | 18:32 |
| kanzure | fenn: no, the TLD/domain name in the URL. | 18:32 |
| kanzure | ccrnp ncifcrf gov | 18:32 |
| fenn | it's a muscle fiber powered rotary engine | 18:32 |
| genehacker_ | WHOA | 18:32 |
| kanzure | ah neat | 18:33 |
| kanzure | I'm glad somebody has done this | 18:33 |
| genehacker_ | I wonder if this is more efficient than muscle | 18:33 |
| kanzure | how much power do you get out | 18:33 |
| genehacker_ | I wonder if one was ever built | 18:33 |
| drazak_ | any of you dudes going to be in boston over the summer? | 18:33 |
| genehacker_ | muscle is about 25% efficient | 18:33 |
| fenn | drazak_: i wish | 18:33 |
| kanzure | drazak_: I will be there in spirit | 18:34 |
| drazak_ | I might be a research assistant in boston | 18:34 |
| drazak_ | for my uncle | 18:34 |
| fenn | where does your uncle work? | 18:35 |
| drazak_ | uh | 18:35 |
| drazak_ | research at harvard | 18:35 |
| kanzure | isn't that cheating | 18:35 |
| drazak_ | a little :P | 18:36 |
| drazak_ | narcissism is horrible until it happens to you! | 18:36 |
| fenn | nepotism | 18:36 |
| drazak_ | whatever | 18:36 |
| splicer | hehe | 18:36 |
| fenn | nihilism | 18:36 |
| genehacker_ | hmmm... | 18:36 |
| genehacker_ | I wonder if this pump would be submersible | 18:37 |
| * drazak_ nihilist | 18:37 | |
| drazak_ | kanzure: what was the enzyme called? | 18:38 |
| genehacker_ | GASP-1? | 18:38 |
| kanzure | for what | 18:38 |
| drazak_ | kanzure: the one ofr cleaving da 1 nucleotide at a time | 18:38 |
| kanzure | it's related to an endonuclease | 18:38 |
| kanzure | not a ligase .. it's a... er.. | 18:38 |
| kanzure | line | 18:39 |
| drazak_ | fenn: were you there when I talked to him last night? | 18:39 |
| fenn | hether for art thou, romeo | 18:39 |
| fenn | drazak_: yes | 18:40 |
| drazak_ | what enzyme did he say? | 18:40 |
| fenn | i wasn't really listening | 18:40 |
| kanzure | have I been making up enzymes again? | 18:40 |
| drazak_ | maybe | 18:40 |
| kanzure | I could have sworn this exists | 18:40 |
| kanzure | you could use an endonuclease to cleave off one nucleotide if you attach the cleavage site to it | 18:40 |
| drazak_ | ok | 18:41 |
| drazak_ | I'll just say that | 18:41 |
| drazak_ | I'm trying to further impress my uncle so that he'll take me | 18:41 |
| drazak_ | :P | 18:41 |
| fenn | "A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids." | 18:41 |
| kanzure | drazak_: the real way to impress him is to read all of his papers and cite it in your proposal or something | 18:42 |
| kanzure | what is his name / link? | 18:42 |
| fenn | Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, in contrast to exonucleases, which cleave phosphodiester bonds at the end of a polynucleotide chain. | 18:42 |
| drazak_ | kanzure: e finkelstein | 18:42 |
| kanzure | seriously? | 18:43 |
| drazak_ | yes? | 18:43 |
| kanzure | hehe | 18:43 |
| drazak_ | you know his papers? | 18:43 |
| drazak_ | he works on lab on a chip stuff | 18:43 |
| kanzure | hm. | 18:44 |
| kanzure | no. | 18:44 |
| drazak_ | he married into the family | 18:44 |
| fenn | i dont see any E. Finkelsteins | 18:44 |
| drazak_ | I'm not a finkelstein | 18:44 |
| kanzure | the results on google scholar are nil | 18:44 |
| kanzure | does this guy exist? | 18:44 |
| drazak_ | hold on | 18:45 |
| splicer | https://www.researchgate.net/author/E+Finkelstein | 18:45 |
| drazak_ | he goes by eb finkelstein | 18:45 |
| drazak_ | I thought he did e finkelstein | 18:45 |
| kanzure | I only see: The role of VEGF isoforms in tumor angiogenesis | 18:45 |
| drazak_ | yeah | 18:46 |
| drazak_ | I saw that one too | 18:46 |
| drazak_ | when he was working in syracuse | 18:46 |
| drazak_ | his stuff is like darpa now | 18:46 |
| drazak_ | so you might have to search patents | 18:46 |
| kanzure | urhm. | 18:46 |
| drazak_ | http://www3.interscience.wiley.com/journal/104084493/abstract | 18:46 |
| fenn | yay SCIENCE | 18:46 |
| kanzure | fenn: do you have a UT login yet? | 18:47 |
| drazak_ | http://www.informaworld.com/smpp/content~content=a713811278~db=all | 18:47 |
| kanzure | http://ezproxy.lib.utexas.edu/login?url=guess-what | 18:47 |
| fenn | kanzure: sort of; they wont let me use it for anything | 18:47 |
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| kanzure | fenn: surely they will let you use the library's online services? | 18:47 |
| fenn | oh, wow | 18:48 |
| fenn | ok how do i use this | 18:49 |
| kanzure | did it log you in? | 18:49 |
| kanzure | it's just a proxy, so it does a search-and-replace on all URLs or something | 18:49 |
| kanzure | and lets you access PDFs from the super-evil journals | 18:50 |
| drazak_ | I know my friends university access | 18:50 |
| drazak_ | I can use it to search | 18:50 |
| kanzure | I wish I would have had a friend back in the day who gave me university access to scholarly literature | 18:51 |
| kanzure | back in high school I had to steal it from the taliban or something | 18:51 |
| fenn | i got a pdf! | 18:51 |
| drazak_ | rofl | 18:51 |
| kanzure | go pokeball! | 18:51 |
| drazak_ | I lost it though | 18:51 |
| drazak_ | :( | 18:51 |
| fenn | good old taliban password bbs'es | 18:52 |
| fenn | we would trade opium and RPG's for journal articles | 18:52 |
| drazak_ | haha | 18:52 |
| splicer | maybe they are | 18:53 |
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| splicer | is it true that lab assistants are underpaid? | 18:55 |
| kanzure | yes | 18:55 |
| fenn | how could it not be true? | 18:56 |
| drazak_ | I'll probably work for free | 18:57 |
| drazak_ | I'd be staying with him and my aunt in boston | 18:57 |
| splicer | somehow i thougt it was a bit like the software business... not that most people make a lot of money.. but they get by at least. | 18:57 |
| fenn | drazak_: make sure to go spy on the media lab for us | 18:57 |
| genehacker_ | TELL US EVERYTHING | 18:57 |
| drazak_ | I'm going to spy on the whole thing | 18:57 |
| drazak_ | they're growin HEARTS | 18:57 |
| fenn | and MINDS | 18:57 |
| drazak_ | and BONES | 18:57 |
| drazak_ | and KIDNEYS | 18:57 |
| genehacker_ | I could use a second heart | 18:58 |
| genehacker_ | that way I could be just like doctor who | 18:58 |
| drazak_ | lol | 18:58 |
| genehacker_ | always build in redundancy... | 18:58 |
| fenn | sigh.. all my old muds are wiped from the face of the internet | 18:59 |
| splicer | where would you put it? | 18:59 |
| drazak_ | and he's growing skeletal muscle | 18:59 |
| fenn | genehacker_: and make it run on a fusion reactor core so you dont have to recharge it every twenty minutes | 19:00 |
| kanzure | is it wrong that I've played more MOOs than MUDs? :( | 19:00 |
| genehacker_ | now how I'm I supposed to build a handheld switch and battery case that doesn't look like a trigger to a suicide bomber vest? | 19:00 |
| kanzure | just give it curves | 19:00 |
| fenn | it's mostly a matter of which one you were exposed to first, i think | 19:00 |
| genehacker_ | deabbriviate those acronyms plz | 19:01 |
| kanzure | fenn: unfortunately, I was exposed to neither | 19:01 |
| fenn | either one will get you humiliated in public though, so i dont really see the difference | 19:01 |
| kanzure | but for some reason I said hello on the Artemis Society MOO server | 19:01 |
| fenn | MOO is more interactive | 19:01 |
| kanzure | ah, that's right, MUDs had more backend servery goodness | 19:01 |
| fenn | MUD is more .. solid | 19:01 |
| genehacker_ | That's it | 19:02 |
| fenn | sort of like secondlife vs WoW | 19:02 |
| genehacker_ | I'll use a reed switch | 19:02 |
| genehacker_ | oh | 19:02 |
| genehacker_ | deabriviation plz? | 19:02 |
| fenn | multi user dungeon/dimension, MUD object oriented (i guess) | 19:02 |
| fenn | it doesnt really elp does it | 19:02 |
| fenn | an IRC channel is like one 'room' or grid coordinate in a virtual mud world | 19:03 |
| fenn | but on IRC we can only talk and emote (/me) | 19:03 |
| fenn | whereas in a mud there are also items and actions and state variables like hitpoints etc | 19:04 |
| kanzure | er, wait, did MUDs do IRC integration? | 19:04 |
| drazak_ | lol | 19:04 |
| kanzure | it was like interactive fiction on steroids | 19:04 |
| fenn | i wouldnt be surprised, but usually not | 19:04 |
| kanzure | IIRC | 19:04 |
| drazak_ | this is takin for fucking ever | 19:04 |
| fenn | there was a mud-wide IRC channel, channel for each clan or guild or whatever | 19:04 |
| fenn | er, except it wasnt IRC | 19:04 |
| fenn | the protocol i mean | 19:04 |
| fenn | i think my mind was permanently shaped by early exposure to muds | 19:05 |
| fenn | and that's why i feel comfortable with a command line (it's how i learned to type after all) | 19:05 |
| splicer | people used to use muds as irc:s | 19:06 |
| fenn | VieMUD was heavily influenced by unix concepts | 19:06 |
| fenn | you could do variable substitution, set up aliases, shell history search, etc | 19:06 |
| fenn | and of course tab completion | 19:06 |
| kanzure | huh | 19:07 |
| kanzure | sounds nice. | 19:07 |
| kanzure | sadly I learned to roleplay on forums .. not quite the best place. | 19:07 |
| fenn | my condolences | 19:07 |
| kanzure | must increase post count .. | 19:07 |
| kanzure | to get to level 2.. | 19:07 |
| kanzure | blah. | 19:07 |
| genehacker_ | I don't roleplay | 19:07 |
| fenn | anyway viemud was written in plain C and suffered under the decades of code hacking | 19:08 |
| fenn | eventually it was down to just one Implementor | 19:08 |
| kanzure | much like nethack? | 19:08 |
| kanzure | heh | 19:08 |
| fenn | nethack is gross | 19:08 |
| fenn | more like interactive storytelling | 19:08 |
| kanzure | fenn: I introduced you to Bill at The Castilian. he just sent me a link over to this feynman/connection-machine article: http://www.longnow.org/views/essays/articles/ArtFeynman.php | 19:08 |
| fenn | but with guns and swords | 19:08 |
| kanzure | nice to see someone I've known since high school reading longnow.org | 19:08 |
| genehacker_ | I like nethack | 19:09 |
| kanzure | you play nethack? | 19:09 |
| genehacker_ | I played it a little | 19:09 |
| genehacker_ | I have it on my DS | 19:09 |
| kanzure | wtf | 19:09 |
| genehacker_ | but it doesn't work with my flashcart | 19:09 |
| genehacker_ | ever hear of homebrew? | 19:09 |
| kanzure | yeah | 19:10 |
| kanzure | of course .. we've all done our own gameboy color games at one point or another | 19:10 |
| genehacker_ | I haven't yet... | 19:10 |
| kanzure | hex hacking nastolgia. | 19:10 |
| fenn | it's really kind of amazing how many awesome ideas people have ad in the past that were just too early | 19:10 |
| genehacker_ | too much freestuff | 19:10 |
| fenn | stuff like transputer/connection machine, or engelbart's stuff | 19:10 |
| genehacker_ | too little freetime | 19:10 |
| genehacker_ | who is this bill person? | 19:11 |
| kanzure | high school friend | 19:11 |
| drazak_ | jesus fuck | 19:12 |
| kanzure | he's smart, but not in the way that makes him do anything | 19:12 |
| drazak_ | this album is a fucking gig | 19:12 |
| kanzure | but he doesn't do nothing in the same sense that I do nothing | 19:12 |
| kanzure | he seems to just play guitar or something :p | 19:12 |
| genehacker_ | well he might be useful | 19:13 |
| genehacker_ | one of my friends who builds ridiculous custom nerf guns also happens to play guitar | 19:13 |
| fenn | heh "Instead, we planned to connect the processors in a 20-dimensional hypercube" | 19:13 |
| kanzure | if you want to talk with him, he's bluesplayer@gmail.com | 19:13 |
| genehacker_ | he's trying to make his own dart factory | 19:13 |
| kanzure | OR! a 20-d TIMECUBE! yeah! | 19:14 |
| genehacker_ | haha time cube | 19:14 |
| genehacker_ | The time cube ⬖ ⬗ ⬘ ⬙ | 19:14 |
| genehacker_ | THE GOVERNMENT ENCODED IT INTO UNICODE | 19:14 |
| kanzure | isn't unicode ISO, and not US govt? | 19:15 |
| fenn | i, er, yeah | 19:15 |
| splicer | kanzure: Have you directed a swedish transhumanist named Alexander my way? | 19:15 |
| kanzure | splicer: no, I don't think so | 19:15 |
| splicer | k.. thanks | 19:15 |
| kanzure | splicer: btw, do you know Anders Sandberg yet? | 19:15 |
| fenn | there was someone in here trying to start wta.se? or transhumanism.se maybe | 19:15 |
| kanzure | http://aleph.se/ | 19:15 |
| splicer | yeah... alexander wanted transhumanism.se | 19:15 |
| fenn | or maybe e was trying to make transhumanism.se more general and less biohacking focused | 19:15 |
| splicer | so sandberg was here? | 19:16 |
| kanzure | it's sad that Anders is just an ethicist at Oxford now | 19:16 |
| kanzure | no, sandberg never talks with me | 19:16 |
| splicer | i don't know any of them | 19:16 |
| fenn | them? | 19:19 |
| splicer | kanzure is the only one who sometimes calls himself transhumanist i know | 19:19 |
| kanzure | I'd rather be a transhuman than an -ist :( | 19:20 |
| splicer | i think their time has passed... but thats another topic | 19:20 |
| splicer | i think it was probably great to be an extropian in the 80:ies | 19:21 |
| fenn | pfff | 19:21 |
| kanzure | what did they accomplish? | 19:21 |
| fenn | the 2080's maybe | 19:21 |
| fenn | if we're lucky | 19:22 |
| splicer | they were a think tank... they had a lot of fun | 19:22 |
| fenn | kanzure: they wrote a bunch of books | 19:22 |
| fenn | and had some newsgroup flame wars | 19:22 |
| fenn | about the same as now, pretty much | 19:22 |
| splicer | it was the time to have flamewars | 19:23 |
| kanzure | I should start using history as an example | 19:23 |
| kanzure | "how is this any different from the 80s" | 19:23 |
| fenn | it just seems so much more grand because we get a whole decade compressed into a single wiki article | 19:23 |
| splicer | the garage biology culture needs to get started... we need the new apples and hp:s | 19:26 |
| kanzure | how can we make sure that doesn't happen to me | 19:26 |
| kanzure | splicer: nobody cares enough to listen to us | 19:26 |
| kanzure | everybody's too busy talking about the ethics of biohacking and other bullshit | 19:26 |
| kanzure | rather than actually fixing any of the problems they are talking about | 19:26 |
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| splicer | yup... it's like everyone thinks there are scientists in white lab coats taking care of business. | 19:27 |
| genehacker_ | well that's what I'm trying to do with my DNA synth project | 19:27 |
| genehacker_ | no response in diybio | 19:28 |
| kanzure | nobody has replied to you | 19:28 |
| genehacker_ | because of the horrible formatting? | 19:28 |
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| kanzure | the second time you sent it, it looked better | 19:29 |
| kanzure | so now it's just because people are lazy fucks | 19:29 |
| bkero | kanzure: yo | 19:29 |
| genehacker_ | still it's a big pile of words | 19:29 |
| genehacker_ | maybe if I make it easier to read | 19:29 |
| genehacker_ | I'll get a response | 19:30 |
| fenn | genehacker_: i'm pretty certain you'll have to build a working model before anyone gets excited | 19:30 |
| genehacker_ | I need some damn phosphoramidite nucleosides! | 19:30 |
| genehacker_ | ok | 19:30 |
| fenn | so steal some | 19:30 |
| genehacker_ | hmmm | 19:31 |
| genehacker_ | so first I need to find out if they have a DNA synth here on campus, then I have to find a way to disable the alarm system... | 19:32 |
| kanzure | ellington has one | 19:32 |
| genehacker_ | he does | 19:32 |
| genehacker_ | hmmm... | 19:32 |
| kanzure | do you want the operating manuals? | 19:32 |
| genehacker_ | nah I can find those online | 19:32 |
| kanzure | how could you if you don't know the model? | 19:32 |
| genehacker_ | but if you have em in PDF form send them to me | 19:32 |
| fenn | i suspect the nucleosides will be in bottles in a cabinet next to the macine | 19:32 |
| kanzure | or in the refirdgerator | 19:33 |
| genehacker_ | because I already found one | 19:33 |
| splicer | they are expensive i think | 19:33 |
| kanzure | refrigerator | 19:33 |
| kanzure | you found one what? | 19:33 |
| genehacker_ | they will be in four different bottles connected to the machine | 19:33 |
| kanzure | not if they aren't loaded. | 19:33 |
| kanzure | also, it's more than four bottles | 19:33 |
| kanzure | those are the dNTPs | 19:33 |
| genehacker_ | they tend to stay loaded | 19:33 |
| genehacker_ | what is that? | 19:34 |
| kanzure | nucleotides. | 19:34 |
| genehacker_ | perhaps we could borrow some | 19:34 |
| genehacker_ | legitly borrow some | 19:34 |
| fenn | sort of like borrowing a cup of sugar | 19:34 |
| kanzure | http://heybryan.org/books/ellingtonlab/A_E-Lab_Stuff/Software/Programs/ | 19:35 |
| fenn | you have to say it like 'pardon me, do you have any grey poupon' | 19:35 |
| genehacker_ | if we could make a microfluidic instant DNA synth we could ask for about 5 ml and we might be set for life | 19:36 |
| kanzure | eh | 19:36 |
| kanzure | you apparently don't know about debugging yet | 19:36 |
| genehacker_ | yeah... | 19:37 |
| genehacker_ | I wonder how much stuff is used up each nucleotide cycle with the maskless litho thing? | 19:37 |
| fenn | depends how much you add | 19:39 |
| fenn | higher concentration = faster reaction kinetics | 19:39 |
| kanzure | why not vary temperature, pressure, concentration, etc. | 19:39 |
| kanzure | I don't know why I don't see more experiments done under high pressure or something | 19:40 |
| kanzure | wouldn't it be advantageous? | 19:40 |
| fenn | temperature might cause bad side reactions like depolymerization | 19:40 |
| fenn | pressure is expensive and somewhat dangerous | 19:40 |
| genehacker_ | huh | 19:40 |
| fenn | you have to get into GPa to really see weird cemical reactions | 19:41 |
| kanzure | not in a microfluidic system. you could apply pressure by turning a screw, or putting a giant brick on it or something | 19:41 |
| fenn | unless they're gas phase reactions | 19:41 |
| genehacker_ | that is interesting because take a look at this: | 19:41 |
| kanzure | fenn: GPa is probably easier to reach in smaller volumes | 19:41 |
| fenn | of course | 19:42 |
| drazak_ | deacronym | 19:42 |
| fenn | giga pascal = 145000 psi | 19:42 |
| drazak_ | oh | 19:42 |
| drazak_ | psi no help, but ok | 19:42 |
| fenn | pounds per square inc | 19:42 |
| genehacker_ | hold on | 19:42 |
| kanzure | I've sometimes thought about a method to make GPa or kPa at least with your finger force | 19:42 |
| kanzure | but in sufficiently small volumes or something | 19:42 |
| drazak_ | I know what psi IS | 19:42 |
| drazak_ | oh right | 19:43 |
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| genehacker_ | point is in the dna chips by maskless lithography patent, the reaction chamber looks like it is built for high pressure | 19:43 |
| drazak_ | 101.7kpa=atm gigapascal=10000atm | 19:43 |
| genehacker_ | or that it was over engineered | 19:43 |
| fenn | what makes you say that | 19:44 |
| genehacker_ | well when I find it you'll see | 19:45 |
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| fenn_ | gah | 19:46 |
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| fenn | so you were making empty threats about your dna synthesizer | 19:47 |
| splicer | there is probably a very elegant dna synthetization method just waiting to be discovered | 19:47 |
| splicer | somewhere | 19:47 |
| fenn | i have it | 19:47 |
| splicer | the light thing? | 19:47 |
| fenn | yes | 19:48 |
| fenn | could it get any more elegant? | 19:48 |
| splicer | you may be right | 19:48 |
| genehacker_ | blast | 19:48 |
| fenn | if a mechanical computer were integrated somehow, peraps (not more elegant but infinitely more useful) | 19:48 |
| genehacker_ | I'm having trouble finding it | 19:48 |
| genehacker_ | mechanical integrators? | 19:49 |
| genehacker_ | they exist | 19:49 |
| genehacker_ | mechanical differential integrators | 19:49 |
| fenn | mechanical computer + laser diodes + dna read/write head | 19:49 |
| fenn | then you have a network between cells, eac cell is a network node | 19:49 |
| genehacker_ | maskless photolithography gene synthesis is pretty elegant | 19:50 |
| fenn | laser link being te physical layer, sort of like wifi since you're below the diffraction limit | 19:50 |
| fenn | then the computer could demultiplex single-frequency light, or other data transmission schemes | 19:50 |
| fenn | instead of the write head being "hard wired" to write an A on 440 nm or whatever | 19:51 |
| genehacker_ | http://www.google.com/patents?id=YYIKAAAAEBAJ&dq=method+and+apparatus+for+synthesis+of+arrays+of+dna+probes | 19:52 |
| fenn | there is someting hard stuck underneath my "h" key and i dont know how to get it out without breaking the key off | 19:52 |
| genehacker_ | take a look at this | 19:52 |
| kanzure | fenn: laptop keyboards can be removed | 19:52 |
| genehacker_ | mechanical computers need to be made very accurately in order to be accurate | 19:52 |
| fenn | yeas.. rmm but the laptop would probably disintegrate and i'll never get it back together | 19:52 |
| splicer | fenn: the print head is a protein? | 19:52 |
| fenn | splicer: yes, protein complex | 19:52 |
| genehacker_ | are laptop keyboards not supposed to be removable? | 19:53 |
| kanzure | no, they are generally replaceable | 19:53 |
| fenn | splicer: like PolIII but with some antennae stuck on it (in the hard wired version) | 19:53 |
| genehacker_ | Dell laptop keyboards are? | 19:53 |
| kanzure | maybe. you should check the screws. | 19:53 |
| splicer | fenn: checking | 19:53 |
| genehacker_ | see sheet seven on the patent | 19:54 |
| genehacker_ | that slide is bolted down | 19:54 |
| genehacker_ | oh | 19:54 |
| genehacker_ | for alignment | 19:54 |
| fenn | genehacker_: what is the patent number? | 19:55 |
| fenn | oh nm i see google now allows you to download a pdf | 19:56 |
| fenn | splicer: the green and purple blob in http://www.youtube.com/watch?v=Y6FitehwJg4 | 19:58 |
| genehacker_ | yeah | 19:58 |
| genehacker_ | which I why I like google patent | 19:58 |
| fenn | splicer: you'd have to supply it with a fake template strand | 19:58 |
| fenn | or else modify the enzyme someow so that the template strand won't interfere with bases in the new strand | 19:59 |
| kanzure | conformational changes to make it only allow certain template nucleotides would be a nice way to go about it | 19:59 |
| kanzure | then you immobilize the polymerase to an electrode or something | 19:59 |
| fenn | and move the dna around? that would take forever | 20:00 |
| kanzure | polymerase moves the dna | 20:00 |
| splicer | fenn: thanks | 20:00 |
| fenn | exponentially forever | 20:00 |
| kanzure | er | 20:00 |
| kanzure | polymerase moves it I thought? | 20:00 |
| kanzure | is there any immobilized polymerase function studies out there? | 20:00 |
| kanzure | I don't think I've seen any | 20:00 |
| fenn | polymerase moves along the template strand like a train on train tracks | 20:00 |
| fenn | yes, i'm sure there are | 20:01 |
| kanzure | ok, so you don't have to move the strand then | 20:01 |
| kanzure | the train doesn't move the planet :p | 20:01 |
| kanzure | unless you're watching futurama | 20:01 |
| fenn | the pacific biosciences<?> sequencer works with an immobilized polymerase | 20:01 |
| fenn | kanzure: generally DNA is much higher molecular weight than the polymerase molecule | 20:01 |
| kanzure | bah, it's a strong 'lil fighter | 20:02 |
| fenn | so i say the polymerase moves along the DNA | 20:02 |
| fenn | now, in some PCR reaction it might be different | 20:02 |
| fenn | but really who cares | 20:02 |
| kanzure | I think an electrical method to change the conformation of an immobilized polymerase is somewhat more likely to work than engineering a laser/wavelength conformational system | 20:03 |
| kanzure | if only because you can immobilize the polymerase and already have it at a specific location or something | 20:03 |
| fenn | you still have to figure out how to make the correct conformational change, which i think is the hard part | 20:04 |
| fenn | in a distributed amorphous system, i dont have to worry about attaching to a specific electrode | 20:04 |
| fenn | i just have to make sure the floaty things are all stuck together | 20:05 |
| genehacker_ | cool | 20:05 |
| fenn | and it's all bio-producible | 20:05 |
| kanzure | maybe I should just go read up on protein engineering | 20:05 |
| fenn | i mean, why would you want electrodes? | 20:05 |
| genehacker_ | I just saw some mammatus clouds | 20:06 |
| genehacker_ | electrodes? | 20:06 |
| genehacker_ | are you talking about the method of microarray DNA synthesis that uses electrodes | 20:06 |
| kanzure | the azobenzene light-gated ion channels are a nice example | 20:06 |
| kanzure | maybe a polymerase that has different tunnels for different nucleotides in the template would be ideal? | 20:07 |
| kanzure | no | 20:07 |
| kanzure | this is fenn's super writozyme thingy | 20:07 |
| fenn | genehacker_: yes pretty much.. but the electrodes are the easy part | 20:07 |
| fenn | and i think it'd work better without them anyway | 20:07 |
| splicer | as I remember it inside the polymerase there is a mechanism that reads the nucleotides a bit ahead of time... so it's this mechanism that has to be modified? | 20:07 |
| kanzure | fenn: be careful, the electrode methods are a different technique and might be what genehacker_ is talking about .. i.e., the methods that use acids | 20:08 |
| kanzure | er, the acid/electrolysis versions or something | 20:08 |
| fenn | oh | 20:08 |
| fenn | well it woulnt be the first time he misunderstood | 20:08 |
| genehacker_ | there was a company made that uses some crazy electrochemical chips to synthesize DNA microarrays | 20:08 |
| kanzure | see? | 20:08 |
| genehacker_ | chips full DNA strands | 20:08 |
| fenn | kanzure: are there well understood (simple) models of protein conformational change in response to voltage/current? | 20:09 |
| kanzure | fenn: voltage-gated ion channels? | 20:09 |
| kanzure | I should hit you over the head for asking that question | 20:09 |
| kanzure | :p | 20:09 |
| fenn | wtf? ion channel isnt a conformational change | 20:10 |
| kanzure | oh | 20:10 |
| kanzure | er | 20:10 |
| kanzure | it opens and closes in response to voltage. the closing is a blocking phenomena | 20:10 |
| fenn | hrmm | 20:10 |
| fenn | what makes it move? | 20:10 |
| kanzure | hold on, doing some sys admin bullshit for the sata lab | 20:10 |
| genehacker_ | oh shoot | 20:11 |
| genehacker_ | I need to sign up for that | 20:11 |
| fenn | splicer: i'm getting polymerase confused with old CNC paper tape machinery now.. are you sure there is readahead in polymerase? | 20:11 |
| splicer | fenn: i'll check... i think there has to be | 20:12 |
| splicer | in the back of my head i think it was like 10bases.. i'll check | 20:13 |
| genehacker_ | wouldn't a paper tape driven CNC be a ribosome? | 20:13 |
| fenn | splicer: are you thinking of the mismatch detection? | 20:13 |
| fenn | there's both pre- and post- error correction | 20:14 |
| splicer | mmm.. yeah, | 20:14 |
| splicer | i think you're right | 20:14 |
| fenn | as far as i know the actual 'swap' occurs in one step | 20:14 |
| fenn | there's no RAM or anything | 20:14 |
| fenn | after all what would be the point? | 20:14 |
| kanzure | http://heybryan.org/school/buildingbrains/all.html | 20:14 |
| kanzure | search for "voltage-gated" or "voltage gated" | 20:14 |
| kanzure | er | 20:15 |
| kanzure | or just 'voltage' | 20:15 |
| kanzure | "voltage dependent ion gates" | 20:15 |
| fenn | the neuron stuff? dont they work on rate of change anyway? | 20:16 |
| kanzure | http://en.wikipedia.org/wiki/Voltage-gated_ion_channel | 20:17 |
| kanzure | ah, "voltage drop" | 20:17 |
| kanzure | "it is possible to surmise that when a potential difference is introduced over the membrane, the associated electromagnetic field induces a conformational change in the potassium channel." | 20:17 |
| kanzure | "The conformational change distorts the shape of the channel proteins sufficiently such that the cavity, or channel, opens to admit ion influx or efflux to occur across the membrane, down its electrochemical gradient. This subsequently generates an electrical current sufficient to depolarise the cell membrane." | 20:18 |
| fenn | sorry kanzure but your massive page of transcribed professor speak doesn't really help | 20:18 |
| kanzure | the wikipedia article is better :) | 20:18 |
| fenn | as usual | 20:18 |
| kanzure | The voltage-sensitive protein domain of these channels (the "voltage sensor") generally contains a region composed of S3b and S4 helices, known as the "paddle" due to its shape, which appears to be a conserved sequence, interchangeable across a wide variety of cells and species. Genetic engineering of the paddle region from a species of volcano-dwelling archaebacteria into rat brain potassium channels results in a fully functional | 20:18 |
| kanzure | huh | 20:18 |
| kanzure | there's a domain that is voltage-sensitive. how convenient. | 20:18 |
| fenn | are you sure it's voltage sensitive or actually just ion flow sensitive? | 20:19 |
| fenn | because without a membrane you have no ion flow | 20:19 |
| kanzure | this article is lacking references | 20:19 |
| kanzure | someone deserves to die | 20:19 |
| fenn | meh | 20:20 |
| fenn | i can see how that would work | 20:21 |
| fenn | it could be like a snap action switch, or like a pressure regulator | 20:21 |
| fenn | anyway if my thinking is right it's based on osmotic pressure | 20:22 |
| fenn | or whatever the voltage-iduced equivalet is | 20:22 |
| fenn | great now my n key is doig it too | 20:22 |
| fenn | h seems to be better, maybe the thig just moved | 20:23 |
| * fenn shakes laptop upside dow vigorously | 20:23 | |
| genehacker_ | hmmm... | 20:23 |
| genehacker_ | looks like It's going to hail here | 20:23 |
| fenn | duck and cover | 20:24 |
| fenn | one of these days we'll have a super duper protein simulation environment and we can just load the pdb and watch it go | 20:26 |
| fenn | like nanorex | 20:26 |
| splicer | fenn: I think you were right about the mismatch repair .. thanks. | 20:26 |
| fenn | then i won't have to bother with whatever the protein scientists are calling it, i can just see how the damn thing works | 20:27 |
| -!- splicer is now known as splicer_zzz | 20:28 | |
| fenn | splicer_zzz: what does sthf stand for? | 20:28 |
| genehacker_ | sigh | 20:28 |
| fenn | swedish transhumanist front? | 20:28 |
| * kanzure goes off to look for some food | 20:29 | |
| genehacker_ | If we only had ridiculous quantum computers | 20:29 |
| fenn | if only google would stop substituing every fucking letter in my search query | 20:30 |
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| kanzure | *** - NTH: (1) is not a non-negative integer | 21:08 |
| kanzure | roar! | 21:08 |
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| kanzure | car of (1) is apparently a non-negative integer, however. | 21:27 |
| kanzure | gee, that only took 20 minutes! at this rate, | 21:27 |
| * kanzure counts | 21:27 | |
| kanzure | it will only take forever. | 21:27 |
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| fenn | (forever) is not a non-negative integer | 21:45 |
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| kanzure | #:TEETH_GOAL is not a string | 21:58 |
| kanzure | hrm. | 21:58 |
| drazak_ | ugh | 22:07 |
| drazak_ | tomorrow I need to find papers to support my idea | 22:08 |
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| kanzure | drazak_: have you considered doing it the other way around? | 22:09 |
| drazak_ | no :P | 22:10 |
| drazak_ | I already emailed the ide | 22:10 |
| drazak_ | atomorrow I need to email a bibliography to support it | 22:10 |
| kanzure | maybe you should download the microfluidics paper archive in the mean time | 22:10 |
| drazak_ | got a link that I can wget? | 22:11 |
| * drazak_ going to bed | 22:12 | |
| drazak_ | couple more trivia questions | 22:12 |
| kanzure | http://heybryan.org/books/papers/microfluidics/ | 22:14 |
| kanzure | there should be a zip file in there | 22:14 |
| kanzure | or in the parent directory with an obvious name | 22:14 |
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| kanzure | "high voltage awareness day" | 22:27 |
| kanzure | sounds like a job for 4hv.org | 22:28 |
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| kanzure | *** - APPLY: argument (LAMBDA (A) (COND (A (LIST '/ A 'TEETH_GOAL)))) is not a function. | 22:43 |
| kanzure | oh, I guess I should take the car of that | 22:43 |
| kanzure | blah | 22:44 |
| fenn | kanzure: you know.. maybe you should do some basic lisp exercises first, instead of pretending you know the language | 22:48 |
| kanzure | if you stare at the code long enough, you somehow know it. | 22:49 |
| fenn | or think you know it | 22:50 |
| fenn | anyway.. what does sata want to do at the fab? | 22:50 |
| kanzure | store stuff | 22:50 |
| kanzure | and maybe do some wetlab stuff | 22:50 |
| fenn | store as in hands-off or we-can-use-it | 22:51 |
| kanzure | you know what, I'm not sure | 22:51 |
| kanzure | he would probably be ok with sharing tools, but I doubt he'd be ok with sharing materials | 22:51 |
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| fenn | storing materials? like chemicals? | 22:52 |
| kanzure | I'm talking out of my ass | 22:52 |
| kanzure | I forget what he was thinking of doing | 22:52 |
| fenn | ok. i have no idea what sata does or wants to do | 22:53 |
| kanzure | it's also kind of strange because he already has more than enough lab space | 22:53 |
| fenn | right | 22:53 |
| kanzure | one of the labs is a typical bio lab and classes go on in there | 22:53 |
| kanzure | the other lab is a concrete attachment to the greenhouse on the sixth floor of another building | 22:53 |
| kanzure | the greenhouse houses a few rooms, one of the rooms has our 400 gallon algae tanks | 22:53 |
| kanzure | s/tanks/ponds/ | 22:53 |
| kanzure | we also have some field acerage at brakenridge field labs | 22:55 |
| kanzure | maybe he's interested in using it for development of some equipment. that would make sense. | 22:55 |
| fenn | what does that mean exactly? | 22:55 |
| kanzure | that means we struck ground a week ago with some machinery to dig up space for some ponds | 22:56 |
| kanzure | 20x20x3 or so | 22:56 |
| fenn | aha | 22:56 |
| fenn | i want to do some aerostat/kite stuff in the indefinite future | 22:56 |
| kanzure | random? | 22:56 |
| fenn | for bouncing wifi or APRS around | 22:57 |
| genehacker | heh class | 22:57 |
| genehacker | es | 22:57 |
| genehacker | I learned that the hard way | 22:57 |
| kanzure | no, I mean, did we just change subjects somehow? | 22:57 |
| kanzure | by seeing them in there? | 22:57 |
| genehacker | there's also park that'd be great for aerostats | 22:57 |
| genehacker | yeah... | 22:57 |
| kanzure | what's so bad about that | 22:57 |
| kanzure | ? | 22:58 |
| genehacker | we got machinery? | 22:58 |
| fenn | er.. all i meant was it'd be nice to have some open area to mess with airplanes and kites and not have some park ranger try to shoot me | 22:58 |
| genehacker | graders or bulldozers? | 22:58 |
| kanzure | not sure | 22:58 |
| kanzure | I think we rented something | 22:58 |
| kanzure | or, BFL did | 22:58 |
| genehacker | no park ranger's gonna shoot you | 22:58 |
| genehacker | you have to worry about dogs though | 22:58 |
| genehacker | ] | 22:58 |
| genehacker | it's a dog park | 22:58 |
| fenn | well they might mess with my balloon | 22:58 |
| genehacker | kites already fly there | 22:58 |
| genehacker | nah dogs aren't too attracted to balloons | 22:59 |
| fenn | heh | 22:59 |
| fenn | where's this dog park? | 22:59 |
| kanzure | do you mean zilker? | 22:59 |
| genehacker | yeah | 22:59 |
| genehacker | I've seen people kite buggy around places like that | 22:59 |
| genehacker | I've seen kites in the trees there | 23:00 |
| kanzure | I've seen people in the trees there | 23:01 |
| fenn | people in the kites in the trees there? | 23:01 |
| kanzure | thought it was some sort of failed college experiment | 23:02 |
| fenn | is there some kind of austin wiki? | 23:03 |
| kanzure | not that I know of. | 23:03 |
| fenn | Connection to host www.urbanaustin.org is broken. | 23:05 |
| fenn | oh well | 23:05 |
| fenn | looks like it was up for a whole 4 months in 2007 | 23:06 |
| fenn | The goal of the Urban Austin project is to give the residents of Austin a complete, continually-updated online reference source of what's being planned and built. It will also serve as an online archive of this period of drastic change in the urban landscape, capturing images of a city in transition. | 23:06 |
| kanzure | there are some commercial thingies out there, but take it with some salt | 23:07 |
| kanzure | like austin360 | 23:07 |
| fenn | austin360 blows goats | 23:07 |
| kanzure | yep | 23:08 |
| genehacker | what is austin360? | 23:12 |
| genehacker | explain why it blows goats | 23:12 |
| kanzure | it's a terrible website | 23:13 |
| genehacker | oh yeah | 23:13 |
| genehacker | that... | 23:13 |
| fenn | it's mostly ads for TV shows, and the rest is just journalists bitching | 23:14 |
| genehacker | of course... | 23:14 |
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