| --- Day changed Wed May 27 2009 | ||
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| fenn | heh "DNA + 151 rum" | 01:28 |
|---|---|---|
| fenn | inosine is already a common additive | 01:28 |
| fenn | zoinks | 01:34 |
| fenn | '' reader One Comment | 01:34 |
| fenn | 70GB PDF library workflow on OSX | 01:34 |
| fenn | I have a 70GB library of textbooks and technical journal articles in (mostly) PDF format. | 01:34 |
| fenn | (mac cowell) | 01:34 |
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| fenn | why are microsd cards all black? you cant label the damn things so they're near impossible to tell apart | 01:36 |
| genehacker | because then you lose the card easier and have to buy more | 01:39 |
| genehacker | otherwise they'd be fluourescent yello | 01:39 |
| genehacker | I need to find some SD cards that have some pictures.... | 01:40 |
| genehacker | otherwise I have to take apart my old computer and figure out how to get the data off it... | 01:41 |
| fenn | hmm those holographic data cards were fluorescent yellow | 01:48 |
| fenn | but a micro SD card holds more | 01:49 |
| fenn | and the reader costs 10,000 times less | 01:49 |
| fenn | http://en.wikipedia.org/wiki/Holographic_Versatile_Card | 01:51 |
| fenn | OLPC display looks way better than the kindlee | 01:56 |
| fenn | hmm part of that is because it zooms properly | 01:59 |
| fenn | for "the standard" in typesetting programs, LaTeX output sure looks like crap | 02:04 |
| fenn | maybe it's just whatever font is installed as default on (all of) my computers (ever) but the text is all humpty dumpty looking, the tops and bottoms are not at the same height and the letters are different size | 02:05 |
| genehacker | I think I need to read more about the connection machine | 02:05 |
| genehacker | heck I think I saw one of them... | 02:05 |
| fenn | i think it's "computer modern" that looks the worst | 02:07 |
| fenn | genehacker: big black box with red led's all over the front | 02:08 |
| fenn | "you can't miss it" | 02:08 |
| genehacker | oh it was just the frame it seems | 02:09 |
| fenn | quite possibly the sexiest computer case ever designed | 02:09 |
| genehacker | http://www.computerhistory.org/collections/accession/102691297 | 02:09 |
| genehacker | no the sexiest computer case ever designed was this one supercomputer | 02:09 |
| fenn | OH YEAH? | 02:09 |
| genehacker | it glowed red | 02:10 |
| genehacker | it looked like a casemod, a casemod as tall as a man | 02:10 |
| genehacker | yikes, space could be a whole bunch of randomly connected cellular automata | 02:11 |
| fenn | now you must do penance by bashing your forehead with "new kind of science" ten thousand times a day | 02:12 |
| genehacker | no I don't think you get it | 02:13 |
| fenn | why not? | 02:13 |
| genehacker | my worst worry is that the universe is a screensaver | 02:13 |
| fenn | i was thinking about this stuff before you were born :P | 02:13 |
| genehacker | heck I've ran cellular automata that form random networks capable of sustaining waveforms | 02:15 |
| fenn | about the only response i got was "well, so what if it is?" from my brother | 02:15 |
| fenn | you have? | 02:15 |
| genehacker | I just hope I didn't make anything sentient | 02:15 |
| genehacker | well this is probably just crazy talk | 02:15 |
| fenn | i'm going to have to arrest you for violating intergalactic law | 02:15 |
| fenn | hold still while we send a ship to your dimension | 02:16 |
| genehacker | well then how do I know you didn't accidently create a sentient entity just by breathing? | 02:16 |
| genehacker | I think I need to read more about cellular automata | 02:17 |
| genehacker | damn fluid dynamics just using cubes that can move in 6 directions | 02:18 |
| genehacker | heh I wonder If I could implement that in DNA... | 02:19 |
| fenn | can cubes move in more than 6 directions? | 02:22 |
| genehacker | http://www.cs.washington.edu/homes/ruzzo/ | 02:23 |
| genehacker | not here | 02:23 |
| fenn | hmm i wonder why max used the word 'ultrahuman' instead of just 'superhuman' | 02:25 |
| fenn | doesn't ultra mean "last"? | 02:25 |
| genehacker | because it is ultraawesome | 02:26 |
| fenn | it should have been "hyperhuman" following the typical progression of superlatives | 02:26 |
| fenn | am i supposed to trademark that now? | 02:26 |
| fenn | genehacker: how much truth behind that page? | 02:28 |
| genehacker | none unfortunately | 02:30 |
| fenn | no time cube for you then | 02:33 |
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| fenn | huh. danny hillis from 1998 "Why Y2K (as we experts so fondly call it) instead of bio-terrorism, the next energy crisis, or the return of the swine flu?" | 03:22 |
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| kanzure | "I'm Mac Cowell. I'm an amateur biologist. I'm a part of diybio.org. I'm interested in synthetic biology and refactoring and reengineering existing biological tools and techniques to be cheaper, easier, safer, and more accessible." | 07:31 |
| kanzure | sounds untrue :/ | 07:31 |
| kanzure | oh well. /me goes off to hop a bus. | 07:32 |
| kanzure | fenn: joseph jackson seems to think that he's on a "social mission" more than anything else. | 07:39 |
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| splicer | fenn: sthf = Svenska TransHumanistForbundet, The swedish transhumanist association | 08:24 |
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| kanzure | "Reminds me of those experiments that we used to to do in cub scouts - sticking a small mirror onto a thin sheet of clingfilm and watching how sound waves changed the direction of reflected light - to demonstrate how sound was just air moving rapidly." | 10:35 |
| * drazak_ never heard of that | 10:40 | |
| kanzure | isn't that a photo-microphone or something? | 10:41 |
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| kanzure | http://openwetware.org/index.php?title=DIYbio/FAQ&action=history | 11:08 |
| kanzure | why doesn't anybody else edit it | 11:08 |
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| kanzure | not bad | 11:37 |
| kanzure | http://en.wikipedia.org/wiki/Oligonucleotide_synthesis | 11:37 |
| kanzure | I think it's time to re-read wikipedia | 11:37 |
| kanzure | yay it has references | 11:37 |
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| kanzure | ^ Schulhof, J. C.; Molko, D.; Teoule, R. The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups. Nucleic Acids Res. 1987, 15, 397-416. | 11:44 |
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| kanzure | synthetic neurobiology "labcast" from ed boyden: http://labcast.media.mit.edu/?p=63 | 12:16 |
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| ultraleibniz | I like this. | 12:50 |
| ultraleibniz | http://heybryan.org/books/papers/www/biochem118/cmgm.stanford.edu/biochem118/Papers/ | 12:50 |
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| drazak_ | "Jim in four weeks created the GigAssembler by working night and day," | 13:07 |
| drazak_ | Dr. Haussler said. "He had to ice his wrists at night because of the | 13:07 |
| drazak_ | fury with which he created this extraordinarily complex piece of | 13:07 |
| drazak_ | code." | 13:07 |
| katsmeow-afk | the odor of overheated flesh permeated the dorm facility..... | 13:11 |
| xp_prg | kanzure I don't understand why it is hard to assemble dna | 13:11 |
| katsmeow-afk | the devoted gathered is quiet adoration outside in the fields..... | 13:11 |
| katsmeow-afk | his Name was spoken in respectfully hushed tones, with bowed heads..... | 13:12 |
| * katsmeow-afk stops now | 13:13 | |
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| kanzure | xp_prg: it's more of an art of statistis | 13:26 |
| kanzure | *statistics | 13:26 |
| kanzure | because you need to find overlapping pieces of the genome | 13:26 |
| kanzure | you don't sequence everything linearly, you sequence it in chunks | 13:26 |
| kanzure | and those chunks are out of order | 13:26 |
| drazak_ | katsmeow-afk: that was muad'dib, not Jim | 13:30 |
| drazak_ | :P | 13:30 |
| kanzure | his Name was spoken in respectfully hushed tones, with bowed heads... | 13:32 |
| kanzure | At long last the creator spoke and he was vastened, and all was Good. | 13:32 |
| kanzure | 'Avast, ye heathen mortals! There be code to conquer, sources to write.' | 13:33 |
| kanzure | And the good people mumbled amongst themselves in awe, for before them stood a God-- | 13:33 |
| kanzure | vanishing, He left them all with the most beautiful program ever | 13:33 |
| kanzure | written completely in LISP and without comments and was consequently utterly useless. | 13:34 |
| kanzure | a big thanks goes to the big G. | 13:34 |
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| kanzure | er, so, even in my dreams I'm promising people I'll write code for them for different things | 14:12 |
| kanzure | the latest one was something about writing a hierarchical tournament generator thingy for a coach | 14:12 |
| kanzure | "you're doing that by hand? gah!" | 14:13 |
| drazak_ | you, my friend, need to meet randall monroe | 14:18 |
| kanzure | have you met him? | 14:47 |
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| genehacker | kanzure? | 15:40 |
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| kanzure | genehacker: yes? | 16:00 |
| kanzure | genehacker: do you have all of the references to KSRM yet? | 16:08 |
| kanzure | fenn: how many metalworking/machine shops would you estimate have an actual inventory list? have you seen enough to get a good bayesian clue? | 16:12 |
| kanzure | I'm thinking of telling [AustinMetalworkingEtc] that if any of them buy me lunch, I'll type up their inventory for them :p | 16:13 |
| kanzure | huh? | 16:29 |
| kanzure | http://en.wikipedia.org/wiki/Single_Molecule_Real_Time_Sequencing | 16:29 |
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| kanzure | "The ZMW holes are ~70nm in diameter and ~100nm in depth. Due to the behavior of light when it travels through a small aperture, the optical field decays exponentially inside the chamber [4]. The observation volume within an illuminated ZMW is ~20 zeptoliters (20 X 10-21 liters). Within this volume, the activity of DNA polymerase incorporating a single nucleotide can be readily detected." | 16:43 |
| kanzure | http://heybryan.org/books/papers/craighead/ for the papers & wikipedia article | 16:43 |
| kanzure | er, that was 1E-21 not 10-21 | 16:44 |
| kanzure | fenn: sounds like they have to immobilize the polymerase | 16:46 |
| genehacker | references? | 16:46 |
| genehacker | huh? | 16:46 |
| kanzure | genehacker: in the link | 16:46 |
| kanzure | http://heybryan.org/books/papers/craighead/ for the papers & wikipedia article | 16:46 |
| kanzure | "The Pacific BiosciencesTM company expects to commercialize the SMRTTM sequencing in 2010 or 2011. The prototype of the SMRTTM chip contains 1000 ZMW holes that allow the parallelized DNA sequencing. Each of the ZMW holes produces approximately 1,500 bp(base pair)in read lengths at the speed of 10 bp per second." | 16:46 |
| kanzure | I wonder if we could make it before 2010 | 16:46 |
| genehacker | me two | 16:48 |
| genehacker | I wonder if we could sell it in china or some place with lax copyright laws like hong kong | 16:48 |
| kanzure | you mean patent laws | 16:48 |
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| kanzure | what was the size of the smallest genome again? something like 600 kbp ? | 16:51 |
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| kanzure | 580,073 | 16:53 |
| genehacker | is the size of the mycoplasma genitalium genome | 16:56 |
| genehacker | in BP | 16:56 |
| kanzure | http://en.wikipedia.org/wiki/Talk:Single_Molecule_Real_Time_Sequencing#Application | 16:58 |
| kanzure | made a comment about the fishyness of it all | 16:58 |
| genehacker | so what were you saying before 580,073/ | 17:01 |
| kanzure | was just trying to remember the size of the genome of mycoplasma genitalium | 17:02 |
| kanzure | because I was pointing out that it's the only whole genome that the ZWT method or whatever could sequence with 1k holes, 10 bp/sec, and 1.5k bp max per hole. | 17:02 |
| fenn | 1000 holes seems too few | 17:10 |
| kanzure | "while you're down there", you might as well bother with some more | 17:10 |
| fenn | katsmeow-afk: http://www.engadget.com/2009/04/15/solaren-corp-to-supply-california-with-space-based-solar-power/ | 17:10 |
| fenn | katsmeow-afk: note the use of concentrating mirrors on solar cells | 17:11 |
| fenn | of course i'm highly skeptical it will actually get built | 17:11 |
| kanzure | hm. so it turns out that time warner cable doesn't like me hosting stuff on my servers. | 17:14 |
| kanzure | I have a 2 second lag in this channel, and I can hardly load anything over the web | 17:14 |
| kanzure | and I think people are downloading the low-cost PCR book from the server | 17:15 |
| fenn | yep | 17:16 |
| fenn | it seems they just degrade your connection rather than doing something sensible like traffic shaping | 17:16 |
| kanzure | they do that for torrenting methinks | 17:17 |
| fenn | that = what? | 17:17 |
| kanzure | although I'm not sure, a lot of this is me guessing and having bad experiences | 17:17 |
| kanzure | shaping for torrenting | 17:17 |
| fenn | yeah probably | 17:17 |
| kanzure | although it might only be for specific ports for torrenting, I haven't tried using some other port yet | 17:17 |
| fenn | anyway, it shouldn't ever drop 70% of packets to google.com | 17:17 |
| fenn | try encrypted torrent packets only | 17:17 |
| fenn | random port + encryption whee.. its like war games | 17:18 |
| kanzure | oh, I know what I'll do | 17:18 |
| kanzure | I'll host it on the sata server | 17:18 |
| fenn | no wai! | 17:18 |
| kanzure | it's not like he's not already doing something quasi-illegal by using campus resources to host the server | 17:18 |
| fenn | how is that illegal? | 17:18 |
| fenn | or even quasi-illegal | 17:18 |
| kanzure | using campus resources for a business? | 17:18 |
| kanzure | isn't that a no-no? | 17:18 |
| fenn | i thought that was the whole point :3 | 17:19 |
| fenn | see 'the kept university' etc etc | 17:19 |
| kanzure | nah, uni just sells out poor souls to already established corporations | 17:19 |
| kanzure | anyway | 17:19 |
| * kanzure goes to look at the ZWT papers | 17:20 | |
| kanzure | wonder if an immobilized polymerase can be used to electrically detect whether or not it has a certain base in its complementary hole | 17:21 |
| kanzure | er | 17:21 |
| kanzure | how is it that after all of this time I still do not know the precise mechanisms of DNA pol enzymes? | 17:21 |
| fenn | it seems 'sim city' has more influence on public policy than decades of space energy research | 17:21 |
| * fenn mumbles something about arcologies | 17:22 | |
| kanzure | fenn: btw, I'm willing to make a deal with you | 17:22 |
| fenn | let's make a deal! | 17:22 |
| kanzure | if you stop pretending to know japanese, I'll stop pretending to know LISP | 17:22 |
| kanzure | :p | 17:22 |
| fenn | well at least i dont bother you with japanese on a daily basis | 17:22 |
| kanzure | would you? | 17:22 |
| fenn | um, no | 17:22 |
| kanzure | what? | 17:23 |
| fenn | huh? | 17:23 |
| kanzure | do you or do you not bother with japanese on a daily basis? | 17:23 |
| fenn | do not | 17:23 |
| kanzure | fooey | 17:23 |
| fenn | genehacker: here's your homework for today http://www.longnow.org/views/essays/articles/ArtCompSciNG.php | 17:24 |
| genehacker | fenn I'm sewing right now | 17:25 |
| kanzure | hm. maybe I knew how polymerase truly worked at one point? | 17:27 |
| kanzure | http://heybryan.org/mediawiki/index.php/Polymerase | 17:27 |
| kanzure | "A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis" | 17:27 |
| genehacker | btw how do immune cells synthesize different antibodies? | 17:28 |
| kanzure | I would suggest picking up a good immunology book from the server :p | 17:29 |
| fenn | sequence shuffling | 17:30 |
| fenn | it's really quite interesting' | 17:30 |
| genehacker | http://www.ncbi.nlm.nih.gov/protein/193885324?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum | 17:33 |
| genehacker | how does that immunotherapy stuff work? | 17:33 |
| genehacker | that seems like it would be a rather handy sequence to know... | 17:37 |
| genehacker | delightful | 17:37 |
| genehacker | opera has flash failure too | 17:37 |
| kanzure | what did you do? | 17:37 |
| fenn | i love how in that story craig venter gives the professor (haussler) all the credit | 17:38 |
| genehacker | so if we synthesize and boot up the mycoplasma laboratorium genome... | 17:39 |
| genehacker | will he give us credit for making the first synthetic lifeform | 17:39 |
| fenn | no | 17:39 |
| fenn | the point was that the professor didnt do anything, it was his mysterious grad student | 17:40 |
| genehacker | oh | 17:41 |
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| kanzure | um, why aren't we folding proteins around nanowire support thingies | 17:44 |
| kanzure | around scaffolds | 17:45 |
| genehacker | ??? | 17:45 |
| kanzure | you'd be able to stuff stuff inside of the proteins and try to evolve for preserved functionality or something | 17:45 |
| kanzure | the stuff inside could be something like a sensor for activity | 17:45 |
| kanzure | (particularly useful for immobilization (I'm not going to consider stuffing a nanoradio into them) | 17:46 |
| kanzure | ) | 17:46 |
| kanzure | gah! I fail at lisp | 17:46 |
| kanzure | (immobilization useful particularly (nanoradio stuffing (nil consider)) | 17:46 |
| fenn | stuff! | 17:50 |
| fenn | are you talking about hybrid organic/inorganic tiles? | 17:50 |
| kanzure | tiles? | 17:50 |
| fenn | remember the DNA FPGA thing? | 17:51 |
| kanzure | no, I was thinking of putting wires in between the giant wrapping helices in proteins | 17:51 |
| kanzure | no, not really | 17:51 |
| kanzure | I mean, no, I do not mean that | 17:51 |
| fenn | those ribbon diagrams are not entirely representative of reality | 17:51 |
| kanzure | right, but proteins are also not wall-to-wall or the volume entirely filled with super-compressed material | 17:52 |
| fenn | they're pretty well stuffed | 17:52 |
| fenn | i mean, illustrations in textbooks are all way too sparse | 17:52 |
| fenn | as far as protein concentration goes at least | 17:53 |
| fenn | sorry i derailed your idea | 17:54 |
| kanzure | nah, it's not a problem | 17:54 |
| kanzure | I'm still reading up on polymerase conformational changes anyway | 17:54 |
| fenn | i think the phrase you're looking for is "inorganic ligand" | 17:55 |
| genehacker | nanoradios are cool | 17:55 |
| kanzure | most ligands that I've heard about are usually external | 17:55 |
| fenn | they're usually at the active site, because it's what makes the reaction go | 17:56 |
| fenn | like a dill bit | 17:56 |
| fenn | drill bit* | 17:56 |
| kanzure | "'reveals a major conformational change wherein the C-terminal subdomain of the polymerase rotates around the axis of -helix M to produce main chain movements of up to 11 Å . This substrate-induced subdomain closure around the DNA is accompanied by numerous smaller (1−2 Å;) conformational changes " | 17:56 |
| fenn | fantastic | 17:56 |
| fenn | an animated 3d model would be infinitely better | 17:57 |
| kanzure | there's a link, but it's broken | 17:57 |
| kanzure | http://www.chem.ucsd.edu/Faculty/Kraut/bpol.html | 17:57 |
| fenn | roadrunner hates me | 17:58 |
| kanzure | the trick is to hate roadrunner more than it hates you | 17:58 |
| kanzure | yay google | 18:01 |
| kanzure | http://chem-faculty.ucsd.edu/kraut/bpol.html | 18:01 |
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| kanzure | "If you have any comments about my movie, please click on my email Michael R. Sawaya (msawaya@ucsd.edu). A screen should pop up (its self explanatory) and there will be buttons for send and cancel. " | 18:07 |
| kanzure | wtf | 18:07 |
| kanzure | how ancient is this? | 18:07 |
| kanzure | "The terminal base pair of the primer stem forms the other side of this pocket, which has a geometry that precisely accommodates a correct base pair." | 18:10 |
| kanzure | that sounds unlikely | 18:10 |
| kanzure | "Again, this binding pocket is defined by both nucleic acid and protein, that is, the correct 3'-terminal base pair forms one side of the pocket and specific amino acids in the O helix form the other side. The resulting slot provides a tight fit (Fig. 2) for incorporation of the correct nucleotide. " | 18:11 |
| fenn | why does it sound unlikely? | 18:17 |
| kanzure | because incorrect base pairs are sometimes incorporated | 18:17 |
| fenn | if it werent a tight fit, you'd get the wrong base 75% of the time | 18:18 |
| fenn | you have to remember everything is constantly being battered with the equivalent of a tidal wave full of sledgehammers | 18:19 |
| fenn | so the shape of the protein is not always exact | 18:19 |
| fenn | i wonder why i havent heard about freedomofscience.org yet | 18:20 |
| kanzure | really? | 18:21 |
| kanzure | it's why joseph bothered to meet up with me | 18:21 |
| kanzure | "One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a “short-term memory” of replication errors, responding to mismatches up to four base pairs in from the primer terminus" | 18:22 |
| fenn | i thought you already knew him from WTA | 18:23 |
| kanzure | no, not at all | 18:23 |
| fenn | is he not the transhumanist bodybuilder guy? | 18:23 |
| kanzure | it just so happens that he's well acquainted with the WTA folks | 18:23 |
| kanzure | he is | 18:23 |
| kanzure | but it's more of a "just so happens" rather than me meeting him through those channels | 18:23 |
| genehacker | he might give bodybuilder a whole new meaning | 18:24 |
| kanzure | apparently he graduated from harvard | 18:25 |
| kanzure | he's a bit on the obsessive side :) | 18:25 |
| fenn | "Using a Wiki format, we will create a community annotation system to review licenses and say whether they share." | 18:29 |
| fenn | yarr | 18:29 |
| kanzure | fenn: can you be over here by 4:30 tomorrow? | 18:30 |
| fenn | any kind of data aggregation/standardization is better than none | 18:30 |
| kanzure | Andrew and Linda are visiting, and she wants to meet you and such | 18:30 |
| fenn | 4:30 i'll set my alarm | 18:30 |
| kanzure | for 4:30 ? | 18:30 |
| fenn | heh' | 18:30 |
| fenn | i'm so fucked | 18:30 |
| fenn | (just in general) | 18:31 |
| genehacker | damn | 18:31 |
| kanzure | good luck with that | 18:31 |
| genehacker | I'm stuck down here | 18:31 |
| kanzure | fenn: would pushing it out be better? | 18:31 |
| kanzure | because she just offered to get me some food tomorrow, so if we shop first that's ideal | 18:31 |
| fenn | hmm ok i guess | 18:32 |
| fenn | genehacker: where are you stuck? | 18:32 |
| genehacker | where I am right now | 18:32 |
| genehacker | home | 18:32 |
| genehacker | sewing up a thermal management garment | 18:33 |
| fenn | i see. do you need someone to call emergency services to come extract you? | 18:33 |
| genehacker | no | 18:33 |
| fenn | ok :) | 18:33 |
| kanzure | I want to go eat somewhere tonight | 18:33 |
| genehacker | It's just a big hassle to get to austin from here | 18:33 |
| kanzure | maybe freebirds. anybody want to come? | 18:33 |
| genehacker | I could use a teleporter or at least some telepresence gear | 18:34 |
| kanzure | er, you mean a webcam? | 18:34 |
| genehacker | no I mean claytronic doubles | 18:34 |
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| fenn | can you eat claytronics? | 18:35 |
| genehacker | or a robot with stereovision, manipulators, and a low latency internet connection | 18:35 |
| genehacker | yes, but it isn't good for you | 18:35 |
| fenn | kanzure: i'm available tonight, as usual | 18:37 |
| kanzure | well if you want to eat feel free to come over I guess | 18:40 |
| xp_prg | ok kanzure thanks | 18:43 |
| kanzure | xp_prg: ? | 18:43 |
| xp_prg | over for dinner | 18:43 |
| kanzure | aren't you on the wrong side of the continent? | 18:44 |
| xp_prg | ya | 18:44 |
| xp_prg | :) | 18:44 |
| fenn | mail me some claytronics | 18:44 |
| fenn | or a crossed atom beam hologram | 18:44 |
| genehacker | or telepresence robot | 18:45 |
| fenn | i'm telling you, you can't eat a telepresence robot | 18:45 |
| genehacker | woo 12 feet out of 25 feet done | 18:45 |
| genehacker | time to get moar supplies | 18:45 |
| kanzure | fenn: sometime soon or I'll go without you :p | 18:46 |
| fenn | oh, right | 18:46 |
| genehacker | man one of those autonomous flying delivery vehicles would be great right about now... | 18:46 |
| fenn | brt | 18:46 |
| kanzure | "The polymerase, therefore, plays a significant role in defining the local and the global conformation of each mismatch structure. The type and degree of disruptions at the polymerase active site vary depending on the identity of the mismatch. We present our observations categorized by the type of base pairing." | 18:49 |
| kanzure | nice, it has a list of different conformational changes for each type of mismatch incorporation | 18:51 |
| kanzure | now if only it would do that for correct pairings :p | 18:52 |
| genehacker | whoa | 18:59 |
| genehacker | solaren says they're gonna get 4.8 gigawatts from space solar? | 19:00 |
| genehacker | that is a lot | 19:00 |
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| kanzure | photosensors that induce conformational changes: Vivid, White Collar-1, ZEITLUPE, ENVOY, and flavin-binding, kelch repeat, F-BOX 1 (FKF1) | 23:26 |
| kanzure | flavin-based photosensors, | 23:29 |
| kanzure | comprising cryptochromes, LOV (Light, Oxygen and Voltage) and BLUF (Blue Light | 23:29 |
| kanzure | sensing Using FAD) protein | 23:29 |
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