| --- Log opened Thu Dec 01 00:00:07 2011 | ||
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| JayDugger | Good morning, everyone. | 07:22 |
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| strages | morning | 08:50 |
| kanzure | hi | 08:53 |
| delinquentme | OMG | 08:53 |
| delinquentme | CHECK IT GUYS | 08:53 |
| delinquentme | I GOT A SMERT ANSAR | 08:53 |
| delinquentme | http://www.reddit.com/r/askscience/comments/mvhrz/what_is_the_limiting_factor_in_dna_sequencing/#c3485bf | 08:53 |
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| kanzure | delinquentme: those are some pretty standard answers | 08:56 |
| kanzure | wait, 5000 bps? | 08:57 |
| delinquentme | 5k is the top best of the best we can do | 08:58 |
| delinquentme | dont quote me | 08:58 |
| delinquentme | kanz im looking for this article talking about using microfluidics for chromosomal separation pre sequencing | 08:59 |
| delinquentme | any idea where it might be? | 08:59 |
| kanzure | do you mean sorting of chromosomes or hylicase-based separation | 09:01 |
| kanzure | helicase | 09:01 |
| kanzure | or whatever the enzyme is supposed to be; helicase isn't for chromosomes | 09:01 |
| delinquentme | umm sorting of chromosomes | 09:02 |
| delinquentme | i remember it off of fluidic mems | 09:02 |
| delinquentme | i THOUGHT | 09:02 |
| kanzure | lots of papers about sorting: http://diyhpl.us/~bryan/papers2/microfluidics/ | 09:02 |
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| hymension | home to the nodes of ranvier | 13:46 |
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| delinquentme | umm HERROH. | 13:54 |
| delinquentme | IM ROOKING far SomeWAN who knoz how to prep DNA | 13:54 |
| delinquentme | eudoxia, howdah! | 13:54 |
| delinquentme | kanzure, have you ran into any cool microfluidic setups for PCR? | 14:01 |
| kanzure | tons.. yes | 14:03 |
| kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/pcr/ | 14:04 |
| delinquentme | hot. | 14:04 |
| delinquentme | damn. | 14:04 |
| delinquentme | umm DNA quantification | 14:04 |
| kanzure | also this one | 14:04 |
| kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/gkm389%20Miniaturized%20PCR%20chips%20for%20nucleic%20acid%20amplification%20and%20analysislatest%20advances%20and%20future%20trends.pdf | 14:04 |
| kanzure | and since you're hot for dna sequencing stuff: | 14:04 |
| kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/Microfabricated%20bioprocessor%20for%20integrated%20nanoliter-scale%20Sanger%20DNA%20sequencing.pdf | 14:04 |
| kanzure | http://diyhpl.us/~bryan/papers2/microfluidics_Sanger_sequencer.png | 14:05 |
| kanzure | http://diyhpl.us/~bryan/papers2/DNA/High-fidelity%20gene%20synthesis%20by%20retrieval%20of%20sequence-verified%20DNA%20identified%20using%20high-throughput%20pyrosequencing%20-%202010.pdf | 14:05 |
| kanzure | http://diyhpl.us/~bryan/papers2/DNA/Real-time%20DNA%20sequencing%20from%20single%20polymerase%20molecules.pdf is a famous one (by now) | 14:05 |
| delinquentme | kanzure, dna quantification | 14:11 |
| delinquentme | that is the big term for separating out DNA by length right? | 14:12 |
| kanzure | uh? i don't know for sure | 14:27 |
| kanzure | sounds like BS | 14:27 |
| delinquentme | dna quantitation | 14:30 |
| delinquentme | its getting the fluid / DNA saturation just right | 14:31 |
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| Utopiah | http://www.roboticopenplatform.org | 22:30 |
| kanzure | do i have to | 22:30 |
| Utopiah | "Robotic Open Platform (ROP) aims to make hardware designs of robots available under an Open Hardware license to the entire robotic community. It provides CAD drawings, electric schemes and the required documentation to build their own robot." | 22:34 |
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| --- Log closed Fri Dec 02 00:00:09 2011 | ||
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