2012-03-10.log

--- Log opened Sat Mar 10 00:00:45 2012
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kanzurehttp://www.digital-bio.com/micro/neon.htm "A novel, benchtop electroporation device that02:31
kanzureemploys an microporation technology."02:31
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foucistmicropenis technology? gosh!03:15
kanzurelike a boss03:21
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delinquentmeI pick one door out of 10,000.  The game host removes 9,998 other doors ... leaving two doors: My initial choice door and another.  Im told the prize is behind one of these doors.  I switch to the *OTHER* door because it is more probable that that door has the prize.  the *WHY* is what im after.. and I THINK it is because unlike my initial door choice the other door does not have the initial associated probability of 1:10,06:46
delinquentme000 of being correct.06:46
delinquentmeif anyone is really good w stats :D06:46
ThomasEgithat depends06:49
ThomasEgiif the game host puts the price behind the door befor you pick yours. then chances are that the price is behind the door the computer picks.06:50
uniqanomalyhttps://en.wikipedia.org/wiki/Monty_Hall_problem06:50
ThomasEgiif the computer just picks a random door. and then puts the price behind one of the 2 remaining. it makes no difference which one you pick06:50
chris_99yay just picked up an Electrophoresis PSU for £26 :)06:52
delinquentme^^07:38
kanzurebeep boop07:38
delinquentmeThomasEgi, you're saying that if the prize door is set before you pick the initial door and it stays at that door then you should switch07:39
delinquentmeagreed.07:39
delinquentmeim just not sure if its because theres a prior probability07:39
delinquentmei *THINK* thats why07:39
delinquentmehowdy kanzbot07:39
delinquentmeveridical paradox  ???07:40
kanzuredelinquentme: pm07:42
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kanzurehmm10:16
kanzurehttp://gen9bio.com/10:16
kanzure"A small number of Gen9’s current production tools generate sufficient DNA content to, in principal, replace the world’s current annual output of synthetic genes."10:16
kanzure"based on next generation gene synthesis technologies."10:16
chris_99know what tech. they use?10:17
kanzureprobably ligation10:17
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delinquentmekanzure, you mentioned getting the average length of all genes which exist in the online repositories right?10:35
kanzureuh sure10:37
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delinquentmeyeah that it would be a comparison worth pursuing right?10:50
delinquentmehowd the pitch go?10:50
kanzurewent well10:58
kanzuremaradydd is telling me that IDT *builds* its own DNA synths10:59
kanzurehttp://eu.idtdna.com/pages/home/about-us/what-we-do10:59
kanzure"DT manufactures the oligo using chemical DNA synthesis machines designed and built in-house."10:59
kanzurethey found it was cheaper to just hire some dude to build synths10:59
kanzurethan to buy synths on the market10:59
delinquentmewith what equipment?11:00
kanzurewhat?11:00
delinquentmei like arguing too much11:02
delinquentmehow does that individual build the oligos11:03
kanzurehe makes the machines11:03
kanzurenot the oligos11:03
delinquentmeoh11:03
delinquentmefuck11:03
delinquentmehaha11:03
delinquentmewhos this dude?11:03
kanzurenobody in particular- i think it might be a team11:03
kanzureprobably just some engineer11:03
kanzurenothing special.. just told to make synthesizers for the company11:03
delinquentmePS i still dont have a good answer for a web server stack to power the backend of a networked open hardware system11:04
delinquentmeam i totally bonkers for saying " fuck it " and building it w rails?11:05
delinquentmei see no reason not to11:05
kanzureyou are totally bonkers yes11:06
delinquentmewhy :D11:06
delinquentmeis the question11:06
delinquentmei mean in *this* instance11:06
delinquentmei know why in general11:06
kanzurewhy does it need to be a web server?11:07
delinquentmebc i want it networked11:08
delinquentmeand thats my hammer11:08
delinquentmeim in startups trolling as well11:08
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delinquentmeso if the majority of the server processing im going to be doing is going to be protocols11:14
delinquentmei definitely need something event based11:15
delinquentmeso EM is a big part of that11:15
delinquentmenginx and thin are EM based I believe11:15
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yash-phonefucking internet11:54
Mariukanzure, what do you think of Gregory Stock ?11:55
Utopiah_http://www.wired.com/epicenter/2012/03/opinion-wolfram-life-analytics/11:56
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delinquentmejust finished chatting w a christian12:46
delinquentmesaid hes probably the smartest person on earth12:47
delinquentme#truestory12:47
ThomasEgigodfags. allways good for some saturday evening entertainment12:48
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Mariudelinquentme, what channel ?12:59
delinquentme#ai12:59
delinquentmedropped a few questions on him12:59
delinquentmei closed the chat13:00
delinquentmehow do you bring up past convos?13:00
delinquentmeor the convo history?13:00
kanzuredo you lg?13:00
kanzure*log13:00
Urchindelinquentme: it would depend on your client13:00
Mariuindeed13:06
Urchinlook up xchat's manual13:08
Urchinor dig trough the options13:08
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foucistdelinquentme: i usually just go into the terminal, find the log file, and grep it13:14
foucistor open in vim etc13:14
UrchinI usually just use less13:16
kanzurei use dd13:17
kanzurescrew all of you13:17
Urchinwtf?13:18
kanzurehaha13:19
kanzureUrchin: i am implying i use something unnecessarily ridiculous13:19
kanzureand thus it is funny or humorous13:19
kanzurealso the stormstrooper shot is pretty rad: http://i.imgur.com/1r6bl.jpg13:19
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Mariuthat's so cool, thanks kanzure13:22
delinquentmexchat is the client13:22
delinquentmelol here we go13:22
delinquentme<DanFrederiksen> intelligence is not monolithic but in terms of true value and overall combined capability I am as smart as they come. perhaps the smartest on the planet13:22
delinquentmeand *christian*13:23
delinquentmeulululuz13:23
Mariulol13:23
kanzureah another person who believes in intelligence13:26
kanzurewell i guess that's what happens in #ai13:26
Urchinol13:26
Urchinput it on fstdt13:26
Urchin*lol13:26
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delinquentmeFSDT?13:27
Urchinhttp://www.fstdt.com/13:28
Urchinofcourse: http://www.fstdt.com/Top100.aspx13:29
delinquentmeLOL13:33
delinquentmeawesome13:33
kanzureprotein engineering tutorial http://openwetware.org/wiki/20.109%28S12%29:Start-up_protein_engineering_%28Day1%2913:37
kanzure"lyse, lyse baby" http://www.seringhaus.net/lyse.html13:38
kanzureYo, PhD – let’s mix it.13:38
kanzureAlright stop, grab some yeast and a test tube,13:38
kanzureStick it in buffer that’s cold like an ice cube,13:38
kanzureNothin’ – gonna grow when you cool it,13:38
kanzureSplit the bug up, slap it on a GeneChip,13:38
kanzureWill it bind the probe? Yo – I can’t guess13:38
kanzureTurn off the lights, it’ll fluoresce13:38
kanzureThis lab, bitch, is dope delirium13:38
kanzureKnockin’ out genes in some bacterium13:38
kanzureStaph, Pseudomonas, E.coli13:39
kanzureLayin’ the pain on a mutant fruit fly13:39
kanzurePhage, suckers kill bacteria13:39
kanzureGot RNA genomes so don’t let em near ya13:39
yashgarothfffffff13:39
kanzureLove it or leave it, it ain’t DNA,13:39
kanzureBut does show up on a microarray,13:39
kanzureIf you got a genome, yo, fantastic,13:39
kanzureCheck SWISSPROT while you waitin’ for your BLAST hit13:39
kanzureyashgaroth: YOU WILL ENJOY THIS13:39
kanzureor else13:39
yashgarothmy legs, they're...moving...to the beat!13:39
kanzureYo man – Let’s get outta here. Cells to the shaker.13:39
MetaMorphkanzure, keep it... procreative... yo?13:40
delinquentmeoh man rhymed verse13:40
delinquentmeach13:40
delinquentmepainful13:40
kanzurehi MetaMorph13:41
kanzuredelinquentme: gotta know your rhymes13:41
* MetaMorph is.13:42
delinquentmekanzure, talk to me when you've lived through these times13:42
* delinquentme blows brains out13:42
delinquentmelol13:42
delinquentmekanzure, what liquid volumes are microfluidics @13:44
delinquentmenot pico liters right?13:44
kanzuresometimes picoliter13:45
kanzuresometimes femtoliters13:45
delinquentmewaaaooowww13:46
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delinquentmewell i guess size of the syringe would also help13:47
kanzurehuh?13:47
kanzurehttp://diyhpl.us/~bryan/papers2/microfluidics/Droplet%20microfluidics.pdf13:47
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delinquentmeencoder resolution <> smallest vol dispensed reliably <> syringe size13:50
delinquentmeif i need high resolution.. just use smaller syringes13:50
kanzureor you just pump liquid through at a certain pressure13:51
delinquentmewhat pressures do they run at?13:52
delinquentmeim not going to need special syringes am i?13:52
delinquentmenL pL fL yeah13:52
delinquentmekk13:52
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kanzure"Blue Heron Bio's GeneMaker® proprietary, novel and high throughput design and synthesis platform synthesizes genes and synthetic DNA sequences economically and with perfect accuracy."14:06
kanzure"Jamie Heywood or people in his group - rogue scientists who were trying to come with a cure for ALS which Heywood’s brother had."14:07
kanzure"Our first team, led by Shawn Mikula (working in the laboratory of Winfried Denk at the Max Planck Institute in Heidelberg), has developed a whole mouse brain chemical preservation and plastic embedding technique. A preliminary overview of the protocol used can be found at http://connectomes.org/ . "14:13
kanzure"This innovative approach begins by transcardially perfusing the mouse with the fast-diffusing fixative acrolein (as opposed to the more traditional fixatives of paraformaldehyde and glutaraldehyde). The protocol then uses simple immersion of the entire mouse brain in solutions including osmium tetroxide over a two day period to achieve fixation of lipids throughout the brain."14:14
kanzure"second team, 21st-Century Medicine led by the cryobiologist Greg Fahy, has developed a method of perfusing entire rabbits with very high concentrations of the cryoprotective agent M22 (a formula that they have developed for commercial use in organ cryopreservation for transplantation). "14:14
kanzure"at the BPF have also made progress in obtaining access to the equipment and facilities needed for evaluating the prize. Last month, I personally visited Halcyon Molecular (a biotech startup company based in San Franscisco) which has graciously offered to let us use their electron microscope facilities to help in prize evaluation. "14:14
kanzurepfft14:14
delinquentme3 5 10 30 6014:20
delinquentmevisionary: im gonna make a simple AI which only knows plant-related terms and im going to sell it on the app store... and name it "words with fronds"14:21
kanzureyour visions don't sound that intense14:21
kanzurehave you tried drugs14:21
delinquentmeLOL14:24
delinquentmebut its plant material14:24
delinquentmeOMGZ #biology will comprehend my mastery14:24
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delinquentmekanzure, happen to know whos the go-to syringe supplier out there/14:28
kanzurenot off han14:32
kanzurehand14:32
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delinquentmeheh. maths is awesome15:58
delinquentmetrue life story... nL are hella frikkin small16:52
delinquentmekanzure, what do people use as nL pumps currently?16:52
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roksprokdelinquentme: http://www.sigmaaldrich.com/labware/labware-products.html?TablePage=10122510420:26
roksproknanoliter pumps20:26
roksprokthat you probably already know about20:26
roksprokonly 2000 dollars20:26
roksprokand backordered20:26
delinquentme:D20:27
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kanzuredelinquentme: they use expensive syringe pumps21:10
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ybitidea: kickstarter for FLOSS development22:29
ybityay/nay?22:29
Steel2FLOSS?22:29
ybitopen source software22:29
Steel2what is the FL part?22:30
Steel2freeware license?22:30
ybitfree/libre22:30
ybitSteel2: think linux22:31
ybitkde22:31
ybitgnome22:31
ybitpython22:31
ybithaskell22:31
ybitetc.22:31
kanzureFLOSS is a pretty standard acronym22:31
kanzureybit: why not use kickstarter?22:32
ybiti don't see any software projects on there22:32
kanzureoh iirc it's against their terms22:32
ybiti dunno if it's allowed22:32
kanzurei guess that's an issue.22:32
ybithuh22:32
kanzurethey don't want you to put your kickstarter project on kickstarter22:33
ybitah22:33
ybitwell here's the problem...22:33
ybitkde e.v. has been in the talks with http://sfconservancy.org/ for a couple of years now about getting a NPO started in the states but SFC got back with them sometime last year to let them know that they just didn't have the capacity22:34
ybithttp://www.youtube.com/watch?v=9q8LTZSvpr8&feature=player_embedded22:34
ybiti'm watching bradley kuhn from there give his presentation ^22:34
kanzurewhat about spi or something22:34
ybitthey fund individual developers22:35
kanzurespi?22:35
ybitwhat is that?22:35
kanzuresoftware in the public interest, inc. (a 501c3)22:35
ybitprobably because nobody had heard of them22:35
kanzurehttp://www.spi-inc.org/22:35
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ybiti'll contact them22:36
kanzureuh really? debian, drupal, yafaray, haskell, libreoffice, jenkins, fluxbox22:36
kanzurestop me if you've heard of any of these :/22:36
kanzureoh and arch linux apparently. neat..22:36
ybitfirst time i've heard of haskell22:36
ybitand wtf is debian22:36
kanzureand postgresql22:36
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kanzureyou know, it's somewhat alarming the number of postgresql installations i've done work on but haven't written direct queries for22:38
kanzurecompared to mysql where the ratio is probably flipped22:38
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ybithttp://pyvideo.org/category/17/pycon-us-2012 :: pycon 2012 vids22:43
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joshcryer<3 yafaray23:02
klafkahi23:07
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--- Log closed Sun Mar 11 00:00:46 2012

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