| --- Log opened Sat Apr 14 00:00:34 2012 | ||
| diginet | urgh, have a protein synthesis machine would be bloody awesome | 00:03 |
|---|---|---|
| diginet | *having | 00:05 |
| kanzure | go get one.. | 00:17 |
| kanzure | http://www.ebay.com/itm/Applied-Biosystems-Peptide-Synthesizer-432A-/230695576437?pt=LH_DefaultDomain_0&hash=item35b6872375#ht_4491wt_1274 | 00:17 |
| kanzure | $1k | 00:17 |
| kanzure | haha this is so ridiculous | 00:18 |
| kanzure | http://www.ebay.com/itm/Applied-Biosystems-Expedite-8909-Nucleic-Acid-Synthesizer-/280777705321?pt=LH_DefaultDomain_0&hash=item415fa7c769#ht_500wt_1289 | 00:18 |
| diginet | I doubt that is capable of synthesizing any peptide long enough for my purposes | 00:18 |
| kanzure | that's almost as tall as i am | 00:18 |
| kanzure | "The Expedite™ 8909 Nucleic Acid Synthesis System is an economical, versatile platform that synthesizes DNA and RNA oligonucleotides as well as peptide nucleic acids" | 00:19 |
| kanzure | economical? bah | 00:19 |
| kanzure | oh man $639 milligen synth on ebay.. | 00:20 |
| kanzure | and for $750+shipping you can get one with a manual? damn | 00:20 |
| diginet | that's a. . .really good deal | 00:21 |
| diginet | like, if I had the money I would totally buy that | 00:21 |
| kanzure | yeah i think i'll get it | 00:21 |
| diginet | seriously? | 00:21 |
| kanzure | sure why not | 00:21 |
| diginet | could you synthesize for me? | 00:21 |
| kanzure | no it probably doesn't work | 00:22 |
| diginet | ohhhhh | 00:22 |
| kanzure | http://www.ebay.com/itm/Millipore-MilliGen-Cyclone-Plus-DNA-Synthesizer-8400-/130530382334?pt=LH_DefaultDomain_0&hash=item1e643791fe#ht_653wt_1274 | 00:22 |
| kanzure | "Millipore MilliGen/Biosearch DNA Synthesizer. Model 8400 Cyclone Plus. Unit comes with Operators Manual, Chemistry Cartridge: H-Phosphate (Version: PV1.07) and Power Cord. Unit powers on to a bright and clear display and responds to commands. We do not have the resources to test further but are giving a 14-day warranty." | 00:22 |
| diginet | well, do you think you could fix it? | 00:22 |
| kanzure | if i buy it i will send it to fenn or nmz787 | 00:22 |
| diginet | ahhhh | 00:22 |
| diginet | how nice of you! | 00:22 |
| diginet | I wonder if there is a way to catalyse peptide bonding besides the way ribosomes do it | 00:23 |
| kanzure | it's in arizona.. shipping's a pain | 00:23 |
| kanzure | well they end in >10 days each so i'll wait on it and see what the others think | 00:25 |
| diginet | ah okay | 00:26 |
| diginet | but anyway | 00:26 |
| diginet | do you think there's any reasonable way to catalyze peptide bonds without ribosomes? | 00:28 |
| yashgaroth | you mean chemical synthesis? we covered that | 00:29 |
| diginet | well yes, but you said that's absurdly slow | 00:29 |
| yashgaroth | it's still the state of the art if you don't like ribosomes | 00:29 |
| diginet | I understand, but I'm saying how could it be improved to at least approach the speed of a ribosome | 00:30 |
| kanzure | yashgaroth: yea or nay on that synthesizer | 00:31 |
| yashgaroth | uhhhhh well I do like biosearch | 00:32 |
| yashgaroth | why don't you guys build a microfluidic oligo synthesizer :V | 00:38 |
| kanzure | working on it | 00:38 |
| kanzure | i'm allowed to have toys too you know.. | 00:39 |
| yashgaroth | heh | 00:39 |
| kanzure | if my toys are pokemon and dna equipment then so be it | 00:39 |
| kanzure | hrm.. $4900 SEM scope | 00:40 |
| kanzure | oh neat. lots of el cheapo syringe pumps. | 00:47 |
| diginet | so why is ribosomal cell free expression slower than cellurar? | 00:52 |
| kanzure | cytoplasm/environment differences | 00:54 |
| kanzure | that's my understanding at least. | 00:54 |
| yashgaroth | who said it was slower | 00:55 |
| diginet | it isn't slower? | 00:55 |
| diginet | I thought it was? | 00:55 |
| yashgaroth | nah | 00:56 |
| diginet | ohhh | 00:56 |
| diginet | hmmm | 00:56 |
| diginet | could cell free be competitive for macroscopic production? | 00:56 |
| yashgaroth | could? maybe. is? no | 00:58 |
| diginet | why is it not as of now? | 00:58 |
| yashgaroth | cellular production is more efficient | 00:58 |
| kanzure | life forms optimized to make things for you. | 00:58 |
| yashgaroth | if something's a shit expressor in cells, it's usually due to problems related to folding anyway, which in vitro doesn't solve | 00:59 |
| yashgaroth | its main use now is dna/rna display panning, or at least that's all I'd use it for | 01:00 |
| diginet | ah, I see | 01:00 |
| diginet | if someone figured out a way to make a machine which could reliably synthesize proteins of arbitrary length (to an extent) that would be. . .wow | 01:01 |
| diginet | just amazing | 01:01 |
| yashgaroth | Jeff McEvolution did that a while ago | 01:01 |
| yashgaroth | try doing silk proteins in vivo first, then start worrying about in vitro if it doesn't work | 01:02 |
| diginet | I know, I'm just saying it would be cool | 01:02 |
| kanzure | try not killing your gfp culture first | 01:02 |
| kanzure | night | 01:03 |
| diginet | well, the good news is that I found out that purifying the proteins is actually really easy | 01:13 |
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| diginet | I think duckweed should prove a very good expression system | 01:13 |
| diginet | I need to do some experiments on optimizing growth though | 01:13 |
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| diginet | http://atp.ncifcrf.gov/proteins-and-proteomics/protein-expression-laboratory/pel-services/high-yield-protein-synthesis-using-cell-free-protein-expression-system | 01:21 |
| diginet | hmmm | 01:21 |
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| Mariu | I have to split, laters | 03:25 |
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| diginet | wow, synthetic life is really interesting | 03:45 |
| diginet | I would be more interested in synthetic life that differs from natural life, like that uses a different nucleic acid or something | 03:45 |
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| diginet | you know what we need? open source retrosynthetic analysis | 05:39 |
| delinquentme | and you use legal to protect open source? I just had a thought that say you being supplying to a manufacturer and they sign an exlcusive agreement for say a year | 05:42 |
| delinquentme | on whatever youre building ... | 05:42 |
| delinquentme | that can act to support dev of your open source hardware | 05:43 |
| delinquentme | http://en.wikipedia.org/wiki/Retrosynthetic_analysis | 05:43 |
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| kanzure | beep boop | 06:24 |
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| delinquentme | meh! gmail is being | 09:01 |
| delinquentme | lame | 09:01 |
| diginet | is 1 g/L a day a reasonable yield to expect of a recombitant protein expression system? | 09:14 |
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| kanzure | looks like qcad has gone commercial | 09:50 |
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| kanzure | and the open source project forked to http://librecad.org/ | 09:50 |
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| kanzure | juri_'s building an open source electron microscope | 10:00 |
| kanzure | he's asking for $250k but i haven't seen his BOM | 10:01 |
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| kanzure | hi _F7_ | 12:38 |
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| delinquentme | surely there are standards established for what volume constitutes a droplet at 1 atmosphere | 13:03 |
| kanzure | a few picoliters | 13:08 |
| kanzure | is a microdroplet. | 13:08 |
| kanzure | i'm pretty sure there's a maximum size due to surface tension vs. gravity considerations | 13:09 |
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| _F7_ | test | 14:21 |
| _F7_ | Okay cool | 14:21 |
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| augur | anyone read society of mind? | 15:13 |
| kanzure | one of marvin's students was in here the other day, does that count :p | 15:22 |
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| ParahSailin | i dont think http://blog.opensourceecology.org/2010/12/open-source-aluminum/ is workable | 16:41 |
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| thylne | [20:34] <noumena> Humans haven't lived in industrial societies for that much of our history | 17:35 |
| thylne | [20:34] <EkurioX> Amazing isn't it... You should listen to Al gore... Smart dude. | 17:35 |
| thylne | [20:35] <mrdata> we reached the singularity in 2004 | 17:35 |
| thylne | >_> | 17:35 |
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| strangewarp | thylne: god, that is painful | 17:42 |
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| delinquentme | kanzure, how do I get solidworks legitimate and cheap? | 18:45 |
| delinquentme | do they sell old versions for less? | 18:45 |
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| kanzure | delinquentme: become a student somewhere | 19:09 |
| kanzure | or join a company that pays for a copy | 19:09 |
| kanzure | :/ | 19:10 |
| delinquentme | commerical license | 19:10 |
| delinquentme | commercial ** | 19:10 |
| kanzure | well you could always pay them directly | 19:10 |
| kanzure | have you considered using alibrecad instead? | 19:10 |
| delinquentme | i think i've got an old license | 19:12 |
| kanzure | also if you draw on a napkin and squint reallly hard it sorta looks like actual work :/ | 19:14 |
| delinquentme | CHECK | 19:15 |
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| thylne | strangewarp the stupid was making my eyes burn :D | 19:45 |
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| kanzure | Adifex: hi | 22:01 |
| Adifex | wazzup | 22:01 |
| Adifex | this place seemed pretty dead last time I logged on, is there anyone here? | 22:02 |
| kanzure | yes we're all just afraid of you | 22:02 |
| kanzure | how do you know us? | 22:02 |
| Adifex | oh I don't. I'm just a lurker here mostly. | 22:09 |
| ybit | Adifex: utdallas, aye? | 22:21 |
| ybit | what are you studying there? | 22:21 |
| Mariu | Adifex, how do you know of us ? | 22:26 |
| katsmeow-afk | http://laughingsquid.com/choc-creator-the-chocolate-3d-printer-is-now-available-to-purchase/ | 22:26 |
| kanzure | katsmeow-afk: i prefer my chocolate to be 6-axis milled | 22:27 |
| Mariu | :D | 22:28 |
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| --- Log closed Sun Apr 15 00:00:36 2012 | ||
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