| --- Log opened Tue May 01 00:00:00 2012 | ||
| --- Day changed Tue May 01 2012 | ||
| @kanzure | and i picked one a bit further back.. theoretically i should have picked the one prior to your first commit | 00:00 |
|---|---|---|
| nmz787 | on checkout does it change all the local files? | 00:00 |
| @kanzure | yes | 00:00 |
| @kanzure | or, rather.. in this case, most of the files are the same | 00:00 |
| nmz787 | so if i cat a certain file, it will change from git checkout master to git checkout ubuntu-fixes? | 00:00 |
| @kanzure | in that situation "git checkout" will complain if it would overwrite uncommitted changes | 00:01 |
| nmz787 | (if said file differed in the branches) | 00:01 |
| @kanzure | jrayhawk: please stop me if i am bullshitting anywhere | 00:01 |
| nmz787 | hmm, no it works | 00:01 |
| nmz787 | checkout master, takes a second maybe... nanoengineer wont start | 00:02 |
| nmz787 | checout ubuntu-fixes, it starts | 00:02 |
| nmz787 | just quick, lol | 00:02 |
| nmz787 | quick enough that i questioned if the files were changning | 00:02 |
| nmz787 | but yeah i guess its basically just a patch command underneath | 00:02 |
| @kanzure | yes each commit is a patch | 00:03 |
| @kanzure | git reassembles the state of the working directory based on deltas calculated from lists of patches | 00:03 |
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| @kanzure | you can see an individual patch too.. | 00:06 |
| @kanzure | git show a74fea | 00:06 |
| nmz787 | lol | 00:06 |
| nmz787 | ok i need to get to bed | 00:06 |
| nmz787 | do we know anyone who can extend this software? | 00:07 |
| nmz787 | is it worth doing that? | 00:07 |
| nmz787 | lol, do we need to model all our reactions using this software? | 00:07 |
| nmz787 | and our channels | 00:07 |
| @kanzure | the original nanorex guys are sorta around but they all have different jobs | 00:07 |
| @kanzure | i don't think this is necessary for our dna synthesis work | 00:08 |
| @kanzure | but it would be interesting | 00:08 |
| nmz787 | ok ttyl | 00:09 |
| @kanzure | seeya | 00:09 |
| nmz787 | oh do you know anything about the company isec partners? | 00:09 |
| nmz787 | and also http://www.isecpartners.com/blog/2010/10/13/isec-acquired-by-ncc-group.html | 00:10 |
| nmz787 | chandni interviewed friday, no word back yet | 00:10 |
| @kanzure | no not off the top of my head | 00:12 |
| nmz787 | seems like before being bought by british company ~30 people | 00:13 |
| nmz787 | not too big, could be nice | 00:13 |
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| strangewarp | http://picturesforsadchildren.com/post/22145925135 - An amusing take on H+ | 03:02 |
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| JayDugger | Good morning, everyone. | 06:11 |
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| @kanzure | beep | 06:37 |
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| @kanzure | nmz787: hi | 08:51 |
| nmz787 | hi | 09:03 |
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| nmz7871 | is trans-sexual considered trans-humanism? | 10:12 |
| katsmeow-afk | are they any different humans than normal un trans humans? | 10:13 |
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| nmz787 | its hard to imagine why someone would want to hide their chromosome expression (i.e. that they actually have XX or XY sex chromosomes)... i mean i guess in the context of the movie gattaca, where its insurance and discrimination, it makes more sense to me | 10:13 |
| katsmeow-afk | i think to be transhuman they must be different than normal humans | 10:14 |
| nmz787 | so i guess its all to do with social discrimination | 10:14 |
| nmz787 | hmm | 10:14 |
| katsmeow-afk | ah, there's more to it than XX and XY in some cases | 10:14 |
| nmz787 | so since homosexuality is prevalent in lower animals and human, is it then normal? | 10:14 |
| nmz787 | you mean like intersex? | 10:14 |
| katsmeow-afk | no, intersex is yet another ball of wax | 10:15 |
| nmz787 | or there are XX chromos, but the hormone levels are screwed up since birth? | 10:15 |
| vrs | nmz787: one could argue they are the original transhumanists | 10:15 |
| katsmeow-afk | there's XXY, XYY, even XXXY | 10:15 |
| nmz787 | hmm | 10:15 |
| nmz787 | so is that natures way of thinning the herd? | 10:15 |
| nmz787 | self-limiting, worker bees, etc? | 10:16 |
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| * katsmeow-afk suggests you read up on it | 10:16 | |
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| nmz787 | really want opinions/conversations, not book reading | 10:18 |
| @kanzure | nmz787: transsexual isn't usually considered transhuman | 10:19 |
| nmz787 | its interesting because i know ppl who are gender-unsure and f2m | 10:19 |
| nmz787 | but also seems like they are, in this society, political justice fighters | 10:20 |
| katsmeow-afk | nmz787 , things NOT binary: physical sex at birth, physical sex after puberty, gene contents, hormone levels, body responce to hormone levels, brain construction related to genes or hormones, etc | 10:20 |
| vrs | I come from the biohacking side and that scene (if you can call it that) definitely has its share of genderqueer etc. people | 10:20 |
| @kanzure | the biohacking side isn't particularly queer :P biocurious was mostly a joke | 10:20 |
| nmz787 | i.e. if gandhi never would have made a big deal about india, they wouldn't have found freedom from british... but also his life was full of turmoil | 10:20 |
| vrs | you need a certain attitude to your body to even consider putting metallic things in there | 10:20 |
| @kanzure | you don't need to be queer to want to inject impractical things under your skin | 10:21 |
| @kanzure | and you don't need genderbending to do it either | 10:21 |
| vrs | right | 10:21 |
| @kanzure | so stfu | 10:21 |
| vrs | I think you missed my point | 10:21 |
| @kanzure | probably | 10:21 |
| katsmeow-afk | the tv show House did a interesting, harsh take on a normal XY person with normal hormones but zero body responce to testosterone | 10:21 |
| nmz787 | hmm | 10:22 |
| vrs | when you're trans/gq/whatever, you have a relationship with your body that cis people don't have | 10:22 |
| nmz787 | i dunno, my body is just a vehicle | 10:22 |
| katsmeow-afk | cis? | 10:22 |
| @kanzure | vrs: and what is that relationship? many people reject their bodies and don't need to be genderbending | 10:22 |
| nmz787 | its got a semen maker | 10:22 |
| nmz787 | whatev, if i woulda been born a female, i would have liked that too | 10:23 |
| vrs | kanzure: I never said you had to be queer to do x, I said being queer makes you more likely to do x | 10:23 |
| nmz787 | i guess i'm interested in body-bending, but the sex part of it isn't that interesting or attention grabbing | 10:23 |
| vrs | cis = not trans | 10:23 |
| katsmeow-afk | nmz787, for those females that egaculate, is that "a semen maker" ? | 10:23 |
| strangewarp | Ideally, there would be a technology that allows people to absolutely choose what sort of body they would have. In lieu of that, one must choose between pursuing current techniques and waiting for better techniques. (If you don't care about what sort of body you have, that's fine too, but don't exclude the people who do care.) | 10:23 |
| nmz787 | i.e. girl or boy parts, i would think about electroporating myostatin inhibitors | 10:24 |
| vrs | when you're willing to undergo hormone treatment and possibly SRS, a piece of metal in your body is just another operation | 10:24 |
| @kanzure | strangewarp: changing your sex organs doesn't particularly impact your ability to do better transhuman technologies | 10:24 |
| katsmeow-afk | strangewarp, interesting take on it :-) | 10:24 |
| vrs | and the 'normal' people I've talked to about implants etc were quite put off by it | 10:24 |
| vrs | too much yuck for them | 10:25 |
| @kanzure | strangewarp: i guess you might say, "well, if i don't turn my dick inside out, i would be very unable to be effective at my work" | 10:25 |
| @kanzure | *claim | 10:25 |
| katsmeow-afk | kanzure, there's other methods of srs | 10:25 |
| @kanzure | oh thank god | 10:25 |
| nmz787 | i just really wonder if the hormonal stuff is reliable or not | 10:25 |
| strangewarp | kanzure: I think you think I'm making a claim I'm not making? :s | 10:25 |
| vrs | nmz787: srs is only part of it | 10:25 |
| nmz787 | i don't even think having birth control pills since the 1950s is long enough to just give to teenagers that dont want to get acne | 10:26 |
| vrs | mones alone don't reverse your dick | 10:26 |
| @kanzure | so anyway.. that's why i argue that transsexual srs is not a transhuman technology | 10:26 |
| nmz787 | srs? | 10:26 |
| @kanzure | sex reassignment surgeries | 10:26 |
| katsmeow-afk | it can't be transhuman, because you areleft with 100% plain simple human biology after srs | 10:26 |
| nmz787 | so nootropics/adderall isn't transhuman? | 10:26 |
| nmz787 | its not programmed by the DNA | 10:27 |
| vrs | katsmeow-afk: except that you need to take hormones | 10:27 |
| nmz787 | in srs, hormones, or adderal | 10:27 |
| @kanzure | ah wait i didn't say DNA only did i? | 10:27 |
| nmz787 | kanzure: no | 10:27 |
| katsmeow-afk | vrs, i disagree, not in all cases | 10:27 |
| katsmeow-afk | besides, the hormones are replacement, not transhumanistic | 10:27 |
| @kanzure | nmz787: i would argue that you can use (and measure) nootropics to impact the human brain | 10:28 |
| vrs | nmz787: http://sapiensanonym.blogspot.de/ | 10:28 |
| @kanzure | lepht is a crack pot | 10:28 |
| vrs | anecdotal evidence :) | 10:28 |
| nmz787 | so is a transhuman different from their birth composition, or different from the avg/reference human? | 10:28 |
| -!- vrs was kicked from ##hplusroadmap by kanzure [vrs] | 10:28 | |
| katsmeow-afk | wait, there's NO posting of urls in here anymore?! | 10:28 |
| nmz787 | kanzure: i'm not saying nootropics dont mess with brain | 10:28 |
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| @kanzure | katsmeow-afk: heh not really | 10:28 |
| vrs | hm? | 10:28 |
| @kanzure | vrs: i still love you | 10:28 |
| @kanzure | but seriously, lepht isn't a reasonable person | 10:29 |
| katsmeow-afk | then next time you post an url, please kick yourself | 10:29 |
| nmz787 | why did vrs get kicked? | 10:29 |
| vrs | kanzure: never said it was | 10:29 |
| nmz787 | i post URLs | 10:29 |
| @kanzure | i am just being playful. i didn't ban vrs and i still love vrs. | 10:29 |
| nmz787 | ok | 10:29 |
| katsmeow-afk | a kick is still disruptive and impolite | 10:29 |
| @kanzure | lepht was the one who started conflating psychotic breakdowns with transhumanism and biohacking | 10:29 |
| nmz787 | so kanzure are nootropics transhumanist, or just temporal modification? | 10:29 |
| @kanzure | nmz787: i think temporary modifications could be considered to be human enhancing, yes.. | 10:30 |
| nmz787 | because adderal is seen in academics to give a competitive edge over other students | 10:30 |
| @kanzure | katsmeow-afk: yes well so is linking to lepht | 10:30 |
| vrs | kanzure: deal with it, every scene has its broken people | 10:30 |
| nmz787 | so in that case srs or sex hormones is transhumanist too | 10:30 |
| vrs | and leoht is interesting | 10:30 |
| nmz787 | all the peeps on birth control | 10:30 |
| @kanzure | vrs: lepht isn't part of this scene really :P | 10:30 |
| vrs | lepht* | 10:30 |
| @kanzure | lepht has never actually communicated with the majority of the biohacking community | 10:30 |
| @kanzure | mostly because we'd rip her apart | 10:30 |
| vrs | also if all you ever read about it was that shitty wired article | 10:31 |
| katsmeow-afk | i never heard of her | 10:31 |
| @kanzure | no not just that wired article | 10:31 |
| @kanzure | but also the screaming vids | 10:31 |
| @kanzure | and the arm/tendon shit | 10:31 |
| @kanzure | anyway! | 10:31 |
| @kanzure | nmz787: there's people who would say yes and some who would say no to you on that | 10:32 |
| vrs | elaborate, I watched one of the implant videos, but it didn't have sound | 10:32 |
| strangewarp | I think there is a distinct difference between current SRS, where flesh is moved around with simple surgery, and ideal SRS, which would result in full transformation with no compromise. But trying to figure out whether the former is transhumanist or not may not be relevant. | 10:32 |
| @kanzure | nmz787: they would argue that our nootropics currently suck, for instance | 10:32 |
| @kanzure | there are other ways to define transhumanist though.. | 10:33 |
| @kanzure | like "minimal viable nootropic" | 10:33 |
| * katsmeow-afk goes away to do things irl :-( | 10:33 | |
| @kanzure | which would be a nootropic that makes its user smart enough to make a better nootropic | 10:33 |
| vrs | kanzure: I think you are being an elitist, but ok | 10:33 |
| nmz787 | but also if we're saying nootropics (drugs) could be transhumanist, then majority of ppl are transhumanist in a little way | 10:33 |
| nmz787 | anyone taking any pill | 10:33 |
| @kanzure | vrs: i don't have a backup of the other videos | 10:33 |
| nmz787 | because pills are so new to human race | 10:33 |
| @kanzure | vrs: i think you should not take her advice and think for yourself instead | 10:34 |
| nmz787 | transhumans dont have to be smarter, right??? | 10:34 |
| vrs | kanzure: obviously | 10:34 |
| nmz787 | they could be total dumbasses that survive on rock slime | 10:34 |
| @kanzure | vrs: that's /not/ obvious to lepht's followers | 10:34 |
| strangewarp | If you're going to widen the definition that much, you might as well include clothes and tools | 10:34 |
| vrs | also "its", not "her" | 10:34 |
| @kanzure | vrs: yawn.. i don't care | 10:34 |
| vrs | kanzure: I think you've only met the idiot ones | 10:34 |
| @kanzure | vrs: on that point i'm elitist ;) | 10:34 |
| nmz787 | well kanzure the pronouns are a non-binary thing like someone mentioned earlier | 10:34 |
| @kanzure | yes but language is not specific anyway | 10:35 |
| @kanzure | i am not going to fully qualify all of the things that i say | 10:35 |
| vrs | there are not that many things that make sense as implants, sadly | 10:35 |
| vrs | at least with the current state of technology | 10:35 |
| @kanzure | a magnet is interesting but i don't think it's particularly relevant | 10:35 |
| @kanzure | and i don't see a reason to be opposed to aneasthetics | 10:35 |
| @kanzure | nmz787: so that's the thing, | 10:36 |
| vrs | kanzure: because lepht has a certain history with anaesthetics | 10:36 |
| @kanzure | nmz787: transhumanism really just means "human enhancement" | 10:36 |
| @kanzure | nmz787: there are many technologies that improve an individual's personal condition | 10:36 |
| vrs | half of the blog entries are about problems with tramadol | 10:36 |
| vrs | (or they used to) | 10:37 |
| @kanzure | nmz787: some of these are more empowering than others.. with the right levers and pullys you can move much more than your body mass | 10:37 |
| @kanzure | *pulleys | 10:37 |
| @kanzure | but with a computer you can resequence your dna | 10:37 |
| nmz787 | i guess with the ppl i know who are going f2m... i wonder if its some deep-rooted subconscious "the grass is greener on the other side" | 10:38 |
| nmz787 | I know it could just be preference | 10:38 |
| @kanzure | nmz787: most people have very different goals than you or i | 10:38 |
| @kanzure | they just want a job and a family and a house and to not feel like crap in the morning, or something | 10:38 |
| nmz787 | yeah | 10:39 |
| nmz787 | i mean i def felt like a small kid in high school, i wasn't a jock or anything, didn't know how to work out, avoided physical confrontation because i felt weak | 10:39 |
| nmz787 | tried working out, but didn't have a teacher, so didn't make much progress | 10:39 |
| nmz787 | i guess that stressed me out, but i also kinda didnt care since i was in my own book/internet world | 10:40 |
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| nmz787 | also i think my male hormone stream is pretty good, always been keen on the womens | 10:40 |
| @kanzure | pfft there's always someone with more hormones (brain tumors hooray) | 10:41 |
| nmz787 | so i can try to understand that if my hormones were fucked, it woulda created a lot more confusion | 10:41 |
| nmz787 | and maybe i wouldn't have been studying bookish shit so much, with more stress | 10:41 |
| vrs | >i wonder if its some deep-rooted subconscious "the grass is greener on the other side" | 10:43 |
| @kanzure | no people who are trans explain it as something different i think | 10:43 |
| vrs | science isn't yet finished on that one, but the most popular theory is "the brain's internal representation of genitals and body doesn't fit the real thing" | 10:44 |
| vrs | and it's proven easier to mould the body instead of the brain | 10:45 |
| nmz787 | ok so but the reality doesnt change | 10:45 |
| nmz787 | wetware is easier to change, no? | 10:46 |
| nmz787 | take some psychedelics or something | 10:46 |
| vrs | not at the deeper levels, no | 10:46 |
| vrs | psychedelics don't help against homosexuality either :) | 10:46 |
| nmz787 | not against | 10:46 |
| nmz787 | but refitting the wetware | 10:46 |
| nmz787 | getting ahold of, these masses are my cells | 10:46 |
| vrs | with wetware you mean the brain? | 10:46 |
| @kanzure | sometimes it's concomitant with pervasive development disorder | 10:47 |
| nmz787 | these masses called balls, are part of the same unit that holds my conscious | 10:47 |
| nmz787 | i can understand, ok, balls aren't my ideal mass to be down there | 10:47 |
| nmz787 | but to be so frustrated | 10:47 |
| @kanzure | they aren't thinking like that | 10:47 |
| nmz787 | seems pointless or a waste of time | 10:47 |
| strangewarp | It sounds like you're rationalizing | 10:48 |
| nmz787 | unless they are frustrated with how society discriminates them | 10:48 |
| @kanzure | their value system or internal psychology is such that gender is on the top of their mind | 10:48 |
| nmz787 | i.e. soceity sees balls, but the person wants to be seen with breasts | 10:48 |
| @kanzure | in your case nmz787 it sounds like you wouldn't be particularly heartbroken if you were genderless | 10:48 |
| yashgaroth | well normally society won't be seeing your balls | 10:48 |
| @kanzure | yashgaroth: then you're doing it wrong | 10:48 |
| nmz787 | strangewarp: yes i try to be rational | 10:48 |
| nmz787 | yashgaroth: sure they do, deep voice, facial hair | 10:48 |
| nmz787 | all go to balls IMO | 10:49 |
| @kanzure | that's your hormones showing | 10:49 |
| strangewarp | I would prefer to be neutrois and without a sex drive, and I plan to make myself as such once I can | 10:49 |
| yashgaroth | sorry, I was being facetious :P | 10:49 |
| @kanzure | females can take hormone injections to get a deep voice and more facial hair | 10:49 |
| nmz787 | kanzure: right, my friend who just started that says she hates her/his breasts.. and she wants the social cues from ppl thinking shes a man | 10:49 |
| @kanzure | nmz787: do you know about dismorphic syndromes? where the brain (either through some psychological method or physical reaction) rejects a limb or part of the body | 10:50 |
| nmz787 | sure, V.S. ramachandran, bulemia, anorexia?? | 10:50 |
| @kanzure | nmz787: that sounds more like a reason to be a cross dresser.. not trans | 10:50 |
| nmz787 | (my mom is/was bulemic) | 10:50 |
| @kanzure | no | 10:50 |
| @kanzure | like people who cut off their legs because "THIS SHOULDN'T BE THERE" | 10:50 |
| nmz787 | kanzure: fact is she/he is taking T | 10:50 |
| nmz787 | oh | 10:51 |
| nmz787 | that seems pretty damn far | 10:51 |
| vrs | transpeople cut their dicks off too in extreme cases | 10:51 |
| nmz787 | i've heard of ppl getting shot in neck in war, and 'feeling' the bullet move around, and cutting their face/head apart with scalpel to try and extract the fragments.... | 10:51 |
| vrs | or, try to | 10:51 |
| strangewarp | kanzure: There are also people who feel like they should have /very/ different or extra parts, like a subset of the people who are into furry | 10:52 |
| nmz787 | but that is grounded in reality to begin with | 10:52 |
| @kanzure | nmz787: there are many things that can go wrong in the human brain long before you get to a stage where something resembling rational thought can happen | 10:53 |
| @kanzure | and by wrong i mean abnormal really | 10:53 |
| strangewarp | The easiest way to solve body dysmorphic thoughts is to let the person modify their body, but instead it's treated as something that must be repressed.. | 10:55 |
| @kanzure | oh right, "body dysmorphic" is what i was thinking of | 10:56 |
| @kanzure | strangewarp: there are many advantages to keeping your legs | 10:56 |
| @kanzure | right now we don't have anything better. wheelchairs aren't necessarily healthy. exoskeletons tend to suck. | 10:57 |
| strangewarp | One can make any argument for the objective utility of a particular boily configuration, but if the person's mind wants something else, then they're either going to have to change their mind or their body. | 10:57 |
| @kanzure | and the prosthetics are neat but once you have one prosthetic you use up all your pectoral muscles with electrodes or something lame like that | 10:57 |
| nmz787 | bbl | 10:57 |
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| strangewarp | boily=bodily* | 10:57 |
| strangewarp | And yeah, this is why I'm waiting until we have better methods of body modification before I go neutrois. Current methods are expensive, painful, illegal, and have moderately okay results at best. | 10:58 |
| @kanzure | "illegal" shouldn't be a concern to you | 10:59 |
| @kanzure | do you know about that secret island body mod convention? | 10:59 |
| yashgaroth | chemical castration? though you've doubtless looked into that more than I have | 11:00 |
| vrs | what strangewarp says | 11:00 |
| strangewarp | kanzure: Nope, but I'm not really into conventional bodmods. Most of what I want is not presently possible. (I arrived at transhumanism after drifting through furry and becoming unsatisfied with the resigned sense of never-being-able-to-get-the-body-you-want, for some perspective here) | 11:01 |
| strangewarp | yashgaroth: Chemical castration has nasty side-effects and leaves the genitals intact, both of which are negatives to me. | 11:02 |
| @kanzure | strangewarp: i want to reiterate how much i appreciate that you have never linked to a rule 34 image in here | 11:02 |
| strangewarp | I try to have decorum ;) | 11:02 |
| vrs | strangewarp: hm, do you want to keep the sensitive parts or be completely sexless? | 11:02 |
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| strangewarp | vrs: Ideally, I'd remove my sex organs and sex drive completely. | 11:03 |
| vrs | as in, no dick, no clit, nothing but a pisshole | 11:03 |
| vrs | ah | 11:03 |
| yashgaroth | I do wonder how much of an effect physical castration would have on an adult, since it's usually done in childhood | 11:03 |
| yashgaroth | (in terms of side effects, not the obvious ones) | 11:04 |
| @kanzure | surely the soviets have experimented on this | 11:04 |
| @kanzure | quick! to the soviet learnatorium | 11:04 |
| vrs | that should be easy enough, most of the difficulty of SRS comes from trying to preserve the nerves | 11:04 |
| strangewarp | yashgaroth: Stops the progression of baldness, drastically reduces sex drive, causes some individuals to gain 15 pounds or so | 11:04 |
| vrs | yashgaroth: http://news.bmezine.com/wp-content/uploads/2008/09/pubring/people/A10101/gelding.html | 11:04 |
| vrs | he kept his dick though | 11:05 |
| yashgaroth | tbh that'd just make it easier to piss | 11:05 |
| nmz787_ | The dick is def just a hose that makes pissibg less messy | 11:07 |
| yashgaroth | I'm planning to keep my balls, mainly since spermatogonial stem cells are the best quality for reprogramming | 11:07 |
| vrs | balls produce a good part of your testosterone though | 11:07 |
| nmz787_ | Thats a plus of semen, cheap stem cells, though only haploid | 11:08 |
| nmz787_ | Or ejaculate rather | 11:08 |
| yashgaroth | I mean the diploid progenitor cells | 11:09 |
| nmz787_ | Hard to get then out though? | 11:09 |
| nmz787_ | You have to surgery them out?? | 11:09 |
| yashgaroth | if you're lucky, just a needle and a general anesthetic | 11:10 |
| strangewarp | ow | 11:10 |
| nmz787_ | Interesting | 11:10 |
| yashgaroth | pretty much all other cells in the body undergo a lot of dna methylation, which degrades their usefulness in being reprogrammed to other cell types | 11:10 |
| yashgaroth | since methylation causes a lot of mutations, and they're naturally methylating the regions that they don't use, that other cell types would | 11:11 |
| yashgaroth | but yeah if you think muscle electroporation would be painful, that's a whole other level of ouch | 11:12 |
| nmz787_ | Cant you inject demethylase? | 11:12 |
| yashgaroth | sure, but a lot of the mutations are already there | 11:12 |
| yashgaroth | reprogrammed cells get demethylated anyway, but the mutations remain | 11:13 |
| nmz787_ | With nuclear signal on it with viral promoter | 11:13 |
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| yashgaroth | reprogramming cells leads to genome replication, with fewer and fewer of the methylation sites being copied onto the daughter genome | 11:13 |
| nmz787_ | Mutations in accesibility bc of methyls, or dna gets "damaged" | 11:13 |
| yashgaroth | methylated Cs will spontaneously mutate | 11:14 |
| yashgaroth | to Ts | 11:14 |
| nmz787_ | But remain methylated or that can chabge too? | 11:14 |
| yashgaroth | when the genome is copied in replication, the new genome is unmethylated | 11:15 |
| yashgaroth | usually a methyltransferase comes in to make all the sites methylated that should be, or were on the parent genome | 11:15 |
| nmz787_ | Wha? | 11:15 |
| nmz787_ | Ya | 11:15 |
| nmz787_ | So the new Ts dont have parents... so no methyls | 11:16 |
| yashgaroth | err no that's a separate issue | 11:16 |
| yashgaroth | sometimes a methylated C becomes a T, which is a problem for reprogramming | 11:17 |
| yashgaroth | but also for inducing reprogramming, you want to block methylation in general | 11:17 |
| nmz787_ | Because loss of functionality | 11:18 |
| nmz787_ | Or onco jeans turn on | 11:18 |
| nmz787_ | ?? | 11:18 |
| yashgaroth | well you want the oncogenes on during reprogramming | 11:18 |
| nmz787_ | I mean permanently due 2 mutation | 11:19 |
| yashgaroth | that could happen as well, sure | 11:19 |
| jmil | kanzure: http://www.nature.com/nrendo/journal/vaop/ncurrent/full/nrendo.2012.49.html | 11:19 |
| @kanzure | is that it? | 11:19 |
| @kanzure | oh :( that's not your paper. | 11:19 |
| @kanzure | "Muscles, exercise and obesity: skeletal muscle as a secretory organ" | 11:19 |
| nmz787_ | Android text to speech I'm using android text to speech now | 11:19 |
| yashgaroth | oh is that the irisin paper | 11:19 |
| nmz787_ | I mean speech to text | 11:20 |
| yashgaroth | oh it's not, good | 11:20 |
| nmz787_ | Why is that author bad? | 11:21 |
| yashgaroth | who said he was? | 11:22 |
| nmz787_ | U seemed to imply | 11:22 |
| nmz787_ | By saying oh it's not, good | 11:22 |
| yashgaroth | no no, just that irisin was the new big myokine, but that paper was a while ago | 11:22 |
| nmz787_ | Oo irisin is not an author, lol | 11:23 |
| yashgaroth | figure 2's nice, but why does figure 1 have a yinyang | 11:24 |
| yashgaroth | oh they're just being cute | 11:25 |
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| yashgaroth | hey jmil I don't suppose you have the fulltext of that paper maybe? | 11:29 |
| nmz787_ | I can prob get it wheb i get bac onto laptop | 11:29 |
| yashgaroth | awesome | 11:29 |
| nmz787_ | Bio is soooo complex when you look at it, so much to think about from an engineering standpoint | 11:30 |
| yashgaroth | engineering in the classical is nigh impossible at this point, which is why I prefer the hacking metaphor so much | 11:31 |
| nmz787_ | Quickly gets overwhelming unless you really specialize, or only get a surface level broad view | 11:31 |
| nmz787_ | For me at least | 11:31 |
| yashgaroth | hey at least it's fun | 11:33 |
| nmz787_ | I think places with big money and lots of rote knowledge infused workers can prob do OK with classical engi tech | 11:33 |
| nmz787_ | But i guess this is the age where big pharma approach might collapse | 11:34 |
| yashgaroth | oh no I'll be working for big pharma probably :/ | 11:35 |
| nmz787_ | Evolution is experimental, hackish... some works some fails horribly, some slips past bein OK to be great later, ot fail even more horribly later | 11:35 |
| yashgaroth | evolution is a billion years of kludges | 11:36 |
| yashgaroth | the source code is commentless and in a mostly-unknown language, and the hardware is even more of a black box | 11:38 |
| thyla | I agree with this discussion. | 11:38 |
| nmz787_ | Haha ya | 11:42 |
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| @kanzure | hello fortoke-recaptch | 11:58 |
| fortoke-recaptch | recaptcha is a communications interface for terrorists, goto something that can refresh recaptchas, after 5 times it will tell you "secrets" the federals know about you | 11:59 |
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| @kanzure | what the hell is going on | 12:00 |
| @kanzure | this is not the kind of crazy i wanted | 12:01 |
| nmz787_ | Wtf | 12:03 |
| strangewarp | wow. | 12:06 |
| strangewarp | THE FEDS, MAN | 12:06 |
| strangewarp | I have some sympathy for crazy people, since I used to be one and then got better | 12:09 |
| jrayhawk | what precipitated the betterness? | 12:09 |
| @kanzure | jrayhawk: animal love | 12:10 |
| @kanzure | ... i'll show myself out. | 12:10 |
| strangewarp | At the age of 16 I was convinced I could teach everyone in the world how to transform themselves through willpower. The belief ended up colliding with reality, and reality won. ;) | 12:10 |
| @kanzure | what is willpower? | 12:11 |
| strangewarp | Hell if I know, I wasn't the most rational person back then | 12:11 |
| @kanzure | ok. thought maybe you knew something everyone else doesn't. | 12:11 |
| strangewarp | hahaha, that was what I thought at the time, but it was a false belief :P | 12:12 |
| nmz787_ | Nah most ppl dont know shit | 12:13 |
| @kanzure | no i mean if you know how to physically manufacture willpower, that would be worth knowing about | 12:13 |
| strangewarp | Oh, indeed | 12:14 |
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| strangewarp | The closest I've come to /literally/ manufacturing willpower is the effect citicoline and alpha-GPC have on me. With any luck I'll be getting some more in a couple weeks. | 12:15 |
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| strangewarp | And that's not so much "manufacturing" as it is "consuming".. :p | 12:15 |
| @kanzure | err have you considered that maybe willpower is the wrong concept | 12:16 |
| strangewarp | Oh, sorry, back then I was using the word "willpower" in a mystical, mind-over-matter sense, whereas now I use it in the "get motivated to work on stuff" sense | 12:17 |
| @kanzure | some anonymization stuff https://bwprivacy.to/ | 12:42 |
| @kanzure | http://highlyscalable.wordpress.com/2012/05/01/probabilistic-structures-web-analytics-data-mining/ | 12:45 |
| fenn | diy aerogels http://www.aerogel.org/?cat=10 | 13:02 |
| fenn | also, strangewarp, a cheap(er) source of alpha-gpc http://www.beyond-a-century.com/AlphaSize-50WSP--Alpha-GPC-Choline-Powder-50-Grams_p_666.html | 13:02 |
| @kanzure | fennnnn | 13:02 |
| fenn | gack | 13:02 |
| @kanzure | i got nanoengineer working. | 13:02 |
| @kanzure | ... sort of. | 13:02 |
| fenn | great, now all we need is a nano assembler | 13:03 |
| strangewarp | fenn: oooh, thanks! | 13:03 |
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| fenn | interesting, i got fed up and looked up the bash completion thing at about the same time | 13:31 |
| @kanzure | yesterday? | 13:31 |
| fenn | the answer is bash_completion_lib | 13:31 |
| @kanzure | well it turns out i had no swap space | 13:32 |
| @kanzure | after adding a swapfile to my system i haven't had a 5sec bash_completion issue | 13:32 |
| fenn | drwxrwxr-x 20 fenn fenn 4096 2012-04-26 02:28 /home/fenn/local/bash_completion_lib-1.3.1/ | 13:34 |
| fenn | drwxrwxr-x 20 fenn fenn 4096 2012-04-26 02:28 /home/fenn/local/bash_completion_lib-1.3.1/ | 13:34 |
| fenn | drwxrwxr-x 20 fenn fenn 4096 2012-04-26 02:28 /home/fenn/local/bash_completion_lib-1.3.1/ | 13:34 |
| @kanzure | stop reading my brains | 13:42 |
| @kanzure | cute apache footer: "a monkey hitting keys at random on a typewriter keyboard for an infinite amount of time on this Server at brlcad.org Port 80" | 13:50 |
| yashgaroth | oh god some dumbass on biohack.me wants to genemod himself with telomerase in an HIV vector | 13:55 |
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| yashgaroth | so many things wrong there | 13:55 |
| @kanzure | yashgaroth: list them list them | 13:56 |
| @kanzure | also.. did he have a reason for telomerase? | 13:56 |
| yashgaroth | because he'll be immortal lolz | 13:56 |
| @kanzure | uh | 13:56 |
| yashgaroth | ok...1 lentiviruses only infect immune cells | 13:57 |
| yashgaroth | 2 the vector includes a GFP gene which I doubt he was planning to remove, which will cause an immune response anyway | 13:57 |
| yashgaroth | 3 telomerase by itself won't do anything but make him more susceptible to leukemia | 13:57 |
| @kanzure | does he have a particular variant of tolemerase? i don't understand | 13:58 |
| yashgaroth | I think it's the standard wild-type gene | 13:58 |
| @kanzure | so.. uh. | 13:58 |
| nmz787 | overexpression of telomerase would lead to leukemia why? | 13:59 |
| nmz787 | if he cloned his own gene, should be ok, right? | 13:59 |
| nmz787 | or is it that it would cause all cells to live longer | 13:59 |
| @kanzure | telomerase by itself wont cause cells to live longer | 13:59 |
| nmz787 | and you need to cycle immune cells? | 13:59 |
| nmz787 | ok so why would it cause leukemia? | 13:59 |
| nmz787 | gooogle shows association, but not cause | 14:02 |
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| yashgaroth | sorry, dipshit roommate knocked out the router | 14:06 |
| yashgaroth | ok so adding tert to immune cells will predispose them to leukemia | 14:07 |
| yashgaroth | since it's an oncogene | 14:07 |
| yashgaroth | and a strong one at that | 14:07 |
| yashgaroth | he's not cloning his own gene, but it shouldn't matter since it's p. much the same gene for everyone | 14:07 |
| yashgaroth | normally the body uses telomeres to regulate cell death, since a cell with short telomeres is likely to have a lot of mutations | 14:09 |
| yashgaroth | hayflick limit etc. | 14:09 |
| yashgaroth | I won't get into the obsession that amateur biologists seem to have with telomerase in general, but by itself it won't do much to help lifespan | 14:10 |
| nmz787 | i think ideally the body needs a renewal period added | 14:11 |
| nmz787 | where a massive patch is done against one very slow growing cell/organ | 14:11 |
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| nmz787 | unless that is what sleep could be used for | 14:12 |
| nmz787 | but it doesn't seem to be working | 14:12 |
| yashgaroth | well, the various strategies are still being worked out | 14:12 |
| yashgaroth | but what this guy's doing is...well, it probably won't do anything anyway, but still | 14:13 |
| nmz787 | well, as long as he doesn't hurt anyone else... its really just his prob | 14:13 |
| yashgaroth | the GFP coexpression should hopefully trigger a response that kills all the infected cells | 14:13 |
| nmz787 | darwin award type thing | 14:13 |
| fenn | i suggest anyone wishing to live forever start with eating large amounts of green leafy vegetables | 14:13 |
| yashgaroth | I'm just saying he's dumb and making everyone else look bad, and knowing biohack they'll be all "let's do this!" and argh | 14:14 |
| fenn | from there you can move to more powerful technologies like fish oil and dark chocolate | 14:14 |
| nmz787 | fenn, thats monstrous!!! | 14:14 |
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| nmz787 | fenn: thats all homeopathy | 14:15 |
| nmz787 | :D | 14:15 |
| nmz787 | kidding! | 14:15 |
| yashgaroth | I ingest homeopathic levels of leafy greens | 14:15 |
| fenn | i read that 80% of americans are iodine, potassium, and magnesium deficient | 14:15 |
| fenn | i'm sort of disappointed by life extension foundation | 14:15 |
| @kanzure | at least SENS had researchers | 14:16 |
| @kanzure | OH WAIT | 14:16 |
| fenn | on the one hand they make good publicity for scientific studies relating to longevity | 14:16 |
| @kanzure | ParahSailin: didn't they fire you guys? | 14:16 |
| fenn | on the other they charge way too much for drwxrwxr-x 20 fenn fenn 4096 2012-04-26 02:28 /home/fenn/local/bash_completion_lib-1.3.1/ | 14:16 |
| fenn | god dammit | 14:16 |
| fenn | for vitamins | 14:17 |
| @kanzure | LEF has to make back the $15,000 they spend annually on unprofitable webads | 14:17 |
| fenn | bash completion lib only $19.99 | 14:17 |
| fenn | anyway, vitamin K pills from LEF are $18 for the equivalent of a large bag of spinach | 14:17 |
| @kanzure | how many bags of spinach would you need to buy to get any amount of potassium | 14:18 |
| fenn | it's not all that far out of proportion but it gives people the idea that you must be crazy to buy vitamins | 14:18 |
| fenn | spinach isnt the best source of potassium really | 14:18 |
| @kanzure | yes | 14:18 |
| fenn | http://nutritiondata.self.com/facts/vegetables-and-vegetable-products/2626/2 | 14:19 |
| @kanzure | i was just making fun of you for using "bag of spinach" as a unit of comparison | 14:19 |
| @kanzure | most people buy heads of lettuce or something.. i don't know | 14:19 |
| @kanzure | lettuce isn't potassium rich either, so i don't see where you were going | 14:19 |
| fenn | lettuce sucks | 14:20 |
| fenn | apparently kale will cure multiple sclerosis if you eat enough | 14:21 |
| nmz787 | how did JCVI get the chromosome into the 'empty' cell ? | 14:22 |
| yashgaroth | microinjection probably | 14:22 |
| nmz787 | did they bring it in in pieces in bulk electroporation, with gibson assembly enzymes too? | 14:22 |
| nmz787 | wouldn't the chromosome be liable to shear in-vitro? | 14:23 |
| yashgaroth | dunno, lemme find the paper | 14:23 |
| fenn | yeast does some magic recombination | 14:24 |
| nmz787 | fenn: i had the same question about iodine and fluorine (contam) 4 years ago | 14:24 |
| nmz787 | i asked a prof and he said he was a long time endocrinologist | 14:24 |
| nmz787 | and there just aren't any statistics to prove that we're iodine deficient | 14:24 |
| fenn | lack of evidence isn't evidence of lack | 14:25 |
| * fenn looks | 14:25 | |
| nmz787 | hmm, well that was 4 yrs ago | 14:26 |
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| nmz787 | from wiki https://www.ncbi.nlm.nih.gov/pubmed/21640375 | 14:26 |
| fenn | "breast milk from 47% of nursing mothers did not contain sufficient iodine to meet their infants' nutritional needs" and "It is very difficult to measure iodine deficiency in individuals" | 14:27 |
| fenn | why would it be hard to measure? a mass spec reading away | 14:29 |
| nmz787 | is there a way to just upregulate retention of iodine? | 14:29 |
| fenn | nah it's so easy to just add iodine to your diet | 14:29 |
| fenn | seaweed, basically | 14:29 |
| nmz787 | meh | 14:30 |
| fenn | or iodized salt | 14:30 |
| nmz787 | i got sick of dulse | 14:30 |
| nmz787 | i like that fresh seaweed they serve at japanese restaurants | 14:30 |
| jrayhawk | Or pretty much any seafood | 14:30 |
| nmz787 | with sesame oil | 14:30 |
| jrayhawk | which you should eat for plenty of other reasons | 14:30 |
| nmz787 | no clean rivers around me | 14:30 |
| fenn | yes, it turns out we need EPA and DHA | 14:30 |
| nmz787 | and i wont buy fish that came from greenland, was processed in china, and is now in upstate NY | 14:31 |
| fenn | i eat canned sardines and mackerel | 14:31 |
| fenn | better than nothing | 14:31 |
| fenn | actually, it's the best fish to eat from an environmental perspective | 14:31 |
| nmz787 | "best" in today's world | 14:31 |
| jrayhawk | I eat wild-caught shrimp 'cause I'm almost-coastal and get to lord it over the rest of you chumps. | 14:31 |
| nmz787 | not "best" in "best" world | 14:31 |
| fenn | ideally we'd all run on sunlight | 14:32 |
| nmz787 | hah, where are u? | 14:32 |
| jrayhawk | Portland OR | 14:33 |
| nmz787 | nice | 14:33 |
| nmz787 | close to a lotta pretty good water | 14:33 |
| strangewarp | Portland.. lucky | 14:33 |
| strangewarp | I hear they've got a killer music scene | 14:34 |
| strangewarp | Or at least, tons of extremely weird bands | 14:34 |
| jrayhawk | Extremely weird everything. | 14:34 |
| @kanzure | why wouldn't you point out "oh that's where linus lives" | 14:34 |
| @kanzure | instead you point out music? | 14:34 |
| strangewarp | I am a music nerd I guess :x | 14:36 |
| jrayhawk | http://www.google.com/search?q=darth+vader+bagpipe&tbm=vid all that matters is that we have this guy | 14:36 |
| strangewarp | .. the URL made me laugh | 14:36 |
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| fenn | portland, home of the stash tea company | 14:37 |
| strangewarp | Okay, I have more respect for Portland now, having seen that guy | 14:37 |
| fenn | oh, i was thinking tazo tea | 14:37 |
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| fenn | nmz787: "polyethylene glycol-mediated transformation of the circular chromosome to the DNA-free cells followed by selection" | 14:41 |
| fenn | PEG exposure often spontaneously creates cells with extra chromosomes | 14:41 |
| fenn | weird stuff | 14:42 |
| yashgaroth | err where did you see that part | 14:43 |
| fenn | http://en.wikipedia.org/wiki/Mycoplasma_laboratorium | 14:44 |
| yashgaroth | no the part about extra chromosomes | 14:44 |
| fenn | we did that in my cell culture lab | 14:44 |
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| fenn | erm. the tissue culture lab i took in college | 14:44 |
| yashgaroth | it can make cells fuse, but they won't spontaneously develop chromosomes | 14:45 |
| fenn | i forget the purpose of using PEG | 14:45 |
| fenn | right, two cells fuse, then the fused cell drops various chromosomes but not all of them | 14:45 |
| fenn | or maybe they just don't all get transferred | 14:46 |
| yashgaroth | I suppose if the fused cell(s) manage to replicate, their nuclei would mix and you'd end up with a weird chromosome count | 14:46 |
| yashgaroth | generally you can use PEG to transfer DNA into cells, which I'm guessing was the reason in your lab | 14:48 |
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| @kanzure | yashgaroth: delinquentme nmz787__ make sure you stay through the 16th for the after-hackathon thing.. i don't know what joe is planning | 15:34 |
| nmz787 | thanks fenn... yeah forgot about PEG | 15:34 |
| nmz787 | yep | 15:35 |
| nmz787 | i'll be around til 19th or 20th prob | 15:35 |
| delinquentme | after hackaton? | 15:35 |
| delinquentme | hackathon* | 15:35 |
| nmz787 | unless i move out, lol | 15:35 |
| yashgaroth | I'll try to do 15-17th | 15:35 |
| nmz787 | hackaton works as a name too | 15:35 |
| delinquentme | its spanish? | 15:35 |
| nmz787 | PEG is used for "molecular crowding" | 15:36 |
| delinquentme | hackatón | 15:36 |
| nmz787 | i used it to make hybridomas, a cancer line fused with B cells from an immunized mouse | 15:36 |
| nmz787 | pretty cool black magic like stuff | 15:36 |
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| @kanzure | wb fenn_ | 15:53 |
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| @kanzure | where oh where did everyone go. | 15:59 |
| nmz787 | If the F-plasmid that is transferred has previously been integrated into the donor’s genome some of the donor’s chromosomal DNA may also be transferred with the plasmid DNA.[3] The amount of chromosomal DNA that is transferred depends on how long the two conjugating bacteria remain in contact. In common laboratory strains of E. coli the transfer of the entire bacterial chromosome takes about 100 minutes. The transferred | 15:59 |
| nmz787 | 100 minutes to transfer 5 Mbp | 15:59 |
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| nmz787 | 50 kbp/min | 16:00 |
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| @kanzure | obama | 16:02 |
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| @kanzure | .. damn it didn't work | 16:02 |
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| nmz787 | ? | 16:03 |
| @kanzure | nmz787: it usually sets off his crazy | 16:04 |
| nmz787 | so can we just do all this synthesis in organelles? organelle engineering sounds hard, but am I wrong? yashgaroth??? | 16:04 |
| yashgaroth | err what | 16:04 |
| yashgaroth | what synthesis | 16:04 |
| nmz787 | then screw microfluidics, its all just bio factories | 16:05 |
| nmz787 | DNA synthesis | 16:05 |
| nmz787 | random idea | 16:05 |
| yashgaroth | as much as I prefer the in vivo version whenever possible, microfluidics would be way easier | 16:05 |
| @kanzure | the chemical environment inside an organelle is inhospitable to phosphoramidite chemistry | 16:05 |
| nmz787 | not phosphoramidite | 16:05 |
| nmz787 | (i just posted a quote from wikipedia about conjugation) | 16:06 |
| nmz787 | 50kbp/min DNA translocation | 16:06 |
| @kanzure | nmz787: a netsplit means there's going to be people who don't receive the same set of messages | 16:06 |
| nmz787 | so if that could be programmed to pump one nt/time | 16:06 |
| nmz787 | then the tdt enzyme would work for synth | 16:06 |
| nmz787 | netsplit? | 16:07 |
| @kanzure | but i got your 50 kbp/min message and the ones before that | 16:07 |
| nmz787 | whats a netsplit? | 16:07 |
| @kanzure | yeah.. look near the bottom http://gnusha.org/logs/2012-05-01.log | 16:07 |
| @kanzure | http://en.wikipedia.org/wiki/Netsplit | 16:07 |
| @kanzure | "In computer networking, specifically Internet Relay Chat (IRC), netsplit is the disconnection of a given node from the previously established network or between two nodes. As an IRC network is an undirected acyclic graph, a split between any two nodes splits the entire network into two pieces." | 16:07 |
| nmz787 | what is that? | 16:07 |
| nmz787 | wait IRC is p2p? | 16:08 |
| katsmeow-afk | the servers are p2p | 16:08 |
| nmz787 | whats the freenode thing for? | 16:08 |
| katsmeow-afk | for irc | 16:08 |
| nmz787 | ? | 16:08 |
| @kanzure | freendoe is an irc network.. there are other networks like efnet and oftc | 16:08 |
| @kanzure | *freenode | 16:08 |
| nmz787 | but why are we on a network, if its p2p | 16:09 |
| @kanzure | freenode primarily hosts channels about open source projects i guess | 16:09 |
| @kanzure | the servers are p2p | 16:09 |
| * katsmeow-afk checks out freendoe | 16:09 | |
| @kanzure | clients connect to an irc server | 16:09 |
| @kanzure | for instance.. you are connected to adams.freenode.net | 16:09 |
| katsmeow-afk | nmz787, mostly because they don't want i to be split-proofed, because that wold eat bandwidth someone would haveto pay for | 16:09 |
| @kanzure | i'm connected to gibson.freenode.net out of oslo, norway | 16:09 |
| @kanzure | i think asimov.freenode.net is in houston, texas | 16:09 |
| katsmeow-afk | type: /links | 16:10 |
| nmz787 | type /links | 16:10 |
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| nmz787 | links is unknwn to pidgin | 16:10 |
| katsmeow-afk | woo, they also changed that | 16:10 |
| @kanzure | pidgin might not have /links | 16:10 |
| katsmeow-afk | ok, /raw links | 16:10 |
| @kanzure | you can do /whois nmz787 | 16:10 |
| @kanzure | anyway! | 16:11 |
| @kanzure | yes if we had an engineered enzyme we could stuff into an organelle or vesicle or something | 16:12 |
| @kanzure | or virus capsid | 16:12 |
| nmz787 | well i was specifically referring to the DNA translocation system for conjugation | 16:12 |
| nmz787 | its similar to the agrobacterium DNA injection system | 16:12 |
| nmz787 | i dont know if they actually have a locomotion step though | 16:13 |
| yashgaroth | brb | 16:13 |
| nmz787 | or maybe some sort of one-way DNA ratchet/valve.... so the DNA can only move in one direction | 16:13 |
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| nmz787 | kanzure: did it just netsplit again? | 16:35 |
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| -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware http://gnusha.org/logs/ http://bit.ly/diybionews2 http://gadaprize.org/ http://groups.google.com/group/diybio | 16:45 | |
| -!- Topic set by kanzure [~kanzure@131.252.130.248] [Mon Feb 13 12:33:19 2012] | 16:45 | |
| [Users ##hplusroadmap] | 16:45 | |
| [@kanzure ] [ chillyvanilly ] [ gnusha_ ] [ Mokbortolan_] [ roksprok ] [ thyla ] | 16:45 | |
| [ _Sketch_] [ CIA-132 ] [ Guest50608 ] [ nathaniel ] [ SDr ] [ upgrayeddd] | 16:45 | |
| [ _sol_ ] [ Coornail_ ] [ Helleshin ] [ nchaimov ] [ skorket ] [ Urchin ] | 16:45 | |
| [ AdrianG ] [ delinquentme ] [ HEx1 ] [ nmz787 ] [ splicer_ ] [ Utopiah ] | 16:45 | |
| [ AlonzoTG] [ devrandom ] [ ivan` ] [ nmz787__ ] [ strages_home] [ Vicarious ] | 16:45 | |
| [ archels ] [ drazak_ ] [ JayDugger ] [ nuba ] [ strages_shop] [ vrs ] | 16:45 | |
| [ audy ] [ ENKI-][ ] [ jennicide ] [ ParahSailin ] [ strangewarp ] [ yashgaroth] | 16:45 | |
| [ augur ] [ epitron__ ] [ jrayhawk ] [ pasky ] [ streety ] [ ybit ] | 16:45 | |
| [ bkero ] [ fenn_ ] [ katsmeow-afk] [ qnm ] [ superkuh ] [ ziyadb__ ] | 16:45 | |
| [ brownies] [ gedankenstuecke] [ kvltist ] [ rdb ] [ sylph_mako ] | 16:45 | |
| [ Cat4D ] [ gnusha ] [ Mikoangelo ] [ rkos ] [ Thorbinator_] | 16:45 | |
| -!- Irssi: ##hplusroadmap: Total of 64 nicks [1 ops, 0 halfops, 0 voices, 63 normal] | 16:45 | |
| -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 | 16:45 | |
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| @kanzure | nmz787: not sure | 18:09 |
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| nmz787 | lol | 18:23 |
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| jrayhawk | s2s is the term to use | 18:29 |
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| ParahSailin | yeah sens... | 18:46 |
| @kanzure | heh | 18:47 |
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| ParahSailin | kanzure, should i make other arrangements for the incubator? | 18:52 |
| @kanzure | ParahSailin: how big is it? | 18:53 |
| ParahSailin | i think it should be less about a yard on each side | 18:54 |
| @kanzure | i definitely can't fit something 3 meters on a side | 18:54 |
| ParahSailin | its probably pretty heavy, so forget about it | 18:56 |
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| nmz787 | kanzure: a yard ~~ 1 meter | 19:03 |
| nmz787 | not 3 | 19:03 |
| nmz787 | 3ft | 19:03 |
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| skorket | did somebody buzz me? | 19:14 |
| @kanzure | nmz787: doh i don't know what i was thinking | 19:18 |
| @kanzure | skorket: how goes the site? | 19:18 |
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| skorket | kanzure, what site? | 19:19 |
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| @kanzure | skorket: sorry, i had a brain fart | 19:27 |
| skorket | np | 19:27 |
| @kanzure | what's up? | 19:27 |
| skorket | directed at me? | 19:28 |
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| JayDugger | Before I start searching the logs, does anyone remember good discussions about applications for a Kinect? | 19:30 |
| @kanzure | JayDugger: cave mapping | 19:34 |
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| @kanzure | ParahSailin: i'm still not sure i can fit it. hm | 19:46 |
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| @kanzure | i could go either way on it | 19:54 |
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| yashgaroth | is it in working condition? I had a couple in the old lab and they broke more often than anything else | 19:57 |
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| @kanzure | i mean, i could go drive over there and see if i could fit it in, but if it doesn't that's sorta pathetic | 19:59 |
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| @kanzure | hahaha | 20:00 |
| @kanzure | DATA | 20:00 |
| @kanzure | http://www.githubarchive.org/ | 20:00 |
| @kanzure | https://github.com/blog/1112-data-at-github | 20:00 |
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| nmz787 | kanzure: i'm pretty sure an incubator will fit into a rav4 | 20:18 |
| yashgaroth | gonna need 2 people to move it | 20:19 |
| @kanzure | o.o | 20:19 |
| nmz787 | if someone could find a working rotovap for cheap, we could use that kanzure | 20:23 |
| nmz787 | for degassing stuff | 20:23 |
| nmz787 | rotovap with condenser* | 20:23 |
| nmz787 | and chiller* | 20:23 |
| nmz787 | i.e. complete | 20:23 |
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| @kanzure | nmz787: http://diyhpl.us/cgit/laser_etcher | 21:33 |
| klafka | kanzure https://github.com/jtmcmc/py-collab | 21:34 |
| klafka | -_- | 21:34 |
| @kanzure | klafka: does it work? | 21:35 |
| klafka | probably not | 21:35 |
| klafka | next step | 21:35 |
| klafka | more testing | 21:35 |
| @kanzure | haha you setup a separate repo? | 21:35 |
| @kanzure | i mean, account | 21:35 |
| klafka | huh no? | 21:35 |
| klafka | that's my only accoun | 21:35 |
| klafka | t | 21:35 |
| klafka | i think | 21:35 |
| @kanzure | github.com/ klaka | 21:35 |
| klafka | oh wait nvm | 21:35 |
| klafka | oh yah i forgot | 21:36 |
| klafka | i've been using jtmcmc because anything i may show employers/whatever | 21:36 |
| @kanzure | jrayhawk: how do i add a user to laser_etcher.git? it keeps asking me for fenn's password | 21:37 |
| jrayhawk | whaa | 21:38 |
| @kanzure | oops, i mean, it keeps asking me to be fenn | 21:38 |
| @kanzure | because i don't own the repo, even as root | 21:38 |
| jrayhawk | sudo sudo addaccess laser_etcher whomever | 21:38 |
| @kanzure | You are not the owner of that repo! | 21:39 |
| nmz787 | sudo su [git daemon user] | 21:39 |
| nmz787 | then addaccess | 21:39 |
| jrayhawk | huh, curious | 21:40 |
| nmz787 | i think that should work | 21:40 |
| jrayhawk | well, you can use adduser whomever git-laser_etcher until i work out what broke | 21:40 |
| @kanzure | dduser: The group `laser_etcher' does not exis | 21:41 |
| @kanzure | oh, git-laser_etcher | 21:41 |
| @kanzure | nmz787: well anyway. you should be able to push to nmz787@diyhpl.us:/srv/git/laser_etcher.git now | 21:43 |
| @kanzure | git remote rm origin; git remote add origin nmz787@diyhpl.us:/srv/git/laser_etcher.git | 21:43 |
| nmz787 | fenn: we need to add the plates for the gantry to the laser_etcher file | 21:43 |
| nmz787 | kanzure please try to relay that message to fenn the next time you see him on | 21:46 |
| yashgaroth | fenn_ ping, just in case | 21:47 |
| @kanzure | okie dokie | 21:47 |
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| @kanzure | klafka: you might be interested in learning about docstrings in python | 21:56 |
| klafka | oh yeah | 21:56 |
| klafka | I'm familiar with them | 21:56 |
| jrayhawk | piny bug: FIX'D | 22:05 |
| @kanzure | jrayhawk: neato, thank you | 22:09 |
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| klafka | what testing framework do you recommend kanzure for python? | 22:13 |
| klafka | unittest ? | 22:14 |
| klafka | i think i've used nose before also | 22:14 |
| gnusha_ | laser_etcher.git: 7e27143 added laser stuff to laser_etcher | 22:15 |
| @kanzure | klafka: yes i use unittest (or unittest2 if i am in python 2.6) | 22:17 |
| klafka | ah cool | 22:17 |
| klafka | man i didn't get nearly enough work done tonight | 22:17 |
| klafka | oh well | 22:17 |
| jrayhawk | i feel compelled to one-up that commit message somehow | 22:17 |
| jrayhawk | git commit -m 'this is a commit within the git version control system' | 22:18 |
| gnusha_ | laser_etcher.git: 86c7818 added google docs link to laser gantry BOM | 22:18 |
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| @kanzure | jrayhawk: "lasered lasering lasers lasered into the git distributed version control system" | 22:18 |
| @kanzure | jrayhawk: i am trying to make sure he doesn't learn about -m | 22:19 |
| @kanzure | but it looks like he might already be using it | 22:19 |
| jrayhawk | ha ha your plans are in ruin | 22:20 |
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| jrayhawk | you are right to fear man pages after all! | 22:21 |
| gnusha_ | laser_etcher.git: c09e111 added electronics dev and diag section to microfluidics.txt | 22:21 |
| @kanzure | loading bash completion features... real0m4.196s | 22:23 |
| @kanzure | damn you jblake! adding a swapfile didn't help. | 22:23 |
| jrayhawk | if you want whizbang features, use zsh | 22:24 |
| jrayhawk | bash is a dinosaur | 22:24 |
| jrayhawk | its only advantage is ubiquity | 22:24 |
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| @kanzure | http://lesswrong.com/lw/c1x/extrapolating_values_without_outsourcing/ terrifying | 23:20 |
| @kanzure | "they are going to be throwing away their precious time by working on the wrong problem (WBE rather than FAI)," | 23:20 |
| @kanzure | bleh. | 23:21 |
| @kanzure | brlcad.org is borked | 23:23 |
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| gnusha_ | nanoengineer.git: 938017b hardcoding a commit id is a bad idea | 23:29 |
| gnusha_ | pokecrystal.git: 81208d5 move the text command classes around | 23:42 |
| gnusha_ | pokecrystal.git: 6fa7fbc basic README to explain crystal.py | 23:42 |
| gnusha_ | pokecrystal.git: 0406a12 fix readme typo | 23:42 |
| gnusha_ | pokecrystal.git: b612207 clarify a sentence | 23:42 |
| gnusha_ | pokecrystal.git: 7d6626e extras/output.asm -> extras/output.txt | 23:42 |
| yashgaroth | I threw up a response to that telomerase fellow on biohack if you want something more cogent than my previous rambling | 23:43 |
| yashgaroth | turns out the vector uses a VSV coat protein that's not limited to immune cells, but everything else is still terrible about it | 23:43 |
| @kanzure | you might as well paste the link | 23:44 |
| yashgaroth | well I suppose http://discuss.biohack.me/discussion/221/safety-concerns-in-using-replication-incompetent-hiv-based-lentiviruses-for-dna-tranduction.#Item_3 | 23:45 |
| @kanzure | i promise that if i kick you i'll also kick myself, at least | 23:45 |
| yashgaroth | that was my main concern | 23:45 |
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| yashgaroth | :V | 23:47 |
| yashgaroth | I hated to discourage someone interested in biohacking, but | 23:48 |
| kanzure | perhaps he should work on a simpler project first | 23:49 |
| yashgaroth | aye | 23:50 |
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| --- Log closed Wed May 02 00:00:16 2012 | ||
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